AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006809

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006809.t1 MTR_4g082310 98.225 507 9 0 1 507 1 507 0.0 1044
MS.gene006809.t1 MTR_5g042200 79.630 486 92 3 28 506 9 494 0.0 817
MS.gene006809.t1 MTR_6g016015 59.006 483 196 2 8 488 25 507 0.0 606
MS.gene006809.t1 MTR_4g110030 61.752 468 169 4 29 488 43 508 0.0 604
MS.gene006809.t1 MTR_2g059350 57.895 494 200 4 8 499 30 517 0.0 597
MS.gene006809.t1 MTR_5g064620 48.675 415 201 4 87 489 97 511 1.03e-139 413
MS.gene006809.t1 MTR_4g125460 48.232 396 198 5 97 490 89 479 1.43e-136 404
MS.gene006809.t1 MTR_4g125460 48.232 396 198 5 97 490 228 618 8.86e-135 404
MS.gene006809.t1 MTR_4g019210 48.371 399 199 5 97 493 232 625 7.47e-134 402
MS.gene006809.t1 MTR_3g099480 47.952 415 201 5 87 489 126 537 9.19e-132 393
MS.gene006809.t1 MTR_5g081690 46.851 397 204 5 96 490 120 511 3.43e-130 389
MS.gene006809.t1 MTR_3g096020 44.519 447 211 10 70 489 150 586 7.11e-124 375
MS.gene006809.t1 MTR_3g115220 42.714 398 211 8 98 492 112 495 2.07e-99 311
MS.gene006809.t1 MTR_4g100810 40.876 411 220 8 93 497 104 497 3.97e-98 306
MS.gene006809.t1 MTR_5g099010 42.476 412 217 9 100 505 122 519 2.82e-97 305
MS.gene006809.t1 MTR_5g012770 41.304 414 209 11 92 499 119 504 1.18e-91 289
MS.gene006809.t1 MTR_5g032100 38.051 431 218 9 97 491 86 503 1.16e-90 286
MS.gene006809.t1 MTR_3g086570 39.231 390 217 7 90 464 144 528 2.04e-89 284
MS.gene006809.t1 MTR_3g100310 36.782 435 226 9 91 489 94 515 2.45e-87 278
MS.gene006809.t1 MTR_1g102030 36.739 460 243 14 50 492 57 485 8.63e-85 271
MS.gene006809.t1 MTR_4g084570 35.096 416 243 10 97 496 150 554 2.90e-81 263
MS.gene006809.t1 MTR_4g132590 37.642 441 220 11 94 489 62 492 5.92e-80 258
MS.gene006809.t1 MTR_4g132590 37.642 441 220 11 94 489 161 591 6.35e-79 258
MS.gene006809.t1 MTR_5g059260 36.650 412 231 10 98 489 158 559 3.97e-78 256
MS.gene006809.t1 MTR_2g016470 35.892 443 231 10 91 490 183 615 5.45e-78 256
MS.gene006809.t1 MTR_1g031590 34.223 412 244 9 97 492 149 549 5.77e-76 249
MS.gene006809.t1 MTR_3g005340 35.817 416 228 12 97 490 147 545 2.27e-74 246
MS.gene006809.t1 MTR_7g064210 71.687 166 37 2 212 368 24 188 1.88e-73 239
MS.gene006809.t1 MTR_3g026650 36.432 398 234 9 100 492 176 559 2.78e-71 237
MS.gene006809.t1 MTR_4g023030 36.409 401 236 8 100 495 236 622 1.32e-70 237
MS.gene006809.t1 MTR_3g086570 39.175 291 161 5 90 366 144 432 9.16e-67 222
MS.gene006809.t1 MTR_1g101990 39.249 293 158 9 206 492 3 281 2.20e-60 201
MS.gene006809.t1 MTR_3g026650 38.245 319 185 6 100 413 176 487 2.12e-59 204
MS.gene006809.t1 MTR_4g010990 33.957 374 221 9 90 457 68 421 9.94e-59 201
MS.gene006809.t1 MTR_4g010990 32.670 352 211 9 112 457 1 332 3.93e-50 175
MS.gene006809.t1 MTR_3g092590 32.209 326 192 9 78 379 65 385 4.15e-42 158
MS.gene006809.t1 MTR_3g092590 32.209 326 192 9 78 379 65 385 1.55e-41 158
MS.gene006809.t1 MTR_7g017330 31.579 304 185 7 85 370 86 384 5.40e-39 150
MS.gene006809.t1 MTR_3g026650 38.528 231 130 6 100 325 176 399 5.37e-37 141
MS.gene006809.t1 MTR_1g007390 29.819 332 198 10 78 379 65 391 1.10e-33 135
MS.gene006809.t1 MTR_1g101985 44.355 124 53 3 50 173 57 164 2.53e-25 103
MS.gene006809.t1 MTR_4g008200 26.608 342 201 13 97 427 35 337 8.91e-24 103
MS.gene006809.t1 MTR_4g008200 26.608 342 201 13 97 427 86 388 1.96e-23 102
MS.gene006809.t1 MTR_1g052225 37.195 164 53 7 95 258 19 132 2.01e-18 82.0
MS.gene006809.t1 MTR_6g046230 63.636 44 16 0 200 243 97 140 1.82e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006809.t1 AT1G14020 72.079 505 135 2 1 505 1 499 0.0 759
MS.gene006809.t1 AT2G03280 69.099 466 132 4 24 488 26 480 0.0 686
MS.gene006809.t1 AT2G03280 65.314 493 132 5 24 488 26 507 0.0 671
MS.gene006809.t1 AT5G15740 58.030 467 194 2 23 488 33 498 0.0 580
MS.gene006809.t1 AT3G02250 58.526 475 193 3 15 488 27 498 0.0 576
MS.gene006809.t1 AT2G37980 46.682 437 226 5 73 507 202 633 8.43e-139 414
MS.gene006809.t1 AT3G54100 46.296 432 215 7 73 497 198 619 1.49e-134 404
MS.gene006809.t1 AT5G35570 47.407 405 206 5 96 498 250 649 4.37e-132 398
MS.gene006809.t1 AT1G22460 46.394 416 208 5 87 490 146 558 8.14e-129 386
MS.gene006809.t1 AT5G64600 46.778 419 200 8 87 489 103 514 8.14e-129 385
MS.gene006809.t1 AT5G01100 47.132 401 204 5 97 493 216 612 6.32e-128 386
MS.gene006809.t1 AT1G76270 44.332 397 205 7 100 494 109 491 2.00e-103 321
MS.gene006809.t1 AT1G20550 42.157 408 220 7 100 505 112 505 8.80e-102 317
MS.gene006809.t1 AT4G38390 43.250 400 213 7 97 494 123 510 1.93e-101 315
MS.gene006809.t1 AT4G16650 43.521 409 207 10 89 490 113 504 2.69e-95 300
MS.gene006809.t1 AT4G16650 43.521 409 207 10 89 490 113 504 2.69e-95 300
MS.gene006809.t1 AT1G04910 36.887 469 252 11 50 506 57 493 1.19e-91 289
MS.gene006809.t1 AT3G26370 38.718 390 219 7 90 464 152 536 1.79e-89 285
MS.gene006809.t1 AT4G24530 37.674 430 219 9 97 490 101 517 3.42e-89 283
MS.gene006809.t1 AT5G65470 35.581 430 226 9 99 490 87 503 5.55e-86 274
MS.gene006809.t1 AT1G11990 39.173 411 222 10 98 491 191 590 9.16e-83 268
MS.gene006809.t1 AT1G62330 34.862 436 232 8 98 489 222 649 1.31e-82 269
MS.gene006809.t1 AT1G38065 37.046 413 230 10 97 489 36 438 3.27e-81 261
MS.gene006809.t1 AT1G38065 37.046 413 230 10 97 489 26 428 3.50e-81 260
MS.gene006809.t1 AT1G38131 37.046 413 230 10 97 489 155 557 6.60e-80 261
MS.gene006809.t1 AT1G11990 36.161 448 221 10 98 491 176 612 6.58e-78 256
MS.gene006809.t1 AT1G29200 37.931 435 219 10 97 490 70 494 3.15e-77 251
MS.gene006809.t1 AT1G29200 37.931 435 219 10 97 490 70 494 3.15e-77 251
MS.gene006809.t1 AT5G63390 38.000 400 228 8 100 493 167 552 5.54e-77 252
MS.gene006809.t1 AT1G29200 37.931 435 219 10 97 490 186 610 5.48e-76 251
MS.gene006809.t1 AT1G29200 37.762 429 216 10 103 490 2 420 3.84e-75 243
MS.gene006809.t1 AT1G35510 34.772 417 241 10 92 490 154 557 2.77e-74 245
MS.gene006809.t1 AT2G01480 36.314 369 213 7 97 449 154 516 3.87e-74 245
MS.gene006809.t1 AT1G14970 34.625 413 243 11 97 493 9 410 5.15e-74 240
MS.gene006809.t1 AT2G01480 36.695 357 207 6 97 437 154 507 1.05e-73 243
MS.gene006809.t1 AT1G14970 34.625 413 243 11 97 493 35 436 1.17e-73 240
MS.gene006809.t1 AT1G14970 34.625 413 243 11 97 493 155 556 1.50e-72 240
MS.gene006809.t1 AT2G44500 37.626 396 227 10 100 489 180 561 1.59e-72 241
MS.gene006809.t1 AT3G07900 34.951 412 243 9 84 489 180 572 1.41e-69 233
MS.gene006809.t1 AT1G52630 32.708 373 224 10 90 458 70 419 6.38e-59 201
MS.gene006809.t1 AT3G03810 36.634 303 177 7 91 379 83 384 3.80e-50 182
MS.gene006809.t1 AT3G03810 36.634 303 177 7 91 379 83 384 3.80e-50 182
MS.gene006809.t1 AT1G52630 31.054 351 218 9 112 458 1 331 1.10e-49 174
MS.gene006809.t1 AT3G26370 39.352 216 120 4 90 296 152 365 6.91e-46 165
MS.gene006809.t1 AT3G30300 32.722 327 193 8 70 379 79 395 1.31e-43 164
MS.gene006809.t1 AT3G30300 32.722 327 193 8 70 379 79 395 1.31e-43 164
MS.gene006809.t1 AT2G44500 39.914 233 127 7 100 326 180 405 1.39e-38 146
MS.gene006809.t1 AT1G51630 24.845 322 192 9 96 406 90 372 1.36e-18 88.6

Find 130 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCTGTGATGAATGATTT+TGG 0.203102 4.1:-24939763 None:intergenic
ATTGCAGCCGGTGAGATTTA+TGG 0.227102 4.1:+24939389 MS.gene006809:CDS
TGGCATTCCAATTTCTTTAC+TGG 0.232585 4.1:+24936550 MS.gene006809:CDS
TCAGATATGCATTCCCTTCA+TGG 0.261168 4.1:+24939249 MS.gene006809:intron
GATGGCTGCTTTGGATTTCA+TGG 0.269401 4.1:+24939781 MS.gene006809:CDS
AGTAAATCCAGTAAAGAAAT+TGG 0.278555 4.1:-24936557 None:intergenic
CACCCCTCAAATTGAGAATC+TGG 0.293410 4.1:+24938852 MS.gene006809:CDS
TGCTTTGGATTTCATGGTTT+CGG 0.294885 4.1:+24939787 MS.gene006809:CDS
TTCATCACAGATGGCTGCTT+TGG 0.305983 4.1:+24939772 MS.gene006809:CDS
TTCTAAGAGTGTCCTGCAAT+GGG 0.310538 4.1:+24937855 MS.gene006809:CDS
TTGCTTGATATGCATCAAAA+TGG 0.313563 4.1:+24940042 MS.gene006809:CDS
CTAAGTTTCTGTAGATCAAT+TGG 0.331955 4.1:-24938799 None:intergenic
AAGGATTTCTTATCAAGTTC+CGG 0.337356 4.1:-24938038 None:intergenic
GCTTTCTCAGGTTGTACTCT+TGG 0.343079 4.1:+24938952 MS.gene006809:CDS
AACTTGATAAGAAATCCTTT+TGG 0.353716 4.1:+24938042 MS.gene006809:CDS
TAGAAATAATCCTCCTCCTT+TGG 0.360561 4.1:-24940159 None:intergenic
TAGCGCTCATGCAGGTATTC+CGG 0.363730 4.1:+24939976 MS.gene006809:intron
CATTGTGCTGTGGACATGTT+TGG 0.367522 4.1:+24936501 MS.gene006809:CDS
ACCTTTATTCCTACCTATGA+TGG 0.388352 4.1:+24939821 MS.gene006809:CDS
AGCTAACAGGAGGCATCTCC+AGG 0.388940 4.1:-24938273 None:intergenic
ATGGACATGCTGGCTTTCTC+AGG 0.395187 4.1:+24938940 MS.gene006809:CDS
TGCCCCAGATTCTCAATTTG+AGG 0.396144 4.1:-24938856 None:intergenic
GAACCTCACATTGGTTGTTC+CGG 0.404001 4.1:+24938019 MS.gene006809:CDS
CCATCTTATTGGACCGGAAA+AGG 0.405140 4.1:+24940010 MS.gene006809:CDS
GCCTTGGTTATGCAAGCCTT+AGG 0.432969 4.1:+24939341 MS.gene006809:CDS
CCCATCATAGGTAGGAATAA+AGG 0.436770 4.1:-24939822 None:intergenic
GCAGGAAATTATACTAGTAA+TGG 0.439060 4.1:+24937830 MS.gene006809:intron
TTTCTAAGAGTGTCCTGCAA+TGG 0.444915 4.1:+24937854 MS.gene006809:CDS
ATACCTACGGTCTGCCCAAA+AGG 0.445579 4.1:-24938057 None:intergenic
AGGAAATAGTGTCTGAAGAA+AGG 0.445827 4.1:+24939279 MS.gene006809:CDS
GATTTCATGGTTTCGGTAGC+CGG 0.450519 4.1:+24939794 MS.gene006809:CDS
GGAACTGCTGTAACTCATCA+AGG 0.452325 4.1:-24939742 None:intergenic
AAAAGTCCATCTTATTGGAC+CGG 0.458534 4.1:+24940004 MS.gene006809:CDS
GAAGTGCACCACCTTGTGTT+TGG 0.463947 4.1:-24938740 None:intergenic
AAGCTGCTCCTTTGATTCCA+AGG 0.468221 4.1:+24936440 MS.gene006809:CDS
CTTACTTGAGTAGAGTTATG+AGG 0.468781 4.1:-24940237 None:intergenic
TTGGGAGACCATTATTTGCT+AGG 0.469220 4.1:-24938780 None:intergenic
GCCCCAGATTCTCAATTTGA+GGG 0.469715 4.1:-24938855 None:intergenic
GAATAAACCAAACACGAAAA+CGG 0.470528 4.1:-24936465 None:intergenic
CTAACTTGCAGGAAGTAGAA+TGG 0.473374 4.1:-24936644 None:intergenic
TAAGTTTCTGTAGATCAATT+GGG 0.475386 4.1:-24938798 None:intergenic
TGGAGATGCCTCCTGTTAGC+TGG 0.475776 4.1:+24938275 MS.gene006809:CDS
TTGTGACTGTGAGTGAACTA+TGG 0.476628 4.1:+24936530 MS.gene006809:CDS
TAACTTGCAGGAAGTAGAAT+GGG 0.479064 4.1:-24936643 None:intergenic
CAAACACGAAAACGGCTCCT+TGG 0.481546 4.1:-24936457 None:intergenic
ACCCCTCAAATTGAGAATCT+GGG 0.484445 4.1:+24938853 MS.gene006809:CDS
GAGGGGTGAATTTAAGTGCC+TGG 0.487600 4.1:-24938837 None:intergenic
GTCAAATGAAAAGTATTACT+TGG 0.489028 4.1:+24938297 MS.gene006809:CDS
CTCAGATGCAATGCCACAAA+AGG 0.490594 4.1:-24938910 None:intergenic
ATTTCCTTCTCTCTCCATGA+AGG 0.500444 4.1:-24939263 None:intergenic
GATGCACGCCTAGCAAATAA+TGG 0.504541 4.1:+24938772 MS.gene006809:CDS
ATAACCAAGGCAGTCTCCTC+CGG 0.505709 4.1:-24939329 None:intergenic
CAATTGATCTACAGAAACTT+AGG 0.505738 4.1:+24938800 MS.gene006809:CDS
ACAGGTTCACGAAAAGAGAA+TGG 0.506682 4.1:+24940101 MS.gene006809:CDS
CCGAGGATCTCAAGCGGATG+AGG 0.509548 4.1:+24938992 MS.gene006809:CDS
GTCTGAAGAAAGGAGATCCC+AGG 0.512801 4.1:+24939289 MS.gene006809:CDS
GCAGTCTCCTCCGGTGTCAA+AGG 0.517244 4.1:-24939320 None:intergenic
GTGTCAAAGGACATAGACCC+TGG 0.517880 4.1:-24939307 None:intergenic
GACACCGGAGGAGACTGCCT+TGG 0.525025 4.1:+24939325 MS.gene006809:CDS
GCATCTACAAACTCATTCCA+TGG 0.525256 4.1:-24940072 None:intergenic
CTAAGAGTGTCCTGCAATGG+GGG 0.532495 4.1:+24937857 MS.gene006809:CDS
CCTCATCCGCTTGAGATCCT+CGG 0.534275 4.1:-24938992 None:intergenic
GTTTGTAGATGCTGTGCGAC+AGG 0.534296 4.1:+24940083 MS.gene006809:CDS
CCTTTATTCCTACCTATGAT+GGG 0.535492 4.1:+24939822 MS.gene006809:CDS
TGAAAAGTATTACTTGGAGC+AGG 0.539964 4.1:+24938303 MS.gene006809:CDS
CCAAAATCATTCATCACAGA+TGG 0.542359 4.1:+24939763 MS.gene006809:CDS
TGTCTCCCGGTCAAAACCTA+AGG 0.543702 4.1:-24939357 None:intergenic
CTACAAACTCATTCCATGGA+AGG 0.551044 4.1:-24940068 None:intergenic
AAACGGCTCCTTGGAATCAA+AGG 0.552528 4.1:-24936448 None:intergenic
CCCCAGATTCTCAATTTGAG+GGG 0.555415 4.1:-24938854 None:intergenic
GAGATATGAGATGGACATGC+TGG 0.557518 4.1:+24938930 MS.gene006809:CDS
CAGGTTCACGAAAAGAGAAT+GGG 0.560835 4.1:+24940102 MS.gene006809:CDS
ATCAAAATGGAACCCTTCCA+TGG 0.561331 4.1:+24940055 MS.gene006809:CDS
CAATGTAAATCTGTGTCTCC+CGG 0.564211 4.1:-24939370 None:intergenic
TTTCCTTCTCTCTCCATGAA+GGG 0.565721 4.1:-24939262 None:intergenic
CCCTCATGAGTGCCTTTGTG+AGG 0.571802 4.1:+24940188 MS.gene006809:CDS
TCTAAGAGTGTCCTGCAATG+GGG 0.572836 4.1:+24937856 MS.gene006809:CDS
ACACAGATTTACATTGCAGC+CGG 0.575721 4.1:+24939377 MS.gene006809:CDS
GGGTCTATGTCCTTTGACAC+CGG 0.576261 4.1:+24939310 MS.gene006809:CDS
ACAATGATTCGACGATAAGT+CGG 0.577569 4.1:-24940129 None:intergenic
GTGACATGGTGACAATTGCT+CGG 0.579350 4.1:+24937991 MS.gene006809:CDS
TGGGAACATGGCAAAACTTG+TGG 0.580054 4.1:+24939841 MS.gene006809:CDS
TAGGTGCCGTGTTAATTACC+AGG 0.582283 4.1:+24938819 MS.gene006809:CDS
GATTCAGATACTTCGCGAGA+GGG 0.583952 4.1:+24938885 MS.gene006809:CDS
TGTAGACAAACCAAAGGAGG+AGG 0.587728 4.1:+24940149 MS.gene006809:CDS
TACAAACTCATTCCATGGAA+GGG 0.592326 4.1:-24940067 None:intergenic
GGTAGGAATAAAGGTGTCAC+CGG 0.594798 4.1:-24939813 None:intergenic
ACAAAGGCACTCATGAGGGT+TGG 0.596296 4.1:-24940184 None:intergenic
CCCTCACAAAGGCACTCATG+AGG 0.597403 4.1:-24940189 None:intergenic
CACACTTTGTTCCCTCACAA+AGG 0.600880 4.1:-24940200 None:intergenic
AACTTGTGGAAGGCCACCGC+AGG 0.602466 4.1:+24939855 MS.gene006809:CDS
CCTCACAAAGGCACTCATGA+GGG 0.602588 4.1:-24940188 None:intergenic
GCCATGTTCCCATCATAGGT+AGG 0.602990 4.1:-24939830 None:intergenic
CCCCTCAAATTGAGAATCTG+GGG 0.605300 4.1:+24938854 MS.gene006809:CDS
ACAACTCAACGGCCTCCTCA+AGG 0.607711 4.1:+24936595 MS.gene006809:CDS
AACATGGCAAAACTTGTGGA+AGG 0.609196 4.1:+24939845 MS.gene006809:CDS
CCTCATGAGTGCCTTTGTGA+GGG 0.609759 4.1:+24940189 MS.gene006809:CDS
TCCTACCTATGATGGGAACA+TGG 0.610654 4.1:+24939829 MS.gene006809:CDS
TTTGAATCAAATGCGTGCAG+CGG 0.612731 4.1:+24937880 MS.gene006809:CDS
ACAAAGTGTGATGATTTGCT+AGG 0.614058 4.1:+24940213 MS.gene006809:CDS
AATGGAAGTTAGATCATCAG+AGG 0.614061 4.1:+24936384 None:intergenic
GGCATTTCAGATTTGTGACA+TGG 0.614628 4.1:+24937977 MS.gene006809:intron
CATTGTAGACAAACCAAAGG+AGG 0.615228 4.1:+24940146 MS.gene006809:CDS
TTCACCACCATAAATCTCAC+CGG 0.615230 4.1:-24939396 None:intergenic
TCATTTGACCAGCTAACAGG+AGG 0.621119 4.1:-24938283 None:intergenic
ACCTAAGGCTTGCATAACCA+AGG 0.627242 4.1:-24939342 None:intergenic
GTATATATCACACAACTCAA+CGG 0.629814 4.1:+24936584 MS.gene006809:CDS
AATCATTGTAGACAAACCAA+AGG 0.637419 4.1:+24940143 MS.gene006809:CDS
ATTCAGATACTTCGCGAGAG+GGG 0.637675 4.1:+24938886 MS.gene006809:CDS
GAACTGCTGTAACTCATCAA+GGG 0.638982 4.1:-24939741 None:intergenic
TCTGAAGAAAGGAGATCCCA+GGG 0.644505 4.1:+24939290 MS.gene006809:CDS
TGTCAAAGGACATAGACCCT+GGG 0.644623 4.1:-24939306 None:intergenic
TTTGTTCAACCATTGTGCTG+TGG 0.645471 4.1:+24936491 MS.gene006809:CDS
GAAAAGTGGATACTTGACCC+TGG 0.648562 4.1:+24938255 MS.gene006809:CDS
TGATTCAGATACTTCGCGAG+AGG 0.649037 4.1:+24938884 MS.gene006809:CDS
ATTCCCTTCATGGAGAGAGA+AGG 0.650807 4.1:+24939259 MS.gene006809:CDS
CTTGCAGGAAGTAGAATGGG+AGG 0.651294 4.1:-24936640 None:intergenic
TAAGTGCCTGGTAATTAACA+CGG 0.657828 4.1:-24938825 None:intergenic
ATGGAAGTTAGATCATCAGA+GGG 0.666260 4.1:+24936385 MS.gene006809:CDS
TTGCACTAATGAAGAAGCCG+AGG 0.669813 4.1:+24938975 MS.gene006809:CDS
AATCTAACATCTCCTTGAGG+AGG 0.673975 4.1:-24936607 None:intergenic
CAAACATGTCCACAGCACAA+TGG 0.677892 4.1:-24936500 None:intergenic
AAGAAGCCGAGGATCTCAAG+CGG 0.678663 4.1:+24938986 MS.gene006809:CDS
GCTAACAGGAGGCATCTCCA+GGG 0.682429 4.1:-24938272 None:intergenic
ATTGCATCTGAGATATGAGA+TGG 0.683390 4.1:+24938921 MS.gene006809:CDS
ACAAATCTAACATCTCCTTG+AGG 0.695899 4.1:-24936610 None:intergenic
TCTATGTCCTTTGACACCGG+AGG 0.698582 4.1:+24939313 MS.gene006809:CDS
GTTCCGGAACAACCAATGTG+AGG 0.701296 4.1:-24938022 None:intergenic
GCAGCCGGTGAGATTTATGG+TGG 0.721413 4.1:+24939392 MS.gene006809:CDS
GAGTAAATAGATACCTGCGG+TGG 0.724695 4.1:-24939868 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAGATATATATTTCTTT+TGG - chr4.1:24939957-24939976 None:intergenic 10.0%
!!! GAAAAGATTTTTTTTTTTTT+TGG + chr4.1:24937079-24937098 MS.gene006809:intron 10.0%
!!! TTTAACTTTAAAAGTAATTA+TGG - chr4.1:24936906-24936925 None:intergenic 10.0%
!! ATTATGTATAATACATCAAT+CGG + chr4.1:24938359-24938378 MS.gene006809:intron 15.0%
!! ATTTAGCAATAATAGTAATA+TGG + chr4.1:24936792-24936811 MS.gene006809:intron 15.0%
!! GTCAATAATATAAACAAAAA+GGG - chr4.1:24939488-24939507 None:intergenic 15.0%
!! TCAATAATATAAACAAAAAG+GGG - chr4.1:24939487-24939506 None:intergenic 15.0%
!! TGAAAAGATATACATAAAAA+AGG - chr4.1:24939044-24939063 None:intergenic 15.0%
!! TGTCAATAATATAAACAAAA+AGG - chr4.1:24939489-24939508 None:intergenic 15.0%
!!! AATTTTCCTTTAAATATGAT+AGG + chr4.1:24937486-24937505 MS.gene006809:intron 15.0%
!!! ATATTTCTTTTGGAAATTTA+GGG - chr4.1:24939947-24939966 None:intergenic 15.0%
!!! TAAATGGATTTATTGATTTT+TGG - chr4.1:24937335-24937354 None:intergenic 15.0%
!!! TATATTTCTTTTGGAAATTT+AGG - chr4.1:24939948-24939967 None:intergenic 15.0%
!! AACATAAGTAAGTGTAATAT+TGG - chr4.1:24937644-24937663 None:intergenic 20.0%
!! ACATAAGTAAGTGTAATATT+GGG - chr4.1:24937643-24937662 None:intergenic 20.0%
!! ATATAATAGGAATAAAGAAG+TGG - chr4.1:24937927-24937946 None:intergenic 20.0%
!! ATATGTACTACTGAAAATAA+GGG - chr4.1:24938674-24938693 None:intergenic 20.0%
!! ATGGATTTAATGTATATTCA+CGG + chr4.1:24936811-24936830 MS.gene006809:intron 20.0%
!! ATTGAAGCTTATCATATAAT+AGG - chr4.1:24937940-24937959 None:intergenic 20.0%
!! CAATAATATAAACAAAAAGG+GGG - chr4.1:24939486-24939505 None:intergenic 20.0%
!! TATATGTACTACTGAAAATA+AGG - chr4.1:24938675-24938694 None:intergenic 20.0%
!! TCTAATAAAAACACAATGTT+AGG - chr4.1:24936998-24937017 None:intergenic 20.0%
!! TTATGTATAATACATCAATC+GGG + chr4.1:24938360-24938379 MS.gene006809:intron 20.0%
!! TTGCTAAATTAAATCATATG+TGG - chr4.1:24936781-24936800 None:intergenic 20.0%
!!! AAAAAAAAATCTTTTCCCAA+AGG - chr4.1:24937075-24937094 None:intergenic 20.0%
!!! AAAAAAAATCTTTTCCCAAA+GGG - chr4.1:24937074-24937093 None:intergenic 20.0%
!!! AAAAGTACAAGTTTATGATA+TGG + chr4.1:24938528-24938547 MS.gene006809:intron 20.0%
!!! AAAGTACCTATCATATTTAA+AGG - chr4.1:24937495-24937514 None:intergenic 20.0%
!!! AACATTGTGTTTTTATTAGA+TGG + chr4.1:24936998-24937017 MS.gene006809:intron 20.0%
!!! CAACAATAGTTTAAAATAAG+TGG - chr4.1:24937411-24937430 None:intergenic 20.0%
!!! TAGTATTTTAGCAACTAAAA+TGG + chr4.1:24938559-24938578 MS.gene006809:intron 20.0%
!!! TTTACTTTAGGTTAAAAAAG+AGG + chr4.1:24939709-24939728 MS.gene006809:intron 20.0%
!!! TTTTAAAAAGTCCATCTTAT+TGG + chr4.1:24939999-24940018 MS.gene006809:CDS 20.0%
!!! TTTTTTTTTTATTTTGGCAG+TGG + chr4.1:24938142-24938161 MS.gene006809:intron 20.0%
! AAAAAGTTTACTAGGAAAAG+TGG + chr4.1:24938241-24938260 MS.gene006809:CDS 25.0%
! ACAAAATACTTATACGTAGA+TGG + chr4.1:24937717-24937736 MS.gene006809:intron 25.0%
! AGTAAATCCAGTAAAGAAAT+TGG - chr4.1:24936560-24936579 None:intergenic 25.0%
! ATCTAATATAGAAGCTATTC+TGG + chr4.1:24939908-24939927 MS.gene006809:intron 25.0%
! ATTCCACTTTACTTTACTTT+AGG + chr4.1:24939697-24939716 MS.gene006809:intron 25.0%
! CAAAATACTTATACGTAGAT+GGG + chr4.1:24937718-24937737 MS.gene006809:intron 25.0%
! CAATGTTTAATTACCAGATT+TGG + chr4.1:24937956-24937975 MS.gene006809:intron 25.0%
! CATAACAAATGATATACCTA+CGG - chr4.1:24938073-24938092 None:intergenic 25.0%
! CATAAGTAAGTGTAATATTG+GGG - chr4.1:24937642-24937661 None:intergenic 25.0%
! GATGGTCAATATCAATTAAT+TGG - chr4.1:24937191-24937210 None:intergenic 25.0%
! GCAACACAAATTATAAAAGA+AGG - chr4.1:24939079-24939098 None:intergenic 25.0%
! GTTTATCAACACATACATTA+TGG - chr4.1:24937052-24937071 None:intergenic 25.0%
! TAACCTAAAGTAAAGTAAAG+TGG - chr4.1:24939703-24939722 None:intergenic 25.0%
! TAAGTTTCTGTAGATCAATT+GGG - chr4.1:24938801-24938820 None:intergenic 25.0%
! TGGTTAATGATTCTTTGATA+TGG + chr4.1:24938422-24938441 MS.gene006809:intron 25.0%
! TTAAAGTTTGATACAGTGAT+TGG + chr4.1:24938402-24938421 MS.gene006809:intron 25.0%
!! AACTTGATAAGAAATCCTTT+TGG + chr4.1:24938042-24938061 MS.gene006809:CDS 25.0%
!! ACTTGATAAGAAATCCTTTT+GGG + chr4.1:24938043-24938062 MS.gene006809:CDS 25.0%
!! ACTTTTCCTAGTAAACTTTT+TGG - chr4.1:24938242-24938261 None:intergenic 25.0%
!! CAAACATTTTTAATGATCTG+AGG - chr4.1:24936876-24936895 None:intergenic 25.0%
!! CTTTTCCTAGTAAACTTTTT+GGG - chr4.1:24938241-24938260 None:intergenic 25.0%
!! TATATCTTTTCATGAGCAAT+AGG + chr4.1:24939052-24939071 MS.gene006809:intron 25.0%
!! TTAGGTTAAAAAAGAGGTAT+TGG + chr4.1:24939715-24939734 MS.gene006809:intron 25.0%
!! TTTAGAGTTATTAGCAGATA+TGG + chr4.1:24938462-24938481 MS.gene006809:intron 25.0%
!!! AACAATGATAAAGTGCTTTT+TGG - chr4.1:24938614-24938633 None:intergenic 25.0%
!!! ATATTGACCATCATGTTTTT+TGG + chr4.1:24937199-24937218 MS.gene006809:intron 25.0%
!!! ATGCAAGATAGTTTTACATA+AGG + chr4.1:24937679-24937698 MS.gene006809:intron 25.0%
!!! GATTTTTGGTTAGAATCTAA+TGG - chr4.1:24937321-24937340 None:intergenic 25.0%
!!! GTCAAATGAAAAGTATTACT+TGG + chr4.1:24938297-24938316 MS.gene006809:CDS 25.0%
!!! TAAGATGGACTTTTTAAAAC+CGG - chr4.1:24939998-24940017 None:intergenic 25.0%
!!! TACTTGGCTGTTATTTTTTA+CGG - chr4.1:24937265-24937284 None:intergenic 25.0%
!!! TGCAAGATAGTTTTACATAA+GGG + chr4.1:24937680-24937699 MS.gene006809:intron 25.0%
!!! TTTTCTGCTTGTTATATAGT+AGG + chr4.1:24937102-24937121 MS.gene006809:intron 25.0%
AAATTCTACTGAACAGCTAA+AGG - chr4.1:24937471-24937490 None:intergenic 30.0%
AAGGATTTCTTATCAAGTTC+CGG - chr4.1:24938041-24938060 None:intergenic 30.0%
AATATAAACAAAAAGGGGGA+GGG - chr4.1:24939482-24939501 None:intergenic 30.0%
AATCATTGTAGACAAACCAA+AGG + chr4.1:24940143-24940162 MS.gene006809:CDS 30.0%
AGACTTCACTCTAAACTAAA+TGG - chr4.1:24937351-24937370 None:intergenic 30.0%
CAATTGATCTACAGAAACTT+AGG + chr4.1:24938800-24938819 MS.gene006809:CDS 30.0%
CTAAGTTTCTGTAGATCAAT+TGG - chr4.1:24938802-24938821 None:intergenic 30.0%
GAATAAACCAAACACGAAAA+CGG - chr4.1:24936468-24936487 None:intergenic 30.0%
GCAGGAAATTATACTAGTAA+TGG + chr4.1:24937830-24937849 MS.gene006809:intron 30.0%
GGGAGGATACAAATTATAAA+TGG - chr4.1:24939573-24939592 None:intergenic 30.0%
GTACAAGTTTATGATATGGA+TGG + chr4.1:24938532-24938551 MS.gene006809:intron 30.0%
GTATATATCACACAACTCAA+CGG + chr4.1:24936584-24936603 MS.gene006809:CDS 30.0%
TAATAGGAATAAAGAAGTGG+TGG - chr4.1:24937924-24937943 None:intergenic 30.0%
TAATATAAACAAAAAGGGGG+AGG - chr4.1:24939483-24939502 None:intergenic 30.0%
TACTACTGAAAATAAGGGAA+GGG - chr4.1:24938669-24938688 None:intergenic 30.0%
TATATACAAGGACTCATACA+GGG - chr4.1:24937560-24937579 None:intergenic 30.0%
TGATGGGGATATTATATACA+AGG - chr4.1:24937572-24937591 None:intergenic 30.0%
TTAGGATGAAGAAATGCATA+AGG - chr4.1:24939652-24939671 None:intergenic 30.0%
TTATATACAAGGACTCATAC+AGG - chr4.1:24937561-24937580 None:intergenic 30.0%
TTGCTTGATATGCATCAAAA+TGG + chr4.1:24940042-24940061 MS.gene006809:CDS 30.0%
! AATGGTGAACTCACAATTTT+TGG - chr4.1:24939452-24939471 None:intergenic 30.0%
! AGATAGAAAAACTAACTTGC+AGG - chr4.1:24936658-24936677 None:intergenic 30.0%
! ATTAAGAGTCTTGTGTTCAA+AGG + chr4.1:24939161-24939180 MS.gene006809:intron 30.0%
! TAAAAAAGGCATAGCAAAAC+AGG - chr4.1:24939030-24939049 None:intergenic 30.0%
! TATGTGTTGATAAACCCTTT+GGG + chr4.1:24937057-24937076 MS.gene006809:intron 30.0%
!! AATGTTTTTGTTTGCTTGAG+TGG + chr4.1:24936699-24936718 MS.gene006809:intron 30.0%
!! GTTGCATGATTTGTTGAATT+TGG + chr4.1:24939094-24939113 MS.gene006809:intron 30.0%
!!! AATAAGTGGATCCACTTTTT+TGG - chr4.1:24937397-24937416 None:intergenic 30.0%
!!! ATAAGTGGATCCACTTTTTT+GGG - chr4.1:24937396-24937415 None:intergenic 30.0%
AAAAGTCCATCTTATTGGAC+CGG + chr4.1:24940004-24940023 MS.gene006809:CDS 35.0%
AACAAGCAGTAACCATGATT+AGG - chr4.1:24939670-24939689 None:intergenic 35.0%
ACAAATCTAACATCTCCTTG+AGG - chr4.1:24936613-24936632 None:intergenic 35.0%
ACAATGATTCGACGATAAGT+CGG - chr4.1:24940132-24940151 None:intergenic 35.0%
ACCTTTATTCCTACCTATGA+TGG + chr4.1:24939821-24939840 MS.gene006809:CDS 35.0%
ACTACTGAAAATAAGGGAAG+GGG - chr4.1:24938668-24938687 None:intergenic 35.0%
AGGAAATAGTGTCTGAAGAA+AGG + chr4.1:24939279-24939298 MS.gene006809:CDS 35.0%
AGGAAGTCCAAAAAACATGA+TGG - chr4.1:24937209-24937228 None:intergenic 35.0%
ATCATGCTTAGCTGAATGTT+GGG + chr4.1:24937026-24937045 MS.gene006809:intron 35.0%
ATGGAAGTTAGATCATCAGA+GGG + chr4.1:24936385-24936404 MS.gene006809:CDS 35.0%
ATTTGCTTTCTGTTAAGTGC+TGG - chr4.1:24937601-24937620 None:intergenic 35.0%
ATTTGTGTGAAGAAACAGGT+AGG + chr4.1:24937132-24937151 MS.gene006809:intron 35.0%
CAAAGCAAATCATCTCTAAC+AGG - chr4.1:24937229-24937248 None:intergenic 35.0%
CCAAAATCATTCATCACAGA+TGG + chr4.1:24939763-24939782 MS.gene006809:CDS 35.0%
CCTTTATTCCTACCTATGAT+GGG + chr4.1:24939822-24939841 MS.gene006809:CDS 35.0%
CTTACTTGAGTAGAGTTATG+AGG - chr4.1:24940240-24940259 None:intergenic 35.0%
GAGTTCCCAAAAAGTTTACT+AGG + chr4.1:24938233-24938252 MS.gene006809:CDS 35.0%
GCATTTCTTCATCCTAATCA+TGG + chr4.1:24939655-24939674 MS.gene006809:intron 35.0%
GGGGTACACTAAAAAGATTA+GGG - chr4.1:24937376-24937395 None:intergenic 35.0%
GTAATGATCTGACAAATGAG+AGG + chr4.1:24939189-24939208 MS.gene006809:intron 35.0%
GTACTACTGAAAATAAGGGA+AGG - chr4.1:24938670-24938689 None:intergenic 35.0%
GTAGATTTGTGTGAAGAAAC+AGG + chr4.1:24937128-24937147 MS.gene006809:intron 35.0%
TAAGTGCCTGGTAATTAACA+CGG - chr4.1:24938828-24938847 None:intergenic 35.0%
TACAAACTCATTCCATGGAA+GGG - chr4.1:24940070-24940089 None:intergenic 35.0%
TAGAAATAATCCTCCTCCTT+TGG - chr4.1:24940162-24940181 None:intergenic 35.0%
TGGCATTCCAATTTCTTTAC+TGG + chr4.1:24936550-24936569 MS.gene006809:CDS 35.0%
TGGGGTACACTAAAAAGATT+AGG - chr4.1:24937377-24937396 None:intergenic 35.0%
TTTAGTGTACCCCAAAAAAG+TGG + chr4.1:24937383-24937402 MS.gene006809:intron 35.0%
! ATTGCATCTGAGATATGAGA+TGG + chr4.1:24938921-24938940 MS.gene006809:CDS 35.0%
! CAAATCTGAAATGCCAAATC+TGG - chr4.1:24937972-24937991 None:intergenic 35.0%
! CCATCTGTGATGAATGATTT+TGG - chr4.1:24939766-24939785 None:intergenic 35.0%
! GTATGTGTTGATAAACCCTT+TGG + chr4.1:24937056-24937075 MS.gene006809:intron 35.0%
! TAAAGAAGTGGTGGAAGAAT+AGG - chr4.1:24937915-24937934 None:intergenic 35.0%
! TAACTTGCAGGAAGTAGAAT+GGG - chr4.1:24936646-24936665 None:intergenic 35.0%
! TACTTATACGTAGATGGGTT+TGG + chr4.1:24937723-24937742 MS.gene006809:intron 35.0%
! TGCTTTGGATTTCATGGTTT+CGG + chr4.1:24939787-24939806 MS.gene006809:CDS 35.0%
! TTTTCATTTGACCAGCTAAC+AGG - chr4.1:24938289-24938308 None:intergenic 35.0%
!! AATCCAAGTGCTGAAAAATG+GGG - chr4.1:24939593-24939612 None:intergenic 35.0%
!! ACAAAGTGTGATGATTTGCT+AGG + chr4.1:24940213-24940232 MS.gene006809:CDS 35.0%
!! ATTTGCTTTGTTGCGTTTTG+TGG + chr4.1:24937239-24937258 MS.gene006809:intron 35.0%
!! CAATCCAAGTGCTGAAAAAT+GGG - chr4.1:24939594-24939613 None:intergenic 35.0%
!! TCAATCCAAGTGCTGAAAAA+TGG - chr4.1:24939595-24939614 None:intergenic 35.0%
!! TGAAAAGTATTACTTGGAGC+AGG + chr4.1:24938303-24938322 MS.gene006809:CDS 35.0%
!!! TAAGTGGATCCACTTTTTTG+GGG - chr4.1:24937395-24937414 None:intergenic 35.0%
AACATGGCAAAACTTGTGGA+AGG + chr4.1:24939845-24939864 MS.gene006809:CDS 40.0%
AATCTAACATCTCCTTGAGG+AGG - chr4.1:24936610-24936629 None:intergenic 40.0%
ACACAGATTTACATTGCAGC+CGG + chr4.1:24939377-24939396 MS.gene006809:CDS 40.0%
ACAGGTTCACGAAAAGAGAA+TGG + chr4.1:24940101-24940120 MS.gene006809:CDS 40.0%
ACCCCTCAAATTGAGAATCT+GGG + chr4.1:24938853-24938872 MS.gene006809:CDS 40.0%
ACCTTGTGTTTGGCAAAAAG+CGG - chr4.1:24938733-24938752 None:intergenic 40.0%
AGCACTTGGATTGAAAGTTG+TGG + chr4.1:24939601-24939620 MS.gene006809:intron 40.0%
ATCAAAATGGAACCCTTCCA+TGG + chr4.1:24940055-24940074 MS.gene006809:CDS 40.0%
ATCTATCCCTACTCAGTACT+TGG - chr4.1:24937281-24937300 None:intergenic 40.0%
ATTTCCTTCTCTCTCCATGA+AGG - chr4.1:24939266-24939285 None:intergenic 40.0%
CAATGTAAATCTGTGTCTCC+CGG - chr4.1:24939373-24939392 None:intergenic 40.0%
CACAAGCTAGAATCATTCAG+TGG - chr4.1:24939535-24939554 None:intergenic 40.0%
CAGGTTCACGAAAAGAGAAT+GGG + chr4.1:24940102-24940121 MS.gene006809:CDS 40.0%
CATTGTAGACAAACCAAAGG+AGG + chr4.1:24940146-24940165 MS.gene006809:CDS 40.0%
CCCATCATAGGTAGGAATAA+AGG - chr4.1:24939825-24939844 None:intergenic 40.0%
CGTGAGTAAATAGATACCTG+CGG - chr4.1:24939874-24939893 None:intergenic 40.0%
CTACAAACTCATTCCATGGA+AGG - chr4.1:24940071-24940090 None:intergenic 40.0%
CTACTGAAAATAAGGGAAGG+GGG - chr4.1:24938667-24938686 None:intergenic 40.0%
CTCATACAGGGTCATTGAAA+TGG - chr4.1:24937548-24937567 None:intergenic 40.0%
GAACTGCTGTAACTCATCAA+GGG - chr4.1:24939744-24939763 None:intergenic 40.0%
GATCATGCTTAGCTGAATGT+TGG + chr4.1:24937025-24937044 MS.gene006809:intron 40.0%
GCATCTACAAACTCATTCCA+TGG - chr4.1:24940075-24940094 None:intergenic 40.0%
GGCATTTCAGATTTGTGACA+TGG + chr4.1:24937977-24937996 MS.gene006809:intron 40.0%
GGTTTGGAAATGTGTAGCTT+TGG + chr4.1:24936733-24936752 MS.gene006809:intron 40.0%
TACGTAGCTTAACCCAAAGT+TGG + chr4.1:24938586-24938605 MS.gene006809:intron 40.0%
TACTGAAAATAAGGGAAGGG+GGG - chr4.1:24938666-24938685 None:intergenic 40.0%
TATCTTATTAGCGCTCATGC+AGG + chr4.1:24939968-24939987 MS.gene006809:intron 40.0%
TCAGATATGCATTCCCTTCA+TGG + chr4.1:24939249-24939268 MS.gene006809:intron 40.0%
TTCACCACCATAAATCTCAC+CGG - chr4.1:24939399-24939418 None:intergenic 40.0%
TTGGGAGACCATTATTTGCT+AGG - chr4.1:24938783-24938802 None:intergenic 40.0%
TTGTGACTGTGAGTGAACTA+TGG + chr4.1:24936530-24936549 MS.gene006809:CDS 40.0%
TTTCCTTCTCTCTCCATGAA+GGG - chr4.1:24939265-24939284 None:intergenic 40.0%
TTTGAATCAAATGCGTGCAG+CGG + chr4.1:24937880-24937899 MS.gene006809:CDS 40.0%
TTTGTTCAACCATTGTGCTG+TGG + chr4.1:24936491-24936510 MS.gene006809:CDS 40.0%
! CTAACTTGCAGGAAGTAGAA+TGG - chr4.1:24936647-24936666 None:intergenic 40.0%
! GCAATTGTACAAGCCTTTTC+CGG - chr4.1:24940026-24940045 None:intergenic 40.0%
! TTTTGCCATGTTCCCATCAT+AGG - chr4.1:24939837-24939856 None:intergenic 40.0%
!! ATAACAGCCAAGTACTGAGT+AGG + chr4.1:24937271-24937290 MS.gene006809:intron 40.0%
!! GAAAGTTGTGGCTCTGAAAA+TGG + chr4.1:24939613-24939632 MS.gene006809:intron 40.0%
!! TAACAGCCAAGTACTGAGTA+GGG + chr4.1:24937272-24937291 MS.gene006809:intron 40.0%
!! TAAGTGCTGGAGAGATTGAT+GGG - chr4.1:24937588-24937607 None:intergenic 40.0%
!! TGCTTTTTGGCACCAACTTT+GGG - chr4.1:24938601-24938620 None:intergenic 40.0%
!! TTAAGTGCTGGAGAGATTGA+TGG - chr4.1:24937589-24937608 None:intergenic 40.0%
!! TTCTAAGAGTGTCCTGCAAT+GGG + chr4.1:24937855-24937874 MS.gene006809:CDS 40.0%
!! TTTCTAAGAGTGTCCTGCAA+TGG + chr4.1:24937854-24937873 MS.gene006809:CDS 40.0%
!!! AGTGGTTTTGTGATGTGGTT+TGG + chr4.1:24936717-24936736 MS.gene006809:intron 40.0%
AAACGGCTCCTTGGAATCAA+AGG - chr4.1:24936451-24936470 None:intergenic 45.0%
AAGCTGCTCCTTTGATTCCA+AGG + chr4.1:24936440-24936459 MS.gene006809:CDS 45.0%
AAGGGGGAGGGAGATATAAA+TGG - chr4.1:24939470-24939489 None:intergenic 45.0%
ACCTAAGGCTTGCATAACCA+AGG - chr4.1:24939345-24939364 None:intergenic 45.0%
ATTCAGATACTTCGCGAGAG+GGG + chr4.1:24938886-24938905 MS.gene006809:CDS 45.0%
ATTCCCTTCATGGAGAGAGA+AGG + chr4.1:24939259-24939278 MS.gene006809:CDS 45.0%
ATTGCAGCCGGTGAGATTTA+TGG + chr4.1:24939389-24939408 MS.gene006809:CDS 45.0%
CAAACATGTCCACAGCACAA+TGG - chr4.1:24936503-24936522 None:intergenic 45.0%
CACACTTTGTTCCCTCACAA+AGG - chr4.1:24940203-24940222 None:intergenic 45.0%
CACCCCTCAAATTGAGAATC+TGG + chr4.1:24938852-24938871 MS.gene006809:CDS 45.0%
CAGCTAAAGGAGTTGTACAG+TGG - chr4.1:24937458-24937477 None:intergenic 45.0%
CATTGTGCTGTGGACATGTT+TGG + chr4.1:24936501-24936520 MS.gene006809:CDS 45.0%
CCATCTTATTGGACCGGAAA+AGG + chr4.1:24940010-24940029 MS.gene006809:CDS 45.0%
CCCCAGATTCTCAATTTGAG+GGG - chr4.1:24938857-24938876 None:intergenic 45.0%
CCCCTCAAATTGAGAATCTG+GGG + chr4.1:24938854-24938873 MS.gene006809:CDS 45.0%
CTCAGATGCAATGCCACAAA+AGG - chr4.1:24938913-24938932 None:intergenic 45.0%
CTGCAAGATTTCAGCTGACA+AGG - chr4.1:24937814-24937833 None:intergenic 45.0%
CTTGTCAGCTGAAATCTTGC+AGG + chr4.1:24937812-24937831 MS.gene006809:intron 45.0%
GAAAAGTGGATACTTGACCC+TGG + chr4.1:24938255-24938274 MS.gene006809:CDS 45.0%
GAGATATGAGATGGACATGC+TGG + chr4.1:24938930-24938949 MS.gene006809:CDS 45.0%
GAGTAAATAGATACCTGCGG+TGG - chr4.1:24939871-24939890 None:intergenic 45.0%
GAGTTGTACAGTGGAGAATC+TGG - chr4.1:24937449-24937468 None:intergenic 45.0%
GATGCACGCCTAGCAAATAA+TGG + chr4.1:24938772-24938791 MS.gene006809:CDS 45.0%
GATTCAGATACTTCGCGAGA+GGG + chr4.1:24938885-24938904 MS.gene006809:CDS 45.0%
GATTTCATGGTTTCGGTAGC+CGG + chr4.1:24939794-24939813 MS.gene006809:CDS 45.0%
GCCCCAGATTCTCAATTTGA+GGG - chr4.1:24938858-24938877 None:intergenic 45.0%
GGAACTGCTGTAACTCATCA+AGG - chr4.1:24939745-24939764 None:intergenic 45.0%
GTACAGTGGAGAATCTGGAT+GGG - chr4.1:24937444-24937463 None:intergenic 45.0%
GTGACATGGTGACAATTGCT+CGG + chr4.1:24937991-24938010 MS.gene006809:CDS 45.0%
TAAGGGAAGGGGGGAAATAT+AGG - chr4.1:24938657-24938676 None:intergenic 45.0%
TCATTTGACCAGCTAACAGG+AGG - chr4.1:24938286-24938305 None:intergenic 45.0%
TCTGAAGAAAGGAGATCCCA+GGG + chr4.1:24939290-24939309 MS.gene006809:CDS 45.0%
TGATTCAGATACTTCGCGAG+AGG + chr4.1:24938884-24938903 MS.gene006809:CDS 45.0%
TGCCCCAGATTCTCAATTTG+AGG - chr4.1:24938859-24938878 None:intergenic 45.0%
TGGGAACATGGCAAAACTTG+TGG + chr4.1:24939841-24939860 MS.gene006809:CDS 45.0%
TGTACAGTGGAGAATCTGGA+TGG - chr4.1:24937445-24937464 None:intergenic 45.0%
TGTAGACAAACCAAAGGAGG+AGG + chr4.1:24940149-24940168 MS.gene006809:CDS 45.0%
TGTCAAAGGACATAGACCCT+GGG - chr4.1:24939309-24939328 None:intergenic 45.0%
TTCATCACAGATGGCTGCTT+TGG + chr4.1:24939772-24939791 MS.gene006809:CDS 45.0%
TTGATTCAAACCCCCATTGC+AGG - chr4.1:24937870-24937889 None:intergenic 45.0%
TTGCACTAATGAAGAAGCCG+AGG + chr4.1:24938975-24938994 MS.gene006809:CDS 45.0%
! ACGTAGATGGGTTTGGTAGA+CGG + chr4.1:24937730-24937749 MS.gene006809:intron 45.0%
! CCTTTTCCGGTCCAATAAGA+TGG - chr4.1:24940013-24940032 None:intergenic 45.0%
! GAACCTCACATTGGTTGTTC+CGG + chr4.1:24938019-24938038 MS.gene006809:CDS 45.0%
! GATGGCTGCTTTGGATTTCA+TGG + chr4.1:24939781-24939800 MS.gene006809:CDS 45.0%
! GCTTTCTCAGGTTGTACTCT+TGG + chr4.1:24938952-24938971 MS.gene006809:CDS 45.0%
! GCTTTTTGCCAAACACAAGG+TGG + chr4.1:24938732-24938751 MS.gene006809:CDS 45.0%
! TAGGTGCCGTGTTAATTACC+AGG + chr4.1:24938819-24938838 MS.gene006809:CDS 45.0%
! TCCTACCTATGATGGGAACA+TGG + chr4.1:24939829-24939848 MS.gene006809:CDS 45.0%
! TCGGCTTTTGAACCTCACAT+TGG + chr4.1:24938010-24938029 MS.gene006809:CDS 45.0%
!! AAGTGCTGGAGAGATTGATG+GGG - chr4.1:24937587-24937606 None:intergenic 45.0%
!! GGTAGGAATAAAGGTGTCAC+CGG - chr4.1:24939816-24939835 None:intergenic 45.0%
!! GTGCTTTTTGGCACCAACTT+TGG - chr4.1:24938602-24938621 None:intergenic 45.0%
!! GTTGTGGCTCTGAAAATGGT+TGG + chr4.1:24939617-24939636 MS.gene006809:intron 45.0%
!! TCTAAGAGTGTCCTGCAATG+GGG + chr4.1:24937856-24937875 MS.gene006809:CDS 45.0%
!! TGCAAGCCTTAGGTTTTGAC+CGG + chr4.1:24939351-24939370 MS.gene006809:CDS 45.0%
!!! ACCGCTTTTTGCCAAACACA+AGG + chr4.1:24938729-24938748 MS.gene006809:CDS 45.0%
!!! GCTTGAGTGGTTTTGTGATG+TGG + chr4.1:24936712-24936731 MS.gene006809:intron 45.0%
!!! TTATTTTGGCAGTGGCTTCG+AGG + chr4.1:24938150-24938169 MS.gene006809:intron 45.0%
!!! AAAGTTTTTTTTTTTTATTT+TGG + chr4.1:24938136-24938155 MS.gene006809:intron 5.0%
AAGAAGCCGAGGATCTCAAG+CGG + chr4.1:24938986-24939005 MS.gene006809:CDS 50.0%
ATAACCAAGGCAGTCTCCTC+CGG - chr4.1:24939332-24939351 None:intergenic 50.0%
ATACCTACGGTCTGCCCAAA+AGG - chr4.1:24938060-24938079 None:intergenic 50.0%
CAAACACGAAAACGGCTCCT+TGG - chr4.1:24936460-24936479 None:intergenic 50.0%
CCTCATGAGTGCCTTTGTGA+GGG + chr4.1:24940189-24940208 MS.gene006809:CDS 50.0%
CTTGCAGGAAGTAGAATGGG+AGG - chr4.1:24936643-24936662 None:intergenic 50.0%
GAAGTGCACCACCTTGTGTT+TGG - chr4.1:24938743-24938762 None:intergenic 50.0%
GAGGGGTGAATTTAAGTGCC+TGG - chr4.1:24938840-24938859 None:intergenic 50.0%
GCCATGTTCCCATCATAGGT+AGG - chr4.1:24939833-24939852 None:intergenic 50.0%
GCCTTGGTTATGCAAGCCTT+AGG + chr4.1:24939341-24939360 MS.gene006809:CDS 50.0%
GGGTCTATGTCCTTTGACAC+CGG + chr4.1:24939310-24939329 MS.gene006809:CDS 50.0%
GTCTGAAGAAAGGAGATCCC+AGG + chr4.1:24939289-24939308 MS.gene006809:CDS 50.0%
GTGTCAAAGGACATAGACCC+TGG - chr4.1:24939310-24939329 None:intergenic 50.0%
GTTCCGGAACAACCAATGTG+AGG - chr4.1:24938025-24938044 None:intergenic 50.0%
GTTTGTAGATGCTGTGCGAC+AGG + chr4.1:24940083-24940102 MS.gene006809:CDS 50.0%
TAGCGCTCATGCAGGTATTC+CGG + chr4.1:24939976-24939995 MS.gene006809:intron 50.0%
TCTATGTCCTTTGACACCGG+AGG + chr4.1:24939313-24939332 MS.gene006809:CDS 50.0%
TGTCTCCCGGTCAAAACCTA+AGG - chr4.1:24939360-24939379 None:intergenic 50.0%
! AATCCTTTTGGGCAGACCGT+AGG + chr4.1:24938054-24938073 MS.gene006809:CDS 50.0%
! CGTAGATGGGTTTGGTAGAC+GGG + chr4.1:24937731-24937750 MS.gene006809:intron 50.0%
! GTAGATGGGTTTGGTAGACG+GGG + chr4.1:24937732-24937751 MS.gene006809:intron 50.0%
!! ACAAAGGCACTCATGAGGGT+TGG - chr4.1:24940187-24940206 None:intergenic 50.0%
!! ATGGACATGCTGGCTTTCTC+AGG + chr4.1:24938940-24938959 MS.gene006809:CDS 50.0%
!! CAAGGAGCCGTTTTCGTGTT+TGG + chr4.1:24936458-24936477 MS.gene006809:CDS 50.0%
!! CCAAGTGCTGAAAAATGGGG+AGG - chr4.1:24939590-24939609 None:intergenic 50.0%
!! CCTCACAAAGGCACTCATGA+GGG - chr4.1:24940191-24940210 None:intergenic 50.0%
!! CCTCCCCATTTTTCAGCACT+TGG + chr4.1:24939587-24939606 MS.gene006809:intron 50.0%
!! CTAAGAGTGTCCTGCAATGG+GGG + chr4.1:24937857-24937876 MS.gene006809:CDS 50.0%
!! GCAAGCCTTAGGTTTTGACC+GGG + chr4.1:24939352-24939371 MS.gene006809:CDS 50.0%
ACAACTCAACGGCCTCCTCA+AGG + chr4.1:24936595-24936614 MS.gene006809:CDS 55.0%
CCCTCATGAGTGCCTTTGTG+AGG + chr4.1:24940188-24940207 MS.gene006809:CDS 55.0%
CCTCATCCGCTTGAGATCCT+CGG - chr4.1:24938995-24939014 None:intergenic 55.0%
GCAGCCGGTGAGATTTATGG+TGG + chr4.1:24939392-24939411 MS.gene006809:CDS 55.0%
TGGAGATGCCTCCTGTTAGC+TGG + chr4.1:24938275-24938294 MS.gene006809:CDS 55.0%
!! AGCTAACAGGAGGCATCTCC+AGG - chr4.1:24938276-24938295 None:intergenic 55.0%
!! CCCTCACAAAGGCACTCATG+AGG - chr4.1:24940192-24940211 None:intergenic 55.0%
!! GCTAACAGGAGGCATCTCCA+GGG - chr4.1:24938275-24938294 None:intergenic 55.0%
AACTTGTGGAAGGCCACCGC+AGG + chr4.1:24939855-24939874 MS.gene006809:CDS 60.0%
CCGAGGATCTCAAGCGGATG+AGG + chr4.1:24938992-24939011 MS.gene006809:CDS 60.0%
! GCAGTCTCCTCCGGTGTCAA+AGG - chr4.1:24939323-24939342 None:intergenic 60.0%
! TCGCGAGAGGGGACCTTTTG+TGG + chr4.1:24938897-24938916 MS.gene006809:CDS 60.0%
GACACCGGAGGAGACTGCCT+TGG + chr4.1:24939325-24939344 MS.gene006809:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 24936385 24940263 24936385 ID=MS.gene006809
chr4.1 mRNA 24936385 24940263 24936385 ID=MS.gene006809.t1;Parent=MS.gene006809
chr4.1 exon 24936385 24936661 24936385 ID=MS.gene006809.t1.exon1;Parent=MS.gene006809.t1
chr4.1 CDS 24936385 24936661 24936385 ID=cds.MS.gene006809.t1;Parent=MS.gene006809.t1
chr4.1 exon 24937834 24937901 24937834 ID=MS.gene006809.t1.exon2;Parent=MS.gene006809.t1
chr4.1 CDS 24937834 24937901 24937834 ID=cds.MS.gene006809.t1;Parent=MS.gene006809.t1
chr4.1 exon 24937987 24938075 24937987 ID=MS.gene006809.t1.exon3;Parent=MS.gene006809.t1
chr4.1 CDS 24937987 24938075 24937987 ID=cds.MS.gene006809.t1;Parent=MS.gene006809.t1
chr4.1 exon 24938162 24938324 24938162 ID=MS.gene006809.t1.exon4;Parent=MS.gene006809.t1
chr4.1 CDS 24938162 24938324 24938162 ID=cds.MS.gene006809.t1;Parent=MS.gene006809.t1
chr4.1 exon 24938724 24939013 24938724 ID=MS.gene006809.t1.exon5;Parent=MS.gene006809.t1
chr4.1 CDS 24938724 24939013 24938724 ID=cds.MS.gene006809.t1;Parent=MS.gene006809.t1
chr4.1 exon 24939253 24939457 24939253 ID=MS.gene006809.t1.exon6;Parent=MS.gene006809.t1
chr4.1 CDS 24939253 24939457 24939253 ID=cds.MS.gene006809.t1;Parent=MS.gene006809.t1
chr4.1 exon 24939719 24939876 24939719 ID=MS.gene006809.t1.exon7;Parent=MS.gene006809.t1
chr4.1 CDS 24939719 24939876 24939719 ID=cds.MS.gene006809.t1;Parent=MS.gene006809.t1
chr4.1 exon 24939990 24940263 24939990 ID=MS.gene006809.t1.exon8;Parent=MS.gene006809.t1
chr4.1 CDS 24939990 24940263 24939990 ID=cds.MS.gene006809.t1;Parent=MS.gene006809.t1
Gene Sequence

>MS.gene006809

ATGGAAGTTAGATCATCAGAGGGTAATCAAGTGAGATATTATGATAAGCTTCTTCAAGCTGCTCCTTTGATTCCAAGGAGCCGTTTTCGTGTTTGGTTTATTCGAATTTGTTCAACCATTGTGCTGTGGACATGTTTGGTTCAGCTTGTGACTGTGAGTGAACTATGGCATTCCAATTTCTTTACTGGATTTACTAGTCGTATATATCACACAACTCAACGGCCTCCTCAAGGAGATGTTAGATTTGTTCAATCTCCTCCCATTCTACTTCCTGCAAGTTAGTTTTTCTATCTACTTATTTTTCTCAAGATTGCAATGTTTTTGTTTGCTTGAGTGGTTTTGTGATGTGGTTTGGAAATGTGTAGCTTTGGTTGAATCTATGATGCAAACTCTCCACATATGATTTAATTTAGCAATAATAGTAATATGGATTTAATGTATATTCACGGTTAGTGTAAAGATTTTTTACACTGCGAATCAATCATTATCCTCAGATCATTAAAAATGTTTGACTTCAACCATAATTACTTTTAAAGTTAAACATATGATTAGTAGTGTTTTGTTTTGACAATATATCAAAATTCAAATATCATAATATTTATATGATCAACCTAACATTGTGTTTTTATTAGATGGTAATGATCATGCTTAGCTGAATGTTGGGCCATAATGTATGTGTTGATAAACCCTTTGGGAAAAGATTTTTTTTTTTTTTGGTTTTCTGCTTGTTATATAGTAGGTTTGTAGATTTGTGTGAAGAAACAGGTAGGAGCATGAATGTTAGTGACTTACAATTGTTAATACCAATTAATTGATATTGACCATCATGTTTTTTGGACTTCCTGTTAGAGATGATTTGCTTTGTTGCGTTTTGTGGCCGTAAAAAATAACAGCCAAGTACTGAGTAGGGATAGATACATAGATCAGTTGATGCCATTAGATTCTAACCAAAAATCAATAAATCCATTTAGTTTAGAGTGAAGTCTTTCCCTAATCTTTTTAGTGTACCCCAAAAAAGTGGATCCACTTATTTTAAACTATTGTTGATACTTAATGCCCATCCAGATTCTCCACTGTACAACTCCTTTAGCTGTTCAGTAGAATTTTCCTTTAAATATGATAGGTACTTTTTTATCTCAAATTATGTTTGAAGCATTTGTCCATTTCAATGACCCTGTATGAGTCCTTGTATATAATATCCCCATCAATCTCTCCAGCACTTAACAGAAAGCAAATAAGAGTCTAACTGAAGATCCCCAATATTACACTTACTTATGTTGAGACATGCATATGCATGCAAGATAGTTTTACATAAGGGTATAAGTAAAAAAAACAAAATACTTATACGTAGATGGGTTTGGTAGACGGGGTATGAGTATCATTAGCTGTTTATATCTTTTCTGATGCACTTTTGTAAATATTAAAGCCTTGTCAGCTGAAATCTTGCAGGAAATTATACTAGTAATGGTTTTCTAAGAGTGTCCTGCAATGGGGGTTTGAATCAAATGCGTGCAGCGGTTTGTACTTCCTATTCTTCCACCACTTCTTTATTCCTATTATATGATAAGCTTCAATGTTTAATTACCAGATTTGGCATTTCAGATTTGTGACATGGTGACAATTGCTCGGCTTTTGAACCTCACATTGGTTGTTCCGGAACTTGATAAGAAATCCTTTTGGGCAGACCGTAGGTATATCATTTGTTATGAAAAAAAAGAGTATTCTCTAATTTGCTTGCGTTGTGTCATGTTAAAGTTTTTTTTTTTTATTTTGGCAGTGGCTTCGAGGATATATTTGATGTAAGACATTTCATTGATTCTTTACGAGATGAAGTTCGAATAGTGAAAAGAGTTCCCAAAAAGTTTACTAGGAAAAGTGGATACTTGACCCTGGAGATGCCTCCTGTTAGCTGGTCAAATGAAAAGTATTACTTGGAGCAGGTCTGCAGATTCTATTTGTTGCACTTAATCTAAAATTATGTATAATACATCAATCGGGTTTTCTCTTATTTGACAATTTAAAGTTTGATACAGTGATTGGTTAATGATTCTTTGATATGGCTTTTAAGACAGTGACATTTAGAGTTATTAGCAGATATGGAAACATTTTATAATATCTTGAAATATTAATCATTCATATGAAGAAAAGTACAAGTTTATGATATGGATGGCTTCTAGTATTTTAGCAACTAAAATGGATTGTACGTAGCTTAACCCAAAGTTGGTGCCAAAAAGCACTTTATCATTGTTGAAGTGAAAAACTATAGTTACCTATATTTCCCCCCTTCCCTTATTTTCAGTAGTACATATATTGAGTTTGACTATCGACATTAACTGCAGATTCTACCGCTTTTTGCCAAACACAAGGTGGTGCACTTCAACAAAACAGATGCACGCCTAGCAAATAATGGTCTCCCAATTGATCTACAGAAACTTAGGTGCCGTGTTAATTACCAGGCACTTAAATTCACCCCTCAAATTGAGAATCTGGGGCAAAAATTGATTCAGATACTTCGCGAGAGGGGACCTTTTGTGGCATTGCATCTGAGATATGAGATGGACATGCTGGCTTTCTCAGGTTGTACTCTTGGTTGCACTAATGAAGAAGCCGAGGATCTCAAGCGGATGAGGTATTTTATGTTTCCTGTTTTGCTATGCCTTTTTTATGTATATCTTTTCATGAGCAATAGGTCCTTCTTTTATAATTTGTGTTGCATGATTTGTTGAATTTGGCATATTTTGACAACTACAAATTAAGTACATTCACTTTATAAAAGATTAAGAGTCTTGTGTTCAAAGGAATTTGTAATGATCTGACAAATGAGAGGTCTATCTAAGATAAAATTCTGATTTTGCTTGCATTATTCAGATATGCATTCCCTTCATGGAGAGAGAAGGAAATAGTGTCTGAAGAAAGGAGATCCCAGGGTCTATGTCCTTTGACACCGGAGGAGACTGCCTTGGTTATGCAAGCCTTAGGTTTTGACCGGGAGACACAGATTTACATTGCAGCCGGTGAGATTTATGGTGGTGAACGTAGACTTGCGCAACTGAGAGCTGCATTTCCAAAAATTGTGAGTTCACCATTTATATCTCCCTCCCCCTTTTTGTTTATATTATTGACATTGACATTTGATATAATAAAGTACCACTGAATGATTCTAGCTTGTGCTTATGCACGATTTTCCATTTATAATTTGTATCCTCCCCATTTTTCAGCACTTGGATTGAAAGTTGTGGCTCTGAAAATGGTTGGCTTAATCTACCTTATGCATTTCTTCATCCTAATCATGGTTACTGCTTGTTTGATAAGATTCCACTTTACTTTACTTTAGGTTAAAAAAGAGGTATTGGTCGCCCTTGATGAGTTACAGCAGTTCCAAAATCATTCATCACAGATGGCTGCTTTGGATTTCATGGTTTCGGTAGCCGGTGACACCTTTATTCCTACCTATGATGGGAACATGGCAAAACTTGTGGAAGGCCACCGCAGGTATCTATTTACTCACGCTAGTTGCAATTTCATCTAATATAGAAGCTATTCTGGTTTTAAAAAACATCCCTAAATTTCCAAAAGAAATATATATCTTATTAGCGCTCATGCAGGTATTCCGGTTTTAAAAAGTCCATCTTATTGGACCGGAAAAGGCTTGTACAATTGCTTGATATGCATCAAAATGGAACCCTTCCATGGAATGAGTTTGTAGATGCTGTGCGACAGGTTCACGAAAAGAGAATGGGTCAGCCGACTTATCGTCGAATCATTGTAGACAAACCAAAGGAGGAGGATTATTTCTACGCCAACCCTCATGAGTGCCTTTGTGAGGGAACAAAGTGTGATGATTTGCTAGGTCCTCATAACTCTACTCAAGTAAGATGA
Protein sequence

>MS.gene006809.t1

MEVRSSEGNQVRYYDKLLQAAPLIPRSRFRVWFIRICSTIVLWTCLVQLVTVSELWHSNFFTGFTSRIYHTTQRPPQGDVRFVQSPPILLPARNYTSNGFLRVSCNGGLNQMRAAICDMVTIARLLNLTLVVPELDKKSFWADRSGFEDIFDVRHFIDSLRDEVRIVKRVPKKFTRKSGYLTLEMPPVSWSNEKYYLEQILPLFAKHKVVHFNKTDARLANNGLPIDLQKLRCRVNYQALKFTPQIENLGQKLIQILRERGPFVALHLRYEMDMLAFSGCTLGCTNEEAEDLKRMRYAFPSWREKEIVSEERRSQGLCPLTPEETALVMQALGFDRETQIYIAAGEIYGGERRLAQLRAAFPKIVKKEVLVALDELQQFQNHSSQMAALDFMVSVAGDTFIPTYDGNMAKLVEGHRRYSGFKKSILLDRKRLVQLLDMHQNGTLPWNEFVDAVRQVHEKRMGQPTYRRIIVDKPKEEDYFYANPHECLCEGTKCDDLLGPHNSTQVR