AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene031478

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031478.t1 MTR_4g082310 98.028 507 10 0 1 507 1 507 0.0 1042
MS.gene031478.t1 MTR_5g042200 79.835 486 91 3 28 506 9 494 0.0 822
MS.gene031478.t1 MTR_6g016015 59.298 484 193 3 8 488 25 507 0.0 604
MS.gene031478.t1 MTR_4g110030 61.884 467 170 3 29 488 43 508 0.0 603
MS.gene031478.t1 MTR_2g059350 57.980 495 198 4 8 499 30 517 0.0 597
MS.gene031478.t1 MTR_5g064620 48.675 415 201 4 87 489 97 511 2.59e-140 414
MS.gene031478.t1 MTR_4g125460 48.485 396 197 5 97 490 89 479 2.81e-137 405
MS.gene031478.t1 MTR_4g125460 48.485 396 197 5 97 490 228 618 1.52e-135 405
MS.gene031478.t1 MTR_4g019210 48.371 399 199 5 97 493 232 625 4.17e-134 402
MS.gene031478.t1 MTR_3g099480 47.952 415 201 5 87 489 126 537 1.84e-131 392
MS.gene031478.t1 MTR_5g081690 46.599 397 205 5 96 490 120 511 5.71e-130 388
MS.gene031478.t1 MTR_3g096020 45.735 422 200 8 87 489 175 586 1.39e-123 374
MS.gene031478.t1 MTR_3g115220 43.358 399 207 9 98 492 112 495 8.59e-100 312
MS.gene031478.t1 MTR_4g100810 40.876 411 220 8 93 497 104 497 3.53e-98 306
MS.gene031478.t1 MTR_5g099010 42.476 412 217 9 100 505 122 519 3.27e-97 305
MS.gene031478.t1 MTR_5g012770 41.304 414 209 11 92 499 119 504 1.31e-91 289
MS.gene031478.t1 MTR_5g032100 38.051 431 218 9 97 491 86 503 1.59e-90 286
MS.gene031478.t1 MTR_3g086570 39.487 390 216 7 90 464 144 528 2.99e-89 284
MS.gene031478.t1 MTR_3g100310 36.782 435 226 9 91 489 94 515 1.06e-87 279
MS.gene031478.t1 MTR_1g102030 36.304 460 245 13 50 492 57 485 1.01e-84 271
MS.gene031478.t1 MTR_4g084570 35.096 416 243 10 97 496 150 554 9.04e-82 265
MS.gene031478.t1 MTR_4g132590 37.642 441 220 11 94 489 62 492 1.44e-79 257
MS.gene031478.t1 MTR_5g059260 36.650 412 231 10 98 489 158 559 1.01e-78 257
MS.gene031478.t1 MTR_4g132590 37.642 441 220 11 94 489 161 591 1.78e-78 257
MS.gene031478.t1 MTR_2g016470 35.892 443 231 10 91 490 183 615 4.66e-78 256
MS.gene031478.t1 MTR_1g031590 34.223 412 244 9 97 492 149 549 1.77e-76 250
MS.gene031478.t1 MTR_3g005340 35.507 414 232 11 97 490 147 545 3.68e-74 245
MS.gene031478.t1 MTR_7g064210 71.687 166 37 2 212 368 24 188 1.35e-73 239
MS.gene031478.t1 MTR_3g026650 36.432 398 234 9 100 492 176 559 1.70e-71 238
MS.gene031478.t1 MTR_4g023030 36.409 401 236 8 100 495 236 622 8.19e-71 237
MS.gene031478.t1 MTR_3g086570 39.519 291 160 5 90 366 144 432 1.37e-66 221
MS.gene031478.t1 MTR_1g101990 39.249 293 158 9 206 492 3 281 2.23e-60 201
MS.gene031478.t1 MTR_3g026650 38.245 319 185 6 100 413 176 487 1.11e-59 204
MS.gene031478.t1 MTR_4g010990 33.957 374 221 9 90 457 68 421 9.83e-59 201
MS.gene031478.t1 MTR_4g010990 32.670 352 211 9 112 457 1 332 3.47e-50 176
MS.gene031478.t1 MTR_3g092590 32.686 309 180 8 94 379 82 385 2.02e-42 159
MS.gene031478.t1 MTR_3g092590 32.686 309 180 8 94 379 82 385 1.05e-41 158
MS.gene031478.t1 MTR_7g017330 31.148 305 185 7 85 370 86 384 4.82e-39 151
MS.gene031478.t1 MTR_3g026650 38.528 231 130 6 100 325 176 399 2.89e-37 142
MS.gene031478.t1 MTR_1g007390 30.159 315 186 9 94 379 82 391 8.28e-34 135
MS.gene031478.t1 MTR_1g101985 42.742 124 55 2 50 173 57 164 4.44e-25 102
MS.gene031478.t1 MTR_4g008200 26.608 342 201 13 97 427 35 337 4.50e-24 103
MS.gene031478.t1 MTR_4g008200 26.608 342 201 13 97 427 86 388 1.08e-23 103
MS.gene031478.t1 MTR_1g052225 37.195 164 53 7 95 258 19 132 2.01e-18 82.0
MS.gene031478.t1 MTR_6g046230 38.318 107 58 2 145 243 34 140 4.35e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene031478.t1 AT1G14020 72.079 505 135 2 1 505 1 499 0.0 759
MS.gene031478.t1 AT2G03280 69.099 466 132 4 24 488 26 480 0.0 687
MS.gene031478.t1 AT2G03280 65.314 493 132 5 24 488 26 507 0.0 672
MS.gene031478.t1 AT5G15740 58.030 467 194 2 23 488 33 498 0.0 581
MS.gene031478.t1 AT3G02250 58.491 477 190 4 15 488 27 498 0.0 576
MS.gene031478.t1 AT2G37980 46.453 437 227 5 73 507 202 633 1.70e-137 411
MS.gene031478.t1 AT3G54100 47.330 412 205 6 93 497 213 619 1.55e-133 401
MS.gene031478.t1 AT5G35570 47.407 405 206 5 96 498 250 649 2.75e-132 398
MS.gene031478.t1 AT1G22460 45.349 430 215 6 73 490 137 558 2.95e-129 387
MS.gene031478.t1 AT5G64600 46.778 419 200 8 87 489 103 514 2.62e-128 384
MS.gene031478.t1 AT5G01100 47.132 401 204 5 97 493 216 612 3.31e-128 387
MS.gene031478.t1 AT1G76270 44.584 397 204 7 100 494 109 491 1.95e-104 324
MS.gene031478.t1 AT4G38390 43.500 400 212 7 97 494 123 510 1.77e-102 318
MS.gene031478.t1 AT1G20550 42.157 408 220 7 100 505 112 505 7.90e-102 317
MS.gene031478.t1 AT4G16650 43.521 409 207 10 89 490 113 504 2.63e-95 300
MS.gene031478.t1 AT4G16650 43.521 409 207 10 89 490 113 504 2.63e-95 300
MS.gene031478.t1 AT1G04910 36.638 464 260 10 50 506 57 493 1.49e-91 289
MS.gene031478.t1 AT4G24530 37.674 430 219 9 97 490 101 517 1.25e-89 284
MS.gene031478.t1 AT3G26370 38.619 391 218 8 90 464 152 536 2.92e-89 284
MS.gene031478.t1 AT5G65470 35.581 430 226 9 99 490 87 503 1.83e-86 275
MS.gene031478.t1 AT1G11990 39.173 411 222 10 98 491 191 590 2.90e-82 267
MS.gene031478.t1 AT1G62330 34.862 436 232 8 98 489 222 649 4.50e-82 268
MS.gene031478.t1 AT1G38065 37.046 413 230 10 97 489 26 428 8.13e-82 262
MS.gene031478.t1 AT1G38065 37.046 413 230 10 97 489 36 438 8.37e-82 262
MS.gene031478.t1 AT1G38131 37.046 413 230 10 97 489 155 557 2.27e-80 262
MS.gene031478.t1 AT1G11990 36.161 448 221 10 98 491 176 612 2.07e-77 254
MS.gene031478.t1 AT5G63390 38.000 400 228 9 100 493 167 552 2.50e-76 250
MS.gene031478.t1 AT1G29200 37.931 435 219 11 97 490 70 494 2.57e-76 248
MS.gene031478.t1 AT1G29200 37.931 435 219 11 97 490 70 494 2.57e-76 248
MS.gene031478.t1 AT1G35510 35.012 417 240 10 92 490 154 557 3.04e-75 248
MS.gene031478.t1 AT1G29200 37.931 435 219 11 97 490 186 610 5.28e-75 248
MS.gene031478.t1 AT2G01480 36.314 369 213 7 97 449 154 516 8.88e-75 246
MS.gene031478.t1 AT1G14970 34.140 413 245 10 97 493 9 410 1.63e-74 241
MS.gene031478.t1 AT2G01480 36.695 357 207 6 97 437 154 507 2.22e-74 244
MS.gene031478.t1 AT1G14970 34.140 413 245 10 97 493 35 436 2.51e-74 242
MS.gene031478.t1 AT1G29200 37.762 429 216 11 103 490 2 420 3.78e-74 241
MS.gene031478.t1 AT1G14970 34.140 413 245 10 97 493 155 556 3.45e-73 242
MS.gene031478.t1 AT2G44500 37.626 396 227 10 100 489 180 561 8.49e-73 241
MS.gene031478.t1 AT3G07900 34.772 394 241 7 100 489 191 572 1.03e-68 231
MS.gene031478.t1 AT1G52630 32.708 373 224 10 90 458 70 419 4.11e-59 202
MS.gene031478.t1 AT3G03810 35.855 304 178 7 91 379 83 384 7.97e-50 181
MS.gene031478.t1 AT3G03810 35.855 304 178 7 91 379 83 384 7.97e-50 181
MS.gene031478.t1 AT1G52630 31.054 351 218 9 112 458 1 331 9.08e-50 174
MS.gene031478.t1 AT3G26370 39.171 217 119 5 90 296 152 365 1.14e-45 164
MS.gene031478.t1 AT3G30300 33.333 306 180 7 91 379 97 395 3.36e-43 163
MS.gene031478.t1 AT3G30300 33.333 306 180 7 91 379 97 395 3.36e-43 163
MS.gene031478.t1 AT2G44500 39.914 233 127 7 100 326 180 405 4.72e-39 147
MS.gene031478.t1 AT1G51630 24.845 322 192 9 96 406 90 372 7.23e-19 89.4

Find 129 sgRNAs with CRISPR-Local

Find 285 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCTGTGATGAATGATTT+TGG 0.203102 4.2:-27531529 None:intergenic
ATTGCAGCCGGTGAGATTTA+TGG 0.227102 4.2:+27531156 MS.gene031478:CDS
TGGCATTCCAATTTCTTTAC+TGG 0.232585 4.2:+27528316 MS.gene031478:CDS
TCAGATATGCATTCCCTTCA+TGG 0.261168 4.2:+27531016 MS.gene031478:intron
AGTAAATCCAGTAAAGAAAT+TGG 0.278555 4.2:-27528323 None:intergenic
CACCCCTCAAATTGAGAATC+TGG 0.293410 4.2:+27530619 MS.gene031478:CDS
GATGGCTGCGTTGGATTTCA+TGG 0.305662 4.2:+27531547 MS.gene031478:CDS
TTCTAAGAGTGTCCTGCAAT+GGG 0.310538 4.2:+27529622 MS.gene031478:CDS
TTGCTTGATATGCATCAAAA+TGG 0.313563 4.2:+27531808 MS.gene031478:CDS
CTAAGTTTCTGTAGATCAAT+TGG 0.331955 4.2:-27530566 None:intergenic
AAGGATTTCTTATCAAGTTC+CGG 0.337356 4.2:-27529805 None:intergenic
GCTTTCTCAGGTTGTACTCT+TGG 0.343079 4.2:+27530719 MS.gene031478:CDS
AACTTGATAAGAAATCCTTT+TGG 0.353716 4.2:+27529809 MS.gene031478:CDS
TAGAAATAATCCTCCTCCTT+TGG 0.360561 4.2:-27531925 None:intergenic
TAGCTCTCATGCAGGTATTC+CGG 0.361952 4.2:+27531742 MS.gene031478:intron
TGCGTTGGATTTCATGGTTT+CGG 0.364096 4.2:+27531553 MS.gene031478:CDS
CATTGTGCTGTGGACATGTT+TGG 0.367522 4.2:+27528267 MS.gene031478:CDS
ACCTTTATTCCTACCTATGA+TGG 0.388352 4.2:+27531587 MS.gene031478:CDS
AGCTAACAGGAGGCATCTCC+AGG 0.388940 4.2:-27530040 None:intergenic
ATGGACATGCTGGCTTTCTC+AGG 0.395187 4.2:+27530707 MS.gene031478:CDS
TGCCCCAGATTCTCAATTTG+AGG 0.396144 4.2:-27530623 None:intergenic
GAACCTCACATTGGTTGTTC+CGG 0.404001 4.2:+27529786 MS.gene031478:CDS
CCATCTTATTGGACCGGAAA+AGG 0.405140 4.2:+27531776 MS.gene031478:CDS
GCCTTGGTTATGCAAGCCTT+AGG 0.432969 4.2:+27531108 MS.gene031478:CDS
CCCATCATAGGTAGGAATAA+AGG 0.436770 4.2:-27531588 None:intergenic
GCAGGAAATTATACTAGTAA+TGG 0.439060 4.2:+27529597 MS.gene031478:intron
TTTCTAAGAGTGTCCTGCAA+TGG 0.444915 4.2:+27529621 MS.gene031478:CDS
ATACCTACGGTCTGCCCAAA+AGG 0.445579 4.2:-27529824 None:intergenic
AGGAAATAGTGTCTGAAGAA+AGG 0.445827 4.2:+27531046 MS.gene031478:CDS
GATTTCATGGTTTCGGTAGC+CGG 0.450519 4.2:+27531560 MS.gene031478:CDS
GGAACTGCTGTAACTCATCA+AGG 0.452325 4.2:-27531508 None:intergenic
AAAAGTCCATCTTATTGGAC+CGG 0.458534 4.2:+27531770 MS.gene031478:CDS
GAAGTGCACCACCTTGTGTT+TGG 0.463947 4.2:-27530507 None:intergenic
AAGCTGCTCCTTTGATTCCA+AGG 0.468221 4.2:+27528206 MS.gene031478:CDS
TTCATCACAGATGGCTGCGT+TGG 0.468716 4.2:+27531538 MS.gene031478:CDS
CTTACTTGAGTAGAGTTATG+AGG 0.468781 4.2:-27532003 None:intergenic
TTGGGAGACCATTATTTGCT+AGG 0.469220 4.2:-27530547 None:intergenic
GCCCCAGATTCTCAATTTGA+GGG 0.469715 4.2:-27530622 None:intergenic
TTATTCTGGCAGTGGCTTCG+AGG 0.470272 4.2:+27529917 MS.gene031478:intron
GAATAAACCAAACACGAAAA+CGG 0.470528 4.2:-27528231 None:intergenic
CTAACTTGCAGGAAGTAGAA+TGG 0.473374 4.2:-27528410 None:intergenic
TAAGTTTCTGTAGATCAATT+GGG 0.475386 4.2:-27530565 None:intergenic
TGGAGATGCCTCCTGTTAGC+TGG 0.475776 4.2:+27530042 MS.gene031478:CDS
TTGTGACTGTGAGTGAACTA+TGG 0.476628 4.2:+27528296 MS.gene031478:CDS
TAACTTGCAGGAAGTAGAAT+GGG 0.479064 4.2:-27528409 None:intergenic
CAAACACGAAAACGGCTCCT+TGG 0.481546 4.2:-27528223 None:intergenic
ACCCCTCAAATTGAGAATCT+GGG 0.484445 4.2:+27530620 MS.gene031478:CDS
GAGGGGTGAATTTAAGTGCC+TGG 0.487600 4.2:-27530604 None:intergenic
GTCAAATGAAAAGTATTACT+TGG 0.489028 4.2:+27530064 MS.gene031478:CDS
CTCAGATGCAATGCCACAAA+AGG 0.490594 4.2:-27530677 None:intergenic
ATTTCCTTCTCTCTCCATGA+AGG 0.500444 4.2:-27531030 None:intergenic
GATGCACGCCTAGCAAATAA+TGG 0.504541 4.2:+27530539 MS.gene031478:CDS
ATAACCAAGGCAGTCTCCTC+CGG 0.505709 4.2:-27531096 None:intergenic
CAATTGATCTACAGAAACTT+AGG 0.505738 4.2:+27530567 MS.gene031478:CDS
GTCTGAAGAAAGGAGATCAC+AGG 0.505868 4.2:+27531056 MS.gene031478:CDS
ACAGGTTCACGAAAAGAGAA+TGG 0.506682 4.2:+27531867 MS.gene031478:CDS
CCGAGGATCTCAAGCGGATG+AGG 0.509548 4.2:+27530759 MS.gene031478:CDS
GCAGTCTCCTCCGGTGTCAA+AGG 0.517244 4.2:-27531087 None:intergenic
GACACCGGAGGAGACTGCCT+TGG 0.525025 4.2:+27531092 MS.gene031478:CDS
GCATCTACAAACTCATTCCA+TGG 0.525256 4.2:-27531838 None:intergenic
CTAAGAGTGTCCTGCAATGG+GGG 0.532495 4.2:+27529624 MS.gene031478:CDS
CCTCATCCGCTTGAGATCCT+CGG 0.534275 4.2:-27530759 None:intergenic
GTTTGTAGATGCTGTGCGAC+AGG 0.534296 4.2:+27531849 MS.gene031478:CDS
CCTTTATTCCTACCTATGAT+GGG 0.535492 4.2:+27531588 MS.gene031478:CDS
TGAAAAGTATTACTTGGAGC+AGG 0.539964 4.2:+27530070 MS.gene031478:CDS
CCAAAATCATTCATCACAGA+TGG 0.542359 4.2:+27531529 MS.gene031478:CDS
TGTCTCCCGGTCAAAACCTA+AGG 0.543702 4.2:-27531124 None:intergenic
CTACAAACTCATTCCATGGA+AGG 0.551044 4.2:-27531834 None:intergenic
AAACGGCTCCTTGGAATCAA+AGG 0.552528 4.2:-27528214 None:intergenic
CCCCAGATTCTCAATTTGAG+GGG 0.555415 4.2:-27530621 None:intergenic
GAGATATGAGATGGACATGC+TGG 0.557518 4.2:+27530697 MS.gene031478:CDS
CAGGTTCACGAAAAGAGAAT+GGG 0.560835 4.2:+27531868 MS.gene031478:CDS
ATCAAAATGGAACCCTTCCA+TGG 0.561331 4.2:+27531821 MS.gene031478:CDS
CAATGTAAATCTGTGTCTCC+CGG 0.564211 4.2:-27531137 None:intergenic
TTTCCTTCTCTCTCCATGAA+GGG 0.565721 4.2:-27531029 None:intergenic
CCCTCATGAGTGCCTTTGTG+AGG 0.571802 4.2:+27531954 MS.gene031478:CDS
TCTAAGAGTGTCCTGCAATG+GGG 0.572836 4.2:+27529623 MS.gene031478:CDS
ACACAGATTTACATTGCAGC+CGG 0.575721 4.2:+27531144 MS.gene031478:CDS
ACAATGATTCGACGATAAGT+CGG 0.577569 4.2:-27531895 None:intergenic
GTGACATGGTGACAATTGCT+CGG 0.579350 4.2:+27529758 MS.gene031478:CDS
TGGGAACATGGCAAAACTTG+TGG 0.580054 4.2:+27531607 MS.gene031478:CDS
TAGGTGCCGTGTTAATTACC+AGG 0.582283 4.2:+27530586 MS.gene031478:CDS
GATTCAGATACTTCGCGAGA+GGG 0.583952 4.2:+27530652 MS.gene031478:CDS
TGTAGACAAACCAAAGGAGG+AGG 0.587728 4.2:+27531915 MS.gene031478:CDS
GGGTCTATGTCCTTTGACAC+CGG 0.590187 4.2:+27531077 MS.gene031478:CDS
TACAAACTCATTCCATGGAA+GGG 0.592326 4.2:-27531833 None:intergenic
GGTAGGAATAAAGGTGTCAC+CGG 0.594798 4.2:-27531579 None:intergenic
ACAAAGGCACTCATGAGGGT+TGG 0.596296 4.2:-27531950 None:intergenic
TTTGAATCAAATGCGTGCCG+CGG 0.597362 4.2:+27529647 MS.gene031478:CDS
CCCTCACAAAGGCACTCATG+AGG 0.597403 4.2:-27531955 None:intergenic
CACACTTTGTTCCCTCACAA+AGG 0.600880 4.2:-27531966 None:intergenic
AACTTGTGGAAGGCCACCGC+AGG 0.602466 4.2:+27531621 MS.gene031478:CDS
CCTCACAAAGGCACTCATGA+GGG 0.602588 4.2:-27531954 None:intergenic
GCCATGTTCCCATCATAGGT+AGG 0.602990 4.2:-27531596 None:intergenic
CCCCTCAAATTGAGAATCTG+GGG 0.605300 4.2:+27530621 MS.gene031478:CDS
ACAACTCAACGGCCTCCTCA+AGG 0.607711 4.2:+27528361 MS.gene031478:CDS
AACATGGCAAAACTTGTGGA+AGG 0.609196 4.2:+27531611 MS.gene031478:CDS
CCTCATGAGTGCCTTTGTGA+GGG 0.609759 4.2:+27531955 MS.gene031478:CDS
TCCTACCTATGATGGGAACA+TGG 0.610654 4.2:+27531595 MS.gene031478:CDS
ACAAAGTGTGATGATTTGCT+AGG 0.614058 4.2:+27531979 MS.gene031478:CDS
AATGGAAGTTAGATCATCAG+AGG 0.614061 4.2:+27528150 None:intergenic
GGCATTTCAGATTTGTGACA+TGG 0.614628 4.2:+27529744 MS.gene031478:intron
CATTGTAGACAAACCAAAGG+AGG 0.615228 4.2:+27531912 MS.gene031478:CDS
TTCACCACCATAAATCTCAC+CGG 0.615230 4.2:-27531163 None:intergenic
TCATTTGACCAGCTAACAGG+AGG 0.621119 4.2:-27530050 None:intergenic
ACCTAAGGCTTGCATAACCA+AGG 0.627242 4.2:-27531109 None:intergenic
GTATATATCACACAACTCAA+CGG 0.629814 4.2:+27528350 MS.gene031478:CDS
GAAAAGTGGATACTCGACCC+TGG 0.636896 4.2:+27530022 MS.gene031478:CDS
AATCATTGTAGACAAACCAA+AGG 0.637419 4.2:+27531909 MS.gene031478:CDS
ATTCAGATACTTCGCGAGAG+GGG 0.637675 4.2:+27530653 MS.gene031478:CDS
GAACTGCTGTAACTCATCAA+GGG 0.638982 4.2:-27531507 None:intergenic
TTTGTTCAACCATTGTGCTG+TGG 0.645471 4.2:+27528257 MS.gene031478:CDS
TGATTCAGATACTTCGCGAG+AGG 0.649037 4.2:+27530651 MS.gene031478:CDS
ATTCCCTTCATGGAGAGAGA+AGG 0.650807 4.2:+27531026 MS.gene031478:CDS
CTTGCAGGAAGTAGAATGGG+AGG 0.651294 4.2:-27528406 None:intergenic
GAGTAAATATATACCTGCGG+TGG 0.652300 4.2:-27531634 None:intergenic
TAAGTGCCTGGTAATTAACA+CGG 0.657828 4.2:-27530592 None:intergenic
ATGGAAGTTAGATCATCAGA+GGG 0.666260 4.2:+27528151 MS.gene031478:CDS
TTGCACTAATGAAGAAGCCG+AGG 0.669813 4.2:+27530742 MS.gene031478:CDS
AATATAACATCTCCTTGAGG+AGG 0.676147 4.2:-27528373 None:intergenic
CAAACATGTCCACAGCACAA+TGG 0.677892 4.2:-27528266 None:intergenic
AAGAAGCCGAGGATCTCAAG+CGG 0.678663 4.2:+27530753 MS.gene031478:CDS
TCTGAAGAAAGGAGATCACA+GGG 0.680384 4.2:+27531057 MS.gene031478:CDS
GCTAACAGGAGGCATCTCCA+GGG 0.682429 4.2:-27530039 None:intergenic
ATTGCATCTGAGATATGAGA+TGG 0.683390 4.2:+27530688 MS.gene031478:CDS
ACAAATATAACATCTCCTTG+AGG 0.683422 4.2:-27528376 None:intergenic
TCTATGTCCTTTGACACCGG+AGG 0.698582 4.2:+27531080 MS.gene031478:CDS
GTTCCGGAACAACCAATGTG+AGG 0.701296 4.2:-27529789 None:intergenic
GCAGCCGGTGAGATTTATGG+TGG 0.721413 4.2:+27531159 MS.gene031478:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTTTTTGGAAATTTA+GGG - chr4.2:27531713-27531732 None:intergenic 10.0%
!!! TATATTTTTTTTGGAAATTT+AGG - chr4.2:27531714-27531733 None:intergenic 10.0%
!!! TTTAACTTTAAAAGTAATTA+TGG - chr4.2:27528672-27528691 None:intergenic 10.0%
!! ATTTAGCAATAATAGTAATA+TGG + chr4.2:27528558-27528577 MS.gene031478:intron 15.0%
!! TGAAAAGATATACATAAAAA+AGG - chr4.2:27530811-27530830 None:intergenic 15.0%
!!! AAAGTTGTTTTTTTTTATTC+TGG + chr4.2:27529903-27529922 MS.gene031478:intron 15.0%
!!! AATTTTCCTTTAAATATGAT+AGG + chr4.2:27529251-27529270 MS.gene031478:intron 15.0%
!!! GGAAAAGAATTTATTTTTTT+TGG + chr4.2:27528844-27528863 MS.gene031478:intron 15.0%
!!! TAAATGGATTTATTGATTTT+TGG - chr4.2:27529100-27529119 None:intergenic 15.0%
!! AACATAAGTAAGTGTAATAT+TGG - chr4.2:27529411-27529430 None:intergenic 20.0%
!! ACATAAGTAAGTGTAATATT+GGG - chr4.2:27529410-27529429 None:intergenic 20.0%
!! ATATAATAGGAATAAAGAAG+TGG - chr4.2:27529694-27529713 None:intergenic 20.0%
!! ATATGTACTACTGAAAATAA+GGG - chr4.2:27530441-27530460 None:intergenic 20.0%
!! ATGGATTTAATGTATATTCA+CGG + chr4.2:27528577-27528596 MS.gene031478:intron 20.0%
!! ATTGAAGCTTATCATATAAT+AGG - chr4.2:27529707-27529726 None:intergenic 20.0%
!! GTCAATACTATAAACAAAAA+GGG - chr4.2:27531254-27531273 None:intergenic 20.0%
!! TATATGTACTACTGAAAATA+AGG - chr4.2:27530442-27530461 None:intergenic 20.0%
!! TCAATACTATAAACAAAAAG+GGG - chr4.2:27531253-27531272 None:intergenic 20.0%
!! TCTAATAAAAACACAATGTT+AGG - chr4.2:27528764-27528783 None:intergenic 20.0%
!! TGTCAATACTATAAACAAAA+AGG - chr4.2:27531255-27531274 None:intergenic 20.0%
!! TTATGTATAATACATCAATC+AGG + chr4.2:27530127-27530146 MS.gene031478:intron 20.0%
!! TTGCTAAATTAAATCATATG+TGG - chr4.2:27528547-27528566 None:intergenic 20.0%
!!! AAAAGTACAAGTTTATGATA+TGG + chr4.2:27530295-27530314 MS.gene031478:intron 20.0%
!!! AAAATAAATTCTTTTCCCAA+AGG - chr4.2:27528841-27528860 None:intergenic 20.0%
!!! AAAGTACCTATCATATTTAA+AGG - chr4.2:27529260-27529279 None:intergenic 20.0%
!!! AAATAAATTCTTTTCCCAAA+GGG - chr4.2:27528840-27528859 None:intergenic 20.0%
!!! AACATTGTGTTTTTATTAGA+TGG + chr4.2:27528764-27528783 MS.gene031478:intron 20.0%
!!! CAACAATAGTTTAAAATAAG+TGG - chr4.2:27529176-27529195 None:intergenic 20.0%
!!! TAGTATTTTAGCAACTAAAA+TGG + chr4.2:27530326-27530345 MS.gene031478:intron 20.0%
!!! TTTACTTTAGGTTAAAAAAG+AGG + chr4.2:27531475-27531494 MS.gene031478:intron 20.0%
!!! TTTTAAAAAGTCCATCTTAT+TGG + chr4.2:27531765-27531784 MS.gene031478:CDS 20.0%
! AAAAAGTTTACTAGGAAAAG+TGG + chr4.2:27530008-27530027 MS.gene031478:CDS 25.0%
! ACAAAATACTTATACGTAGA+TGG + chr4.2:27529484-27529503 MS.gene031478:intron 25.0%
! AGTAAATCCAGTAAAGAAAT+TGG - chr4.2:27528326-27528345 None:intergenic 25.0%
! ATCTAATATAGAAGCTATTC+TGG + chr4.2:27531674-27531693 MS.gene031478:intron 25.0%
! ATTCCACTTTACTTTACTTT+AGG + chr4.2:27531463-27531482 MS.gene031478:intron 25.0%
! CAAAATACTTATACGTAGAT+GGG + chr4.2:27529485-27529504 MS.gene031478:intron 25.0%
! CAATGTTTAATTACCAGATT+TGG + chr4.2:27529723-27529742 MS.gene031478:intron 25.0%
! CATAACAAATGATATACCTA+CGG - chr4.2:27529840-27529859 None:intergenic 25.0%
! CATAAGTAAGTGTAATATTG+GGG - chr4.2:27529409-27529428 None:intergenic 25.0%
! GATGGTCAATATCAATTAAT+TGG - chr4.2:27528956-27528975 None:intergenic 25.0%
! GCAACACAAATTATAAAAGA+AGG - chr4.2:27530846-27530865 None:intergenic 25.0%
! TAACCTAAAGTAAAGTAAAG+TGG - chr4.2:27531469-27531488 None:intergenic 25.0%
! TAAGTTTCTGTAGATCAATT+GGG - chr4.2:27530568-27530587 None:intergenic 25.0%
! TGGTTAATGATTCTTTGATA+TGG + chr4.2:27530189-27530208 MS.gene031478:intron 25.0%
! TTAAAGTTTGATACAGTGAT+TGG + chr4.2:27530169-27530188 MS.gene031478:intron 25.0%
!! AACTTGATAAGAAATCCTTT+TGG + chr4.2:27529809-27529828 MS.gene031478:CDS 25.0%
!! ACTTGATAAGAAATCCTTTT+GGG + chr4.2:27529810-27529829 MS.gene031478:CDS 25.0%
!! ACTTTTCCTAGTAAACTTTT+TGG - chr4.2:27530009-27530028 None:intergenic 25.0%
!! CAAACATTTTTAATGATCTG+AGG - chr4.2:27528642-27528661 None:intergenic 25.0%
!! CTTTTCCTAGTAAACTTTTT+GGG - chr4.2:27530008-27530027 None:intergenic 25.0%
!! TATATCTTTTCATGAGCAAT+AGG + chr4.2:27530819-27530838 MS.gene031478:intron 25.0%
!! TTAGGTTAAAAAAGAGGTAT+TGG + chr4.2:27531481-27531500 MS.gene031478:intron 25.0%
!! TTTAGAGTTATTAGCAGATA+TGG + chr4.2:27530229-27530248 MS.gene031478:intron 25.0%
!!! AACAATGATAAAGTGCTTTT+TGG - chr4.2:27530381-27530400 None:intergenic 25.0%
!!! ATATTGACCATCATGTTTTT+TGG + chr4.2:27528964-27528983 MS.gene031478:intron 25.0%
!!! ATGCAAGATAGTTTTACATA+AGG + chr4.2:27529446-27529465 MS.gene031478:intron 25.0%
!!! GATTTTTGGTTAGAATCTAA+TGG - chr4.2:27529086-27529105 None:intergenic 25.0%
!!! GTCAAATGAAAAGTATTACT+TGG + chr4.2:27530064-27530083 MS.gene031478:CDS 25.0%
!!! TAAGATGGACTTTTTAAAAC+CGG - chr4.2:27531764-27531783 None:intergenic 25.0%
!!! TACTTGGCTGTTATTTTTTA+CGG - chr4.2:27529030-27529049 None:intergenic 25.0%
!!! TGCAAGATAGTTTTACATAA+GGG + chr4.2:27529447-27529466 MS.gene031478:intron 25.0%
!!! TTTTCTGCTTGTTATATAGT+AGG + chr4.2:27528867-27528886 MS.gene031478:intron 25.0%
AAATTCTACTGAACAGCTAA+AGG - chr4.2:27529236-27529255 None:intergenic 30.0%
AAGGATTTCTTATCAAGTTC+CGG - chr4.2:27529808-27529827 None:intergenic 30.0%
AATCATTGTAGACAAACCAA+AGG + chr4.2:27531909-27531928 MS.gene031478:CDS 30.0%
ACAAATATAACATCTCCTTG+AGG - chr4.2:27528379-27528398 None:intergenic 30.0%
AGACTTCACTCTAAACTAAA+TGG - chr4.2:27529116-27529135 None:intergenic 30.0%
ATACTATAAACAAAAAGGGG+AGG - chr4.2:27531250-27531269 None:intergenic 30.0%
CAATTGATCTACAGAAACTT+AGG + chr4.2:27530567-27530586 MS.gene031478:CDS 30.0%
CTAAGTTTCTGTAGATCAAT+TGG - chr4.2:27530569-27530588 None:intergenic 30.0%
CTTGAGTAAATATATACCTG+CGG - chr4.2:27531640-27531659 None:intergenic 30.0%
GAATAAACCAAACACGAAAA+CGG - chr4.2:27528234-27528253 None:intergenic 30.0%
GCAGGAAATTATACTAGTAA+TGG + chr4.2:27529597-27529616 MS.gene031478:intron 30.0%
GGGAGGATACAAATTATAAA+TGG - chr4.2:27531339-27531358 None:intergenic 30.0%
GTACAAGTTTATGATATGGA+TGG + chr4.2:27530299-27530318 MS.gene031478:intron 30.0%
GTATATATCACACAACTCAA+CGG + chr4.2:27528350-27528369 MS.gene031478:CDS 30.0%
TAATAGGAATAAAGAAGTGG+TGG - chr4.2:27529691-27529710 None:intergenic 30.0%
TACTACTGAAAATAAGGGAA+GGG - chr4.2:27530436-27530455 None:intergenic 30.0%
TACTATAAACAAAAAGGGGA+GGG - chr4.2:27531249-27531268 None:intergenic 30.0%
TATATACAAGGACTCATACA+GGG - chr4.2:27529327-27529346 None:intergenic 30.0%
TGATGGGGATATTATATACA+AGG - chr4.2:27529339-27529358 None:intergenic 30.0%
TTAGGATGAAGAAATGCATA+AGG - chr4.2:27531418-27531437 None:intergenic 30.0%
TTATATACAAGGACTCATAC+AGG - chr4.2:27529328-27529347 None:intergenic 30.0%
TTGCTTGATATGCATCAAAA+TGG + chr4.2:27531808-27531827 MS.gene031478:CDS 30.0%
! AATGGTGAACTCACAATTTT+TGG - chr4.2:27531219-27531238 None:intergenic 30.0%
! AGATAGAAAAACTAACTTGC+AGG - chr4.2:27528424-27528443 None:intergenic 30.0%
! TAAAAAAGGCATAGCAAAAC+AGG - chr4.2:27530797-27530816 None:intergenic 30.0%
! TATGTGTTGATAAACCCTTT+GGG + chr4.2:27528823-27528842 MS.gene031478:intron 30.0%
!! AATGTTTTTGTTTGCTTGAG+TGG + chr4.2:27528465-27528484 MS.gene031478:intron 30.0%
!! GTTGCATGATTTGTTGAATT+TGG + chr4.2:27530861-27530880 MS.gene031478:intron 30.0%
!!! AATAAGTGGATCCACTTTTT+TGG - chr4.2:27529162-27529181 None:intergenic 30.0%
!!! ATAAGTGGATCCACTTTTTT+GGG - chr4.2:27529161-27529180 None:intergenic 30.0%
!!! GTTTTTTTTTATTCTGGCAG+TGG + chr4.2:27529909-27529928 MS.gene031478:intron 30.0%
AAAAGTCCATCTTATTGGAC+CGG + chr4.2:27531770-27531789 MS.gene031478:CDS 35.0%
AACAAGCAGTAACCATGATT+AGG - chr4.2:27531436-27531455 None:intergenic 35.0%
AATATAACATCTCCTTGAGG+AGG - chr4.2:27528376-27528395 None:intergenic 35.0%
ACAATGATTCGACGATAAGT+CGG - chr4.2:27531898-27531917 None:intergenic 35.0%
ACCTTTATTCCTACCTATGA+TGG + chr4.2:27531587-27531606 MS.gene031478:CDS 35.0%
ACTACTGAAAATAAGGGAAG+GGG - chr4.2:27530435-27530454 None:intergenic 35.0%
AGGAAATAGTGTCTGAAGAA+AGG + chr4.2:27531046-27531065 MS.gene031478:CDS 35.0%
AGGAAGTCCAAAAAACATGA+TGG - chr4.2:27528974-27528993 None:intergenic 35.0%
ATCATGCTTAGCTGAATGTT+GGG + chr4.2:27528792-27528811 MS.gene031478:intron 35.0%
ATGGAAGTTAGATCATCAGA+GGG + chr4.2:27528151-27528170 MS.gene031478:CDS 35.0%
ATTTGCTTTCTGTTAAGTGC+TGG - chr4.2:27529368-27529387 None:intergenic 35.0%
ATTTGTGTGAAGAAACAGGT+AGG + chr4.2:27528897-27528916 MS.gene031478:intron 35.0%
CAAAGCAAATCATCTCTAAC+AGG - chr4.2:27528994-27529013 None:intergenic 35.0%
CCAAAATCATTCATCACAGA+TGG + chr4.2:27531529-27531548 MS.gene031478:CDS 35.0%
CCTTTATTCCTACCTATGAT+GGG + chr4.2:27531588-27531607 MS.gene031478:CDS 35.0%
CTTACTTGAGTAGAGTTATG+AGG - chr4.2:27532006-27532025 None:intergenic 35.0%
GAGTTCCCAAAAAGTTTACT+AGG + chr4.2:27530000-27530019 MS.gene031478:CDS 35.0%
GCATTTCTTCATCCTAATCA+TGG + chr4.2:27531421-27531440 MS.gene031478:intron 35.0%
GGGGTACACTAAAAAGATTA+GGG - chr4.2:27529141-27529160 None:intergenic 35.0%
GTAATGATCTGACAAATGAG+AGG + chr4.2:27530956-27530975 MS.gene031478:intron 35.0%
GTACTACTGAAAATAAGGGA+AGG - chr4.2:27530437-27530456 None:intergenic 35.0%
GTAGATTTGTGTGAAGAAAC+AGG + chr4.2:27528893-27528912 MS.gene031478:intron 35.0%
TAAGTGCCTGGTAATTAACA+CGG - chr4.2:27530595-27530614 None:intergenic 35.0%
TACAAACTCATTCCATGGAA+GGG - chr4.2:27531836-27531855 None:intergenic 35.0%
TAGAAATAATCCTCCTCCTT+TGG - chr4.2:27531928-27531947 None:intergenic 35.0%
TGGCATTCCAATTTCTTTAC+TGG + chr4.2:27528316-27528335 MS.gene031478:CDS 35.0%
TGGGGTACACTAAAAAGATT+AGG - chr4.2:27529142-27529161 None:intergenic 35.0%
TTTAGTGTACCCCAAAAAAG+TGG + chr4.2:27529148-27529167 MS.gene031478:intron 35.0%
! ATTGCATCTGAGATATGAGA+TGG + chr4.2:27530688-27530707 MS.gene031478:CDS 35.0%
! CAAATCTGAAATGCCAAATC+TGG - chr4.2:27529739-27529758 None:intergenic 35.0%
! CCATCTGTGATGAATGATTT+TGG - chr4.2:27531532-27531551 None:intergenic 35.0%
! GTATGTGTTGATAAACCCTT+TGG + chr4.2:27528822-27528841 MS.gene031478:intron 35.0%
! TAAAGAAGTGGTGGAAGAAT+AGG - chr4.2:27529682-27529701 None:intergenic 35.0%
! TAACTTGCAGGAAGTAGAAT+GGG - chr4.2:27528412-27528431 None:intergenic 35.0%
! TACTTATACGTAGATGGGTT+TGG + chr4.2:27529490-27529509 MS.gene031478:intron 35.0%
! TATCTTATTAGCTCTCATGC+AGG + chr4.2:27531734-27531753 MS.gene031478:intron 35.0%
! TTTTCATTTGACCAGCTAAC+AGG - chr4.2:27530056-27530075 None:intergenic 35.0%
!! AATCCAAGTGCTGAAAAATG+GGG - chr4.2:27531359-27531378 None:intergenic 35.0%
!! ACAAAGTGTGATGATTTGCT+AGG + chr4.2:27531979-27531998 MS.gene031478:CDS 35.0%
!! ATTTGCTTTGTTGCGTTTTG+TGG + chr4.2:27529004-27529023 MS.gene031478:intron 35.0%
!! CAATCCAAGTGCTGAAAAAT+GGG - chr4.2:27531360-27531379 None:intergenic 35.0%
!! TCAATCCAAGTGCTGAAAAA+TGG - chr4.2:27531361-27531380 None:intergenic 35.0%
!! TGAAAAGTATTACTTGGAGC+AGG + chr4.2:27530070-27530089 MS.gene031478:CDS 35.0%
!!! TAAGTGGATCCACTTTTTTG+GGG - chr4.2:27529160-27529179 None:intergenic 35.0%
!!! TGATATGGCTTTTAAGACAG+CGG + chr4.2:27530204-27530223 MS.gene031478:intron 35.0%
AAAGGGGAGGGAGATATAAA+TGG - chr4.2:27531237-27531256 None:intergenic 40.0%
AACATGGCAAAACTTGTGGA+AGG + chr4.2:27531611-27531630 MS.gene031478:CDS 40.0%
ACACAGATTTACATTGCAGC+CGG + chr4.2:27531144-27531163 MS.gene031478:CDS 40.0%
ACAGGTTCACGAAAAGAGAA+TGG + chr4.2:27531867-27531886 MS.gene031478:CDS 40.0%
ACCCCTCAAATTGAGAATCT+GGG + chr4.2:27530620-27530639 MS.gene031478:CDS 40.0%
ACCTTGTGTTTGGCAAAAAG+CGG - chr4.2:27530500-27530519 None:intergenic 40.0%
AGCACTTGGATTGAAAGTTG+TGG + chr4.2:27531367-27531386 MS.gene031478:intron 40.0%
ATCAAAATGGAACCCTTCCA+TGG + chr4.2:27531821-27531840 MS.gene031478:CDS 40.0%
ATCTATCCCTACTCAGTACT+TGG - chr4.2:27529046-27529065 None:intergenic 40.0%
ATTTCCTTCTCTCTCCATGA+AGG - chr4.2:27531033-27531052 None:intergenic 40.0%
CAATGTAAATCTGTGTCTCC+CGG - chr4.2:27531140-27531159 None:intergenic 40.0%
CACAAGCTAGAATCATTCAG+TGG - chr4.2:27531301-27531320 None:intergenic 40.0%
CAGGTTCACGAAAAGAGAAT+GGG + chr4.2:27531868-27531887 MS.gene031478:CDS 40.0%
CATTGTAGACAAACCAAAGG+AGG + chr4.2:27531912-27531931 MS.gene031478:CDS 40.0%
CCCATCATAGGTAGGAATAA+AGG - chr4.2:27531591-27531610 None:intergenic 40.0%
CTACAAACTCATTCCATGGA+AGG - chr4.2:27531837-27531856 None:intergenic 40.0%
CTACTGAAAATAAGGGAAGG+GGG - chr4.2:27530434-27530453 None:intergenic 40.0%
CTCATACAGGGTCATTGAAA+TGG - chr4.2:27529315-27529334 None:intergenic 40.0%
GAACTGCTGTAACTCATCAA+GGG - chr4.2:27531510-27531529 None:intergenic 40.0%
GAGTAAATATATACCTGCGG+TGG - chr4.2:27531637-27531656 None:intergenic 40.0%
GATCATGCTTAGCTGAATGT+TGG + chr4.2:27528791-27528810 MS.gene031478:intron 40.0%
GCATCTACAAACTCATTCCA+TGG - chr4.2:27531841-27531860 None:intergenic 40.0%
GGCATTTCAGATTTGTGACA+TGG + chr4.2:27529744-27529763 MS.gene031478:intron 40.0%
GGTTTGGAAATGTGTAGCTT+TGG + chr4.2:27528499-27528518 MS.gene031478:intron 40.0%
TACGTAGCTTAACCCAAAGT+TGG + chr4.2:27530353-27530372 MS.gene031478:intron 40.0%
TACTGAAAATAAGGGAAGGG+GGG - chr4.2:27530433-27530452 None:intergenic 40.0%
TCAGATATGCATTCCCTTCA+TGG + chr4.2:27531016-27531035 MS.gene031478:intron 40.0%
TCATGCTTAGCTGAATGTTG+GGG + chr4.2:27528793-27528812 MS.gene031478:intron 40.0%
TCTGAAGAAAGGAGATCACA+GGG + chr4.2:27531057-27531076 MS.gene031478:CDS 40.0%
TGCGTTGGATTTCATGGTTT+CGG + chr4.2:27531553-27531572 MS.gene031478:CDS 40.0%
TTCACCACCATAAATCTCAC+CGG - chr4.2:27531166-27531185 None:intergenic 40.0%
TTGGGAGACCATTATTTGCT+AGG - chr4.2:27530550-27530569 None:intergenic 40.0%
TTGTGACTGTGAGTGAACTA+TGG + chr4.2:27528296-27528315 MS.gene031478:CDS 40.0%
TTTCCTTCTCTCTCCATGAA+GGG - chr4.2:27531032-27531051 None:intergenic 40.0%
TTTGTTCAACCATTGTGCTG+TGG + chr4.2:27528257-27528276 MS.gene031478:CDS 40.0%
! AGAATAGGAAGTACAAACCG+CGG - chr4.2:27529667-27529686 None:intergenic 40.0%
! CTAACTTGCAGGAAGTAGAA+TGG - chr4.2:27528413-27528432 None:intergenic 40.0%
! GCAATTGTACAAGCCTTTTC+CGG - chr4.2:27531792-27531811 None:intergenic 40.0%
! TTTTGCCATGTTCCCATCAT+AGG - chr4.2:27531603-27531622 None:intergenic 40.0%
!! ATAACAGCCAAGTACTGAGT+AGG + chr4.2:27529036-27529055 MS.gene031478:intron 40.0%
!! GAAAGTTGTGGCTCTGAAAA+TGG + chr4.2:27531379-27531398 MS.gene031478:intron 40.0%
!! TAACAGCCAAGTACTGAGTA+GGG + chr4.2:27529037-27529056 MS.gene031478:intron 40.0%
!! TAAGTGCTGGAGAGATTGAT+GGG - chr4.2:27529355-27529374 None:intergenic 40.0%
!! TGCTTTTTGGCACCAACTTT+GGG - chr4.2:27530368-27530387 None:intergenic 40.0%
!! TTAAGTGCTGGAGAGATTGA+TGG - chr4.2:27529356-27529375 None:intergenic 40.0%
!! TTCTAAGAGTGTCCTGCAAT+GGG + chr4.2:27529622-27529641 MS.gene031478:CDS 40.0%
!! TTTCTAAGAGTGTCCTGCAA+TGG + chr4.2:27529621-27529640 MS.gene031478:CDS 40.0%
!!! AGTGGTTTTGTGATGTGGTT+TGG + chr4.2:27528483-27528502 MS.gene031478:intron 40.0%
AAACGGCTCCTTGGAATCAA+AGG - chr4.2:27528217-27528236 None:intergenic 45.0%
AAGCTGCTCCTTTGATTCCA+AGG + chr4.2:27528206-27528225 MS.gene031478:CDS 45.0%
ACCTAAGGCTTGCATAACCA+AGG - chr4.2:27531112-27531131 None:intergenic 45.0%
ATTCAGATACTTCGCGAGAG+GGG + chr4.2:27530653-27530672 MS.gene031478:CDS 45.0%
ATTCCCTTCATGGAGAGAGA+AGG + chr4.2:27531026-27531045 MS.gene031478:CDS 45.0%
ATTGCAGCCGGTGAGATTTA+TGG + chr4.2:27531156-27531175 MS.gene031478:CDS 45.0%
CAAACATGTCCACAGCACAA+TGG - chr4.2:27528269-27528288 None:intergenic 45.0%
CACACTTTGTTCCCTCACAA+AGG - chr4.2:27531969-27531988 None:intergenic 45.0%
CACCCCTCAAATTGAGAATC+TGG + chr4.2:27530619-27530638 MS.gene031478:CDS 45.0%
CAGCTAAAGGAGTTGTACAG+TGG - chr4.2:27529223-27529242 None:intergenic 45.0%
CATTGTGCTGTGGACATGTT+TGG + chr4.2:27528267-27528286 MS.gene031478:CDS 45.0%
CCATCTTATTGGACCGGAAA+AGG + chr4.2:27531776-27531795 MS.gene031478:CDS 45.0%
CCCCAGATTCTCAATTTGAG+GGG - chr4.2:27530624-27530643 None:intergenic 45.0%
CCCCTCAAATTGAGAATCTG+GGG + chr4.2:27530621-27530640 MS.gene031478:CDS 45.0%
CTCAGATGCAATGCCACAAA+AGG - chr4.2:27530680-27530699 None:intergenic 45.0%
CTGCAAGATTTCAGCTGACA+AGG - chr4.2:27529581-27529600 None:intergenic 45.0%
CTTGTCAGCTGAAATCTTGC+AGG + chr4.2:27529579-27529598 MS.gene031478:intron 45.0%
GAGATATGAGATGGACATGC+TGG + chr4.2:27530697-27530716 MS.gene031478:CDS 45.0%
GAGTTGTACAGTGGAGAATC+TGG - chr4.2:27529214-27529233 None:intergenic 45.0%
GATGCACGCCTAGCAAATAA+TGG + chr4.2:27530539-27530558 MS.gene031478:CDS 45.0%
GATTCAGATACTTCGCGAGA+GGG + chr4.2:27530652-27530671 MS.gene031478:CDS 45.0%
GATTTCATGGTTTCGGTAGC+CGG + chr4.2:27531560-27531579 MS.gene031478:CDS 45.0%
GCCCCAGATTCTCAATTTGA+GGG - chr4.2:27530625-27530644 None:intergenic 45.0%
GGAACTGCTGTAACTCATCA+AGG - chr4.2:27531511-27531530 None:intergenic 45.0%
GTACAGTGGAGAATCTGGAT+GGG - chr4.2:27529209-27529228 None:intergenic 45.0%
GTCTGAAGAAAGGAGATCAC+AGG + chr4.2:27531056-27531075 MS.gene031478:CDS 45.0%
GTGACATGGTGACAATTGCT+CGG + chr4.2:27529758-27529777 MS.gene031478:CDS 45.0%
TAAGGGAAGGGGGGAAATAT+AGG - chr4.2:27530424-27530443 None:intergenic 45.0%
TAGCTCTCATGCAGGTATTC+CGG + chr4.2:27531742-27531761 MS.gene031478:intron 45.0%
TCATTTGACCAGCTAACAGG+AGG - chr4.2:27530053-27530072 None:intergenic 45.0%
TGATTCAGATACTTCGCGAG+AGG + chr4.2:27530651-27530670 MS.gene031478:CDS 45.0%
TGCCCCAGATTCTCAATTTG+AGG - chr4.2:27530626-27530645 None:intergenic 45.0%
TGGGAACATGGCAAAACTTG+TGG + chr4.2:27531607-27531626 MS.gene031478:CDS 45.0%
TGTACAGTGGAGAATCTGGA+TGG - chr4.2:27529210-27529229 None:intergenic 45.0%
TGTAGACAAACCAAAGGAGG+AGG + chr4.2:27531915-27531934 MS.gene031478:CDS 45.0%
TTGATTCAAACCCCCATTGC+AGG - chr4.2:27529637-27529656 None:intergenic 45.0%
TTGCACTAATGAAGAAGCCG+AGG + chr4.2:27530742-27530761 MS.gene031478:CDS 45.0%
TTTGAATCAAATGCGTGCCG+CGG + chr4.2:27529647-27529666 MS.gene031478:CDS 45.0%
! ACGTAGATGGGTTTGGTAGA+CGG + chr4.2:27529497-27529516 MS.gene031478:intron 45.0%
! CCTTTTCCGGTCCAATAAGA+TGG - chr4.2:27531779-27531798 None:intergenic 45.0%
! GAACCTCACATTGGTTGTTC+CGG + chr4.2:27529786-27529805 MS.gene031478:CDS 45.0%
! GCTTTCTCAGGTTGTACTCT+TGG + chr4.2:27530719-27530738 MS.gene031478:CDS 45.0%
! GCTTTTTGCCAAACACAAGG+TGG + chr4.2:27530499-27530518 MS.gene031478:CDS 45.0%
! TAGGTGCCGTGTTAATTACC+AGG + chr4.2:27530586-27530605 MS.gene031478:CDS 45.0%
! TCCTACCTATGATGGGAACA+TGG + chr4.2:27531595-27531614 MS.gene031478:CDS 45.0%
! TCGGCTTTTGAACCTCACAT+TGG + chr4.2:27529777-27529796 MS.gene031478:CDS 45.0%
!! AAGTGCTGGAGAGATTGATG+GGG - chr4.2:27529354-27529373 None:intergenic 45.0%
!! GGTAGGAATAAAGGTGTCAC+CGG - chr4.2:27531582-27531601 None:intergenic 45.0%
!! GTGCTTTTTGGCACCAACTT+TGG - chr4.2:27530369-27530388 None:intergenic 45.0%
!! GTTGTGGCTCTGAAAATGGT+TGG + chr4.2:27531383-27531402 MS.gene031478:intron 45.0%
!! TCTAAGAGTGTCCTGCAATG+GGG + chr4.2:27529623-27529642 MS.gene031478:CDS 45.0%
!! TGCAAGCCTTAGGTTTTGAC+CGG + chr4.2:27531118-27531137 MS.gene031478:CDS 45.0%
!!! ACCGCTTTTTGCCAAACACA+AGG + chr4.2:27530496-27530515 MS.gene031478:CDS 45.0%
!!! GCTTGAGTGGTTTTGTGATG+TGG + chr4.2:27528478-27528497 MS.gene031478:intron 45.0%
!!! AATAAGATATATATTTTTTT+TGG - chr4.2:27531723-27531742 None:intergenic 5.0%
AAGAAGCCGAGGATCTCAAG+CGG + chr4.2:27530753-27530772 MS.gene031478:CDS 50.0%
ATAACCAAGGCAGTCTCCTC+CGG - chr4.2:27531099-27531118 None:intergenic 50.0%
ATACCTACGGTCTGCCCAAA+AGG - chr4.2:27529827-27529846 None:intergenic 50.0%
CAAACACGAAAACGGCTCCT+TGG - chr4.2:27528226-27528245 None:intergenic 50.0%
CCTCATGAGTGCCTTTGTGA+GGG + chr4.2:27531955-27531974 MS.gene031478:CDS 50.0%
CTTGCAGGAAGTAGAATGGG+AGG - chr4.2:27528409-27528428 None:intergenic 50.0%
GAAAAGTGGATACTCGACCC+TGG + chr4.2:27530022-27530041 MS.gene031478:CDS 50.0%
GAAGTGCACCACCTTGTGTT+TGG - chr4.2:27530510-27530529 None:intergenic 50.0%
GAGGGGTGAATTTAAGTGCC+TGG - chr4.2:27530607-27530626 None:intergenic 50.0%
GATGGCTGCGTTGGATTTCA+TGG + chr4.2:27531547-27531566 MS.gene031478:CDS 50.0%
GCCATGTTCCCATCATAGGT+AGG - chr4.2:27531599-27531618 None:intergenic 50.0%
GCCTTGGTTATGCAAGCCTT+AGG + chr4.2:27531108-27531127 MS.gene031478:CDS 50.0%
GGGTCTATGTCCTTTGACAC+CGG + chr4.2:27531077-27531096 MS.gene031478:CDS 50.0%
GTTCCGGAACAACCAATGTG+AGG - chr4.2:27529792-27529811 None:intergenic 50.0%
GTTTGTAGATGCTGTGCGAC+AGG + chr4.2:27531849-27531868 MS.gene031478:CDS 50.0%
TCTATGTCCTTTGACACCGG+AGG + chr4.2:27531080-27531099 MS.gene031478:CDS 50.0%
TGTCTCCCGGTCAAAACCTA+AGG - chr4.2:27531127-27531146 None:intergenic 50.0%
TTATTCTGGCAGTGGCTTCG+AGG + chr4.2:27529917-27529936 MS.gene031478:intron 50.0%
TTCATCACAGATGGCTGCGT+TGG + chr4.2:27531538-27531557 MS.gene031478:CDS 50.0%
! AATCCTTTTGGGCAGACCGT+AGG + chr4.2:27529821-27529840 MS.gene031478:CDS 50.0%
! CGTAGATGGGTTTGGTAGAC+GGG + chr4.2:27529498-27529517 MS.gene031478:intron 50.0%
! GTAGATGGGTTTGGTAGACG+GGG + chr4.2:27529499-27529518 MS.gene031478:intron 50.0%
!! ACAAAGGCACTCATGAGGGT+TGG - chr4.2:27531953-27531972 None:intergenic 50.0%
!! ATGGACATGCTGGCTTTCTC+AGG + chr4.2:27530707-27530726 MS.gene031478:CDS 50.0%
!! CAAGGAGCCGTTTTCGTGTT+TGG + chr4.2:27528224-27528243 MS.gene031478:CDS 50.0%
!! CCAAGTGCTGAAAAATGGGG+AGG - chr4.2:27531356-27531375 None:intergenic 50.0%
!! CCTCACAAAGGCACTCATGA+GGG - chr4.2:27531957-27531976 None:intergenic 50.0%
!! CCTCCCCATTTTTCAGCACT+TGG + chr4.2:27531353-27531372 MS.gene031478:intron 50.0%
!! CTAAGAGTGTCCTGCAATGG+GGG + chr4.2:27529624-27529643 MS.gene031478:CDS 50.0%
!! GCAAGCCTTAGGTTTTGACC+GGG + chr4.2:27531119-27531138 MS.gene031478:CDS 50.0%
ACAACTCAACGGCCTCCTCA+AGG + chr4.2:27528361-27528380 MS.gene031478:CDS 55.0%
CCCTCATGAGTGCCTTTGTG+AGG + chr4.2:27531954-27531973 MS.gene031478:CDS 55.0%
CCTCATCCGCTTGAGATCCT+CGG - chr4.2:27530762-27530781 None:intergenic 55.0%
GCAGCCGGTGAGATTTATGG+TGG + chr4.2:27531159-27531178 MS.gene031478:CDS 55.0%
TGGAGATGCCTCCTGTTAGC+TGG + chr4.2:27530042-27530061 MS.gene031478:CDS 55.0%
!! AGCTAACAGGAGGCATCTCC+AGG - chr4.2:27530043-27530062 None:intergenic 55.0%
!! CCCTCACAAAGGCACTCATG+AGG - chr4.2:27531958-27531977 None:intergenic 55.0%
!! GCTAACAGGAGGCATCTCCA+GGG - chr4.2:27530042-27530061 None:intergenic 55.0%
AACTTGTGGAAGGCCACCGC+AGG + chr4.2:27531621-27531640 MS.gene031478:CDS 60.0%
CCGAGGATCTCAAGCGGATG+AGG + chr4.2:27530759-27530778 MS.gene031478:CDS 60.0%
! GCAGTCTCCTCCGGTGTCAA+AGG - chr4.2:27531090-27531109 None:intergenic 60.0%
! TCGCGAGAGGGGACCTTTTG+TGG + chr4.2:27530664-27530683 MS.gene031478:CDS 60.0%
GACACCGGAGGAGACTGCCT+TGG + chr4.2:27531092-27531111 MS.gene031478:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 27528151 27532029 27528151 ID=MS.gene031478
chr4.2 mRNA 27528151 27532029 27528151 ID=MS.gene031478.t1;Parent=MS.gene031478
chr4.2 exon 27528151 27528427 27528151 ID=MS.gene031478.t1.exon1;Parent=MS.gene031478.t1
chr4.2 CDS 27528151 27528427 27528151 ID=cds.MS.gene031478.t1;Parent=MS.gene031478.t1
chr4.2 exon 27529601 27529668 27529601 ID=MS.gene031478.t1.exon2;Parent=MS.gene031478.t1
chr4.2 CDS 27529601 27529668 27529601 ID=cds.MS.gene031478.t1;Parent=MS.gene031478.t1
chr4.2 exon 27529754 27529842 27529754 ID=MS.gene031478.t1.exon3;Parent=MS.gene031478.t1
chr4.2 CDS 27529754 27529842 27529754 ID=cds.MS.gene031478.t1;Parent=MS.gene031478.t1
chr4.2 exon 27529929 27530091 27529929 ID=MS.gene031478.t1.exon4;Parent=MS.gene031478.t1
chr4.2 CDS 27529929 27530091 27529929 ID=cds.MS.gene031478.t1;Parent=MS.gene031478.t1
chr4.2 exon 27530491 27530780 27530491 ID=MS.gene031478.t1.exon5;Parent=MS.gene031478.t1
chr4.2 CDS 27530491 27530780 27530491 ID=cds.MS.gene031478.t1;Parent=MS.gene031478.t1
chr4.2 exon 27531020 27531224 27531020 ID=MS.gene031478.t1.exon6;Parent=MS.gene031478.t1
chr4.2 CDS 27531020 27531224 27531020 ID=cds.MS.gene031478.t1;Parent=MS.gene031478.t1
chr4.2 exon 27531485 27531642 27531485 ID=MS.gene031478.t1.exon7;Parent=MS.gene031478.t1
chr4.2 CDS 27531485 27531642 27531485 ID=cds.MS.gene031478.t1;Parent=MS.gene031478.t1
chr4.2 exon 27531756 27532029 27531756 ID=MS.gene031478.t1.exon8;Parent=MS.gene031478.t1
chr4.2 CDS 27531756 27532029 27531756 ID=cds.MS.gene031478.t1;Parent=MS.gene031478.t1
Gene Sequence

>MS.gene031478

ATGGAAGTTAGATCATCAGAGGGTAATCAAGTGAGATATTATGATAAGCTTCTTCAAGCTGCTCCTTTGATTCCAAGGAGCCGTTTTCGTGTTTGGTTTATTCGAATTTGTTCAACCATTGTGCTGTGGACATGTTTGGTTCAGCTTGTGACTGTGAGTGAACTATGGCATTCCAATTTCTTTACTGGATTTACTAGTCGTATATATCACACAACTCAACGGCCTCCTCAAGGAGATGTTATATTTGTTCGATCTCCTCCCATTCTACTTCCTGCAAGTTAGTTTTTCTATCTACTTACTTATCTCAAGATTGCAATGTTTTTGTTTGCTTGAGTGGTTTTGTGATGTGGTTTGGAAATGTGTAGCTTTGGTTGAATCTATGATGCAAACTCTCCACATATGATTTAATTTAGCAATAATAGTAATATGGATTTAATGTATATTCACGGTTAGTGTAAAGATTTTTTACACTGCGAATCAATCATTATCCTCAGATCATTAAAAATGTTTGACTTCAACCATAATTACTTTTAAAGTTAAACATATGATTAGTAGTGTTTTGTTTTGACAATATATCAAAATTCAAATATCATAATATTTATATGATCAACCTAACATTGTGTTTTTATTAGATGGTAATGATCATGCTTAGCTGAATGTTGGGGCATAATGTATGTGTTGATAAACCCTTTGGGAAAAGAATTTATTTTTTTTGGTTTTCTGCTTGTTATATAGTAGGTTTGTAGATTTGTGTGAAGAAACAGGTAGGAGCATGAATGTTAGTGACTTACAATTGTTAATACCAATTAATTGATATTGACCATCATGTTTTTTGGACTTCCTGTTAGAGATGATTTGCTTTGTTGCGTTTTGTGGCCGTAAAAAATAACAGCCAAGTACTGAGTAGGGATAGATACATAGATCAGTTGATGCCATTAGATTCTAACCAAAAATCAATAAATCCATTTAGTTTAGAGTGAAGTCTTTCCCTAATCTTTTTAGTGTACCCCAAAAAAGTGGATCCACTTATTTTAAACTATTGTTGATACTTAATGCCCATCCAGATTCTCCACTGTACAACTCCTTTAGCTGTTCAGTAGAATTTTCCTTTAAATATGATAGGTACTTTTTTTTATCTCAAATTATGTTTGAAGCATTTGTCCATTTCAATGACCCTGTATGAGTCCTTGTATATAATATCCCCATCAATCTCTCCAGCACTTAACAGAAAGCAAATAAGAGTCTAACTGAAGATCCCCAATATTACACTTACTTATGTTGAGACATGCATATGCATGCAAGATAGTTTTACATAAGGGTATAAGTAAAAAAAACAAAATACTTATACGTAGATGGGTTTGGTAGACGGGGTATGAGTATCATTAGCTGTTTATATCTTTTCTGATGCACTTTTGTAAATATTAAAGCCTTGTCAGCTGAAATCTTGCAGGAAATTATACTAGTAATGGTTTTCTAAGAGTGTCCTGCAATGGGGGTTTGAATCAAATGCGTGCCGCGGTTTGTACTTCCTATTCTTCCACCACTTCTTTATTCCTATTATATGATAAGCTTCAATGTTTAATTACCAGATTTGGCATTTCAGATTTGTGACATGGTGACAATTGCTCGGCTTTTGAACCTCACATTGGTTGTTCCGGAACTTGATAAGAAATCCTTTTGGGCAGACCGTAGGTATATCATTTGTTATGAAAAAAAAGAGTATTCTCTAATTTGCTTGTGTTGTGTCATGTTAAAGTTGTTTTTTTTTATTCTGGCAGTGGCTTCGAGGATATATTTGATGTAAGACATTTCATTGATTCTTTACGAGATGAAGTTCGAATAGTGAAAAGAGTTCCCAAAAAGTTTACTAGGAAAAGTGGATACTCGACCCTGGAGATGCCTCCTGTTAGCTGGTCAAATGAAAAGTATTACTTGGAGCAGGTCTGCAGATTCTATTTGTTGCACTTAATCTAAAATTATGTATAATACATCAATCAGGTTTTCTCTTATTTGACAATTTAAAGTTTGATACAGTGATTGGTTAATGATTCTTTGATATGGCTTTTAAGACAGCGGCATTTAGAGTTATTAGCAGATATGGAAACATTTTATAATATCTTGAAATATTAATCATTCATATGAAGAAAAGTACAAGTTTATGATATGGATGGCTTCTAGTATTTTAGCAACTAAAATGGATTGTACGTAGCTTAACCCAAAGTTGGTGCCAAAAAGCACTTTATCATTGTTGAAGTGAAAAACTGTAGTTACCTATATTTCCCCCCTTCCCTTATTTTCAGTAGTACATATATTGAGTTTGACTATCGACTCTAACTGCAGATTCTACCGCTTTTTGCCAAACACAAGGTGGTGCACTTCAACAAAACAGATGCACGCCTAGCAAATAATGGTCTCCCAATTGATCTACAGAAACTTAGGTGCCGTGTTAATTACCAGGCACTTAAATTCACCCCTCAAATTGAGAATCTGGGGCAAAAATTGATTCAGATACTTCGCGAGAGGGGACCTTTTGTGGCATTGCATCTGAGATATGAGATGGACATGCTGGCTTTCTCAGGTTGTACTCTTGGTTGCACTAATGAAGAAGCCGAGGATCTCAAGCGGATGAGGTATTTTATGTTTCCTGTTTTGCTATGCCTTTTTTATGTATATCTTTTCATGAGCAATAGGTCCTTCTTTTATAATTTGTGTTGCATGATTTGTTGAATTTGGCATATTTTGACAACTACAAATTAAGTACATTCATTTTATAAAAGATTAAGAGTCTTGTGTTCAAAAGAATTTGTAATGATCTGACAAATGAGAGGTCTATCTAAGATAAAATTCTGATTTTGCTTGCATTATTCAGATATGCATTCCCTTCATGGAGAGAGAAGGAAATAGTGTCTGAAGAAAGGAGATCACAGGGTCTATGTCCTTTGACACCGGAGGAGACTGCCTTGGTTATGCAAGCCTTAGGTTTTGACCGGGAGACACAGATTTACATTGCAGCCGGTGAGATTTATGGTGGTGAACGTAGACTTGCGCAACTGAGAGCTGCATTTCCAAAAATTGTGAGTTCACCATTTATATCTCCCTCCCCTTTTTGTTTATAGTATTGACATTGACATTTGATGTAATAAAGTACCACTGAATGATTCTAGCTTGTGCTTATGCACGATTTTCCATTTATAATTTGTATCCTCCCCATTTTTCAGCACTTGGATTGAAAGTTGTGGCTCTGAAAATGGTTGGCTTAATCTACCTTATGCATTTCTTCATCCTAATCATGGTTACTGCTTGTTTGATAAGATTCCACTTTACTTTACTTTAGGTTAAAAAAGAGGTATTGGTCGCCCTTGATGAGTTACAGCAGTTCCAAAATCATTCATCACAGATGGCTGCGTTGGATTTCATGGTTTCGGTAGCCGGTGACACCTTTATTCCTACCTATGATGGGAACATGGCAAAACTTGTGGAAGGCCACCGCAGGTATATATTTACTCAAGCTAGTTGCAATTTCATCTAATATAGAAGCTATTCTGGTTTTAAAAAACATCCCTAAATTTCCAAAAAAAATATATATCTTATTAGCTCTCATGCAGGTATTCCGGTTTTAAAAAGTCCATCTTATTGGACCGGAAAAGGCTTGTACAATTGCTTGATATGCATCAAAATGGAACCCTTCCATGGAATGAGTTTGTAGATGCTGTGCGACAGGTTCACGAAAAGAGAATGGGTCAGCCGACTTATCGTCGAATCATTGTAGACAAACCAAAGGAGGAGGATTATTTCTACGCCAACCCTCATGAGTGCCTTTGTGAGGGAACAAAGTGTGATGATTTGCTAGGTCCTCATAACTCTACTCAAGTAAGATGA
Protein sequence

>MS.gene031478.t1

MEVRSSEGNQVRYYDKLLQAAPLIPRSRFRVWFIRICSTIVLWTCLVQLVTVSELWHSNFFTGFTSRIYHTTQRPPQGDVIFVRSPPILLPARNYTSNGFLRVSCNGGLNQMRAAICDMVTIARLLNLTLVVPELDKKSFWADRSGFEDIFDVRHFIDSLRDEVRIVKRVPKKFTRKSGYSTLEMPPVSWSNEKYYLEQILPLFAKHKVVHFNKTDARLANNGLPIDLQKLRCRVNYQALKFTPQIENLGQKLIQILRERGPFVALHLRYEMDMLAFSGCTLGCTNEEAEDLKRMRYAFPSWREKEIVSEERRSQGLCPLTPEETALVMQALGFDRETQIYIAAGEIYGGERRLAQLRAAFPKIVKKEVLVALDELQQFQNHSSQMAALDFMVSVAGDTFIPTYDGNMAKLVEGHRRYSGFKKSILLDRKRLVQLLDMHQNGTLPWNEFVDAVRQVHEKRMGQPTYRRIIVDKPKEEDYFYANPHECLCEGTKCDDLLGPHNSTQVR