AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96774

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96774.t1 MTR_4g082310 98.422 507 8 0 1 507 1 507 0.0 1046
MS.gene96774.t1 MTR_5g042200 80.041 486 90 3 28 506 9 494 0.0 823
MS.gene96774.t1 MTR_6g016015 59.006 483 196 2 8 488 25 507 0.0 605
MS.gene96774.t1 MTR_4g110030 61.588 466 173 2 29 488 43 508 0.0 603
MS.gene96774.t1 MTR_2g059350 57.809 493 202 3 8 499 30 517 0.0 597
MS.gene96774.t1 MTR_5g064620 48.675 415 201 4 87 489 97 511 2.45e-140 414
MS.gene96774.t1 MTR_4g125460 48.485 396 197 5 97 490 89 479 2.58e-137 405
MS.gene96774.t1 MTR_4g125460 48.485 396 197 5 97 490 228 618 1.18e-135 406
MS.gene96774.t1 MTR_4g019210 48.371 399 199 5 97 493 232 625 4.40e-134 402
MS.gene96774.t1 MTR_3g099480 47.952 415 201 5 87 489 126 537 1.82e-131 392
MS.gene96774.t1 MTR_5g081690 46.599 397 205 5 96 490 120 511 5.29e-130 388
MS.gene96774.t1 MTR_3g096020 45.735 422 200 8 87 489 175 586 1.23e-123 374
MS.gene96774.t1 MTR_3g115220 43.358 399 207 9 98 492 112 495 7.80e-100 312
MS.gene96774.t1 MTR_4g100810 40.876 411 220 8 93 497 104 497 4.23e-98 306
MS.gene96774.t1 MTR_5g099010 40.393 458 239 11 54 505 90 519 1.94e-97 306
MS.gene96774.t1 MTR_5g012770 41.304 414 209 11 92 499 119 504 9.32e-92 289
MS.gene96774.t1 MTR_5g032100 38.051 431 218 9 97 491 86 503 1.49e-90 286
MS.gene96774.t1 MTR_3g086570 39.487 390 216 7 90 464 144 528 2.96e-89 284
MS.gene96774.t1 MTR_3g100310 36.613 437 228 9 91 491 94 517 7.84e-88 279
MS.gene96774.t1 MTR_1g102030 37.939 427 233 11 83 492 74 485 7.76e-85 271
MS.gene96774.t1 MTR_4g084570 35.096 416 243 10 97 496 150 554 7.47e-82 265
MS.gene96774.t1 MTR_4g132590 37.642 441 220 11 94 489 62 492 1.04e-79 257
MS.gene96774.t1 MTR_5g059260 36.650 412 231 10 98 489 158 559 9.18e-79 258
MS.gene96774.t1 MTR_4g132590 37.642 441 220 11 94 489 161 591 1.90e-78 257
MS.gene96774.t1 MTR_2g016470 35.892 443 231 10 91 490 183 615 4.24e-78 256
MS.gene96774.t1 MTR_1g031590 34.223 412 244 9 97 492 149 549 1.51e-76 251
MS.gene96774.t1 MTR_3g005340 35.507 414 232 11 97 490 147 545 3.35e-74 245
MS.gene96774.t1 MTR_7g064210 71.687 166 37 2 212 368 24 188 1.41e-73 239
MS.gene96774.t1 MTR_3g026650 36.432 398 234 9 100 492 176 559 1.36e-71 238
MS.gene96774.t1 MTR_4g023030 36.409 401 236 8 100 495 236 622 8.36e-71 237
MS.gene96774.t1 MTR_3g086570 39.519 291 160 5 90 366 144 432 1.47e-66 221
MS.gene96774.t1 MTR_1g101990 39.249 293 158 9 206 492 3 281 2.03e-60 201
MS.gene96774.t1 MTR_3g026650 38.245 319 185 6 100 413 176 487 7.80e-60 205
MS.gene96774.t1 MTR_4g010990 33.957 374 221 9 90 457 68 421 1.20e-58 201
MS.gene96774.t1 MTR_4g010990 32.670 352 211 9 112 457 1 332 3.61e-50 176
MS.gene96774.t1 MTR_3g092590 32.686 309 180 8 94 379 82 385 1.61e-42 159
MS.gene96774.t1 MTR_3g092590 32.686 309 180 8 94 379 82 385 9.73e-42 158
MS.gene96774.t1 MTR_7g017330 31.148 305 185 7 85 370 86 384 4.46e-39 151
MS.gene96774.t1 MTR_3g026650 38.528 231 130 6 100 325 176 399 3.13e-37 142
MS.gene96774.t1 MTR_1g007390 30.159 315 186 9 94 379 82 391 7.90e-34 135
MS.gene96774.t1 MTR_1g101985 52.747 91 43 0 83 173 74 164 3.68e-25 102
MS.gene96774.t1 MTR_4g008200 26.608 342 201 13 97 427 35 337 4.95e-24 103
MS.gene96774.t1 MTR_4g008200 26.225 347 196 12 97 427 86 388 1.16e-23 103
MS.gene96774.t1 MTR_1g052225 37.195 164 53 7 95 258 19 132 2.01e-18 82.0
MS.gene96774.t1 MTR_6g046230 38.318 107 58 2 145 243 34 140 4.56e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96774.t1 AT1G14020 71.881 505 136 2 1 505 1 499 0.0 761
MS.gene96774.t1 AT2G03280 69.528 466 130 4 24 488 26 480 0.0 687
MS.gene96774.t1 AT2G03280 65.720 493 130 5 24 488 26 507 0.0 672
MS.gene96774.t1 AT5G15740 58.030 467 194 2 23 488 33 498 0.0 581
MS.gene96774.t1 AT3G02250 58.491 477 190 4 15 488 27 498 0.0 576
MS.gene96774.t1 AT2G37980 46.453 437 227 5 73 507 202 633 1.31e-137 411
MS.gene96774.t1 AT3G54100 46.065 432 216 7 73 497 198 619 1.17e-133 401
MS.gene96774.t1 AT5G35570 47.407 405 206 5 96 498 250 649 2.10e-132 399
MS.gene96774.t1 AT1G22460 45.370 432 216 6 76 490 130 558 2.45e-129 388
MS.gene96774.t1 AT5G64600 46.778 419 200 8 87 489 103 514 1.72e-128 384
MS.gene96774.t1 AT5G01100 47.132 401 204 5 97 493 216 612 2.85e-128 387
MS.gene96774.t1 AT1G76270 44.584 397 204 7 100 494 109 491 1.93e-104 324
MS.gene96774.t1 AT4G38390 43.500 400 212 7 97 494 123 510 1.97e-102 318
MS.gene96774.t1 AT1G20550 42.157 408 220 7 100 505 112 505 6.38e-102 317
MS.gene96774.t1 AT4G16650 43.521 409 207 10 89 490 113 504 3.26e-95 299
MS.gene96774.t1 AT4G16650 43.521 409 207 10 89 490 113 504 3.26e-95 299
MS.gene96774.t1 AT1G04910 36.853 464 259 10 50 506 57 493 7.37e-92 290
MS.gene96774.t1 AT4G24530 37.674 430 219 9 97 490 101 517 9.50e-90 284
MS.gene96774.t1 AT3G26370 38.974 390 218 7 90 464 152 536 3.28e-89 284
MS.gene96774.t1 AT5G65470 35.581 430 226 9 99 490 87 503 1.53e-86 275
MS.gene96774.t1 AT1G11990 39.173 411 222 10 98 491 191 590 2.11e-82 267
MS.gene96774.t1 AT1G62330 34.862 436 232 8 98 489 222 649 2.71e-82 268
MS.gene96774.t1 AT1G38065 36.427 431 238 11 79 489 24 438 5.64e-82 263
MS.gene96774.t1 AT1G38065 37.046 413 230 10 97 489 26 428 6.50e-82 262
MS.gene96774.t1 AT1G38131 37.046 413 230 10 97 489 155 557 1.69e-80 262
MS.gene96774.t1 AT1G11990 36.161 448 221 10 98 491 176 612 1.79e-77 254
MS.gene96774.t1 AT5G63390 38.000 400 228 9 100 493 167 552 1.88e-76 251
MS.gene96774.t1 AT1G29200 37.931 435 219 11 97 490 70 494 2.59e-76 248
MS.gene96774.t1 AT1G29200 37.931 435 219 11 97 490 70 494 2.59e-76 248
MS.gene96774.t1 AT1G35510 35.012 417 240 10 92 490 154 557 3.87e-75 248
MS.gene96774.t1 AT1G29200 37.931 435 219 11 97 490 186 610 4.81e-75 248
MS.gene96774.t1 AT2G01480 36.314 369 213 7 97 449 154 516 1.08e-74 246
MS.gene96774.t1 AT1G14970 34.140 413 245 10 97 493 9 410 1.32e-74 242
MS.gene96774.t1 AT1G14970 34.140 413 245 10 97 493 35 436 2.46e-74 242
MS.gene96774.t1 AT2G01480 36.695 357 207 6 97 437 154 507 2.89e-74 244
MS.gene96774.t1 AT1G29200 37.762 429 216 11 103 490 2 420 3.82e-74 241
MS.gene96774.t1 AT1G14970 34.140 413 245 10 97 493 155 556 2.98e-73 242
MS.gene96774.t1 AT2G44500 37.626 396 227 10 100 489 180 561 6.14e-73 242
MS.gene96774.t1 AT3G07900 34.634 410 247 8 84 489 180 572 5.71e-69 231
MS.gene96774.t1 AT1G52630 32.708 373 224 10 90 458 70 419 5.07e-59 201
MS.gene96774.t1 AT3G03810 35.855 304 178 7 91 379 83 384 8.37e-50 181
MS.gene96774.t1 AT3G03810 35.855 304 178 7 91 379 83 384 8.37e-50 181
MS.gene96774.t1 AT1G52630 31.054 351 218 9 112 458 1 331 1.07e-49 174
MS.gene96774.t1 AT3G26370 39.171 217 119 5 90 296 152 365 1.09e-45 164
MS.gene96774.t1 AT3G30300 33.333 306 180 7 91 379 97 395 2.87e-43 163
MS.gene96774.t1 AT3G30300 33.333 306 180 7 91 379 97 395 2.87e-43 163
MS.gene96774.t1 AT2G44500 39.914 233 127 7 100 326 180 405 4.49e-39 147
MS.gene96774.t1 AT1G51630 24.845 322 192 9 96 406 90 372 7.29e-19 89.4

Find 131 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCTGTGATGAATGATTT+TGG 0.203102 4.3:-28125768 None:intergenic
ATTGCAGCCGGTGAGATTTA+TGG 0.227102 4.3:+28125394 MS.gene96774:CDS
TGGCATTCCAATTTCTTTAC+TGG 0.232585 4.3:+28122565 MS.gene96774:CDS
TCAGATATGCATTCCCTTCA+TGG 0.261168 4.3:+28125254 MS.gene96774:intron
GATGGCTGCTTTGGATTTCA+TGG 0.269401 4.3:+28125786 MS.gene96774:CDS
AGTAAATCCAGTAAAGAAAT+TGG 0.278555 4.3:-28122572 None:intergenic
CACCCCTCAAATTGAGAATC+TGG 0.293410 4.3:+28124857 MS.gene96774:CDS
TGCTTTGGATTTCATGGTTT+CGG 0.294885 4.3:+28125792 MS.gene96774:CDS
TTCATCACAGATGGCTGCTT+TGG 0.305983 4.3:+28125777 MS.gene96774:CDS
TTGCTTGATATGCATCAAAA+TGG 0.313563 4.3:+28126047 MS.gene96774:CDS
CTAAGTTTCTGTAGATCAAT+TGG 0.331955 4.3:-28124804 None:intergenic
TTCTAAGAGTGTCCTGCAAC+GGG 0.335100 4.3:+28123861 MS.gene96774:CDS
AAGGATTTCTTATCAAGTTC+CGG 0.337356 4.3:-28124044 None:intergenic
GCTTTCTCAGGTTGTACTCT+TGG 0.343079 4.3:+28124957 MS.gene96774:CDS
AACTTGATAAGAAATCCTTT+TGG 0.353716 4.3:+28124048 MS.gene96774:CDS
TAGAAATAATCCTCCTCCTT+TGG 0.360561 4.3:-28126164 None:intergenic
TAGCGCTCATGCAGGTATTC+CGG 0.363730 4.3:+28125981 MS.gene96774:intron
CATTGTGCTGTGGACATGTT+TGG 0.367522 4.3:+28122516 MS.gene96774:CDS
ACCTTTATTCCTACCTATGA+TGG 0.388352 4.3:+28125826 MS.gene96774:CDS
AGCTAACAGGAGGCATCTCC+AGG 0.388940 4.3:-28124279 None:intergenic
ATGGACATGCTGGCTTTCTC+AGG 0.395187 4.3:+28124945 MS.gene96774:CDS
TGCCCCAGATTCTCAATTTG+AGG 0.396144 4.3:-28124861 None:intergenic
GAACCTCACATTGGTTGTTC+CGG 0.404001 4.3:+28124025 MS.gene96774:CDS
CCATCTTATTGGACCGGAAA+AGG 0.405140 4.3:+28126015 MS.gene96774:CDS
GCCTTGGTTATGCAAGCCTT+AGG 0.432969 4.3:+28125346 MS.gene96774:CDS
CCCATCATAGGTAGGAATAA+AGG 0.436770 4.3:-28125827 None:intergenic
GCAGGAAATTATACTAGTAA+TGG 0.439060 4.3:+28123836 MS.gene96774:intron
ATACCTACGGTCTGCCCAAA+AGG 0.445579 4.3:-28124063 None:intergenic
AGGAAATAGTGTCTGAAGAA+AGG 0.445827 4.3:+28125284 MS.gene96774:CDS
GATTTCATGGTTTCGGTAGC+CGG 0.450519 4.3:+28125799 MS.gene96774:CDS
GGAACTGCTGTAACTCATCA+AGG 0.452325 4.3:-28125747 None:intergenic
AAAAGTCCATCTTATTGGAC+CGG 0.458534 4.3:+28126009 MS.gene96774:CDS
GAAGTGCACCACCTTGTGTT+TGG 0.463947 4.3:-28124745 None:intergenic
AAGCTGCTCCTTTGATTCCA+AGG 0.468221 4.3:+28122455 MS.gene96774:CDS
CTTACTTGAGTAGAGTTATG+AGG 0.468781 4.3:-28126242 None:intergenic
TTGGGAGACCATTATTTGCT+AGG 0.469220 4.3:-28124785 None:intergenic
GCCCCAGATTCTCAATTTGA+GGG 0.469715 4.3:-28124860 None:intergenic
TTATTCTGGCAGTGGCTTCG+AGG 0.470272 4.3:+28124156 MS.gene96774:intron
GAATAAACCAAACACGAAAA+CGG 0.470528 4.3:-28122480 None:intergenic
CTAACTTGCAGGAAGTAGAA+TGG 0.473374 4.3:-28122659 None:intergenic
TAAGTTTCTGTAGATCAATT+GGG 0.475386 4.3:-28124803 None:intergenic
TGGAGATGCCTCCTGTTAGC+TGG 0.475776 4.3:+28124281 MS.gene96774:CDS
TTGTGACTGTGAGTGAACTA+TGG 0.476628 4.3:+28122545 MS.gene96774:CDS
TAACTTGCAGGAAGTAGAAT+GGG 0.479064 4.3:-28122658 None:intergenic
CTAAGAGTGTCCTGCAACGG+GGG 0.480371 4.3:+28123863 MS.gene96774:CDS
CAAACACGAAAACGGCTCCT+TGG 0.481546 4.3:-28122472 None:intergenic
ACCCCTCAAATTGAGAATCT+GGG 0.484445 4.3:+28124858 MS.gene96774:CDS
GAGGGGTGAATTTAAGTGCC+TGG 0.487600 4.3:-28124842 None:intergenic
GTCAAATGAAAAGTATTACT+TGG 0.489028 4.3:+28124303 MS.gene96774:CDS
CTCAGATGCAATGCCACAAA+AGG 0.490594 4.3:-28124915 None:intergenic
TTTCTAAGAGTGTCCTGCAA+CGG 0.498838 4.3:+28123860 MS.gene96774:CDS
ATTTCCTTCTCTCTCCATGA+AGG 0.500444 4.3:-28125268 None:intergenic
GATGCACGCCTAGCAAATAA+TGG 0.504541 4.3:+28124777 MS.gene96774:CDS
ATAACCAAGGCAGTCTCCTC+CGG 0.505709 4.3:-28125334 None:intergenic
CAATTGATCTACAGAAACTT+AGG 0.505738 4.3:+28124805 MS.gene96774:CDS
ACAGGTTCACGAAAAGAGAA+TGG 0.506682 4.3:+28126106 MS.gene96774:CDS
CCGAGGATCTCAAGCGGATG+AGG 0.509548 4.3:+28124997 MS.gene96774:CDS
GTCTGAAGAAAGGAGATCCC+AGG 0.512801 4.3:+28125294 MS.gene96774:CDS
GCAGTCTCCTCCGGTGTCAA+AGG 0.517244 4.3:-28125325 None:intergenic
GTGTCAAAGGACATAGACCC+TGG 0.517880 4.3:-28125312 None:intergenic
GACACCGGAGGAGACTGCCT+TGG 0.525025 4.3:+28125330 MS.gene96774:CDS
GCATCTACAAACTCATTCCA+TGG 0.525256 4.3:-28126077 None:intergenic
CCTCATCCGCTTGAGATCCT+CGG 0.534275 4.3:-28124997 None:intergenic
GTTTGTAGATGCTGTGCGAC+AGG 0.534296 4.3:+28126088 MS.gene96774:CDS
CCTTTATTCCTACCTATGAT+GGG 0.535492 4.3:+28125827 MS.gene96774:CDS
TGAAAAGTATTACTTGGAGC+AGG 0.539964 4.3:+28124309 MS.gene96774:CDS
CCAAAATCATTCATCACAGA+TGG 0.542359 4.3:+28125768 MS.gene96774:CDS
TGTCTCCCGGTCAAAACCTA+AGG 0.543702 4.3:-28125362 None:intergenic
CTACAAACTCATTCCATGGA+AGG 0.551044 4.3:-28126073 None:intergenic
AAACGGCTCCTTGGAATCAA+AGG 0.552528 4.3:-28122463 None:intergenic
CCCCAGATTCTCAATTTGAG+GGG 0.555415 4.3:-28124859 None:intergenic
GAGATATGAGATGGACATGC+TGG 0.557518 4.3:+28124935 MS.gene96774:CDS
CAGGTTCACGAAAAGAGAAT+GGG 0.560835 4.3:+28126107 MS.gene96774:CDS
ATCAAAATGGAACCCTTCCA+TGG 0.561331 4.3:+28126060 MS.gene96774:CDS
CAATGTAAATCTGTGTCTCC+CGG 0.564211 4.3:-28125375 None:intergenic
TTTCCTTCTCTCTCCATGAA+GGG 0.565721 4.3:-28125267 None:intergenic
CCCTCATGAGTGCCTTTGTG+AGG 0.571802 4.3:+28126193 MS.gene96774:CDS
ACACAGATTTACATTGCAGC+CGG 0.575721 4.3:+28125382 MS.gene96774:CDS
GGGTCTATGTCCTTTGACAC+CGG 0.576261 4.3:+28125315 MS.gene96774:CDS
ACAATGATTCGACGATAAGT+CGG 0.577569 4.3:-28126134 None:intergenic
GTGACATGGTGACAATTGCT+CGG 0.579350 4.3:+28123997 MS.gene96774:CDS
TGGGAACATGGCAAAACTTG+TGG 0.580054 4.3:+28125846 MS.gene96774:CDS
TAGGTGCCGTGTTAATTACC+AGG 0.582283 4.3:+28124824 MS.gene96774:CDS
GATTCAGATACTTCGCGAGA+GGG 0.583952 4.3:+28124890 MS.gene96774:CDS
TGTAGACAAACCAAAGGAGG+AGG 0.587728 4.3:+28126154 MS.gene96774:CDS
TACAAACTCATTCCATGGAA+GGG 0.592326 4.3:-28126072 None:intergenic
GGTAGGAATAAAGGTGTCAC+CGG 0.594798 4.3:-28125818 None:intergenic
ACAAAGGCACTCATGAGGGT+TGG 0.596296 4.3:-28126189 None:intergenic
CCCTCACAAAGGCACTCATG+AGG 0.597403 4.3:-28126194 None:intergenic
CACACTTTGTTCCCTCACAA+AGG 0.600880 4.3:-28126205 None:intergenic
AACTTGTGGAAGGCCACCGC+AGG 0.602466 4.3:+28125860 MS.gene96774:CDS
CCTCACAAAGGCACTCATGA+GGG 0.602588 4.3:-28126193 None:intergenic
GCCATGTTCCCATCATAGGT+AGG 0.602990 4.3:-28125835 None:intergenic
CCCCTCAAATTGAGAATCTG+GGG 0.605300 4.3:+28124859 MS.gene96774:CDS
ACAACTCAACGGCCTCCTCA+AGG 0.607711 4.3:+28122610 MS.gene96774:CDS
AACATGGCAAAACTTGTGGA+AGG 0.609196 4.3:+28125850 MS.gene96774:CDS
CCTCATGAGTGCCTTTGTGA+GGG 0.609759 4.3:+28126194 MS.gene96774:CDS
TCCTACCTATGATGGGAACA+TGG 0.610654 4.3:+28125834 MS.gene96774:CDS
TTTGAATCAAATGCGTGCAG+CGG 0.612731 4.3:+28123886 MS.gene96774:CDS
ACAAAGTGTGATGATTTGCT+AGG 0.614058 4.3:+28126218 MS.gene96774:CDS
AATGGAAGTTAGATCATCAG+AGG 0.614061 4.3:+28122399 None:intergenic
GGCATTTCAGATTTGTGACA+TGG 0.614628 4.3:+28123983 MS.gene96774:intron
CATTGTAGACAAACCAAAGG+AGG 0.615228 4.3:+28126151 MS.gene96774:CDS
TTCACCACCATAAATCTCAC+CGG 0.615230 4.3:-28125401 None:intergenic
TCTAAGAGTGTCCTGCAACG+GGG 0.617187 4.3:+28123862 MS.gene96774:CDS
TCATTTGACCAGCTAACAGG+AGG 0.621119 4.3:-28124289 None:intergenic
ACCTAAGGCTTGCATAACCA+AGG 0.627242 4.3:-28125347 None:intergenic
GTATATATCACACAACTCAA+CGG 0.629814 4.3:+28122599 MS.gene96774:CDS
GAAAAGTGGATACTCGACCC+TGG 0.636896 4.3:+28124261 MS.gene96774:CDS
AATCATTGTAGACAAACCAA+AGG 0.637419 4.3:+28126148 MS.gene96774:CDS
ATTCAGATACTTCGCGAGAG+GGG 0.637675 4.3:+28124891 MS.gene96774:CDS
GAACTGCTGTAACTCATCAA+GGG 0.638982 4.3:-28125746 None:intergenic
TCTGAAGAAAGGAGATCCCA+GGG 0.644505 4.3:+28125295 MS.gene96774:CDS
TGTCAAAGGACATAGACCCT+GGG 0.644623 4.3:-28125311 None:intergenic
TTTGTTCAACCATTGTGCTG+TGG 0.645471 4.3:+28122506 MS.gene96774:CDS
TGATTCAGATACTTCGCGAG+AGG 0.649037 4.3:+28124889 MS.gene96774:CDS
ATTCCCTTCATGGAGAGAGA+AGG 0.650807 4.3:+28125264 MS.gene96774:CDS
CTTGCAGGAAGTAGAATGGG+AGG 0.651294 4.3:-28122655 None:intergenic
AATATAACATCTCCTTGAGG+AGG 0.657753 4.3:-28122622 None:intergenic
TAAGTGCCTGGTAATTAACA+CGG 0.657828 4.3:-28124830 None:intergenic
ATGGAAGTTAGATCATCAGA+GGG 0.666260 4.3:+28122400 MS.gene96774:CDS
TTGCACTAATGAAGAAGCCG+AGG 0.669813 4.3:+28124980 MS.gene96774:CDS
CAAACATGTCCACAGCACAA+TGG 0.677892 4.3:-28122515 None:intergenic
AAGAAGCCGAGGATCTCAAG+CGG 0.678663 4.3:+28124991 MS.gene96774:CDS
GCTAACAGGAGGCATCTCCA+GGG 0.682429 4.3:-28124278 None:intergenic
ATTGCATCTGAGATATGAGA+TGG 0.683390 4.3:+28124926 MS.gene96774:CDS
TCTATGTCCTTTGACACCGG+AGG 0.698582 4.3:+28125318 MS.gene96774:CDS
GCAAATATAACATCTCCTTG+AGG 0.698776 4.3:-28122625 None:intergenic
GTTCCGGAACAACCAATGTG+AGG 0.701296 4.3:-28124028 None:intergenic
GCAGCCGGTGAGATTTATGG+TGG 0.721413 4.3:+28125397 MS.gene96774:CDS
GAGTAAATAGATACCTGCGG+TGG 0.724695 4.3:-28125873 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAGATATATATTTCTTT+TGG - chr4.3:28125962-28125981 None:intergenic 10.0%
!!! TTTAACTTTAAAAGTAATTA+TGG - chr4.3:28122921-28122940 None:intergenic 10.0%
!! ATTTAGCAATAATAGTAATA+TGG + chr4.3:28122807-28122826 MS.gene96774:intron 15.0%
!! TGAAAAGATATACATAAAAA+AGG - chr4.3:28125049-28125068 None:intergenic 15.0%
!!! AAAGTTGTTTTTTTTTATTC+TGG + chr4.3:28124142-28124161 MS.gene96774:intron 15.0%
!!! AATTTTCCTTTAAATATGAT+AGG + chr4.3:28123500-28123519 MS.gene96774:intron 15.0%
!!! ATATTTCTTTTGGAAATTTA+GGG - chr4.3:28125952-28125971 None:intergenic 15.0%
!!! GGAAAAGAATTTATTTTTTT+TGG + chr4.3:28123093-28123112 MS.gene96774:intron 15.0%
!!! TAAATGGATTTATTGATTTT+TGG - chr4.3:28123349-28123368 None:intergenic 15.0%
!!! TATATTTCTTTTGGAAATTT+AGG - chr4.3:28125953-28125972 None:intergenic 15.0%
!! AACATAAGTAAGTGTAATAT+TGG - chr4.3:28123651-28123670 None:intergenic 20.0%
!! ACATAAGTAAGTGTAATATT+GGG - chr4.3:28123650-28123669 None:intergenic 20.0%
!! ATATAATAGGAATAAAGAAG+TGG - chr4.3:28123933-28123952 None:intergenic 20.0%
!! ATATGTACTACTGAAAATAA+GGG - chr4.3:28124679-28124698 None:intergenic 20.0%
!! ATGGATTTAATGTATATTCA+CGG + chr4.3:28122826-28122845 MS.gene96774:intron 20.0%
!! ATTGAAGCTTATCATATAAT+AGG - chr4.3:28123946-28123965 None:intergenic 20.0%
!! GTCAATACTATAAACAAAAA+GGG - chr4.3:28125493-28125512 None:intergenic 20.0%
!! TATATGTACTACTGAAAATA+AGG - chr4.3:28124680-28124699 None:intergenic 20.0%
!! TCAATACTATAAACAAAAAG+GGG - chr4.3:28125492-28125511 None:intergenic 20.0%
!! TCTAATAAAAACACAATGTT+AGG - chr4.3:28123013-28123032 None:intergenic 20.0%
!! TGTCAATACTATAAACAAAA+AGG - chr4.3:28125494-28125513 None:intergenic 20.0%
!! TTATGTATAATACATCAATC+AGG + chr4.3:28124366-28124385 MS.gene96774:intron 20.0%
!! TTGCTAAATTAAATCATATG+TGG - chr4.3:28122796-28122815 None:intergenic 20.0%
!!! AAAAGTACAAGTTTATGATA+TGG + chr4.3:28124534-28124553 MS.gene96774:intron 20.0%
!!! AAAATAAATTCTTTTCCCAA+AGG - chr4.3:28123090-28123109 None:intergenic 20.0%
!!! AAAGTACCTATCATATTTAA+AGG - chr4.3:28123509-28123528 None:intergenic 20.0%
!!! AAATAAATTCTTTTCCCAAA+GGG - chr4.3:28123089-28123108 None:intergenic 20.0%
!!! AACATTGTGTTTTTATTAGA+TGG + chr4.3:28123013-28123032 MS.gene96774:intron 20.0%
!!! CAACAATAGTTTAAAATAAG+TGG - chr4.3:28123425-28123444 None:intergenic 20.0%
!!! TAGTATTTTAGCAACTAAAA+TGG + chr4.3:28124565-28124584 MS.gene96774:intron 20.0%
!!! TTTACTTTAGGTTAAAAAAG+AGG + chr4.3:28125714-28125733 MS.gene96774:intron 20.0%
!!! TTTAGAGTTATTATCAGATA+TGG + chr4.3:28124468-28124487 MS.gene96774:intron 20.0%
!!! TTTTAAAAAGTCCATCTTAT+TGG + chr4.3:28126004-28126023 MS.gene96774:CDS 20.0%
! AAAAAGTTTACTAGGAAAAG+TGG + chr4.3:28124247-28124266 MS.gene96774:CDS 25.0%
! ACAAAATACTTATACGTAGA+TGG + chr4.3:28123723-28123742 MS.gene96774:intron 25.0%
! AGTAAATCCAGTAAAGAAAT+TGG - chr4.3:28122575-28122594 None:intergenic 25.0%
! ATCTAATATAGAAGCTATTC+TGG + chr4.3:28125913-28125932 MS.gene96774:intron 25.0%
! ATTCCACTTTACTTTACTTT+AGG + chr4.3:28125702-28125721 MS.gene96774:intron 25.0%
! CAATACTATAAACAAAAAGG+GGG - chr4.3:28125491-28125510 None:intergenic 25.0%
! CAATGTTTAATTACCAGATT+TGG + chr4.3:28123962-28123981 MS.gene96774:intron 25.0%
! CATAACAAATGATATACCTA+CGG - chr4.3:28124079-28124098 None:intergenic 25.0%
! CATAAGTAAGTGTAATATTG+GGG - chr4.3:28123649-28123668 None:intergenic 25.0%
! GATGGTCAATATCAATTAAT+TGG - chr4.3:28123205-28123224 None:intergenic 25.0%
! GCAACACAAATTATAAAAGA+AGG - chr4.3:28125084-28125103 None:intergenic 25.0%
! GTTTATCAACACATACATTA+TGG - chr4.3:28123067-28123086 None:intergenic 25.0%
! TAACCTAAAGTAAAGTAAAG+TGG - chr4.3:28125708-28125727 None:intergenic 25.0%
! TAAGTTTCTGTAGATCAATT+GGG - chr4.3:28124806-28124825 None:intergenic 25.0%
! TGGTTAATGATTCTTTGATA+TGG + chr4.3:28124428-28124447 MS.gene96774:intron 25.0%
! TTAAAGTTTGATACAGTGAT+TGG + chr4.3:28124408-28124427 MS.gene96774:intron 25.0%
!! AACTTGATAAGAAATCCTTT+TGG + chr4.3:28124048-28124067 MS.gene96774:CDS 25.0%
!! ACTTGATAAGAAATCCTTTT+GGG + chr4.3:28124049-28124068 MS.gene96774:CDS 25.0%
!! ACTTTTCCTAGTAAACTTTT+TGG - chr4.3:28124248-28124267 None:intergenic 25.0%
!! CAAACATTTTAAATGATCTG+AGG - chr4.3:28122891-28122910 None:intergenic 25.0%
!! CTTTTCCTAGTAAACTTTTT+GGG - chr4.3:28124247-28124266 None:intergenic 25.0%
!! TATATCTTTTCATGAGCAAT+AGG + chr4.3:28125057-28125076 MS.gene96774:intron 25.0%
!! TTAGGTTAAAAAAGAGGTAT+TGG + chr4.3:28125720-28125739 MS.gene96774:intron 25.0%
!!! AACATAGATAAAGTGCTTTT+TGG - chr4.3:28124620-28124639 None:intergenic 25.0%
!!! ATATTGACCATCATGTTTTT+TGG + chr4.3:28123213-28123232 MS.gene96774:intron 25.0%
!!! GATTTTTGGTTAGAATCTAA+TGG - chr4.3:28123335-28123354 None:intergenic 25.0%
!!! GTCAAATGAAAAGTATTACT+TGG + chr4.3:28124303-28124322 MS.gene96774:CDS 25.0%
!!! TAAGATGGACTTTTTAAAAC+CGG - chr4.3:28126003-28126022 None:intergenic 25.0%
!!! TACTTGGCTGTTATTTTTTA+CGG - chr4.3:28123279-28123298 None:intergenic 25.0%
!!! TTTTCTGCTTGTTATATAGT+AGG + chr4.3:28123116-28123135 MS.gene96774:intron 25.0%
AAATTCTACTGAACAGCTAA+AGG - chr4.3:28123485-28123504 None:intergenic 30.0%
AAGGATTTCTTATCAAGTTC+CGG - chr4.3:28124047-28124066 None:intergenic 30.0%
AATCATTGTAGACAAACCAA+AGG + chr4.3:28126148-28126167 MS.gene96774:CDS 30.0%
AGACTTCACTCTAAACTAAA+TGG - chr4.3:28123365-28123384 None:intergenic 30.0%
CAATTGATCTACAGAAACTT+AGG + chr4.3:28124805-28124824 MS.gene96774:CDS 30.0%
CTAAGTTTCTGTAGATCAAT+TGG - chr4.3:28124807-28124826 None:intergenic 30.0%
GAATAAACCAAACACGAAAA+CGG - chr4.3:28122483-28122502 None:intergenic 30.0%
GCAGGAAATTATACTAGTAA+TGG + chr4.3:28123836-28123855 MS.gene96774:intron 30.0%
GGGAGGATACAAATTATAAA+TGG - chr4.3:28125578-28125597 None:intergenic 30.0%
GTACAAGTTTATGATATGGA+TGG + chr4.3:28124538-28124557 MS.gene96774:intron 30.0%
GTATATATCACACAACTCAA+CGG + chr4.3:28122599-28122618 MS.gene96774:CDS 30.0%
TAATAGGAATAAAGAAGTGG+TGG - chr4.3:28123930-28123949 None:intergenic 30.0%
TACTACTGAAAATAAGGGAA+GGG - chr4.3:28124674-28124693 None:intergenic 30.0%
TATATACAAGGACTCATACA+GGG - chr4.3:28123574-28123593 None:intergenic 30.0%
TGATGGGGATATTATATACA+AGG - chr4.3:28123586-28123605 None:intergenic 30.0%
TTAGGATGAAGAAATGCATA+AGG - chr4.3:28125657-28125676 None:intergenic 30.0%
TTATATACAAGGACTCATAC+AGG - chr4.3:28123575-28123594 None:intergenic 30.0%
TTGCTTGATATGCATCAAAA+TGG + chr4.3:28126047-28126066 MS.gene96774:CDS 30.0%
! AATGGTGAACTCACAATTTT+TGG - chr4.3:28125457-28125476 None:intergenic 30.0%
! AGATAGAAAAACTAACTTGC+AGG - chr4.3:28122673-28122692 None:intergenic 30.0%
! ATTAAGAGTCTTGTGTTCAA+AGG + chr4.3:28125166-28125185 MS.gene96774:intron 30.0%
! TAAAAAAGGCATAGCAAAAC+AGG - chr4.3:28125035-28125054 None:intergenic 30.0%
! TACTTATACGTAGATGGATT+TGG + chr4.3:28123729-28123748 MS.gene96774:intron 30.0%
! TATGTGTTGATAAACCCTTT+GGG + chr4.3:28123072-28123091 MS.gene96774:intron 30.0%
!! AATGTTTTTGTTTGCTTGAG+TGG + chr4.3:28122714-28122733 MS.gene96774:intron 30.0%
!! GTTGCATGATTTGTTGAATT+TGG + chr4.3:28125099-28125118 MS.gene96774:intron 30.0%
!!! AATAAGTGGATCCACTTTTT+TGG - chr4.3:28123411-28123430 None:intergenic 30.0%
!!! ATAAGTGGATCCACTTTTTT+GGG - chr4.3:28123410-28123429 None:intergenic 30.0%
!!! GTTTTTTTTTATTCTGGCAG+TGG + chr4.3:28124148-28124167 MS.gene96774:intron 30.0%
AAAAGTCCATCTTATTGGAC+CGG + chr4.3:28126009-28126028 MS.gene96774:CDS 35.0%
AACAAGCAGTAACCATGATT+AGG - chr4.3:28125675-28125694 None:intergenic 35.0%
AATATAACATCTCCTTGAGG+AGG - chr4.3:28122625-28122644 None:intergenic 35.0%
ACAATGATTCGACGATAAGT+CGG - chr4.3:28126137-28126156 None:intergenic 35.0%
ACCTTTATTCCTACCTATGA+TGG + chr4.3:28125826-28125845 MS.gene96774:CDS 35.0%
ACTACTGAAAATAAGGGAAG+GGG - chr4.3:28124673-28124692 None:intergenic 35.0%
ACTATAAACAAAAAGGGGGA+GGG - chr4.3:28125487-28125506 None:intergenic 35.0%
AGGAAATAGTGTCTGAAGAA+AGG + chr4.3:28125284-28125303 MS.gene96774:CDS 35.0%
AGGAAGTCCAAAAAACATGA+TGG - chr4.3:28123223-28123242 None:intergenic 35.0%
ATCATGCTTAGCTGAATGTT+GGG + chr4.3:28123041-28123060 MS.gene96774:intron 35.0%
ATGGAAGTTAGATCATCAGA+GGG + chr4.3:28122400-28122419 MS.gene96774:CDS 35.0%
ATTTGTGTGAAGAAACAGGT+AGG + chr4.3:28123146-28123165 MS.gene96774:intron 35.0%
CAAAGCAAATCATCTCTAAC+AGG - chr4.3:28123243-28123262 None:intergenic 35.0%
CCAAAATCATTCATCACAGA+TGG + chr4.3:28125768-28125787 MS.gene96774:CDS 35.0%
CCTTTATTCCTACCTATGAT+GGG + chr4.3:28125827-28125846 MS.gene96774:CDS 35.0%
CTTACTTGAGTAGAGTTATG+AGG - chr4.3:28126245-28126264 None:intergenic 35.0%
GAGTTCCCAAAAAGTTTACT+AGG + chr4.3:28124239-28124258 MS.gene96774:CDS 35.0%
GCAAATATAACATCTCCTTG+AGG - chr4.3:28122628-28122647 None:intergenic 35.0%
GCATTTCTTCATCCTAATCA+TGG + chr4.3:28125660-28125679 MS.gene96774:intron 35.0%
GGGGTACACTAAAAAGATTA+GGG - chr4.3:28123390-28123409 None:intergenic 35.0%
GTACTACTGAAAATAAGGGA+AGG - chr4.3:28124675-28124694 None:intergenic 35.0%
GTAGATTTGTGTGAAGAAAC+AGG + chr4.3:28123142-28123161 MS.gene96774:intron 35.0%
TAAGTGCCTGGTAATTAACA+CGG - chr4.3:28124833-28124852 None:intergenic 35.0%
TACAAACTCATTCCATGGAA+GGG - chr4.3:28126075-28126094 None:intergenic 35.0%
TACTATAAACAAAAAGGGGG+AGG - chr4.3:28125488-28125507 None:intergenic 35.0%
TAGAAATAATCCTCCTCCTT+TGG - chr4.3:28126167-28126186 None:intergenic 35.0%
TGGCATTCCAATTTCTTTAC+TGG + chr4.3:28122565-28122584 MS.gene96774:CDS 35.0%
TGGGGTACACTAAAAAGATT+AGG - chr4.3:28123391-28123410 None:intergenic 35.0%
TTTAGTGTACCCCAAAAAAG+TGG + chr4.3:28123397-28123416 MS.gene96774:intron 35.0%
! ATTGCATCTGAGATATGAGA+TGG + chr4.3:28124926-28124945 MS.gene96774:CDS 35.0%
! CAAATCTGAAATGCCAAATC+TGG - chr4.3:28123978-28123997 None:intergenic 35.0%
! CCATCTGTGATGAATGATTT+TGG - chr4.3:28125771-28125790 None:intergenic 35.0%
! GTATGTGTTGATAAACCCTT+TGG + chr4.3:28123071-28123090 MS.gene96774:intron 35.0%
! TAAAGAAGTGGTGGAAGAAT+AGG - chr4.3:28123921-28123940 None:intergenic 35.0%
! TAACTTGCAGGAAGTAGAAT+GGG - chr4.3:28122661-28122680 None:intergenic 35.0%
! TGCTTTGGATTTCATGGTTT+CGG + chr4.3:28125792-28125811 MS.gene96774:CDS 35.0%
! TTTTCATTTGACCAGCTAAC+AGG - chr4.3:28124295-28124314 None:intergenic 35.0%
!! AATCCAAGTGCTGAAAAATG+GGG - chr4.3:28125598-28125617 None:intergenic 35.0%
!! ACAAAGTGTGATGATTTGCT+AGG + chr4.3:28126218-28126237 MS.gene96774:CDS 35.0%
!! ATTTGCTTTGTTGCGTTTTG+TGG + chr4.3:28123253-28123272 MS.gene96774:intron 35.0%
!! CAATCCAAGTGCTGAAAAAT+GGG - chr4.3:28125599-28125618 None:intergenic 35.0%
!! TCAATCCAAGTGCTGAAAAA+TGG - chr4.3:28125600-28125619 None:intergenic 35.0%
!! TGAAAAGTATTACTTGGAGC+AGG + chr4.3:28124309-28124328 MS.gene96774:CDS 35.0%
!!! TAAGTGGATCCACTTTTTTG+GGG - chr4.3:28123409-28123428 None:intergenic 35.0%
AACATGGCAAAACTTGTGGA+AGG + chr4.3:28125850-28125869 MS.gene96774:CDS 40.0%
ACACAGATTTACATTGCAGC+CGG + chr4.3:28125382-28125401 MS.gene96774:CDS 40.0%
ACAGGTTCACGAAAAGAGAA+TGG + chr4.3:28126106-28126125 MS.gene96774:CDS 40.0%
ACCCCTCAAATTGAGAATCT+GGG + chr4.3:28124858-28124877 MS.gene96774:CDS 40.0%
ACCTTGTGTTTGGCAAAAAG+CGG - chr4.3:28124738-28124757 None:intergenic 40.0%
AGCACTTGGATTGAAAGTTG+TGG + chr4.3:28125606-28125625 MS.gene96774:intron 40.0%
ATCAAAATGGAACCCTTCCA+TGG + chr4.3:28126060-28126079 MS.gene96774:CDS 40.0%
ATCTATCCCTACTCAGTACT+TGG - chr4.3:28123295-28123314 None:intergenic 40.0%
ATTTCCTTCTCTCTCCATGA+AGG - chr4.3:28125271-28125290 None:intergenic 40.0%
CAATGTAAATCTGTGTCTCC+CGG - chr4.3:28125378-28125397 None:intergenic 40.0%
CACAAGCTAGAATCATTCAG+TGG - chr4.3:28125540-28125559 None:intergenic 40.0%
CAGGTTCACGAAAAGAGAAT+GGG + chr4.3:28126107-28126126 MS.gene96774:CDS 40.0%
CATTGTAGACAAACCAAAGG+AGG + chr4.3:28126151-28126170 MS.gene96774:CDS 40.0%
CCCATCATAGGTAGGAATAA+AGG - chr4.3:28125830-28125849 None:intergenic 40.0%
CGTGAGTAAATAGATACCTG+CGG - chr4.3:28125879-28125898 None:intergenic 40.0%
CTACAAACTCATTCCATGGA+AGG - chr4.3:28126076-28126095 None:intergenic 40.0%
CTACTGAAAATAAGGGAAGG+GGG - chr4.3:28124672-28124691 None:intergenic 40.0%
CTCATACAGGGTCATTGAAA+TGG - chr4.3:28123562-28123581 None:intergenic 40.0%
GAACTGCTGTAACTCATCAA+GGG - chr4.3:28125749-28125768 None:intergenic 40.0%
GATCATGCTTAGCTGAATGT+TGG + chr4.3:28123040-28123059 MS.gene96774:intron 40.0%
GCAATGATCTGACAAATGAG+AGG + chr4.3:28125194-28125213 MS.gene96774:intron 40.0%
GCATCTACAAACTCATTCCA+TGG - chr4.3:28126080-28126099 None:intergenic 40.0%
GGCATTTCAGATTTGTGACA+TGG + chr4.3:28123983-28124002 MS.gene96774:intron 40.0%
GGTTTGGAAATGTGTAGCTT+TGG + chr4.3:28122748-28122767 MS.gene96774:intron 40.0%
TACGTAGCTTAACCCAAAGT+TGG + chr4.3:28124592-28124611 MS.gene96774:intron 40.0%
TACTGAAAATAAGGGAAGGG+GGG - chr4.3:28124671-28124690 None:intergenic 40.0%
TATCTTATTAGCGCTCATGC+AGG + chr4.3:28125973-28125992 MS.gene96774:intron 40.0%
TCAGATATGCATTCCCTTCA+TGG + chr4.3:28125254-28125273 MS.gene96774:intron 40.0%
TTCACCACCATAAATCTCAC+CGG - chr4.3:28125404-28125423 None:intergenic 40.0%
TTGGGAGACCATTATTTGCT+AGG - chr4.3:28124788-28124807 None:intergenic 40.0%
TTGTGACTGTGAGTGAACTA+TGG + chr4.3:28122545-28122564 MS.gene96774:CDS 40.0%
TTTCCTTCTCTCTCCATGAA+GGG - chr4.3:28125270-28125289 None:intergenic 40.0%
TTTCTGCTGGAGAGATTGAT+GGG - chr4.3:28123602-28123621 None:intergenic 40.0%
TTTGAATCAAATGCGTGCAG+CGG + chr4.3:28123886-28123905 MS.gene96774:CDS 40.0%
TTTGTTCAACCATTGTGCTG+TGG + chr4.3:28122506-28122525 MS.gene96774:CDS 40.0%
! ACGTAGATGGATTTGGTAGA+CGG + chr4.3:28123736-28123755 MS.gene96774:intron 40.0%
! CTAACTTGCAGGAAGTAGAA+TGG - chr4.3:28122662-28122681 None:intergenic 40.0%
! GACTCTTATTTGCTTTCTGC+TGG - chr4.3:28123615-28123634 None:intergenic 40.0%
! GCAATTGTACAAGCCTTTTC+CGG - chr4.3:28126031-28126050 None:intergenic 40.0%
! TTTTGCCATGTTCCCATCAT+AGG - chr4.3:28125842-28125861 None:intergenic 40.0%
!! ATAACAGCCAAGTACTGAGT+AGG + chr4.3:28123285-28123304 MS.gene96774:intron 40.0%
!! GAAAGTTGTGGCTCTGAAAA+TGG + chr4.3:28125618-28125637 MS.gene96774:intron 40.0%
!! TAACAGCCAAGTACTGAGTA+GGG + chr4.3:28123286-28123305 MS.gene96774:intron 40.0%
!! TGCTTTTTGGCACCAACTTT+GGG - chr4.3:28124607-28124626 None:intergenic 40.0%
!! TTTCTAAGAGTGTCCTGCAA+CGG + chr4.3:28123860-28123879 MS.gene96774:CDS 40.0%
!!! AGTGGTTTTGTGATGTGGTT+TGG + chr4.3:28122732-28122751 MS.gene96774:intron 40.0%
AAACGGCTCCTTGGAATCAA+AGG - chr4.3:28122466-28122485 None:intergenic 45.0%
AAGCTGCTCCTTTGATTCCA+AGG + chr4.3:28122455-28122474 MS.gene96774:CDS 45.0%
AAGGGGGAGGGAGATATAAA+TGG - chr4.3:28125475-28125494 None:intergenic 45.0%
ACCTAAGGCTTGCATAACCA+AGG - chr4.3:28125350-28125369 None:intergenic 45.0%
ATTCAGATACTTCGCGAGAG+GGG + chr4.3:28124891-28124910 MS.gene96774:CDS 45.0%
ATTCCCTTCATGGAGAGAGA+AGG + chr4.3:28125264-28125283 MS.gene96774:CDS 45.0%
ATTGCAGCCGGTGAGATTTA+TGG + chr4.3:28125394-28125413 MS.gene96774:CDS 45.0%
CAAACATGTCCACAGCACAA+TGG - chr4.3:28122518-28122537 None:intergenic 45.0%
CACACTTTGTTCCCTCACAA+AGG - chr4.3:28126208-28126227 None:intergenic 45.0%
CACCCCTCAAATTGAGAATC+TGG + chr4.3:28124857-28124876 MS.gene96774:CDS 45.0%
CAGCTAAAGGAGTTGTACAG+TGG - chr4.3:28123472-28123491 None:intergenic 45.0%
CATTGTGCTGTGGACATGTT+TGG + chr4.3:28122516-28122535 MS.gene96774:CDS 45.0%
CCATCTTATTGGACCGGAAA+AGG + chr4.3:28126015-28126034 MS.gene96774:CDS 45.0%
CCCCAGATTCTCAATTTGAG+GGG - chr4.3:28124862-28124881 None:intergenic 45.0%
CCCCTCAAATTGAGAATCTG+GGG + chr4.3:28124859-28124878 MS.gene96774:CDS 45.0%
CTCAGATGCAATGCCACAAA+AGG - chr4.3:28124918-28124937 None:intergenic 45.0%
CTGCAAGATTTCAGCTGACA+CGG - chr4.3:28123820-28123839 None:intergenic 45.0%
CTTTCTGCTGGAGAGATTGA+TGG - chr4.3:28123603-28123622 None:intergenic 45.0%
GAGATATGAGATGGACATGC+TGG + chr4.3:28124935-28124954 MS.gene96774:CDS 45.0%
GAGTAAATAGATACCTGCGG+TGG - chr4.3:28125876-28125895 None:intergenic 45.0%
GAGTTGTACAGTGGAGAATC+TGG - chr4.3:28123463-28123482 None:intergenic 45.0%
GATGCACGCCTAGCAAATAA+TGG + chr4.3:28124777-28124796 MS.gene96774:CDS 45.0%
GATTCAGATACTTCGCGAGA+GGG + chr4.3:28124890-28124909 MS.gene96774:CDS 45.0%
GATTTCATGGTTTCGGTAGC+CGG + chr4.3:28125799-28125818 MS.gene96774:CDS 45.0%
GCCCCAGATTCTCAATTTGA+GGG - chr4.3:28124863-28124882 None:intergenic 45.0%
GGAACTGCTGTAACTCATCA+AGG - chr4.3:28125750-28125769 None:intergenic 45.0%
GTACAGTGGAGAATCTGGAT+GGG - chr4.3:28123458-28123477 None:intergenic 45.0%
GTGACATGGTGACAATTGCT+CGG + chr4.3:28123997-28124016 MS.gene96774:CDS 45.0%
TAAGGGAAGGGGGGAAATAT+AGG - chr4.3:28124662-28124681 None:intergenic 45.0%
TCATTTGACCAGCTAACAGG+AGG - chr4.3:28124292-28124311 None:intergenic 45.0%
TCTGAAGAAAGGAGATCCCA+GGG + chr4.3:28125295-28125314 MS.gene96774:CDS 45.0%
TGATTCAGATACTTCGCGAG+AGG + chr4.3:28124889-28124908 MS.gene96774:CDS 45.0%
TGCCCCAGATTCTCAATTTG+AGG - chr4.3:28124864-28124883 None:intergenic 45.0%
TGGGAACATGGCAAAACTTG+TGG + chr4.3:28125846-28125865 MS.gene96774:CDS 45.0%
TGTACAGTGGAGAATCTGGA+TGG - chr4.3:28123459-28123478 None:intergenic 45.0%
TGTAGACAAACCAAAGGAGG+AGG + chr4.3:28126154-28126173 MS.gene96774:CDS 45.0%
TGTCAAAGGACATAGACCCT+GGG - chr4.3:28125314-28125333 None:intergenic 45.0%
TTCATCACAGATGGCTGCTT+TGG + chr4.3:28125777-28125796 MS.gene96774:CDS 45.0%
TTCTGCTGGAGAGATTGATG+GGG - chr4.3:28123601-28123620 None:intergenic 45.0%
TTGCACTAATGAAGAAGCCG+AGG + chr4.3:28124980-28124999 MS.gene96774:CDS 45.0%
! CCTTTTCCGGTCCAATAAGA+TGG - chr4.3:28126018-28126037 None:intergenic 45.0%
! CGTAGATGGATTTGGTAGAC+GGG + chr4.3:28123737-28123756 MS.gene96774:intron 45.0%
! GAACCTCACATTGGTTGTTC+CGG + chr4.3:28124025-28124044 MS.gene96774:CDS 45.0%
! GATGGCTGCTTTGGATTTCA+TGG + chr4.3:28125786-28125805 MS.gene96774:CDS 45.0%
! GCTTTCTCAGGTTGTACTCT+TGG + chr4.3:28124957-28124976 MS.gene96774:CDS 45.0%
! GCTTTTTGCCAAACACAAGG+TGG + chr4.3:28124737-28124756 MS.gene96774:CDS 45.0%
! GTAGATGGATTTGGTAGACG+GGG + chr4.3:28123738-28123757 MS.gene96774:intron 45.0%
! TAGGTGCCGTGTTAATTACC+AGG + chr4.3:28124824-28124843 MS.gene96774:CDS 45.0%
! TCCTACCTATGATGGGAACA+TGG + chr4.3:28125834-28125853 MS.gene96774:CDS 45.0%
! TCGGCTTTTGAACCTCACAT+TGG + chr4.3:28124016-28124035 MS.gene96774:CDS 45.0%
!! GGTAGGAATAAAGGTGTCAC+CGG - chr4.3:28125821-28125840 None:intergenic 45.0%
!! GTGCTTTTTGGCACCAACTT+TGG - chr4.3:28124608-28124627 None:intergenic 45.0%
!! GTTGTGGCTCTGAAAATGGT+TGG + chr4.3:28125622-28125641 MS.gene96774:intron 45.0%
!! TGCAAGCCTTAGGTTTTGAC+CGG + chr4.3:28125356-28125375 MS.gene96774:CDS 45.0%
!! TTCTAAGAGTGTCCTGCAAC+GGG + chr4.3:28123861-28123880 MS.gene96774:CDS 45.0%
!!! ACCGCTTTTTGCCAAACACA+AGG + chr4.3:28124734-28124753 MS.gene96774:CDS 45.0%
!!! GCTTGAGTGGTTTTGTGATG+TGG + chr4.3:28122727-28122746 MS.gene96774:intron 45.0%
AAGAAGCCGAGGATCTCAAG+CGG + chr4.3:28124991-28125010 MS.gene96774:CDS 50.0%
ATAACCAAGGCAGTCTCCTC+CGG - chr4.3:28125337-28125356 None:intergenic 50.0%
ATACCTACGGTCTGCCCAAA+AGG - chr4.3:28124066-28124085 None:intergenic 50.0%
CAAACACGAAAACGGCTCCT+TGG - chr4.3:28122475-28122494 None:intergenic 50.0%
CCTCATGAGTGCCTTTGTGA+GGG + chr4.3:28126194-28126213 MS.gene96774:CDS 50.0%
CGTGTCAGCTGAAATCTTGC+AGG + chr4.3:28123818-28123837 MS.gene96774:intron 50.0%
CTTGCAGGAAGTAGAATGGG+AGG - chr4.3:28122658-28122677 None:intergenic 50.0%
GAAAAGTGGATACTCGACCC+TGG + chr4.3:28124261-28124280 MS.gene96774:CDS 50.0%
GAAGTGCACCACCTTGTGTT+TGG - chr4.3:28124748-28124767 None:intergenic 50.0%
GAGGGGTGAATTTAAGTGCC+TGG - chr4.3:28124845-28124864 None:intergenic 50.0%
GCCATGTTCCCATCATAGGT+AGG - chr4.3:28125838-28125857 None:intergenic 50.0%
GCCTTGGTTATGCAAGCCTT+AGG + chr4.3:28125346-28125365 MS.gene96774:CDS 50.0%
GGGTCTATGTCCTTTGACAC+CGG + chr4.3:28125315-28125334 MS.gene96774:CDS 50.0%
GTCTGAAGAAAGGAGATCCC+AGG + chr4.3:28125294-28125313 MS.gene96774:CDS 50.0%
GTGTCAAAGGACATAGACCC+TGG - chr4.3:28125315-28125334 None:intergenic 50.0%
GTTCCGGAACAACCAATGTG+AGG - chr4.3:28124031-28124050 None:intergenic 50.0%
GTTTGTAGATGCTGTGCGAC+AGG + chr4.3:28126088-28126107 MS.gene96774:CDS 50.0%
TAGCGCTCATGCAGGTATTC+CGG + chr4.3:28125981-28126000 MS.gene96774:intron 50.0%
TCTATGTCCTTTGACACCGG+AGG + chr4.3:28125318-28125337 MS.gene96774:CDS 50.0%
TGTCTCCCGGTCAAAACCTA+AGG - chr4.3:28125365-28125384 None:intergenic 50.0%
TTATTCTGGCAGTGGCTTCG+AGG + chr4.3:28124156-28124175 MS.gene96774:intron 50.0%
TTGATTCAAACCCCCGTTGC+AGG - chr4.3:28123876-28123895 None:intergenic 50.0%
! AATCCTTTTGGGCAGACCGT+AGG + chr4.3:28124060-28124079 MS.gene96774:CDS 50.0%
!! ACAAAGGCACTCATGAGGGT+TGG - chr4.3:28126192-28126211 None:intergenic 50.0%
!! ATGGACATGCTGGCTTTCTC+AGG + chr4.3:28124945-28124964 MS.gene96774:CDS 50.0%
!! CAAGGAGCCGTTTTCGTGTT+TGG + chr4.3:28122473-28122492 MS.gene96774:CDS 50.0%
!! CCAAGTGCTGAAAAATGGGG+AGG - chr4.3:28125595-28125614 None:intergenic 50.0%
!! CCTCACAAAGGCACTCATGA+GGG - chr4.3:28126196-28126215 None:intergenic 50.0%
!! CCTCCCCATTTTTCAGCACT+TGG + chr4.3:28125592-28125611 MS.gene96774:intron 50.0%
!! GCAAGCCTTAGGTTTTGACC+GGG + chr4.3:28125357-28125376 MS.gene96774:CDS 50.0%
!! TCTAAGAGTGTCCTGCAACG+GGG + chr4.3:28123862-28123881 MS.gene96774:CDS 50.0%
ACAACTCAACGGCCTCCTCA+AGG + chr4.3:28122610-28122629 MS.gene96774:CDS 55.0%
CCCTCATGAGTGCCTTTGTG+AGG + chr4.3:28126193-28126212 MS.gene96774:CDS 55.0%
CCTCATCCGCTTGAGATCCT+CGG - chr4.3:28125000-28125019 None:intergenic 55.0%
GCAGCCGGTGAGATTTATGG+TGG + chr4.3:28125397-28125416 MS.gene96774:CDS 55.0%
TGGAGATGCCTCCTGTTAGC+TGG + chr4.3:28124281-28124300 MS.gene96774:CDS 55.0%
!! AGCTAACAGGAGGCATCTCC+AGG - chr4.3:28124282-28124301 None:intergenic 55.0%
!! CCCTCACAAAGGCACTCATG+AGG - chr4.3:28126197-28126216 None:intergenic 55.0%
!! CTAAGAGTGTCCTGCAACGG+GGG + chr4.3:28123863-28123882 MS.gene96774:CDS 55.0%
!! GCTAACAGGAGGCATCTCCA+GGG - chr4.3:28124281-28124300 None:intergenic 55.0%
AACTTGTGGAAGGCCACCGC+AGG + chr4.3:28125860-28125879 MS.gene96774:CDS 60.0%
CCGAGGATCTCAAGCGGATG+AGG + chr4.3:28124997-28125016 MS.gene96774:CDS 60.0%
! GCAGTCTCCTCCGGTGTCAA+AGG - chr4.3:28125328-28125347 None:intergenic 60.0%
! TCGCGAGAGGGGACCTTTTG+TGG + chr4.3:28124902-28124921 MS.gene96774:CDS 60.0%
GACACCGGAGGAGACTGCCT+TGG + chr4.3:28125330-28125349 MS.gene96774:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 28122400 28126268 28122400 ID=MS.gene96774
chr4.3 mRNA 28122400 28126268 28122400 ID=MS.gene96774.t1;Parent=MS.gene96774
chr4.3 exon 28122400 28122676 28122400 ID=MS.gene96774.t1.exon1;Parent=MS.gene96774.t1
chr4.3 CDS 28122400 28122676 28122400 ID=cds.MS.gene96774.t1;Parent=MS.gene96774.t1
chr4.3 exon 28123840 28123907 28123840 ID=MS.gene96774.t1.exon2;Parent=MS.gene96774.t1
chr4.3 CDS 28123840 28123907 28123840 ID=cds.MS.gene96774.t1;Parent=MS.gene96774.t1
chr4.3 exon 28123993 28124081 28123993 ID=MS.gene96774.t1.exon3;Parent=MS.gene96774.t1
chr4.3 CDS 28123993 28124081 28123993 ID=cds.MS.gene96774.t1;Parent=MS.gene96774.t1
chr4.3 exon 28124168 28124330 28124168 ID=MS.gene96774.t1.exon4;Parent=MS.gene96774.t1
chr4.3 CDS 28124168 28124330 28124168 ID=cds.MS.gene96774.t1;Parent=MS.gene96774.t1
chr4.3 exon 28124729 28125018 28124729 ID=MS.gene96774.t1.exon5;Parent=MS.gene96774.t1
chr4.3 CDS 28124729 28125018 28124729 ID=cds.MS.gene96774.t1;Parent=MS.gene96774.t1
chr4.3 exon 28125258 28125462 28125258 ID=MS.gene96774.t1.exon6;Parent=MS.gene96774.t1
chr4.3 CDS 28125258 28125462 28125258 ID=cds.MS.gene96774.t1;Parent=MS.gene96774.t1
chr4.3 exon 28125724 28125881 28125724 ID=MS.gene96774.t1.exon7;Parent=MS.gene96774.t1
chr4.3 CDS 28125724 28125881 28125724 ID=cds.MS.gene96774.t1;Parent=MS.gene96774.t1
chr4.3 exon 28125995 28126268 28125995 ID=MS.gene96774.t1.exon8;Parent=MS.gene96774.t1
chr4.3 CDS 28125995 28126268 28125995 ID=cds.MS.gene96774.t1;Parent=MS.gene96774.t1
Gene Sequence

>MS.gene96774

ATGGAAGTTAGATCATCAGAGGGTAATCAAGTGAGATATTATGATAAGCTTCTTCAAGCTGCTCCTTTGATTCCAAGGAGCCGTTTTCGTGTTTGGTTTATTCGAATTTGTTCAACCATTGTGCTGTGGACATGTTTGGTTCAGCTTGTGACTGTGAGTGAACTATGGCATTCCAATTTCTTTACTGGATTTACTAGTCGTATATATCACACAACTCAACGGCCTCCTCAAGGAGATGTTATATTTGCTCAATCTCCTCCCATTCTACTTCCTGCAAGTTAGTTTTTCTATCTACTTATTTTTCTCAAGATTGCAATGTTTTTGTTTGCTTGAGTGGTTTTGTGATGTGGTTTGGAAATGTGTAGCTTTGGTTGAATCTATGATGCAAACTCTCCACATATGATTTAATTTAGCAATAATAGTAATATGGATTTAATGTATATTCACGGTTAGTGTAAAGATTTTTTACACTGCGAATCAATCATTATCCTCAGATCATTTAAAATGTTTGACTTCAACCATAATTACTTTTAAAGTTAAACATATGATTAGTAGTGTTTTGTTTTGACAATATATCAAAATTCAAATATCATAATATTTATATGATCAACCTAACATTGTGTTTTTATTAGATGGTAACGATCATGCTTAGCTGAATGTTGGGCCATAATGTATGTGTTGATAAACCCTTTGGGAAAAGAATTTATTTTTTTTGGTTTTCTGCTTGTTATATAGTAGGTTTGTAGATTTGTGTGAAGAAACAGGTAGGAGCATGAATGTTAGTGACTTACAATTGTTAATACCAATTAATTGATATTGACCATCATGTTTTTTGGACTTCCTGTTAGAGATGATTTGCTTTGTTGCGTTTTGTGGCCGTAAAAAATAACAGCCAAGTACTGAGTAGGGATAGATACATAGATCAGTTGATGCCATTAGATTCTAACCAAAAATCAATAAATCCATTTAGTTTAGAGTGAAGTCTTTCCCTAATCTTTTTAGTGTACCCCAAAAAAGTGGATCCACTTATTTTAAACTATTGTTGATACTTAATGCCCATCCAGATTCTCCACTGTACAACTCCTTTAGCTGTTCAGTAGAATTTTCCTTTAAATATGATAGGTACTTTTTTATCTCAAATTATGTTTGAAGCATTTGTCCATTTCAATGACCCTGTATGAGTCCTTGTATATAATATCCCCATCAATCTCTCCAGCAGAAAGCAAATAAGAGTCTAACTGAAGATCCCCAATATTACACTTACTTATGTTGAGACATGCATATGCATGCAAGATAGTTTTACATAAGAGTATAAGTAAAATAACAAAATACTTATACGTAGATGGATTTGGTAGACGGGGTATGAGTATCATTAGCTGTTTATATTTTTTCTGATGCACTTTTGTAAATATTAAAGCCGTGTCAGCTGAAATCTTGCAGGAAATTATACTAGTAATGGTTTTCTAAGAGTGTCCTGCAACGGGGGTTTGAATCAAATGCGTGCAGCGGTTTGTACTTCCTATTCTTCCACCACTTCTTTATTCCTATTATATGATAAGCTTCAATGTTTAATTACCAGATTTGGCATTTCAGATTTGTGACATGGTGACAATTGCTCGGCTTTTGAACCTCACATTGGTTGTTCCGGAACTTGATAAGAAATCCTTTTGGGCAGACCGTAGGTATATCATTTGTTATGAAAAAAAAGAGTATTCTCTAATTTGCTTGTGTTGTGTCATGTTAAAGTTGTTTTTTTTTATTCTGGCAGTGGCTTCGAGGATATATTTGATGTAAGACATTTCATTGATTCTTTACGAGATGAAGTTCGAATAGTGAAAAGAGTTCCCAAAAAGTTTACTAGGAAAAGTGGATACTCGACCCTGGAGATGCCTCCTGTTAGCTGGTCAAATGAAAAGTATTACTTGGAGCAGGTCTGCAGATTCTATTTGTTGCACTTAATCTAAAATTATGTATAATACATCAATCAGGTTTTCTCTTATTTGACAATTTAAAGTTTGATACAGTGATTGGTTAATGATTCTTTGATATGGCTTTTAAGACAGCGACATTTAGAGTTATTATCAGATATGGAAACATTTTATAATATCTTGAAATATTAATCATTCATATGAAGAAAAGTACAAGTTTATGATATGGATGGCTTCTAGTATTTTAGCAACTAAAATGGATTGTACGTAGCTTAACCCAAAGTTGGTGCCAAAAAGCACTTTATCTATGTTGAAGTGAAAACTGTAGTTACCTATATTTCCCCCCTTCCCTTATTTTCAGTAGTACATATATTGAGTTTGACTATCGACATTAACTGCAGATTCTACCGCTTTTTGCCAAACACAAGGTGGTGCACTTCAACAAAACAGATGCACGCCTAGCAAATAATGGTCTCCCAATTGATCTACAGAAACTTAGGTGCCGTGTTAATTACCAGGCACTTAAATTCACCCCTCAAATTGAGAATCTGGGGCAAAAATTGATTCAGATACTTCGCGAGAGGGGACCTTTTGTGGCATTGCATCTGAGATATGAGATGGACATGCTGGCTTTCTCAGGTTGTACTCTTGGTTGCACTAATGAAGAAGCCGAGGATCTCAAGCGGATGAGGTATTTTATGTTTCCTGTTTTGCTATGCCTTTTTTATGTATATCTTTTCATGAGCAATAGGTCCTTCTTTTATAATTTGTGTTGCATGATTTGTTGAATTTGGCATATTTTGACAACTACAAATTAAGTACATTCACTTTATAAAAGATTAAGAGTCTTGTGTTCAAAGGAATTTGCAATGATCTGACAAATGAGAGGTCTATCTAAGATAAAATTCTGATTTTGCTTGCATTATTCAGATATGCATTCCCTTCATGGAGAGAGAAGGAAATAGTGTCTGAAGAAAGGAGATCCCAGGGTCTATGTCCTTTGACACCGGAGGAGACTGCCTTGGTTATGCAAGCCTTAGGTTTTGACCGGGAGACACAGATTTACATTGCAGCCGGTGAGATTTATGGTGGTGAACGTAGACTTGCGCAACTGAGAGCTGCATTTCCAAAAATTGTGAGTTCACCATTTATATCTCCCTCCCCCTTTTTGTTTATAGTATTGACATTGACATTTGATATAATAAAGTACCACTGAATGATTCTAGCTTGTGCTTATGCACGATTTTCCATTTATAATTTGTATCCTCCCCATTTTTCAGCACTTGGATTGAAAGTTGTGGCTCTGAAAATGGTTGGCTTAATCTACCTTATGCATTTCTTCATCCTAATCATGGTTACTGCTTGTTTGATAAGATTCCACTTTACTTTACTTTAGGTTAAAAAAGAGGTATTGGTCGCCCTTGATGAGTTACAGCAGTTCCAAAATCATTCATCACAGATGGCTGCTTTGGATTTCATGGTTTCGGTAGCCGGTGACACCTTTATTCCTACCTATGATGGGAACATGGCAAAACTTGTGGAAGGCCACCGCAGGTATCTATTTACTCACGCTAGTTGCAATTTCATCTAATATAGAAGCTATTCTGGTTTTAAAAAACATCCCTAAATTTCCAAAAGAAATATATATCTTATTAGCGCTCATGCAGGTATTCCGGTTTTAAAAAGTCCATCTTATTGGACCGGAAAAGGCTTGTACAATTGCTTGATATGCATCAAAATGGAACCCTTCCATGGAATGAGTTTGTAGATGCTGTGCGACAGGTTCACGAAAAGAGAATGGGTCAGCCGACTTATCGTCGAATCATTGTAGACAAACCAAAGGAGGAGGATTATTTCTACGCCAACCCTCATGAGTGCCTTTGTGAGGGAACAAAGTGTGATGATTTGCTAGGTCCTCATAACTCTACTCAAGTAAGATGA
Protein sequence

>MS.gene96774.t1

MEVRSSEGNQVRYYDKLLQAAPLIPRSRFRVWFIRICSTIVLWTCLVQLVTVSELWHSNFFTGFTSRIYHTTQRPPQGDVIFAQSPPILLPARNYTSNGFLRVSCNGGLNQMRAAICDMVTIARLLNLTLVVPELDKKSFWADRSGFEDIFDVRHFIDSLRDEVRIVKRVPKKFTRKSGYSTLEMPPVSWSNEKYYLEQILPLFAKHKVVHFNKTDARLANNGLPIDLQKLRCRVNYQALKFTPQIENLGQKLIQILRERGPFVALHLRYEMDMLAFSGCTLGCTNEEAEDLKRMRYAFPSWREKEIVSEERRSQGLCPLTPEETALVMQALGFDRETQIYIAAGEIYGGERRLAQLRAAFPKIVKKEVLVALDELQQFQNHSSQMAALDFMVSVAGDTFIPTYDGNMAKLVEGHRRYSGFKKSILLDRKRLVQLLDMHQNGTLPWNEFVDAVRQVHEKRMGQPTYRRIIVDKPKEEDYFYANPHECLCEGTKCDDLLGPHNSTQVR