AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56517

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56517.t1 MTR_4g082310 98.225 507 9 0 1 507 1 507 0.0 1043
MS.gene56517.t1 MTR_5g042200 80.041 486 90 3 28 506 9 494 0.0 823
MS.gene56517.t1 MTR_6g016015 58.799 483 197 2 8 488 25 507 0.0 602
MS.gene56517.t1 MTR_4g110030 61.588 466 173 2 29 488 43 508 0.0 602
MS.gene56517.t1 MTR_2g059350 57.809 493 202 3 8 499 30 517 0.0 596
MS.gene56517.t1 MTR_5g064620 48.675 415 201 4 87 489 97 511 3.70e-140 414
MS.gene56517.t1 MTR_4g125460 48.485 396 197 5 97 490 89 479 6.14e-137 404
MS.gene56517.t1 MTR_4g125460 48.485 396 197 5 97 490 228 618 3.07e-135 405
MS.gene56517.t1 MTR_4g019210 48.371 399 199 5 97 493 232 625 4.50e-134 402
MS.gene56517.t1 MTR_3g099480 47.952 415 201 5 87 489 126 537 2.21e-131 392
MS.gene56517.t1 MTR_5g081690 46.599 397 205 5 96 490 120 511 5.29e-130 388
MS.gene56517.t1 MTR_3g096020 45.735 422 200 8 87 489 175 586 1.76e-123 374
MS.gene56517.t1 MTR_3g115220 42.317 397 214 7 98 492 112 495 1.60e-99 311
MS.gene56517.t1 MTR_4g100810 40.633 411 221 8 93 497 104 497 3.23e-97 304
MS.gene56517.t1 MTR_5g099010 40.393 458 239 11 54 505 90 519 7.29e-97 305
MS.gene56517.t1 MTR_5g012770 41.063 414 210 11 92 499 119 504 7.82e-91 286
MS.gene56517.t1 MTR_5g032100 38.051 431 218 9 97 491 86 503 1.87e-90 285
MS.gene56517.t1 MTR_3g086570 39.487 390 216 7 90 464 144 528 3.05e-89 284
MS.gene56517.t1 MTR_3g100310 36.613 437 228 9 91 491 94 517 1.31e-87 278
MS.gene56517.t1 MTR_1g102030 37.939 427 233 11 83 492 74 485 2.58e-84 270
MS.gene56517.t1 MTR_4g084570 35.096 416 243 10 97 496 150 554 1.49e-81 264
MS.gene56517.t1 MTR_4g132590 37.642 441 220 11 94 489 62 492 1.16e-79 257
MS.gene56517.t1 MTR_4g132590 37.642 441 220 11 94 489 161 591 1.64e-78 257
MS.gene56517.t1 MTR_5g059260 36.650 412 231 10 98 489 158 559 1.90e-78 256
MS.gene56517.t1 MTR_2g016470 35.892 443 231 10 91 490 183 615 5.45e-78 256
MS.gene56517.t1 MTR_1g031590 34.223 412 244 9 97 492 149 549 2.64e-76 250
MS.gene56517.t1 MTR_3g005340 35.507 414 232 11 97 490 147 545 8.71e-74 244
MS.gene56517.t1 MTR_7g064210 71.687 166 37 2 212 368 24 188 1.52e-73 239
MS.gene56517.t1 MTR_3g026650 35.930 398 236 8 100 492 176 559 5.32e-71 236
MS.gene56517.t1 MTR_4g023030 36.409 401 236 8 100 495 236 622 1.39e-70 237
MS.gene56517.t1 MTR_3g086570 39.519 291 160 5 90 366 144 432 1.08e-66 221
MS.gene56517.t1 MTR_1g101990 39.249 293 158 9 206 492 3 281 2.23e-60 201
MS.gene56517.t1 MTR_3g026650 37.618 319 187 5 100 413 176 487 2.58e-59 203
MS.gene56517.t1 MTR_4g010990 33.957 374 221 9 90 457 68 421 1.48e-58 200
MS.gene56517.t1 MTR_4g010990 32.670 352 211 9 112 457 1 332 4.64e-50 175
MS.gene56517.t1 MTR_3g092590 33.010 309 179 8 94 379 82 385 3.54e-42 159
MS.gene56517.t1 MTR_3g092590 33.010 309 179 8 94 379 82 385 1.74e-41 157
MS.gene56517.t1 MTR_7g017330 31.353 303 187 6 85 370 86 384 1.15e-39 152
MS.gene56517.t1 MTR_3g026650 37.662 231 132 5 100 325 176 399 8.02e-37 140
MS.gene56517.t1 MTR_1g007390 30.476 315 185 9 94 379 82 391 1.44e-33 135
MS.gene56517.t1 MTR_1g101985 52.747 91 43 0 83 173 74 164 3.65e-25 102
MS.gene56517.t1 MTR_4g008200 26.608 342 201 13 97 427 35 337 6.15e-24 103
MS.gene56517.t1 MTR_4g008200 26.608 342 201 13 97 427 86 388 1.35e-23 103
MS.gene56517.t1 MTR_2g038320 24.868 378 223 13 66 426 59 392 5.28e-20 92.8
MS.gene56517.t1 MTR_1g052225 37.195 164 53 7 95 258 19 132 2.01e-18 82.0
MS.gene56517.t1 MTR_6g046230 38.318 107 58 2 145 243 34 140 2.19e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56517.t1 AT1G14020 71.683 505 137 2 1 505 1 499 0.0 758
MS.gene56517.t1 AT2G03280 69.528 466 130 4 24 488 26 480 0.0 686
MS.gene56517.t1 AT2G03280 65.720 493 130 5 24 488 26 507 0.0 671
MS.gene56517.t1 AT5G15740 58.030 467 194 2 23 488 33 498 0.0 580
MS.gene56517.t1 AT3G02250 58.281 477 191 4 15 488 27 498 0.0 572
MS.gene56517.t1 AT2G37980 46.453 437 227 5 73 507 202 633 1.09e-137 412
MS.gene56517.t1 AT3G54100 46.065 432 216 7 73 497 198 619 1.11e-133 401
MS.gene56517.t1 AT5G35570 47.407 405 206 5 96 498 250 649 2.12e-132 399
MS.gene56517.t1 AT1G22460 45.602 432 215 6 76 490 130 558 5.01e-130 390
MS.gene56517.t1 AT5G64600 46.445 422 197 8 87 489 103 514 3.05e-128 384
MS.gene56517.t1 AT5G01100 47.132 401 204 5 97 493 216 612 3.65e-128 387
MS.gene56517.t1 AT1G76270 44.836 397 203 7 100 494 109 491 1.83e-104 324
MS.gene56517.t1 AT1G20550 42.647 408 218 7 100 505 112 505 6.80e-103 320
MS.gene56517.t1 AT4G38390 43.500 400 212 7 97 494 123 510 1.51e-102 318
MS.gene56517.t1 AT4G16650 43.276 409 208 10 89 490 113 504 2.51e-94 297
MS.gene56517.t1 AT4G16650 43.276 409 208 10 89 490 113 504 2.51e-94 297
MS.gene56517.t1 AT1G04910 36.853 464 259 10 50 506 57 493 2.01e-91 288
MS.gene56517.t1 AT4G24530 37.674 430 219 9 97 490 101 517 1.09e-89 284
MS.gene56517.t1 AT3G26370 38.974 390 218 7 90 464 152 536 2.52e-89 284
MS.gene56517.t1 AT5G65470 35.581 430 226 9 99 490 87 503 1.68e-86 275
MS.gene56517.t1 AT1G11990 39.173 411 222 10 98 491 191 590 1.92e-82 267
MS.gene56517.t1 AT1G62330 34.862 436 232 8 98 489 222 649 3.31e-82 268
MS.gene56517.t1 AT1G38065 36.427 431 238 11 79 489 24 438 6.55e-82 262
MS.gene56517.t1 AT1G38065 37.046 413 230 10 97 489 26 428 7.71e-82 262
MS.gene56517.t1 AT1G38131 37.046 413 230 10 97 489 155 557 2.07e-80 262
MS.gene56517.t1 AT1G11990 36.161 448 221 10 98 491 176 612 1.98e-77 254
MS.gene56517.t1 AT1G29200 37.931 435 219 11 97 490 70 494 2.01e-76 249
MS.gene56517.t1 AT1G29200 37.931 435 219 11 97 490 70 494 2.01e-76 249
MS.gene56517.t1 AT5G63390 38.000 400 228 8 100 493 167 552 3.90e-76 250
MS.gene56517.t1 AT1G29200 37.931 435 219 11 97 490 186 610 3.98e-75 248
MS.gene56517.t1 AT1G35510 35.012 417 240 10 92 490 154 557 7.52e-75 247
MS.gene56517.t1 AT2G01480 36.314 369 213 7 97 449 154 516 2.22e-74 245
MS.gene56517.t1 AT1G14970 34.140 413 245 10 97 493 9 410 2.75e-74 241
MS.gene56517.t1 AT1G29200 37.762 429 216 11 103 490 2 420 3.51e-74 241
MS.gene56517.t1 AT2G01480 36.695 357 207 6 97 437 154 507 5.86e-74 243
MS.gene56517.t1 AT1G14970 34.140 413 245 10 97 493 35 436 7.03e-74 241
MS.gene56517.t1 AT1G14970 34.140 413 245 10 97 493 155 556 6.03e-73 241
MS.gene56517.t1 AT2G44500 36.962 395 231 8 100 489 180 561 1.32e-72 241
MS.gene56517.t1 AT3G07900 33.824 408 253 6 84 489 180 572 1.76e-68 230
MS.gene56517.t1 AT1G52630 32.708 373 224 10 90 458 70 419 4.66e-59 201
MS.gene56517.t1 AT3G03810 35.855 304 178 7 91 379 83 384 7.73e-50 181
MS.gene56517.t1 AT3G03810 35.855 304 178 7 91 379 83 384 7.73e-50 181
MS.gene56517.t1 AT1G52630 31.054 351 218 9 112 458 1 331 7.85e-50 174
MS.gene56517.t1 AT3G26370 39.171 217 119 5 90 296 152 365 8.14e-46 165
MS.gene56517.t1 AT3G30300 33.333 306 180 7 91 379 97 395 2.58e-43 163
MS.gene56517.t1 AT3G30300 33.333 306 180 7 91 379 97 395 2.58e-43 163
MS.gene56517.t1 AT2G44500 38.793 232 131 5 100 326 180 405 1.07e-38 146
MS.gene56517.t1 AT1G51630 24.845 322 192 9 96 406 90 372 1.09e-18 89.0

Find 129 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATCTGCGATGAATGATTT+TGG 0.192845 4.4:-30295467 None:intergenic
ATTGCAGCCGGTGAGATTTA+TGG 0.227102 4.4:+30295093 MS.gene56517:CDS
TGGCATTCCAATTTCTTTAC+TGG 0.232585 4.4:+30292262 MS.gene56517:CDS
TCAGATATGCATTCCCTTCA+TGG 0.261168 4.4:+30294953 MS.gene56517:intron
GATGGCTGCGTTGGATTTCA+TGG 0.273261 4.4:+30295485 MS.gene56517:CDS
AGTAAATCCAGTAAAGAAAT+TGG 0.278555 4.4:-30292269 None:intergenic
GCTTTCTCAGGTTGCACTCT+TGG 0.280074 4.4:+30294656 MS.gene56517:CDS
CACCCCTCAAATTGAGAATC+TGG 0.293410 4.4:+30294556 MS.gene56517:CDS
TTCTAAGAGTGTCCTGCAAT+GGG 0.310538 4.4:+30293560 MS.gene56517:CDS
TTGCTTGATATGCATCAAAA+TGG 0.313563 4.4:+30295746 MS.gene56517:CDS
CTAAGTTTCTGTAGATCAAT+TGG 0.331955 4.4:-30294503 None:intergenic
AAGGATTTCTTATCAAGTTC+CGG 0.337356 4.4:-30293743 None:intergenic
AACTTGATAAGAAATCCTTT+TGG 0.353716 4.4:+30293747 MS.gene56517:CDS
TAGAAATAATCCTCCTCCTT+TGG 0.360561 4.4:-30295863 None:intergenic
TAGCTCTCATGCAGGTATTC+CGG 0.361952 4.4:+30295680 MS.gene56517:intron
TGCGTTGGATTTCATGGTTT+CGG 0.364096 4.4:+30295491 MS.gene56517:CDS
CATTGTGCTGTGGACATGTT+TGG 0.367522 4.4:+30292213 MS.gene56517:CDS
TTATTCTGGCAGTGGCTTTG+AGG 0.370478 4.4:+30293854 MS.gene56517:intron
ACCTTTATTCCTACCTATGA+TGG 0.388352 4.4:+30295525 MS.gene56517:CDS
AGCTAACAGGAGGCATCTCC+AGG 0.388940 4.4:-30293977 None:intergenic
ATGGACATGCTGGCTTTCTC+AGG 0.395187 4.4:+30294644 MS.gene56517:CDS
TGCCCCAGATTCTCAATTTG+AGG 0.396144 4.4:-30294560 None:intergenic
GAACCTCACATTGGTTGTTC+CGG 0.404001 4.4:+30293724 MS.gene56517:CDS
CCATCTTATTGGACCGGAAA+AGG 0.405140 4.4:+30295714 MS.gene56517:CDS
GCCTTGGTTATGCAAGCCTT+AGG 0.432969 4.4:+30295045 MS.gene56517:CDS
CCCATCATAGGTAGGAATAA+AGG 0.436770 4.4:-30295526 None:intergenic
GCAGGAAATTATACTAGTAA+TGG 0.439060 4.4:+30293535 MS.gene56517:intron
TTTCTAAGAGTGTCCTGCAA+TGG 0.444915 4.4:+30293559 MS.gene56517:CDS
ATACCTACGGTCTGCCCAAA+AGG 0.445579 4.4:-30293762 None:intergenic
AGGAAATAGTGTCTGAAGAA+AGG 0.445827 4.4:+30294983 MS.gene56517:CDS
GATTTCATGGTTTCGGTAGC+CGG 0.450519 4.4:+30295498 MS.gene56517:CDS
GGAACTGCTGTAACTCATCA+AGG 0.452325 4.4:-30295446 None:intergenic
AAAAGTCCATCTTATTGGAC+CGG 0.458534 4.4:+30295708 MS.gene56517:CDS
GAAGTGCACCACCTTGTGTT+TGG 0.463947 4.4:-30294444 None:intergenic
AAGCTGCTCCTTTGATTCCA+AGG 0.468221 4.4:+30292152 MS.gene56517:CDS
CTTACTTGAGTAGAGTTATG+AGG 0.468781 4.4:-30295941 None:intergenic
TTGGGAGACCATTATTTGCT+AGG 0.469220 4.4:-30294484 None:intergenic
GCCCCAGATTCTCAATTTGA+GGG 0.469715 4.4:-30294559 None:intergenic
GAATAAACCAAACACGAAAA+CGG 0.470528 4.4:-30292177 None:intergenic
CTAACTTGCAGGAAGTAGAA+TGG 0.473374 4.4:-30292356 None:intergenic
TAAGTTTCTGTAGATCAATT+GGG 0.475386 4.4:-30294502 None:intergenic
TGGAGATGCCTCCTGTTAGC+TGG 0.475776 4.4:+30293979 MS.gene56517:CDS
TTGTGACTGTGAGTGAACTA+TGG 0.476628 4.4:+30292242 MS.gene56517:CDS
TAACTTGCAGGAAGTAGAAT+GGG 0.479064 4.4:-30292355 None:intergenic
CAAACACGAAAACGGCTCCT+TGG 0.481546 4.4:-30292169 None:intergenic
ACCCCTCAAATTGAGAATCT+GGG 0.484445 4.4:+30294557 MS.gene56517:CDS
GAGGGGTGAATTTAAGTGCC+TGG 0.487600 4.4:-30294541 None:intergenic
GTCAAATGAAAAGTATTACT+TGG 0.489028 4.4:+30294001 MS.gene56517:CDS
CTCAGATGCAATGCCACAAA+AGG 0.490594 4.4:-30294614 None:intergenic
TTCATCGCAGATGGCTGCGT+TGG 0.491891 4.4:+30295476 MS.gene56517:CDS
ATTTCCTTCTCTCTCCATGA+AGG 0.500444 4.4:-30294967 None:intergenic
GATGCACGCCTAGCAAATAA+TGG 0.504541 4.4:+30294476 MS.gene56517:CDS
ATAACCAAGGCAGTCTCCTC+CGG 0.505709 4.4:-30295033 None:intergenic
CAATTGATCTACAGAAACTT+AGG 0.505738 4.4:+30294504 MS.gene56517:CDS
GTCTGAAGAAAGGAGATCAC+AGG 0.505868 4.4:+30294993 MS.gene56517:CDS
ACAGGTTCACGAAAAGAGAA+TGG 0.506682 4.4:+30295805 MS.gene56517:CDS
CCGAGGATCTCAAGCGGATG+AGG 0.509548 4.4:+30294696 MS.gene56517:CDS
GCAGTCTCCTCCGGTGTCAA+AGG 0.517244 4.4:-30295024 None:intergenic
GACACCGGAGGAGACTGCCT+TGG 0.525025 4.4:+30295029 MS.gene56517:CDS
GCATCTACAAACTCATTCCA+TGG 0.525256 4.4:-30295776 None:intergenic
CTAAGAGTGTCCTGCAATGG+GGG 0.532495 4.4:+30293562 MS.gene56517:CDS
CCTCATCCGCTTGAGATCCT+CGG 0.534275 4.4:-30294696 None:intergenic
GTTTGTAGATGCTGTGCGAC+AGG 0.534296 4.4:+30295787 MS.gene56517:CDS
CCTTTATTCCTACCTATGAT+GGG 0.535492 4.4:+30295526 MS.gene56517:CDS
TGAAAAGTATTACTTGGAGC+AGG 0.539964 4.4:+30294007 MS.gene56517:CDS
TGTCTCCCGGTCAAAACCTA+AGG 0.543702 4.4:-30295061 None:intergenic
CTACAAACTCATTCCATGGA+AGG 0.551044 4.4:-30295772 None:intergenic
AAACGGCTCCTTGGAATCAA+AGG 0.552528 4.4:-30292160 None:intergenic
CCCCAGATTCTCAATTTGAG+GGG 0.555415 4.4:-30294558 None:intergenic
CCAAAATCATTCATCGCAGA+TGG 0.556343 4.4:+30295467 MS.gene56517:CDS
GAGATATGAGATGGACATGC+TGG 0.557518 4.4:+30294634 MS.gene56517:CDS
CAGGTTCACGAAAAGAGAAT+GGG 0.560835 4.4:+30295806 MS.gene56517:CDS
ATCAAAATGGAACCCTTCCA+TGG 0.561331 4.4:+30295759 MS.gene56517:CDS
CAATGTAAATCTGTGTCTCC+CGG 0.564211 4.4:-30295074 None:intergenic
TTTCCTTCTCTCTCCATGAA+GGG 0.565721 4.4:-30294966 None:intergenic
CCCTCATGAGTGCCTTTGTG+AGG 0.571802 4.4:+30295892 MS.gene56517:CDS
TCTAAGAGTGTCCTGCAATG+GGG 0.572836 4.4:+30293561 MS.gene56517:CDS
ACACAGATTTACATTGCAGC+CGG 0.575721 4.4:+30295081 MS.gene56517:CDS
ACAATGATTCGACGATAAGT+CGG 0.577569 4.4:-30295833 None:intergenic
GTGACATGGTGACAATTGCT+CGG 0.579350 4.4:+30293696 MS.gene56517:CDS
TGGGAACATGGCAAAACTTG+TGG 0.580054 4.4:+30295545 MS.gene56517:CDS
TAGGTGCCGTGTTAATTACC+AGG 0.582283 4.4:+30294523 MS.gene56517:CDS
GATTCAGATACTTCGCGAGA+GGG 0.583952 4.4:+30294589 MS.gene56517:CDS
TGTAGACAAACCAAAGGAGG+AGG 0.587728 4.4:+30295853 MS.gene56517:CDS
GGGTCTATGTCCTTTGACAC+CGG 0.590187 4.4:+30295014 MS.gene56517:CDS
TACAAACTCATTCCATGGAA+GGG 0.592326 4.4:-30295771 None:intergenic
GGTAGGAATAAAGGTGTCAC+CGG 0.594798 4.4:-30295517 None:intergenic
ACAAAGGCACTCATGAGGGT+TGG 0.596296 4.4:-30295888 None:intergenic
CCCTCACAAAGGCACTCATG+AGG 0.597403 4.4:-30295893 None:intergenic
CACACTTTGTTCCCTCACAA+AGG 0.600880 4.4:-30295904 None:intergenic
AACTTGTGGAAGGCCACCGC+AGG 0.602466 4.4:+30295559 MS.gene56517:CDS
CCTCACAAAGGCACTCATGA+GGG 0.602588 4.4:-30295892 None:intergenic
GCCATGTTCCCATCATAGGT+AGG 0.602990 4.4:-30295534 None:intergenic
GGCGTTTCAGATTTGTGACA+TGG 0.604590 4.4:+30293682 MS.gene56517:intron
CCCCTCAAATTGAGAATCTG+GGG 0.605300 4.4:+30294558 MS.gene56517:CDS
ACAACTCAACGGCCTCCTCA+AGG 0.607711 4.4:+30292307 MS.gene56517:CDS
AACATGGCAAAACTTGTGGA+AGG 0.609196 4.4:+30295549 MS.gene56517:CDS
CCTCATGAGTGCCTTTGTGA+GGG 0.609759 4.4:+30295893 MS.gene56517:CDS
TCCTACCTATGATGGGAACA+TGG 0.610654 4.4:+30295533 MS.gene56517:CDS
TTTGAATCAAATGCGTGCAG+CGG 0.612731 4.4:+30293585 MS.gene56517:CDS
ACAAAGTGTGATGATTTGCT+AGG 0.614058 4.4:+30295917 MS.gene56517:CDS
AATGGAAGTTAGATCATCAG+AGG 0.614061 4.4:+30292096 None:intergenic
CATTGTAGACAAACCAAAGG+AGG 0.615228 4.4:+30295850 MS.gene56517:CDS
TTCACCACCATAAATCTCAC+CGG 0.615230 4.4:-30295100 None:intergenic
TCATTTGACCAGCTAACAGG+AGG 0.621119 4.4:-30293987 None:intergenic
ACCTAAGGCTTGCATAACCA+AGG 0.627242 4.4:-30295046 None:intergenic
GTATATATCACACAACTCAA+CGG 0.629814 4.4:+30292296 MS.gene56517:CDS
AATCATTGTAGACAAACCAA+AGG 0.637419 4.4:+30295847 MS.gene56517:CDS
ATTCAGATACTTCGCGAGAG+GGG 0.637675 4.4:+30294590 MS.gene56517:CDS
GAACTGCTGTAACTCATCAA+GGG 0.638982 4.4:-30295445 None:intergenic
TTTGTTCAACCATTGTGCTG+TGG 0.645471 4.4:+30292203 MS.gene56517:CDS
TGATTCAGATACTTCGCGAG+AGG 0.649037 4.4:+30294588 MS.gene56517:CDS
ATTCCCTTCATGGAGAGAGA+AGG 0.650807 4.4:+30294963 MS.gene56517:CDS
CTTGCAGGAAGTAGAATGGG+AGG 0.651294 4.4:-30292352 None:intergenic
GAGTAAATATATACCTGCGG+TGG 0.652300 4.4:-30295572 None:intergenic
GAAAAGTGGATACTCGACCC+TGG 0.652908 4.4:+30293959 MS.gene56517:CDS
AATATAACATCTCCTTGAGG+AGG 0.657753 4.4:-30292319 None:intergenic
TAAGTGCCTGGTAATTAACA+CGG 0.657828 4.4:-30294529 None:intergenic
ATGGAAGTTAGATCATCAGA+GGG 0.666260 4.4:+30292097 MS.gene56517:CDS
TTGCACTAATGAAGAAGCCG+AGG 0.669813 4.4:+30294679 MS.gene56517:CDS
CAAACATGTCCACAGCACAA+TGG 0.677892 4.4:-30292212 None:intergenic
AAGAAGCCGAGGATCTCAAG+CGG 0.678663 4.4:+30294690 MS.gene56517:CDS
TCTGAAGAAAGGAGATCACA+GGG 0.680384 4.4:+30294994 MS.gene56517:CDS
GCTAACAGGAGGCATCTCCA+GGG 0.682429 4.4:-30293976 None:intergenic
ATTGCATCTGAGATATGAGA+TGG 0.683390 4.4:+30294625 MS.gene56517:CDS
TCTATGTCCTTTGACACCGG+AGG 0.698582 4.4:+30295017 MS.gene56517:CDS
GCAAATATAACATCTCCTTG+AGG 0.698776 4.4:-30292322 None:intergenic
GTTCCGGAACAACCAATGTG+AGG 0.701296 4.4:-30293727 None:intergenic
GCAGCCGGTGAGATTTATGG+TGG 0.721413 4.4:+30295096 MS.gene56517:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTTTTTGGAAATTTA+GGG - chr4.4:30295651-30295670 None:intergenic 10.0%
!!! TATATTTTTTTTGGAAATTT+AGG - chr4.4:30295652-30295671 None:intergenic 10.0%
!!! TTTAACTTTAAAAGTAATTA+TGG - chr4.4:30292618-30292637 None:intergenic 10.0%
!! ATTTAGCAATAATAGTAATA+TGG + chr4.4:30292504-30292523 MS.gene56517:intron 15.0%
!! TGAAAAGATATACATAAAAA+AGG - chr4.4:30294748-30294767 None:intergenic 15.0%
!!! AATTTTCCTTTAAATATGAT+AGG + chr4.4:30293199-30293218 MS.gene56517:intron 15.0%
!!! TAAAGTTGTTTTTTTTATTC+TGG + chr4.4:30293840-30293859 MS.gene56517:intron 15.0%
!!! TAAATGGATTTATTGATTTT+TGG - chr4.4:30293048-30293067 None:intergenic 15.0%
!! AACATAAGTAAGTGTAATAT+TGG - chr4.4:30293350-30293369 None:intergenic 20.0%
!! ACATAAGTAAGTGTAATATT+GGG - chr4.4:30293349-30293368 None:intergenic 20.0%
!! ATATAATAGGAATAAAGAAG+TGG - chr4.4:30293632-30293651 None:intergenic 20.0%
!! ATATGTACTACTGAAAATAA+GGG - chr4.4:30294378-30294397 None:intergenic 20.0%
!! ATGGATTTAATGTATATTCA+CGG + chr4.4:30292523-30292542 MS.gene56517:intron 20.0%
!! ATTGAAGCTTATCATATAAT+AGG - chr4.4:30293645-30293664 None:intergenic 20.0%
!! GTCAATACTATAAACAAAAA+GGG - chr4.4:30295192-30295211 None:intergenic 20.0%
!! TATATGTACTACTGAAAATA+AGG - chr4.4:30294379-30294398 None:intergenic 20.0%
!! TCAATACTATAAACAAAAAG+GGG - chr4.4:30295191-30295210 None:intergenic 20.0%
!! TCTAATAAAAACACAATGTT+AGG - chr4.4:30292710-30292729 None:intergenic 20.0%
!! TGTCAATACTATAAACAAAA+AGG - chr4.4:30295193-30295212 None:intergenic 20.0%
!! TTATGTATAATACATCAATC+AGG + chr4.4:30294064-30294083 MS.gene56517:intron 20.0%
!! TTGCTAAATTAAATCATATG+TGG - chr4.4:30292493-30292512 None:intergenic 20.0%
!!! AAAAGTACAAGTTTATGATA+TGG + chr4.4:30294232-30294251 MS.gene56517:intron 20.0%
!!! AAAATAAATTCTTTTCCCAA+AGG - chr4.4:30292787-30292806 None:intergenic 20.0%
!!! AAAGTACCTATCATATTTAA+AGG - chr4.4:30293208-30293227 None:intergenic 20.0%
!!! AAATAAATTCTTTTCCCAAA+GGG - chr4.4:30292786-30292805 None:intergenic 20.0%
!!! AACATTGTGTTTTTATTAGA+TGG + chr4.4:30292710-30292729 MS.gene56517:intron 20.0%
!!! CAACAATAGTTTAAAATAAG+TGG - chr4.4:30293124-30293143 None:intergenic 20.0%
!!! TAGTATTTTAGCAACTAAAA+TGG + chr4.4:30294263-30294282 MS.gene56517:intron 20.0%
!!! TTTACTTTAGGTTAAAAAAG+AGG + chr4.4:30295413-30295432 MS.gene56517:intron 20.0%
!!! TTTTAAAAAGTCCATCTTAT+TGG + chr4.4:30295703-30295722 MS.gene56517:CDS 20.0%
! ACAAAATACTTATACGTAGA+TGG + chr4.4:30293422-30293441 MS.gene56517:intron 25.0%
! AGTAAATCCAGTAAAGAAAT+TGG - chr4.4:30292272-30292291 None:intergenic 25.0%
! ATCTAATATAGAAGCTATTC+TGG + chr4.4:30295612-30295631 MS.gene56517:intron 25.0%
! ATTCCACTTTACTTTACTTT+AGG + chr4.4:30295401-30295420 MS.gene56517:intron 25.0%
! CAAAATACTTATACGTAGAT+GGG + chr4.4:30293423-30293442 MS.gene56517:intron 25.0%
! CAATACTATAAACAAAAAGG+GGG - chr4.4:30295190-30295209 None:intergenic 25.0%
! CAATGTTTAATTACCAGATT+TGG + chr4.4:30293661-30293680 MS.gene56517:intron 25.0%
! CATAACAAATGATATACCTA+CGG - chr4.4:30293778-30293797 None:intergenic 25.0%
! CATAAGTAAGTGTAATATTG+GGG - chr4.4:30293348-30293367 None:intergenic 25.0%
! GATGGTCAATATCAATTAAT+TGG - chr4.4:30292904-30292923 None:intergenic 25.0%
! GCAACACAAATTATAAAAGA+AGG - chr4.4:30294783-30294802 None:intergenic 25.0%
! GTTTATCAACACATACATTA+TGG - chr4.4:30292764-30292783 None:intergenic 25.0%
! TAACCTAAAGTAAAGTAAAG+TGG - chr4.4:30295407-30295426 None:intergenic 25.0%
! TAAGTTTCTGTAGATCAATT+GGG - chr4.4:30294505-30294524 None:intergenic 25.0%
! TGGTTAATGATTCTTTGATA+TGG + chr4.4:30294126-30294145 MS.gene56517:intron 25.0%
! TTAAAGTTTGATACAGTGAT+TGG + chr4.4:30294106-30294125 MS.gene56517:intron 25.0%
!! AACTTGATAAGAAATCCTTT+TGG + chr4.4:30293747-30293766 MS.gene56517:CDS 25.0%
!! ACTTGATAAGAAATCCTTTT+GGG + chr4.4:30293748-30293767 MS.gene56517:CDS 25.0%
!! CAAACATTTTAAATGATCTG+AGG - chr4.4:30292588-30292607 None:intergenic 25.0%
!! TATATCTTTTCATGAGCAAT+AGG + chr4.4:30294756-30294775 MS.gene56517:intron 25.0%
!! TTAGGTTAAAAAAGAGGTAT+TGG + chr4.4:30295419-30295438 MS.gene56517:intron 25.0%
!! TTTAGAGTTATTAGCAGATA+TGG + chr4.4:30294166-30294185 MS.gene56517:intron 25.0%
!!! AACAATGATAAAGTGCTTTT+TGG - chr4.4:30294318-30294337 None:intergenic 25.0%
!!! ATATTGACCATCATGTTTTT+TGG + chr4.4:30292912-30292931 MS.gene56517:intron 25.0%
!!! GATTTTTGGTTAGAATCTAA+TGG - chr4.4:30293034-30293053 None:intergenic 25.0%
!!! GTCAAATGAAAAGTATTACT+TGG + chr4.4:30294001-30294020 MS.gene56517:CDS 25.0%
!!! TAAGATGGACTTTTTAAAAC+CGG - chr4.4:30295702-30295721 None:intergenic 25.0%
!!! TACTTGGCTGTTATTTTTTA+CGG - chr4.4:30292978-30292997 None:intergenic 25.0%
!!! TTTTCTGCTTGTTATATAGT+AGG + chr4.4:30292815-30292834 MS.gene56517:intron 25.0%
AAAAAGTTTACTGGGAAAAG+TGG + chr4.4:30293945-30293964 MS.gene56517:CDS 30.0%
AAATTCTACTGAACAGCTAA+AGG - chr4.4:30293184-30293203 None:intergenic 30.0%
AAGGATTTCTTATCAAGTTC+CGG - chr4.4:30293746-30293765 None:intergenic 30.0%
AATCATTGTAGACAAACCAA+AGG + chr4.4:30295847-30295866 MS.gene56517:CDS 30.0%
AGACTTCACTCTAAACTAAA+TGG - chr4.4:30293064-30293083 None:intergenic 30.0%
CAATTGATCTACAGAAACTT+AGG + chr4.4:30294504-30294523 MS.gene56517:CDS 30.0%
CTAAGTTTCTGTAGATCAAT+TGG - chr4.4:30294506-30294525 None:intergenic 30.0%
CTTGAGTAAATATATACCTG+CGG - chr4.4:30295578-30295597 None:intergenic 30.0%
GAATAAACCAAACACGAAAA+CGG - chr4.4:30292180-30292199 None:intergenic 30.0%
GCAGGAAATTATACTAGTAA+TGG + chr4.4:30293535-30293554 MS.gene56517:intron 30.0%
GGGAGGATACAAATTATAAA+TGG - chr4.4:30295277-30295296 None:intergenic 30.0%
GTACAAGTTTATGATATGGA+TGG + chr4.4:30294236-30294255 MS.gene56517:intron 30.0%
GTATATATCACACAACTCAA+CGG + chr4.4:30292296-30292315 MS.gene56517:CDS 30.0%
TAATAGGAATAAAGAAGTGG+TGG - chr4.4:30293629-30293648 None:intergenic 30.0%
TACTACTGAAAATAAGGGAA+GGG - chr4.4:30294373-30294392 None:intergenic 30.0%
TATATACAAGGACTCATACA+GGG - chr4.4:30293273-30293292 None:intergenic 30.0%
TGATGGGGATATTATATACA+AGG - chr4.4:30293285-30293304 None:intergenic 30.0%
TTAGGATGAAGAAATGCATA+AGG - chr4.4:30295356-30295375 None:intergenic 30.0%
TTATATACAAGGACTCATAC+AGG - chr4.4:30293274-30293293 None:intergenic 30.0%
TTGCTTGATATGCATCAAAA+TGG + chr4.4:30295746-30295765 MS.gene56517:CDS 30.0%
! AATGGTGAACTCACAATTTT+TGG - chr4.4:30295156-30295175 None:intergenic 30.0%
! ACTTTTCCCAGTAAACTTTT+TGG - chr4.4:30293946-30293965 None:intergenic 30.0%
! AGATAGAAAAACTAACTTGC+AGG - chr4.4:30292370-30292389 None:intergenic 30.0%
! ATTAAGAGTCTTGTGTTCAA+AGG + chr4.4:30294865-30294884 MS.gene56517:intron 30.0%
! CTTTTCCCAGTAAACTTTTT+GGG - chr4.4:30293945-30293964 None:intergenic 30.0%
! TAAAAAAGGCATAGCAAAAC+AGG - chr4.4:30294734-30294753 None:intergenic 30.0%
! TATGTGTTGATAAACCCTTT+GGG + chr4.4:30292769-30292788 MS.gene56517:intron 30.0%
!! AATGTTTTTGTTTGCTTGAG+TGG + chr4.4:30292411-30292430 MS.gene56517:intron 30.0%
!! GTTGCATGATTTGTTGAATT+TGG + chr4.4:30294798-30294817 MS.gene56517:intron 30.0%
!!! AATAAGTGGATCCACTTTTT+TGG - chr4.4:30293110-30293129 None:intergenic 30.0%
!!! ATAAGTGGATCCACTTTTTT+GGG - chr4.4:30293109-30293128 None:intergenic 30.0%
!!! TGTTTTTTTTATTCTGGCAG+TGG + chr4.4:30293846-30293865 MS.gene56517:intron 30.0%
AAAAGTCCATCTTATTGGAC+CGG + chr4.4:30295708-30295727 MS.gene56517:CDS 35.0%
AACAAGCAGTAACCATGATT+AGG - chr4.4:30295374-30295393 None:intergenic 35.0%
AATATAACATCTCCTTGAGG+AGG - chr4.4:30292322-30292341 None:intergenic 35.0%
ACAATGATTCGACGATAAGT+CGG - chr4.4:30295836-30295855 None:intergenic 35.0%
ACCTTTATTCCTACCTATGA+TGG + chr4.4:30295525-30295544 MS.gene56517:CDS 35.0%
ACTACTGAAAATAAGGGAAG+GGG - chr4.4:30294372-30294391 None:intergenic 35.0%
ACTATAAACAAAAAGGGGGA+GGG - chr4.4:30295186-30295205 None:intergenic 35.0%
AGAGTTCCCAAAAAGTTTAC+TGG + chr4.4:30293936-30293955 MS.gene56517:CDS 35.0%
AGGAAATAGTGTCTGAAGAA+AGG + chr4.4:30294983-30295002 MS.gene56517:CDS 35.0%
AGGAAGTCCAAAAAACATGA+TGG - chr4.4:30292922-30292941 None:intergenic 35.0%
ATCATGCTTAGCTGAATGTT+GGG + chr4.4:30292738-30292757 MS.gene56517:intron 35.0%
ATGGAAGTTAGATCATCAGA+GGG + chr4.4:30292097-30292116 MS.gene56517:CDS 35.0%
ATTTGTGTGAAGAAACAGGT+AGG + chr4.4:30292845-30292864 MS.gene56517:intron 35.0%
CAAAGCAAATCATCTCTAAC+AGG - chr4.4:30292942-30292961 None:intergenic 35.0%
CCTTTATTCCTACCTATGAT+GGG + chr4.4:30295526-30295545 MS.gene56517:CDS 35.0%
CTTACTTGAGTAGAGTTATG+AGG - chr4.4:30295944-30295963 None:intergenic 35.0%
GAGTTCCCAAAAAGTTTACT+GGG + chr4.4:30293937-30293956 MS.gene56517:CDS 35.0%
GCAAATATAACATCTCCTTG+AGG - chr4.4:30292325-30292344 None:intergenic 35.0%
GCATTTCTTCATCCTAATCA+TGG + chr4.4:30295359-30295378 MS.gene56517:intron 35.0%
GGGGTACACTAAAAAGATTA+GGG - chr4.4:30293089-30293108 None:intergenic 35.0%
GTAATGATCTGACAAATGAG+AGG + chr4.4:30294893-30294912 MS.gene56517:intron 35.0%
GTACTACTGAAAATAAGGGA+AGG - chr4.4:30294374-30294393 None:intergenic 35.0%
GTAGATTTGTGTGAAGAAAC+AGG + chr4.4:30292841-30292860 MS.gene56517:intron 35.0%
TAAGTGCCTGGTAATTAACA+CGG - chr4.4:30294532-30294551 None:intergenic 35.0%
TACAAACTCATTCCATGGAA+GGG - chr4.4:30295774-30295793 None:intergenic 35.0%
TACTATAAACAAAAAGGGGG+AGG - chr4.4:30295187-30295206 None:intergenic 35.0%
TAGAAATAATCCTCCTCCTT+TGG - chr4.4:30295866-30295885 None:intergenic 35.0%
TGGCATTCCAATTTCTTTAC+TGG + chr4.4:30292262-30292281 MS.gene56517:CDS 35.0%
TGGGGTACACTAAAAAGATT+AGG - chr4.4:30293090-30293109 None:intergenic 35.0%
TTTAGTGTACCCCAAAAAAG+TGG + chr4.4:30293096-30293115 MS.gene56517:intron 35.0%
! ATTGCATCTGAGATATGAGA+TGG + chr4.4:30294625-30294644 MS.gene56517:CDS 35.0%
! GTATGTGTTGATAAACCCTT+TGG + chr4.4:30292768-30292787 MS.gene56517:intron 35.0%
! TAAAGAAGTGGTGGAAGAAT+AGG - chr4.4:30293620-30293639 None:intergenic 35.0%
! TAACTTGCAGGAAGTAGAAT+GGG - chr4.4:30292358-30292377 None:intergenic 35.0%
! TACTTATACGTAGATGGGTT+TGG + chr4.4:30293428-30293447 MS.gene56517:intron 35.0%
! TATCTTATTAGCTCTCATGC+AGG + chr4.4:30295672-30295691 MS.gene56517:intron 35.0%
! TTTTCATTTGACCAGCTAAC+AGG - chr4.4:30293993-30294012 None:intergenic 35.0%
!! AATCCAAGTGCTGAAAAATG+GGG - chr4.4:30295297-30295316 None:intergenic 35.0%
!! ACAAAGTGTGATGATTTGCT+AGG + chr4.4:30295917-30295936 MS.gene56517:CDS 35.0%
!! ATTTGCTTTGTTGCGTTTTG+TGG + chr4.4:30292952-30292971 MS.gene56517:intron 35.0%
!! CAATCCAAGTGCTGAAAAAT+GGG - chr4.4:30295298-30295317 None:intergenic 35.0%
!! TCAATCCAAGTGCTGAAAAA+TGG - chr4.4:30295299-30295318 None:intergenic 35.0%
!! TGAAAAGTATTACTTGGAGC+AGG + chr4.4:30294007-30294026 MS.gene56517:CDS 35.0%
!!! TAAGTGGATCCACTTTTTTG+GGG - chr4.4:30293108-30293127 None:intergenic 35.0%
AACATGGCAAAACTTGTGGA+AGG + chr4.4:30295549-30295568 MS.gene56517:CDS 40.0%
ACACAGATTTACATTGCAGC+CGG + chr4.4:30295081-30295100 MS.gene56517:CDS 40.0%
ACAGGTTCACGAAAAGAGAA+TGG + chr4.4:30295805-30295824 MS.gene56517:CDS 40.0%
ACCCCTCAAATTGAGAATCT+GGG + chr4.4:30294557-30294576 MS.gene56517:CDS 40.0%
ACCTTGTGTTTGGCAAAAAG+CGG - chr4.4:30294437-30294456 None:intergenic 40.0%
AGCACTTGGATTGAAAGTTG+TGG + chr4.4:30295305-30295324 MS.gene56517:intron 40.0%
ATCAAAATGGAACCCTTCCA+TGG + chr4.4:30295759-30295778 MS.gene56517:CDS 40.0%
ATCTATCCCTACTCAGTACT+TGG - chr4.4:30292994-30293013 None:intergenic 40.0%
ATTTCCTTCTCTCTCCATGA+AGG - chr4.4:30294970-30294989 None:intergenic 40.0%
CAATGTAAATCTGTGTCTCC+CGG - chr4.4:30295077-30295096 None:intergenic 40.0%
CACAAGCTAGAATCATTCAG+TGG - chr4.4:30295239-30295258 None:intergenic 40.0%
CAGGTTCACGAAAAGAGAAT+GGG + chr4.4:30295806-30295825 MS.gene56517:CDS 40.0%
CATTGTAGACAAACCAAAGG+AGG + chr4.4:30295850-30295869 MS.gene56517:CDS 40.0%
CCAAAATCATTCATCGCAGA+TGG + chr4.4:30295467-30295486 MS.gene56517:CDS 40.0%
CCCATCATAGGTAGGAATAA+AGG - chr4.4:30295529-30295548 None:intergenic 40.0%
CTACAAACTCATTCCATGGA+AGG - chr4.4:30295775-30295794 None:intergenic 40.0%
CTACTGAAAATAAGGGAAGG+GGG - chr4.4:30294371-30294390 None:intergenic 40.0%
CTCATACAGGGTCATTGAAA+TGG - chr4.4:30293261-30293280 None:intergenic 40.0%
GAACTGCTGTAACTCATCAA+GGG - chr4.4:30295448-30295467 None:intergenic 40.0%
GAGTAAATATATACCTGCGG+TGG - chr4.4:30295575-30295594 None:intergenic 40.0%
GATCATGCTTAGCTGAATGT+TGG + chr4.4:30292737-30292756 MS.gene56517:intron 40.0%
GCATCTACAAACTCATTCCA+TGG - chr4.4:30295779-30295798 None:intergenic 40.0%
GGTTTGGAAATGTGTAGCTT+TGG + chr4.4:30292445-30292464 MS.gene56517:intron 40.0%
TACGTAGCTTAACCCAAAGT+TGG + chr4.4:30294290-30294309 MS.gene56517:intron 40.0%
TACTGAAAATAAGGGAAGGG+GGG - chr4.4:30294370-30294389 None:intergenic 40.0%
TCAGATATGCATTCCCTTCA+TGG + chr4.4:30294953-30294972 MS.gene56517:intron 40.0%
TCTGAAGAAAGGAGATCACA+GGG + chr4.4:30294994-30295013 MS.gene56517:CDS 40.0%
TGCGTTGGATTTCATGGTTT+CGG + chr4.4:30295491-30295510 MS.gene56517:CDS 40.0%
TTCACCACCATAAATCTCAC+CGG - chr4.4:30295103-30295122 None:intergenic 40.0%
TTGGGAGACCATTATTTGCT+AGG - chr4.4:30294487-30294506 None:intergenic 40.0%
TTGTGACTGTGAGTGAACTA+TGG + chr4.4:30292242-30292261 MS.gene56517:CDS 40.0%
TTTCCTTCTCTCTCCATGAA+GGG - chr4.4:30294969-30294988 None:intergenic 40.0%
TTTCTGCTGGAGAGATTGAT+GGG - chr4.4:30293301-30293320 None:intergenic 40.0%
TTTGAATCAAATGCGTGCAG+CGG + chr4.4:30293585-30293604 MS.gene56517:CDS 40.0%
TTTGTTCAACCATTGTGCTG+TGG + chr4.4:30292203-30292222 MS.gene56517:CDS 40.0%
! CAAATCTGAAACGCCAAATC+TGG - chr4.4:30293677-30293696 None:intergenic 40.0%
! CCATCTGCGATGAATGATTT+TGG - chr4.4:30295470-30295489 None:intergenic 40.0%
! CTAACTTGCAGGAAGTAGAA+TGG - chr4.4:30292359-30292378 None:intergenic 40.0%
! GACTCTTATTTGCTTTCTGC+TGG - chr4.4:30293314-30293333 None:intergenic 40.0%
! GCAATTGTACAAGCCTTTTC+CGG - chr4.4:30295730-30295749 None:intergenic 40.0%
! TTTTGCCATGTTCCCATCAT+AGG - chr4.4:30295541-30295560 None:intergenic 40.0%
!! ATAACAGCCAAGTACTGAGT+AGG + chr4.4:30292984-30293003 MS.gene56517:intron 40.0%
!! GAAAGTTGTGGCTCTGAAAA+TGG + chr4.4:30295317-30295336 MS.gene56517:intron 40.0%
!! TAACAGCCAAGTACTGAGTA+GGG + chr4.4:30292985-30293004 MS.gene56517:intron 40.0%
!! TGCTTTTTGGCACCAACTTT+GGG - chr4.4:30294305-30294324 None:intergenic 40.0%
!! TTCTAAGAGTGTCCTGCAAT+GGG + chr4.4:30293560-30293579 MS.gene56517:CDS 40.0%
!! TTTCTAAGAGTGTCCTGCAA+TGG + chr4.4:30293559-30293578 MS.gene56517:CDS 40.0%
!!! AGTGGTTTTGTGATGTGGTT+TGG + chr4.4:30292429-30292448 MS.gene56517:intron 40.0%
AAACGGCTCCTTGGAATCAA+AGG - chr4.4:30292163-30292182 None:intergenic 45.0%
AAGCTGCTCCTTTGATTCCA+AGG + chr4.4:30292152-30292171 MS.gene56517:CDS 45.0%
AAGGGGGAGGGAGATATAAA+TGG - chr4.4:30295174-30295193 None:intergenic 45.0%
ACCTAAGGCTTGCATAACCA+AGG - chr4.4:30295049-30295068 None:intergenic 45.0%
ATTCAGATACTTCGCGAGAG+GGG + chr4.4:30294590-30294609 MS.gene56517:CDS 45.0%
ATTCCCTTCATGGAGAGAGA+AGG + chr4.4:30294963-30294982 MS.gene56517:CDS 45.0%
ATTGCAGCCGGTGAGATTTA+TGG + chr4.4:30295093-30295112 MS.gene56517:CDS 45.0%
CAAACATGTCCACAGCACAA+TGG - chr4.4:30292215-30292234 None:intergenic 45.0%
CACACTTTGTTCCCTCACAA+AGG - chr4.4:30295907-30295926 None:intergenic 45.0%
CACCCCTCAAATTGAGAATC+TGG + chr4.4:30294556-30294575 MS.gene56517:CDS 45.0%
CAGCTAAAGGAGTTGTACAG+TGG - chr4.4:30293171-30293190 None:intergenic 45.0%
CATTGTGCTGTGGACATGTT+TGG + chr4.4:30292213-30292232 MS.gene56517:CDS 45.0%
CCATCTTATTGGACCGGAAA+AGG + chr4.4:30295714-30295733 MS.gene56517:CDS 45.0%
CCCCAGATTCTCAATTTGAG+GGG - chr4.4:30294561-30294580 None:intergenic 45.0%
CCCCTCAAATTGAGAATCTG+GGG + chr4.4:30294558-30294577 MS.gene56517:CDS 45.0%
CTCAGATGCAATGCCACAAA+AGG - chr4.4:30294617-30294636 None:intergenic 45.0%
CTGCAAGATTTCAGCTGACA+CGG - chr4.4:30293519-30293538 None:intergenic 45.0%
CTTTCTGCTGGAGAGATTGA+TGG - chr4.4:30293302-30293321 None:intergenic 45.0%
GAGATATGAGATGGACATGC+TGG + chr4.4:30294634-30294653 MS.gene56517:CDS 45.0%
GAGTTGTACAGTGGAGAATC+TGG - chr4.4:30293162-30293181 None:intergenic 45.0%
GATGCACGCCTAGCAAATAA+TGG + chr4.4:30294476-30294495 MS.gene56517:CDS 45.0%
GATTCAGATACTTCGCGAGA+GGG + chr4.4:30294589-30294608 MS.gene56517:CDS 45.0%
GATTTCATGGTTTCGGTAGC+CGG + chr4.4:30295498-30295517 MS.gene56517:CDS 45.0%
GCCCCAGATTCTCAATTTGA+GGG - chr4.4:30294562-30294581 None:intergenic 45.0%
GGAACTGCTGTAACTCATCA+AGG - chr4.4:30295449-30295468 None:intergenic 45.0%
GGCGTTTCAGATTTGTGACA+TGG + chr4.4:30293682-30293701 MS.gene56517:intron 45.0%
GTACAGTGGAGAATCTGGAT+GGG - chr4.4:30293157-30293176 None:intergenic 45.0%
GTCTGAAGAAAGGAGATCAC+AGG + chr4.4:30294993-30295012 MS.gene56517:CDS 45.0%
GTGACATGGTGACAATTGCT+CGG + chr4.4:30293696-30293715 MS.gene56517:CDS 45.0%
TAAGGGAAGGGGGGAAATAT+AGG - chr4.4:30294361-30294380 None:intergenic 45.0%
TAGCTCTCATGCAGGTATTC+CGG + chr4.4:30295680-30295699 MS.gene56517:intron 45.0%
TCATTTGACCAGCTAACAGG+AGG - chr4.4:30293990-30294009 None:intergenic 45.0%
TGATTCAGATACTTCGCGAG+AGG + chr4.4:30294588-30294607 MS.gene56517:CDS 45.0%
TGCCCCAGATTCTCAATTTG+AGG - chr4.4:30294563-30294582 None:intergenic 45.0%
TGGGAACATGGCAAAACTTG+TGG + chr4.4:30295545-30295564 MS.gene56517:CDS 45.0%
TGTACAGTGGAGAATCTGGA+TGG - chr4.4:30293158-30293177 None:intergenic 45.0%
TGTAGACAAACCAAAGGAGG+AGG + chr4.4:30295853-30295872 MS.gene56517:CDS 45.0%
TTCTGCTGGAGAGATTGATG+GGG - chr4.4:30293300-30293319 None:intergenic 45.0%
TTGATTCAAACCCCCATTGC+AGG - chr4.4:30293575-30293594 None:intergenic 45.0%
TTGCACTAATGAAGAAGCCG+AGG + chr4.4:30294679-30294698 MS.gene56517:CDS 45.0%
! ACGTAGATGGGTTTGGTAGA+CGG + chr4.4:30293435-30293454 MS.gene56517:intron 45.0%
! CCTTTTCCGGTCCAATAAGA+TGG - chr4.4:30295717-30295736 None:intergenic 45.0%
! GAACCTCACATTGGTTGTTC+CGG + chr4.4:30293724-30293743 MS.gene56517:CDS 45.0%
! GCTTTTTGCCAAACACAAGG+TGG + chr4.4:30294436-30294455 MS.gene56517:CDS 45.0%
! TAGGTGCCGTGTTAATTACC+AGG + chr4.4:30294523-30294542 MS.gene56517:CDS 45.0%
! TCCTACCTATGATGGGAACA+TGG + chr4.4:30295533-30295552 MS.gene56517:CDS 45.0%
! TCGGCTTTTGAACCTCACAT+TGG + chr4.4:30293715-30293734 MS.gene56517:CDS 45.0%
! TTATTCTGGCAGTGGCTTTG+AGG + chr4.4:30293854-30293873 MS.gene56517:intron 45.0%
!! GGTAGGAATAAAGGTGTCAC+CGG - chr4.4:30295520-30295539 None:intergenic 45.0%
!! GTGCTTTTTGGCACCAACTT+TGG - chr4.4:30294306-30294325 None:intergenic 45.0%
!! GTTGTGGCTCTGAAAATGGT+TGG + chr4.4:30295321-30295340 MS.gene56517:intron 45.0%
!! TCTAAGAGTGTCCTGCAATG+GGG + chr4.4:30293561-30293580 MS.gene56517:CDS 45.0%
!! TGCAAGCCTTAGGTTTTGAC+CGG + chr4.4:30295055-30295074 MS.gene56517:CDS 45.0%
!!! ACCGCTTTTTGCCAAACACA+AGG + chr4.4:30294433-30294452 MS.gene56517:CDS 45.0%
!!! GCTTGAGTGGTTTTGTGATG+TGG + chr4.4:30292424-30292443 MS.gene56517:intron 45.0%
!!! AAAAGAATTTATTTTTTTTT+TGG + chr4.4:30292792-30292811 MS.gene56517:intron 5.0%
!!! AATAAGATATATATTTTTTT+TGG - chr4.4:30295661-30295680 None:intergenic 5.0%
AAGAAGCCGAGGATCTCAAG+CGG + chr4.4:30294690-30294709 MS.gene56517:CDS 50.0%
ATAACCAAGGCAGTCTCCTC+CGG - chr4.4:30295036-30295055 None:intergenic 50.0%
ATACCTACGGTCTGCCCAAA+AGG - chr4.4:30293765-30293784 None:intergenic 50.0%
CAAACACGAAAACGGCTCCT+TGG - chr4.4:30292172-30292191 None:intergenic 50.0%
CCTCATGAGTGCCTTTGTGA+GGG + chr4.4:30295893-30295912 MS.gene56517:CDS 50.0%
CGTGTCAGCTGAAATCTTGC+AGG + chr4.4:30293517-30293536 MS.gene56517:intron 50.0%
CTTGCAGGAAGTAGAATGGG+AGG - chr4.4:30292355-30292374 None:intergenic 50.0%
GAAAAGTGGATACTCGACCC+TGG + chr4.4:30293959-30293978 MS.gene56517:CDS 50.0%
GAAGTGCACCACCTTGTGTT+TGG - chr4.4:30294447-30294466 None:intergenic 50.0%
GAGGGGTGAATTTAAGTGCC+TGG - chr4.4:30294544-30294563 None:intergenic 50.0%
GATGGCTGCGTTGGATTTCA+TGG + chr4.4:30295485-30295504 MS.gene56517:CDS 50.0%
GCCATGTTCCCATCATAGGT+AGG - chr4.4:30295537-30295556 None:intergenic 50.0%
GCCTTGGTTATGCAAGCCTT+AGG + chr4.4:30295045-30295064 MS.gene56517:CDS 50.0%
GGGTCTATGTCCTTTGACAC+CGG + chr4.4:30295014-30295033 MS.gene56517:CDS 50.0%
GTTCCGGAACAACCAATGTG+AGG - chr4.4:30293730-30293749 None:intergenic 50.0%
GTTTGTAGATGCTGTGCGAC+AGG + chr4.4:30295787-30295806 MS.gene56517:CDS 50.0%
TCTATGTCCTTTGACACCGG+AGG + chr4.4:30295017-30295036 MS.gene56517:CDS 50.0%
TGTCTCCCGGTCAAAACCTA+AGG - chr4.4:30295064-30295083 None:intergenic 50.0%
! AATCCTTTTGGGCAGACCGT+AGG + chr4.4:30293759-30293778 MS.gene56517:CDS 50.0%
! CGTAGATGGGTTTGGTAGAC+GGG + chr4.4:30293436-30293455 MS.gene56517:intron 50.0%
! GCTTTCTCAGGTTGCACTCT+TGG + chr4.4:30294656-30294675 MS.gene56517:CDS 50.0%
! GTAGATGGGTTTGGTAGACG+GGG + chr4.4:30293437-30293456 MS.gene56517:intron 50.0%
!! ACAAAGGCACTCATGAGGGT+TGG - chr4.4:30295891-30295910 None:intergenic 50.0%
!! ATGGACATGCTGGCTTTCTC+AGG + chr4.4:30294644-30294663 MS.gene56517:CDS 50.0%
!! CAAGGAGCCGTTTTCGTGTT+TGG + chr4.4:30292170-30292189 MS.gene56517:CDS 50.0%
!! CCAAGTGCTGAAAAATGGGG+AGG - chr4.4:30295294-30295313 None:intergenic 50.0%
!! CCTCACAAAGGCACTCATGA+GGG - chr4.4:30295895-30295914 None:intergenic 50.0%
!! CCTCCCCATTTTTCAGCACT+TGG + chr4.4:30295291-30295310 MS.gene56517:intron 50.0%
!! CTAAGAGTGTCCTGCAATGG+GGG + chr4.4:30293562-30293581 MS.gene56517:CDS 50.0%
!! GCAAGCCTTAGGTTTTGACC+GGG + chr4.4:30295056-30295075 MS.gene56517:CDS 50.0%
ACAACTCAACGGCCTCCTCA+AGG + chr4.4:30292307-30292326 MS.gene56517:CDS 55.0%
CCCTCATGAGTGCCTTTGTG+AGG + chr4.4:30295892-30295911 MS.gene56517:CDS 55.0%
CCTCATCCGCTTGAGATCCT+CGG - chr4.4:30294699-30294718 None:intergenic 55.0%
GCAGCCGGTGAGATTTATGG+TGG + chr4.4:30295096-30295115 MS.gene56517:CDS 55.0%
TGGAGATGCCTCCTGTTAGC+TGG + chr4.4:30293979-30293998 MS.gene56517:CDS 55.0%
TTCATCGCAGATGGCTGCGT+TGG + chr4.4:30295476-30295495 MS.gene56517:CDS 55.0%
!! AGCTAACAGGAGGCATCTCC+AGG - chr4.4:30293980-30293999 None:intergenic 55.0%
!! CCCTCACAAAGGCACTCATG+AGG - chr4.4:30295896-30295915 None:intergenic 55.0%
!! GCTAACAGGAGGCATCTCCA+GGG - chr4.4:30293979-30293998 None:intergenic 55.0%
AACTTGTGGAAGGCCACCGC+AGG + chr4.4:30295559-30295578 MS.gene56517:CDS 60.0%
CCGAGGATCTCAAGCGGATG+AGG + chr4.4:30294696-30294715 MS.gene56517:CDS 60.0%
! GCAGTCTCCTCCGGTGTCAA+AGG - chr4.4:30295027-30295046 None:intergenic 60.0%
! TCGCGAGAGGGGACCTTTTG+TGG + chr4.4:30294601-30294620 MS.gene56517:CDS 60.0%
GACACCGGAGGAGACTGCCT+TGG + chr4.4:30295029-30295048 MS.gene56517:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 30292097 30295967 30292097 ID=MS.gene56517
chr4.4 mRNA 30292097 30295967 30292097 ID=MS.gene56517.t1;Parent=MS.gene56517
chr4.4 exon 30292097 30292373 30292097 ID=MS.gene56517.t1.exon1;Parent=MS.gene56517.t1
chr4.4 CDS 30292097 30292373 30292097 ID=cds.MS.gene56517.t1;Parent=MS.gene56517.t1
chr4.4 exon 30293539 30293606 30293539 ID=MS.gene56517.t1.exon2;Parent=MS.gene56517.t1
chr4.4 CDS 30293539 30293606 30293539 ID=cds.MS.gene56517.t1;Parent=MS.gene56517.t1
chr4.4 exon 30293692 30293780 30293692 ID=MS.gene56517.t1.exon3;Parent=MS.gene56517.t1
chr4.4 CDS 30293692 30293780 30293692 ID=cds.MS.gene56517.t1;Parent=MS.gene56517.t1
chr4.4 exon 30293866 30294028 30293866 ID=MS.gene56517.t1.exon4;Parent=MS.gene56517.t1
chr4.4 CDS 30293866 30294028 30293866 ID=cds.MS.gene56517.t1;Parent=MS.gene56517.t1
chr4.4 exon 30294428 30294717 30294428 ID=MS.gene56517.t1.exon5;Parent=MS.gene56517.t1
chr4.4 CDS 30294428 30294717 30294428 ID=cds.MS.gene56517.t1;Parent=MS.gene56517.t1
chr4.4 exon 30294957 30295161 30294957 ID=MS.gene56517.t1.exon6;Parent=MS.gene56517.t1
chr4.4 CDS 30294957 30295161 30294957 ID=cds.MS.gene56517.t1;Parent=MS.gene56517.t1
chr4.4 exon 30295423 30295580 30295423 ID=MS.gene56517.t1.exon7;Parent=MS.gene56517.t1
chr4.4 CDS 30295423 30295580 30295423 ID=cds.MS.gene56517.t1;Parent=MS.gene56517.t1
chr4.4 exon 30295694 30295967 30295694 ID=MS.gene56517.t1.exon8;Parent=MS.gene56517.t1
chr4.4 CDS 30295694 30295967 30295694 ID=cds.MS.gene56517.t1;Parent=MS.gene56517.t1
Gene Sequence

>MS.gene56517

ATGGAAGTTAGATCATCAGAGGGTAATCAAGTGAGATATTATGATAAGCTTCTTCAAGCTGCTCCTTTGATTCCAAGGAGCCGTTTTCGTGTTTGGTTTATTCGAATTTGTTCAACCATTGTGCTGTGGACATGTTTGGTTCAGCTTGTGACTGTGAGTGAACTATGGCATTCCAATTTCTTTACTGGATTTACTAGTCGTATATATCACACAACTCAACGGCCTCCTCAAGGAGATGTTATATTTGCTCAATCTCCTCCCATTCTACTTCCTGCAAGTTAGTTTTTCTATCTACTTATTTTTCTCAAGATTGCAATGTTTTTGTTTGCTTGAGTGGTTTTGTGATGTGGTTTGGAAATGTGTAGCTTTGGTTGAATCTATGATGCAAACTCTCCACATATGATTTAATTTAGCAATAATAGTAATATGGATTTAATGTATATTCACGGTTAGTGTAAAGATTTTTTACACTGCGAATCAATCATTATCCTCAGATCATTTAAAATGTTTGACTTCAACCATAATTACTTTTAAAGTTAAACATATGATTAGTAGTGTTTTGTTTTGACAATATATCAAAATTCAAATATCATAATATTTATATGATCAACCTAACATTGTGTTTTTATTAGATGGTAATGATCATGCTTAGCTGAATGTTGGGCCATAATGTATGTGTTGATAAACCCTTTGGGAAAAGAATTTATTTTTTTTTTGGTTTTCTGCTTGTTATATAGTAGGTTTGTAGATTTGTGTGAAGAAACAGGTAGGAGCATGAATGTTAGTGACTTACAATTGTTAATACCAATTAATTGATATTGACCATCATGTTTTTTGGACTTCCTGTTAGAGATGATTTGCTTTGTTGCGTTTTGTGGCCGTAAAAAATAACAGCCAAGTACTGAGTAGGGATAGATACATAGATCAGTTGATGCCATTAGATTCTAACCAAAAATCAATAAATCCATTTAGTTTAGAGTGAAGTCTTTCCCTAATCTTTTTAGTGTACCCCAAAAAAGTGGATCCACTTATTTTAAACTATTGTTGATATTTAATGCCCATCCAGATTCTCCACTGTACAACTCCTTTAGCTGTTCAGTAGAATTTTCCTTTAAATATGATAGGTACTTTTTTATCTCAAATTATGTTTGAAGCATTTGTCCATTTCAATGACCCTGTATGAGTCCTTGTATATAATATCCCCATCAATCTCTCCAGCAGAAAGCAAATAAGAGTCTAACTGAAGATCCCCAATATTACACTTACTTATGTTGAGACATGCATATGCATGCAAGATAGTTTTACATAAGAATATAAGTAAAATAACAAAATACTTATACGTAGATGGGTTTGGTAGACGGGGTATGAGTATCATTAGCTGTTTATATTTTTTCTGATGCACTTTTGTAAATATTAAAGCCGTGTCAGCTGAAATCTTGCAGGAAATTATACTAGTAATGGTTTTCTAAGAGTGTCCTGCAATGGGGGTTTGAATCAAATGCGTGCAGCGGTTTGTACTTCCTATTCTTCCACCACTTCTTTATTCCTATTATATGATAAGCTTCAATGTTTAATTACCAGATTTGGCGTTTCAGATTTGTGACATGGTGACAATTGCTCGGCTTTTGAACCTCACATTGGTTGTTCCGGAACTTGATAAGAAATCCTTTTGGGCAGACCGTAGGTATATCATTTGTTATGAAAAAAAAAAGTATTCTCTAATTTGCTTGTGTTGTGTCATGTTAAAGTTGTTTTTTTTATTCTGGCAGTGGCTTTGAGGATATATTTGATGTAAGACATTTCATTGATTCTTTACGAGATGAAGTTCGAATAGTGAAAAGAGTTCCCAAAAAGTTTACTGGGAAAAGTGGATACTCGACCCTGGAGATGCCTCCTGTTAGCTGGTCAAATGAAAAGTATTACTTGGAGCAGGTCTGCAGATTCTATTTGTTGCACTTAATCTAAAATTATGTATAATACATCAATCAGGTTTTCTCTTATTTGACAATTTAAAGTTTGATACAGTGATTGGTTAATGATTCTTTGATATGGCTTTTAAGACAGCGACATTTAGAGTTATTAGCAGATATGGAAACATTTTATAATATCTTGAAATACTAATCATTCATATGAAGAAAAGTACAAGTTTATGATATGGATGGCTTCTAGTATTTTAGCAACTAAAATGGATTGTACGTAGCTTAACCCAAAGTTGGTGCCAAAAAGCACTTTATCATTGTTGAAGTGAAAAACTGTAGTTACCTATATTTCCCCCCTTCCCTTATTTTCAGTAGTACATATATTGAGTTTGACTATCGACTCTAACTGCAGATTCTACCGCTTTTTGCCAAACACAAGGTGGTGCACTTCAACAAAACAGATGCACGCCTAGCAAATAATGGTCTCCCAATTGATCTACAGAAACTTAGGTGCCGTGTTAATTACCAGGCACTTAAATTCACCCCTCAAATTGAGAATCTGGGGCAAAAATTGATTCAGATACTTCGCGAGAGGGGACCTTTTGTGGCATTGCATCTGAGATATGAGATGGACATGCTGGCTTTCTCAGGTTGCACTCTTGGTTGCACTAATGAAGAAGCCGAGGATCTCAAGCGGATGAGGTATTTTATGTTTCCTGTTTTGCTATGCCTTTTTTATGTATATCTTTTCATGAGCAATAGGTCCTTCTTTTATAATTTGTGTTGCATGATTTGTTGAATTTGGCATATTTTGACAACTACAAATTAAGTACATTCACTTCATAAAAGATTAAGAGTCTTGTGTTCAAAGGAATTTGTAATGATCTGACAAATGAGAGGTCTATCTAAGATAAAATTCTGATTTTGCTTGCATTATTCAGATATGCATTCCCTTCATGGAGAGAGAAGGAAATAGTGTCTGAAGAAAGGAGATCACAGGGTCTATGTCCTTTGACACCGGAGGAGACTGCCTTGGTTATGCAAGCCTTAGGTTTTGACCGGGAGACACAGATTTACATTGCAGCCGGTGAGATTTATGGTGGTGAACGTAGACTTGCGCAACTGAGAGCTGCATTTCCAAAAATTGTGAGTTCACCATTTATATCTCCCTCCCCCTTTTTGTTTATAGTATTGACATTGACATTTGATGTAATAAAGTACCACTGAATGATTCTAGCTTGTGCTTATGCACGATTTTCCATTTATAATTTGTATCCTCCCCATTTTTCAGCACTTGGATTGAAAGTTGTGGCTCTGAAAATGGTTGGCTTAATCTACCTTATGCATTTCTTCATCCTAATCATGGTTACTGCTTGTTTGATAAGATTCCACTTTACTTTACTTTAGGTTAAAAAAGAGGTATTGGTCGCCCTTGATGAGTTACAGCAGTTCCAAAATCATTCATCGCAGATGGCTGCGTTGGATTTCATGGTTTCGGTAGCCGGTGACACCTTTATTCCTACCTATGATGGGAACATGGCAAAACTTGTGGAAGGCCACCGCAGGTATATATTTACTCAAGCTAGTTGCAATATTATCTAATATAGAAGCTATTCTGGTTTTAAAAAACATCCCTAAATTTCCAAAAAAAATATATATCTTATTAGCTCTCATGCAGGTATTCCGGTTTTAAAAAGTCCATCTTATTGGACCGGAAAAGGCTTGTACAATTGCTTGATATGCATCAAAATGGAACCCTTCCATGGAATGAGTTTGTAGATGCTGTGCGACAGGTTCACGAAAAGAGAATGGGTCAGCCGACTTATCGTCGAATCATTGTAGACAAACCAAAGGAGGAGGATTATTTCTACGCCAACCCTCATGAGTGCCTTTGTGAGGGAACAAAGTGTGATGATTTGCTAGGTCCTCATAACTCTACTCAAGTAAGATGA
Protein sequence

>MS.gene56517.t1

MEVRSSEGNQVRYYDKLLQAAPLIPRSRFRVWFIRICSTIVLWTCLVQLVTVSELWHSNFFTGFTSRIYHTTQRPPQGDVIFAQSPPILLPARNYTSNGFLRVSCNGGLNQMRAAICDMVTIARLLNLTLVVPELDKKSFWADRSGFEDIFDVRHFIDSLRDEVRIVKRVPKKFTGKSGYSTLEMPPVSWSNEKYYLEQILPLFAKHKVVHFNKTDARLANNGLPIDLQKLRCRVNYQALKFTPQIENLGQKLIQILRERGPFVALHLRYEMDMLAFSGCTLGCTNEEAEDLKRMRYAFPSWREKEIVSEERRSQGLCPLTPEETALVMQALGFDRETQIYIAAGEIYGGERRLAQLRAAFPKIVKKEVLVALDELQQFQNHSSQMAALDFMVSVAGDTFIPTYDGNMAKLVEGHRRYSGFKKSILLDRKRLVQLLDMHQNGTLPWNEFVDAVRQVHEKRMGQPTYRRIIVDKPKEEDYFYANPHECLCEGTKCDDLLGPHNSTQVR