AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53071

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53071.t1 MTR_5g042200 96.559 494 13 2 1 490 1 494 0.0 995
MS.gene53071.t1 MTR_4g082310 79.668 482 95 2 9 490 28 506 0.0 821
MS.gene53071.t1 MTR_4g110030 61.111 468 175 3 10 472 43 508 0.0 603
MS.gene53071.t1 MTR_6g016015 59.871 466 182 3 9 472 45 507 0.0 602
MS.gene53071.t1 MTR_2g059350 60.805 472 173 4 3 472 46 507 0.0 600
MS.gene53071.t1 MTR_5g064620 49.392 411 196 4 75 473 101 511 2.50e-139 411
MS.gene53071.t1 MTR_4g125460 47.739 398 201 5 81 476 89 481 2.01e-135 400
MS.gene53071.t1 MTR_4g125460 47.739 398 201 5 81 476 228 620 7.39e-134 400
MS.gene53071.t1 MTR_4g019210 47.912 407 200 6 76 475 222 623 1.45e-133 400
MS.gene53071.t1 MTR_3g099480 47.887 426 203 6 68 477 119 541 5.12e-132 393
MS.gene53071.t1 MTR_5g081690 46.482 398 206 5 80 475 120 512 2.58e-129 385
MS.gene53071.t1 MTR_3g096020 47.433 409 190 7 84 477 192 590 2.58e-127 383
MS.gene53071.t1 MTR_4g100810 41.606 411 217 8 77 481 104 497 7.98e-99 308
MS.gene53071.t1 MTR_3g115220 43.434 396 209 9 82 475 112 494 3.48e-98 307
MS.gene53071.t1 MTR_5g099010 44.192 396 199 9 84 474 122 500 2.62e-97 305
MS.gene53071.t1 MTR_5g012770 42.157 408 212 9 77 483 120 504 8.18e-94 294
MS.gene53071.t1 MTR_3g086570 38.776 392 213 9 76 448 145 528 1.79e-85 273
MS.gene53071.t1 MTR_5g032100 37.442 430 220 10 81 474 86 502 5.67e-85 271
MS.gene53071.t1 MTR_3g100310 36.652 442 228 11 68 473 90 515 8.47e-84 268
MS.gene53071.t1 MTR_7g064210 79.762 168 24 2 194 352 22 188 7.27e-83 263
MS.gene53071.t1 MTR_4g084570 35.024 414 246 10 81 480 150 554 6.21e-80 259
MS.gene53071.t1 MTR_5g059260 37.864 412 226 11 82 473 158 559 2.48e-79 258
MS.gene53071.t1 MTR_4g132590 37.642 441 220 11 78 473 62 492 1.02e-78 254
MS.gene53071.t1 MTR_4g132590 37.642 441 220 11 78 473 161 591 1.28e-77 254
MS.gene53071.t1 MTR_2g016470 35.520 442 236 8 75 475 183 616 5.23e-77 253
MS.gene53071.t1 MTR_1g102030 36.449 428 232 11 75 478 82 493 5.60e-77 250
MS.gene53071.t1 MTR_1g031590 34.474 409 241 9 81 473 149 546 3.85e-76 249
MS.gene53071.t1 MTR_3g005340 34.719 409 244 11 81 475 147 546 2.92e-72 239
MS.gene53071.t1 MTR_4g023030 36.386 404 232 9 84 479 236 622 1.25e-67 228
MS.gene53071.t1 MTR_3g026650 35.264 397 238 8 84 475 176 558 3.02e-67 226
MS.gene53071.t1 MTR_3g086570 38.567 293 157 7 76 350 145 432 3.73e-63 212
MS.gene53071.t1 MTR_3g026650 37.618 319 187 5 84 397 176 487 1.03e-57 199
MS.gene53071.t1 MTR_4g010990 32.603 365 224 8 81 441 75 421 3.27e-57 196
MS.gene53071.t1 MTR_1g101990 37.793 299 164 8 190 478 3 289 1.27e-55 188
MS.gene53071.t1 MTR_4g010990 31.143 350 219 8 96 441 1 332 1.48e-48 171
MS.gene53071.t1 MTR_3g092590 31.715 309 183 9 78 363 82 385 5.29e-41 155
MS.gene53071.t1 MTR_3g092590 31.715 309 183 9 78 363 82 385 1.85e-40 154
MS.gene53071.t1 MTR_7g017330 30.693 303 191 6 70 356 87 386 6.13e-39 150
MS.gene53071.t1 MTR_3g026650 37.229 231 133 5 84 309 176 399 1.15e-35 137
MS.gene53071.t1 MTR_1g007390 29.524 315 188 10 78 363 82 391 8.48e-33 132
MS.gene53071.t1 MTR_1g101985 54.217 83 38 0 75 157 82 164 1.83e-24 100
MS.gene53071.t1 MTR_4g008200 26.888 331 193 14 81 400 35 327 1.93e-23 102
MS.gene53071.t1 MTR_4g008200 26.888 331 193 14 81 400 86 378 4.17e-23 101
MS.gene53071.t1 MTR_2g038320 25.000 344 201 10 81 410 92 392 1.24e-21 97.4
MS.gene53071.t1 MTR_1g052225 35.583 163 55 6 79 241 19 131 4.78e-18 80.9
MS.gene53071.t1 MTR_6g046230 39.252 107 57 2 129 227 34 140 3.57e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53071.t1 AT1G14020 70.711 478 132 1 9 486 27 496 0.0 729
MS.gene53071.t1 AT2G03280 68.172 465 133 4 9 472 30 480 0.0 674
MS.gene53071.t1 AT2G03280 64.431 492 133 5 9 472 30 507 0.0 659
MS.gene53071.t1 AT5G15740 58.925 465 186 3 9 472 38 498 0.0 582
MS.gene53071.t1 AT3G02250 57.849 465 191 3 9 472 38 498 0.0 569
MS.gene53071.t1 AT1G22460 47.045 440 217 6 45 474 125 558 1.33e-135 403
MS.gene53071.t1 AT2G37980 47.202 411 210 5 81 489 226 631 1.76e-134 403
MS.gene53071.t1 AT3G54100 46.731 413 208 6 76 481 212 619 1.42e-132 398
MS.gene53071.t1 AT5G35570 47.355 397 202 5 80 474 250 641 2.83e-129 390
MS.gene53071.t1 AT5G01100 48.241 398 198 5 81 474 216 609 9.12e-128 385
MS.gene53071.t1 AT5G64600 46.699 409 205 6 81 480 117 521 7.70e-126 377
MS.gene53071.t1 AT4G38390 44.670 394 206 6 81 473 123 505 2.84e-103 320
MS.gene53071.t1 AT1G76270 44.162 394 204 8 84 475 109 488 5.60e-103 320
MS.gene53071.t1 AT1G20550 41.176 408 224 7 84 489 112 505 1.07e-101 316
MS.gene53071.t1 AT4G16650 44.499 409 203 10 73 474 113 504 1.72e-94 297
MS.gene53071.t1 AT4G16650 44.499 409 203 10 73 474 113 504 1.72e-94 297
MS.gene53071.t1 AT4G24530 37.674 430 219 10 81 474 101 517 2.15e-86 275
MS.gene53071.t1 AT1G04910 38.916 406 230 8 75 474 82 475 6.96e-86 274
MS.gene53071.t1 AT3G26370 38.325 394 215 9 74 448 152 536 9.31e-86 275
MS.gene53071.t1 AT1G38065 37.681 414 226 11 81 473 26 428 6.07e-83 264
MS.gene53071.t1 AT1G38065 37.681 414 226 11 81 473 36 438 8.08e-83 264
MS.gene53071.t1 AT1G62330 35.682 440 227 10 82 475 222 651 8.22e-83 269
MS.gene53071.t1 AT5G65470 35.431 429 226 10 83 473 87 502 2.62e-82 264
MS.gene53071.t1 AT1G11990 39.706 408 220 11 82 473 191 588 3.09e-82 266
MS.gene53071.t1 AT1G38131 37.681 414 226 11 81 473 155 557 1.84e-81 264
MS.gene53071.t1 AT1G11990 36.629 445 219 11 82 473 176 610 5.01e-77 253
MS.gene53071.t1 AT1G14970 35.036 411 240 10 81 475 9 408 1.45e-76 246
MS.gene53071.t1 AT1G14970 35.036 411 240 10 81 475 35 434 4.18e-76 246
MS.gene53071.t1 AT1G29200 37.644 433 223 10 81 474 70 494 4.70e-76 247
MS.gene53071.t1 AT1G29200 37.644 433 223 10 81 474 70 494 4.70e-76 247
MS.gene53071.t1 AT1G35510 35.366 410 242 9 80 475 158 558 4.80e-76 249
MS.gene53071.t1 AT5G63390 37.845 399 228 8 84 476 167 551 1.95e-75 248
MS.gene53071.t1 AT1G14970 35.036 411 240 10 81 475 155 554 5.03e-75 246
MS.gene53071.t1 AT1G29200 37.644 433 223 10 81 474 186 610 6.22e-75 248
MS.gene53071.t1 AT2G01480 35.049 408 238 10 81 472 154 550 2.68e-74 244
MS.gene53071.t1 AT1G29200 37.471 427 220 10 87 474 2 420 5.04e-74 240
MS.gene53071.t1 AT2G01480 37.255 357 205 6 81 421 154 507 6.27e-74 243
MS.gene53071.t1 AT3G07900 34.509 397 248 5 84 478 191 577 4.06e-68 229
MS.gene53071.t1 AT2G44500 34.826 402 244 8 84 480 180 568 5.88e-67 225
MS.gene53071.t1 AT1G52630 33.333 372 226 9 74 442 67 419 6.12e-59 201
MS.gene53071.t1 AT1G52630 31.445 353 214 9 96 442 1 331 7.22e-50 174
MS.gene53071.t1 AT3G03810 34.211 304 183 6 75 363 83 384 5.95e-47 173
MS.gene53071.t1 AT3G03810 34.211 304 183 6 75 363 83 384 5.95e-47 173
MS.gene53071.t1 AT3G26370 38.710 217 120 6 74 280 152 365 5.38e-43 157
MS.gene53071.t1 AT3G30300 32.558 301 180 8 81 363 100 395 3.70e-42 159
MS.gene53071.t1 AT3G30300 32.558 301 180 8 81 363 100 395 3.70e-42 159
MS.gene53071.t1 AT2G44500 36.638 232 136 5 84 310 180 405 7.54e-36 138

Find 100 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCAGATTCTGCCGCTTTA+TGG 0.087587 5.4:-31063255 MS.gene53071:intron
ACTCCTCAAATTGAGAATTT+AGG 0.103676 5.4:-31063120 MS.gene53071:CDS
TGGCATTCACATTTCATTTC+TGG 0.140172 5.4:-31065635 MS.gene53071:CDS
CTCCTCAAATTGAGAATTTA+GGG 0.207048 5.4:-31063119 MS.gene53071:CDS
ATTGCAGCTGGTGAGATTTA+TGG 0.210044 5.4:-31062125 MS.gene53071:CDS
AGCTTGATAAGACATCATTT+TGG 0.262663 5.4:-31064546 MS.gene53071:CDS
TTTGCAATTTCAGGTGAAAA+AGG 0.267125 5.4:-31061870 MS.gene53071:intron
AGGGTATACGTTTGAGGTTT+TGG 0.275369 5.4:-31065727 MS.gene53071:CDS
GTTCTTCGAGCCTTAGGTTT+TGG 0.287595 5.4:-31062167 MS.gene53071:CDS
GGCATTCACATTTCATTTCT+GGG 0.303223 5.4:-31065634 MS.gene53071:CDS
CGCCCTAAATTCTCAATTTG+AGG 0.307590 5.4:+31063117 None:intergenic
TATTGTTCTTTGGATTTGTT+TGG 0.312689 5.4:-31065684 MS.gene53071:CDS
AGAACTAAGGCCGCCTCTTC+TGG 0.312939 5.4:+31062185 None:intergenic
TGTGTTCAAGTATTGTTCTT+TGG 0.316418 5.4:-31065694 MS.gene53071:CDS
ATCATAATGGAACCCTAAAA+TGG 0.324135 5.4:-31061466 MS.gene53071:CDS
CCATCTGCGATGAGTGATTC+TGG 0.326439 5.4:+31061813 None:intergenic
AGAAATTGTGATGAGTTTCT+AGG 0.340861 5.4:-31061308 MS.gene53071:CDS
TTGATTCAAGCCACCATTGC+AGG 0.359205 5.4:+31064717 None:intergenic
CTTTCATTGGACCAGCTAAC+AGG 0.361116 5.4:+31064299 None:intergenic
CAAGTTCATGAAAAGAGAAT+TGG 0.362522 5.4:-31061419 MS.gene53071:CDS
TAGAAATAGTCCTCCTCCTT+TGG 0.381323 5.4:+31061362 None:intergenic
CTCATCGCAGATGGCTGCCT+TGG 0.387624 5.4:-31061804 MS.gene53071:CDS
ACCTTTATCCCTACTTATGA+TGG 0.388352 5.4:-31061755 MS.gene53071:CDS
AGCTAACAGGAGGCATCTCC+AGG 0.388940 5.4:+31064312 None:intergenic
CTCCAAGTAATATCTTTCAT+TGG 0.409045 5.4:+31064286 None:intergenic
GCCTTAGTTCTTCGAGCCTT+AGG 0.411617 5.4:-31062173 MS.gene53071:CDS
TGTTGACAAACTCACAAGTT+GGG 0.416079 5.4:+31062064 None:intergenic
ATGGACATGTTGGCTTTCTC+AGG 0.416770 5.4:-31063033 MS.gene53071:CDS
CCTAAAATGGCACGAGTTTG+CGG 0.417592 5.4:-31061453 MS.gene53071:CDS
GGCTTTCTCAGGTTGTACTC+AGG 0.430358 5.4:-31063022 MS.gene53071:CDS
TTCATTTCTGGGATTACTAC+TGG 0.431994 5.4:-31065623 MS.gene53071:CDS
CCCATCATAAGTAGGGATAA+AGG 0.444424 5.4:+31061754 None:intergenic
GATCAGAGGGTATACGTTTG+AGG 0.452447 5.4:-31065733 MS.gene53071:CDS
TCACCAGCTGCAATATATAT+CGG 0.453493 5.4:+31062134 None:intergenic
ATTTAGGGCGAAAATTGATT+CGG 0.453678 5.4:-31063104 MS.gene53071:CDS
TAGTGACAGTGAGTGAACTA+TGG 0.456536 5.4:-31065655 MS.gene53071:CDS
TGGAGATGCCTCCTGTTAGC+TGG 0.458292 5.4:-31064310 MS.gene53071:CDS
GCTTTCTCAGGTTGTACTCA+GGG 0.467938 5.4:-31063021 MS.gene53071:CDS
TTTCTAAGAGTTTCCTGCAA+TGG 0.482508 5.4:-31064730 MS.gene53071:CDS
TTGGTTGATATGCATCATAA+TGG 0.483990 5.4:-31061479 MS.gene53071:CDS
AGAAATGGAGGTTAGATCAG+AGG 0.484479 5.4:-31065747 None:intergenic
ACCTAAGGCTCGAAGAACTA+AGG 0.487906 5.4:+31062172 None:intergenic
AATTCAGGTATGCATTCCCT+TGG 0.488348 5.4:-31062268 MS.gene53071:intron
CTAAGAGTTTCCTGCAATGG+TGG 0.489996 5.4:-31064727 MS.gene53071:CDS
TCAGGTATGCATTCCCTTGG+TGG 0.491881 5.4:-31062265 MS.gene53071:intron
CTAAGATGCAGTGCCACAAA+AGG 0.492255 5.4:+31063063 None:intergenic
GTCCAATGAAAGATATTACT+TGG 0.492504 5.4:-31064288 MS.gene53071:CDS
ACAGGAAATTATACAAGTAA+TGG 0.492528 5.4:-31064754 MS.gene53071:intron
GCCATGTTCCCATCATAAGT+AGG 0.492805 5.4:+31061746 None:intergenic
AATGATGTCTTATCAAGCTC+TGG 0.501078 5.4:+31064550 None:intergenic
AGGAGATAATTTCTGAAGAG+AGG 0.503455 5.4:-31062235 MS.gene53071:CDS
TAGATATGAGATGGACATGT+TGG 0.506081 5.4:-31063043 MS.gene53071:CDS
CCATGTTCCCATCATAAGTA+GGG 0.511468 5.4:+31061747 None:intergenic
GAAATGGAGGTTAGATCAGA+GGG 0.517232 5.4:-31065746 MS.gene53071:CDS
ATTCCCTTGGTGGAGAGAAA+AGG 0.524582 5.4:-31062255 MS.gene53071:CDS
GCCGCCTCTTCTGGTGTCAA+AGG 0.536521 5.4:+31062194 None:intergenic
GATTCGGATGCTTCACGAGA+AGG 0.536968 5.4:-31063088 MS.gene53071:CDS
CCTTTATCCCTACTTATGAT+GGG 0.537062 5.4:-31061754 MS.gene53071:CDS
AGTAGGGATAAAGGTGTCAC+TGG 0.538681 5.4:+31061763 None:intergenic
TTCTGAAGAGAGGAGATCAC+AGG 0.544928 5.4:-31062225 MS.gene53071:CDS
TGAAAGATATTACTTGGAGC+AGG 0.549066 5.4:-31064282 MS.gene53071:intron
CCCTACTTATGATGGGAACA+TGG 0.557915 5.4:-31061747 MS.gene53071:CDS
CTGAGTTTCTGTAGATCAAG+TGG 0.559172 5.4:+31063174 None:intergenic
ACCTTGTGCTTTCCATAAAG+CGG 0.559211 5.4:+31063243 None:intergenic
ATTCGGATGCTTCACGAGAA+GGG 0.561013 5.4:-31063087 MS.gene53071:CDS
CCAGAATCACTCATCGCAGA+TGG 0.564959 5.4:-31061813 MS.gene53071:CDS
CCGAGGAACTCAAAAGATTG+AGG 0.570538 5.4:-31062981 MS.gene53071:intron
TGGGAACATGGCAAAACTTG+TGG 0.580054 5.4:-31061735 MS.gene53071:CDS
CACTTGATCTACAGAAACTC+AGG 0.581030 5.4:-31063173 MS.gene53071:CDS
ACACCGATATATATTGCAGC+TGG 0.586158 5.4:-31062137 MS.gene53071:CDS
TCCTTTGACACCAGAAGAGG+CGG 0.587100 5.4:-31062195 MS.gene53071:CDS
GACGCACGTCTAGCAAATAA+CGG 0.591633 5.4:-31063201 MS.gene53071:CDS
TGTCTCCCTACCAAAACCTA+AGG 0.604346 5.4:+31062157 None:intergenic
TACTGAAACCATAAAATCCA+AGG 0.605425 5.4:+31061787 None:intergenic
AGATTTATGGCGGTGAACGT+AGG 0.606142 5.4:-31062112 MS.gene53071:CDS
TATAATCGACAGACCAAAGG+AGG 0.606405 5.4:-31061375 MS.gene53071:CDS
TCCTAAACAAAATAATGTCG+TGG 0.607462 5.4:-31065573 MS.gene53071:CDS
AACTTGTGGAAGGCCATCGA+AGG 0.608718 5.4:-31061721 MS.gene53071:intron
AACATGGCAAAACTTGTGGA+AGG 0.609196 5.4:-31061731 MS.gene53071:CDS
CCCTCATGAGTGTCTATGTG+AGG 0.609839 5.4:-31061333 MS.gene53071:CDS
GTTGACAAACTCACAAGTTG+GGG 0.614088 5.4:+31062065 None:intergenic
AAATGGCACGAGTTTGCGGA+TGG 0.616122 5.4:-31061449 MS.gene53071:CDS
ATGTCCTTTGACACCAGAAG+AGG 0.616187 5.4:-31062198 MS.gene53071:CDS
TTGCTCAGAGAAAGAAGCCG+AGG 0.622924 5.4:-31062998 MS.gene53071:CDS
CTTGAATCAAATGCGTGCTG+CGG 0.623967 5.4:-31064704 MS.gene53071:intron
AGTTATAATCGACAGACCAA+AGG 0.626511 5.4:-31061378 MS.gene53071:CDS
CCTCACATAGACACTCATGA+GGG 0.628028 5.4:+31061333 None:intergenic
ACTGCATCTTAGATATGAGA+TGG 0.633287 5.4:-31063052 MS.gene53071:CDS
GGGTAAATACATACCTTCGA+TGG 0.636072 5.4:+31061708 None:intergenic
AATCGACAGACCAAAGGAGG+AGG 0.642909 5.4:-31061372 MS.gene53071:CDS
TAAATATGGATATTCTACCC+TGG 0.644817 5.4:-31064330 MS.gene53071:CDS
GCCGCTTTATGGAAAGCACA+AGG 0.648292 5.4:-31063244 MS.gene53071:CDS
TGAGTTTCTGTAGATCAAGT+GGG 0.659206 5.4:+31063175 None:intergenic
TCATTGGACCAGCTAACAGG+AGG 0.662833 5.4:+31064302 None:intergenic
TCCTCACATAGACACTCATG+AGG 0.668777 5.4:+31061332 None:intergenic
GCTAACAGGAGGCATCTCCA+GGG 0.682429 5.4:+31064313 None:intergenic
CAGGTGTCGTGTCAATTACG+AGG 0.683646 5.4:-31063154 MS.gene53071:CDS
TCTGAAGAGAGGAGATCACA+GGG 0.690255 5.4:-31062224 MS.gene53071:CDS
GCAGCTGGTGAGATTTATGG+CGG 0.732613 5.4:-31062122 MS.gene53071:CDS
GGTATTTCAGATATGTGACA+TGG 0.740956 5.4:-31064611 MS.gene53071:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATTATTTAGAGATATTA+AGG - chr5.4:31064269-31064288 MS.gene53071:intron 10.0%
!! AAATATCATTTACTAATATG+AGG + chr5.4:31064185-31064204 None:intergenic 15.0%
!! AAATTAGTTAGAAATTAAAG+AGG + chr5.4:31065463-31065482 None:intergenic 15.0%
!! AATACTAAAAACACAATAAA+TGG + chr5.4:31064519-31064538 None:intergenic 15.0%
!! ATAAATCAATGTTCAATAAA+AGG - chr5.4:31061985-31062004 MS.gene53071:intron 15.0%
!! ATGAAGAAATTTATAGTTAT+GGG - chr5.4:31063546-31063565 MS.gene53071:intron 15.0%
!! GATCAATTAATTTATATCAA+AGG + chr5.4:31061591-31061610 None:intergenic 15.0%
!! GGGAAAAAAAAAAAAAAAAA+AGG + chr5.4:31063311-31063330 None:intergenic 15.0%
!! TAAATCAATGTTCAATAAAA+GGG - chr5.4:31061986-31062005 MS.gene53071:intron 15.0%
!! TGGAATAAAATAGAATTATA+GGG - chr5.4:31063074-31063093 MS.gene53071:CDS 15.0%
!!! AAACAGATAAATTTTAGAAT+AGG + chr5.4:31062357-31062376 None:intergenic 15.0%
!! AAAAACCAAGTTTACTAAAT+AGG + chr5.4:31063592-31063611 None:intergenic 20.0%
!! AAAAAGTTTAGCAGTAAATA+TGG - chr5.4:31062682-31062701 MS.gene53071:intron 20.0%
!! AAAACATATCTGATACAAAA+AGG + chr5.4:31061541-31061560 None:intergenic 20.0%
!! AAGTCAATTATATGTTTGAA+AGG + chr5.4:31063332-31063351 None:intergenic 20.0%
!! AGCATATAATCAAGTAAATA+AGG + chr5.4:31064625-31064644 None:intergenic 20.0%
!! AGTCAATTATATGTTTGAAA+GGG + chr5.4:31063331-31063350 None:intergenic 20.0%
!! ATTATTGTCAATACATTAGA+GGG - chr5.4:31065388-31065407 MS.gene53071:intron 20.0%
!! ATTGAAAAATTAACAGTTTG+GGG + chr5.4:31062900-31062919 None:intergenic 20.0%
!! CATGAAGAAATTTATAGTTA+TGG - chr5.4:31063545-31063564 MS.gene53071:intron 20.0%
!! CATTGAAAAATTAACAGTTT+GGG + chr5.4:31062901-31062920 None:intergenic 20.0%
!! GCATATAATCAAGTAAATAA+GGG + chr5.4:31064624-31064643 None:intergenic 20.0%
!! GTGGAATAAAATAGAATTAT+AGG - chr5.4:31063073-31063092 MS.gene53071:CDS 20.0%
!! TATTATTGTCAATACATTAG+AGG - chr5.4:31065387-31065406 MS.gene53071:intron 20.0%
!! TTATTACACAAATTTCACTT+TGG + chr5.4:31065415-31065434 None:intergenic 20.0%
!!! CAAGATTGTTTTTCTAAAAT+CGG + chr5.4:31065503-31065522 None:intergenic 20.0%
!!! GTATTTTTATGTTTCATGTT+TGG - chr5.4:31064067-31064086 MS.gene53071:intron 20.0%
!!! GTTGGTAAATTTTGTTTTTT+TGG - chr5.4:31061714-31061733 MS.gene53071:intron 20.0%
!!! TATTTACTGCTAAACTTTTT+AGG + chr5.4:31062682-31062701 None:intergenic 20.0%
!!! TTCTAACTATTTGCATTTTA+AGG - chr5.4:31062815-31062834 MS.gene53071:intron 20.0%
!!! TTTTAGAAAAACAATCTTGT+TGG - chr5.4:31065504-31065523 MS.gene53071:intron 20.0%
!!! TTTTATTTTCTCACTCATTT+TGG - chr5.4:31062218-31062237 MS.gene53071:CDS 20.0%
! AAAATCAGTGTTATCAATAG+TGG + chr5.4:31063447-31063466 None:intergenic 25.0%
! AAGCTTAAATACTCATTTAG+TGG - chr5.4:31062389-31062408 MS.gene53071:intron 25.0%
! AAGTCTATGAAAACAAGAAA+TGG + chr5.4:31064376-31064395 None:intergenic 25.0%
! AATAACAGTAAGTGCATTAT+TGG - chr5.4:31065430-31065449 MS.gene53071:intron 25.0%
! AATAGGTCATGTTCTTTAAA+AGG + chr5.4:31063216-31063235 None:intergenic 25.0%
! AATTTCAAAGACTGAAAATG+AGG + chr5.4:31065106-31065125 None:intergenic 25.0%
! ACAGGAAATTATACAAGTAA+TGG - chr5.4:31062272-31062291 MS.gene53071:intron 25.0%
! AGTGTAAATTGTATTTGTGT+TGG - chr5.4:31061696-31061715 MS.gene53071:intron 25.0%
! ATACCTATCCTCAAAATTAA+TGG + chr5.4:31062125-31062144 None:intergenic 25.0%
! ATAGATATCTAATTGCTGTA+AGG + chr5.4:31064683-31064702 None:intergenic 25.0%
! ATGTTGCAGACAAATAAAAA+GGG + chr5.4:31063287-31063306 None:intergenic 25.0%
! GAGATGTACCTTATTAAAAA+GGG + chr5.4:31062025-31062044 None:intergenic 25.0%
! GCATTGAAAAATTAACAGTT+TGG + chr5.4:31062902-31062921 None:intergenic 25.0%
! TAGATATCTAATTGCTGTAA+GGG + chr5.4:31064682-31064701 None:intergenic 25.0%
! TGAGATGTACCTTATTAAAA+AGG + chr5.4:31062026-31062045 None:intergenic 25.0%
! TTACTTGTATAATTTCCTGT+AGG + chr5.4:31062272-31062291 None:intergenic 25.0%
! TTTCAAATTATAATAACCCC+AGG + chr5.4:31061846-31061865 None:intergenic 25.0%
!! CAGTTTTCTAATAAGACAAT+AGG + chr5.4:31063150-31063169 None:intergenic 25.0%
!! CTGATTTTCAAAGAAGTTAT+GGG - chr5.4:31063459-31063478 MS.gene53071:intron 25.0%
!! GTTTTCCTATTTAGTAAACT+TGG - chr5.4:31063584-31063603 MS.gene53071:intron 25.0%
!! TATTAAAGCCCTTTTACTTT+TGG - chr5.4:31064439-31064458 MS.gene53071:intron 25.0%
!! TATTGTGATTTAAGATTCTG+TGG - chr5.4:31064654-31064673 MS.gene53071:intron 25.0%
!! TATTGTTCTTTGGATTTGTT+TGG - chr5.4:31061342-31061361 MS.gene53071:CDS 25.0%
!! TGATTCTTGACCTATATTTT+TGG - chr5.4:31062861-31062880 MS.gene53071:intron 25.0%
!! TTCTTTTTGCAGTAATTTTG+AGG - chr5.4:31062591-31062610 MS.gene53071:intron 25.0%
!! TTTTTAATAAGGTACATCTC+AGG - chr5.4:31062025-31062044 MS.gene53071:intron 25.0%
!!! AACATGGCTTATATTTTGAA+AGG + chr5.4:31063254-31063273 None:intergenic 25.0%
!!! AATTTGAAATGCTGATTCTA+GGG - chr5.4:31061857-31061876 MS.gene53071:CDS 25.0%
!!! ACCTATATTTTTGGATAGTA+TGG - chr5.4:31062870-31062889 MS.gene53071:intron 25.0%
!!! ACTGATTTTCAAAGAAGTTA+TGG - chr5.4:31063458-31063477 MS.gene53071:intron 25.0%
!!! ATTAAAGCCCTTTTACTTTT+GGG - chr5.4:31064440-31064459 MS.gene53071:intron 25.0%
!!! CAACTAAAATAAGTTGCTTT+TGG - chr5.4:31063614-31063633 MS.gene53071:intron 25.0%
!!! CTGTTTTTATTTTCCTTCTA+CGG - chr5.4:31064317-31064336 MS.gene53071:CDS 25.0%
!!! CTTGTTTTAATGTTGAATTG+TGG - chr5.4:31061744-31061763 MS.gene53071:CDS 25.0%
!!! TAATTTGAAATGCTGATTCT+AGG - chr5.4:31061856-31061875 MS.gene53071:CDS 25.0%
!!! TACACAACTTTAGAATGATA+TGG + chr5.4:31062568-31062587 None:intergenic 25.0%
!!! TTAATTTTGAGGATAGGTAT+AGG - chr5.4:31062125-31062144 MS.gene53071:CDS 25.0%
!!! TTTTGAAAGGCTTACATATA+TGG + chr5.4:31063241-31063260 None:intergenic 25.0%
AAACGTTTCCCAAAAGTAAA+AGG + chr5.4:31064451-31064470 None:intergenic 30.0%
AACGTTTCCCAAAAGTAAAA+GGG + chr5.4:31064450-31064469 None:intergenic 30.0%
AATAACATGTCAGCAACATT+CGG + chr5.4:31065342-31065361 None:intergenic 30.0%
AATCTTGTTGGATCGAAAAA+AGG - chr5.4:31065516-31065535 MS.gene53071:intron 30.0%
ACTCCTCAAATTGAGAATTT+AGG - chr5.4:31063906-31063925 MS.gene53071:intron 30.0%
AGAAATTGTGATGAGTTTCT+AGG - chr5.4:31065718-31065737 MS.gene53071:CDS 30.0%
ATAAGACAATAGGTCATGTT+TGG + chr5.4:31063140-31063159 None:intergenic 30.0%
CAAGTTCATGAAAAGAGAAT+TGG - chr5.4:31065607-31065626 MS.gene53071:CDS 30.0%
CAATATTGCTTATCGATGAA+TGG - chr5.4:31062156-31062175 MS.gene53071:CDS 30.0%
CAGAACAAAGTATATACCTA+GGG + chr5.4:31062511-31062530 None:intergenic 30.0%
CATGTTGCAGACAAATAAAA+AGG + chr5.4:31063288-31063307 None:intergenic 30.0%
CTATCCTAGTTTCGTTATTA+GGG - chr5.4:31063002-31063021 MS.gene53071:CDS 30.0%
CTATCTTGTATTTCACATAG+CGG - chr5.4:31063392-31063411 MS.gene53071:intron 30.0%
CTCCAAGTAATATCTTTCAT+TGG + chr5.4:31062743-31062762 None:intergenic 30.0%
CTCCTCAAATTGAGAATTTA+GGG - chr5.4:31063907-31063926 MS.gene53071:intron 30.0%
CTTGGAGGAAGAAAAATAAT+CGG + chr5.4:31061493-31061512 None:intergenic 30.0%
GAAATACAAGATAGCATTGT+GGG + chr5.4:31063386-31063405 None:intergenic 30.0%
GACTTATATTAGGTGTTAGA+TGG - chr5.4:31061648-31061667 MS.gene53071:intron 30.0%
GCATACCTGAATTACAAAAA+GGG + chr5.4:31064751-31064770 None:intergenic 30.0%
GCCATACTATCCAAAAATAT+AGG + chr5.4:31062874-31062893 None:intergenic 30.0%
GTCCAATGAAAGATATTACT+TGG - chr5.4:31062738-31062757 MS.gene53071:intron 30.0%
TAAATACTCATTTAGTGGCA+TGG - chr5.4:31062394-31062413 MS.gene53071:intron 30.0%
TAAATATGGATATTCTACCC+TGG - chr5.4:31062696-31062715 MS.gene53071:intron 30.0%
TACTGAAACCATAAAATCCA+AGG + chr5.4:31065242-31065261 None:intergenic 30.0%
TCAAACAATGAGCAGAAAAA+TGG + chr5.4:31065001-31065020 None:intergenic 30.0%
TCAGAACAAAGTATATACCT+AGG + chr5.4:31062512-31062531 None:intergenic 30.0%
TCCTAAACAAAATAATGTCG+TGG - chr5.4:31061453-31061472 MS.gene53071:CDS 30.0%
TGAAATACAAGATAGCATTG+TGG + chr5.4:31063387-31063406 None:intergenic 30.0%
TGATATGGAGGTTAAATTAG+AGG + chr5.4:31062553-31062572 None:intergenic 30.0%
TGCATACCTGAATTACAAAA+AGG + chr5.4:31064752-31064771 None:intergenic 30.0%
TTAGAGTCAAGAAACTAACT+TGG + chr5.4:31061511-31061530 None:intergenic 30.0%
TTCTGTAATTGAGGATAAGA+GGG - chr5.4:31064121-31064140 MS.gene53071:intron 30.0%
! AGCTTGATAAGACATCATTT+TGG - chr5.4:31062480-31062499 MS.gene53071:intron 30.0%
! ATCATAATGGAACCCTAAAA+TGG - chr5.4:31065560-31065579 MS.gene53071:CDS 30.0%
! ATTATAACTTTTCGACGAGT+TGG + chr5.4:31065637-31065656 None:intergenic 30.0%
! ATTTAGGGCGAAAATTGATT+CGG - chr5.4:31063922-31063941 MS.gene53071:intron 30.0%
! CTTATTAAAAAGGGCATATC+CGG + chr5.4:31062016-31062035 None:intergenic 30.0%
! GCTTGATAAGACATCATTTT+GGG - chr5.4:31062481-31062500 MS.gene53071:intron 30.0%
! GGTGGATTTGTAATTCAAAA+AGG - chr5.4:31064554-31064573 MS.gene53071:CDS 30.0%
! GGTGTTATGTGACTTATATT+AGG - chr5.4:31061638-31061657 MS.gene53071:intron 30.0%
! TGTGTTCAAGTATTGTTCTT+TGG - chr5.4:31061332-31061351 MS.gene53071:CDS 30.0%
! TGTTAAGTGTTGTTCTATAG+TGG - chr5.4:31061807-31061826 MS.gene53071:CDS 30.0%
! TTGGTTGATATGCATCATAA+TGG - chr5.4:31065547-31065566 MS.gene53071:CDS 30.0%
! TTTGCAATTTCAGGTGAAAA+AGG - chr5.4:31065156-31065175 MS.gene53071:intron 30.0%
!! AAGTGTTGCCATTAATTTTG+AGG - chr5.4:31062114-31062133 MS.gene53071:CDS 30.0%
!! ACAACTTTAGAATGATATGG+AGG + chr5.4:31062565-31062584 None:intergenic 30.0%
!! ACCACGACATTATTTTGTTT+AGG + chr5.4:31061457-31061476 None:intergenic 30.0%
!! GCTTTTGTGTATTGTTGATT+AGG - chr5.4:31061617-31061636 MS.gene53071:intron 30.0%
!! TAGTGGATTGCAGAAAATAA+AGG + chr5.4:31063430-31063449 None:intergenic 30.0%
!! TTCTAAAGTTCCAATCAAGT+TGG + chr5.4:31063501-31063520 None:intergenic 30.0%
!! TTGCCATTAATTTTGAGGAT+AGG - chr5.4:31062119-31062138 MS.gene53071:CDS 30.0%
!!! AAATAAGTTGCTTTTGGTGT+AGG - chr5.4:31063620-31063639 MS.gene53071:intron 30.0%
!!! CTATTTTTTGGAACCTATGT+TGG + chr5.4:31063650-31063669 None:intergenic 30.0%
!!! TATTTCACATAGCGGATTTT+TGG - chr5.4:31063400-31063419 MS.gene53071:intron 30.0%
AAAGGGAATAAAGCAGAACA+TGG + chr5.4:31063270-31063289 None:intergenic 35.0%
AATGATGTCTTATCAAGCTC+TGG + chr5.4:31062479-31062498 None:intergenic 35.0%
AATTGAGGATAAGAGGGATT+TGG - chr5.4:31064127-31064146 MS.gene53071:intron 35.0%
ACAATGAGCAGAAAAATGGA+AGG + chr5.4:31064997-31065016 None:intergenic 35.0%
ACTGCATCTTAGATATGAGA+TGG - chr5.4:31063974-31063993 MS.gene53071:intron 35.0%
AGTATTATCTCTTGTACACG+TGG - chr5.4:31064533-31064552 MS.gene53071:intron 35.0%
AGTTATAATCGACAGACCAA+AGG - chr5.4:31065648-31065667 MS.gene53071:CDS 35.0%
ATAACATGTCAGCAACATTC+GGG + chr5.4:31065341-31065360 None:intergenic 35.0%
ATAATTTCCTGTAGGTTGTC+AGG + chr5.4:31062264-31062283 None:intergenic 35.0%
ATGTTGACAAACTCACAAGT+TGG + chr5.4:31064966-31064985 None:intergenic 35.0%
ATTGTTCTTGCGCTCATTAA+TGG - chr5.4:31065042-31065061 MS.gene53071:intron 35.0%
CATTCTTGCTTCTGTAATTG+AGG - chr5.4:31064112-31064131 MS.gene53071:intron 35.0%
CTTCTGTAATTGAGGATAAG+AGG - chr5.4:31064120-31064139 MS.gene53071:intron 35.0%
CTTTCTGTGTTTGCAATTTC+AGG - chr5.4:31065147-31065166 MS.gene53071:intron 35.0%
GAAAATGCATCTTTCAGATC+AGG + chr5.4:31065073-31065092 None:intergenic 35.0%
GACTTATTTCAACTGCTATG+CGG - chr5.4:31063347-31063366 MS.gene53071:intron 35.0%
GCTATCCTAGTTTCGTTATT+AGG - chr5.4:31063001-31063020 MS.gene53071:CDS 35.0%
GGCATTCACATTTCATTTCT+GGG - chr5.4:31061392-31061411 MS.gene53071:CDS 35.0%
GGCTTACATATATGGTGAAT+AGG + chr5.4:31063233-31063252 None:intergenic 35.0%
GGTATTTCAGATATGTGACA+TGG - chr5.4:31062415-31062434 MS.gene53071:intron 35.0%
TAGATATGAGATGGACATGT+TGG - chr5.4:31063983-31064002 MS.gene53071:intron 35.0%
TCACCAGCTGCAATATATAT+CGG + chr5.4:31064895-31064914 None:intergenic 35.0%
TGAAAGATATTACTTGGAGC+AGG - chr5.4:31062744-31062763 MS.gene53071:intron 35.0%
TGAGTTTCTGTAGATCAAGT+GGG + chr5.4:31063854-31063873 None:intergenic 35.0%
TGGCATTCACATTTCATTTC+TGG - chr5.4:31061391-31061410 MS.gene53071:CDS 35.0%
TGTTCAATAAAAGGGATCTC+CGG - chr5.4:31061994-31062013 MS.gene53071:intron 35.0%
TGTTGACAAACTCACAAGTT+GGG + chr5.4:31064965-31064984 None:intergenic 35.0%
TTGAATCAACTAGCAGAAAC+TGG + chr5.4:31063045-31063064 None:intergenic 35.0%
TTGTAACGAAAAACCGTAGA+AGG + chr5.4:31064333-31064352 None:intergenic 35.0%
! ACTGTCCCTTTTTGTAATTC+AGG - chr5.4:31064743-31064762 MS.gene53071:CDS 35.0%
! AGGAGATAATTTCTGAAGAG+AGG - chr5.4:31064791-31064810 MS.gene53071:intron 35.0%
! AGTTGATTCAATGGCAATAG+TGG - chr5.4:31063054-31063073 MS.gene53071:CDS 35.0%
! CGGATATGCCCTTTTTAATA+AGG - chr5.4:31062014-31062033 MS.gene53071:intron 35.0%
! GTTTCTGCTAGTTGATTCAA+TGG - chr5.4:31063045-31063064 MS.gene53071:CDS 35.0%
! TGTGGATTGTGAAATCTGTT+GGG - chr5.4:31061762-31061781 MS.gene53071:CDS 35.0%
! TTGTGGATTGTGAAATCTGT+TGG - chr5.4:31061761-31061780 MS.gene53071:CDS 35.0%
! TTTCTAAGAGTTTCCTGCAA+TGG - chr5.4:31062296-31062315 MS.gene53071:intron 35.0%
!! AAGTTCCAATCAAGTTGGAA+AGG + chr5.4:31063496-31063515 None:intergenic 35.0%
!! TAACTAATTTGTTCCTCTGC+AGG - chr5.4:31065473-31065492 MS.gene53071:intron 35.0%
!! TTATCGATGAATGGTTCTGA+TGG - chr5.4:31062165-31062184 MS.gene53071:CDS 35.0%
!! TTCATTTCTGGGATTACTAC+TGG - chr5.4:31061403-31061422 MS.gene53071:CDS 35.0%
!!! AGTGGTTTTTGAAATTGTCC+TGG - chr5.4:31061825-31061844 MS.gene53071:CDS 35.0%
!!! GTGGTTTTTGAAATTGTCCT+GGG - chr5.4:31061826-31061845 MS.gene53071:CDS 35.0%
!!! TGGTTTTTGAAATTGTCCTG+GGG - chr5.4:31061827-31061846 MS.gene53071:CDS 35.0%
AACATGGCAAAACTTGTGGA+AGG - chr5.4:31065295-31065314 MS.gene53071:intron 40.0%
AAGAGGGATTTGGATCTTCT+CGG - chr5.4:31064137-31064156 MS.gene53071:intron 40.0%
AATTCAGGTATGCATTCCCT+TGG - chr5.4:31064758-31064777 MS.gene53071:intron 40.0%
ACACCGATATATATTGCAGC+TGG - chr5.4:31064889-31064908 MS.gene53071:intron 40.0%
ACCTTGTGCTTTCCATAAAG+CGG + chr5.4:31063786-31063805 None:intergenic 40.0%
ACTAGCAGAAACTGGAATAG+CGG + chr5.4:31063037-31063056 None:intergenic 40.0%
ACTGCCCTAATAACGAAACT+AGG + chr5.4:31063009-31063028 None:intergenic 40.0%
ATAGCATTGTGGGCAAATAG+TGG + chr5.4:31063376-31063395 None:intergenic 40.0%
ATTATCTCTTGTACACGTGG+TGG - chr5.4:31064536-31064555 MS.gene53071:CDS 40.0%
ATTGCAGCTGGTGAGATTTA+TGG - chr5.4:31064901-31064920 MS.gene53071:intron 40.0%
CAAGAATGAATTAGAAGCCG+AGG + chr5.4:31064100-31064119 None:intergenic 40.0%
CACTTGATCTACAGAAACTC+AGG - chr5.4:31063853-31063872 MS.gene53071:intron 40.0%
CAGTGTAGAGTCAATAGTGT+TGG + chr5.4:31063754-31063773 None:intergenic 40.0%
CGCCCTAAATTCTCAATTTG+AGG + chr5.4:31063912-31063931 None:intergenic 40.0%
CTGAGTTTCTGTAGATCAAG+TGG + chr5.4:31063855-31063874 None:intergenic 40.0%
CTTCTCGGTTTCTGAATAAC+AGG - chr5.4:31064152-31064171 MS.gene53071:intron 40.0%
GAGTCAAGAAACTAACTTGG+AGG + chr5.4:31061508-31061527 None:intergenic 40.0%
GGAAGAAAAATAATCGGAGG+CGG + chr5.4:31061487-31061506 None:intergenic 40.0%
GGAGGAAGAAAAATAATCGG+AGG + chr5.4:31061490-31061509 None:intergenic 40.0%
GGGTAAATACATACCTTCGA+TGG + chr5.4:31065321-31065340 None:intergenic 40.0%
GTTGACAAACTCACAAGTTG+GGG + chr5.4:31064964-31064983 None:intergenic 40.0%
TAAAATCGGAATACCTGCAG+AGG + chr5.4:31065489-31065508 None:intergenic 40.0%
TAGAAATAGTCCTCCTCCTT+TGG + chr5.4:31065667-31065686 None:intergenic 40.0%
TAGTGACAGTGAGTGAACTA+TGG - chr5.4:31061371-31061390 MS.gene53071:CDS 40.0%
TATAATCGACAGACCAAAGG+AGG - chr5.4:31065651-31065670 MS.gene53071:CDS 40.0%
TCGAAAAAAGGTCGTGGAAT+TGG - chr5.4:31065528-31065547 MS.gene53071:intron 40.0%
TGTGTCCTTTCCAACTTGAT+TGG - chr5.4:31063488-31063507 MS.gene53071:intron 40.0%
TTCTTGCGCTCATTAATGGT+TGG - chr5.4:31065046-31065065 MS.gene53071:intron 40.0%
! GCTCTGATAGAGAAACCTTT+TGG - chr5.4:31064211-31064230 MS.gene53071:intron 40.0%
!! AGGGTATACGTTTGAGGTTT+TGG - chr5.4:31061299-31061318 MS.gene53071:CDS 40.0%
!! GATGAATGGTTCTGATGGAT+AGG - chr5.4:31062170-31062189 MS.gene53071:CDS 40.0%
!!! CCTCAATCTTTTGAGTTCCT+CGG + chr5.4:31064048-31064067 None:intergenic 40.0%
!!! CTCAGCGAAGTGCTATTTTT+TGG + chr5.4:31063662-31063681 None:intergenic 40.0%
ACCTAAGGCTCGAAGAACTA+AGG + chr5.4:31064857-31064876 None:intergenic 45.0%
AGATTTATGGCGGTGAACGT+AGG - chr5.4:31064914-31064933 MS.gene53071:intron 45.0%
ATGAATTAGAAGCCGAGGCA+TGG + chr5.4:31064095-31064114 None:intergenic 45.0%
ATGTCCTTTGACACCAGAAG+AGG - chr5.4:31064828-31064847 MS.gene53071:intron 45.0%
ATTCCCTTGGTGGAGAGAAA+AGG - chr5.4:31064771-31064790 MS.gene53071:intron 45.0%
ATTCGGATGCTTCACGAGAA+GGG - chr5.4:31063939-31063958 MS.gene53071:intron 45.0%
CCACTACTCTCGTATCCAAA+AGG + chr5.4:31064229-31064248 None:intergenic 45.0%
CCGAGGAACTCAAAAGATTG+AGG - chr5.4:31064045-31064064 MS.gene53071:intron 45.0%
CCTAAAATGGCACGAGTTTG+CGG - chr5.4:31065573-31065592 MS.gene53071:CDS 45.0%
CCTCACATAGACACTCATGA+GGG + chr5.4:31065696-31065715 None:intergenic 45.0%
CTAAGAGTTTCCTGCAATGG+TGG - chr5.4:31062299-31062318 MS.gene53071:intron 45.0%
CTAAGATGCAGTGCCACAAA+AGG + chr5.4:31063966-31063985 None:intergenic 45.0%
CTTGAATCAAATGCGTGCTG+CGG - chr5.4:31062322-31062341 MS.gene53071:intron 45.0%
CTTTCATTGGACCAGCTAAC+AGG + chr5.4:31062730-31062749 None:intergenic 45.0%
GACGCACGTCTAGCAAATAA+CGG - chr5.4:31063825-31063844 MS.gene53071:intron 45.0%
GCACTGTTGTCAAATAGCAG+CGG - chr5.4:31062928-31062947 MS.gene53071:intron 45.0%
GTTGGATCGAAAAAAGGTCG+TGG - chr5.4:31065522-31065541 MS.gene53071:intron 45.0%
TCCTCACATAGACACTCATG+AGG + chr5.4:31065697-31065716 None:intergenic 45.0%
TCTGAAGAGAGGAGATCACA+GGG - chr5.4:31064802-31064821 MS.gene53071:intron 45.0%
TCTTTGACCTGACAACCTAC+AGG - chr5.4:31062254-31062273 MS.gene53071:CDS 45.0%
TGGGAACATGGCAAAACTTG+TGG - chr5.4:31065291-31065310 MS.gene53071:intron 45.0%
TGGTGTAGGATAGCCAACAT+AGG - chr5.4:31063634-31063653 MS.gene53071:intron 45.0%
TGTCTCCCTACCAAAACCTA+AGG + chr5.4:31064872-31064891 None:intergenic 45.0%
TTGATTCAAGCCACCATTGC+AGG + chr5.4:31062312-31062331 None:intergenic 45.0%
! ATCTCCTTTTCTCTCCACCA+AGG + chr5.4:31064778-31064797 None:intergenic 45.0%
! CCTTTTGGATACGAGAGTAG+TGG - chr5.4:31064226-31064245 MS.gene53071:intron 45.0%
! GATCAGAGGGTATACGTTTG+AGG - chr5.4:31061293-31061312 MS.gene53071:CDS 45.0%
! GATGGCTGCCTTGGATTTTA+TGG - chr5.4:31065231-31065250 MS.gene53071:intron 45.0%
! GCTTTCTCAGGTTGTACTCA+GGG - chr5.4:31064005-31064024 MS.gene53071:intron 45.0%
! GTTCTTCGAGCCTTAGGTTT+TGG - chr5.4:31064859-31064878 MS.gene53071:intron 45.0%
! TCTCCTTTTCTCTCCACCAA+GGG + chr5.4:31064777-31064796 None:intergenic 45.0%
! TTCTGAAGAGAGGAGATCAC+AGG - chr5.4:31064801-31064820 MS.gene53071:intron 45.0%
!! ATGGACATGTTGGCTTTCTC+AGG - chr5.4:31063993-31064012 MS.gene53071:intron 45.0%
!! TCGAGCCTTAGGTTTTGGTA+GGG - chr5.4:31064864-31064883 MS.gene53071:intron 45.0%
!! TTCGAGCCTTAGGTTTTGGT+AGG - chr5.4:31064863-31064882 MS.gene53071:intron 45.0%
!!! CGCAAACTCGTGCCATTTTA+GGG + chr5.4:31065575-31065594 None:intergenic 45.0%
!! GTATTATAATTAAATAATAA+CGG - chr5.4:31061903-31061922 MS.gene53071:intron 5.0%
AAATGGCACGAGTTTGCGGA+TGG - chr5.4:31065577-31065596 MS.gene53071:CDS 50.0%
AACTTGTGGAAGGCCATCGA+AGG - chr5.4:31065305-31065324 MS.gene53071:intron 50.0%
AATCGACAGACCAAAGGAGG+AGG - chr5.4:31065654-31065673 MS.gene53071:CDS 50.0%
CCAGAATCACTCATCGCAGA+TGG - chr5.4:31065213-31065232 MS.gene53071:intron 50.0%
CCATCTGCGATGAGTGATTC+TGG + chr5.4:31065216-31065235 None:intergenic 50.0%
CCCTCATGAGTGTCTATGTG+AGG - chr5.4:31065693-31065712 MS.gene53071:CDS 50.0%
GATTCGGATGCTTCACGAGA+AGG - chr5.4:31063938-31063957 MS.gene53071:intron 50.0%
GCAGCTGGTGAGATTTATGG+CGG - chr5.4:31064904-31064923 MS.gene53071:intron 50.0%
TCAGGTATGCATTCCCTTGG+TGG - chr5.4:31064761-31064780 MS.gene53071:intron 50.0%
TCATTGGACCAGCTAACAGG+AGG + chr5.4:31062727-31062746 None:intergenic 50.0%
TCCTTTGACACCAGAAGAGG+CGG - chr5.4:31064831-31064850 MS.gene53071:intron 50.0%
TTGCTCAGAGAAAGAAGCCG+AGG - chr5.4:31064028-31064047 MS.gene53071:intron 50.0%
! CAGGTGTCGTGTCAATTACG+AGG - chr5.4:31063872-31063891 MS.gene53071:intron 50.0%
! CATCATTTTGGGCAGACCCT+AGG - chr5.4:31062492-31062511 MS.gene53071:intron 50.0%
! GCCGCTTTATGGAAAGCACA+AGG - chr5.4:31063782-31063801 MS.gene53071:intron 50.0%
! GCCTTAGTTCTTCGAGCCTT+AGG - chr5.4:31064853-31064872 MS.gene53071:intron 50.0%
! GGCTTTCTCAGGTTGTACTC+AGG - chr5.4:31064004-31064023 MS.gene53071:intron 50.0%
! TCACGAGAAGGGACCTTTTG+TGG - chr5.4:31063950-31063969 MS.gene53071:intron 50.0%
! TCATGTTTGGCTCCATGCCT+CGG - chr5.4:31064080-31064099 MS.gene53071:intron 50.0%
!! CTGCAGATTCTGCCGCTTTA+TGG - chr5.4:31063771-31063790 MS.gene53071:intron 50.0%
!!! CCGCAAACTCGTGCCATTTT+AGG + chr5.4:31065576-31065595 None:intergenic 50.0%
AGAACTAAGGCCGCCTCTTC+TGG + chr5.4:31064844-31064863 None:intergenic 55.0%
TGGAGATGCCTCCTGTTAGC+TGG - chr5.4:31062716-31062735 MS.gene53071:intron 55.0%
!! AGCTAACAGGAGGCATCTCC+AGG + chr5.4:31062717-31062736 None:intergenic 55.0%
!! GCTAACAGGAGGCATCTCCA+GGG + chr5.4:31062716-31062735 None:intergenic 55.0%
CTCATCGCAGATGGCTGCCT+TGG - chr5.4:31065222-31065241 MS.gene53071:intron 60.0%
! GCCGCCTCTTCTGGTGTCAA+AGG + chr5.4:31064835-31064854 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 31061283 31065765 31061283 ID=MS.gene53071
chr5.4 mRNA 31061283 31065765 31061283 ID=MS.gene53071.t1;Parent=MS.gene53071
chr5.4 exon 31065537 31065765 31065537 ID=MS.gene53071.t1.exon1;Parent=MS.gene53071.t1
chr5.4 CDS 31065537 31065765 31065537 ID=cds.MS.gene53071.t1;Parent=MS.gene53071.t1
chr5.4 exon 31064705 31064772 31064705 ID=MS.gene53071.t1.exon2;Parent=MS.gene53071.t1
chr5.4 CDS 31064705 31064772 31064705 ID=cds.MS.gene53071.t1;Parent=MS.gene53071.t1
chr5.4 exon 31064535 31064623 31064535 ID=MS.gene53071.t1.exon3;Parent=MS.gene53071.t1
chr5.4 CDS 31064535 31064623 31064535 ID=cds.MS.gene53071.t1;Parent=MS.gene53071.t1
chr5.4 exon 31064283 31064445 31064283 ID=MS.gene53071.t1.exon4;Parent=MS.gene53071.t1
chr5.4 CDS 31064283 31064445 31064283 ID=cds.MS.gene53071.t1;Parent=MS.gene53071.t1
chr5.4 exon 31062982 31063271 31062982 ID=MS.gene53071.t1.exon5;Parent=MS.gene53071.t1
chr5.4 CDS 31062982 31063271 31062982 ID=cds.MS.gene53071.t1;Parent=MS.gene53071.t1
chr5.4 exon 31062079 31062283 31062079 ID=MS.gene53071.t1.exon6;Parent=MS.gene53071.t1
chr5.4 CDS 31062079 31062283 31062079 ID=cds.MS.gene53071.t1;Parent=MS.gene53071.t1
chr5.4 exon 31061722 31061879 31061722 ID=MS.gene53071.t1.exon7;Parent=MS.gene53071.t1
chr5.4 CDS 31061722 31061879 31061722 ID=cds.MS.gene53071.t1;Parent=MS.gene53071.t1
chr5.4 exon 31061283 31061553 31061283 ID=MS.gene53071.t1.exon8;Parent=MS.gene53071.t1
chr5.4 CDS 31061283 31061553 31061283 ID=cds.MS.gene53071.t1;Parent=MS.gene53071.t1
Gene Sequence

>MS.gene53071

ATGGAGGTTAGATCAGAGGGTATACGTTTGAGGTTTTGGTTTGTTCGTTTGTGTTCAAGTATTGTTCTTTGGATTTGTTTGGTTCAGTTAGTGACAGTGAGTGAACTATGGCATTCACATTTCATTTCTGGGATTACTACTGGTATATATAACATTGCTCAAATTCAACTTCCTAAACAAAATAATGTCGTGGTTGATCAACCGCCTCCGATTATTTTTCTTCCTCCAAGTTAGTTTCTTGACTCTAATTCTAGTCCTTTTTGTATCAGATATGTTTTTTGTTTTAATTTGATGCTTTAATGCAACCTTTGATATAAATTAATTGATCAATGAAGCTTTTGTGTATTGTTGATTAGGTGTTATGTGACTTATATTAGGTGTTAGATGGTTATAATTGTGATTAACTGAATTCAAGTGTAAATTGTATTTGTGTTGGTAAATTTTGTTTTTTTGGTTATGTGCTTGTTTTAATGTTGAATTGTGGATTGTGAAATCTGTTGGGAGCATGAATGATGTATGATGATTGTTAAGTGTTGTTCTATAGTGGTTTTTGAAATTGTCCTGGGGTTATTATAATTTGAAATGCTGATTCTAGGGTGTTACATTTATAGATAACTGTCGTATTATAATTAAATAATAACGGATATGATTATAAATAAAATTTAGTGAAATAAGTCACTATCGTTTATTTATTTTTAGATGATAAATCAATGTTCAATAAAAGGGATCTCCGGATATGCCCTTTTTAATAAGGTACATCTCAGGATATATGACGAGAACATTAGAAGTTACAAATTGAGAGAAAATCAGATCTTAATCTTAGTTGATACTAAGTGTTGCCATTAATTTTGAGGATAGGTATAGGATGTAAAGCAATATTGCTTATCGATGAATGGTTCTGATGGATAGGTATAGAATATTTTTGATTGTTTTACTTTTATTTTCTCACTCATTTTGGTAAATATTAAAGCTCTTTGACCTGACAACCTACAGGAAATTATACAAGTAATGGTTTTCTAAGAGTTTCCTGCAATGGTGGCTTGAATCAAATGCGTGCTGCGGTTTGTACTTCCTATTCTAAAATTTATCTGTTTGTATGATATCATAAGCTTAAATACTCATTTAGTGGCATGGTATTTCAGATATGTGACATGGTGACAATTGCTCGATTTTTGAATCTCACATTGATTGTGCCAGAGCTTGATAAGACATCATTTTGGGCAGACCCTAGGTATATACTTTGTTCTGATTTTTATTGTTTGAGTATCCTCTAATTTAACCTCCATATCATTCTAAAGTTGTGTAGTTTTCTTTTTGCAGTAATTTTGAGGACATTTTTGATGTGAAGCATTTCATTGATTCTTTACGAGACGAAGTTCGAATAGTGAAAAGAGTTCCTAAAAAGTTTAGCAGTAAATATGGATATTCTACCCTGGAGATGCCTCCTGTTAGCTGGTCCAATGAAAGATATTACTTGGAGCAGGTGTGTTTCTTTTATGAGCTGCGTTTGAGAAAACAGCTGAATAGTATTGTTCTAACTATTTGCATTTTAAGGTTTGATAATATATGATTGATTAATGATTCTTGACCTATATTTTTGGATAGTATGGCTTTCCCCAAACTGTTAATTTTTCAATGCTTTTCAGCACTGTTGTCAAATAGCAGCGGTAGCGCTATATCATAGCAGATTTTGATGAATCTTCTATGAAAACTTCGAAGCTATCCTAGTTTCGTTATTAGGGCAGTAGCGACCGCTATTCCAGTTTCTGCTAGTTGATTCAATGGCAATAGTGGAATAAAATAGAATTATAGGGCTATTTTTTGAAGAATAAAAATGAAGAATCTGCGCGTGTGCCAAACATGACCTATTGTCTTATTAGAAAACTGAAAGATTCAGTGTGTATTACTTTTCAATATTAAGCTATTTCTACCTTTTAAAGAACATGACCTATTCACCATATATGTAAGCCTTTCAAAATATAAGCCATGTTCTGCTTTATTCCCTTTTTATTTGTCTGCAACATGCCTTTTTTTTTTTTTTTTTTCCCTTTCAAACATATAATTGACTTATTTCAACTGCTATGCGGCTTCCACTATTTGCCCACAATGCTATCTTGTATTTCACATAGCGGATTTTTGGCGTTCCTTTATTTTCTGCAATCCACTATTGATAACACTGATTTTCAAAGAAGTTATGGGAGCTTTTGTGTCCTTTCCAACTTGATTGGAACTTTAGAATTATCGATAATTATGAATCAGTCACATGAAGAAATTTATAGTTATGGGAAGAAATTTATAGTTGTTTTCCTATTTAGTAAACTTGGTTTTTAGCAACTAAAATAAGTTGCTTTTGGTGTAGGATAGCCAACATAGGTTCCAAAAAATAGCACTTCGCTGAGTGTATAAAAGTATGATAAGATATAACGATTTTTTCTCTAACTTTGAGAAGTATATACATTTTGTTTAATCCAACACTATTGACTCTACACTGCAGATTCTGCCGCTTTATGGAAAGCACAAGGTGTTACACTTCAACAGAACTGACGCACGTCTAGCAAATAACGGTCTCCCACTTGATCTACAGAAACTCAGGTGTCGTGTCAATTACGAGGCTATTAAATTCACTCCTCAAATTGAGAATTTAGGGCGAAAATTGATTCGGATGCTTCACGAGAAGGGACCTTTTGTGGCACTGCATCTTAGATATGAGATGGACATGTTGGCTTTCTCAGGTTGTACTCAGGGTTGCTCAGAGAAAGAAGCCGAGGAACTCAAAAGATTGAGGTATTTTTATGTTTCATGTTTGGCTCCATGCCTCGGCTTCTAATTCATTCTTGCTTCTGTAATTGAGGATAAGAGGGATTTGGATCTTCTCGGTTTCTGAATAACAGGATATGTTCCTCATATTAGTAAATGATATTTACTTGTGCTCTGATAGAGAAACCTTTTGGATACGAGAGTAGTGGTTATGTATCTCATGAGTTATTAAATTATTTAGAGATATTAAGGCTCTTTTACATTTAGAAAATCTCTTCTGTTTTTATTTTCCTTCTACGGTTTTTCGTTACAAAACTGTCATATTTTTTCAATCCATTTCTTGTTTTCATAGACTTTGTACATGCAACACTGACAACAATTTTTTTATTTATTTTTAGATATTAAAGCCCTTTTACTTTTGGGAAACGTTTTCTGTTTGTGCTTTTTAATTACAAAGCTGTCAGATTGTTTTCAATCCATTTATTGTGTTTTTAGTATTATCTCTTGTACACGTGGTGGATTTGTAATTCAAAAAGGAAAACATGAAATGAAATCAGAAACATTTTCTTAAATTGAACAGACCCTTATTTACTTGATTATATGCTAACAAATATTATTGTGATTTAAGATTCTGTGGCACCCTTACAGCAATTAGATATCTATATGACATCAAATGTTGATTACTTGATTATATGCTAATAAAACTGTCCCTTTTTGTAATTCAGGTATGCATTCCCTTGGTGGAGAGAAAAGGAGATAATTTCTGAAGAGAGGAGATCACAGGGTCTATGTCCTTTGACACCAGAAGAGGCGGCCTTAGTTCTTCGAGCCTTAGGTTTTGGTAGGGAGACACCGATATATATTGCAGCTGGTGAGATTTATGGCGGTGAACGTAGGCTTGCACAACTCAGAGCAGCATTTCCCCAACTTGTGAGTTTGTCAACATTTTAACTTCCTTCCATTTTTCTGCTCATTGTTTGAGACATCACACAACTGTTGAAAATTGTTCTTGCGCTCATTAATGGTTGGACCTGATCTGAAAGATGCATTTTCTCTCAATGATCCTCATTTTCAGTCTTTGAAATTATTCTGCTGTGCTACATCTGACTTTCTGTGTTTGCAATTTCAGGTGAAAAAGGAAATGTTGCTGACACGTGATGATTTGCAGCAGTTCCAGAATCACTCATCGCAGATGGCTGCCTTGGATTTTATGGTTTCAGTAGCCAGTGACACCTTTATCCCTACTTATGATGGGAACATGGCAAAACTTGTGGAAGGCCATCGAAGGTATGTATTTACCCGAATGTTGCTGACATGTTATTAATACATTATTGTTGATACATTATCTATTATTGTCAATACATTAGAGGGACCAAAGTGAAATTTGTGTAATAACAGTAAGTGCATTATTGGTATTATTCCTCTTTAATTTCTAACTAATTTGTTCCTCTGCAGGTATTCCGATTTTAGAAAAACAATCTTGTTGGATCGAAAAAAGGTCGTGGAATTGGTTGATATGCATCATAATGGAACCCTAAAATGGCACGAGTTTGCGGATGGCGTAAAACAAGTTCATGAAAAGAGAATTGGACAGCCAACTCGTCGAAAAGTTATAATCGACAGACCAAAGGAGGAGGACTATTTCTATGCAAACCCTCATGAGTGTCTATGTGAGGAAAGAAATTGTGATGAGTTTCTAGGTCATCACAACTCTAGTCAAGTATAG
Protein sequence

>MS.gene53071.t1

MEVRSEGIRLRFWFVRLCSSIVLWICLVQLVTVSELWHSHFISGITTGIYNIAQIQLPKQNNVVVDQPPPIIFLPPRNYTSNGFLRVSCNGGLNQMRAAICDMVTIARFLNLTLIVPELDKTSFWADPSNFEDIFDVKHFIDSLRDEVRIVKRVPKKFSSKYGYSTLEMPPVSWSNERYYLEQILPLYGKHKVLHFNRTDARLANNGLPLDLQKLRCRVNYEAIKFTPQIENLGRKLIRMLHEKGPFVALHLRYEMDMLAFSGCTQGCSEKEAEELKRLRYAFPWWREKEIISEERRSQGLCPLTPEEAALVLRALGFGRETPIYIAAGEIYGGERRLAQLRAAFPQLVKKEMLLTRDDLQQFQNHSSQMAALDFMVSVASDTFIPTYDGNMAKLVEGHRRYSDFRKTILLDRKKVVELVDMHHNGTLKWHEFADGVKQVHEKRIGQPTRRKVIIDRPKEEDYFYANPHECLCEERNCDEFLGHHNSSQV