Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007066.t1 | XP_013441621.1 | 100 | 112 | 0 | 0 | 1 | 112 | 1 | 112 | 1.10E-57 | 232.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007066.t1 | Q9LMK0 | 91.1 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 9.1e-56 | 217.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007066.t1 | I3T9M0 | 100.0 | 112 | 0 | 0 | 1 | 112 | 1 | 112 | 8.2e-58 | 232.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene018553 | MS.gene007066 | PPI |
| MS.gene007066 | MS.gene39294 | PPI |
| MS.gene007066 | MS.gene051903 | PPI |
| MS.gene007066 | MS.gene23059 | PPI |
| MS.gene23058 | MS.gene007066 | PPI |
| MS.gene29581 | MS.gene007066 | PPI |
| MS.gene007066 | MS.gene007067 | PPI |
| MS.gene007066 | MS.gene022752 | PPI |
| MS.gene061905 | MS.gene007066 | PPI |
| MS.gene56696 | MS.gene007066 | PPI |
| MS.gene007066 | MS.gene070073 | PPI |
| MS.gene007066 | MS.gene64512 | PPI |
| MS.gene007066 | MS.gene020215 | PPI |
| MS.gene09845 | MS.gene007066 | PPI |
| MS.gene45646 | MS.gene007066 | PPI |
| MS.gene051903 | MS.gene007066 | PPI |
| MS.gene051904 | MS.gene007066 | PPI |
| MS.gene007066 | MS.gene80639 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007066.t1 | MTR_0699s0020 | 100.000 | 112 | 0 | 0 | 1 | 112 | 1 | 112 | 2.57e-79 | 228 |
| MS.gene007066.t1 | MTR_0699s0010 | 99.107 | 112 | 1 | 0 | 1 | 112 | 1 | 112 | 1.25e-78 | 226 |
| MS.gene007066.t1 | MTR_5g081705 | 94.643 | 112 | 6 | 0 | 1 | 112 | 26 | 137 | 5.74e-77 | 223 |
| MS.gene007066.t1 | MTR_5g081705 | 94.949 | 99 | 5 | 0 | 1 | 99 | 26 | 124 | 2.98e-67 | 198 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene007066.t1 | AT1G74270 | 91.964 | 112 | 9 | 0 | 1 | 112 | 1 | 112 | 6.63e-74 | 214 |
| MS.gene007066.t1 | AT1G07070 | 91.071 | 112 | 10 | 0 | 1 | 112 | 1 | 112 | 7.40e-74 | 214 |
| MS.gene007066.t1 | AT3G55750 | 87.387 | 111 | 14 | 0 | 2 | 112 | 1 | 111 | 4.97e-71 | 207 |
| MS.gene007066.t1 | AT1G41880 | 86.486 | 111 | 15 | 0 | 2 | 112 | 1 | 111 | 7.54e-71 | 207 |
| MS.gene007066.t1 | AT1G41880 | 86.486 | 111 | 15 | 0 | 2 | 112 | 1 | 111 | 7.54e-71 | 207 |
Find 48 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGAAGATTTGACTTGAATT+TGG | 0.232635 | 7.3:+6844362 | None:intergenic |
| TTAAATTATTACCATTGATT+TGG | 0.240677 | 7.3:+6844338 | None:intergenic |
| TGGTGTTCACTCCTTCAATT+TGG | 0.242520 | 7.3:+6844520 | None:intergenic |
| TAAATTATTACCATTGATTT+GGG | 0.268294 | 7.3:+6844339 | None:intergenic |
| GCCCCATATGCAGCGGTAAT+GGG | 0.380708 | 7.3:+6844425 | None:intergenic |
| CCAGAGATGTGTTTGGGTAC+TGG | 0.398125 | 7.3:+6844544 | None:intergenic |
| ATTTGGACCAGAGATGTGTT+TGG | 0.405595 | 7.3:+6844537 | None:intergenic |
| GAACCCATTACCGCTGCATA+TGG | 0.409627 | 7.3:-6844428 | MS.gene007066:CDS |
| TGCCCCATATGCAGCGGTAA+TGG | 0.410420 | 7.3:+6844424 | None:intergenic |
| GACGTCAAGGAGAGCGTGTC+AGG | 0.412439 | 7.3:-6845204 | MS.gene007066:intron |
| TTTGGACCAGAGATGTGTTT+GGG | 0.422500 | 7.3:+6844538 | None:intergenic |
| TTGGTATGCTGGCAAGAAAA+TGG | 0.428506 | 7.3:-6844484 | MS.gene007066:CDS |
| AGTTTACCTCATATATTGCT+TGG | 0.429962 | 7.3:+6844215 | None:intergenic |
| GAAGAGGTAGCTTGGTATGC+TGG | 0.459649 | 7.3:-6844495 | MS.gene007066:CDS |
| AACCCATTACCGCTGCATAT+GGG | 0.459805 | 7.3:-6844427 | MS.gene007066:CDS |
| ACATCTCTGGTCCAAATTGA+AGG | 0.462436 | 7.3:-6844531 | MS.gene007066:CDS |
| GTTTACCTCATATATTGCTT+GGG | 0.467769 | 7.3:+6844216 | None:intergenic |
| TACGTGAGGGGTACAATCCT+TGG | 0.470557 | 7.3:-6844661 | MS.gene007066:CDS |
| GGTTCCATTCTTCTTCACCT+TGG | 0.492441 | 7.3:+6844446 | None:intergenic |
| GTACAATCCTTGGATACAAA+AGG | 0.498010 | 7.3:-6844651 | MS.gene007066:intron |
| AAGGCCAAGGTGAAGAAGAA+TGG | 0.517743 | 7.3:-6844450 | MS.gene007066:CDS |
| GTTTGGGTACTGGTTTACTT+TGG | 0.518411 | 7.3:+6844554 | None:intergenic |
| ATACCAAGCTACCTCTTCCT+TGG | 0.523366 | 7.3:+6844500 | None:intergenic |
| GCATATGGGGCAAGGTTACA+AGG | 0.526923 | 7.3:-6844413 | MS.gene007066:CDS |
| CCAGTACCCAAACACATCTC+TGG | 0.530530 | 7.3:-6844544 | MS.gene007066:CDS |
| TTGTATTAGGCTTTACGTGA+GGG | 0.533599 | 7.3:-6844674 | MS.gene007066:intron |
| ACCCATTACCGCTGCATATG+GGG | 0.539984 | 7.3:-6844426 | MS.gene007066:CDS |
| TTTGTATTAGGCTTTACGTG+AGG | 0.553810 | 7.3:-6844675 | MS.gene007066:intron |
| AGGAGTGAACACCAAGGAAG+AGG | 0.555068 | 7.3:-6844511 | MS.gene007066:CDS |
| ATGTACCCAAGCAATATATG+AGG | 0.556382 | 7.3:-6844221 | None:intergenic |
| AATTATTACCATTGATTTGG+GGG | 0.558553 | 7.3:+6844341 | None:intergenic |
| GGCATATATCTACAAGGCCA+AGG | 0.574061 | 7.3:-6844463 | MS.gene007066:CDS |
| AAATTATTACCATTGATTTG+GGG | 0.588097 | 7.3:+6844340 | None:intergenic |
| ACACCAAGGAAGAGGTAGCT+TGG | 0.593143 | 7.3:-6844503 | MS.gene007066:CDS |
| GTACATAAATACTCTTACGC+GGG | 0.601920 | 7.3:+6844238 | None:intergenic |
| TGTATTAGGCTTTACGTGAG+GGG | 0.604275 | 7.3:-6844673 | MS.gene007066:intron |
| GGCAAGGTTACAAGGCCACA+TGG | 0.606069 | 7.3:-6844405 | MS.gene007066:CDS |
| AAGATGGTGAAGGGACGTCA+AGG | 0.609822 | 7.3:-6845217 | MS.gene007066:CDS |
| ATTATTACCATTGATTTGGG+GGG | 0.627666 | 7.3:+6844342 | None:intergenic |
| GAAAATGGCATATATCTACA+AGG | 0.630318 | 7.3:-6844469 | MS.gene007066:CDS |
| GGTACATAAATACTCTTACG+CGG | 0.638869 | 7.3:+6844237 | None:intergenic |
| TTACCGCTGCATATGGGGCA+AGG | 0.645005 | 7.3:-6844421 | MS.gene007066:CDS |
| AGTTGCCGTGAAGATGGTGA+AGG | 0.661868 | 7.3:-6845227 | None:intergenic |
| TAACCTTGCCCCATATGCAG+CGG | 0.677626 | 7.3:+6844418 | None:intergenic |
| ACAAGGCCACATGGTAACAG+TGG | 0.690947 | 7.3:-6844396 | MS.gene007066:CDS |
| GTTGCCGTGAAGATGGTGAA+GGG | 0.718264 | 7.3:-6845226 | None:intergenic |
| ACAATACCACTGTTACCATG+TGG | 0.724821 | 7.3:+6844390 | None:intergenic |
| AATTGAAGGAGTGAACACCA+AGG | 0.776962 | 7.3:-6844517 | MS.gene007066:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TATAATATTAGCTTATTATA+TGG | + | chr7.3:6844731-6844750 | None:intergenic | 10.0% |
| !!! | TTTTTTTTAAATCATCATTT+GGG | - | chr7.3:6845130-6845149 | MS.gene007066:intron | 10.0% |
| !!! | TTTTTTTTTAAATCATCATT+TGG | - | chr7.3:6845129-6845148 | MS.gene007066:intron | 10.0% |
| !!! | AAACATGAAAATTTTCAAAT+TGG | + | chr7.3:6844628-6844647 | None:intergenic | 15.0% |
| !!! | ATCATTGTTTTTAATTCATT+TGG | - | chr7.3:6844385-6844404 | MS.gene007066:CDS | 15.0% |
| !!! | ATTTATTTTTGAATGATAGT+AGG | + | chr7.3:6844447-6844466 | None:intergenic | 15.0% |
| !!! | ATTTTTGTTTCATTTGTATT+AGG | - | chr7.3:6844750-6844769 | MS.gene007066:intron | 15.0% |
| !!! | TAAATTATTACCATTGATTT+GGG | + | chr7.3:6845101-6845120 | None:intergenic | 15.0% |
| !!! | TTAAATTATTACCATTGATT+TGG | + | chr7.3:6845102-6845121 | None:intergenic | 15.0% |
| !!! | AAATTATTACCATTGATTTG+GGG | + | chr7.3:6845100-6845119 | None:intergenic | 20.0% |
| ! | ATCTGAAAATCAACTGTATT+TGG | + | chr7.3:6844576-6844595 | None:intergenic | 25.0% |
| ! | TAGTTTACAGAGTAAAGTAA+AGG | - | chr7.3:6844501-6844520 | MS.gene007066:CDS | 25.0% |
| !! | ACTTTTCTAATGAAAATGTC+TGG | - | chr7.3:6844812-6844831 | MS.gene007066:intron | 25.0% |
| !!! | AATTATTACCATTGATTTGG+GGG | + | chr7.3:6845099-6845118 | None:intergenic | 25.0% |
| GAAAATCAACTGTATTTGGT+TGG | + | chr7.3:6844572-6844591 | None:intergenic | 30.0% | |
| GAAAATGGCATATATCTACA+AGG | - | chr7.3:6844968-6844987 | MS.gene007066:intron | 30.0% | |
| TCGTGTTTGGATTAATTTCA+GGG | - | chr7.3:6845174-6845193 | MS.gene007066:intron | 30.0% | |
| TGTTATCTTAATCTCGTGTT+TGG | - | chr7.3:6845161-6845180 | MS.gene007066:intron | 30.0% | |
| ! | AAGTTTACCTTTTGTATCCA+AGG | + | chr7.3:6844796-6844815 | None:intergenic | 30.0% |
| ! | TAAATACGCATAGTTAGTCT+AGG | + | chr7.3:6844692-6844711 | None:intergenic | 30.0% |
| !! | ATTATTACCATTGATTTGGG+GGG | + | chr7.3:6845098-6845117 | None:intergenic | 30.0% |
| AAATGTCTGGTTAGATGCTT+TGG | - | chr7.3:6844825-6844844 | MS.gene007066:intron | 35.0% | |
| AGAGATGAAACTACAGAATC+AGG | + | chr7.3:6844294-6844313 | None:intergenic | 35.0% | |
| CTCGTGTTTGGATTAATTTC+AGG | - | chr7.3:6845173-6845192 | MS.gene007066:intron | 35.0% | |
| GGTACATAAATACTCTTACG+CGG | + | chr7.3:6845203-6845222 | None:intergenic | 35.0% | |
| GTACAATCCTTGGATACAAA+AGG | - | chr7.3:6844786-6844805 | MS.gene007066:intron | 35.0% | |
| GTACATAAATACTCTTACGC+GGG | + | chr7.3:6845202-6845221 | None:intergenic | 35.0% | |
| TTGTATTAGGCTTTACGTGA+GGG | - | chr7.3:6844763-6844782 | MS.gene007066:intron | 35.0% | |
| TTTGTATTAGGCTTTACGTG+AGG | - | chr7.3:6844762-6844781 | MS.gene007066:intron | 35.0% | |
| !! | GGGAAGATTTGACTTGAATT+TGG | + | chr7.3:6845078-6845097 | None:intergenic | 35.0% |
| AAATCTTCCCCCCAAATCAA+TGG | - | chr7.3:6845088-6845107 | MS.gene007066:intron | 40.0% | |
| AATTGAAGGAGTGAACACCA+AGG | - | chr7.3:6844920-6844939 | MS.gene007066:intron | 40.0% | |
| ACAATACCACTGTTACCATG+TGG | + | chr7.3:6845050-6845069 | None:intergenic | 40.0% | |
| ACATCTCTGGTCCAAATTGA+AGG | - | chr7.3:6844906-6844925 | MS.gene007066:intron | 40.0% | |
| ATTTGGACCAGAGATGTGTT+TGG | + | chr7.3:6844903-6844922 | None:intergenic | 40.0% | |
| TGGTGTTCACTCCTTCAATT+TGG | + | chr7.3:6844920-6844939 | None:intergenic | 40.0% | |
| TGTATTAGGCTTTACGTGAG+GGG | - | chr7.3:6844764-6844783 | MS.gene007066:intron | 40.0% | |
| TTGGTATGCTGGCAAGAAAA+TGG | - | chr7.3:6844953-6844972 | MS.gene007066:intron | 40.0% | |
| TTTGGACCAGAGATGTGTTT+GGG | + | chr7.3:6844902-6844921 | None:intergenic | 40.0% | |
| !! | GTTTGGGTACTGGTTTACTT+TGG | + | chr7.3:6844886-6844905 | None:intergenic | 40.0% |
| AACCCATTACCGCTGCATAT+GGG | - | chr7.3:6845010-6845029 | MS.gene007066:intron | 45.0% | |
| AAGGCCAAGGTGAAGAAGAA+TGG | - | chr7.3:6844987-6845006 | MS.gene007066:intron | 45.0% | |
| ATACCAAGCTACCTCTTCCT+TGG | + | chr7.3:6844940-6844959 | None:intergenic | 45.0% | |
| GGCATATATCTACAAGGCCA+AGG | - | chr7.3:6844974-6844993 | MS.gene007066:intron | 45.0% | |
| GGTTCCATTCTTCTTCACCT+TGG | + | chr7.3:6844994-6845013 | None:intergenic | 45.0% | |
| ACAAGGCCACATGGTAACAG+TGG | - | chr7.3:6845041-6845060 | MS.gene007066:intron | 50.0% | |
| ACACCAAGGAAGAGGTAGCT+TGG | - | chr7.3:6844934-6844953 | MS.gene007066:intron | 50.0% | |
| ACCCATTACCGCTGCATATG+GGG | - | chr7.3:6845011-6845030 | MS.gene007066:intron | 50.0% | |
| AGGAGTGAACACCAAGGAAG+AGG | - | chr7.3:6844926-6844945 | MS.gene007066:intron | 50.0% | |
| CCAGAGATGTGTTTGGGTAC+TGG | + | chr7.3:6844896-6844915 | None:intergenic | 50.0% | |
| CCAGTACCCAAACACATCTC+TGG | - | chr7.3:6844893-6844912 | MS.gene007066:intron | 50.0% | |
| GAACCCATTACCGCTGCATA+TGG | - | chr7.3:6845009-6845028 | MS.gene007066:intron | 50.0% | |
| GAAGAGGTAGCTTGGTATGC+TGG | - | chr7.3:6844942-6844961 | MS.gene007066:intron | 50.0% | |
| GCATATGGGGCAAGGTTACA+AGG | - | chr7.3:6845024-6845043 | MS.gene007066:intron | 50.0% | |
| TAACCTTGCCCCATATGCAG+CGG | + | chr7.3:6845022-6845041 | None:intergenic | 50.0% | |
| ! | TACGTGAGGGGTACAATCCT+TGG | - | chr7.3:6844776-6844795 | MS.gene007066:intron | 50.0% |
| GCCCCATATGCAGCGGTAAT+GGG | + | chr7.3:6845015-6845034 | None:intergenic | 55.0% | |
| GGCAAGGTTACAAGGCCACA+TGG | - | chr7.3:6845032-6845051 | MS.gene007066:intron | 55.0% | |
| TGCCCCATATGCAGCGGTAA+TGG | + | chr7.3:6845016-6845035 | None:intergenic | 55.0% | |
| TTACCGCTGCATATGGGGCA+AGG | - | chr7.3:6845016-6845035 | MS.gene007066:intron | 55.0% | |
| ! | GACGTCAAGGAGAGCGTGTC+AGG | - | chr7.3:6844233-6844252 | MS.gene007066:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 6844223 | 6845236 | 6844223 | ID=MS.gene007066 |
| chr7.3 | mRNA | 6844223 | 6845236 | 6844223 | ID=MS.gene007066.t1;Parent=MS.gene007066 |
| chr7.3 | exon | 6845205 | 6845236 | 6845205 | ID=MS.gene007066.t1.exon1;Parent=MS.gene007066.t1 |
| chr7.3 | CDS | 6845205 | 6845236 | 6845205 | ID=cds.MS.gene007066.t1;Parent=MS.gene007066.t1 |
| chr7.3 | exon | 6844652 | 6844687 | 6844652 | ID=MS.gene007066.t1.exon2;Parent=MS.gene007066.t1 |
| chr7.3 | CDS | 6844652 | 6844687 | 6844652 | ID=cds.MS.gene007066.t1;Parent=MS.gene007066.t1 |
| chr7.3 | exon | 6844350 | 6844578 | 6844350 | ID=MS.gene007066.t1.exon3;Parent=MS.gene007066.t1 |
| chr7.3 | CDS | 6844350 | 6844578 | 6844350 | ID=cds.MS.gene007066.t1;Parent=MS.gene007066.t1 |
| chr7.3 | exon | 6844223 | 6844264 | 6844223 | ID=MS.gene007066.t1.exon4;Parent=MS.gene007066.t1 |
| chr7.3 | CDS | 6844223 | 6844264 | 6844223 | ID=cds.MS.gene007066.t1;Parent=MS.gene007066.t1 |
| Gene Sequence |
| Protein sequence |
