AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23059


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23059.t1 MTR_0699s0020 100.000 112 0 0 1 112 1 112 2.57e-79 228
MS.gene23059.t1 MTR_0699s0010 99.107 112 1 0 1 112 1 112 1.25e-78 226
MS.gene23059.t1 MTR_5g081705 94.643 112 6 0 1 112 26 137 5.74e-77 223
MS.gene23059.t1 MTR_5g081705 94.949 99 5 0 1 99 26 124 2.98e-67 198
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23059.t1 AT1G74270 91.964 112 9 0 1 112 1 112 6.63e-74 214
MS.gene23059.t1 AT1G07070 91.071 112 10 0 1 112 1 112 7.40e-74 214
MS.gene23059.t1 AT3G55750 87.387 111 14 0 2 112 1 111 4.97e-71 207
MS.gene23059.t1 AT1G41880 86.486 111 15 0 2 112 1 111 7.54e-71 207
MS.gene23059.t1 AT1G41880 86.486 111 15 0 2 112 1 111 7.54e-71 207

Find 45 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGAAGATTTGATTTGAATT+TGG 0.236788 7.2:+7229866 None:intergenic
TGGTGTTCACTCCTTCAATT+TGG 0.242520 7.2:+7230024 None:intergenic
GTAAATTATTACCATTGATT+TGG 0.266957 7.2:+7229842 None:intergenic
TAAATTATTACCATTGATTT+GGG 0.343670 7.2:+7229843 None:intergenic
GCCCCATATGCAACGGTAAT+GGG 0.353096 7.2:+7229929 None:intergenic
GAACCCATTACCGTTGCATA+TGG 0.373177 7.2:-7229932 MS.gene23059:CDS
GGTTTACCTCATATGTTACT+TGG 0.390121 7.2:+7229720 None:intergenic
CCAGAGATGTGTTTGGGTAC+TGG 0.398125 7.2:+7230048 None:intergenic
TTGGTATGCCGGCAAGAAAA+TGG 0.403183 7.2:-7229988 MS.gene23059:CDS
ATTTGGACCAGAGATGTGTT+TGG 0.405595 7.2:+7230041 None:intergenic
GACGTCAAGGAGAGCGTGTC+AGG 0.412439 7.2:-7231179 MS.gene23059:intron
TTTGGACCAGAGATGTGTTT+GGG 0.422500 7.2:+7230042 None:intergenic
TGCCCCATATGCAACGGTAA+TGG 0.448613 7.2:+7229928 None:intergenic
AACCCATTACCGTTGCATAT+GGG 0.460048 7.2:-7229931 MS.gene23059:CDS
ACATCTCTGGTCCAAATTGA+AGG 0.462436 7.2:-7230035 MS.gene23059:CDS
TACGTGAGGGGTACAATCCT+TGG 0.470557 7.2:-7230198 MS.gene23059:CDS
GTACAATCCTTGGATACAAA+AGG 0.498010 7.2:-7230188 MS.gene23059:intron
ATACCAAGCTACCTCTTCCT+TGG 0.508204 7.2:+7230004 None:intergenic
GAAGAGGTAGCTTGGTATGC+CGG 0.510952 7.2:-7229999 MS.gene23059:CDS
GGTTCCGTTCTTCTTCACCT+TGG 0.512346 7.2:+7229950 None:intergenic
GTTTACCTCATATGTTACTT+GGG 0.516767 7.2:+7229721 None:intergenic
AAATCTTCCTCCCAAATCAA+TGG 0.516979 7.2:-7229853 MS.gene23059:intron
GTTTGGGTACTGGTTTACTT+TGG 0.518411 7.2:+7230058 None:intergenic
ACCCATTACCGTTGCATATG+GGG 0.524799 7.2:-7229930 MS.gene23059:CDS
GCATATGGGGCAAGGTTACA+AGG 0.526923 7.2:-7229917 MS.gene23059:CDS
CCAGTACCCAAACACATCTC+TGG 0.530530 7.2:-7230048 MS.gene23059:CDS
TTGTATTAGGCTTTACGTGA+GGG 0.535618 7.2:-7230211 MS.gene23059:intron
AGGAGTGAACACCAAGGAAG+AGG 0.555068 7.2:-7230015 MS.gene23059:CDS
ATGTACCCAAGTAACATATG+AGG 0.563414 7.2:-7229726 None:intergenic
GGCATATATCTACAAGGCCA+AGG 0.574061 7.2:-7229967 MS.gene23059:CDS
GGCAAGGTTACAAGGCCTCA+TGG 0.577972 7.2:-7229909 MS.gene23059:CDS
TTTGTATTAGGCTTTACGTG+AGG 0.589429 7.2:-7230212 MS.gene23059:intron
TTACCGTTGCATATGGGGCA+AGG 0.593071 7.2:-7229925 MS.gene23059:CDS
ACACCAAGGAAGAGGTAGCT+TGG 0.593143 7.2:-7230007 MS.gene23059:CDS
TGTATTAGGCTTTACGTGAG+GGG 0.606295 7.2:-7230210 MS.gene23059:intron
AAGATGGTGAAGGGACGTCA+AGG 0.609822 7.2:-7231192 MS.gene23059:CDS
TGCTGCTGTGAAGATGGTGA+AGG 0.610958 7.2:-7231202 None:intergenic
ATTATTACCATTGATTTGGG+AGG 0.614066 7.2:+7229846 None:intergenic
AAGGCCAAGGTGAAGAAGAA+CGG 0.630167 7.2:-7229954 MS.gene23059:CDS
GAAAATGGCATATATCTACA+AGG 0.630318 7.2:-7229973 MS.gene23059:CDS
GCTGCTGTGAAGATGGTGAA+GGG 0.632435 7.2:-7231201 None:intergenic
TAACCTTGCCCCATATGCAA+CGG 0.648972 7.2:+7229922 None:intergenic
ACAAGGCCTCATGGTAACAG+TGG 0.683556 7.2:-7229900 MS.gene23059:CDS
ACAATACCACTGTTACCATG+AGG 0.737809 7.2:+7229894 None:intergenic
AATTGAAGGAGTGAACACCA+AGG 0.776962 7.2:-7230021 MS.gene23059:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATGAAAATACAAATAAAAA+TGG + chr7.2:7229779-7229798 None:intergenic 15.0%
!! TAATAAGAATAAAAATGTGT+TGG + chr7.2:7231100-7231119 None:intergenic 15.0%
!! TATAGAAAAGGAAAAAATAA+AGG + chr7.2:7230238-7230257 None:intergenic 15.0%
!!! TAAAGCTATTTTTATAGAAA+AGG + chr7.2:7230250-7230269 None:intergenic 15.0%
!!! TAAATTATTACCATTGATTT+GGG + chr7.2:7231077-7231096 None:intergenic 15.0%
!!! TATCATTGTTTTAATTCATT+TGG - chr7.2:7229838-7229857 MS.gene23059:intron 15.0%
!!! TTTTGGTTTTATTTTTGTTA+GGG - chr7.2:7230479-7230498 MS.gene23059:intron 15.0%
!! AAAAGACACAAAAACATATT+TGG + chr7.2:7230592-7230611 None:intergenic 20.0%
!! AAATATAGCTGCTAAAAAAT+TGG + chr7.2:7230371-7230390 None:intergenic 20.0%
!! AATATAGCTGCTAAAAAATT+GGG + chr7.2:7230370-7230389 None:intergenic 20.0%
!! TTTCTTGTTCAATTTGTATT+AGG - chr7.2:7230693-7230712 MS.gene23059:intron 20.0%
!!! ATTTTTGTTAGGGATTAAAT+TGG - chr7.2:7230489-7230508 MS.gene23059:intron 20.0%
!!! CATCTGTTTTATTTTATGTT+TGG - chr7.2:7230768-7230787 MS.gene23059:intron 20.0%
!!! CTTTTGGTTTTATTTTTGTT+AGG - chr7.2:7230478-7230497 MS.gene23059:intron 20.0%
!!! GTAAATTATTACCATTGATT+TGG + chr7.2:7231078-7231097 None:intergenic 20.0%
!!! TTTTTGTTAGGGATTAAATT+GGG - chr7.2:7230490-7230509 MS.gene23059:intron 20.0%
! TAGTCCAGTTATAAAAATGT+CGG + chr7.2:7230427-7230446 None:intergenic 25.0%
!!! AGGAAGATTTGATTTGAATT+TGG + chr7.2:7231054-7231073 None:intergenic 25.0%
AAAAAAAGAGAGAGAGAAGT+AGG + chr7.2:7230140-7230159 None:intergenic 30.0%
AAAATATTGTGGGACGAAAA+TGG - chr7.2:7230517-7230536 MS.gene23059:intron 30.0%
AAATATTGTGGGACGAAAAT+GGG - chr7.2:7230518-7230537 MS.gene23059:intron 30.0%
ACAAAAATGAAATCTGAACC+TGG - chr7.2:7230398-7230417 MS.gene23059:intron 30.0%
ACATTAGCATGCAGTTAATA+AGG - chr7.2:7229885-7229904 MS.gene23059:CDS 30.0%
AGTCCAGTTATAAAAATGTC+GGG + chr7.2:7230426-7230445 None:intergenic 30.0%
AGTCTCACAAAAACTTATGT+TGG + chr7.2:7230033-7230052 None:intergenic 30.0%
CATTAGCATGCAGTTAATAA+GGG - chr7.2:7229886-7229905 MS.gene23059:CDS 30.0%
GAAAATGGCATATATCTACA+AGG - chr7.2:7230944-7230963 MS.gene23059:intron 30.0%
! AAGTTTACCTTTTGTATCCA+AGG + chr7.2:7230739-7230758 None:intergenic 30.0%
! ATCACTCCACAAAGTATTAA+GGG + chr7.2:7230331-7230350 None:intergenic 30.0%
! GTTTGGTTTATGAAAATGTC+TGG - chr7.2:7230785-7230804 MS.gene23059:intron 30.0%
!! ATTATTACCATTGATTTGGG+AGG + chr7.2:7231074-7231093 None:intergenic 30.0%
!! GTTCAGATTTCATTTTTGTC+AGG + chr7.2:7230397-7230416 None:intergenic 30.0%
!!! ATGCTATGAGCTGTTTTTAT+AGG + chr7.2:7230199-7230218 None:intergenic 30.0%
!!! TTATCTTGACTTTTTGAGTG+TGG - chr7.2:7231136-7231155 MS.gene23059:intron 30.0%
AAATCTTCCTCCCAAATCAA+TGG - chr7.2:7231064-7231083 MS.gene23059:intron 35.0%
AAATGTCTGGTTAGATGCTT+TGG - chr7.2:7230798-7230817 MS.gene23059:intron 35.0%
AACATGCCCTTAATACTTTG+TGG - chr7.2:7230322-7230341 MS.gene23059:intron 35.0%
AATTGGGTCGCAAAATATTG+TGG - chr7.2:7230506-7230525 MS.gene23059:intron 35.0%
ACTGCATGCTAATGTTTGAA+TGG + chr7.2:7229880-7229899 None:intergenic 35.0%
AGAACTTGTGAAAATCAGCT+GGG + chr7.2:7230108-7230127 None:intergenic 35.0%
ATAGCTGCTAAAAAATTGGG+AGG + chr7.2:7230367-7230386 None:intergenic 35.0%
ATTGGGTCGCAAAATATTGT+GGG - chr7.2:7230507-7230526 MS.gene23059:intron 35.0%
CTCACAAAAACTTATGTTGG+TGG + chr7.2:7230030-7230049 None:intergenic 35.0%
CTTAATACTTTGTGGAGTGA+TGG - chr7.2:7230330-7230349 MS.gene23059:intron 35.0%
GTACAATCCTTGGATACAAA+AGG - chr7.2:7230729-7230748 MS.gene23059:intron 35.0%
GTCCAGTTATAAAAATGTCG+GGG + chr7.2:7230425-7230444 None:intergenic 35.0%
TCAAATAAGCCAATCCAAAC+AGG + chr7.2:7230001-7230020 None:intergenic 35.0%
TCGCTAGTATGAATATCTCT+GGG - chr7.2:7230452-7230471 MS.gene23059:intron 35.0%
TTGTATTAGGCTTTACGTGA+GGG - chr7.2:7230706-7230725 MS.gene23059:intron 35.0%
TTTGTATTAGGCTTTACGTG+AGG - chr7.2:7230705-7230724 MS.gene23059:intron 35.0%
! CAAAGTATTAAGGGCATGTT+TGG + chr7.2:7230322-7230341 None:intergenic 35.0%
! CATCACTCCACAAAGTATTA+AGG + chr7.2:7230332-7230351 None:intergenic 35.0%
! GTTTTTGTGAGACTGTTGTT+TGG - chr7.2:7230039-7230058 MS.gene23059:CDS 35.0%
!!! GATATATGCCATTTTCTTGC+CGG + chr7.2:7230940-7230959 None:intergenic 35.0%
AAAATTGGGAGGACCAACAT+AGG + chr7.2:7230356-7230375 None:intergenic 40.0%
AACCCATTACCGTTGCATAT+GGG - chr7.2:7230986-7231005 MS.gene23059:intron 40.0%
AATTGAAGGAGTGAACACCA+AGG - chr7.2:7230896-7230915 MS.gene23059:intron 40.0%
ACAATACCACTGTTACCATG+AGG + chr7.2:7231026-7231045 None:intergenic 40.0%
ACATCTCTGGTCCAAATTGA+AGG - chr7.2:7230882-7230901 MS.gene23059:intron 40.0%
ACTGATGTCTAATGCCTGTT+TGG - chr7.2:7229984-7230003 MS.gene23059:CDS 40.0%
ATTTGGACCAGAGATGTGTT+TGG + chr7.2:7230879-7230898 None:intergenic 40.0%
GAACTTGTGAAAACAGCTCA+TGG - chr7.2:7230063-7230082 MS.gene23059:CDS 40.0%
GAGAACTTGTGAAAATCAGC+TGG + chr7.2:7230109-7230128 None:intergenic 40.0%
GTCGCTAGTATGAATATCTC+TGG - chr7.2:7230451-7230470 MS.gene23059:intron 40.0%
TGGTGTTCACTCCTTCAATT+TGG + chr7.2:7230896-7230915 None:intergenic 40.0%
TGTATTAGGCTTTACGTGAG+GGG - chr7.2:7230707-7230726 MS.gene23059:intron 40.0%
TGTCTAATGCCTGTTTGGAT+TGG - chr7.2:7229989-7230008 MS.gene23059:CDS 40.0%
TTATAAAAATGTCGGGGACC+AGG + chr7.2:7230419-7230438 None:intergenic 40.0%
TTTGGACCAGAGATGTGTTT+GGG + chr7.2:7230878-7230897 None:intergenic 40.0%
! GTCCCCGACATTTTTATAAC+TGG - chr7.2:7230420-7230439 MS.gene23059:intron 40.0%
!! GAATATCTCTGGGCTACTTT+TGG - chr7.2:7230462-7230481 MS.gene23059:intron 40.0%
!! GTTTGGGTACTGGTTTACTT+TGG + chr7.2:7230862-7230881 None:intergenic 40.0%
AAGGCCAAGGTGAAGAAGAA+CGG - chr7.2:7230963-7230982 MS.gene23059:intron 45.0%
ACCCATTACCGTTGCATATG+GGG - chr7.2:7230987-7231006 MS.gene23059:intron 45.0%
ATACCAAGCTACCTCTTCCT+TGG + chr7.2:7230916-7230935 None:intergenic 45.0%
GAACCCATTACCGTTGCATA+TGG - chr7.2:7230985-7231004 MS.gene23059:intron 45.0%
GGCATATATCTACAAGGCCA+AGG - chr7.2:7230950-7230969 MS.gene23059:intron 45.0%
TAACCTTGCCCCATATGCAA+CGG + chr7.2:7230998-7231017 None:intergenic 45.0%
TTGAGTGTGGATGCATTGCA+GGG - chr7.2:7231149-7231168 MS.gene23059:intron 45.0%
TTGGTATGCCGGCAAGAAAA+TGG - chr7.2:7230929-7230948 MS.gene23059:intron 45.0%
TTTGAGTGTGGATGCATTGC+AGG - chr7.2:7231148-7231167 MS.gene23059:intron 45.0%
ACAAGGCCTCATGGTAACAG+TGG - chr7.2:7231017-7231036 MS.gene23059:intron 50.0%
ACACCAAGGAAGAGGTAGCT+TGG - chr7.2:7230910-7230929 MS.gene23059:intron 50.0%
AGGAGTGAACACCAAGGAAG+AGG - chr7.2:7230902-7230921 MS.gene23059:intron 50.0%
CCAGAGATGTGTTTGGGTAC+TGG + chr7.2:7230872-7230891 None:intergenic 50.0%
CCAGTACCCAAACACATCTC+TGG - chr7.2:7230869-7230888 MS.gene23059:intron 50.0%
GAAGAGGTAGCTTGGTATGC+CGG - chr7.2:7230918-7230937 MS.gene23059:intron 50.0%
GCATATGGGGCAAGGTTACA+AGG - chr7.2:7231000-7231019 MS.gene23059:intron 50.0%
GCCCCATATGCAACGGTAAT+GGG + chr7.2:7230991-7231010 None:intergenic 50.0%
GGTTCCGTTCTTCTTCACCT+TGG + chr7.2:7230970-7230989 None:intergenic 50.0%
TGCCCCATATGCAACGGTAA+TGG + chr7.2:7230992-7231011 None:intergenic 50.0%
TGTGGAGTGATGGCCTATGT+TGG - chr7.2:7230340-7230359 MS.gene23059:intron 50.0%
TTACCGTTGCATATGGGGCA+AGG - chr7.2:7230992-7231011 MS.gene23059:intron 50.0%
! TACGTGAGGGGTACAATCCT+TGG - chr7.2:7230719-7230738 MS.gene23059:intron 50.0%
GGCAAGGTTACAAGGCCTCA+TGG - chr7.2:7231008-7231027 MS.gene23059:intron 55.0%
! GACGTCAAGGAGAGCGTGTC+AGG - chr7.2:7229738-7229757 MS.gene23059:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 7229728 7231211 7229728 ID=MS.gene23059
chr7.2 mRNA 7229728 7231211 7229728 ID=MS.gene23059.t1;Parent=MS.gene23059
chr7.2 exon 7231180 7231211 7231180 ID=MS.gene23059.t1.exon1;Parent=MS.gene23059.t1
chr7.2 CDS 7231180 7231211 7231180 ID=cds.MS.gene23059.t1;Parent=MS.gene23059.t1
chr7.2 exon 7230189 7230224 7230189 ID=MS.gene23059.t1.exon2;Parent=MS.gene23059.t1
chr7.2 CDS 7230189 7230224 7230189 ID=cds.MS.gene23059.t1;Parent=MS.gene23059.t1
chr7.2 exon 7229854 7230082 7229854 ID=MS.gene23059.t1.exon3;Parent=MS.gene23059.t1
chr7.2 CDS 7229854 7230082 7229854 ID=cds.MS.gene23059.t1;Parent=MS.gene23059.t1
chr7.2 exon 7229728 7229769 7229728 ID=MS.gene23059.t1.exon4;Parent=MS.gene23059.t1
chr7.2 CDS 7229728 7229769 7229728 ID=cds.MS.gene23059.t1;Parent=MS.gene23059.t1
Gene Sequence

>MS.gene23059

ATGGTGAAGGGACGTCAAGGAGAGCGTGTCAGGTACTCTTCGTTTCAGCCATTTTTATTTGTATTTTCATCATCATCGTATATGTTTTTGATATTTAGTTTAGTAGTAATTATCATTGTTTTAATTCATTTGGCTATAGCTAGTTACTACCATTCAAACATTAGCATGCAGTTAATAAGGGATTTGAATAGTATTAAGAAAAAACATCGAGATCAATTTGTTGCTTGAGAAATGTTTTAGACTATTTTGATTTGTGACTGATGTCTAATGCCTGTTTGGATTGGCTTATTTGAGCTTATCCACCAACATAAGTTTTTGTGAGACTGTTGTTTGGAGAACTTGTGAAAACAGCTCATGGCAATTATCACAGACTTTTTCCCAGCTGATTTTCACAAGTTCTCAGATATCACCTACTTCTCTCTCTCTTTTTTTTTTTTTTTTGTTGACAAAGAGTTTTCTTAAATAAAACCTATAAAAACAGCTCATAGCATATACAAAAATAATTTACCTTTATTTTTTCCTTTTCTATAAAAATAGCTTTATGTTCTCATGATAAGTGCTTGTGCTATAAGCTGCAAATAAGCAGTTTATCCAAACATGCCCTTAATACTTTGTGGAGTGATGGCCTATGTTGGTCCTCCCAATTTTTTAGCAGCTATATTTAATCCTGACAAAAATGAAATCTGAACCTGGTCCCCGACATTTTTATAACTGGACTAATTTGTCGCTAGTATGAATATCTCTGGGCTACTTTTGGTTTTATTTTTGTTAGGGATTAAATTGGGTCGCAAAATATTGTGGGACGAAAATGGGTCTTCACTCTAGTATGTGCTACTGAATTGATAATTTGATTTCTTATGACCAAATATGTTTTTGTGTCTTTTACTCAAAGATCAGTCTGTGTATCTATCTTATTTTTTATGTATCTTTATTTTATCAAATATATAAAACAAGCTAATATATACTTTCTTGTTCAATTTGTATTAGGCTTTACGTGAGGGGTACAATCCTTGGATACAAAAGGTAAACTTTTCTAATGTCATCTGTTTTATTTTATGTTTGGTTTATGAAAATGTCTGGTTAGATGCTTTGGCTTAATTAATACTTACTGTTTTATGCTGTTTTTTAGATCCAAAGTAAACCAGTACCCAAACACATCTCTGGTCCAAATTGAAGGAGTGAACACCAAGGAAGAGGTAGCTTGGTATGCCGGCAAGAAAATGGCATATATCTACAAGGCCAAGGTGAAGAAGAACGGAACCCATTACCGTTGCATATGGGGCAAGGTTACAAGGCCTCATGGTAACAGTGGTATTGTTCGTGCCAAATTCAAATCAAATCTTCCTCCCAAATCAATGGTAATAATTTACCAACACATTTTTATTCTTATTAATTGTGTTTAATAACGTTATCTTGACTTTTTGAGTGTGGATGCATTGCAGGGATCTCGCGTAAGAGTATTTATGTACCCAAGTAACATATGA

Protein sequence

>MS.gene23059.t1

MVKGRQGERVRLYVRGTILGYKRSKVNQYPNTSLVQIEGVNTKEEVAWYAGKKMAYIYKAKVKKNGTHYRCIWGKVTRPHGNSGIVRAKFKSNLPPKSMGSRVRVFMYPSNI