AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene29581


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29581.t1 MTR_5g081705 100.000 112 0 0 1 112 26 137 1.99e-79 229
MS.gene29581.t1 MTR_0699s0020 94.643 112 6 0 1 112 1 112 3.79e-77 223
MS.gene29581.t1 MTR_0699s0010 93.750 112 7 0 1 112 1 112 1.49e-76 221
MS.gene29581.t1 MTR_5g081705 100.000 99 0 0 1 99 26 124 3.59e-69 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene29581.t1 AT1G74270 88.393 112 13 0 1 112 1 112 9.66e-73 212
MS.gene29581.t1 AT1G07070 87.500 112 14 0 1 112 1 112 9.87e-73 212
MS.gene29581.t1 AT3G55750 89.189 111 12 0 2 112 1 111 6.67e-72 209
MS.gene29581.t1 AT1G41880 88.288 111 13 0 2 112 1 111 9.58e-72 209
MS.gene29581.t1 AT1G41880 88.288 111 13 0 2 112 1 111 9.58e-72 209

Find 45 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGAATTACCATTGATTT+TGG 0.162742 5.2:-69758164 None:intergenic
ACACCAATCAAAATGGTTAA+AGG 0.309838 5.2:+69757104 None:intergenic
TACGTCAGGGGTACAGTTCT+TGG 0.315019 5.2:+69757293 MS.gene29581:CDS
GAAGCCACTATCGCTGCATT+TGG 0.347853 5.2:+69758075 MS.gene29581:CDS
ATTTGTTTGTGCAGGGAGCC+AGG 0.348132 5.2:+69758257 MS.gene29581:intron
AAGCCACTATCGCTGCATTT+GGG 0.372191 5.2:+69758076 MS.gene29581:CDS
TTGTAATCAGGCTTTACGTC+AGG 0.401581 5.2:+69757279 MS.gene29581:intron
ATGTATCCAAGCAATATATA+AGG 0.407008 5.2:+69758292 MS.gene29581:CDS
ATCTGAACAAGAGAGGTGTT+TGG 0.416693 5.2:-69757966 None:intergenic
GACGACAAGGAGAACGTATC+AGG 0.417479 5.2:+69757126 MS.gene29581:CDS
GAGGAGGTTGCATGGTATGC+TGG 0.417733 5.2:+69758008 MS.gene29581:CDS
CTATCGCTGCATTTGGGGTA+AGG 0.428656 5.2:+69758082 MS.gene29581:CDS
ACATTACCTTATATATTGCT+TGG 0.430770 5.2:-69758298 None:intergenic
TCTGAACAAGAGAGGTGTTT+GGG 0.453404 5.2:-69757965 None:intergenic
TACCCCAAATGCAGCGATAG+TGG 0.457668 5.2:-69758079 None:intergenic
GTTTGGGTATTGATTTACCT+TGG 0.466066 5.2:-69757949 None:intergenic
ATGGTATGCTGGGAAGAAAT+TGG 0.469927 5.2:+69758019 MS.gene29581:CDS
GCTTCCATCCTTCTTCACCT+TGG 0.472167 5.2:-69758057 None:intergenic
ACCTCTCTTGTTCAGATCGA+GGG 0.499812 5.2:+69757972 MS.gene29581:CDS
GCATTTGGGGTAAGGTTACC+AGG 0.512393 5.2:+69758090 MS.gene29581:CDS
ATACCATGCAACCTCCTCCT+TGG 0.519241 5.2:-69758003 None:intergenic
GCCACTGTTACCATGAGGCC+TGG 0.523824 5.2:-69758108 None:intergenic
AGGAGGTTGCATGGTATGCT+GGG 0.527652 5.2:+69758009 MS.gene29581:CDS
TTTGTTTGTGCAGGGAGCCA+GGG 0.534698 5.2:+69758258 MS.gene29581:intron
TTACAAGGCCAAGGTGAAGA+AGG 0.537680 5.2:+69758049 MS.gene29581:CDS
TAACCTACCACCAAAATCAA+TGG 0.539094 5.2:+69758154 MS.gene29581:CDS
GGCATACATTTACAAGGCCA+AGG 0.547418 5.2:+69758040 MS.gene29581:CDS
TGTAATCAGGCTTTACGTCA+GGG 0.561952 5.2:+69757280 MS.gene29581:intron
GAAATTGGCATACATTTACA+AGG 0.562532 5.2:+69758034 MS.gene29581:CDS
GTAATGTTGTTATAGGTCCA+AGG 0.573819 5.2:+69757932 MS.gene29581:intron
ATACATAAATACTCTAACCC+TGG 0.583670 5.2:-69758275 None:intergenic
GGTAAGGTTACCAGGCCTCA+TGG 0.585246 5.2:+69758098 MS.gene29581:CDS
AAGGCCAAGGTGAAGAAGGA+TGG 0.591334 5.2:+69758053 MS.gene29581:CDS
CACCTCTCTTGTTCAGATCG+AGG 0.592183 5.2:+69757971 MS.gene29581:CDS
ACACCAAGGAGGAGGTTGCA+TGG 0.606757 5.2:+69758000 MS.gene29581:CDS
GTAATCAGGCTTTACGTCAG+GGG 0.611028 5.2:+69757281 MS.gene29581:intron
GGTTAGACTTGAACTTTGCG+CGG 0.621246 5.2:-69758136 None:intergenic
AGCCACTATCGCTGCATTTG+GGG 0.627011 5.2:+69758077 MS.gene29581:CDS
CCTCTCTTGTTCAGATCGAG+GGG 0.637714 5.2:+69757973 MS.gene29581:CDS
GTACAGTTCTTGGATACAAG+AGG 0.641528 5.2:+69757303 MS.gene29581:CDS
CCCCTCGATCTGAACAAGAG+AGG 0.655801 5.2:-69757973 None:intergenic
ACCAGGCCTCATGGTAACAG+TGG 0.661908 5.2:+69758107 MS.gene29581:CDS
AAAATGGTTAAAGGACGACA+AGG 0.679088 5.2:+69757113 None:intergenic
CTCTCTTGTTCAGATCGAGG+GGG 0.711001 5.2:+69757974 MS.gene29581:CDS
ACAATGCCACTGTTACCATG+AGG 0.746625 5.2:-69758113 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGCTAATTAAATCTAATTT+GGG + chr5.2:69757415-69757434 MS.gene29581:intron 15.0%
!! TAAGCTAATTAAATCTAATT+TGG + chr5.2:69757414-69757433 MS.gene29581:intron 15.0%
!!! AATTCTTTAAGACATTTAAA+TGG + chr5.2:69758178-69758197 MS.gene29581:intron 15.0%
!!! ATTTAATTAGCTTAAGATAT+TGG - chr5.2:69757408-69757427 None:intergenic 15.0%
!! CGGAAAAAAAACTATATATT+TGG - chr5.2:69757828-69757847 None:intergenic 20.0%
!!! ATATTTTATCAAGGATACAT+TGG - chr5.2:69757665-69757684 None:intergenic 20.0%
!!! TAAAGAATTACCATTGATTT+TGG - chr5.2:69758167-69758186 None:intergenic 20.0%
!!! TATTTTATCAAGGATACATT+GGG - chr5.2:69757664-69757683 None:intergenic 20.0%
!!! TTGGGTATTATATTTTATCA+AGG - chr5.2:69757674-69757693 None:intergenic 20.0%
!!! TTTGGGTATTATTTTTTGTA+TGG + chr5.2:69757769-69757788 MS.gene29581:intron 20.0%
! ACAAACACAAATTAAAAGCT+GGG - chr5.2:69757587-69757606 None:intergenic 25.0%
! ATCAAAATCAAGAACTACTA+GGG - chr5.2:69757871-69757890 None:intergenic 25.0%
! GATTATTTGTGCTAAGAAAT+TGG + chr5.2:69757910-69757929 MS.gene29581:intron 25.0%
! TTAAAAACCAAGACTCAATT+TGG + chr5.2:69757618-69757637 MS.gene29581:intron 25.0%
!! TTGATTTTGTGTTTGTAATC+AGG + chr5.2:69757267-69757286 MS.gene29581:intron 25.0%
!!! GAAATTGGTAATGTTGTTAT+AGG + chr5.2:69757925-69757944 MS.gene29581:intron 25.0%
!!! GACTAAATGATTACTGATTT+GGG + chr5.2:69757752-69757771 MS.gene29581:intron 25.0%
!!! TTTATGGTTTCTGAAAGTTT+TGG + chr5.2:69758229-69758248 MS.gene29581:intron 25.0%
ATACATAAATACTCTAACCC+TGG - chr5.2:69758278-69758297 None:intergenic 30.0%
CACAAACACAAATTAAAAGC+TGG - chr5.2:69757588-69757607 None:intergenic 30.0%
CATCAAAATCAAGAACTACT+AGG - chr5.2:69757872-69757891 None:intergenic 30.0%
GAAATTGGCATACATTTACA+AGG + chr5.2:69758034-69758053 MS.gene29581:CDS 30.0%
GCTGTATTTGATGTATTCTT+GGG + chr5.2:69757362-69757381 MS.gene29581:intron 30.0%
GTTAAACGAAATAGATCGAA+GGG - chr5.2:69757164-69757183 None:intergenic 30.0%
TGCTGTATTTGATGTATTCT+TGG + chr5.2:69757361-69757380 MS.gene29581:intron 30.0%
TGTTAAACGAAATAGATCGA+AGG - chr5.2:69757165-69757184 None:intergenic 30.0%
TTCAGAAACCATAAAACAGT+TGG - chr5.2:69758224-69758243 None:intergenic 30.0%
! GGACTAAATGATTACTGATT+TGG + chr5.2:69757751-69757770 MS.gene29581:intron 30.0%
!! CTTGATTTTGATGTTGATCT+GGG + chr5.2:69757879-69757898 MS.gene29581:intron 30.0%
!! TCTTGATTTTGATGTTGATC+TGG + chr5.2:69757878-69757897 MS.gene29581:intron 30.0%
!!! AGAATTACCATTGATTTTGG+TGG - chr5.2:69758164-69758183 None:intergenic 30.0%
!!! ATGTTTAATGCTGTTTTAGC+TGG + chr5.2:69757502-69757521 MS.gene29581:intron 30.0%
GTAATGTTGTTATAGGTCCA+AGG + chr5.2:69757932-69757951 MS.gene29581:intron 35.0%
TAACCTACCACCAAAATCAA+TGG + chr5.2:69758154-69758173 MS.gene29581:CDS 35.0%
TGAAGACACATTATGTACGA+CGG - chr5.2:69757848-69757867 None:intergenic 35.0%
TTGGTCCTTCACAATATTTG+CGG + chr5.2:69757637-69757656 MS.gene29581:intron 35.0%
! CCTTTTCACCAACTGTTTTA+TGG + chr5.2:69758213-69758232 MS.gene29581:intron 35.0%
!! CCATAAAACAGTTGGTGAAA+AGG - chr5.2:69758216-69758235 None:intergenic 35.0%
!! GTTTGGGTATTGATTTACCT+TGG - chr5.2:69757952-69757971 None:intergenic 35.0%
!!! TTACCATTGATTTTGGTGGT+AGG - chr5.2:69758160-69758179 None:intergenic 35.0%
ATGGTATGCTGGGAAGAAAT+TGG + chr5.2:69758019-69758038 MS.gene29581:CDS 40.0%
CCTAGACTCATTATCACAAG+CGG - chr5.2:69757733-69757752 None:intergenic 40.0%
GTACAGTTCTTGGATACAAG+AGG + chr5.2:69757303-69757322 MS.gene29581:CDS 40.0%
TGAAGGACCAAATTGAGTCT+TGG - chr5.2:69757628-69757647 None:intergenic 40.0%
TGGTCCTTCACAATATTTGC+GGG + chr5.2:69757638-69757657 MS.gene29581:intron 40.0%
TGTAATCAGGCTTTACGTCA+GGG + chr5.2:69757280-69757299 MS.gene29581:intron 40.0%
TTGTAATCAGGCTTTACGTC+AGG + chr5.2:69757279-69757298 MS.gene29581:intron 40.0%
!! ATCTGAACAAGAGAGGTGTT+TGG - chr5.2:69757969-69757988 None:intergenic 40.0%
!! GATAATGCTGGGACTAGTTT+GGG - chr5.2:69757692-69757711 None:intergenic 40.0%
!! GGTGTTGATTTGTTTGTGCA+GGG + chr5.2:69758250-69758269 MS.gene29581:intron 40.0%
!! TCTGAACAAGAGAGGTGTTT+GGG - chr5.2:69757968-69757987 None:intergenic 40.0%
!! TGATAATGCTGGGACTAGTT+TGG - chr5.2:69757693-69757712 None:intergenic 40.0%
!! TGGTGTTGATTTGTTTGTGC+AGG + chr5.2:69758249-69758268 MS.gene29581:intron 40.0%
AAGCCACTATCGCTGCATTT+GGG + chr5.2:69758076-69758095 MS.gene29581:CDS 45.0%
ACAATGCCACTGTTACCATG+AGG - chr5.2:69758116-69758135 None:intergenic 45.0%
ACCTCTCTTGTTCAGATCGA+GGG + chr5.2:69757972-69757991 MS.gene29581:CDS 45.0%
GGCATACATTTACAAGGCCA+AGG + chr5.2:69758040-69758059 MS.gene29581:CDS 45.0%
GGTTAGACTTGAACTTTGCG+CGG - chr5.2:69758139-69758158 None:intergenic 45.0%
GTAATCAGGCTTTACGTCAG+GGG + chr5.2:69757281-69757300 MS.gene29581:intron 45.0%
TCCCAGCATTATCACAGTAC+AGG + chr5.2:69757699-69757718 MS.gene29581:intron 45.0%
TTACAAGGCCAAGGTGAAGA+AGG + chr5.2:69758049-69758068 MS.gene29581:CDS 45.0%
TTGATGTTGATCTGGGTTGC+TGG + chr5.2:69757886-69757905 MS.gene29581:intron 45.0%
! CCGCTTGTGATAATGAGTCT+AGG + chr5.2:69757730-69757749 MS.gene29581:intron 45.0%
! GCCTGTACTGTGATAATGCT+GGG - chr5.2:69757703-69757722 None:intergenic 45.0%
! TGCCTGTACTGTGATAATGC+TGG - chr5.2:69757704-69757723 None:intergenic 45.0%
AAGGCCAAGGTGAAGAAGGA+TGG + chr5.2:69758053-69758072 MS.gene29581:CDS 50.0%
AGCCACTATCGCTGCATTTG+GGG + chr5.2:69758077-69758096 MS.gene29581:CDS 50.0%
AGGAGGTTGCATGGTATGCT+GGG + chr5.2:69758009-69758028 MS.gene29581:CDS 50.0%
ATACCATGCAACCTCCTCCT+TGG - chr5.2:69758006-69758025 None:intergenic 50.0%
ATTTGTTTGTGCAGGGAGCC+AGG + chr5.2:69758257-69758276 MS.gene29581:intron 50.0%
CACCTCTCTTGTTCAGATCG+AGG + chr5.2:69757971-69757990 MS.gene29581:CDS 50.0%
CCTCTCTTGTTCAGATCGAG+GGG + chr5.2:69757973-69757992 MS.gene29581:CDS 50.0%
CTCTCTTGTTCAGATCGAGG+GGG + chr5.2:69757974-69757993 MS.gene29581:CDS 50.0%
GAAGCCACTATCGCTGCATT+TGG + chr5.2:69758075-69758094 MS.gene29581:CDS 50.0%
GACGACAAGGAGAACGTATC+AGG + chr5.2:69757126-69757145 MS.gene29581:CDS 50.0%
GCTTCCATCCTTCTTCACCT+TGG - chr5.2:69758060-69758079 None:intergenic 50.0%
TACCCCAAATGCAGCGATAG+TGG - chr5.2:69758082-69758101 None:intergenic 50.0%
TGGGCCCGCAAATATTGTGA+AGG - chr5.2:69757645-69757664 None:intergenic 50.0%
TTTGTTTGTGCAGGGAGCCA+GGG + chr5.2:69758258-69758277 MS.gene29581:intron 50.0%
! CTATCGCTGCATTTGGGGTA+AGG + chr5.2:69758082-69758101 MS.gene29581:CDS 50.0%
! GCATTTGGGGTAAGGTTACC+AGG + chr5.2:69758090-69758109 MS.gene29581:CDS 50.0%
! TACGTCAGGGGTACAGTTCT+TGG + chr5.2:69757293-69757312 MS.gene29581:CDS 50.0%
ACACCAAGGAGGAGGTTGCA+TGG + chr5.2:69758000-69758019 MS.gene29581:CDS 55.0%
ACCAGGCCTCATGGTAACAG+TGG + chr5.2:69758107-69758126 MS.gene29581:CDS 55.0%
CCCCTCGATCTGAACAAGAG+AGG - chr5.2:69757976-69757995 None:intergenic 55.0%
GAGGAGGTTGCATGGTATGC+TGG + chr5.2:69758008-69758027 MS.gene29581:CDS 55.0%
GATCGAGGGGGTAAACACCA+AGG + chr5.2:69757986-69758005 MS.gene29581:CDS 55.0%
GGTAAGGTTACCAGGCCTCA+TGG + chr5.2:69758098-69758117 MS.gene29581:CDS 55.0%
CGAGGGGGTAAACACCAAGG+AGG + chr5.2:69757989-69758008 MS.gene29581:CDS 60.0%
GCCACTGTTACCATGAGGCC+TGG - chr5.2:69758111-69758130 None:intergenic 60.0%
GGGGGTAAACACCAAGGAGG+AGG + chr5.2:69757992-69758011 MS.gene29581:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 69757116 69758312 69757116 ID=MS.gene29581
chr5.2 mRNA 69757116 69758312 69757116 ID=MS.gene29581.t1;Parent=MS.gene29581
chr5.2 exon 69757116 69757147 69757116 ID=MS.gene29581.t1.exon1;Parent=MS.gene29581.t1
chr5.2 CDS 69757116 69757147 69757116 ID=cds.MS.gene29581.t1;Parent=MS.gene29581.t1
chr5.2 exon 69757289 69757324 69757289 ID=MS.gene29581.t1.exon2;Parent=MS.gene29581.t1
chr5.2 CDS 69757289 69757324 69757289 ID=cds.MS.gene29581.t1;Parent=MS.gene29581.t1
chr5.2 exon 69757947 69758175 69757947 ID=MS.gene29581.t1.exon3;Parent=MS.gene29581.t1
chr5.2 CDS 69757947 69758175 69757947 ID=cds.MS.gene29581.t1;Parent=MS.gene29581.t1
chr5.2 exon 69758271 69758312 69758271 ID=MS.gene29581.t1.exon4;Parent=MS.gene29581.t1
chr5.2 CDS 69758271 69758312 69758271 ID=cds.MS.gene29581.t1;Parent=MS.gene29581.t1
Gene Sequence

>MS.gene29581

ATGGTTAAAGGACGACAAGGAGAACGTATCAGGTTTCTCTTCTTACCCTTCGATCTATTTCGTTTAACACGAATTTGAGCTAGTTTTCATGATTAAAATTGTAATTTGTATAGTTAATCATATTTAATGAGTATTTACTAATGTTGTTATTTTGATTTTGTGTTTGTAATCAGGCTTTACGTCAGGGGTACAGTTCTTGGATACAAGAGGTAAAAGTTTTTCTTCTCGATTTATTTTTCAGTTTTTGCTGTATTTGATGTATTCTTGGGTTTTCTTAAGTTAATGAAATCCAATATCTTAAGCTAATTAAATCTAATTTGGGTTTATAATTATATAGAAATATGTAATGTATGATTTGTTAAGCTGAGCTTTATTTGTGCTAAGAAATGTTTAATGCTGTTTTAGCTGGATATGTTGTTCTTTGAATTTTGTAGTTTATGTTAAGTTTTTAAAGTTTTCTGAAGTAAACCCAGCTTTTAATTTGTGTTTGTGATTCATAAATTTAAAAACCAAGACTCAATTTGGTCCTTCACAATATTTGCGGGCCCAATGTATCCTTGATAAAATATAATACCCAAACTAGTCCCAGCATTATCACAGTACAGGCAATCAATCCGCTTGTGATAATGAGTCTAGGACTAAATGATTACTGATTTGGGTATTATTTTTTGTATGGAGCTAGATTAAGCAGTCGCAAAATTACGAGAGACCAAATATATAGTTTTTTTTCCGTCGTACATAATGTGTCTTCACCCTAGTAGTTCTTGATTTTGATGTTGATCTGGGTTGCTGGTGATTATTTGTGCTAAGAAATTGGTAATGTTGTTATAGGTCCAAGGTAAATCAATACCCAAACACCTCTCTTGTTCAGATCGAGGGGGTAAACACCAAGGAGGAGGTTGCATGGTATGCTGGGAAGAAATTGGCATACATTTACAAGGCCAAGGTGAAGAAGGATGGAAGCCACTATCGCTGCATTTGGGGTAAGGTTACCAGGCCTCATGGTAACAGTGGCATTGTCCGCGCAAAGTTCAAGTCTAACCTACCACCAAAATCAATGGTAATTCTTTAAGACATTTAAATGGTTTTTTTCTTTTCCTTTTCACCAACTGTTTTATGGTTTCTGAAAGTTTTGGTGTTGATTTGTTTGTGCAGGGAGCCAGGGTTAGAGTATTTATGTATCCAAGCAATATATAA

Protein sequence

>MS.gene29581.t1

MVKGRQGERIRLYVRGTVLGYKRSKVNQYPNTSLVQIEGVNTKEEVAWYAGKKLAYIYKAKVKKDGSHYRCIWGKVTRPHGNSGIVRAKFKSNLPPKSMGARVRVFMYPSNI