Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070073.t1 | XP_013454243.1 | 100 | 112 | 0 | 0 | 1 | 112 | 26 | 137 | 1.10E-57 | 232.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070073.t1 | Q9LMK0 | 87.5 | 112 | 14 | 0 | 1 | 112 | 1 | 112 | 7.7e-55 | 214.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070073.t1 | A0A072UET3 | 100.0 | 112 | 0 | 0 | 1 | 112 | 26 | 137 | 8.2e-58 | 232.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene051904 | MS.gene070073 | PPI |
| MS.gene23058 | MS.gene070073 | PPI |
| MS.gene070073 | MS.gene23059 | PPI |
| MS.gene070073 | MS.gene39294 | PPI |
| MS.gene56696 | MS.gene070073 | PPI |
| MS.gene29581 | MS.gene070073 | PPI |
| MS.gene061905 | MS.gene070073 | PPI |
| MS.gene007067 | MS.gene070073 | PPI |
| MS.gene070073 | MS.gene022752 | PPI |
| MS.gene070073 | MS.gene64512 | PPI |
| MS.gene007066 | MS.gene070073 | PPI |
| MS.gene070073 | MS.gene020215 | PPI |
| MS.gene09845 | MS.gene070073 | PPI |
| MS.gene018553 | MS.gene070073 | PPI |
| MS.gene070073 | MS.gene80639 | PPI |
| MS.gene45646 | MS.gene070073 | PPI |
| MS.gene051903 | MS.gene070073 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070073.t1 | MTR_5g081705 | 100.000 | 112 | 0 | 0 | 1 | 112 | 26 | 137 | 1.99e-79 | 229 |
| MS.gene070073.t1 | MTR_0699s0020 | 94.643 | 112 | 6 | 0 | 1 | 112 | 1 | 112 | 3.79e-77 | 223 |
| MS.gene070073.t1 | MTR_0699s0010 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 1.49e-76 | 221 |
| MS.gene070073.t1 | MTR_5g081705 | 100.000 | 99 | 0 | 0 | 1 | 99 | 26 | 124 | 3.59e-69 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070073.t1 | AT1G74270 | 88.393 | 112 | 13 | 0 | 1 | 112 | 1 | 112 | 9.66e-73 | 212 |
| MS.gene070073.t1 | AT1G07070 | 87.500 | 112 | 14 | 0 | 1 | 112 | 1 | 112 | 9.87e-73 | 212 |
| MS.gene070073.t1 | AT3G55750 | 89.189 | 111 | 12 | 0 | 2 | 112 | 1 | 111 | 6.67e-72 | 209 |
| MS.gene070073.t1 | AT1G41880 | 88.288 | 111 | 13 | 0 | 2 | 112 | 1 | 111 | 9.58e-72 | 209 |
| MS.gene070073.t1 | AT1G41880 | 88.288 | 111 | 13 | 0 | 2 | 112 | 1 | 111 | 9.58e-72 | 209 |
Find 41 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACCAATCAAAATGGTTAA+AGG | 0.315193 | 5.3:+66138068 | None:intergenic |
| TATGTCAGGGGCACAGTTCT+TGG | 0.316095 | 5.3:+66138215 | MS.gene070073:CDS |
| TTACAAGGCCAAGGTGAAAA+AGG | 0.324778 | 5.3:+66138910 | MS.gene070073:CDS |
| GTAGCCACTATCGCTGCATT+TGG | 0.329880 | 5.3:+66138936 | MS.gene070073:CDS |
| ATTTGTTTGTGCAGGGAGCC+AGG | 0.348132 | 5.3:+66139118 | MS.gene070073:intron |
| TAGCCACTATCGCTGCATTT+GGG | 0.375359 | 5.3:+66138937 | MS.gene070073:CDS |
| ATGTATCCAAGCAATATATA+AGG | 0.407008 | 5.3:+66139153 | MS.gene070073:CDS |
| AAATTACCTTATATATTGCT+TGG | 0.412163 | 5.3:-66139159 | None:intergenic |
| ATCTGAACAAGAGAGGTGTT+TGG | 0.416693 | 5.3:-66138827 | None:intergenic |
| GAGGAGGTTGCATGGTATGC+TGG | 0.417733 | 5.3:+66138869 | MS.gene070073:CDS |
| CTATCGCTGCATTTGGGGTA+AGG | 0.428656 | 5.3:+66138943 | MS.gene070073:CDS |
| TCTGAACAAGAGAGGTGTTT+GGG | 0.453404 | 5.3:-66138826 | None:intergenic |
| GACGTCAAGGAGAACGCATC+AGG | 0.461646 | 5.3:+66138090 | MS.gene070073:CDS |
| TACCCCAAATGCAGCGATAG+TGG | 0.462856 | 5.3:-66138940 | None:intergenic |
| GTTTGGGTATTGATTTACCT+TGG | 0.466066 | 5.3:-66138810 | None:intergenic |
| ATGGTATGCTGGGAAGAAAT+TGG | 0.469927 | 5.3:+66138880 | MS.gene070073:CDS |
| ACCTCTCTTGTTCAGATCGA+GGG | 0.499812 | 5.3:+66138833 | MS.gene070073:CDS |
| GCATTTGGGGTAAGGTTACC+AGG | 0.512393 | 5.3:+66138951 | MS.gene070073:CDS |
| ATACCATGCAACCTCCTCCT+TGG | 0.519241 | 5.3:-66138864 | None:intergenic |
| AGGAGGTTGCATGGTATGCT+GGG | 0.527652 | 5.3:+66138870 | MS.gene070073:CDS |
| TTTGTTTGTGCAGGGAGCCA+GGG | 0.534698 | 5.3:+66139119 | MS.gene070073:intron |
| TTTAATCAGGCTTTATGTCA+GGG | 0.536616 | 5.3:+66138202 | MS.gene070073:intron |
| TAACCTACCACCAAAATCAA+TGG | 0.539094 | 5.3:+66139015 | MS.gene070073:CDS |
| GTAATGTTGTTATAGGTCCA+AGG | 0.544802 | 5.3:+66138793 | MS.gene070073:intron |
| ACCACTGTTACCATGAGGCC+TGG | 0.545885 | 5.3:-66138969 | None:intergenic |
| GGCATACATTTACAAGGCCA+AGG | 0.547418 | 5.3:+66138901 | MS.gene070073:CDS |
| GAAATTGGCATACATTTACA+AGG | 0.562532 | 5.3:+66138895 | MS.gene070073:CDS |
| ATACATAAATACTCTAACCC+TGG | 0.583670 | 5.3:-66139136 | None:intergenic |
| GGTAAGGTTACCAGGCCTCA+TGG | 0.585246 | 5.3:+66138959 | MS.gene070073:CDS |
| CACCTCTCTTGTTCAGATCG+AGG | 0.592183 | 5.3:+66138832 | MS.gene070073:CDS |
| ACACCAAGGAGGAGGTTGCA+TGG | 0.606757 | 5.3:+66138861 | MS.gene070073:CDS |
| TTAATCAGGCTTTATGTCAG+GGG | 0.608287 | 5.3:+66138203 | MS.gene070073:intron |
| AAAATGGTTAAAGGACGTCA+AGG | 0.620787 | 5.3:+66138077 | None:intergenic |
| GGTTAGACTTGAACTTTGCG+CGG | 0.621246 | 5.3:-66138997 | None:intergenic |
| AGCCACTATCGCTGCATTTG+GGG | 0.630179 | 5.3:+66138938 | MS.gene070073:CDS |
| CCTCTCTTGTTCAGATCGAG+GGG | 0.637714 | 5.3:+66138834 | MS.gene070073:CDS |
| GCACAGTTCTTGGATACAAG+AGG | 0.637980 | 5.3:+66138225 | MS.gene070073:CDS |
| CCCCTCGATCTGAACAAGAG+AGG | 0.655801 | 5.3:-66138834 | None:intergenic |
| ACCAGGCCTCATGGTAACAG+TGG | 0.672394 | 5.3:+66138968 | MS.gene070073:CDS |
| CTCTCTTGTTCAGATCGAGG+GGG | 0.711001 | 5.3:+66138835 | MS.gene070073:CDS |
| ACAATACCACTGTTACCATG+AGG | 0.737809 | 5.3:-66138974 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACTAATTAAATCTAATTT+GGG | + | chr5.3:66138325-66138344 | MS.gene070073:intron | 10.0% |
| !! | TAAACTAATTAAATCTAATT+TGG | + | chr5.3:66138324-66138343 | MS.gene070073:intron | 10.0% |
| !!! | AATATATAATTTTTTTAGCT+GGG | + | chr5.3:66138371-66138390 | MS.gene070073:intron | 10.0% |
| !!! | TAATATATAATTTTTTTAGC+TGG | + | chr5.3:66138370-66138389 | MS.gene070073:intron | 10.0% |
| !! | AATAAAATCAAAAGAGTTAA+AGG | - | chr5.3:66138143-66138162 | None:intergenic | 15.0% |
| !! | ATTATTTGTAATGTTGTTAT+AGG | + | chr5.3:66138786-66138805 | MS.gene070073:intron | 15.0% |
| !!! | ATTTTTTGAAGACATTTAAA+TGG | + | chr5.3:66139040-66139059 | MS.gene070073:intron | 15.0% |
| !! | AGGGTGAAAAATTATATATT+TGG | - | chr5.3:66138727-66138746 | None:intergenic | 20.0% |
| !! | ATCAAAATCAAAAACTACTA+GGG | - | chr5.3:66138746-66138765 | None:intergenic | 20.0% |
| !!! | CAAAAAATTACCATTGATTT+TGG | - | chr5.3:66139028-66139047 | None:intergenic | 20.0% |
| !!! | TTCATTTTGTGTTTTTAATC+AGG | + | chr5.3:66138189-66138208 | MS.gene070073:intron | 20.0% |
| ! | CATCAAAATCAAAAACTACT+AGG | - | chr5.3:66138747-66138766 | None:intergenic | 25.0% |
| ! | TTTAAGAACAAGACTCAATT+TGG | + | chr5.3:66138519-66138538 | MS.gene070073:intron | 25.0% |
| !! | ATATTTTATCGAGGATACAT+TGG | - | chr5.3:66138566-66138585 | None:intergenic | 25.0% |
| !! | TATTTTATCGAGGATACATT+GGG | - | chr5.3:66138565-66138584 | None:intergenic | 25.0% |
| !! | TCTGGGTATTAATTTTTGTA+TGG | + | chr5.3:66138670-66138689 | MS.gene070073:intron | 25.0% |
| !! | TTGGGTATTATATTTTATCG+AGG | - | chr5.3:66138575-66138594 | None:intergenic | 25.0% |
| !!! | AAAATTACCATTGATTTTGG+TGG | - | chr5.3:66139025-66139044 | None:intergenic | 25.0% |
| !!! | CTTTTTCACAAACTGTTTTA+TGG | + | chr5.3:66139074-66139093 | MS.gene070073:intron | 25.0% |
| !!! | GAAATGTTTAATGTTTTAGC+TGG | + | chr5.3:66138409-66138428 | MS.gene070073:intron | 25.0% |
| !!! | TTTATGGTTTCTGAAAGTTT+TGG | + | chr5.3:66139090-66139109 | MS.gene070073:intron | 25.0% |
| !!! | TTTGATTTTGATGTTGATCT+GGG | + | chr5.3:66138754-66138773 | MS.gene070073:intron | 25.0% |
| !!! | TTTTGATTTTGATGTTGATC+TGG | + | chr5.3:66138753-66138772 | MS.gene070073:intron | 25.0% |
| !!! | TTTTTTTAGCTGGGTTTTAT+TGG | + | chr5.3:66138380-66138399 | MS.gene070073:intron | 25.0% |
| ACAACCACAAATTAAAAGCT+GGG | - | chr5.3:66138489-66138508 | None:intergenic | 30.0% | |
| ATACATAAATACTCTAACCC+TGG | - | chr5.3:66139139-66139158 | None:intergenic | 30.0% | |
| GAAATTGGCATACATTTACA+AGG | + | chr5.3:66138895-66138914 | MS.gene070073:CDS | 30.0% | |
| GACTATATGATTACTGATCT+GGG | + | chr5.3:66138653-66138672 | MS.gene070073:intron | 30.0% | |
| TTGGTCCTTCACAATATTTA+CGG | + | chr5.3:66138538-66138557 | MS.gene070073:intron | 30.0% | |
| ! | TTTAATCAGGCTTTATGTCA+GGG | + | chr5.3:66138202-66138221 | MS.gene070073:intron | 30.0% |
| !! | TTTTAATCAGGCTTTATGTC+AGG | + | chr5.3:66138201-66138220 | MS.gene070073:intron | 30.0% |
| !!! | TAAACCCAGCTTTTAATTTG+TGG | + | chr5.3:66138482-66138501 | MS.gene070073:intron | 30.0% |
| AGTTAAAGGATACAGATCGA+AGG | - | chr5.3:66138129-66138148 | None:intergenic | 35.0% | |
| CACAACCACAAATTAAAAGC+TGG | - | chr5.3:66138490-66138509 | None:intergenic | 35.0% | |
| GGACTATATGATTACTGATC+TGG | + | chr5.3:66138652-66138671 | MS.gene070073:intron | 35.0% | |
| GTAATGTTGTTATAGGTCCA+AGG | + | chr5.3:66138793-66138812 | MS.gene070073:intron | 35.0% | |
| GTTAAAGGATACAGATCGAA+GGG | - | chr5.3:66138128-66138147 | None:intergenic | 35.0% | |
| TAACCTACCACCAAAATCAA+TGG | + | chr5.3:66139015-66139034 | MS.gene070073:CDS | 35.0% | |
| TGGTCCTTCACAATATTTAC+GGG | + | chr5.3:66138539-66138558 | MS.gene070073:intron | 35.0% | |
| ! | TTAATCAGGCTTTATGTCAG+GGG | + | chr5.3:66138203-66138222 | MS.gene070073:intron | 35.0% |
| !! | GATAATGCTGGGATTAGTTT+GGG | - | chr5.3:66138593-66138612 | None:intergenic | 35.0% |
| !! | GTTTGGGTATTGATTTACCT+TGG | - | chr5.3:66138813-66138832 | None:intergenic | 35.0% |
| !! | TGATAATGCTGGGATTAGTT+TGG | - | chr5.3:66138594-66138613 | None:intergenic | 35.0% |
| !!! | TTACCATTGATTTTGGTGGT+AGG | - | chr5.3:66139021-66139040 | None:intergenic | 35.0% |
| ACAATACCACTGTTACCATG+AGG | - | chr5.3:66138977-66138996 | None:intergenic | 40.0% | |
| ATGGTATGCTGGGAAGAAAT+TGG | + | chr5.3:66138880-66138899 | MS.gene070073:CDS | 40.0% | |
| CCAAGACTCATTATCACAAG+CGG | - | chr5.3:66138634-66138653 | None:intergenic | 40.0% | |
| TTACAAGGCCAAGGTGAAAA+AGG | + | chr5.3:66138910-66138929 | MS.gene070073:CDS | 40.0% | |
| !! | ATCTGAACAAGAGAGGTGTT+TGG | - | chr5.3:66138830-66138849 | None:intergenic | 40.0% |
| !! | GGTGTTGATTTGTTTGTGCA+GGG | + | chr5.3:66139111-66139130 | MS.gene070073:intron | 40.0% |
| !! | TCTGAACAAGAGAGGTGTTT+GGG | - | chr5.3:66138829-66138848 | None:intergenic | 40.0% |
| !! | TGGTGTTGATTTGTTTGTGC+AGG | + | chr5.3:66139110-66139129 | MS.gene070073:intron | 40.0% |
| AAGGCCAAGGTGAAAAAGGA+TGG | + | chr5.3:66138914-66138933 | MS.gene070073:CDS | 45.0% | |
| ACCTCTCTTGTTCAGATCGA+GGG | + | chr5.3:66138833-66138852 | MS.gene070073:CDS | 45.0% | |
| GCACAGTTCTTGGATACAAG+AGG | + | chr5.3:66138225-66138244 | MS.gene070073:CDS | 45.0% | |
| GGCATACATTTACAAGGCCA+AGG | + | chr5.3:66138901-66138920 | MS.gene070073:CDS | 45.0% | |
| GGTTAGACTTGAACTTTGCG+CGG | - | chr5.3:66139000-66139019 | None:intergenic | 45.0% | |
| TAGCCACTATCGCTGCATTT+GGG | + | chr5.3:66138937-66138956 | MS.gene070073:CDS | 45.0% | |
| TCCCAGCATTATCACAGTAC+AGG | + | chr5.3:66138600-66138619 | MS.gene070073:intron | 45.0% | |
| TGGGCCCGTAAATATTGTGA+AGG | - | chr5.3:66138546-66138565 | None:intergenic | 45.0% | |
| TTGATGTTGATCTGGGTTGC+TGG | + | chr5.3:66138761-66138780 | MS.gene070073:intron | 45.0% | |
| ! | CCGCTTGTGATAATGAGTCT+TGG | + | chr5.3:66138631-66138650 | MS.gene070073:intron | 45.0% |
| ! | GCCTGTACTGTGATAATGCT+GGG | - | chr5.3:66138604-66138623 | None:intergenic | 45.0% |
| ! | TGCCTGTACTGTGATAATGC+TGG | - | chr5.3:66138605-66138624 | None:intergenic | 45.0% |
| !! | GCTACCATCCTTTTTCACCT+TGG | - | chr5.3:66138921-66138940 | None:intergenic | 45.0% |
| AGCCACTATCGCTGCATTTG+GGG | + | chr5.3:66138938-66138957 | MS.gene070073:CDS | 50.0% | |
| AGGAGGTTGCATGGTATGCT+GGG | + | chr5.3:66138870-66138889 | MS.gene070073:CDS | 50.0% | |
| ATACCATGCAACCTCCTCCT+TGG | - | chr5.3:66138867-66138886 | None:intergenic | 50.0% | |
| ATTTGTTTGTGCAGGGAGCC+AGG | + | chr5.3:66139118-66139137 | MS.gene070073:intron | 50.0% | |
| CACCTCTCTTGTTCAGATCG+AGG | + | chr5.3:66138832-66138851 | MS.gene070073:CDS | 50.0% | |
| CCTCTCTTGTTCAGATCGAG+GGG | + | chr5.3:66138834-66138853 | MS.gene070073:CDS | 50.0% | |
| CTCTCTTGTTCAGATCGAGG+GGG | + | chr5.3:66138835-66138854 | MS.gene070073:CDS | 50.0% | |
| GTAGCCACTATCGCTGCATT+TGG | + | chr5.3:66138936-66138955 | MS.gene070073:CDS | 50.0% | |
| TACCCCAAATGCAGCGATAG+TGG | - | chr5.3:66138943-66138962 | None:intergenic | 50.0% | |
| TTTGTTTGTGCAGGGAGCCA+GGG | + | chr5.3:66139119-66139138 | MS.gene070073:intron | 50.0% | |
| ! | CTATCGCTGCATTTGGGGTA+AGG | + | chr5.3:66138943-66138962 | MS.gene070073:CDS | 50.0% |
| ! | GCATTTGGGGTAAGGTTACC+AGG | + | chr5.3:66138951-66138970 | MS.gene070073:CDS | 50.0% |
| ! | TATGTCAGGGGCACAGTTCT+TGG | + | chr5.3:66138215-66138234 | MS.gene070073:CDS | 50.0% |
| ACACCAAGGAGGAGGTTGCA+TGG | + | chr5.3:66138861-66138880 | MS.gene070073:CDS | 55.0% | |
| ACCACTGTTACCATGAGGCC+TGG | - | chr5.3:66138972-66138991 | None:intergenic | 55.0% | |
| ACCAGGCCTCATGGTAACAG+TGG | + | chr5.3:66138968-66138987 | MS.gene070073:CDS | 55.0% | |
| CCCCTCGATCTGAACAAGAG+AGG | - | chr5.3:66138837-66138856 | None:intergenic | 55.0% | |
| GACGTCAAGGAGAACGCATC+AGG | + | chr5.3:66138090-66138109 | MS.gene070073:CDS | 55.0% | |
| GAGGAGGTTGCATGGTATGC+TGG | + | chr5.3:66138869-66138888 | MS.gene070073:CDS | 55.0% | |
| GATCGAGGGGGTAAACACCA+AGG | + | chr5.3:66138847-66138866 | MS.gene070073:CDS | 55.0% | |
| GGTAAGGTTACCAGGCCTCA+TGG | + | chr5.3:66138959-66138978 | MS.gene070073:CDS | 55.0% | |
| CGAGGGGGTAAACACCAAGG+AGG | + | chr5.3:66138850-66138869 | MS.gene070073:CDS | 60.0% | |
| GGGGGTAAACACCAAGGAGG+AGG | + | chr5.3:66138853-66138872 | MS.gene070073:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 66138080 | 66139173 | 66138080 | ID=MS.gene070073 |
| chr5.3 | mRNA | 66138080 | 66139173 | 66138080 | ID=MS.gene070073.t1;Parent=MS.gene070073 |
| chr5.3 | exon | 66138080 | 66138111 | 66138080 | ID=MS.gene070073.t1.exon1;Parent=MS.gene070073.t1 |
| chr5.3 | CDS | 66138080 | 66138111 | 66138080 | ID=cds.MS.gene070073.t1;Parent=MS.gene070073.t1 |
| chr5.3 | exon | 66138211 | 66138246 | 66138211 | ID=MS.gene070073.t1.exon2;Parent=MS.gene070073.t1 |
| chr5.3 | CDS | 66138211 | 66138246 | 66138211 | ID=cds.MS.gene070073.t1;Parent=MS.gene070073.t1 |
| chr5.3 | exon | 66138808 | 66139036 | 66138808 | ID=MS.gene070073.t1.exon3;Parent=MS.gene070073.t1 |
| chr5.3 | CDS | 66138808 | 66139036 | 66138808 | ID=cds.MS.gene070073.t1;Parent=MS.gene070073.t1 |
| chr5.3 | exon | 66139132 | 66139173 | 66139132 | ID=MS.gene070073.t1.exon4;Parent=MS.gene070073.t1 |
| chr5.3 | CDS | 66139132 | 66139173 | 66139132 | ID=cds.MS.gene070073.t1;Parent=MS.gene070073.t1 |
| Gene Sequence |
| Protein sequence |
