AlfalfaGEDB | MS.gene020215

M. sativa cultivar XinJiangDaYe / MS.gene020215

KEGG: K14843
GO: GO:0005575 GO:0005730 GO:0008150 GO:0022613 GO:0042254 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0071840 GO:0110165
PFAM: PF06732.14 PF16589.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020215.t1	KEH18053.1	90.7	539	37	1	4	529	18	556	8.90E-278	965.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020215.t1	Q851S7	58.4	505	207	2	16	520	37	538	2.4e-170	600.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020215.t1	A0A072TMH4	90.7	539	37	1	4	529	18	556	6.4e-278	965.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene003490	MS.gene020215	PPI
MS.gene006800	MS.gene020215	PPI
MS.gene26979	MS.gene020215	PPI
MS.gene045547	MS.gene020215	PPI
MS.gene020215	MS.gene08488	PPI
MS.gene53345	MS.gene020215	PPI
MS.gene020215	MS.gene25010	PPI
MS.gene020215	MS.gene002204	PPI
MS.gene41329	MS.gene020215	PPI
MS.gene07704	MS.gene020215	PPI
MS.gene073150	MS.gene020215	PPI
MS.gene020215	MS.gene001917	PPI
MS.gene050985	MS.gene020215	PPI
MS.gene020215	MS.gene006661	PPI
MS.gene020215	MS.gene30895	PPI
MS.gene020215	MS.gene052717	PPI
MS.gene020215	MS.gene029880	PPI
MS.gene020215	MS.gene031484	PPI
MS.gene92142	MS.gene020215	PPI
MS.gene020215	MS.gene014499	PPI
MS.gene020215	MS.gene044819	PPI
MS.gene89671	MS.gene020215	PPI
MS.gene068781	MS.gene020215	PPI
MS.gene073324	MS.gene020215	PPI
MS.gene059726	MS.gene020215	PPI
MS.gene051904	MS.gene020215	PPI
MS.gene98461	MS.gene020215	PPI
MS.gene07808	MS.gene020215	PPI
MS.gene60693	MS.gene020215	PPI
MS.gene028002	MS.gene020215	PPI
MS.gene017623	MS.gene020215	PPI
MS.gene020215	MS.gene064682	PPI
MS.gene020215	MS.gene038800	PPI
MS.gene012331	MS.gene020215	PPI
MS.gene020215	MS.gene041557	PPI
MS.gene020215	MS.gene034157	PPI
MS.gene23058	MS.gene020215	PPI
MS.gene020215	MS.gene41916	PPI
MS.gene81068	MS.gene020215	PPI
MS.gene020215	MS.gene034931	PPI
MS.gene031378	MS.gene020215	PPI
MS.gene063385	MS.gene020215	PPI
MS.gene52150	MS.gene020215	PPI
MS.gene04336	MS.gene020215	PPI
MS.gene044537	MS.gene020215	PPI
MS.gene020215	MS.gene69196	PPI
MS.gene058018	MS.gene020215	PPI
MS.gene020215	MS.gene30325	PPI
MS.gene020215	MS.gene86495	PPI
MS.gene020215	MS.gene071916	PPI
MS.gene020215	MS.gene89319	PPI
MS.gene020215	MS.gene89037	PPI
MS.gene99744	MS.gene020215	PPI
MS.gene96312	MS.gene020215	PPI
MS.gene000604	MS.gene020215	PPI
MS.gene08434	MS.gene020215	PPI
MS.gene53720	MS.gene020215	PPI
MS.gene045180	MS.gene020215	PPI
MS.gene020215	MS.gene61130	PPI
MS.gene020215	MS.gene44231	PPI
MS.gene047125	MS.gene020215	PPI
MS.gene020215	MS.gene46475	PPI
MS.gene020215	MS.gene98681	PPI
MS.gene020215	MS.gene024348	PPI
MS.gene55098	MS.gene020215	PPI
MS.gene020215	MS.gene060923	PPI
MS.gene020215	MS.gene43350	PPI
MS.gene029242	MS.gene020215	PPI
MS.gene020215	MS.gene52105	PPI
MS.gene53546	MS.gene020215	PPI
MS.gene020215	MS.gene001986	PPI
MS.gene020215	MS.gene031657	PPI
MS.gene065652	MS.gene020215	PPI
MS.gene033382	MS.gene020215	PPI
MS.gene04159	MS.gene020215	PPI
MS.gene020215	MS.gene053467	PPI
MS.gene020215	MS.gene061428	PPI
MS.gene01635	MS.gene020215	PPI
MS.gene007067	MS.gene020215	PPI
MS.gene020215	MS.gene81001	PPI
MS.gene006154	MS.gene020215	PPI
MS.gene020215	MS.gene038784	PPI
MS.gene72708	MS.gene020215	PPI
MS.gene09038	MS.gene020215	PPI
MS.gene020215	MS.gene002126	PPI
MS.gene020215	MS.gene071987	PPI
MS.gene070298	MS.gene020215	PPI
MS.gene011268	MS.gene020215	PPI
MS.gene68979	MS.gene020215	PPI
MS.gene073795	MS.gene020215	PPI
MS.gene021058	MS.gene020215	PPI
MS.gene81498	MS.gene020215	PPI
MS.gene020215	MS.gene45470	PPI
MS.gene020215	MS.gene031660	PPI
MS.gene020215	MS.gene060638	PPI
MS.gene053148	MS.gene020215	PPI
MS.gene032998	MS.gene020215	PPI
MS.gene80570	MS.gene020215	PPI
MS.gene020215	MS.gene56166	PPI
MS.gene020215	MS.gene77667	PPI
MS.gene020215	MS.gene88918	PPI
MS.gene020215	MS.gene99779	PPI
MS.gene020215	MS.gene072064	PPI
MS.gene020215	MS.gene75598	PPI
MS.gene56696	MS.gene020215	PPI
MS.gene020215	MS.gene65051	PPI
MS.gene29581	MS.gene020215	PPI
MS.gene039474	MS.gene020215	PPI
MS.gene020215	MS.gene002038	PPI
MS.gene064180	MS.gene020215	PPI
MS.gene88081	MS.gene020215	PPI
MS.gene035194	MS.gene020215	PPI
MS.gene020215	MS.gene23276	PPI
MS.gene020215	MS.gene011421	PPI
MS.gene037902	MS.gene020215	PPI
MS.gene07810	MS.gene020215	PPI
MS.gene70551	MS.gene020215	PPI
MS.gene91244	MS.gene020215	PPI
MS.gene94588	MS.gene020215	PPI
MS.gene83200	MS.gene020215	PPI
MS.gene020215	MS.gene90945	PPI
MS.gene011230	MS.gene020215	PPI
MS.gene065189	MS.gene020215	PPI
MS.gene020215	MS.gene054186	PPI
MS.gene020215	MS.gene45902	PPI
MS.gene020215	MS.gene69611	PPI
MS.gene020215	MS.gene057738	PPI
MS.gene020215	MS.gene066830	PPI
MS.gene46104	MS.gene020215	PPI
MS.gene46105	MS.gene020215	PPI
MS.gene020215	MS.gene047206	PPI
MS.gene91058	MS.gene020215	PPI
MS.gene020215	MS.gene057307	PPI
MS.gene020215	MS.gene80639	PPI
MS.gene020215	MS.gene018651	PPI
MS.gene061835	MS.gene020215	PPI
MS.gene92970	MS.gene020215	PPI
MS.gene020215	MS.gene012126	PPI
MS.gene073911	MS.gene020215	PPI
MS.gene54912	MS.gene020215	PPI
MS.gene012333	MS.gene020215	PPI
MS.gene070073	MS.gene020215	PPI
MS.gene045265	MS.gene020215	PPI
MS.gene020215	MS.gene044498	PPI
MS.gene059846	MS.gene020215	PPI
MS.gene051451	MS.gene020215	PPI
MS.gene047421	MS.gene020215	PPI
MS.gene020215	MS.gene003735	PPI
MS.gene05689	MS.gene020215	PPI
MS.gene82489	MS.gene020215	PPI
MS.gene67365	MS.gene020215	PPI
MS.gene020215	MS.gene035331	PPI
MS.gene036487	MS.gene020215	PPI
MS.gene020215	MS.gene30933	PPI
MS.gene020215	MS.gene033017	PPI
MS.gene056257	MS.gene020215	PPI
MS.gene020215	MS.gene24260	PPI
MS.gene018599	MS.gene020215	PPI
MS.gene020215	MS.gene010453	PPI
MS.gene020215	MS.gene01172	PPI
MS.gene053230	MS.gene020215	PPI
MS.gene020215	MS.gene00612	PPI
MS.gene047734	MS.gene020215	PPI
MS.gene020215	MS.gene09204	PPI
MS.gene020215	MS.gene034607	PPI
MS.gene020215	MS.gene054580	PPI
MS.gene020215	MS.gene96780	PPI
MS.gene007066	MS.gene020215	PPI
MS.gene020215	MS.gene045818	PPI
MS.gene020215	MS.gene42882	PPI
MS.gene059880	MS.gene020215	PPI
MS.gene020215	MS.gene59609	PPI
MS.gene004091	MS.gene020215	PPI
MS.gene23437	MS.gene020215	PPI
MS.gene069038	MS.gene020215	PPI
MS.gene040271	MS.gene020215	PPI
MS.gene020215	MS.gene031692	PPI
MS.gene68238	MS.gene020215	PPI
MS.gene020215	MS.gene31177	PPI
MS.gene95758	MS.gene020215	PPI
MS.gene020215	MS.gene046040	PPI
MS.gene051903	MS.gene020215	PPI
MS.gene020215	MS.gene23059	PPI
MS.gene055201	MS.gene020215	PPI
MS.gene31515	MS.gene020215	PPI
MS.gene86675	MS.gene020215	PPI
MS.gene020215	MS.gene006618	PPI
MS.gene059878	MS.gene020215	PPI
MS.gene014498	MS.gene020215	PPI
MS.gene80786	MS.gene020215	PPI
MS.gene95644	MS.gene020215	PPI
MS.gene037074	MS.gene020215	PPI
MS.gene020215	MS.gene000480	PPI
MS.gene059422	MS.gene020215	PPI
MS.gene020215	MS.gene003492	PPI
MS.gene020215	MS.gene067072	PPI
MS.gene020215	MS.gene029415	PPI
MS.gene020215	MS.gene79721	PPI
MS.gene020215	MS.gene040269	PPI
MS.gene039132	MS.gene020215	PPI
MS.gene020215	MS.gene57660	PPI
MS.gene67544	MS.gene020215	PPI
MS.gene020215	MS.gene90360	PPI
MS.gene020215	MS.gene66119	PPI
MS.gene037324	MS.gene020215	PPI
MS.gene020215	MS.gene73825	PPI
MS.gene034156	MS.gene020215	PPI
MS.gene020215	MS.gene75537	PPI
MS.gene032493	MS.gene020215	PPI
MS.gene040601	MS.gene020215	PPI
MS.gene045580	MS.gene020215	PPI
MS.gene020215	MS.gene000625	PPI
MS.gene020215	MS.gene40564	PPI
MS.gene020215	MS.gene065146	PPI
MS.gene72993	MS.gene020215	PPI
MS.gene59228	MS.gene020215	PPI
MS.gene040272	MS.gene020215	PPI
MS.gene020215	MS.gene42324	PPI
MS.gene066811	MS.gene020215	PPI
MS.gene62799	MS.gene020215	PPI
MS.gene020215	MS.gene98171	PPI
MS.gene020215	MS.gene47347	PPI
MS.gene020215	MS.gene064459	PPI
MS.gene59319	MS.gene020215	PPI
MS.gene020215	MS.gene031406	PPI
MS.gene020215	MS.gene39195	PPI
MS.gene028609	MS.gene020215	PPI
MS.gene07852	MS.gene020215	PPI
MS.gene003435	MS.gene020215	PPI
MS.gene034255	MS.gene020215	PPI
MS.gene020215	MS.gene37379	PPI
MS.gene020215	MS.gene77392	PPI
MS.gene020215	MS.gene45344	PPI
MS.gene029547	MS.gene020215	PPI
MS.gene020215	MS.gene56525	PPI
MS.gene020215	MS.gene96949	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020215.t1	MTR_8g010330	91.280	539	34	1	4	529	18	556	0.0	1019
MS.gene020215.t1	MTR_7g100040	67.832	572	126	5	16	529	38	609	0.0	781
MS.gene020215.t1	MTR_7g100040	65.021	466	105	6	122	529	22	487	0.0	597
MS.gene020215.t1	MTR_6g022610	67.778	180	16	2	116	295	1	138	3.69e-73	229
MS.gene020215.t1	MTR_6g022610	78.261	92	16	1	185	276	1	88	1.94e-42	147
MS.gene020215.t1	MTR_6g022570	59.434	106	29	1	409	500	30	135	8.31e-34	125
MS.gene020215.t1	MTR_6g022550	71.622	74	17	1	23	96	26	95	4.22e-27	108
MS.gene020215.t1	MTR_6g022550	66.234	77	18	1	413	489	87	155	1.17e-24	101
MS.gene020215.t1	MTR_6g022580	50.962	104	29	2	420	523	24	105	4.95e-20	85.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene020215.t1	AT5G14520	54.383	559	190	4	20	525	41	587	0.0	595

Find 109 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGCGGAAGAATTTCCTTTC+TGG	0.208018	8.4:-78215145	None:intergenic
CGAATAATCATGTCTATCTT+TGG	0.249220	8.4:-78212791	None:intergenic
ATCATGTCTATCTTTGGTTT+TGG	0.308718	8.4:-78212785	None:intergenic
TCTTCACCACGAACCTTTGC+TGG	0.325259	8.4:+78212667	MS.gene020215:CDS
TTTGCCAACAGAAAGTTATA+TGG	0.327582	8.4:+78214618	MS.gene020215:CDS
AACTCCTCATTTACTACAAC+AGG	0.333847	8.4:+78213367	MS.gene020215:CDS
CCAAGTGCATCCACAAATCT+TGG	0.344978	8.4:-78212910	None:intergenic
CAAGCAGAGTCCAAGCAAAA+AGG	0.356666	8.4:+78213846	MS.gene020215:CDS
TGAAAAGAGGTAGACTGATT+TGG	0.375506	8.4:-78212402	None:intergenic
CCATTTCATGGGAGGGAAAG+GGG	0.378833	8.4:+78214380	MS.gene020215:CDS
CTTATGATGCCTTGTAGAAA+AGG	0.384660	8.4:+78215318	MS.gene020215:CDS
ACCCCGACAGCCTAAGAAAA+AGG	0.389796	8.4:+78212598	MS.gene020215:CDS
TGCACTGATGCACCCGGTTA+AGG	0.396358	8.4:+78213944	MS.gene020215:CDS
TCACCTGCTAAGTCCTTAAC+CGG	0.396645	8.4:-78213957	None:intergenic
TGTGGATGCACTTGGAGAAT+TGG	0.399033	8.4:+78212918	MS.gene020215:CDS
CAAAGATAGACATGATTATT+CGG	0.399051	8.4:+78212792	MS.gene020215:CDS
CCCCTTTCCCTCCCATGAAA+TGG	0.401718	8.4:-78214380	None:intergenic
CCTCTATCATTCTATAAACT+TGG	0.401850	8.4:+78213599	MS.gene020215:CDS
AATTGCCACAGCCTCTTCTT+CGG	0.420224	8.4:+78213562	MS.gene020215:intron
TTGATAGTCTTTGCATAGTC+GGG	0.423497	8.4:-78215105	None:intergenic
ACCATTTCATGGGAGGGAAA+GGG	0.429224	8.4:+78214379	MS.gene020215:CDS
TGACCAGAGCATTACTCATC+AGG	0.431073	8.4:+78214420	MS.gene020215:CDS
CCAAGATTTGTGGATGCACT+TGG	0.444772	8.4:+78212910	MS.gene020215:CDS
CATTTGTGTAGATTGTTGAC+AGG	0.449441	8.4:+78214515	MS.gene020215:intron
CTCTGGTCAGATTCATCAAA+AGG	0.450256	8.4:-78214406	None:intergenic
CGGTTGGAAGCTTTCGCAGC+AGG	0.453005	8.4:+78213645	MS.gene020215:CDS
CACCATTTCATGGGAGGGAA+AGG	0.455706	8.4:+78214378	MS.gene020215:CDS
CTTGCAAGCTGCTGCCAGAA+AGG	0.459351	8.4:+78215131	MS.gene020215:CDS
TCTTAAATTCTTCTTGAGTA+GGG	0.459842	8.4:+78214028	MS.gene020215:CDS
TCCAGAGGGTTTGCTCGCAT+TGG	0.462811	8.4:-78213787	None:intergenic
GTAGCCTGTTTGCAAGTTCG+TGG	0.464814	8.4:-78212751	None:intergenic
ATTCTTCCGCTTCCTAGTGT+TGG	0.466110	8.4:+78215156	MS.gene020215:CDS
ATCTTAAATTCTTCTTGAGT+AGG	0.472930	8.4:+78214027	MS.gene020215:CDS
TTTGTTAACTATGACAAAGA+AGG	0.474242	8.4:+78215075	MS.gene020215:CDS
TTTGTGCAGGCTGAGGTTGA+GGG	0.480167	8.4:+78213326	MS.gene020215:intron
AAAACCACGAACTTGCAAAC+AGG	0.482442	8.4:+78212747	MS.gene020215:CDS
TACAAATAATACCTGAAAAG+AGG	0.485388	8.4:-78212415	None:intergenic
ACGAACTTGCAAACAGGCTA+CGG	0.491947	8.4:+78212753	MS.gene020215:CDS
GCCAATGCGAGCAAACCCTC+TGG	0.496559	8.4:+78213786	MS.gene020215:CDS
CAGGTTCATTAGAAAGAAGT+TGG	0.499834	8.4:-78213920	None:intergenic
TTATAGGTACCCAAGATTTG+TGG	0.501589	8.4:+78212900	MS.gene020215:intron
TCTTTGTCATAGTTAACAAA+AGG	0.508753	8.4:-78215072	None:intergenic
CCAAGTTTATAGAATGATAG+AGG	0.510142	8.4:-78213599	None:intergenic
TCTAATTACTCAGGTACAAA+GGG	0.510871	8.4:+78214321	MS.gene020215:intron
TGCAGAAAATTTAGCATGGT+AGG	0.520334	8.4:-78213413	None:intergenic
ATCCTCCCAGGCTTGATACT+CGG	0.520338	8.4:+78213625	MS.gene020215:CDS
TAGACATGATTATTCGGCAG+AGG	0.521068	8.4:+78212798	MS.gene020215:CDS
GTTCTTCCAGCAAAGGTTCG+TGG	0.522161	8.4:-78212673	None:intergenic
CAAGTGCATCCACAAATCTT+GGG	0.522176	8.4:-78212909	None:intergenic
GTCTAATTACTCAGGTACAA+AGG	0.527454	8.4:+78214320	MS.gene020215:intron
ACAGGGATGCGCAAGGCCAT+AGG	0.528933	8.4:+78214533	MS.gene020215:CDS
GAAATGGTGCCACCAAAAGC+CGG	0.530863	8.4:-78214364	None:intergenic
CCCACCATATAACTTTCTGT+TGG	0.531260	8.4:-78214622	None:intergenic
TTTGATAGTCTTTGCATAGT+CGG	0.533193	8.4:-78215106	None:intergenic
GCCAACAGAAAGTTATATGG+TGG	0.533732	8.4:+78214621	MS.gene020215:CDS
CTTAGCAGGTGAAGATGAAG+AGG	0.534642	8.4:+78213968	MS.gene020215:CDS
GTTCTATGTTCTTCCAGCAA+AGG	0.536261	8.4:-78212680	None:intergenic
CAACTTCTTTCTAATGAACC+TGG	0.539088	8.4:+78213921	MS.gene020215:CDS
AATGAAATTAGACGAAGCTA+AGG	0.539472	8.4:+78215571	MS.gene020215:CDS
AAAAGAAACATCTGCTGTTG+TGG	0.540444	8.4:+78215236	MS.gene020215:intron
TTGCTAGATTGGCACATGAA+TGG	0.542766	8.4:+78213160	MS.gene020215:intron
AAAACATATGTATCGGTGAA+AGG	0.547089	8.4:+78213216	MS.gene020215:CDS
GGTGGCACCATTTCATGGGA+GGG	0.549937	8.4:+78214373	MS.gene020215:CDS
AAACCTGATGAGTAATGCTC+TGG	0.551841	8.4:-78214423	None:intergenic
ACCGGGTGCATCAGTGCACC+AGG	0.551844	8.4:-78213939	None:intergenic
CACCCGGTTAAGGACTTAGC+AGG	0.554532	8.4:+78213954	MS.gene020215:CDS
TAGACGAAGCTAAGGGTCAA+AGG	0.556106	8.4:+78215579	MS.gene020215:CDS
ATTCTCTAGATAGAAACCTA+TGG	0.559229	8.4:-78214549	None:intergenic
TTCCAACCGAGTATCAAGCC+TGG	0.561392	8.4:-78213631	None:intergenic
ATGATGCCTTGTAGAAAAGG+AGG	0.561467	8.4:+78215321	MS.gene020215:CDS
TGATGCCTTGTAGAAAAGGA+GGG	0.562121	8.4:+78215322	MS.gene020215:CDS
GTTGAGGAAAACATATGTAT+CGG	0.567474	8.4:+78213209	MS.gene020215:CDS
CAGAAAGTTATATGGTGGGA+AGG	0.567604	8.4:+78214626	MS.gene020215:CDS
TCCCAGGCTTGATACTCGGT+TGG	0.569177	8.4:+78213629	MS.gene020215:CDS
TCCAACCGAGTATCAAGCCT+GGG	0.572418	8.4:-78213630	None:intergenic
CGAACTTGCAAACAGGCTAC+GGG	0.574429	8.4:+78212754	MS.gene020215:CDS
TGGTGGCACCATTTCATGGG+AGG	0.574788	8.4:+78214372	MS.gene020215:CDS
ATGCTGCAAAATTTGTCACT+AGG	0.575876	8.4:+78212359	MS.gene020215:CDS
ACAACCTGTTGTAGTAAATG+AGG	0.582229	8.4:-78213371	None:intergenic
CATTTCATGGGAGGGAAAGG+GGG	0.584771	8.4:+78214381	MS.gene020215:CDS
TCTGCTGTTGTGGTACAGAC+TGG	0.585277	8.4:+78215246	MS.gene020215:CDS
AAAGGTGACAAGTGAGGCGG+AGG	0.592412	8.4:-78215054	None:intergenic
AAATTCACAAACCGAAGAAG+AGG	0.592721	8.4:-78213573	None:intergenic
CCAACAGAAAGTTATATGGT+GGG	0.593527	8.4:+78214622	MS.gene020215:CDS
GCTTCAAGTATCAGTCCAGA+GGG	0.593688	8.4:-78213801	None:intergenic
TAAATTCTTCTTGAGTAGGG+AGG	0.600886	8.4:+78214031	MS.gene020215:CDS
GTTCATTAGAAAGAAGTTGG+TGG	0.606617	8.4:-78213917	None:intergenic
GAGAATATGTTCAACCTCAG+TGG	0.608365	8.4:+78214566	MS.gene020215:CDS
TAAACTTGGAGTATCCTCCC+AGG	0.613538	8.4:+78213613	MS.gene020215:CDS
TATCTCGTACTCACAAGTTG+AGG	0.614176	8.4:+78213193	MS.gene020215:CDS
AGCTTCAAGTATCAGTCCAG+AGG	0.619437	8.4:-78213802	None:intergenic
ACTGGAGAATCAACAACGAA+TGG	0.632388	8.4:+78215264	MS.gene020215:CDS
ATTTGTGTAGATTGTTGACA+GGG	0.635765	8.4:+78214516	MS.gene020215:intron
CACCTGCTAAGTCCTTAACC+GGG	0.639120	8.4:-78213956	None:intergenic
GGTGAAAGGCATATACTACC+AGG	0.639619	8.4:+78213230	MS.gene020215:CDS
ATGAAATTAGACGAAGCTAA+GGG	0.640863	8.4:+78215572	MS.gene020215:CDS
AAAAGGTCAAGGGAACTCAT+CGG	0.645163	8.4:+78212615	MS.gene020215:CDS
ATTGTTGACAGGGATGCGCA+AGG	0.648110	8.4:+78214526	MS.gene020215:CDS
ACCTGGTGCACTGATGCACC+CGG	0.657335	8.4:+78213938	MS.gene020215:CDS
AGCTTGTACACAAATGCCGA+AGG	0.666012	8.4:+78213010	MS.gene020215:CDS
AAATTGCAGAAAATTTAGCA+TGG	0.667615	8.4:-78213417	None:intergenic
TTAACAAAAGGTGACAAGTG+AGG	0.668766	8.4:-78215060	None:intergenic
ATTGGACGACTGTCTTACAA+TGG	0.686764	8.4:+78212936	MS.gene020215:CDS
AACCGAGTATCAAGCCTGGG+AGG	0.694808	8.4:-78213627	None:intergenic
AGTTTATAGAATGATAGAGG+CGG	0.695179	8.4:-78213596	None:intergenic
AGACTGATCGCATCAAACAG+CGG	0.702142	8.4:+78215546	MS.gene020215:CDS
TTGAGGGTCAGACAATAACA+TGG	0.704070	8.4:+78213342	MS.gene020215:CDS
ACAAAAGGTGACAAGTGAGG+CGG	0.709902	8.4:-78215057	None:intergenic
ACACAATCAAATATCCACTG+AGG	0.780940	8.4:-78214580	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAAATAAATTAAGAAGAT+AGG	-	chr8.4:78213127-78213146	None:intergenic	10.0%
!!	CATATTAATGTTTATATTTA+GGG	+	chr8.4:78213454-78213473	MS.gene020215:intron	10.0%
!!	TCATATTAATGTTTATATTT+AGG	+	chr8.4:78213453-78213472	MS.gene020215:intron	10.0%
!!	TATTAAAAAACTACCTTATA+TGG	+	chr8.4:78214829-78214848	MS.gene020215:intron	15.0%
!!	TGAAATGAATCAATAATATA+TGG	+	chr8.4:78214177-78214196	MS.gene020215:intron	15.0%
!!!	AACATTTTAAGTTTTTAACT+AGG	-	chr8.4:78215016-78215035	None:intergenic	15.0%
!!!	ACATTTTAAGTTTTTAACTA+GGG	-	chr8.4:78215015-78215034	None:intergenic	15.0%
!!	AAAAAATTGTACATGTCATA+TGG	+	chr8.4:78213285-78213304	MS.gene020215:intron	20.0%
!!	AAAATAATAAGCACAGAAAA+TGG	-	chr8.4:78214662-78214681	None:intergenic	20.0%
!!	GATATATAATAGAGTATATG+CGG	-	chr8.4:78213714-78213733	None:intergenic	20.0%
!!!	AAAATGTTTCTTTTGTTAAC+AGG	+	chr8.4:78215028-78215047	MS.gene020215:intron	20.0%
!!!	AAATTTTCTGCAATTTTACA+AGG	+	chr8.4:78213424-78213443	MS.gene020215:CDS	20.0%
!!!	ATCATTACTTTTTGTTATTC+CGG	+	chr8.4:78214345-78214364	MS.gene020215:CDS	20.0%
!!!	ATTTTCTGTGCTTATTATTT+TGG	+	chr8.4:78214661-78214680	MS.gene020215:intron	20.0%
!!!	GTCTAATTTTCTAAAACTAT+TGG	-	chr8.4:78214869-78214888	None:intergenic	20.0%
!!!	TATTGTTTCTGTGAATAATT+GGG	+	chr8.4:78214096-78214115	MS.gene020215:intron	20.0%
!!!	TTATTATTTTGGGTTCATTA+GGG	+	chr8.4:78214672-78214691	MS.gene020215:intron	20.0%
!!!	TTGATTTTCCTATGTATTAT+AGG	+	chr8.4:78212884-78212903	MS.gene020215:intron	20.0%
!!!	TTTTCTGTGCTTATTATTTT+GGG	+	chr8.4:78214662-78214681	MS.gene020215:intron	20.0%
!	AAATTGCAGAAAATTTAGCA+TGG	-	chr8.4:78213420-78213439	None:intergenic	25.0%
!	ATATTAGGAAACTAAGCATT+TGG	+	chr8.4:78212564-78212583	MS.gene020215:intron	25.0%
!	ATCTTAAATTCTTCTTGAGT+AGG	+	chr8.4:78214027-78214046	MS.gene020215:CDS	25.0%
!	ATTAAAAAAGTTTACCGTCA+TGG	-	chr8.4:78215358-78215377	None:intergenic	25.0%
!	CAAAGATAGACATGATTATT+CGG	+	chr8.4:78212792-78212811	MS.gene020215:CDS	25.0%
!	CATAGGAAAATCAAATAAAC+TGG	-	chr8.4:78212878-78212897	None:intergenic	25.0%
!	TAAAAAACTACCTTATATGG+TGG	+	chr8.4:78214832-78214851	MS.gene020215:intron	25.0%
!	TACAAATAATACCTGAAAAG+AGG	-	chr8.4:78212418-78212437	None:intergenic	25.0%
!	TCAAATTTGCACTTTCTTAA+AGG	-	chr8.4:78213092-78213111	None:intergenic	25.0%
!	TCTTAAATTCTTCTTGAGTA+GGG	+	chr8.4:78214028-78214047	MS.gene020215:CDS	25.0%
!	TCTTTGTCATAGTTAACAAA+AGG	-	chr8.4:78215075-78215094	None:intergenic	25.0%
!	TTCCAAGTATTATATACCTT+CGG	-	chr8.4:78213029-78213048	None:intergenic	25.0%
!	TTTAGAAAATTAGACCATCT+TGG	+	chr8.4:78214874-78214893	MS.gene020215:intron	25.0%
!	TTTGTTAACTATGACAAAGA+AGG	+	chr8.4:78215075-78215094	MS.gene020215:CDS	25.0%
!!	ATGGGTTAAGTATTTACAAT+TGG	+	chr8.4:78215442-78215461	MS.gene020215:intron	25.0%
!!	CAAATTAGTGTTTTCTTCAA+GGG	+	chr8.4:78214747-78214766	MS.gene020215:intron	25.0%
!!!	CTTATTATTTTGGGTTCATT+AGG	+	chr8.4:78214671-78214690	MS.gene020215:intron	25.0%
!!!	GATTGATTATATTTTTGTGC+AGG	+	chr8.4:78213313-78213332	MS.gene020215:intron	25.0%
!!!	GTATTGTTTCTGTGAATAAT+TGG	+	chr8.4:78214095-78214114	MS.gene020215:intron	25.0%
	AAACATGAGCACATAGAATA+TGG	-	chr8.4:78214932-78214951	None:intergenic	30.0%
	AACATGAGCACATAGAATAT+GGG	-	chr8.4:78214931-78214950	None:intergenic	30.0%
	AATCTACACAAATGAAAGCA+AGG	-	chr8.4:78214509-78214528	None:intergenic	30.0%
	AATGAAATTAGACGAAGCTA+AGG	+	chr8.4:78215571-78215590	MS.gene020215:CDS	30.0%
	AGTTTATAGAATGATAGAGG+CGG	-	chr8.4:78213599-78213618	None:intergenic	30.0%
	ATCTACACAAATGAAAGCAA+GGG	-	chr8.4:78214508-78214527	None:intergenic	30.0%
	ATGAAATTAGACGAAGCTAA+GGG	+	chr8.4:78215572-78215591	MS.gene020215:CDS	30.0%
	ATTCTCTAGATAGAAACCTA+TGG	-	chr8.4:78214552-78214571	None:intergenic	30.0%
	ATTTGTGTAGATTGTTGACA+GGG	+	chr8.4:78214516-78214535	MS.gene020215:intron	30.0%
	CCAAGTTTATAGAATGATAG+AGG	-	chr8.4:78213602-78213621	None:intergenic	30.0%
	CCTCTATCATTCTATAAACT+TGG	+	chr8.4:78213599-78213618	MS.gene020215:CDS	30.0%
	CGAATAATCATGTCTATCTT+TGG	-	chr8.4:78212794-78212813	None:intergenic	30.0%
	CTCAATTTATATCTTGTAGC+TGG	+	chr8.4:78212841-78212860	MS.gene020215:intron	30.0%
	GGCAATTCATTATGAAGTTA+AGG	-	chr8.4:78213549-78213568	None:intergenic	30.0%
	GTTGAGGAAAACATATGTAT+CGG	+	chr8.4:78213209-78213228	MS.gene020215:CDS	30.0%
	TACTACTTCGATTCTTACAT+TGG	+	chr8.4:78212532-78212551	MS.gene020215:intron	30.0%
	TAGCAAGATGAAAAGAAGAA+AGG	-	chr8.4:78214811-78214830	None:intergenic	30.0%
	TCTAATTACTCAGGTACAAA+GGG	+	chr8.4:78214321-78214340	MS.gene020215:intron	30.0%
	TGAAAAGAAGAAAGGATATG+TGG	-	chr8.4:78214803-78214822	None:intergenic	30.0%
	TGTGAATAATTGGGAAATCA+AGG	+	chr8.4:78214105-78214124	MS.gene020215:intron	30.0%
	TTTGCCAACAGAAAGTTATA+TGG	+	chr8.4:78214618-78214637	MS.gene020215:CDS	30.0%
!	AAAACATATGTATCGGTGAA+AGG	+	chr8.4:78213216-78213235	MS.gene020215:CDS	30.0%
!	GCAAATTAGTGTTTTCTTCA+AGG	+	chr8.4:78214746-78214765	MS.gene020215:intron	30.0%
!	TACCATTTTCTGAACTTAGA+TGG	+	chr8.4:78213668-78213687	MS.gene020215:intron	30.0%
!	TTTCTGCAATTTTACAAGGT+CGG	+	chr8.4:78213428-78213447	MS.gene020215:intron	30.0%
!	TTTGATAGTCTTTGCATAGT+CGG	-	chr8.4:78215109-78215128	None:intergenic	30.0%
!	TTTGTTGTAGCAATCTTTGT+AGG	+	chr8.4:78214701-78214720	MS.gene020215:intron	30.0%
!!	ATCATGTCTATCTTTGGTTT+TGG	-	chr8.4:78212788-78212807	None:intergenic	30.0%
!!!	CATTGGCTTTTGCAAATATT+AGG	+	chr8.4:78212549-78212568	MS.gene020215:intron	30.0%
	AAAAGAAACATCTGCTGTTG+TGG	+	chr8.4:78215236-78215255	MS.gene020215:intron	35.0%
	AAATTCACAAACCGAAGAAG+AGG	-	chr8.4:78213576-78213595	None:intergenic	35.0%
	AACTCCTCATTTACTACAAC+AGG	+	chr8.4:78213367-78213386	MS.gene020215:CDS	35.0%
	AAGCAACTCAAGTAAGCATA+AGG	-	chr8.4:78215383-78215402	None:intergenic	35.0%
	AATGGTTGCAAAGTGATTGA+TGG	+	chr8.4:78215423-78215442	MS.gene020215:intron	35.0%
	ACAACCTGTTGTAGTAAATG+AGG	-	chr8.4:78213374-78213393	None:intergenic	35.0%
	ACACAATCAAATATCCACTG+AGG	-	chr8.4:78214583-78214602	None:intergenic	35.0%
	AGGAAACTAAGCATTTGGAA+AGG	+	chr8.4:78212569-78212588	MS.gene020215:intron	35.0%
	ATACTACCAGGTATCAATTG+TGG	+	chr8.4:78213242-78213261	MS.gene020215:intron	35.0%
	ATATAGAAAGCGAGTCTATG+TGG	+	chr8.4:78214974-78214993	MS.gene020215:intron	35.0%
	ATGCTGCAAAATTTGTCACT+AGG	+	chr8.4:78212359-78212378	MS.gene020215:CDS	35.0%
	ATGGTTGCAAAGTGATTGAT+GGG	+	chr8.4:78215424-78215443	MS.gene020215:intron	35.0%
	CAACTTCTTTCTAATGAACC+TGG	+	chr8.4:78213921-78213940	MS.gene020215:CDS	35.0%
	CAGGTTCATTAGAAAGAAGT+TGG	-	chr8.4:78213923-78213942	None:intergenic	35.0%
	CATTTGTGTAGATTGTTGAC+AGG	+	chr8.4:78214515-78214534	MS.gene020215:intron	35.0%
	CCAACAGAAAGTTATATGGT+GGG	+	chr8.4:78214622-78214641	MS.gene020215:CDS	35.0%
	CTTATGATGCCTTGTAGAAA+AGG	+	chr8.4:78215318-78215337	MS.gene020215:CDS	35.0%
	CTTGGGTACCTATAATACAT+AGG	-	chr8.4:78212895-78212914	None:intergenic	35.0%
	GTCCATCTAAGTTCAGAAAA+TGG	-	chr8.4:78213673-78213692	None:intergenic	35.0%
	GTCTAATTACTCAGGTACAA+AGG	+	chr8.4:78214320-78214339	MS.gene020215:intron	35.0%
	GTTCATTAGAAAGAAGTTGG+TGG	-	chr8.4:78213920-78213939	None:intergenic	35.0%
	TAAATTCTTCTTGAGTAGGG+AGG	+	chr8.4:78214031-78214050	MS.gene020215:CDS	35.0%
	TGAAAAGAGGTAGACTGATT+TGG	-	chr8.4:78212405-78212424	None:intergenic	35.0%
	TGCAGAAAATTTAGCATGGT+AGG	-	chr8.4:78213416-78213435	None:intergenic	35.0%
	TTAACAAAAGGTGACAAGTG+AGG	-	chr8.4:78215063-78215082	None:intergenic	35.0%
	TTATAGGTACCCAAGATTTG+TGG	+	chr8.4:78212900-78212919	MS.gene020215:intron	35.0%
	TTCTTTGTCCTCTGCATAAA+TGG	+	chr8.4:78215405-78215424	MS.gene020215:intron	35.0%
	TTGATCAAGACCACCATATA+AGG	-	chr8.4:78214845-78214864	None:intergenic	35.0%
!	AAACAGTGCAAGTATTGTCA+AGG	-	chr8.4:78214298-78214317	None:intergenic	35.0%
!	AAATCAGTCTACCTCTTTTC+AGG	+	chr8.4:78212404-78212423	MS.gene020215:CDS	35.0%
!	GAATCTTCTTTTCCAACACT+AGG	-	chr8.4:78215171-78215190	None:intergenic	35.0%
!	TAAAACCCTCCTTTTCTACA+AGG	-	chr8.4:78215330-78215349	None:intergenic	35.0%
!	TGACAATACTTGCACTGTTT+TGG	+	chr8.4:78214298-78214317	MS.gene020215:intron	35.0%
!	TGCCGAAGGTATATAATACT+TGG	+	chr8.4:78213024-78213043	MS.gene020215:intron	35.0%
!	TTGATAGTCTTTGCATAGTC+GGG	-	chr8.4:78215108-78215127	None:intergenic	35.0%
!!	TTATATTTTTGTGCAGGCTG+AGG	+	chr8.4:78213319-78213338	MS.gene020215:intron	35.0%
!!!	CCTCGGCTTTCTTTATTTTT+CGG	-	chr8.4:78212721-78212740	None:intergenic	35.0%
!!!	CTGTTTTGGTCTAATTACTC+AGG	+	chr8.4:78214312-78214331	MS.gene020215:intron	35.0%
!!!	CTTTTTGTTATTCCGGCTTT+TGG	+	chr8.4:78214352-78214371	MS.gene020215:CDS	35.0%
	AAAACCACGAACTTGCAAAC+AGG	+	chr8.4:78212747-78212766	MS.gene020215:CDS	40.0%
	AAAAGGTCAAGGGAACTCAT+CGG	+	chr8.4:78212615-78212634	MS.gene020215:CDS	40.0%
	AAACCTGATGAGTAATGCTC+TGG	-	chr8.4:78214426-78214445	None:intergenic	40.0%
	AAGCGGAAGAATTTCCTTTC+TGG	-	chr8.4:78215148-78215167	None:intergenic	40.0%
	ACAGCCTAAGAAAAAGGTCA+AGG	+	chr8.4:78212604-78212623	MS.gene020215:CDS	40.0%
	ACCATCTTGGAGCACATATA+TGG	+	chr8.4:78214887-78214906	MS.gene020215:intron	40.0%
	ACTGGAGAATCAACAACGAA+TGG	+	chr8.4:78215264-78215283	MS.gene020215:CDS	40.0%
	ATCTTGGAGCACATATATGG+CGG	+	chr8.4:78214890-78214909	MS.gene020215:intron	40.0%
	ATGAGCACATAGAATATGGG+TGG	-	chr8.4:78214928-78214947	None:intergenic	40.0%
	ATGATGCCTTGTAGAAAAGG+AGG	+	chr8.4:78215321-78215340	MS.gene020215:CDS	40.0%
	ATGTGGATTTGTGATTCCAC+AGG	-	chr8.4:78214786-78214805	None:intergenic	40.0%
	ATTGGACGACTGTCTTACAA+TGG	+	chr8.4:78212936-78212955	MS.gene020215:CDS	40.0%
	CAAATGCCACAATTGATACC+TGG	-	chr8.4:78213251-78213270	None:intergenic	40.0%
	CAAGTGCATCCACAAATCTT+GGG	-	chr8.4:78212912-78212931	None:intergenic	40.0%
	CAGCCTAAGAAAAAGGTCAA+GGG	+	chr8.4:78212605-78212624	MS.gene020215:CDS	40.0%
	CCCACCATATAACTTTCTGT+TGG	-	chr8.4:78214625-78214644	None:intergenic	40.0%
	CCGAAAAATAAAGAAAGCCG+AGG	+	chr8.4:78212718-78212737	MS.gene020215:CDS	40.0%
	CTCTGGTCAGATTCATCAAA+AGG	-	chr8.4:78214409-78214428	None:intergenic	40.0%
	CTTTGCAACCATTTATGCAG+AGG	-	chr8.4:78215416-78215435	None:intergenic	40.0%
	GAGAATATGTTCAACCTCAG+TGG	+	chr8.4:78214566-78214585	MS.gene020215:CDS	40.0%
	GCCAACAGAAAGTTATATGG+TGG	+	chr8.4:78214621-78214640	MS.gene020215:CDS	40.0%
	GTTCTATGTTCTTCCAGCAA+AGG	-	chr8.4:78212683-78212702	None:intergenic	40.0%
	TAGACATGATTATTCGGCAG+AGG	+	chr8.4:78212798-78212817	MS.gene020215:CDS	40.0%
	TATCTCGTACTCACAAGTTG+AGG	+	chr8.4:78213193-78213212	MS.gene020215:CDS	40.0%
	TGATGCCTTGTAGAAAAGGA+GGG	+	chr8.4:78215322-78215341	MS.gene020215:CDS	40.0%
	TGTTCGCCAAGACTATCAAA+GGG	-	chr8.4:78214062-78214081	None:intergenic	40.0%
	TTGAGGGTCAGACAATAACA+TGG	+	chr8.4:78213342-78213361	MS.gene020215:CDS	40.0%
	TTGCTAGATTGGCACATGAA+TGG	+	chr8.4:78213160-78213179	MS.gene020215:intron	40.0%
!	AGGGTTTTACAAAGCCATGA+CGG	+	chr8.4:78215341-78215360	MS.gene020215:CDS	40.0%
!	CAGAAAGTTATATGGTGGGA+AGG	+	chr8.4:78214626-78214645	MS.gene020215:CDS	40.0%
!	GCGGAAATTCATGTTTCTGA+AGG	-	chr8.4:78213695-78213714	None:intergenic	40.0%
!	GTAAGACCCTTTGATAGTCT+TGG	+	chr8.4:78214053-78214072	MS.gene020215:intron	40.0%
!	GTTCCCTTGACCTTTTTCTT+AGG	-	chr8.4:78212611-78212630	None:intergenic	40.0%
!	TCTTTTCCAACACTAGGAAG+CGG	-	chr8.4:78215165-78215184	None:intergenic	40.0%
!	TGACCTTTTTCTTAGGCTGT+CGG	-	chr8.4:78212604-78212623	None:intergenic	40.0%
!	TGCTTACCTGTTTGCTAGAT+TGG	+	chr8.4:78213149-78213168	MS.gene020215:intron	40.0%
!	TTCGTGGTTTTTCTTTGCCT+CGG	-	chr8.4:78212738-78212757	None:intergenic	40.0%
!!	GAATATGGGTGGCTTTAATG+TGG	-	chr8.4:78214917-78214936	None:intergenic	40.0%
!!	TTTTGGTGGCACCATTTCAT+GGG	+	chr8.4:78214369-78214388	MS.gene020215:CDS	40.0%
!!!	TTTGTTATTCCGGCTTTTGG+TGG	+	chr8.4:78214355-78214374	MS.gene020215:CDS	40.0%
	AATTGCCACAGCCTCTTCTT+CGG	+	chr8.4:78213562-78213581	MS.gene020215:intron	45.0%
	ACAAAAGGTGACAAGTGAGG+CGG	-	chr8.4:78215060-78215079	None:intergenic	45.0%
	ACCATTTCATGGGAGGGAAA+GGG	+	chr8.4:78214379-78214398	MS.gene020215:CDS	45.0%
	ACGAACTTGCAAACAGGCTA+CGG	+	chr8.4:78212753-78212772	MS.gene020215:CDS	45.0%
	AGACTGATCGCATCAAACAG+CGG	+	chr8.4:78215546-78215565	MS.gene020215:CDS	45.0%
	AGCTTCAAGTATCAGTCCAG+AGG	-	chr8.4:78213805-78213824	None:intergenic	45.0%
	AGCTTGTACACAAATGCCGA+AGG	+	chr8.4:78213010-78213029	MS.gene020215:CDS	45.0%
	ATTCTTCCGCTTCCTAGTGT+TGG	+	chr8.4:78215156-78215175	MS.gene020215:CDS	45.0%
	CAAGCAGAGTCCAAGCAAAA+AGG	+	chr8.4:78213846-78213865	MS.gene020215:CDS	45.0%
	CACAAATGAAAGCAAGGGCT+CGG	-	chr8.4:78214503-78214522	None:intergenic	45.0%
	CATGTGCCAATCTAGCAAAC+AGG	-	chr8.4:78213158-78213177	None:intergenic	45.0%
	CCAAGATTTGTGGATGCACT+TGG	+	chr8.4:78212910-78212929	MS.gene020215:CDS	45.0%
	CCAAGTGCATCCACAAATCT+TGG	-	chr8.4:78212913-78212932	None:intergenic	45.0%
	CTTAGCAGGTGAAGATGAAG+AGG	+	chr8.4:78213968-78213987	MS.gene020215:CDS	45.0%
	GCCATATATGTGCTCCAAGA+TGG	-	chr8.4:78214891-78214910	None:intergenic	45.0%
	GCTTCAAGTATCAGTCCAGA+GGG	-	chr8.4:78213804-78213823	None:intergenic	45.0%
	GGGATCATGATATCTTCCTG+TGG	+	chr8.4:78214767-78214786	MS.gene020215:intron	45.0%
	GTGTTCGCCAAGACTATCAA+AGG	-	chr8.4:78214063-78214082	None:intergenic	45.0%
	TAGACGAAGCTAAGGGTCAA+AGG	+	chr8.4:78215579-78215598	MS.gene020215:CDS	45.0%
	TCACCTGCTAAGTCCTTAAC+CGG	-	chr8.4:78213960-78213979	None:intergenic	45.0%
	TCCAAGCAAAAAGGAGCAGA+TGG	+	chr8.4:78213855-78213874	MS.gene020215:CDS	45.0%
	TGTGGATGCACTTGGAGAAT+TGG	+	chr8.4:78212918-78212937	MS.gene020215:CDS	45.0%
!	ACCATCTGCTCCTTTTTGCT+TGG	-	chr8.4:78213859-78213878	None:intergenic	45.0%
!	ACCTTTTTCTTAGGCTGTCG+GGG	-	chr8.4:78212602-78212621	None:intergenic	45.0%
!	ATTTTCTTGCTCTCTGACGC+AGG	-	chr8.4:78212982-78213001	None:intergenic	45.0%
!	GACCTTTTTCTTAGGCTGTC+GGG	-	chr8.4:78212603-78212622	None:intergenic	45.0%
!	GGTGAAAGGCATATACTACC+AGG	+	chr8.4:78213230-78213249	MS.gene020215:CDS	45.0%
!	TAAACTTGGAGTATCCTCCC+AGG	+	chr8.4:78213613-78213632	MS.gene020215:CDS	45.0%
!	TGACCAGAGCATTACTCATC+AGG	+	chr8.4:78214420-78214439	MS.gene020215:CDS	45.0%
!!	AGGGCTCGGTGAGTATAAAA+AGG	-	chr8.4:78214489-78214508	None:intergenic	45.0%
!!	CTTTTGGTGGCACCATTTCA+TGG	+	chr8.4:78214368-78214387	MS.gene020215:CDS	45.0%
!!!	TTATTAAATTTAAAATTTAC+AGG	+	chr8.4:78214950-78214969	MS.gene020215:intron	5.0%
	ACAAACCGAAGAAGAGGCTG+TGG	-	chr8.4:78213570-78213589	None:intergenic	50.0%
	ACCCCGACAGCCTAAGAAAA+AGG	+	chr8.4:78212598-78212617	MS.gene020215:CDS	50.0%
	ATCCTCCCAGGCTTGATACT+CGG	+	chr8.4:78213625-78213644	MS.gene020215:CDS	50.0%
	ATTGTTGACAGGGATGCGCA+AGG	+	chr8.4:78214526-78214545	MS.gene020215:CDS	50.0%
	CACCATTTCATGGGAGGGAA+AGG	+	chr8.4:78214378-78214397	MS.gene020215:CDS	50.0%
	CACCTGCTAAGTCCTTAACC+GGG	-	chr8.4:78213959-78213978	None:intergenic	50.0%
	CATTTCATGGGAGGGAAAGG+GGG	+	chr8.4:78214381-78214400	MS.gene020215:CDS	50.0%
	CCATTTCATGGGAGGGAAAG+GGG	+	chr8.4:78214380-78214399	MS.gene020215:CDS	50.0%
	CGAACTTGCAAACAGGCTAC+GGG	+	chr8.4:78212754-78212773	MS.gene020215:CDS	50.0%
	GTAGCCTGTTTGCAAGTTCG+TGG	-	chr8.4:78212754-78212773	None:intergenic	50.0%
	GTTCTTCCAGCAAAGGTTCG+TGG	-	chr8.4:78212676-78212695	None:intergenic	50.0%
	TCCAACCGAGTATCAAGCCT+GGG	-	chr8.4:78213633-78213652	None:intergenic	50.0%
	TCTGCTGTTGTGGTACAGAC+TGG	+	chr8.4:78215246-78215265	MS.gene020215:CDS	50.0%
	TCTTCACCACGAACCTTTGC+TGG	+	chr8.4:78212667-78212686	MS.gene020215:CDS	50.0%
	TTCCAACCGAGTATCAAGCC+TGG	-	chr8.4:78213634-78213653	None:intergenic	50.0%
	TTTGTGCAGGCTGAGGTTGA+GGG	+	chr8.4:78213326-78213345	MS.gene020215:intron	50.0%
!	TTTTGTGCAGGCTGAGGTTG+AGG	+	chr8.4:78213325-78213344	MS.gene020215:intron	50.0%
!!	GAAATGGTGCCACCAAAAGC+CGG	-	chr8.4:78214367-78214386	None:intergenic	50.0%
	AAAGGTGACAAGTGAGGCGG+AGG	-	chr8.4:78215057-78215076	None:intergenic	55.0%
	AACCGAGTATCAAGCCTGGG+AGG	-	chr8.4:78213630-78213649	None:intergenic	55.0%
	CACCCGGTTAAGGACTTAGC+AGG	+	chr8.4:78213954-78213973	MS.gene020215:CDS	55.0%
	CCCCTTTCCCTCCCATGAAA+TGG	-	chr8.4:78214383-78214402	None:intergenic	55.0%
	CTTGCAAGCTGCTGCCAGAA+AGG	+	chr8.4:78215131-78215150	MS.gene020215:CDS	55.0%
	GGTGGCACCATTTCATGGGA+GGG	+	chr8.4:78214373-78214392	MS.gene020215:CDS	55.0%
	TCCCAGGCTTGATACTCGGT+TGG	+	chr8.4:78213629-78213648	MS.gene020215:CDS	55.0%
	TGGTGGCACCATTTCATGGG+AGG	+	chr8.4:78214372-78214391	MS.gene020215:CDS	55.0%
!	TCCAGAGGGTTTGCTCGCAT+TGG	-	chr8.4:78213790-78213809	None:intergenic	55.0%
!	TGCACTGATGCACCCGGTTA+AGG	+	chr8.4:78213944-78213963	MS.gene020215:CDS	55.0%
	ACAGGGATGCGCAAGGCCAT+AGG	+	chr8.4:78214533-78214552	MS.gene020215:CDS	60.0%
	CGGTTGGAAGCTTTCGCAGC+AGG	+	chr8.4:78213645-78213664	MS.gene020215:CDS	60.0%
	GCCAATGCGAGCAAACCCTC+TGG	+	chr8.4:78213786-78213805	MS.gene020215:CDS	60.0%
!	ACCTGGTGCACTGATGCACC+CGG	+	chr8.4:78213938-78213957	MS.gene020215:CDS	60.0%
	ACCGGGTGCATCAGTGCACC+AGG	-	chr8.4:78213942-78213961	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	78212359	78215607	78212359	ID=MS.gene020215
chr8.4	mRNA	78212359	78215607	78212359	ID=MS.gene020215.t1;Parent=MS.gene020215
chr8.4	exon	78212359	78212425	78212359	ID=MS.gene020215.t1.exon1;Parent=MS.gene020215.t1
chr8.4	CDS	78212359	78212425	78212359	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78212591	78212819	78212591	ID=MS.gene020215.t1.exon2;Parent=MS.gene020215.t1
chr8.4	CDS	78212591	78212819	78212591	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78212906	78213031	78212906	ID=MS.gene020215.t1.exon3;Parent=MS.gene020215.t1
chr8.4	CDS	78212906	78213031	78212906	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78213167	78213251	78213167	ID=MS.gene020215.t1.exon4;Parent=MS.gene020215.t1
chr8.4	CDS	78213167	78213251	78213167	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78213335	78213445	78213335	ID=MS.gene020215.t1.exon5;Parent=MS.gene020215.t1
chr8.4	CDS	78213335	78213445	78213335	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78213573	78213666	78213573	ID=MS.gene020215.t1.exon6;Parent=MS.gene020215.t1
chr8.4	CDS	78213573	78213666	78213573	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78213763	78214052	78213763	ID=MS.gene020215.t1.exon7;Parent=MS.gene020215.t1
chr8.4	CDS	78213763	78214052	78213763	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78214334	78214441	78214334	ID=MS.gene020215.t1.exon8;Parent=MS.gene020215.t1
chr8.4	CDS	78214334	78214441	78214334	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78214526	78214647	78214526	ID=MS.gene020215.t1.exon9;Parent=MS.gene020215.t1
chr8.4	CDS	78214526	78214647	78214526	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78215050	78215198	78215050	ID=MS.gene020215.t1.exon10;Parent=MS.gene020215.t1
chr8.4	CDS	78215050	78215198	78215050	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78215241	78215362	78215241	ID=MS.gene020215.t1.exon11;Parent=MS.gene020215.t1
chr8.4	CDS	78215241	78215362	78215241	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1
chr8.4	exon	78215521	78215607	78215521	ID=MS.gene020215.t1.exon12;Parent=MS.gene020215.t1
chr8.4	CDS	78215521	78215607	78215521	ID=cds.MS.gene020215.t1;Parent=MS.gene020215.t1

Gene Sequence

>MS.gene020215

ATGCTGCAAAATTTGTCACTAGGACACAAGCACTCAAGAAACTCCAAATCAGTCTACCTCTTTTCAGGTATTATTTGTATTTTCTAGCATTACTGATTTGTTGTCATTTTGAAAATGCATCTATATAATTTGCGACTCTGAAACGTTTGTGAAAATATAATGTGTTAAAGTGTTACTACTTCGATTCTTACATTGGCTTTTGCAAATATTAGGAAACTAAGCATTTGGAAAGGTGTAACACCCCGACAGCCTAAGAAAAAGGTCAAGGGAACTCATCGGACTTATTATCATTTGAAAGATGTTAGCTTTCTTCACCACGAACCTTTGCTGGAAGAACATAGAACAATTAGAGTACATGACCGAAAAATAAAGAAAGCCGAGGCAAAGAAAAACCACGAACTTGCAAACAGGCTACGGGAGAAAACACCAAAACCAAAGATAGACATGATTATTCGGCAGAGGTTTGTTTGCTTTTTGTGTTACTCAATTTATATCTTGTAGCTGGTGATAGATTTTCCAGTTTATTTGATTTTCCTATGTATTATAGGTACCCAAGATTTGTGGATGCACTTGGAGAATTGGACGACTGTCTTACAATGGTGCATCTCTTTGCAGCATTACCTGCGTCAGAGAGCAAGAAAATTGATGTAGAGCTTGTACACAAATGCCGAAGGTATATAATACTTGGAATTTGTGTAAATTTTGATCAGTATGTGTAACTTCTATATTTCCTTTAAGAAAGTGCAAATTTGAAGACAGTAAGATCCTATCTTCTTAATTTATTTATTCTTGCTTACCTGTTTGCTAGATTGGCACATGAATGGCAAGCATTCATATCTCGTACTCACAAGTTGAGGAAAACATATGTATCGGTGAAAGGCATATACTACCAGGTATCAATTGTGGCATTTGTAATGTTTATGATAAAAAAATTGTACATGTCATATGGATATTGATTGATTATATTTTTGTGCAGGCTGAGGTTGAGGGTCAGACAATAACATGGTTAACTCCTCATTTACTACAACAGGTTGTGTCTGATGATGTTGACATGCCTACCATGCTAAATTTTCTGCAATTTTACAAGGTCGGTTTCATATTAATGTTTATATTTAGGGTCTTTGTTCTGTTAATACATCAACATTCATGTGATCACAATTATAAGACTGTTGACATTTTACTAATATCCTTAACTTCATAATGAATTGCCACAGCCTCTTCTTCGGTTTGTGAATTTCCGCCTCTATCATTCTATAAACTTGGAGTATCCTCCCAGGCTTGATACTCGGTTGGAAGCTTTCGCAGCAGGTACCATTTTCTGAACTTAGATGGACCTTCAGAAACATGAATTTCCGCATATACTCTATTATATATCTACTGATCAATTCTCTTTTCAAACTGTAGATCTATATGCACTGTCAAAATATGCCAATGCGAGCAAACCCTCTGGACTGATACTTGAAGCTTCTCAGTTAGCTGAATCTAAACAAGCAGAGTCCAAGCAAAAAGGAGCAGATGGTGAAAATGAAAAATCAGAACTAAGACTTGCTCAACTTCACCACCAACTTCTTTCTAATGAACCTGGTGCACTGATGCACCCGGTTAAGGACTTAGCAGGTGAAGATGAAGAGGATCGAGATACAAGAGAATGTAAAAATCTCTTCAAAAATCTTAAATTCTTCTTGAGTAGGGAGGTAAGACCCTTTGATAGTCTTGGCGAACACATCTATTTCAAAGTATTGTTTCTGTGAATAATTGGGAAATCAAGGACACATCATTTGCTTAGTTTCATTTCATGTTTTTTTAAAATTAAATATATGAAATGAATCAATAATATATGGATGATGTGCAAGTGATACGAAAAAGCTTATTAGACTTTTTTTCTTGATGCTTTATTTCTTGAACTTATTTATAGAATCATTTTCATGATTGCTTGCCTTGACAATACTTGCACTGTTTTGGTCTAATTACTCAGGTACAAAGGGAATCATTACTTTTTGTTATTCCGGCTTTTGGTGGCACCATTTCATGGGAGGGAAAGGGGGCACCTTTTGATGAATCTGACCAGAGCATTACTCATCAGGTTTGTGATTAAGCATATAATCTTCTATGAGACAGATTTTCAAGCCTTTTTATACTCACCGAGCCCTTGCTTTCATTTGTGTAGATTGTTGACAGGGATGCGCAAGGCCATAGGTTTCTATCTAGAGAATATGTTCAACCTCAGTGGATATTTGATTGTGTGAATGCACGCATAATTTTGCCAACAGAAAGTTATATGGTGGGAAGGTAATTTGTTTCCATTTTCTGTGCTTATTATTTTGGGTTCATTAGGGTTCTTATTTGTTGTAGCAATCTTTGTAGGAGTGTGCTATATTTTATTCTGAGCAAATTAGTGTTTTCTTCAAGGGATCATGATATCTTCCTGTGGAATCACAAATCCACATATCCTTTCTTCTTTTCATCTTGCTATTAAAAAACTACCTTATATGGTGGTCTTGATCAATCCAATAGTTTTAGAAAATTAGACCATCTTGGAGCACATATATGGCGGTCCACATTAAAGCCACCCATATTCTATGTGCTCATGTTTATTAAATTTAAAATTTACAGGTATATAGAAAGCGAGTCTATGTGGTGTTTATGATAACTTCCCTAGTTAAAAACTTAAAATGTTTCTTTTGTTAACAGGATTCCTCCGCCTCACTTGTCACCTTTTGTTAACTATGACAAAGAAGGCTACATTCCCGACTATGCAAAGACTATCAAACACTTGCAAGCTGCTGCCAGAAAGGAAATTCTTCCGCTTCCTAGTGTTGGAAAAGAAGATTCACTTCAGAGTGCTATTACATATACTTCAGCAGATTCTATTGTCGAAAAAGAAACATCTGCTGTTGTGGTACAGACTGGAGAATCAACAACGAATGGTCAAGAAAATGTCGATAATGATATGAGTTTACTTATGATGCCTTGTAGAAAAGGAGGGTTTTACAAAGCCATGACGGTAAACTTTTTTAATCTCCTTATGCTTACTTGAGTTGCTTAATTCTTTGTCCTCTGCATAAATGGTTGCAAAGTGATTGATGGGTTAAGTATTTACAATTGGTTCTGAATTTCTTCAACTTTGTTTATTACTTATTTTGTTATAAATCTGTTATTCAGATATCTAATAAGCGCAAGCAGAATGAGACTGATCGCATCAAACAGCGGAAAATGAAATTAGACGAAGCTAAGGGTCAAAGGAGTTGA

Protein sequence

>MS.gene020215.t1

MLQNLSLGHKHSRNSKSVYLFSGVTPRQPKKKVKGTHRTYYHLKDVSFLHHEPLLEEHRTIRVHDRKIKKAEAKKNHELANRLREKTPKPKIDMIIRQRYPRFVDALGELDDCLTMVHLFAALPASESKKIDVELVHKCRRLAHEWQAFISRTHKLRKTYVSVKGIYYQAEVEGQTITWLTPHLLQQVVSDDVDMPTMLNFLQFYKPLLRFVNFRLYHSINLEYPPRLDTRLEAFAADLYALSKYANASKPSGLILEASQLAESKQAESKQKGADGENEKSELRLAQLHHQLLSNEPGALMHPVKDLAGEDEEDRDTRECKNLFKNLKFFLSREVQRESLLFVIPAFGGTISWEGKGAPFDESDQSITHQIVDRDAQGHRFLSREYVQPQWIFDCVNARIILPTESYMVGRIPPPHLSPFVNYDKEGYIPDYAKTIKHLQAAARKEILPLPSVGKEDSLQKTSAVVVQTGESTTNGQENVDNDMSLLMMPCRKGGFYKAMTISNKRKQNETDRIKQRKMKLDEAKGQRS