Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009656.t1 | AFK46684.1 | 92.4 | 276 | 19 | 2 | 1 | 276 | 1 | 274 | 1.60E-100 | 375.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009656.t1 | P55871 | 65.9 | 276 | 85 | 5 | 1 | 274 | 1 | 269 | 2.0e-80 | 300.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009656.t1 | I3T2E2 | 92.4 | 276 | 19 | 2 | 1 | 276 | 1 | 274 | 1.1e-100 | 375.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene025997 | MS.gene009656 | PPI |
| MS.gene009656 | MS.gene22877 | PPI |
| MS.gene063366 | MS.gene009656 | PPI |
| MS.gene009656 | MS.gene039110 | PPI |
| MS.gene060216 | MS.gene009656 | PPI |
| MS.gene066163 | MS.gene009656 | PPI |
| MS.gene040581 | MS.gene009656 | PPI |
| MS.gene009656 | MS.gene63721 | PPI |
| MS.gene009656 | MS.gene058030 | PPI |
| MS.gene009656 | MS.gene024538 | PPI |
| MS.gene003452 | MS.gene009656 | PPI |
| MS.gene009656 | MS.gene061898 | PPI |
| MS.gene52139 | MS.gene009656 | PPI |
| MS.gene009656 | MS.gene050556 | PPI |
| MS.gene009656 | MS.gene64519 | PPI |
| MS.gene018560 | MS.gene009656 | PPI |
| MS.gene09852 | MS.gene009656 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009656.t1 | MTR_5g075530 | 92.029 | 276 | 20 | 2 | 1 | 276 | 49 | 322 | 3.43e-162 | 453 |
| MS.gene009656.t1 | MTR_3g467620 | 78.467 | 274 | 51 | 5 | 1 | 274 | 1 | 266 | 5.39e-132 | 374 |
| MS.gene009656.t1 | MTR_5g075530 | 92.529 | 174 | 12 | 1 | 1 | 174 | 49 | 221 | 1.05e-89 | 266 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene009656.t1 | AT5G20920 | 66.545 | 275 | 83 | 4 | 1 | 273 | 1 | 268 | 9.20e-116 | 333 |
| MS.gene009656.t1 | AT5G20920 | 66.545 | 275 | 83 | 4 | 1 | 273 | 1 | 268 | 9.20e-116 | 333 |
| MS.gene009656.t1 | AT5G20920 | 66.545 | 275 | 82 | 5 | 1 | 273 | 1 | 267 | 1.67e-114 | 330 |
| MS.gene009656.t1 | AT3G07920 | 62.745 | 153 | 48 | 2 | 124 | 272 | 17 | 164 | 1.85e-59 | 186 |
| MS.gene009656.t1 | AT5G01940 | 50.704 | 142 | 65 | 3 | 120 | 260 | 72 | 209 | 5.10e-39 | 136 |
| MS.gene009656.t1 | AT5G01940 | 50.704 | 142 | 65 | 3 | 120 | 260 | 104 | 241 | 7.99e-39 | 136 |
| MS.gene009656.t1 | AT5G01940 | 48.077 | 156 | 72 | 4 | 120 | 268 | 72 | 225 | 5.33e-37 | 131 |
Find 57 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATTCCTGCAAAACTCTT+TGG | 0.299200 | 5.3:+60537917 | None:intergenic |
| TTGGTTGTGAAGGGAAGATT+TGG | 0.327299 | 5.3:-60537941 | MS.gene009656:CDS |
| TGGAGAGGCAGTTGAAGATT+TGG | 0.342163 | 5.3:-60552533 | MS.gene009656:CDS |
| CTTTGCAGCCTCGTTATCCT+TGG | 0.346996 | 5.3:-60552360 | MS.gene009656:CDS |
| TGATGAATCAGTGGATAAAT+TGG | 0.362248 | 5.3:-60552895 | MS.gene009656:CDS |
| CGCCGATCTCCAGCAAGATC+TGG | 0.373236 | 5.3:+60538464 | None:intergenic |
| GATGAATATGTCATTTGTAT+TGG | 0.394913 | 5.3:-60537150 | MS.gene009656:CDS |
| GCAACAAAACCAGCTTTGAT+TGG | 0.396441 | 5.3:+60536960 | None:intergenic |
| TGTACTAGTGGTTATATAGA+TGG | 0.407956 | 5.3:-60537974 | MS.gene009656:CDS |
| TATTGCTTGCAGTGTGGATC+AGG | 0.417838 | 5.3:-60536996 | MS.gene009656:intron |
| TTGTATTGGATGCAAGAGTC+TGG | 0.425485 | 5.3:-60537136 | MS.gene009656:CDS |
| GAACATAATCCAGATCTTGC+TGG | 0.451726 | 5.3:-60538473 | MS.gene009656:CDS |
| TATAGATGGACAGCAAAGAT+TGG | 0.470808 | 5.3:-60537960 | MS.gene009656:CDS |
| TATTCACTTGCAGTTTCTGA+TGG | 0.471762 | 5.3:-60552743 | MS.gene009656:intron |
| AAGAAAGCCATAACATGGTC+TGG | 0.472766 | 5.3:+60538010 | None:intergenic |
| TGAAACACCAAGTGGTTTGA+AGG | 0.477271 | 5.3:-60554028 | MS.gene009656:CDS |
| TTTGCAGCCTCGTTATCCTT+GGG | 0.480164 | 5.3:-60552359 | MS.gene009656:CDS |
| CTTCTTTCTCCGGTGTGAGA+AGG | 0.497603 | 5.3:-60537085 | MS.gene009656:intron |
| CAGCCTCGTTATCCTTGGGA+AGG | 0.501422 | 5.3:-60552355 | MS.gene009656:CDS |
| AGAACCGTCTCTTCTTTCTC+CGG | 0.503608 | 5.3:-60537095 | MS.gene009656:CDS |
| TCGACCGCCAGACCATGTTA+TGG | 0.515326 | 5.3:-60538017 | MS.gene009656:CDS |
| ATCCAGATCTTGCTGGAGAT+CGG | 0.530264 | 5.3:-60538466 | MS.gene009656:CDS |
| TTGCTCGTGTTGGCGGCAGA+AGG | 0.531864 | 5.3:-60536941 | MS.gene009656:CDS |
| TCTGGACACATTACTTTCAA+AGG | 0.537856 | 5.3:-60537118 | MS.gene009656:CDS |
| TGACCGTGATTATACATATG+AGG | 0.537992 | 5.3:-60552329 | MS.gene009656:CDS |
| TCAGTTGCTCCAATCAAAGC+TGG | 0.541836 | 5.3:-60536969 | MS.gene009656:CDS |
| GAGAAGATAGAAAATCTGTC+AGG | 0.544015 | 5.3:-60552870 | MS.gene009656:intron |
| GTGCCTTCACGGCGAACTCG+AGG | 0.547950 | 5.3:+60538422 | None:intergenic |
| CCTCCTCGAGTTCGCCGTGA+AGG | 0.548479 | 5.3:-60538425 | MS.gene009656:CDS |
| ATTCACTTGCAGTTTCTGAT+GGG | 0.549550 | 5.3:-60552742 | MS.gene009656:intron |
| TCTTCAACTGCCTCTCCACT+AGG | 0.552585 | 5.3:+60552538 | None:intergenic |
| ACTTCCTCCTTCAAACCACT+TGG | 0.569420 | 5.3:+60554021 | None:intergenic |
| ATCGGCGAAGAACAGTTATG+AGG | 0.576484 | 5.3:-60538448 | MS.gene009656:CDS |
| AGCCTCGTTATCCTTGGGAA+GGG | 0.579244 | 5.3:-60552354 | MS.gene009656:CDS |
| CAGCAAAGATTGGTTGTGAA+GGG | 0.579584 | 5.3:-60537950 | MS.gene009656:CDS |
| ACAGCAAAGATTGGTTGTGA+AGG | 0.582650 | 5.3:-60537951 | MS.gene009656:CDS |
| GAGGCAGTTGAAGATTTGGA+TGG | 0.585613 | 5.3:-60552529 | MS.gene009656:intron |
| TTTGGTACAGCTTCTAGGCA+GGG | 0.597741 | 5.3:-60538513 | MS.gene009656:intron |
| CTTGCTGAACTATGTACTAG+TGG | 0.601332 | 5.3:-60537986 | MS.gene009656:CDS |
| AGTAAGGCCAAATCATGCCT+CGG | 0.608563 | 5.3:+60536914 | None:intergenic |
| TACTGCAGATGATGAATCAG+TGG | 0.608690 | 5.3:-60552904 | MS.gene009656:CDS |
| TTAAATGATATTGATGAGAG+TGG | 0.613941 | 5.3:-60552562 | MS.gene009656:CDS |
| CCTCCTCATATGTATAATCA+CGG | 0.618081 | 5.3:+60552326 | None:intergenic |
| CTCCCTTCCCAAGGATAACG+AGG | 0.620050 | 5.3:+60552352 | None:intergenic |
| CCTTCACGGCGAACTCGAGG+AGG | 0.625429 | 5.3:+60538425 | None:intergenic |
| CAAGCAAGAAAGCCATAACA+TGG | 0.625960 | 5.3:+60538005 | None:intergenic |
| AATACTTACTGATATATCGT+CGG | 0.636337 | 5.3:+60537894 | None:intergenic |
| TCACGGTCACTCCCTTCCCA+AGG | 0.642597 | 5.3:+60552343 | None:intergenic |
| GAAAATTCACCTTCTCACAC+CGG | 0.642926 | 5.3:+60537076 | None:intergenic |
| AACACCAAGTGGTTTGAAGG+AGG | 0.644979 | 5.3:-60554025 | MS.gene009656:CDS |
| ATTGATGAGAGTGGTCCTAG+TGG | 0.645228 | 5.3:-60552553 | MS.gene009656:CDS |
| TGAGAGTGGTCCTAGTGGAG+AGG | 0.666586 | 5.3:-60552548 | MS.gene009656:CDS |
| CACACCGGAGAAAGAAGAGA+CGG | 0.669379 | 5.3:+60537091 | None:intergenic |
| TGGCGGCAGAAGGAATGCCG+AGG | 0.670535 | 5.3:-60536931 | MS.gene009656:CDS |
| CCGTGATTATACATATGAGG+AGG | 0.685858 | 5.3:-60552326 | MS.gene009656:intron |
| AAAGCCATAACATGGTCTGG+CGG | 0.709853 | 5.3:+60538013 | None:intergenic |
| ATGGCAGATGAAACACCAAG+TGG | 0.740265 | 5.3:-60554036 | MS.gene009656:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 60536926 | 60554058 | 60536926 | ID=MS.gene009656 |
| chr5.3 | mRNA | 60536926 | 60554058 | 60536926 | ID=MS.gene009656.t1;Parent=MS.gene009656 |
| chr5.3 | exon | 60554014 | 60554058 | 60554014 | ID=MS.gene009656.t1.exon1;Parent=MS.gene009656.t1 |
| chr5.3 | CDS | 60554014 | 60554058 | 60554014 | ID=cds.MS.gene009656.t1;Parent=MS.gene009656.t1 |
| chr5.3 | exon | 60552871 | 60552982 | 60552871 | ID=MS.gene009656.t1.exon2;Parent=MS.gene009656.t1 |
| chr5.3 | CDS | 60552871 | 60552982 | 60552871 | ID=cds.MS.gene009656.t1;Parent=MS.gene009656.t1 |
| chr5.3 | exon | 60552694 | 60552752 | 60552694 | ID=MS.gene009656.t1.exon3;Parent=MS.gene009656.t1 |
| chr5.3 | CDS | 60552694 | 60552752 | 60552694 | ID=cds.MS.gene009656.t1;Parent=MS.gene009656.t1 |
| chr5.3 | exon | 60552530 | 60552599 | 60552530 | ID=MS.gene009656.t1.exon4;Parent=MS.gene009656.t1 |
| chr5.3 | CDS | 60552530 | 60552599 | 60552530 | ID=cds.MS.gene009656.t1;Parent=MS.gene009656.t1 |
| chr5.3 | exon | 60552327 | 60552415 | 60552327 | ID=MS.gene009656.t1.exon5;Parent=MS.gene009656.t1 |
| chr5.3 | CDS | 60552327 | 60552415 | 60552327 | ID=cds.MS.gene009656.t1;Parent=MS.gene009656.t1 |
| chr5.3 | exon | 60538380 | 60538525 | 60538380 | ID=MS.gene009656.t1.exon6;Parent=MS.gene009656.t1 |
| chr5.3 | CDS | 60538380 | 60538525 | 60538380 | ID=cds.MS.gene009656.t1;Parent=MS.gene009656.t1 |
| chr5.3 | exon | 60537903 | 60538045 | 60537903 | ID=MS.gene009656.t1.exon7;Parent=MS.gene009656.t1 |
| chr5.3 | CDS | 60537903 | 60538045 | 60537903 | ID=cds.MS.gene009656.t1;Parent=MS.gene009656.t1 |
| chr5.3 | exon | 60537086 | 60537171 | 60537086 | ID=MS.gene009656.t1.exon8;Parent=MS.gene009656.t1 |
| chr5.3 | CDS | 60537086 | 60537171 | 60537086 | ID=cds.MS.gene009656.t1;Parent=MS.gene009656.t1 |
| chr5.3 | exon | 60536926 | 60537006 | 60536926 | ID=MS.gene009656.t1.exon9;Parent=MS.gene009656.t1 |
| chr5.3 | CDS | 60536926 | 60537006 | 60536926 | ID=cds.MS.gene009656.t1;Parent=MS.gene009656.t1 |
| Gene Sequence |
| Protein sequence |
