Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012032.t1 | XP_024629315.1 | 91.8 | 463 | 37 | 1 | 1 | 463 | 1 | 462 | 2.80E-235 | 824.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012032.t1 | Q557M8 | 24.8 | 391 | 211 | 11 | 8 | 378 | 14 | 341 | 1.5e-11 | 72.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012032.t1 | G7LDX0 | 87.9 | 480 | 40 | 3 | 1 | 463 | 1 | 479 | 3.1e-228 | 800.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene39443 | MS.gene012032 | PPI |
| MS.gene031871 | MS.gene012032 | PPI |
| MS.gene052488 | MS.gene012032 | PPI |
| MS.gene33782 | MS.gene012032 | PPI |
| MS.gene08242 | MS.gene012032 | PPI |
| MS.gene007951 | MS.gene012032 | PPI |
| MS.gene007950 | MS.gene012032 | PPI |
| MS.gene31944 | MS.gene012032 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012032.t1 | MTR_8g077000 | 87.917 | 480 | 40 | 3 | 1 | 463 | 1 | 479 | 0.0 | 798 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012032.t1 | AT1G03280 | 57.792 | 462 | 163 | 13 | 1 | 445 | 12 | 458 | 4.37e-171 | 489 |
| MS.gene012032.t1 | AT1G03280 | 57.792 | 462 | 163 | 13 | 1 | 445 | 12 | 458 | 4.37e-171 | 489 |
| MS.gene012032.t1 | AT1G03280 | 57.792 | 462 | 163 | 13 | 1 | 445 | 12 | 458 | 4.37e-171 | 489 |
| MS.gene012032.t1 | AT4G20340 | 52.088 | 455 | 194 | 10 | 1 | 445 | 13 | 453 | 1.58e-139 | 409 |
| MS.gene012032.t1 | AT4G20340 | 52.088 | 455 | 194 | 10 | 1 | 445 | 13 | 453 | 1.58e-139 | 409 |
| MS.gene012032.t1 | AT4G20810 | 47.436 | 468 | 180 | 14 | 8 | 462 | 1 | 415 | 7.42e-130 | 382 |
| MS.gene012032.t1 | AT4G20340 | 52.206 | 408 | 172 | 9 | 48 | 445 | 4 | 398 | 3.13e-121 | 360 |
Find 97 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATCACTTCTTCCAGTTT+TGG | 0.162141 | 8.2:-28603465 | None:intergenic |
| GACTTGCCTGTTCCAAAATT+TGG | 0.170144 | 8.2:+28612070 | MS.gene012032:CDS |
| AACTGACAGTACATCTTTAA+AGG | 0.175603 | 8.2:+28613463 | MS.gene012032:CDS |
| GCTTGAAGACTGCCAAATTT+TGG | 0.179091 | 8.2:-28612082 | None:intergenic |
| CGACCGATTTGGTCTATTAG+AGG | 0.304159 | 8.2:-28612040 | None:intergenic |
| GAAGCTCGGGCTAGTGCAGC+TGG | 0.317114 | 8.2:+28612109 | MS.gene012032:CDS |
| ATCTGCTGTGTTCACATCTT+GGG | 0.327954 | 8.2:-28613856 | None:intergenic |
| CATCTGCTGTGTTCACATCT+TGG | 0.328566 | 8.2:-28613857 | None:intergenic |
| TAATGCTGGTGATTCTAATC+TGG | 0.336277 | 8.2:+28612150 | MS.gene012032:CDS |
| GAAGATAATCAGCCCAAAAC+TGG | 0.349455 | 8.2:+28603452 | MS.gene012032:CDS |
| GTGAAATTCGTACTTATTGT+AGG | 0.350382 | 8.2:-28603234 | None:intergenic |
| GGGCTGATTATCTTCTTTAC+CGG | 0.354315 | 8.2:-28603444 | None:intergenic |
| ACGGCGGCGCGAAAAGTTAA+AGG | 0.358209 | 8.2:+28605836 | MS.gene012032:CDS |
| GCATAAGCTGAAAGATGAAT+TGG | 0.358679 | 8.2:+28605515 | MS.gene012032:CDS |
| GATTCTAATCTGGGATATAA+TGG | 0.362821 | 8.2:+28612160 | MS.gene012032:CDS |
| TGGGAAGAGGCTCCTGTTAG+AGG | 0.376308 | 8.2:+28613954 | MS.gene012032:CDS |
| AGATTATCACTAGAGTTAAT+AGG | 0.382364 | 8.2:+28604448 | MS.gene012032:CDS |
| TCATCTTCATTGTCTTCTGC+TGG | 0.387140 | 8.2:-28616226 | None:intergenic |
| GTCTTCAAGCATGGGAAGCT+CGG | 0.396973 | 8.2:+28612095 | MS.gene012032:CDS |
| TATGTACAAATTGTGGAAAA+AGG | 0.405813 | 8.2:+28605565 | MS.gene012032:CDS |
| GAATAAAATGTACCTCTAAC+AGG | 0.409316 | 8.2:-28613966 | None:intergenic |
| GCAGCTGGGCGTGCAGCTAA+TGG | 0.419366 | 8.2:+28612124 | MS.gene012032:CDS |
| AGTCTTCTACCCGACCGATT+TGG | 0.429028 | 8.2:-28612051 | None:intergenic |
| ACAGCGCGCAGAGGTGAAGC+AGG | 0.440102 | 8.2:+28613532 | MS.gene012032:CDS |
| GTTGCTTTGTATGCAATTTG+AGG | 0.441479 | 8.2:-28604392 | None:intergenic |
| TGCAGATATATAAAGGGGTT+AGG | 0.445590 | 8.2:+28605472 | MS.gene012032:intron |
| TTCCATTGTGAATGTTGTAA+TGG | 0.457370 | 8.2:+28605744 | MS.gene012032:CDS |
| TCGACTGGGAAGAAGGTTGA+TGG | 0.459603 | 8.2:+28616251 | MS.gene012032:CDS |
| TTTATAGGATGAGTATATCA+AGG | 0.460980 | 8.2:+28613776 | MS.gene012032:intron |
| CGATTGGAAGTACTGCTAGA+AGG | 0.461514 | 8.2:-28613882 | None:intergenic |
| TAAAGGAAATGGAGGTTACA+AGG | 0.463278 | 8.2:+28616177 | MS.gene012032:intron |
| AGGAAAAGATGGAGAAGAAA+AGG | 0.464213 | 8.2:+28605240 | MS.gene012032:CDS |
| ATTGTCTTCTGCTGGAGCAT+CGG | 0.466214 | 8.2:-28616218 | None:intergenic |
| ACCTTGGTATCTCCGCTGTA+TGG | 0.489229 | 8.2:-28612193 | None:intergenic |
| AAAGGACATGCTTCAAAAGA+TGG | 0.489353 | 8.2:+28605854 | MS.gene012032:CDS |
| CCTCCATGGATGATTACATC+AGG | 0.493168 | 8.2:+28613491 | MS.gene012032:CDS |
| AATGCTGGTGATTCTAATCT+GGG | 0.496431 | 8.2:+28612151 | MS.gene012032:CDS |
| GAATATATATGTACAAATTG+TGG | 0.502239 | 8.2:+28605558 | MS.gene012032:CDS |
| TTTCATGCCAACTTGGCGAT+TGG | 0.507113 | 8.2:-28613898 | None:intergenic |
| TCTTCAAGCATGGGAAGCTC+GGG | 0.510368 | 8.2:+28612096 | MS.gene012032:CDS |
| ATGTTGTAATGGAAAATTAG+AGG | 0.514079 | 8.2:+28605755 | MS.gene012032:CDS |
| TGAAATTCGTACTTATTGTA+GGG | 0.520023 | 8.2:-28603233 | None:intergenic |
| ACAATGAAGATGATGTCGAC+TGG | 0.524851 | 8.2:+28616236 | MS.gene012032:CDS |
| GCTTTCTATGATGATTATAC+CGG | 0.526519 | 8.2:+28603425 | MS.gene012032:CDS |
| CAGGTGGTGGTTGACTTCAA+TGG | 0.533139 | 8.2:+28613407 | MS.gene012032:intron |
| TCATCCTTGATGCCCTCACC+AGG | 0.539237 | 8.2:+28603504 | MS.gene012032:CDS |
| CACTAGAGTTAATAGGAAAG+AGG | 0.545963 | 8.2:+28604455 | MS.gene012032:CDS |
| AGTACTTCCAATCGCCAAGT+TGG | 0.547271 | 8.2:+28613891 | MS.gene012032:CDS |
| AATTTGGCAGTCTTCAAGCA+TGG | 0.551091 | 8.2:+28612086 | MS.gene012032:CDS |
| AAGCCTCTAATAGACCAAAT+CGG | 0.551332 | 8.2:+28612037 | MS.gene012032:CDS |
| ATACCTGATGTAATCATCCA+TGG | 0.551552 | 8.2:-28613494 | None:intergenic |
| ATTTGGCAGTCTTCAAGCAT+GGG | 0.552152 | 8.2:+28612087 | MS.gene012032:CDS |
| CTCTAATAGACCAAATCGGT+CGG | 0.554167 | 8.2:+28612041 | MS.gene012032:CDS |
| GATGCTACGAAAGGAAAAGA+TGG | 0.554983 | 8.2:+28605229 | MS.gene012032:CDS |
| AAGCTCGGGCTAGTGCAGCT+GGG | 0.559290 | 8.2:+28612110 | MS.gene012032:CDS |
| TGAACCTGGTGAGGGCATCA+AGG | 0.563106 | 8.2:-28603508 | None:intergenic |
| GATGATGTCGACTGGGAAGA+AGG | 0.566390 | 8.2:+28616244 | MS.gene012032:CDS |
| CAATGAAGATGATGTCGACT+GGG | 0.567076 | 8.2:+28616237 | MS.gene012032:CDS |
| TCTAATAGACCAAATCGGTC+GGG | 0.571691 | 8.2:+28612042 | MS.gene012032:CDS |
| TTGATAAATTGAACCTGGTG+AGG | 0.571718 | 8.2:-28603517 | None:intergenic |
| GCTGCTCAAGAAGGTGGAGA+TGG | 0.572571 | 8.2:+28605795 | MS.gene012032:CDS |
| AAGTTAGCTGCTCAAGAAGG+TGG | 0.576893 | 8.2:+28605789 | MS.gene012032:CDS |
| ATCAGCTGTAGCAGCTACAG+CGG | 0.579271 | 8.2:-28605209 | None:intergenic |
| AAACGCAAGGAAGATGATGA+TGG | 0.583208 | 8.2:+28613924 | MS.gene012032:CDS |
| TGATAAATTGAACCTGGTGA+GGG | 0.583295 | 8.2:-28603516 | None:intergenic |
| TCTGCTGTGTTCACATCTTG+GGG | 0.584121 | 8.2:-28613855 | None:intergenic |
| GTATCTCCGCTGTATGGCAG+TGG | 0.587254 | 8.2:-28612187 | None:intergenic |
| TGTACATATATATTCTCGAA+CGG | 0.588463 | 8.2:-28605550 | None:intergenic |
| GTGTTGCTTCTTAAGTAAAG+CGG | 0.592790 | 8.2:-28613808 | None:intergenic |
| GTCTGTGTATTGAGCTGCTG+TGG | 0.592950 | 8.2:-28613582 | None:intergenic |
| GAAAAGTTAGCTGCTCAAGA+AGG | 0.594454 | 8.2:+28605786 | MS.gene012032:CDS |
| GTTGACTTCAATGGTACTGA+AGG | 0.595897 | 8.2:+28613416 | MS.gene012032:CDS |
| ATGGATTAGAGAAGAAGACT+TGG | 0.600807 | 8.2:+28604362 | MS.gene012032:CDS |
| GGTACTGAAGGCAAAGGAGA+GGG | 0.602003 | 8.2:+28613428 | MS.gene012032:CDS |
| CTGTTGTCTAGATTATGCAC+AGG | 0.608523 | 8.2:+28605285 | MS.gene012032:CDS |
| TTCCATTACAACATTCACAA+TGG | 0.611518 | 8.2:-28605746 | None:intergenic |
| TGGCATGAAATCAAAACGCA+AGG | 0.615437 | 8.2:+28613911 | MS.gene012032:CDS |
| GAGGGCATCAAGGATGACTA+CGG | 0.617138 | 8.2:-28603498 | None:intergenic |
| GCAGCTAATGGAGATAATGC+TGG | 0.617199 | 8.2:+28612136 | MS.gene012032:CDS |
| GAGATGAGAATGCAAGAAGA+CGG | 0.619322 | 8.2:+28605817 | MS.gene012032:CDS |
| AGATGATGATGGTACTGAGT+GGG | 0.622629 | 8.2:+28613935 | MS.gene012032:CDS |
| TAGAGAAGAAGACTTGGCAA+AGG | 0.625307 | 8.2:+28604368 | MS.gene012032:CDS |
| TGATGGTACTGAGTGGGAAG+AGG | 0.628964 | 8.2:+28613941 | MS.gene012032:CDS |
| AGGAAATGGAGGTTACAAGG+TGG | 0.630329 | 8.2:+28616180 | MS.gene012032:intron |
| GCCATACAGCGGAGATACCA+AGG | 0.630579 | 8.2:+28612192 | MS.gene012032:CDS |
| TTCAATGGTACTGAAGGCAA+AGG | 0.631073 | 8.2:+28613422 | MS.gene012032:CDS |
| TGGTACTGAAGGCAAAGGAG+AGG | 0.643856 | 8.2:+28613427 | MS.gene012032:CDS |
| GCTACAGCTGATGCTACGAA+AGG | 0.657325 | 8.2:+28605220 | MS.gene012032:CDS |
| GGACATGCTTCAAAAGATGG+AGG | 0.658761 | 8.2:+28605857 | MS.gene012032:CDS |
| TACGAAGGAACAGCGCGCAG+AGG | 0.662953 | 8.2:+28613523 | MS.gene012032:CDS |
| ATCAGGTATGAATCTTACGA+AGG | 0.688860 | 8.2:+28613508 | MS.gene012032:CDS |
| ATGAGAATGCAAGAAGACGG+CGG | 0.703092 | 8.2:+28605820 | MS.gene012032:CDS |
| GGAGCACCACTGCCATACAG+CGG | 0.717941 | 8.2:+28612181 | MS.gene012032:CDS |
| ACTGGAAGAAGTGATAACAG+AGG | 0.728511 | 8.2:+28603470 | MS.gene012032:CDS |
| CCTGATGTAATCATCCATGG+AGG | 0.742710 | 8.2:-28613491 | None:intergenic |
| ACAACGCTTTGGATGCACTG+CGG | 0.757151 | 8.2:+28605694 | MS.gene012032:CDS |
| AAGATGATGATGGTACTGAG+TGG | 0.777201 | 8.2:+28613934 | MS.gene012032:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 28603224 | 28616270 | 28603224 | ID=MS.gene012032 |
| chr8.2 | mRNA | 28603224 | 28616270 | 28603224 | ID=MS.gene012032.t1;Parent=MS.gene012032 |
| chr8.2 | exon | 28603224 | 28603243 | 28603224 | ID=MS.gene012032.t1.exon1;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28603224 | 28603243 | 28603224 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| chr8.2 | exon | 28603403 | 28603525 | 28603403 | ID=MS.gene012032.t1.exon2;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28603403 | 28603525 | 28603403 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| chr8.2 | exon | 28604356 | 28604476 | 28604356 | ID=MS.gene012032.t1.exon3;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28604356 | 28604476 | 28604356 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| chr8.2 | exon | 28605181 | 28605306 | 28605181 | ID=MS.gene012032.t1.exon4;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28605181 | 28605306 | 28605181 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| chr8.2 | exon | 28605477 | 28605586 | 28605477 | ID=MS.gene012032.t1.exon5;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28605477 | 28605586 | 28605477 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| chr8.2 | exon | 28605692 | 28605878 | 28605692 | ID=MS.gene012032.t1.exon6;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28605692 | 28605878 | 28605692 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| chr8.2 | exon | 28612028 | 28612213 | 28612028 | ID=MS.gene012032.t1.exon7;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28612028 | 28612213 | 28612028 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| chr8.2 | exon | 28613410 | 28613646 | 28613410 | ID=MS.gene012032.t1.exon8;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28613410 | 28613646 | 28613410 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| chr8.2 | exon | 28613783 | 28613975 | 28613783 | ID=MS.gene012032.t1.exon9;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28613783 | 28613975 | 28613783 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| chr8.2 | exon | 28616182 | 28616270 | 28616182 | ID=MS.gene012032.t1.exon10;Parent=MS.gene012032.t1 |
| chr8.2 | CDS | 28616182 | 28616270 | 28616182 | ID=cds.MS.gene012032.t1;Parent=MS.gene012032.t1 |
| Gene Sequence |
| Protein sequence |
