AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77503


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77503.t1 MTR_3g083760 82.673 202 16 1 1 183 1 202 1.18e-94 273
MS.gene77503.t1 MTR_3g083760 87.413 143 16 1 1 141 1 143 6.48e-81 236
MS.gene77503.t1 MTR_8g085560 50.829 181 83 3 9 183 14 194 5.45e-66 201
MS.gene77503.t1 MTR_7g014880 42.222 180 93 3 8 176 12 191 3.99e-41 137
MS.gene77503.t1 MTR_8g446830 39.487 195 105 6 1 182 1 195 1.05e-36 126
MS.gene77503.t1 MTR_1g103450 31.333 150 79 4 12 161 15 140 7.10e-13 63.5
MS.gene77503.t1 MTR_1g103490 36.667 90 55 2 12 101 10 97 2.34e-12 62.0
MS.gene77503.t1 MTR_1g103470 39.189 74 42 2 28 101 25 95 1.66e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77503.t1 AT1G55260 43.258 178 93 4 8 181 53 226 6.91e-44 145
MS.gene77503.t1 AT1G55260 43.258 178 90 5 8 181 53 223 2.21e-43 144
MS.gene77503.t1 AT2G44300 39.796 196 96 5 7 183 11 203 1.38e-40 136
MS.gene77503.t1 AT2G44290 38.579 197 96 4 9 183 11 204 1.60e-40 136
MS.gene77503.t1 AT3G58550 38.926 149 84 4 4 145 9 157 6.51e-30 108
MS.gene77503.t1 AT1G03103 31.915 94 64 0 8 101 2 95 1.91e-14 68.2
MS.gene77503.t1 AT5G09370 37.736 106 61 2 24 127 21 123 3.16e-14 66.6
MS.gene77503.t1 AT5G09370 37.600 125 64 5 24 138 21 141 3.74e-14 67.0
MS.gene77503.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77503.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77503.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77503.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77503.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77503.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77503.t1 AT5G09370 39.175 97 54 2 24 118 21 114 6.05e-14 66.6
MS.gene77503.t1 AT1G73890 39.189 74 45 0 31 104 30 103 7.41e-14 67.0
MS.gene77503.t1 AT3G22600 28.188 149 95 5 10 150 6 150 2.51e-12 62.4
MS.gene77503.t1 AT4G14815 30.000 90 62 1 12 101 7 95 4.42e-11 58.9

Find 51 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAATTACCGCTGGAAAT+AGG 0.158824 3.2:+72340472 None:intergenic
TCTTATCAAGGACTTGTTTA+AGG 0.173161 3.2:+72341168 None:intergenic
ACCTTAGCTTCAGGGGATTT+AGG 0.238114 3.2:+72340538 None:intergenic
AATAATTACCGCTGGAAATA+GGG 0.274317 3.2:+72340473 None:intergenic
AAGGAGGTGGCTGGTGGCAG+AGG 0.280788 3.2:-72340313 MS.gene77503:CDS
ATTCAACTTGATACCAAGTT+TGG 0.282616 3.2:+72341103 None:intergenic
GATAAACTTGTTACTCTTGC+TGG 0.316761 3.2:-72341248 MS.gene77503:CDS
CCTTCAAACACCTTAGCTTC+AGG 0.355634 3.2:+72340529 None:intergenic
TTCAACTTGATACCAAGTTT+GGG 0.358716 3.2:+72341104 None:intergenic
ACACATTCAGTTATATTAGA+TGG 0.359046 3.2:+72341041 None:intergenic
TGGATTGGGAAGGAGGTGGC+TGG 0.375917 3.2:-72340322 MS.gene77503:CDS
AGGGGAGGAACTGTTTGTTT+TGG 0.377595 3.2:+72340492 None:intergenic
GAAAAGAGTGGTGGTGGATT+GGG 0.380896 3.2:-72340336 MS.gene77503:CDS
TGCATTATTATTTCTACTGT+TGG 0.388007 3.2:-72341318 MS.gene77503:CDS
TTATTATTTCTACTGTTGGC+TGG 0.403176 3.2:-72341314 MS.gene77503:CDS
CTTATCAAGGACTTGTTTAA+GGG 0.407090 3.2:+72341169 None:intergenic
GATGGTATGTGACAAGCGTT+TGG 0.413443 3.2:+72341059 None:intergenic
GGAAAAGAGTGGTGGTGGAT+TGG 0.435272 3.2:-72340337 MS.gene77503:CDS
GCTAAGGTGTTTGAAGGACT+TGG 0.437073 3.2:-72340523 MS.gene77503:CDS
GAGGTGGCTGGTGGCAGAGG+TGG 0.447551 3.2:-72340310 MS.gene77503:CDS
AAGTGAGATATGAAAAGAAA+TGG 0.450601 3.2:+72340273 None:intergenic
GGAAGTACAACAATCTATAG+TGG 0.456166 3.2:+72341190 None:intergenic
CTTCAAACACCTTAGCTTCA+GGG 0.472455 3.2:+72340530 None:intergenic
ACCTAAATCCCCTGAAGCTA+AGG 0.474094 3.2:-72340539 MS.gene77503:CDS
CCTGAAGCTAAGGTGTTTGA+AGG 0.476875 3.2:-72340529 MS.gene77503:CDS
GATCGCAATGACCCCAAACT+TGG 0.492547 3.2:-72341116 MS.gene77503:CDS
GTGGTGGATTGGGAAGGAGG+TGG 0.495091 3.2:-72340326 MS.gene77503:CDS
GTGGTGGTGGATTGGGAAGG+AGG 0.496077 3.2:-72340329 MS.gene77503:CDS
TCTGAGGAAAAGAGTGGTGG+TGG 0.499326 3.2:-72340342 MS.gene77503:CDS
TGTTTAAGGTTCTGCTGAGA+GGG 0.503753 3.2:-72340379 MS.gene77503:intron
GAAGTACAACAATCTATAGT+GGG 0.507467 3.2:+72341191 None:intergenic
ATTGGGAAGGAGGTGGCTGG+TGG 0.530641 3.2:-72340319 MS.gene77503:CDS
TCAAACTTGAACTTCAAGCT+AGG 0.546383 3.2:+72340244 None:intergenic
TTCAAACACCTTAGCTTCAG+GGG 0.554009 3.2:+72340531 None:intergenic
ATAATTACCGCTGGAAATAG+GGG 0.565206 3.2:+72340474 None:intergenic
GGTTGTCTTCCATACGTTGG+TGG 0.578596 3.2:-72341227 MS.gene77503:CDS
GCTGGTTGTCTTCCATACGT+TGG 0.583066 3.2:-72341230 MS.gene77503:CDS
TTGGCACTACCACCAACGTA+TGG 0.586024 3.2:+72341218 None:intergenic
TGGAAGCTGTACAGCAAGAG+TGG 0.593369 3.2:+72341079 None:intergenic
TCAACTTGATACCAAGTTTG+GGG 0.608436 3.2:+72341105 None:intergenic
ATGTTTAAGGTTCTGCTGAG+AGG 0.614875 3.2:-72340380 MS.gene77503:intron
AGAGTGGTGGTGGATTGGGA+AGG 0.615185 3.2:-72340332 MS.gene77503:CDS
GGGATCAAGCTCAAGTTCTG+AGG 0.620498 3.2:-72340358 MS.gene77503:CDS
GGTCATTGCGATCTTTGATG+AGG 0.621163 3.2:+72341126 None:intergenic
GTTTAAGGTTCTGCTGAGAG+GGG 0.633464 3.2:-72340378 MS.gene77503:intron
AAGTACAACAATCTATAGTG+GGG 0.642782 3.2:+72341192 None:intergenic
AGTTCCTCCCCTATTTCCAG+CGG 0.660557 3.2:-72340481 MS.gene77503:intron
ATTACCGCTGGAAATAGGGG+AGG 0.669828 3.2:+72340477 None:intergenic
TCAAGTTCTGAGGAAAAGAG+TGG 0.677014 3.2:-72340348 MS.gene77503:CDS
AGTACAACAATCTATAGTGG+GGG 0.691483 3.2:+72341193 None:intergenic
AGTTCTGAGGAAAAGAGTGG+TGG 0.693229 3.2:-72340345 MS.gene77503:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGAGAATTTTTTTTTGT+TGG - chr3.2:72340921-72340940 MS.gene77503:intron 15.0%
!! AAAATTATAATAATTACCGC+TGG + chr3.2:72341139-72341158 None:intergenic 20.0%
!! ATATAAATAAGGCTTAATCA+TGG - chr3.2:72340804-72340823 MS.gene77503:intron 20.0%
!!! TAGTTTGCTTTATGTATTAA+GGG - chr3.2:72340625-72340644 MS.gene77503:intron 20.0%
!!! TTAGTTTGCTTTATGTATTA+AGG - chr3.2:72340624-72340643 MS.gene77503:intron 20.0%
! AAGTGAGATATGAAAAGAAA+TGG + chr3.2:72341331-72341350 None:intergenic 25.0%
! ACACATTCAGTTATATTAGA+TGG + chr3.2:72340563-72340582 None:intergenic 25.0%
! AGAACCTTAAACATTATTAG+AGG + chr3.2:72341215-72341234 None:intergenic 25.0%
! ATAATCTTCAAAAGTAAGTG+AGG - chr3.2:72340835-72340854 MS.gene77503:intron 25.0%
! TCTAATATAACTGAATGTGT+AGG - chr3.2:72340563-72340582 MS.gene77503:intron 25.0%
!!! CACTTACTTTTGAAGATTAT+TGG + chr3.2:72340835-72340854 None:intergenic 25.0%
!!! GAGAATTTTTTTTTGTTGGT+CGG - chr3.2:72340925-72340944 MS.gene77503:intron 25.0%
!!! TGCATTATTATTTCTACTGT+TGG - chr3.2:72340283-72340302 MS.gene77503:CDS 25.0%
AAGTACAACAATCTATAGTG+GGG + chr3.2:72340412-72340431 None:intergenic 30.0%
AATAATTACCGCTGGAAATA+GGG + chr3.2:72341131-72341150 None:intergenic 30.0%
ATTCAACTTGATACCAAGTT+TGG + chr3.2:72340501-72340520 None:intergenic 30.0%
CATATGTATATAGTCCAATC+AGG + chr3.2:72340651-72340670 None:intergenic 30.0%
GAAGTACAACAATCTATAGT+GGG + chr3.2:72340413-72340432 None:intergenic 30.0%
TAATAATTACCGCTGGAAAT+AGG + chr3.2:72341132-72341151 None:intergenic 30.0%
TGTGCCTCTAATAATGTTTA+AGG - chr3.2:72341208-72341227 MS.gene77503:CDS 30.0%
TTCAACTTGATACCAAGTTT+GGG + chr3.2:72340500-72340519 None:intergenic 30.0%
! CTTATCAAGGACTTGTTTAA+GGG + chr3.2:72340435-72340454 None:intergenic 30.0%
! TAATGCAGTTACACTTTTGT+TGG + chr3.2:72340270-72340289 None:intergenic 30.0%
! TCTTATCAAGGACTTGTTTA+AGG + chr3.2:72340436-72340455 None:intergenic 30.0%
!! ACATTCCATTGAGTTGAATT+AGG + chr3.2:72340997-72341016 None:intergenic 30.0%
!! ACTTTTGAAGATTATTGGAC+CGG + chr3.2:72340830-72340849 None:intergenic 30.0%
!! TTATTATTTCTACTGTTGGC+TGG - chr3.2:72340287-72340306 MS.gene77503:CDS 30.0%
!!! TGCATTTTTTGGTCTTATCA+AGG + chr3.2:72340448-72340467 None:intergenic 30.0%
AAATAAGGCTTAATCATGGC+CGG - chr3.2:72340808-72340827 MS.gene77503:intron 35.0%
AATGTCTCATGTGACAAAAG+AGG + chr3.2:72340887-72340906 None:intergenic 35.0%
AGTACAACAATCTATAGTGG+GGG + chr3.2:72340411-72340430 None:intergenic 35.0%
ATAATTACCGCTGGAAATAG+GGG + chr3.2:72341130-72341149 None:intergenic 35.0%
GAGGAGACATATATTGAACT+TGG + chr3.2:72340868-72340887 None:intergenic 35.0%
TCAACTTGATACCAAGTTTG+GGG + chr3.2:72340499-72340518 None:intergenic 35.0%
TTATGTATTAAGGGCCTGAT+TGG - chr3.2:72340634-72340653 MS.gene77503:intron 35.0%
! GGAAGTACAACAATCTATAG+TGG + chr3.2:72340414-72340433 None:intergenic 35.0%
!! GATAAACTTGTTACTCTTGC+TGG - chr3.2:72340353-72340372 MS.gene77503:CDS 35.0%
!!! GAGGATACAGATGCATTTTT+TGG + chr3.2:72340459-72340478 None:intergenic 35.0%
CTTCAAACACCTTAGCTTCA+GGG + chr3.2:72341074-72341093 None:intergenic 40.0%
GGTGACAAATGTAGAAGATC+TGG + chr3.2:72341045-72341064 None:intergenic 40.0%
TTCAAACACCTTAGCTTCAG+GGG + chr3.2:72341073-72341092 None:intergenic 40.0%
! ACAATCTATAGTGGGGGTAT+TGG + chr3.2:72340405-72340424 None:intergenic 40.0%
! ATGTTTAAGGTTCTGCTGAG+AGG - chr3.2:72341221-72341240 MS.gene77503:CDS 40.0%
! TGTTTAAGGTTCTGCTGAGA+GGG - chr3.2:72341222-72341241 MS.gene77503:CDS 40.0%
!! ATTGAGTTGAATTAGGCACG+GGG + chr3.2:72340990-72341009 None:intergenic 40.0%
!! CATTGAGTTGAATTAGGCAC+GGG + chr3.2:72340991-72341010 None:intergenic 40.0%
!! CCATTGAGTTGAATTAGGCA+CGG + chr3.2:72340992-72341011 None:intergenic 40.0%
!! TAGTAGCTTTACTTTTGCGC+CGG - chr3.2:72340964-72340983 MS.gene77503:intron 40.0%
!! TCAAGTTCTGAGGAAAAGAG+TGG - chr3.2:72341253-72341272 MS.gene77503:CDS 40.0%
ACCTAAATCCCCTGAAGCTA+AGG - chr3.2:72341062-72341081 MS.gene77503:CDS 45.0%
ACCTTAGCTTCAGGGGATTT+AGG + chr3.2:72341066-72341085 None:intergenic 45.0%
CCGTGCCTAATTCAACTCAA+TGG - chr3.2:72340989-72341008 MS.gene77503:intron 45.0%
CCTTCAAACACCTTAGCTTC+AGG + chr3.2:72341075-72341094 None:intergenic 45.0%
GATGGTATGTGACAAGCGTT+TGG + chr3.2:72340545-72340564 None:intergenic 45.0%
GGTCATTGCGATCTTTGATG+AGG + chr3.2:72340478-72340497 None:intergenic 45.0%
! AGGGGAGGAACTGTTTGTTT+TGG + chr3.2:72341112-72341131 None:intergenic 45.0%
! CCTGAAGCTAAGGTGTTTGA+AGG - chr3.2:72341072-72341091 MS.gene77503:CDS 45.0%
! GCTAAGGTGTTTGAAGGACT+TGG - chr3.2:72341078-72341097 MS.gene77503:CDS 45.0%
! GTTTAAGGTTCTGCTGAGAG+GGG - chr3.2:72341223-72341242 MS.gene77503:CDS 45.0%
!! AGTTCTGAGGAAAAGAGTGG+TGG - chr3.2:72341256-72341275 MS.gene77503:CDS 45.0%
!! GAAAAGAGTGGTGGTGGATT+GGG - chr3.2:72341265-72341284 MS.gene77503:CDS 45.0%
!! GCTACTAAAGCCGTCGTTTT+TGG + chr3.2:72340951-72340970 None:intergenic 45.0%
!! TTAAAATGTTTATATAAATA+AGG - chr3.2:72340793-72340812 MS.gene77503:intron 5.0%
AGTTCCTCCCCTATTTCCAG+CGG - chr3.2:72341120-72341139 MS.gene77503:CDS 50.0%
ATTACCGCTGGAAATAGGGG+AGG + chr3.2:72341127-72341146 None:intergenic 50.0%
GATCGCAATGACCCCAAACT+TGG - chr3.2:72340485-72340504 MS.gene77503:CDS 50.0%
GCTGGTTGTCTTCCATACGT+TGG - chr3.2:72340371-72340390 MS.gene77503:CDS 50.0%
GGGATCAAGCTCAAGTTCTG+AGG - chr3.2:72341243-72341262 MS.gene77503:CDS 50.0%
GGTTGTCTTCCATACGTTGG+TGG - chr3.2:72340374-72340393 MS.gene77503:CDS 50.0%
TCTGAGGAAAAGAGTGGTGG+TGG - chr3.2:72341259-72341278 MS.gene77503:CDS 50.0%
TGGAAGCTGTACAGCAAGAG+TGG + chr3.2:72340525-72340544 None:intergenic 50.0%
TGTTGGTCGGCCAAAAACGA+CGG - chr3.2:72340938-72340957 MS.gene77503:intron 50.0%
TTGGCACTACCACCAACGTA+TGG + chr3.2:72340386-72340405 None:intergenic 50.0%
!! GGAAAAGAGTGGTGGTGGAT+TGG - chr3.2:72341264-72341283 MS.gene77503:CDS 50.0%
AGTTGAATTAGGCACGGGGC+CGG + chr3.2:72340986-72341005 None:intergenic 55.0%
!! AGAGTGGTGGTGGATTGGGA+AGG - chr3.2:72341269-72341288 MS.gene77503:CDS 55.0%
ATTGGGAAGGAGGTGGCTGG+TGG - chr3.2:72341282-72341301 MS.gene77503:CDS 60.0%
! TGGATTGGGAAGGAGGTGGC+TGG - chr3.2:72341279-72341298 MS.gene77503:CDS 60.0%
!! GTGGTGGATTGGGAAGGAGG+TGG - chr3.2:72341275-72341294 MS.gene77503:CDS 60.0%
!! GTGGTGGTGGATTGGGAAGG+AGG - chr3.2:72341272-72341291 MS.gene77503:CDS 60.0%
AAGGAGGTGGCTGGTGGCAG+AGG - chr3.2:72341288-72341307 MS.gene77503:CDS 65.0%
GAGGTGGCTGGTGGCAGAGG+TGG - chr3.2:72341291-72341310 MS.gene77503:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 72340257 72341366 72340257 ID=MS.gene77503
chr3.2 mRNA 72340257 72341366 72340257 ID=MS.gene77503.t1;Parent=MS.gene77503
chr3.2 exon 72341045 72341366 72341045 ID=MS.gene77503.t1.exon1;Parent=MS.gene77503.t1
chr3.2 CDS 72341045 72341366 72341045 ID=cds.MS.gene77503.t1;Parent=MS.gene77503.t1
chr3.2 exon 72340482 72340577 72340482 ID=MS.gene77503.t1.exon2;Parent=MS.gene77503.t1
chr3.2 CDS 72340482 72340577 72340482 ID=cds.MS.gene77503.t1;Parent=MS.gene77503.t1
chr3.2 exon 72340257 72340393 72340257 ID=MS.gene77503.t1.exon3;Parent=MS.gene77503.t1
chr3.2 CDS 72340257 72340393 72340257 ID=cds.MS.gene77503.t1;Parent=MS.gene77503.t1
Gene Sequence

>MS.gene77503

ATGATGGTTTCCAACAAAAGTGTAACTGCATTATTATTTCTACTGTTGGCTGGTTTTGTGAGCTCAGATTTTACAAAAGACAGAAACGATTGTGCTGATAAACTTGTTACTCTTGCTGGTTGTCTTCCATACGTTGGTGGTAGTGCCAATACCCCCACTATAGATTGTTGTACTTCCCTTAAACAAGTCCTTGATAAGACCAAAAAATGCATCTGTATCCTCATCAAAGATCGCAATGACCCCAAACTTGGTATCAAGTTGAATGCCACTCTTGCTGTACAGCTTCCAAACGCTTGTCACATACCATCTAATATAACTGAATGTGTAGGCAAGTATATTTTTCTCATGATCTTATCTAGCTATTAGTTTAGTTTGCTTTATGTATTAAGGGCCTGATTGGACTATATACATATGCTGTTTAAGCTGATTTTCATAAACTTTTCAAATAGCTTATGAAAACAACTTATAGATTATACAGAAATAATTTGACTTTATTATACATAAATGATATACGTTATAAATGACATAAGTGTTTATTAAAATGTTTATATAAATAAGGCTTAATCATGGCCGGTCCAATAATCTTCAAAAGTAAGTGAGGCATAAAGCCAAGTTCAATATATGTCTCCTCTTTTGTCACATGAGACATTTTTCTTGTAAAATCTAATGAGAATTTTTTTTTGTTGGTCGGCCAAAAACGACGGCTTTAGTAGCTTTACTTTTGCGCCGGCCCCGTGCCTAATTCAACTCAATGGAATGTTTAATTTATACTTTTGTATTTTTTTCCAGATCTTCTACATTTGTCACCTAAATCCCCTGAAGCTAAGGTGTTTGAAGGACTTGGAAATTCTACCAAAACAAACAGTTCCTCCCCTATTTCCAGCGGTAATTATTATAATTTTAGTATTGTTATTGAGTAATTTTTGCGTGTATTGAAAATTGAAGCTAATTTGTGCCTCTAATAATGTTTAAGGTTCTGCTGAGAGGGGATCAAGCTCAAGTTCTGAGGAAAAGAGTGGTGGTGGATTGGGAAGGAGGTGGCTGGTGGCAGAGGTGGTTTGTGCTATTTTACCATTTCTTTTCATATCTCACTTCTTCATCCTAGCTTGA

Protein sequence

>MS.gene77503.t1

MMVSNKSVTALLFLLLAGFVSSDFTKDRNDCADKLVTLAGCLPYVGGSANTPTIDCCTSLKQVLDKTKKCICILIKDRNDPKLGIKLNATLAVQLPNACHIPSNITEYLLHLSPKSPEAKVFEGLGNSTKTNSSSPISSGSAERGSSSSSEEKSGGGLGRRWLVAEVVCAILPFLFISHFFILA