AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75704


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75704.t1 MTR_3g083760 82.673 202 16 1 1 183 1 202 1.18e-94 273
MS.gene75704.t1 MTR_3g083760 87.413 143 16 1 1 141 1 143 6.48e-81 236
MS.gene75704.t1 MTR_8g085560 50.829 181 83 3 9 183 14 194 5.45e-66 201
MS.gene75704.t1 MTR_7g014880 42.222 180 93 3 8 176 12 191 3.99e-41 137
MS.gene75704.t1 MTR_8g446830 39.487 195 105 6 1 182 1 195 1.05e-36 126
MS.gene75704.t1 MTR_1g103450 31.333 150 79 4 12 161 15 140 7.10e-13 63.5
MS.gene75704.t1 MTR_1g103490 36.667 90 55 2 12 101 10 97 2.34e-12 62.0
MS.gene75704.t1 MTR_1g103470 39.189 74 42 2 28 101 25 95 1.66e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75704.t1 AT1G55260 43.258 178 93 4 8 181 53 226 6.91e-44 145
MS.gene75704.t1 AT1G55260 43.258 178 90 5 8 181 53 223 2.21e-43 144
MS.gene75704.t1 AT2G44300 39.796 196 96 5 7 183 11 203 1.38e-40 136
MS.gene75704.t1 AT2G44290 38.579 197 96 4 9 183 11 204 1.60e-40 136
MS.gene75704.t1 AT3G58550 38.926 149 84 4 4 145 9 157 6.51e-30 108
MS.gene75704.t1 AT1G03103 31.915 94 64 0 8 101 2 95 1.91e-14 68.2
MS.gene75704.t1 AT5G09370 37.736 106 61 2 24 127 21 123 3.16e-14 66.6
MS.gene75704.t1 AT5G09370 37.600 125 64 5 24 138 21 141 3.74e-14 67.0
MS.gene75704.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene75704.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene75704.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene75704.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene75704.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene75704.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene75704.t1 AT5G09370 39.175 97 54 2 24 118 21 114 6.05e-14 66.6
MS.gene75704.t1 AT1G73890 39.189 74 45 0 31 104 30 103 7.41e-14 67.0
MS.gene75704.t1 AT3G22600 28.188 149 95 5 10 150 6 150 2.51e-12 62.4
MS.gene75704.t1 AT4G14815 30.000 90 62 1 12 101 7 95 4.42e-11 58.9

Find 51 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAATTACCGCTGGAAAT+AGG 0.158824 3.3:+70285781 None:intergenic
TCTTATCAAGGACTTGTTTA+AGG 0.173161 3.3:+70286473 None:intergenic
ACCTTAGCTTCAGGGGATTT+AGG 0.238114 3.3:+70285847 None:intergenic
AATAATTACCGCTGGAAATA+GGG 0.274317 3.3:+70285782 None:intergenic
AAGGAGGTGGCTGGTGGCAG+AGG 0.280788 3.3:-70285622 MS.gene75704:CDS
ATTCAACTTGATACCAAGTT+TGG 0.282616 3.3:+70286408 None:intergenic
GATAAACTTGTTACTCTTGC+TGG 0.316761 3.3:-70286553 MS.gene75704:CDS
CCTTCAAACACCTTAGCTTC+AGG 0.355634 3.3:+70285838 None:intergenic
TTCAACTTGATACCAAGTTT+GGG 0.358716 3.3:+70286409 None:intergenic
ACACATTCAGTTATATTAGA+TGG 0.359046 3.3:+70286346 None:intergenic
TGGATTGGGAAGGAGGTGGC+TGG 0.375917 3.3:-70285631 MS.gene75704:CDS
AGGGGAGGAACTGTTTGTTT+TGG 0.377595 3.3:+70285801 None:intergenic
GAAAAGAGTGGTGGTGGATT+GGG 0.380896 3.3:-70285645 MS.gene75704:CDS
TGCATTATTATTTCTACTGT+TGG 0.388007 3.3:-70286623 MS.gene75704:CDS
TTATTATTTCTACTGTTGGC+TGG 0.403176 3.3:-70286619 MS.gene75704:CDS
CTTATCAAGGACTTGTTTAA+GGG 0.407090 3.3:+70286474 None:intergenic
GATGGTATGTGACAAGCGTT+TGG 0.413443 3.3:+70286364 None:intergenic
GGAAAAGAGTGGTGGTGGAT+TGG 0.435272 3.3:-70285646 MS.gene75704:CDS
GCTAAGGTGTTTGAAGGACT+TGG 0.437073 3.3:-70285832 MS.gene75704:CDS
GAGGTGGCTGGTGGCAGAGG+TGG 0.447551 3.3:-70285619 MS.gene75704:CDS
AAGTGAGATATGAAAAGAAA+TGG 0.450601 3.3:+70285582 None:intergenic
GGAAGTACAACAATCTATAG+TGG 0.456166 3.3:+70286495 None:intergenic
CTTCAAACACCTTAGCTTCA+GGG 0.472455 3.3:+70285839 None:intergenic
ACCTAAATCCCCTGAAGCTA+AGG 0.474094 3.3:-70285848 MS.gene75704:CDS
CCTGAAGCTAAGGTGTTTGA+AGG 0.476875 3.3:-70285838 MS.gene75704:CDS
GATCGCAATGACCCCAAACT+TGG 0.492547 3.3:-70286421 MS.gene75704:CDS
GTGGTGGATTGGGAAGGAGG+TGG 0.495091 3.3:-70285635 MS.gene75704:CDS
GTGGTGGTGGATTGGGAAGG+AGG 0.496077 3.3:-70285638 MS.gene75704:CDS
TCTGAGGAAAAGAGTGGTGG+TGG 0.499326 3.3:-70285651 MS.gene75704:CDS
TGTTTAAGGTTCTGCTGAGA+GGG 0.503753 3.3:-70285688 MS.gene75704:intron
GAAGTACAACAATCTATAGT+GGG 0.507467 3.3:+70286496 None:intergenic
ATTGGGAAGGAGGTGGCTGG+TGG 0.530641 3.3:-70285628 MS.gene75704:CDS
TCAAACTTGAACTTCAAGCT+AGG 0.546383 3.3:+70285553 None:intergenic
TTCAAACACCTTAGCTTCAG+GGG 0.554009 3.3:+70285840 None:intergenic
ATAATTACCGCTGGAAATAG+GGG 0.565206 3.3:+70285783 None:intergenic
GGTTGTCTTCCATACGTTGG+TGG 0.578596 3.3:-70286532 MS.gene75704:CDS
GCTGGTTGTCTTCCATACGT+TGG 0.583066 3.3:-70286535 MS.gene75704:CDS
TTGGCACTACCACCAACGTA+TGG 0.586024 3.3:+70286523 None:intergenic
TGGAAGCTGTACAGCAAGAG+TGG 0.593369 3.3:+70286384 None:intergenic
TCAACTTGATACCAAGTTTG+GGG 0.608436 3.3:+70286410 None:intergenic
ATGTTTAAGGTTCTGCTGAG+AGG 0.614875 3.3:-70285689 MS.gene75704:intron
AGAGTGGTGGTGGATTGGGA+AGG 0.615185 3.3:-70285641 MS.gene75704:CDS
GGGATCAAGCTCAAGTTCTG+AGG 0.620498 3.3:-70285667 MS.gene75704:CDS
GGTCATTGCGATCTTTGATG+AGG 0.621163 3.3:+70286431 None:intergenic
GTTTAAGGTTCTGCTGAGAG+GGG 0.633464 3.3:-70285687 MS.gene75704:intron
AAGTACAACAATCTATAGTG+GGG 0.642782 3.3:+70286497 None:intergenic
AGTTCCTCCCCTATTTCCAG+CGG 0.660557 3.3:-70285790 MS.gene75704:intron
ATTACCGCTGGAAATAGGGG+AGG 0.669828 3.3:+70285786 None:intergenic
TCAAGTTCTGAGGAAAAGAG+TGG 0.677014 3.3:-70285657 MS.gene75704:CDS
AGTACAACAATCTATAGTGG+GGG 0.691483 3.3:+70286498 None:intergenic
AGTTCTGAGGAAAAGAGTGG+TGG 0.693229 3.3:-70285654 MS.gene75704:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGAGAATTTTTTTTTGT+TGG - chr3.3:70286226-70286245 MS.gene75704:intron 15.0%
!! AAAATTATAATAATTACCGC+TGG + chr3.3:70286444-70286463 None:intergenic 20.0%
!! ATATAAATAAGGCTTAATCA+TGG - chr3.3:70286109-70286128 MS.gene75704:intron 20.0%
!!! TAGTTTGCTTTATGTATTAA+GGG - chr3.3:70285934-70285953 MS.gene75704:intron 20.0%
!!! TTAGTTTGCTTTATGTATTA+AGG - chr3.3:70285933-70285952 MS.gene75704:intron 20.0%
! AAGTGAGATATGAAAAGAAA+TGG + chr3.3:70286636-70286655 None:intergenic 25.0%
! ACACATTCAGTTATATTAGA+TGG + chr3.3:70285872-70285891 None:intergenic 25.0%
! AGAACCTTAAACATTATTAG+AGG + chr3.3:70286520-70286539 None:intergenic 25.0%
! ATAATCTTCAAAAGTAAGTG+AGG - chr3.3:70286140-70286159 MS.gene75704:intron 25.0%
! TCTAATATAACTGAATGTGT+AGG - chr3.3:70285872-70285891 MS.gene75704:intron 25.0%
!!! CACTTACTTTTGAAGATTAT+TGG + chr3.3:70286140-70286159 None:intergenic 25.0%
!!! GAGAATTTTTTTTTGTTGGT+CGG - chr3.3:70286230-70286249 MS.gene75704:intron 25.0%
!!! TGCATTATTATTTCTACTGT+TGG - chr3.3:70285592-70285611 MS.gene75704:CDS 25.0%
AAGTACAACAATCTATAGTG+GGG + chr3.3:70285721-70285740 None:intergenic 30.0%
AATAATTACCGCTGGAAATA+GGG + chr3.3:70286436-70286455 None:intergenic 30.0%
ATTCAACTTGATACCAAGTT+TGG + chr3.3:70285810-70285829 None:intergenic 30.0%
CATATGTATATAGTCCAATC+AGG + chr3.3:70285960-70285979 None:intergenic 30.0%
GAAGTACAACAATCTATAGT+GGG + chr3.3:70285722-70285741 None:intergenic 30.0%
TAATAATTACCGCTGGAAAT+AGG + chr3.3:70286437-70286456 None:intergenic 30.0%
TGTGCCTCTAATAATGTTTA+AGG - chr3.3:70286513-70286532 MS.gene75704:CDS 30.0%
TTCAACTTGATACCAAGTTT+GGG + chr3.3:70285809-70285828 None:intergenic 30.0%
! CTTATCAAGGACTTGTTTAA+GGG + chr3.3:70285744-70285763 None:intergenic 30.0%
! TAATGCAGTTACACTTTTGT+TGG + chr3.3:70285579-70285598 None:intergenic 30.0%
! TCTTATCAAGGACTTGTTTA+AGG + chr3.3:70285745-70285764 None:intergenic 30.0%
!! ACATTCCATTGAGTTGAATT+AGG + chr3.3:70286302-70286321 None:intergenic 30.0%
!! ACTTTTGAAGATTATTGGAC+CGG + chr3.3:70286135-70286154 None:intergenic 30.0%
!! TTATTATTTCTACTGTTGGC+TGG - chr3.3:70285596-70285615 MS.gene75704:CDS 30.0%
!!! TGCATTTTTTGGTCTTATCA+AGG + chr3.3:70285757-70285776 None:intergenic 30.0%
AAATAAGGCTTAATCATGGC+CGG - chr3.3:70286113-70286132 MS.gene75704:intron 35.0%
AATGTCTCATGTGACAAAAG+AGG + chr3.3:70286192-70286211 None:intergenic 35.0%
AGTACAACAATCTATAGTGG+GGG + chr3.3:70285720-70285739 None:intergenic 35.0%
ATAATTACCGCTGGAAATAG+GGG + chr3.3:70286435-70286454 None:intergenic 35.0%
TCAACTTGATACCAAGTTTG+GGG + chr3.3:70285808-70285827 None:intergenic 35.0%
TTATGTATTAAGGGCCTGAT+TGG - chr3.3:70285943-70285962 MS.gene75704:intron 35.0%
! GGAAGTACAACAATCTATAG+TGG + chr3.3:70285723-70285742 None:intergenic 35.0%
!! GATAAACTTGTTACTCTTGC+TGG - chr3.3:70285662-70285681 MS.gene75704:CDS 35.0%
!!! GAGGATACAGATGCATTTTT+TGG + chr3.3:70285768-70285787 None:intergenic 35.0%
CTTCAAACACCTTAGCTTCA+GGG + chr3.3:70286379-70286398 None:intergenic 40.0%
GGTGACAAATGTAGAAGATC+TGG + chr3.3:70286350-70286369 None:intergenic 40.0%
TTCAAACACCTTAGCTTCAG+GGG + chr3.3:70286378-70286397 None:intergenic 40.0%
! ACAATCTATAGTGGGGGTAT+TGG + chr3.3:70285714-70285733 None:intergenic 40.0%
! ATGTTTAAGGTTCTGCTGAG+AGG - chr3.3:70286526-70286545 MS.gene75704:CDS 40.0%
! TGTTTAAGGTTCTGCTGAGA+GGG - chr3.3:70286527-70286546 MS.gene75704:CDS 40.0%
!! ATTGAGTTGAATTAGGCACG+GGG + chr3.3:70286295-70286314 None:intergenic 40.0%
!! CATTGAGTTGAATTAGGCAC+GGG + chr3.3:70286296-70286315 None:intergenic 40.0%
!! CCATTGAGTTGAATTAGGCA+CGG + chr3.3:70286297-70286316 None:intergenic 40.0%
!! TAGTAGCTTTACTTTTGCGC+CGG - chr3.3:70286269-70286288 MS.gene75704:intron 40.0%
!! TCAAGTTCTGAGGAAAAGAG+TGG - chr3.3:70286558-70286577 MS.gene75704:CDS 40.0%
ACCTAAATCCCCTGAAGCTA+AGG - chr3.3:70286367-70286386 MS.gene75704:CDS 45.0%
ACCTTAGCTTCAGGGGATTT+AGG + chr3.3:70286371-70286390 None:intergenic 45.0%
CCGTGCCTAATTCAACTCAA+TGG - chr3.3:70286294-70286313 MS.gene75704:intron 45.0%
CCTTCAAACACCTTAGCTTC+AGG + chr3.3:70286380-70286399 None:intergenic 45.0%
GATGGTATGTGACAAGCGTT+TGG + chr3.3:70285854-70285873 None:intergenic 45.0%
GGTCATTGCGATCTTTGATG+AGG + chr3.3:70285787-70285806 None:intergenic 45.0%
! AGGGGAGGAACTGTTTGTTT+TGG + chr3.3:70286417-70286436 None:intergenic 45.0%
! CCTGAAGCTAAGGTGTTTGA+AGG - chr3.3:70286377-70286396 MS.gene75704:CDS 45.0%
! GCTAAGGTGTTTGAAGGACT+TGG - chr3.3:70286383-70286402 MS.gene75704:CDS 45.0%
! GTTTAAGGTTCTGCTGAGAG+GGG - chr3.3:70286528-70286547 MS.gene75704:CDS 45.0%
!! AGTTCTGAGGAAAAGAGTGG+TGG - chr3.3:70286561-70286580 MS.gene75704:CDS 45.0%
!! GAAAAGAGTGGTGGTGGATT+GGG - chr3.3:70286570-70286589 MS.gene75704:CDS 45.0%
!! GCTACTAAAGCCGTCGTTTT+TGG + chr3.3:70286256-70286275 None:intergenic 45.0%
!! TTAAAATGTTTATATAAATA+AGG - chr3.3:70286098-70286117 MS.gene75704:intron 5.0%
AGTTCCTCCCCTATTTCCAG+CGG - chr3.3:70286425-70286444 MS.gene75704:CDS 50.0%
ATTACCGCTGGAAATAGGGG+AGG + chr3.3:70286432-70286451 None:intergenic 50.0%
GATCGCAATGACCCCAAACT+TGG - chr3.3:70285794-70285813 MS.gene75704:CDS 50.0%
GCTGGTTGTCTTCCATACGT+TGG - chr3.3:70285680-70285699 MS.gene75704:CDS 50.0%
GGGATCAAGCTCAAGTTCTG+AGG - chr3.3:70286548-70286567 MS.gene75704:CDS 50.0%
GGTTGTCTTCCATACGTTGG+TGG - chr3.3:70285683-70285702 MS.gene75704:CDS 50.0%
TCTGAGGAAAAGAGTGGTGG+TGG - chr3.3:70286564-70286583 MS.gene75704:CDS 50.0%
TGGAAGCTGTACAGCAAGAG+TGG + chr3.3:70285834-70285853 None:intergenic 50.0%
TGTTGGTCGGCCAAAAACGA+CGG - chr3.3:70286243-70286262 MS.gene75704:intron 50.0%
TTGGCACTACCACCAACGTA+TGG + chr3.3:70285695-70285714 None:intergenic 50.0%
!! GGAAAAGAGTGGTGGTGGAT+TGG - chr3.3:70286569-70286588 MS.gene75704:CDS 50.0%
AGTTGAATTAGGCACGGGGC+CGG + chr3.3:70286291-70286310 None:intergenic 55.0%
!! AGAGTGGTGGTGGATTGGGA+AGG - chr3.3:70286574-70286593 MS.gene75704:CDS 55.0%
ATTGGGAAGGAGGTGGCTGG+TGG - chr3.3:70286587-70286606 MS.gene75704:CDS 60.0%
! TGGATTGGGAAGGAGGTGGC+TGG - chr3.3:70286584-70286603 MS.gene75704:CDS 60.0%
!! GTGGTGGATTGGGAAGGAGG+TGG - chr3.3:70286580-70286599 MS.gene75704:CDS 60.0%
!! GTGGTGGTGGATTGGGAAGG+AGG - chr3.3:70286577-70286596 MS.gene75704:CDS 60.0%
AAGGAGGTGGCTGGTGGCAG+AGG - chr3.3:70286593-70286612 MS.gene75704:CDS 65.0%
GAGGTGGCTGGTGGCAGAGG+TGG - chr3.3:70286596-70286615 MS.gene75704:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 70285566 70286671 70285566 ID=MS.gene75704
chr3.3 mRNA 70285566 70286671 70285566 ID=MS.gene75704.t1;Parent=MS.gene75704
chr3.3 exon 70286350 70286671 70286350 ID=MS.gene75704.t1.exon1;Parent=MS.gene75704.t1
chr3.3 CDS 70286350 70286671 70286350 ID=cds.MS.gene75704.t1;Parent=MS.gene75704.t1
chr3.3 exon 70285791 70285886 70285791 ID=MS.gene75704.t1.exon2;Parent=MS.gene75704.t1
chr3.3 CDS 70285791 70285886 70285791 ID=cds.MS.gene75704.t1;Parent=MS.gene75704.t1
chr3.3 exon 70285566 70285702 70285566 ID=MS.gene75704.t1.exon3;Parent=MS.gene75704.t1
chr3.3 CDS 70285566 70285702 70285566 ID=cds.MS.gene75704.t1;Parent=MS.gene75704.t1
Gene Sequence

>MS.gene75704

ATGATGGTTTCCAACAAAAGTGTAACTGCATTATTATTTCTACTGTTGGCTGGTTTTGTGAGCTCAGATTTTACAAAAGACAGAAACGATTGTGCTGATAAACTTGTTACTCTTGCTGGTTGTCTTCCATACGTTGGTGGTAGTGCCAATACCCCCACTATAGATTGTTGTACTTCCCTTAAACAAGTCCTTGATAAGACCAAAAAATGCATCTGTATCCTCATCAAAGATCGCAATGACCCCAAACTTGGTATCAAGTTGAATGCCACTCTTGCTGTACAGCTTCCAAACGCTTGTCACATACCATCTAATATAACTGAATGTGTAGGCAAGTATATTTTTCTCATGATCTTATCTAGCTATTAGTTTAGTTTGCTTTATGTATTAAGGGCCTGATTGGACTATATACATATGCTGTTTAAGCTGATTTTCATAAACTTTTCAAATAGCTTATGAAAACAACTTATAGATTATACAGAAATAATTTGACTTTATTATACATAAATGATATACGCTATAATATAAGTGTTTATTAAAATGTTTATATAAATAAGGCTTAATCATGGCCGGTCCAATAATCTTCAAAAGTAAGTGAGGCATAAAGCGAAGTTCAATATATGTCTCCTCTTTTGTCACATGAGACATTTTTCTTGTAAAATCTAATGAGAATTTTTTTTTGTTGGTCGGCCAAAAACGACGGCTTTAGTAGCTTTACTTTTGCGCCGGCCCCGTGCCTAATTCAACTCAATGGAATGTTTAATTTATACTTTTGTATTTTTTTCCAGATCTTCTACATTTGTCACCTAAATCCCCTGAAGCTAAGGTGTTTGAAGGACTTGGAAATTCTACCAAAACAAACAGTTCCTCCCCTATTTCCAGCGGTAATTATTATAATTTTAGTATTGTTATTGAGTAATTTTTGCGTGTATTGAAAATTGAAGCTAATTTGTGCCTCTAATAATGTTTAAGGTTCTGCTGAGAGGGGATCAAGCTCAAGTTCTGAGGAAAAGAGTGGTGGTGGATTGGGAAGGAGGTGGCTGGTGGCAGAGGTGGTTTGTGCTATTTTACCATTTCTTTTCATATCTCACTTCTTCATCCTAGCTTGA

Protein sequence

>MS.gene75704.t1

MMVSNKSVTALLFLLLAGFVSSDFTKDRNDCADKLVTLAGCLPYVGGSANTPTIDCCTSLKQVLDKTKKCICILIKDRNDPKLGIKLNATLAVQLPNACHIPSNITEYLLHLSPKSPEAKVFEGLGNSTKTNSSSPISSGSAERGSSSSSEEKSGGGLGRRWLVAEVVCAILPFLFISHFFILA