AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77500.t1 MTR_3g083760 82.673 202 16 1 1 183 1 202 1.18e-94 273
MS.gene77500.t1 MTR_3g083760 87.413 143 16 1 1 141 1 143 6.48e-81 236
MS.gene77500.t1 MTR_8g085560 50.829 181 83 3 9 183 14 194 5.45e-66 201
MS.gene77500.t1 MTR_7g014880 42.222 180 93 3 8 176 12 191 3.99e-41 137
MS.gene77500.t1 MTR_8g446830 39.487 195 105 6 1 182 1 195 1.05e-36 126
MS.gene77500.t1 MTR_1g103450 31.333 150 79 4 12 161 15 140 7.10e-13 63.5
MS.gene77500.t1 MTR_1g103490 36.667 90 55 2 12 101 10 97 2.34e-12 62.0
MS.gene77500.t1 MTR_1g103470 39.189 74 42 2 28 101 25 95 1.66e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77500.t1 AT1G55260 43.258 178 93 4 8 181 53 226 6.91e-44 145
MS.gene77500.t1 AT1G55260 43.258 178 90 5 8 181 53 223 2.21e-43 144
MS.gene77500.t1 AT2G44300 39.796 196 96 5 7 183 11 203 1.38e-40 136
MS.gene77500.t1 AT2G44290 38.579 197 96 4 9 183 11 204 1.60e-40 136
MS.gene77500.t1 AT3G58550 38.926 149 84 4 4 145 9 157 6.51e-30 108
MS.gene77500.t1 AT1G03103 31.915 94 64 0 8 101 2 95 1.91e-14 68.2
MS.gene77500.t1 AT5G09370 37.736 106 61 2 24 127 21 123 3.16e-14 66.6
MS.gene77500.t1 AT5G09370 37.600 125 64 5 24 138 21 141 3.74e-14 67.0
MS.gene77500.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77500.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77500.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77500.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77500.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77500.t1 AT2G48130 36.559 93 57 2 10 101 8 99 4.19e-14 67.4
MS.gene77500.t1 AT5G09370 39.175 97 54 2 24 118 21 114 6.05e-14 66.6
MS.gene77500.t1 AT1G73890 39.189 74 45 0 31 104 30 103 7.41e-14 67.0
MS.gene77500.t1 AT3G22600 28.188 149 95 5 10 150 6 150 2.51e-12 62.4
MS.gene77500.t1 AT4G14815 30.000 90 62 1 12 101 7 95 4.42e-11 58.9

Find 51 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAATTACCGCTGGAAAT+AGG 0.158824 3.2:+72315055 None:intergenic
TCTTATCAAGGACTTGTTTA+AGG 0.173161 3.2:+72315751 None:intergenic
ACCTTAGCTTCAGGGGATTT+AGG 0.238114 3.2:+72315121 None:intergenic
AATAATTACCGCTGGAAATA+GGG 0.274317 3.2:+72315056 None:intergenic
AAGGAGGTGGCTGGTGGCAG+AGG 0.280788 3.2:-72314896 MS.gene77500:CDS
ATTCAACTTGATACCAAGTT+TGG 0.282616 3.2:+72315686 None:intergenic
GATAAACTTGTTACTCTTGC+TGG 0.316761 3.2:-72315831 MS.gene77500:CDS
CCTTCAAACACCTTAGCTTC+AGG 0.355634 3.2:+72315112 None:intergenic
TTCAACTTGATACCAAGTTT+GGG 0.358716 3.2:+72315687 None:intergenic
ACACATTCAGTTATATTAGA+TGG 0.359046 3.2:+72315624 None:intergenic
TGGATTGGGAAGGAGGTGGC+TGG 0.375917 3.2:-72314905 MS.gene77500:CDS
AGGGGAGGAACTGTTTGTTT+TGG 0.377595 3.2:+72315075 None:intergenic
GAAAAGAGTGGTGGTGGATT+GGG 0.380896 3.2:-72314919 MS.gene77500:CDS
TGCATTATTATTTCTACTGT+TGG 0.388007 3.2:-72315901 MS.gene77500:CDS
TTATTATTTCTACTGTTGGC+TGG 0.403176 3.2:-72315897 MS.gene77500:CDS
CTTATCAAGGACTTGTTTAA+GGG 0.407090 3.2:+72315752 None:intergenic
GATGGTATGTGACAAGCGTT+TGG 0.413443 3.2:+72315642 None:intergenic
GGAAAAGAGTGGTGGTGGAT+TGG 0.435272 3.2:-72314920 MS.gene77500:CDS
GCTAAGGTGTTTGAAGGACT+TGG 0.437073 3.2:-72315106 MS.gene77500:CDS
GAGGTGGCTGGTGGCAGAGG+TGG 0.447551 3.2:-72314893 MS.gene77500:CDS
AAGTGAGATATGAAAAGAAA+TGG 0.450601 3.2:+72314856 None:intergenic
GGAAGTACAACAATCTATAG+TGG 0.456166 3.2:+72315773 None:intergenic
CTTCAAACACCTTAGCTTCA+GGG 0.472455 3.2:+72315113 None:intergenic
ACCTAAATCCCCTGAAGCTA+AGG 0.474094 3.2:-72315122 MS.gene77500:CDS
CCTGAAGCTAAGGTGTTTGA+AGG 0.476875 3.2:-72315112 MS.gene77500:CDS
GATCGCAATGACCCCAAACT+TGG 0.492547 3.2:-72315699 MS.gene77500:CDS
GTGGTGGATTGGGAAGGAGG+TGG 0.495091 3.2:-72314909 MS.gene77500:CDS
GTGGTGGTGGATTGGGAAGG+AGG 0.496077 3.2:-72314912 MS.gene77500:CDS
TCTGAGGAAAAGAGTGGTGG+TGG 0.499326 3.2:-72314925 MS.gene77500:CDS
TGTTTAAGGTTCTGCTGAGA+GGG 0.503753 3.2:-72314962 MS.gene77500:intron
GAAGTACAACAATCTATAGT+GGG 0.507467 3.2:+72315774 None:intergenic
ATTGGGAAGGAGGTGGCTGG+TGG 0.530641 3.2:-72314902 MS.gene77500:CDS
TCAAACTTGAACTTCAAGCT+AGG 0.546383 3.2:+72314827 None:intergenic
TTCAAACACCTTAGCTTCAG+GGG 0.554009 3.2:+72315114 None:intergenic
ATAATTACCGCTGGAAATAG+GGG 0.565206 3.2:+72315057 None:intergenic
GGTTGTCTTCCATACGTTGG+TGG 0.578596 3.2:-72315810 MS.gene77500:CDS
GCTGGTTGTCTTCCATACGT+TGG 0.583066 3.2:-72315813 MS.gene77500:CDS
TTGGCACTACCACCAACGTA+TGG 0.586024 3.2:+72315801 None:intergenic
TGGAAGCTGTACAGCAAGAG+TGG 0.593369 3.2:+72315662 None:intergenic
TCAACTTGATACCAAGTTTG+GGG 0.608436 3.2:+72315688 None:intergenic
ATGTTTAAGGTTCTGCTGAG+AGG 0.614875 3.2:-72314963 MS.gene77500:intron
AGAGTGGTGGTGGATTGGGA+AGG 0.615185 3.2:-72314915 MS.gene77500:CDS
GGGATCAAGCTCAAGTTCTG+AGG 0.620498 3.2:-72314941 MS.gene77500:CDS
GGTCATTGCGATCTTTGATG+AGG 0.621163 3.2:+72315709 None:intergenic
GTTTAAGGTTCTGCTGAGAG+GGG 0.633464 3.2:-72314961 MS.gene77500:intron
AAGTACAACAATCTATAGTG+GGG 0.642782 3.2:+72315775 None:intergenic
AGTTCCTCCCCTATTTCCAG+CGG 0.660557 3.2:-72315064 MS.gene77500:intron
ATTACCGCTGGAAATAGGGG+AGG 0.669828 3.2:+72315060 None:intergenic
TCAAGTTCTGAGGAAAAGAG+TGG 0.677014 3.2:-72314931 MS.gene77500:CDS
AGTACAACAATCTATAGTGG+GGG 0.691483 3.2:+72315776 None:intergenic
AGTTCTGAGGAAAAGAGTGG+TGG 0.693229 3.2:-72314928 MS.gene77500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGAGAATTTTTTTTTGT+TGG - chr3.2:72315504-72315523 MS.gene77500:intron 15.0%
!! AAAATTATAATAATTACCGC+TGG + chr3.2:72315722-72315741 None:intergenic 20.0%
!! ATATAAATAAGGCTTAATCA+TGG - chr3.2:72315387-72315406 MS.gene77500:intron 20.0%
!!! TAGTTTGCTTTATGTATTAA+GGG - chr3.2:72315208-72315227 MS.gene77500:intron 20.0%
!!! TTAGTTTGCTTTATGTATTA+AGG - chr3.2:72315207-72315226 MS.gene77500:intron 20.0%
! AAGTGAGATATGAAAAGAAA+TGG + chr3.2:72315914-72315933 None:intergenic 25.0%
! ACACATTCAGTTATATTAGA+TGG + chr3.2:72315146-72315165 None:intergenic 25.0%
! AGAACCTTAAACATTATTAG+AGG + chr3.2:72315798-72315817 None:intergenic 25.0%
! ATAATCTTCAAAAGTAAGTG+AGG - chr3.2:72315418-72315437 MS.gene77500:intron 25.0%
! TCTAATATAACTGAATGTGT+AGG - chr3.2:72315146-72315165 MS.gene77500:intron 25.0%
!!! CACTTACTTTTGAAGATTAT+TGG + chr3.2:72315418-72315437 None:intergenic 25.0%
!!! GAGAATTTTTTTTTGTTGGT+CGG - chr3.2:72315508-72315527 MS.gene77500:intron 25.0%
!!! TGCATTATTATTTCTACTGT+TGG - chr3.2:72314866-72314885 MS.gene77500:CDS 25.0%
AAGTACAACAATCTATAGTG+GGG + chr3.2:72314995-72315014 None:intergenic 30.0%
AATAATTACCGCTGGAAATA+GGG + chr3.2:72315714-72315733 None:intergenic 30.0%
ATTCAACTTGATACCAAGTT+TGG + chr3.2:72315084-72315103 None:intergenic 30.0%
CATATGTATATAGTCCAATC+AGG + chr3.2:72315234-72315253 None:intergenic 30.0%
GAAGTACAACAATCTATAGT+GGG + chr3.2:72314996-72315015 None:intergenic 30.0%
TAATAATTACCGCTGGAAAT+AGG + chr3.2:72315715-72315734 None:intergenic 30.0%
TGTGCCTCTAATAATGTTTA+AGG - chr3.2:72315791-72315810 MS.gene77500:CDS 30.0%
TTCAACTTGATACCAAGTTT+GGG + chr3.2:72315083-72315102 None:intergenic 30.0%
! CTTATCAAGGACTTGTTTAA+GGG + chr3.2:72315018-72315037 None:intergenic 30.0%
! TAATGCAGTTACACTTTTGT+TGG + chr3.2:72314853-72314872 None:intergenic 30.0%
! TCTTATCAAGGACTTGTTTA+AGG + chr3.2:72315019-72315038 None:intergenic 30.0%
!! ACATTCCATTGAGTTGAATT+AGG + chr3.2:72315580-72315599 None:intergenic 30.0%
!! ACTTTTGAAGATTATTGGAC+CGG + chr3.2:72315413-72315432 None:intergenic 30.0%
!! TTATTATTTCTACTGTTGGC+TGG - chr3.2:72314870-72314889 MS.gene77500:CDS 30.0%
!!! TGCATTTTTTGGTCTTATCA+AGG + chr3.2:72315031-72315050 None:intergenic 30.0%
AAATAAGGCTTAATCATGGC+CGG - chr3.2:72315391-72315410 MS.gene77500:intron 35.0%
AATGTCTCATGTGACAAAAG+AGG + chr3.2:72315470-72315489 None:intergenic 35.0%
AGTACAACAATCTATAGTGG+GGG + chr3.2:72314994-72315013 None:intergenic 35.0%
ATAATTACCGCTGGAAATAG+GGG + chr3.2:72315713-72315732 None:intergenic 35.0%
GAGGAGACATATATTGAACT+TGG + chr3.2:72315451-72315470 None:intergenic 35.0%
TCAACTTGATACCAAGTTTG+GGG + chr3.2:72315082-72315101 None:intergenic 35.0%
TTATGTATTAAGGGCCTGAT+TGG - chr3.2:72315217-72315236 MS.gene77500:intron 35.0%
! GGAAGTACAACAATCTATAG+TGG + chr3.2:72314997-72315016 None:intergenic 35.0%
!! GATAAACTTGTTACTCTTGC+TGG - chr3.2:72314936-72314955 MS.gene77500:CDS 35.0%
!!! GAGGATACAGATGCATTTTT+TGG + chr3.2:72315042-72315061 None:intergenic 35.0%
CTTCAAACACCTTAGCTTCA+GGG + chr3.2:72315657-72315676 None:intergenic 40.0%
GGTGACAAATGTAGAAGATC+TGG + chr3.2:72315628-72315647 None:intergenic 40.0%
TTCAAACACCTTAGCTTCAG+GGG + chr3.2:72315656-72315675 None:intergenic 40.0%
! ACAATCTATAGTGGGGGTAT+TGG + chr3.2:72314988-72315007 None:intergenic 40.0%
! ATGTTTAAGGTTCTGCTGAG+AGG - chr3.2:72315804-72315823 MS.gene77500:CDS 40.0%
! TGTTTAAGGTTCTGCTGAGA+GGG - chr3.2:72315805-72315824 MS.gene77500:CDS 40.0%
!! ATTGAGTTGAATTAGGCACG+GGG + chr3.2:72315573-72315592 None:intergenic 40.0%
!! CATTGAGTTGAATTAGGCAC+GGG + chr3.2:72315574-72315593 None:intergenic 40.0%
!! CCATTGAGTTGAATTAGGCA+CGG + chr3.2:72315575-72315594 None:intergenic 40.0%
!! TAGTAGCTTTACTTTTGCGC+CGG - chr3.2:72315547-72315566 MS.gene77500:intron 40.0%
!! TCAAGTTCTGAGGAAAAGAG+TGG - chr3.2:72315836-72315855 MS.gene77500:CDS 40.0%
ACCTAAATCCCCTGAAGCTA+AGG - chr3.2:72315645-72315664 MS.gene77500:CDS 45.0%
ACCTTAGCTTCAGGGGATTT+AGG + chr3.2:72315649-72315668 None:intergenic 45.0%
CCGTGCCTAATTCAACTCAA+TGG - chr3.2:72315572-72315591 MS.gene77500:intron 45.0%
CCTTCAAACACCTTAGCTTC+AGG + chr3.2:72315658-72315677 None:intergenic 45.0%
GATGGTATGTGACAAGCGTT+TGG + chr3.2:72315128-72315147 None:intergenic 45.0%
GGTCATTGCGATCTTTGATG+AGG + chr3.2:72315061-72315080 None:intergenic 45.0%
! AGGGGAGGAACTGTTTGTTT+TGG + chr3.2:72315695-72315714 None:intergenic 45.0%
! CCTGAAGCTAAGGTGTTTGA+AGG - chr3.2:72315655-72315674 MS.gene77500:CDS 45.0%
! GCTAAGGTGTTTGAAGGACT+TGG - chr3.2:72315661-72315680 MS.gene77500:CDS 45.0%
! GTTTAAGGTTCTGCTGAGAG+GGG - chr3.2:72315806-72315825 MS.gene77500:CDS 45.0%
!! AGTTCTGAGGAAAAGAGTGG+TGG - chr3.2:72315839-72315858 MS.gene77500:CDS 45.0%
!! GAAAAGAGTGGTGGTGGATT+GGG - chr3.2:72315848-72315867 MS.gene77500:CDS 45.0%
!! GCTACTAAAGCCGTCGTTTT+TGG + chr3.2:72315534-72315553 None:intergenic 45.0%
!! TTAAAATGTTTATATAAATA+AGG - chr3.2:72315376-72315395 MS.gene77500:intron 5.0%
AGTTCCTCCCCTATTTCCAG+CGG - chr3.2:72315703-72315722 MS.gene77500:CDS 50.0%
ATTACCGCTGGAAATAGGGG+AGG + chr3.2:72315710-72315729 None:intergenic 50.0%
GATCGCAATGACCCCAAACT+TGG - chr3.2:72315068-72315087 MS.gene77500:CDS 50.0%
GCTGGTTGTCTTCCATACGT+TGG - chr3.2:72314954-72314973 MS.gene77500:CDS 50.0%
GGGATCAAGCTCAAGTTCTG+AGG - chr3.2:72315826-72315845 MS.gene77500:CDS 50.0%
GGTTGTCTTCCATACGTTGG+TGG - chr3.2:72314957-72314976 MS.gene77500:CDS 50.0%
TCTGAGGAAAAGAGTGGTGG+TGG - chr3.2:72315842-72315861 MS.gene77500:CDS 50.0%
TGGAAGCTGTACAGCAAGAG+TGG + chr3.2:72315108-72315127 None:intergenic 50.0%
TGTTGGTCGGCCAAAAACGA+CGG - chr3.2:72315521-72315540 MS.gene77500:intron 50.0%
TTGGCACTACCACCAACGTA+TGG + chr3.2:72314969-72314988 None:intergenic 50.0%
!! GGAAAAGAGTGGTGGTGGAT+TGG - chr3.2:72315847-72315866 MS.gene77500:CDS 50.0%
AGTTGAATTAGGCACGGGGC+CGG + chr3.2:72315569-72315588 None:intergenic 55.0%
!! AGAGTGGTGGTGGATTGGGA+AGG - chr3.2:72315852-72315871 MS.gene77500:CDS 55.0%
ATTGGGAAGGAGGTGGCTGG+TGG - chr3.2:72315865-72315884 MS.gene77500:CDS 60.0%
! TGGATTGGGAAGGAGGTGGC+TGG - chr3.2:72315862-72315881 MS.gene77500:CDS 60.0%
!! GTGGTGGATTGGGAAGGAGG+TGG - chr3.2:72315858-72315877 MS.gene77500:CDS 60.0%
!! GTGGTGGTGGATTGGGAAGG+AGG - chr3.2:72315855-72315874 MS.gene77500:CDS 60.0%
AAGGAGGTGGCTGGTGGCAG+AGG - chr3.2:72315871-72315890 MS.gene77500:CDS 65.0%
GAGGTGGCTGGTGGCAGAGG+TGG - chr3.2:72315874-72315893 MS.gene77500:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 72314840 72315949 72314840 ID=MS.gene77500
chr3.2 mRNA 72314840 72315949 72314840 ID=MS.gene77500.t1;Parent=MS.gene77500
chr3.2 exon 72315628 72315949 72315628 ID=MS.gene77500.t1.exon1;Parent=MS.gene77500.t1
chr3.2 CDS 72315628 72315949 72315628 ID=cds.MS.gene77500.t1;Parent=MS.gene77500.t1
chr3.2 exon 72315065 72315160 72315065 ID=MS.gene77500.t1.exon2;Parent=MS.gene77500.t1
chr3.2 CDS 72315065 72315160 72315065 ID=cds.MS.gene77500.t1;Parent=MS.gene77500.t1
chr3.2 exon 72314840 72314976 72314840 ID=MS.gene77500.t1.exon3;Parent=MS.gene77500.t1
chr3.2 CDS 72314840 72314976 72314840 ID=cds.MS.gene77500.t1;Parent=MS.gene77500.t1
Gene Sequence

>MS.gene77500

ATGATGGTTTCCAACAAAAGTGTAACTGCATTATTATTTCTACTGTTGGCTGGTTTTGTGAGCTCAGATTTTACAAAAGACAGAAACGATTGTGCTGATAAACTTGTTACTCTTGCTGGTTGTCTTCCATACGTTGGTGGTAGTGCCAATACCCCCACTATAGATTGTTGTACTTCCCTTAAACAAGTCCTTGATAAGACCAAAAAATGCATCTGTATCCTCATCAAAGATCGCAATGACCCCAAACTTGGTATCAAGTTGAATGCCACTCTTGCTGTACAGCTTCCAAACGCTTGTCACATACCATCTAATATAACTGAATGTGTAGGCAAGTATATTTTTCTCATGATCTTATCTAGCTATTAGTTTAGTTTGCTTTATGTATTAAGGGCCTGATTGGACTATATACATATGCTGTTTAAGCTGATTTTCATAAACTTTTCAAATAGCTTATGAAAACAACTTATAGATTATACAGAAATAATTTGACTTTATTATACATAAATGATATACGTTATAAATGACATAAGTGTTTATTAAAATGTTTATATAAATAAGGCTTAATCATGGCCGGTCCAATAATCTTCAAAAGTAAGTGAGGCATAAAGCCAAGTTCAATATATGTCTCCTCTTTTGTCACATGAGACATTTTTCTTGTAAAATCTAATGAGAATTTTTTTTTGTTGGTCGGCCAAAAACGACGGCTTTAGTAGCTTTACTTTTGCGCCGGCCCCGTGCCTAATTCAACTCAATGGAATGTTTAATTTATACTTTTGTATTTTTTTCCAGATCTTCTACATTTGTCACCTAAATCCCCTGAAGCTAAGGTGTTTGAAGGACTTGGAAATTCTACCAAAACAAACAGTTCCTCCCCTATTTCCAGCGGTAATTATTATAATTTTAGTATTGTTATTGAGTAATTTTTGCGTGTATTGAAAATTGAAGCTAATTTGTGCCTCTAATAATGTTTAAGGTTCTGCTGAGAGGGGATCAAGCTCAAGTTCTGAGGAAAAGAGTGGTGGTGGATTGGGAAGGAGGTGGCTGGTGGCAGAGGTGGTTTGTGCTATTTTACCATTTCTTTTCATATCTCACTTCTTCATCCTAGCTTGA

Protein sequence

>MS.gene77500.t1

MMVSNKSVTALLFLLLAGFVSSDFTKDRNDCADKLVTLAGCLPYVGGSANTPTIDCCTSLKQVLDKTKKCICILIKDRNDPKLGIKLNATLAVQLPNACHIPSNITEYLLHLSPKSPEAKVFEGLGNSTKTNSSSPISSGSAERGSSSSSEEKSGGGLGRRWLVAEVVCAILPFLFISHFFILA