AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene018645

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene018645.t1 MTR_7g098430 98.184 826 15 0 1 826 1 826 0.0 1674
MS.gene018645.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 2.07e-134 429
MS.gene018645.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 2.10e-134 429
MS.gene018645.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 2.11e-134 429
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene018645.t1 AT1G04080 65.057 704 224 9 109 795 37 735 0.0 915
MS.gene018645.t1 AT1G04080 65.057 704 224 9 109 795 37 735 0.0 915
MS.gene018645.t1 AT1G04080 59.527 761 227 12 109 795 37 790 0.0 867
MS.gene018645.t1 AT1G04080 65.027 549 173 7 261 795 6 549 0.0 704
MS.gene018645.t1 AT1G04080 63.916 521 169 7 289 795 1 516 0.0 652
MS.gene018645.t1 AT5G46400 41.392 546 296 9 154 687 30 563 2.93e-128 410
MS.gene018645.t1 AT5G46400 40.000 325 188 4 364 687 25 343 2.42e-66 237
MS.gene018645.t1 AT5G46400 40.000 325 188 4 364 687 25 343 2.42e-66 237
MS.gene018645.t1 AT4G24270 25.392 319 185 14 162 465 59 339 8.68e-11 66.2
MS.gene018645.t1 AT4G24270 25.392 319 185 14 162 465 59 339 8.68e-11 66.2

Find 211 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTATGCATTTACTTATTC+TGG 0.134991 7.1:-10242338 MS.gene018645:CDS
GCTTCTCCTGCAGCCTCGGC+TGG 0.142915 7.1:+10242383 None:intergenic
TTATTCTGTCATTAGGCTTC+TGG 0.145350 7.1:-10237002 MS.gene018645:intron
TATCGATCCAATTGCTGATT+TGG 0.218895 7.1:+10239729 None:intergenic
CTTGCTACACCAGGGTTCTC+TGG 0.220827 7.1:+10236664 None:intergenic
ATTTGGATTCTCTAATATTC+TGG 0.228707 7.1:+10239746 None:intergenic
TGAGCTTGTAGTACAGAATT+AGG 0.232263 7.1:+10242287 None:intergenic
CAGAATTCCCTTTGTGTTAT+GGG 0.232733 7.1:-10240661 MS.gene018645:CDS
ATTATCTTTCGTTTCCTCTT+TGG 0.236191 7.1:-10239830 MS.gene018645:CDS
ACGTACGACCTCTTAATATT+GGG 0.271534 7.1:-10239249 MS.gene018645:CDS
TGAAAACTGGCATAATTATC+TGG 0.271539 7.1:-10239221 MS.gene018645:CDS
CTTATACATCTCTTCTCTAA+TGG 0.271968 7.1:+10239336 None:intergenic
CCAAACTGTTCTTGCCAAAC+AGG 0.276139 7.1:-10236447 MS.gene018645:CDS
GATACAGTTGGACTTGAAAA+TGG 0.279480 7.1:-10242137 MS.gene018645:CDS
CACGTACGACCTCTTAATAT+TGG 0.285155 7.1:-10239250 MS.gene018645:CDS
CTTCTCCTGCAGCCTCGGCT+GGG 0.286804 7.1:+10242384 None:intergenic
TTATCTTTCGTTTCCTCTTT+GGG 0.287270 7.1:-10239829 MS.gene018645:CDS
GCTGCTGCTCTGCCCAATTC+AGG 0.288615 7.1:-10242359 MS.gene018645:CDS
GCAGAATTCCCTTTGTGTTA+TGG 0.289100 7.1:-10240662 MS.gene018645:CDS
GCTGACCATTCATCTGTAAA+TGG 0.289348 7.1:-10242167 MS.gene018645:CDS
CGGCTGCAGGTTGCTGTGTC+GGG 0.291007 7.1:+10235079 None:intergenic
AATACAGCACTTGCAGGTTT+AGG 0.295664 7.1:+10239391 None:intergenic
CCTTGTGCTTGAGTCGTTGC+TGG 0.297776 7.1:+10236259 None:intergenic
AGTAAATGCATAGCCTGAAT+TGG 0.309418 7.1:+10242346 None:intergenic
AATAAACAAATCGAGAATAC+TGG 0.315523 7.1:+10237448 None:intergenic
TGCTGTGTCGGGGCTGCAAC+AGG 0.320209 7.1:+10235090 None:intergenic
GTTCCACAAAGCACTGCTTA+TGG 0.321030 7.1:-10235626 MS.gene018645:CDS
TCCCTTTGTGTTATGGGTAT+TGG 0.321785 7.1:-10240655 MS.gene018645:CDS
TATTCTGTCATTAGGCTTCT+GGG 0.323007 7.1:-10237001 MS.gene018645:intron
TATGGGGCTTATGCTGGATA+TGG 0.331066 7.1:-10235683 MS.gene018645:CDS
GTATTCTCGATTTGTTTATT+TGG 0.332612 7.1:-10237445 MS.gene018645:intron
TATCCAGTACTCGGGATAAT+TGG 0.333216 7.1:+10238635 None:intergenic
GCCGTTCCTCAGGCATATTA+TGG 0.342067 7.1:-10235060 MS.gene018645:CDS
TGCCTTCTCTTTGTATGAAC+AGG 0.345610 7.1:-10237526 MS.gene018645:CDS
ATCGACCTCTTTCAGAATTA+CGG 0.355782 7.1:-10239510 MS.gene018645:CDS
CCTAACCAATGGCCTAACTA+TGG 0.364535 7.1:-10236301 MS.gene018645:CDS
GGCCATTGGTTAGGTCCATT+TGG 0.367818 7.1:+10236310 None:intergenic
TTCCACAAAGCACTGCTTAT+GGG 0.376637 7.1:-10235625 MS.gene018645:CDS
CCTGTTTGGCAAGAACAGTT+TGG 0.377400 7.1:+10236447 None:intergenic
TACACCAGGGTTCTCTGGGT+TGG 0.381425 7.1:+10236669 None:intergenic
TATGGAAGCTAGGGAAAGTA+TGG 0.382672 7.1:-10238598 MS.gene018645:CDS
TGCTGCTGGGTGTAGCCAGC+AGG 0.383369 7.1:+10236226 None:intergenic
TCTGTTAATACAGCACTTGC+AGG 0.384276 7.1:+10239385 None:intergenic
ATGAGTACATGCAGCAAGAC+TGG 0.385484 7.1:-10239791 MS.gene018645:CDS
CAATGTCAACTCGCTTTGGC+TGG 0.386893 7.1:+10236728 None:intergenic
AATAATCAGGCATGCCAATA+TGG 0.389365 7.1:-10237657 MS.gene018645:CDS
CCTGCAGCCTCGGCTGGGGC+AGG 0.390779 7.1:+10242389 None:intergenic
GAGATGTTCAATCGATCAAA+AGG 0.399956 7.1:-10236486 MS.gene018645:CDS
AGCAGCTTCTCCTGCAGCCT+CGG 0.403142 7.1:+10242379 None:intergenic
GATGAAGCTGCTGCAGTGGC+AGG 0.405134 7.1:-10239481 MS.gene018645:CDS
ACACCAGGGTTCTCTGGGTT+GGG 0.411863 7.1:+10236670 None:intergenic
TGAGGCTGTACTCCATAGTT+AGG 0.412997 7.1:+10236289 None:intergenic
ATACAGGCTGCCGCTTCCTA+TGG 0.418623 7.1:-10235701 MS.gene018645:intron
AACTCCGACAAGCCCCTGTT+TGG 0.419402 7.1:+10236433 None:intergenic
TTCTCCTGCAGCCTCGGCTG+GGG 0.420645 7.1:+10242385 None:intergenic
TTGCGCTCGGTTCAAGGAGC+AGG 0.422648 7.1:-10237753 MS.gene018645:CDS
GGAGAATTATCTGCACCATC+AGG 0.426487 7.1:+10239412 None:intergenic
AATGTCAACTCGCTTTGGCT+GGG 0.427195 7.1:+10236729 None:intergenic
TATTACCTACGAACTGTGTC+TGG 0.428305 7.1:+10240491 None:intergenic
CATGATATTGCAGGGGAAGT+TGG 0.428308 7.1:-10237553 MS.gene018645:intron
ACGGCTGCAGGTTGCTGTGT+CGG 0.430705 7.1:+10235078 None:intergenic
TTCACCTGTGCAGGATATGC+AGG 0.431301 7.1:+10235593 None:intergenic
TTAGCACCAACCTTGGGAAC+AGG 0.431376 7.1:+10242416 None:intergenic
TAAATGGTGTTGCAGCTTCA+AGG 0.434451 7.1:-10239545 MS.gene018645:intron
TACAGGCTGCCGCTTCCTAT+GGG 0.435003 7.1:-10235700 MS.gene018645:intron
CCTGCCCCAGCCGAGGCTGC+AGG 0.435556 7.1:-10242389 MS.gene018645:CDS
GCATTTACTTATTCTGGTGA+TGG 0.435692 7.1:-10242332 MS.gene018645:CDS
TACCAATCGATGTTCCATAT+TGG 0.436137 7.1:+10237643 None:intergenic
GATGGGAATGCTTATACTGC+TGG 0.441620 7.1:-10242314 MS.gene018645:CDS
CCGTTCCTCAGGCATATTAT+GGG 0.443253 7.1:-10235059 MS.gene018645:CDS
AATTGGATCGATATTTCAAC+AGG 0.444059 7.1:-10239719 MS.gene018645:intron
AAAACCAATTTACCTGCTGC+TGG 0.444538 7.1:+10236212 None:intergenic
GTCTTCTTGAAGCAATAATC+AGG 0.448175 7.1:-10237670 MS.gene018645:CDS
AGAGAATCCAAATCAGCAAT+TGG 0.448924 7.1:-10239736 MS.gene018645:CDS
TTGCTACACCAGGGTTCTCT+GGG 0.452718 7.1:+10236665 None:intergenic
GTTCACACTGAAATTTCTCC+AGG 0.453659 7.1:-10237692 MS.gene018645:CDS
CATTGACTTTCTAGAGTCAC+TGG 0.456681 7.1:-10236710 MS.gene018645:CDS
GATATGCACCCACTACAGAT+TGG 0.457141 7.1:+10236332 None:intergenic
GCCCCATAAGCAGTGCTTTG+TGG 0.457630 7.1:+10235623 None:intergenic
ATATTGGGGAGCTTGAAAAC+TGG 0.459736 7.1:-10239234 MS.gene018645:CDS
GCTGACCATGAGGCGCGCCT+TGG 0.460933 7.1:-10240623 MS.gene018645:CDS
GCTTCCTATGGGGCTTATGC+TGG 0.462340 7.1:-10235689 MS.gene018645:CDS
TCTGGTTGGAAAGGAAGCTC+TGG 0.463748 7.1:-10242207 MS.gene018645:CDS
CTTGCCAAACAGGGGCTTGT+CGG 0.464209 7.1:-10236437 MS.gene018645:CDS
GAGTTCGATTCTAAGATCAT+TGG 0.466817 7.1:-10239304 MS.gene018645:CDS
CATTTACTTATTCTGGTGAT+GGG 0.468008 7.1:-10242331 MS.gene018645:CDS
CACTTCCATTTACAGATGAA+TGG 0.468952 7.1:+10242162 None:intergenic
CCATAGTTAGGCCATTGGTT+AGG 0.469295 7.1:+10236301 None:intergenic
TGCTGGCTACACCCAGCAGC+AGG 0.471209 7.1:-10236224 MS.gene018645:intron
TCGAATCCTGTTCCCAAGGT+TGG 0.472109 7.1:-10242422 MS.gene018645:CDS
AACGAGTCAAAGCAAGCTGC+TGG 0.475326 7.1:-10242251 MS.gene018645:CDS
TCAAGCTCCCCAATATTAAG+AGG 0.477060 7.1:+10239241 None:intergenic
GTTTATGAACGAGCTGTTCA+AGG 0.479117 7.1:-10240578 MS.gene018645:CDS
GAACGAGGATTAGCTTATGT+TGG 0.479715 7.1:-10239858 MS.gene018645:CDS
AGCTTCCTTTCCAACCAGAC+TGG 0.482155 7.1:+10242211 None:intergenic
TGCAAATAATGTTCTCGCTC+GGG 0.483808 7.1:-10238571 MS.gene018645:CDS
GTAAATGCATAGCCTGAATT+GGG 0.486099 7.1:+10242347 None:intergenic
ATGTCAACTCGCTTTGGCTG+GGG 0.492568 7.1:+10236730 None:intergenic
GTGCCAATTATCCCGAGTAC+TGG 0.494096 7.1:-10238638 MS.gene018645:CDS
AAGTCAATGTCAACTCGCTT+TGG 0.496185 7.1:+10236724 None:intergenic
CAAGGACAACAATGGCCTGC+TGG 0.496647 7.1:-10236241 MS.gene018645:CDS
GCCTAATGAGGATTCCAGTC+TGG 0.497327 7.1:-10242225 MS.gene018645:CDS
ATTAACAGAAGCAGAAGAGT+TGG 0.497386 7.1:-10239371 MS.gene018645:CDS
GGAACTGATGCAGGCAATTG+TGG 0.498199 7.1:+10235644 None:intergenic
TCCAATACCCATAACACAAA+GGG 0.499353 7.1:+10240654 None:intergenic
CAGGCCATTGCCATTGAGAA+AGG 0.499761 7.1:-10237507 MS.gene018645:CDS
TATGCCTGAGGAACGGCTGC+AGG 0.506298 7.1:+10235066 None:intergenic
GCAACCTGCAGCCGTTCCTC+AGG 0.509331 7.1:-10235070 MS.gene018645:CDS
AAGAGGTCGTACGTGAAAGT+AGG 0.516439 7.1:+10239258 None:intergenic
GGCTTCTGGGAATTCCGAAA+AGG 0.517187 7.1:-10236988 MS.gene018645:CDS
GGTACTGACCAAGGTGTTGA+AGG 0.517289 7.1:-10239445 MS.gene018645:CDS
GAGCAGGCTGGAGATATTGT+TGG 0.520258 7.1:-10237737 MS.gene018645:CDS
CAGAGCCAAGGCGCGCCTCA+TGG 0.522186 7.1:+10240618 None:intergenic
ATATGCACCCACTACAGATT+GGG 0.523107 7.1:+10236333 None:intergenic
CGAGACAGAGAAGGTGGCAG+AGG 0.524685 7.1:-10241105 MS.gene018645:intron
GGAACATTAGCACCAACCTT+GGG 0.526018 7.1:+10242410 None:intergenic
TGCCAATATGGAACATCGAT+TGG 0.531852 7.1:-10237645 MS.gene018645:intron
TTGCAAATAATGTTCTCGCT+CGG 0.532036 7.1:-10238572 MS.gene018645:CDS
TCCTCCTGCATATCCTGCAC+AGG 0.532285 7.1:-10235597 MS.gene018645:intron
CGCTTCGAATCCTGTTCCCA+AGG 0.532298 7.1:-10242426 MS.gene018645:CDS
GTGTTGCAGCTTCAAGGAGT+TGG 0.532516 7.1:-10239539 MS.gene018645:intron
GTCGTTGCTGGCCAAGTTTG+AGG 0.533522 7.1:+10236271 None:intergenic
ATGGAGTACAGCCTCAAACT+TGG 0.534256 7.1:-10236282 MS.gene018645:CDS
ATGGGAATGCTTATACTGCT+GGG 0.536210 7.1:-10242313 MS.gene018645:CDS
ACCAGACTGGAATCCTCATT+AGG 0.536266 7.1:+10242224 None:intergenic
AGGAACATTAGCACCAACCT+TGG 0.537288 7.1:+10242409 None:intergenic
AACATAGCGTATCCAGTACT+CGG 0.538429 7.1:+10238626 None:intergenic
TCTGTTGCACTTGCTACACC+AGG 0.538737 7.1:+10236655 None:intergenic
TCAATGTATTTATCCCAAAG+AGG 0.538798 7.1:+10239816 None:intergenic
ATATTCGCTATAAGCACCTA+TGG 0.541316 7.1:-10240518 MS.gene018645:CDS
GCTCGGTTCAAGGAGCAGGC+TGG 0.541555 7.1:-10237749 MS.gene018645:CDS
CAAACTGTTCTTGCCAAACA+GGG 0.544913 7.1:-10236446 MS.gene018645:CDS
AGCATAAGCCCCATAGGAAG+CGG 0.546523 7.1:+10235691 None:intergenic
GCTGTCCGTAATTCTGAAAG+AGG 0.548224 7.1:+10239505 None:intergenic
GGCTGCAGGTTGCTGTGTCG+GGG 0.548629 7.1:+10235080 None:intergenic
GTGGCAGGTGTTGTTTCAGA+GGG 0.550122 7.1:-10239466 MS.gene018645:CDS
GTACTCCATAGTTAGGCCAT+TGG 0.550599 7.1:+10236296 None:intergenic
AATGCATCAGAGAATGTTGA+TGG 0.550820 7.1:-10242113 MS.gene018645:CDS
TCCACAAAGCACTGCTTATG+GGG 0.553188 7.1:-10235624 MS.gene018645:CDS
AGTGGCAGGTGTTGTTTCAG+AGG 0.553746 7.1:-10239467 MS.gene018645:CDS
GTGCTTTGTGGAACTGATGC+AGG 0.555027 7.1:+10235635 None:intergenic
AAACAACTCACAGATGCTTA+CGG 0.557128 7.1:-10242077 MS.gene018645:intron
AGCTGATGAAGCTGCTGCAG+TGG 0.558284 7.1:-10239485 MS.gene018645:CDS
GGTGTAAATCATTGCAAGGC+GGG 0.558474 7.1:+10239767 None:intergenic
GGGGCTTATGCTGGATATGG+AGG 0.560147 7.1:-10235680 MS.gene018645:CDS
AACTGTGTCTGGATCTCCAT+AGG 0.560352 7.1:+10240502 None:intergenic
TTTATGAACGAGCTGTTCAA+GGG 0.561629 7.1:-10240577 MS.gene018645:CDS
ATTATTGCTTCAAGAAGACC+TGG 0.562102 7.1:+10237674 None:intergenic
AATGAGGATTCCAGTCTGGT+TGG 0.562814 7.1:-10242221 MS.gene018645:CDS
AGAGGTCGTACGTGAAAGTA+GGG 0.564513 7.1:+10239259 None:intergenic
GGAAGCTCTGGACTCTGCTA+TGG 0.564721 7.1:-10242195 MS.gene018645:CDS
ATCACCTGCCCAATCTGTAG+TGG 0.565221 7.1:-10236341 MS.gene018645:CDS
AGATGTTCAATCGATCAAAA+GGG 0.566435 7.1:-10236485 MS.gene018645:CDS
GTAGTGGGTGCATATCCAAA+TGG 0.568266 7.1:-10236325 MS.gene018645:CDS
GGATTCCAGTCTGGTTGGAA+AGG 0.569228 7.1:-10242216 MS.gene018645:CDS
TTTGATTGACGAGACAGAGA+AGG 0.570064 7.1:-10241114 MS.gene018645:CDS
AAACCAATTTACCTGCTGCT+GGG 0.571184 7.1:+10236213 None:intergenic
TCAATCGATCAAAAGGGTTG+AGG 0.572118 7.1:-10236479 MS.gene018645:CDS
GATATTGCAGGGGAAGTTGG+AGG 0.576699 7.1:-10237550 MS.gene018645:intron
TAATGTTCCTGCCCCAGCCG+AGG 0.579939 7.1:-10242396 MS.gene018645:CDS
AAGAGAAGGCGACTTGAGCA+AGG 0.580408 7.1:-10239188 MS.gene018645:intron
TGCAGGATATGCAGGAGGAT+AGG 0.581110 7.1:+10235601 None:intergenic
TTCTGGTGTAAATCATTGCA+AGG 0.581222 7.1:+10239763 None:intergenic
GAACTGATGCAGGCAATTGT+GGG 0.582647 7.1:+10235645 None:intergenic
AGCTGCTGGAGTGCCTAATG+AGG 0.586064 7.1:-10242237 MS.gene018645:CDS
TGCTGCTGAGACGTATCCAT+CGG 0.586175 7.1:-10242465 MS.gene018645:CDS
AATGGAAGTGTTGATACAGT+TGG 0.591174 7.1:-10242149 MS.gene018645:CDS
CCCATAATATGCCTGAGGAA+CGG 0.592953 7.1:+10235059 None:intergenic
ATAAACAAATCGAGAATACT+GGG 0.595828 7.1:+10237449 None:intergenic
TGAGTACATGCAGCAAGACT+GGG 0.596076 7.1:-10239790 MS.gene018645:CDS
CAACTTTATCAGCAGAGCCA+AGG 0.596207 7.1:+10240606 None:intergenic
CGTACGACCTCTTAATATTG+GGG 0.599762 7.1:-10239248 MS.gene018645:CDS
TTACATATTCAGTGGACATG+TGG 0.600677 7.1:-10240553 MS.gene018645:CDS
ATATCCAGCATAAGCCCCAT+AGG 0.601936 7.1:+10235685 None:intergenic
ACTGTCAAAGCCACTACTTG+AGG 0.604117 7.1:-10236928 MS.gene018645:intron
AGCAAGTGCAACAGAGCGCG+AGG 0.605392 7.1:-10236647 MS.gene018645:CDS
GAATACTGGGCAAACAACAT+TGG 0.606890 7.1:+10237462 None:intergenic
TCAAGGGGTTACATATTCAG+TGG 0.607137 7.1:-10240561 MS.gene018645:CDS
GCACCCACTACAGATTGGGC+AGG 0.607563 7.1:+10236337 None:intergenic
TCACCTGCCCAATCTGTAGT+GGG 0.612384 7.1:-10236340 MS.gene018645:CDS
TTCCAATACCCATAACACAA+AGG 0.612695 7.1:+10240653 None:intergenic
AACACAAAGGGAATTCTGCT+AGG 0.613157 7.1:+10240666 None:intergenic
TATGGGAGATAGTGAAACAA+TGG 0.615550 7.1:-10242501 MS.gene018645:CDS
TGACCAAGGTGTTGAAGGAG+AGG 0.619456 7.1:-10239440 MS.gene018645:CDS
GTGTTGTTGTAAGCATCCGA+TGG 0.622239 7.1:+10242449 None:intergenic
ACATAGCGTATCCAGTACTC+GGG 0.622860 7.1:+10238627 None:intergenic
ATCCAAATGGACCTAACCAA+TGG 0.623577 7.1:-10236312 MS.gene018645:CDS
ATCAGAGAATGTTGATGGGT+CGG 0.627186 7.1:-10242108 MS.gene018645:CDS
TTGAGACAAACCTCAAGTAG+TGG 0.627545 7.1:+10236918 None:intergenic
CTGCTGAAGAAGATCGATTG+TGG 0.627715 7.1:-10241181 MS.gene018645:CDS
GGCCTGTTCATACAAAGAGA+AGG 0.629259 7.1:+10237524 None:intergenic
GATTGACGAGACAGAGAAGG+TGG 0.630394 7.1:-10241111 MS.gene018645:CDS
ACAGGCTGCCGCTTCCTATG+GGG 0.637522 7.1:-10235699 MS.gene018645:intron
GTACTCGGGATAATTGGCAC+AGG 0.638207 7.1:+10238641 None:intergenic
CTCAAGCACAAGGACAACAA+TGG 0.638781 7.1:-10236249 MS.gene018645:CDS
GTTTCAGAGGGTACTGACCA+AGG 0.640522 7.1:-10239454 MS.gene018645:CDS
GAAGGAGAGGTTCATCCTGA+TGG 0.646457 7.1:-10239427 MS.gene018645:CDS
GAACCTCTCCTTCAACACCT+TGG 0.647061 7.1:+10239437 None:intergenic
ATGCATCAGAGAATGTTGAT+GGG 0.647426 7.1:-10242112 MS.gene018645:CDS
CTGTTGCACTTGCTACACCA+GGG 0.651331 7.1:+10236656 None:intergenic
ACCTGTGCAGGATATGCAGG+AGG 0.651957 7.1:+10235596 None:intergenic
AAACTGTTCTTGCCAAACAG+GGG 0.652207 7.1:-10236445 MS.gene018645:CDS
ACACCCAACCCAGAGAACCC+TGG 0.662263 7.1:-10236673 MS.gene018645:CDS
TTATGAACGAGCTGTTCAAG+GGG 0.665698 7.1:-10240576 MS.gene018645:CDS
CCAGCAACGACTCAAGCACA+AGG 0.666384 7.1:-10236259 MS.gene018645:CDS
GAGATCCAGACACAGTTCGT+AGG 0.679080 7.1:-10240496 MS.gene018645:intron
TGGGAATGCTTATACTGCTG+GGG 0.686552 7.1:-10242312 MS.gene018645:CDS
GAAGAAATATGCTGACCATG+AGG 0.691106 7.1:-10240633 MS.gene018645:CDS
TAATTCCCATAATATGCCTG+AGG 0.692350 7.1:+10235054 None:intergenic
TGGTGTAAATCATTGCAAGG+CGG 0.722048 7.1:+10239766 None:intergenic
CTTGGGAACAGGATTCGAAG+CGG 0.730245 7.1:+10242427 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr7.1 gene 10235047 10242522 10235047 ID=MS.gene018645
chr7.1 mRNA 10235047 10242522 10235047 ID=MS.gene018645.t1;Parent=MS.gene018645
chr7.1 exon 10242078 10242522 10242078 ID=MS.gene018645.t1.exon1;Parent=MS.gene018645.t1
chr7.1 CDS 10242078 10242522 10242078 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10241106 10241212 10241106 ID=MS.gene018645.t1.exon2;Parent=MS.gene018645.t1
chr7.1 CDS 10241106 10241212 10241106 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10240497 10240726 10240497 ID=MS.gene018645.t1.exon3;Parent=MS.gene018645.t1
chr7.1 CDS 10240497 10240726 10240497 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10239720 10239887 10239720 ID=MS.gene018645.t1.exon4;Parent=MS.gene018645.t1
chr7.1 CDS 10239720 10239887 10239720 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10239189 10239552 10239189 ID=MS.gene018645.t1.exon5;Parent=MS.gene018645.t1
chr7.1 CDS 10239189 10239552 10239189 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10238551 10238694 10238551 ID=MS.gene018645.t1.exon6;Parent=MS.gene018645.t1
chr7.1 CDS 10238551 10238694 10238551 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10237646 10237798 10237646 ID=MS.gene018645.t1.exon7;Parent=MS.gene018645.t1
chr7.1 CDS 10237646 10237798 10237646 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10237446 10237562 10237446 ID=MS.gene018645.t1.exon8;Parent=MS.gene018645.t1
chr7.1 CDS 10237446 10237562 10237446 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10236929 10237009 10236929 ID=MS.gene018645.t1.exon9;Parent=MS.gene018645.t1
chr7.1 CDS 10236929 10237009 10236929 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10236624 10236779 10236624 ID=MS.gene018645.t1.exon10;Parent=MS.gene018645.t1
chr7.1 CDS 10236624 10236779 10236624 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10236225 10236524 10236225 ID=MS.gene018645.t1.exon11;Parent=MS.gene018645.t1
chr7.1 CDS 10236225 10236524 10236225 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10235598 10235717 10235598 ID=MS.gene018645.t1.exon12;Parent=MS.gene018645.t1
chr7.1 CDS 10235598 10235717 10235598 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
chr7.1 exon 10235047 10235142 10235047 ID=MS.gene018645.t1.exon13;Parent=MS.gene018645.t1
chr7.1 CDS 10235047 10235142 10235047 ID=cds.MS.gene018645.t1;Parent=MS.gene018645.t1
Gene Sequence

>MS.gene018645

ATGGGAGATAGTGAAACAATGGTTGCAGAAGCATCTGCTGCTGAGACGTATCCATCGGATGCTTACAACAACACCGCTTCGAATCCTGTTCCCAAGGTTGGTGCTAATGTTCCTGCCCCAGCCGAGGCTGCAGGAGAAGCTGCTGCTGCTCTGCCCAATTCAGGCTATGCATTTACTTATTCTGGTGATGGGAATGCTTATACTGCTGGGGATCCTAATTCTGTACTACAAGCTCAATTTAGTGCAACAAACGAGTCAAAGCAAGCTGCTGGAGTGCCTAATGAGGATTCCAGTCTGGTTGGAAAGGAAGCTCTGGACTCTGCTATGGTCTCTGCTGACCATTCATCTGTAAATGGAAGTGTTGATACAGTTGGACTTGAAAATGGAAATGCATCAGAGAATGTTGATGGGTCGGCTGATGAGAAACAACTCACAGATGCTTACGGTATGTATATGCCTTCTCTGTCTGTTATGTTAGCATACTCTTCGGGAAAAGTGAAGTTATTTCGTTTTTATTGTTATTGAAGTTTTGTTAATAAAAAAATCGGAGTTCAATGTTAAGGGTATGTATAGAGCTTGCAAGTAATATAATTAACCAATGATGGAGTCAAGGACTTAAGATTCAAGTGATTTATTATTAACCAGCAAATAGTTCATTTAGGCCTTGTAGTTCATGTAAGCTTGTAAGTAATACAATTGCATATCAATGTTGAGAACTAATGAATTTTCACCAATATTCATGAATGGAAATAAGAAAATTTTAATCGCTCATAGTAACAATAGTGTTGTCAATTGTGGATAACGGATAAAACAGTAGCGGTTTGTTAATATTCCGCTACGCTATAGCGGTATAGTGGTTGCTGTTTAGCAACACTGAGTAAGAAGCTTTATTTTTTCATGCATTGGAGGTGGCGTTTAAGGCTACTTCCATTTGTTTTTGTCTCAAGTGACTTTGAAATTTTTCCAGGCCTGGTTTGAATTGGATCTTGACCATATTGATATAATAACTAGGTTTATTAGGTGTATAAATACGTTCATACATTTACATTTTTTTTCCATTTTATTGGTTTACCAGTGCAACAAGATATCATGACTTTGGTTTGTAATTTGATGATCACATATTCTAAGCCACTTCTGCGATTTTTGGATGTCTTCTATTTTACTGAGTTGGCTGATTCAGGCCTAAACATACACGTCTTATCTCCATACGTCTTGTTTTTAATATTATCTTGTTATTAATTATTCACAAGCATATTGCTGTTCAACCAAATTATGGTTTTGCTGCTAATCATTTATCTCACGTTTAACAGCTGCGTTGTCTGCTGAAGAAGATCGATTGTGGAATATTGTAACAGCTAATTCTTCTGATTTTACTGCTTGGACTTCTTTGATTGACGAGACAGAGAAGGTGGCAGAGGTATGATATGTTGACCCAATTTTTAAGACGTGAATTACATGTCGGGGAATCAAAATAAATGACTGATCTCGATGTTTTCATATTAGTTTGATATATGACATGCATGCATTATGATTCAGTATACTACGTGTTTAATGAGATGGATAATTTATATTGTTAAATACTTAAACCTGCATCTGTTTAACGGGGGTGTCTTGATTAAGAATGTCTATAATGTACACACAAGTTATGTGGGTGATTTCTCTGGTTCTGTCTGTCGGTATATTATTTTTTAACCTGCTAGTATATTATTTAGTGGCAAACTAATGTGATGTATTTGATTTTATTATTGATTTATTTTATGACTCATGGTTCTCATATAATGATTTTTTTATTGAAGAACAATATTTTGAAGATGCGTAGAGTCTATGATGCTTTCCTAGCAGAATTCCCTTTGTGTTATGGGTATTGGAAGAAATATGCTGACCATGAGGCGCGCCTTGGCTCTGCTGATAAAGTTGTTGAAGTTTATGAACGAGCTGTTCAAGGGGTTACATATTCAGTGGACATGTGGTTGCATTATTGCATATTCGCTATAAGCACCTATGGAGATCCAGACACAGTTCGTAGGTAATAGAGGCTTATTTACATGTTACCTTATTATTTTCTATGATCGGATTTTCTATTTACTAGGCTCAGCTGTTTCTTGCTTGCTTTCTAACCTGATTTCAGAGACCGTGCAACAGTACCATCTTTTTATGGCGGAAAAGCTTTGATGGGATTGCATGTCAGAATGTTTTCCATTCTGATGTTTAAATCCTTGGTTTTGATTCCACTATGTATAGTAGTTGAAATTTTGGGTCCATTTTCCCCTATAAACTTTTCAATTTGATTTCAAACTTCATAGTTGTGTGGAAGGGTTCTATTTTCCCCCCCTTTCCTGTTGAAATGGTTAGCTGTTTTTATCTTTGACACTATGAGGTGTATCAATTTGCAAATTGCTGCTGGGAAGATCTCGTCTGGTTAAGGGCTAGATGGCCTCTATGACTGCTCCATTTCTCGGACATTGTATGATTTTTTGCAGGAAGGTAAATGCAGCGTTTGGATGTCTTATATGATGTTACGAGTGATTTGTTTGTTACCTTAGAACACTAGTGCTGCTGTTTCCCATACATGGATGTGCATCAGTAAATGGATGCTTTAAGTTACAGATTAATCAAGACTGCTTGCTTATTTCAGGCTTTTTGAACGAGGATTAGCTTATGTTGGAACAGATTATCTTTCGTTTCCTCTTTGGGATAAATACATTGAATATGAGTACATGCAGCAAGACTGGGCCCGCCTTGCAATGATTTACACCAGAATATTAGAGAATCCAAATCAGCAATTGGATCGATATTTCAACAGGTAATTACTTGTTTTAATTTAATATTATCATTCCACTTCATTTGTTTCCAAAAAAGAAACACAATTACTATTTTTTGTTTTATATTTCTACATAATATCCCCTTCTACTATCTCCTCTCTATTTTCCCCCGAACTAAATTACTTCAACGCTGTAAATGGTGTTGCAGCTTCAAGGAGTTGGCTAGTAATCGACCTCTTTCAGAATTACGGACAGCTGATGAAGCTGCTGCAGTGGCAGGTGTTGTTTCAGAGGGTACTGACCAAGGTGTTGAAGGAGAGGTTCATCCTGATGGTGCAGATAATTCTCCTAAACCTGCAAGTGCTGTATTAACAGAAGCAGAAGAGTTGGAGAAGTATATCGCCATTAGAGAAGAGATGTATAAGAAAGCTAAAGAGTTCGATTCTAAGATCATTGGTTTTGAAACAACTATCAGGAGGCCCTACTTTCACGTACGACCTCTTAATATTGGGGAGCTTGAAAACTGGCATAATTATCTGGATTTTATAGAAAGAGAAGGCGACTTGAGCAAGGTAACCAAACTGCTTTCTTATAAGTGCACATTCTGTTCTAGTTTTTCCTGTTATAGATTGACTTACAGTAAGTAATATCTGCTGTATCATATGTCTTATAAGTGCACATCCTATTCTGTTGGATGAAGAAACTTTTTAATCGGGAATTGAACTGAGACTAAAGCATGGGCCTACAAATTGGTTTCTTGAGGCTAAGTACTTCAAACCCTAATTTGTCAGTGGACATTCTAGGTTTGTCATGTGTTTGCAAAAGTTTATTTTTAAAAGTGTGAATGTTATGAATGCAGGTATCTTCTAATCTTCATAATCACCTGTCTCAGGCAAGGAGGGTCCCTCTTTTCATATACGAGGGGCCTCTCTGTCCCGCCACGGAGTATTTCTAATGCTTTGAATTTTCTTTTTATGCTTCTCTTTCTTGATTTTTTTGCTTGCATTTACATTGTTGGACAATTTTTATGATGTGTTCTTTTCTTGTTTTGTTGTTTGTGGATCAGATTGTCAAGTTGTATGAGAGATGTGTCATTGCCTGTGCCAATTATCCCGAGTACTGGATACGCTATGTTTTGTGTATGGAAGCTAGGGAAAGTATGGATCTTGCAAATAATGTTCTCGCTCGGGCGAGCCAAGTTTTTGTCAAGGTAATTTCTTCCAAATGTCAAAGCTGTAGTTTTGGCAGCCATTTGCTGCTTTTTAGTGTTGTATTCATCTGTCCTGTTTTTAAGCTAAGTGAATTGGCAAGAAACACACATATATAATGGACTACTAGCCATAGGATAATAGTTCATGAAACTTGATATAGTGTGTCAATACACCATAGGTTGGTTTTTTGTTTACCTGATTGTAAATGGTGAAGGGGCATCTTATATTTTTGAGCTTTATGTTTATATGATTTCTTTAGGGTAAATGAAAGTTCAATTGTCTTTTATTTCCCCATCAGGGCCTTCGTTTAGCCGAGAATAGAAATCTCATGTACACCGCGTTGCCAATGACCCATTTCGTATAAGTGAAGGCGTATAAATGATTTTTTTTTTTTATATCGCCTGTCTTACACAAGACATATGATCCCTTTGAGGTTGTGACTAGCTAGTGATTCAATTACTCATTTCCTTTTCATGGAGGTATTGGTATTATGTCGTAGATCAGTTATCCTGAAAGCCTAAACTGCCAGGTCACATATACGAAGAGTATTTTCATATCACTGGCAAGCTTTTTGTTGTGGCCCATCAGTGAATGGTGTTTATTATATGTGCAATTTAGTCTTGATTTATAAAAAGCAATGTTCATGTATACTTTGTTTATAAGCATAATTAGCTCAATTGCATTTCTTGTGTGAGAGGCGTTTCCCTTTGTTTTGTTGCTGCTTTATCATTTTTTGCTTGTCATAATGCAGAGACAACCCGAGATACATCTTTTTTGCGCTCGGTTCAAGGAGCAGGCTGGAGATATTGTTGGCGCTAGAGCTGCGTATCAACTTGTTCACACTGAAATTTCTCCAGGTCTTCTTGAAGCAATAATCAGGCATGCCAATATGGAACATCGATTGGTATGCTGACTTACAAAAGCGTTAATAAACCGTGACTCAATTTTTGCTTAAATTTACAGCTTTTCCATTCCATGATATTGCAGGGGAAGTTGGAGGATGCCTTCTCTTTGTATGAACAGGCCATTGCCATTGAGAAAGGAAAAGAACATTCACAAACACTGCCAATGTTGTTTGCCCAGTATTCTCGATTTGTTTATTTGGTAGGTTTGGCAGCATATTTGTATTCTTCGCTTGTTATTCAGTCTTGTTTTGTTTCAGACATTTCCGCATTTGGCTATAGAACATGAAATAGGGACAGTTCTCTTAATTAACATTAGCTACTGTTTTTGTTGACCTGTCTGGATCTGGCCAAATGATGATTATTTCTGCGAGCCTCTCATTTATCCCAATAAATAGAGATGATTCTGGCTTTATATTATTTAACAATTTTTATATTATTTAACAATTTTCTAGCTTTATGCTTCAGTTGCTTTACTCGTTTGTGTGAAAATTGTCTAGTCTTGTTTTTGAAATATTCTTTCTCATTCTGTCATTAGGCTTCTGGGAATTATAGTTTTATGCTTCAGTTGCTATGCTTGTTTGTGTGAAAGTTGTCTTGTCTTGTTTTTGAAATATTCTTTCTTATTCTGTCATTAGGCTTCTGGGAATTCCGAAAAGGCTAGAGAGATTTTAGTTGGGGGACTTGAGAATGCTTCACTGTCAAAGCCACTACTTGAGGTTTGTCTCAACTTTCACTTTCTATTGATACTTAACTAATATTATGCACTTGGCAATTTTTCTCTTCGTCTTTCCAAATGTTTTTCATATCTAACGAGATTATTCAACTTCATCTCTAACGAGAGTATTTAACTTCATCTCTTACACAGGCTTTATTGCATTTTGAGGCTATTCAACCCCAGCCAAAGCGAGTTGACATTGACTTTCTAGAGTCACTGGTTGTAAAATTCATAACACCCAACCCAGAGAACCCTGGTGTAGCAAGTGCAACAGAGCGCGAGGAGCTTTCTAACATTTTTCTGGAGGTGATTTCTTATGAGGAGGATGTTCAATATTATATTTTATTAGTTTCCTTTAGTTAGCAGTATTATTAAGTTCTTACAAATTTTCTGTTGTTTTTTCAGTTTTTAAATCTCTTTGGAGATGTTCAATCGATCAAAAGGGTTGAGGATAGACATGCCAAACTGTTCTTGCCAAACAGGGGCTTGTCGGAGTTGAAAAAACGTCATGCAGAGGATTTCTTAGCCTCAGATAAAACAAAAGTGTCAAGAGCTTATTCTGCTCAATCACCTGCCCAATCTGTAGTGGGTGCATATCCAAATGGACCTAACCAATGGCCTAACTATGGAGTACAGCCTCAAACTTGGCCAGCAACGACTCAAGCACAAGGACAACAATGGCCTGCTGGCTACACCCAGCAGCAGGTAAATTGGTTTTTGCCCTCAACTATTTTTCTTGTGTGTTGCTTCCCTAGTTCAAATCCCAGGGTTGCATGGTGTTTGTCTATGACGTGGGCGAAATTGTTTTATAAACTAGTGAGTCTTTCTTGCCGGGTTGTGCAATATAAACTTTTTTTTGTTTTTGGAAATAGTGGTGTACACAGGTGTGTCATAACCTTAATCCATGAAGCATTATAATTTGAAAACACCATATTAGCCTTGTTGAAATGGAATTTGCGCCATATCTCATTGTAATGAGTTGATTACCCTTTTAGTTTATATTTGCCTTGAACCCATAAAGACATCTTCATGCATTTTTATCTACAGAAAATCATGTCATAAATGCTCTCCATTCATGCTATGCCCGACAGTTTCATGTTTTCTAGAGTTAAACATTTGTCGCACTTGTAGTGTATGTTTGACACTGTGTTTGTGCATTCTAGAATGGTGAGGTATTCCTGTTCTGAAATTATCCATTCTGATTGAATACAGGCTGCCGCTTCCTATGGGGCTTATGCTGGATATGGAGGCAACTATGCTAACCCACAATTGCCTGCATCAGTTCCACAAAGCACTGCTTATGGGGCCTATCCTCCTGCATATCCTGCACAGGTGAAAAGCTGTTTCAGCCCTTATGATTTTTGCAGATTATTGTTTGCCTGATATGTTTCAGCTCTATAGTTCTTTCTTGCGAGTGTTAATTACAATTTTGCTCTGTAGAACAAGAGAAAGCAAAAAAAGTAAGGGGAATCATGTGATGGTGTTTGTGTGTGTGTATAGCAAGCAAAAAGAATGTAAAATATTATCGAACATACCATTGTTTTATAAGGCCTTGGCTTTTTTTTTTTTTTTTTTTTTTTTGAGGGAACACGTGCCCAGCGTATCAGGATTTTCATTGAGTTGAGTTTCACCAGTTTAATAATTGCACTAATAGTACATAAGTTTGGTTTCATTTCTCAGTTGTATTTGACTTCTCTGACCTTTTGTTACTCTTTCCATTTCTATATTTCTGCTAAACCTGAGTACTGCGAATTAGATTAGTGTTGATTATTTTTTCTGAATTTCAGCCGGCACTGCCTCAACAAAATTATGCACAGCCTGTTGCAGCCCCGACACAGCAACCTGCAGCCGTTCCTCAGGCATATTATGGGAATTACTACTGA
Protein sequence

>MS.gene018645.t1

MGDSETMVAEASAAETYPSDAYNNTASNPVPKVGANVPAPAEAAGEAAAALPNSGYAFTYSGDGNAYTAGDPNSVLQAQFSATNESKQAAGVPNEDSSLVGKEALDSAMVSADHSSVNGSVDTVGLENGNASENVDGSADEKQLTDAYAALSAEEDRLWNIVTANSSDFTAWTSLIDETEKVAENNILKMRRVYDAFLAEFPLCYGYWKKYADHEARLGSADKVVEVYERAVQGVTYSVDMWLHYCIFAISTYGDPDTVRRLFERGLAYVGTDYLSFPLWDKYIEYEYMQQDWARLAMIYTRILENPNQQLDRYFNSFKELASNRPLSELRTADEAAAVAGVVSEGTDQGVEGEVHPDGADNSPKPASAVLTEAEELEKYIAIREEMYKKAKEFDSKIIGFETTIRRPYFHVRPLNIGELENWHNYLDFIEREGDLSKIVKLYERCVIACANYPEYWIRYVLCMEARESMDLANNVLARASQVFVKRQPEIHLFCARFKEQAGDIVGARAAYQLVHTEISPGLLEAIIRHANMEHRLGKLEDAFSLYEQAIAIEKGKEHSQTLPMLFAQYSRFVYLASGNSEKAREILVGGLENASLSKPLLEALLHFEAIQPQPKRVDIDFLESLVVKFITPNPENPGVASATEREELSNIFLEFLNLFGDVQSIKRVEDRHAKLFLPNRGLSELKKRHAEDFLASDKTKVSRAYSAQSPAQSVVGAYPNGPNQWPNYGVQPQTWPATTQAQGQQWPAGYTQQQAAASYGAYAGYGGNYANPQLPASVPQSTAYGAYPPAYPAQPALPQQNYAQPVAAPTQQPAAVPQAYYGNYY