AlfalfaGEDB | MS.gene011973

M. sativa cultivar XinJiangDaYe / MS.gene011973

KEGG: K11093
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF12220.11

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene011973.t1	RHN42128.1	100	163	0	0	1	163	1	163	1.10E-85	325.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene011973.t1	Q42404	80.7	161	31	0	3	163	6	166	4.5e-72	271.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene011973.t1	G7LEU2	100.0	163	0	0	1	163	1	163	7.9e-86	325.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene011973	MS.gene85823	PPI
MS.gene011973	MS.gene73728	PPI
MS.gene011973	MS.gene70370	PPI
MS.gene011973	MS.gene88797	PPI
MS.gene011973	MS.gene07236	PPI
MS.gene060523	MS.gene011973	PPI
MS.gene011973	MS.gene062632	PPI
MS.gene03425	MS.gene011973	PPI
MS.gene011973	MS.gene85836	PPI
MS.gene004992	MS.gene011973	PPI
MS.gene043455	MS.gene011973	PPI
MS.gene73717	MS.gene011973	PPI
MS.gene069779	MS.gene011973	PPI
MS.gene21679	MS.gene011973	PPI
MS.gene035199	MS.gene011973	PPI
MS.gene011973	MS.gene067297	PPI
MS.gene011973	MS.gene52921	PPI
MS.gene03436	MS.gene011973	PPI
MS.gene73715	MS.gene011973	PPI
MS.gene011973	MS.gene36138	PPI
MS.gene74239	MS.gene011973	PPI
MS.gene011973	MS.gene052391	PPI
MS.gene011973	MS.gene055778	PPI
MS.gene73714	MS.gene011973	PPI
MS.gene43805	MS.gene011973	PPI
MS.gene011973	MS.gene03434	PPI
MS.gene032656	MS.gene011973	PPI
MS.gene011973	MS.gene025726	PPI
MS.gene011973	MS.gene005567	PPI
MS.gene011973	MS.gene90884	PPI
MS.gene011973	MS.gene028438	PPI
MS.gene011973	MS.gene065825	PPI
MS.gene011973	MS.gene85830	PPI
MS.gene011973	MS.gene053079	PPI
MS.gene84735	MS.gene011973	PPI
MS.gene011973	MS.gene21951	PPI
MS.gene52910	MS.gene011973	PPI
MS.gene88866	MS.gene011973	PPI
MS.gene85832	MS.gene011973	PPI
MS.gene011973	MS.gene22969	PPI
MS.gene011973	MS.gene23430	PPI
MS.gene011973	MS.gene82806	PPI
MS.gene44906	MS.gene011973	PPI
MS.gene006089	MS.gene011973	PPI
MS.gene35022	MS.gene011973	PPI
MS.gene82776	MS.gene011973	PPI
MS.gene028868	MS.gene011973	PPI
MS.gene011973	MS.gene06988	PPI
MS.gene046159	MS.gene011973	PPI
MS.gene011973	MS.gene007200	PPI
MS.gene60746	MS.gene011973	PPI
MS.gene011973	MS.gene024701	PPI
MS.gene018645	MS.gene011973	PPI
MS.gene24692	MS.gene011973	PPI
MS.gene053099	MS.gene011973	PPI
MS.gene31434	MS.gene011973	PPI
MS.gene011973	MS.gene20758	PPI
MS.gene011973	MS.gene025725	PPI
MS.gene011973	MS.gene90116	PPI
MS.gene032681	MS.gene011973	PPI
MS.gene011973	MS.gene22289	PPI
MS.gene050303	MS.gene011973	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene011973.t1	MTR_8g077840	100.000	163	0	0	1	163	1	163	1.69e-117	341

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene011973.t1	AT3G50670	80.745	161	31	0	3	163	6	166	3.52e-95	274
MS.gene011973.t1	AT3G50670	80.745	161	31	0	3	163	6	166	2.73e-93	277

Find 0 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTTCGTATATTTTTTA+TGG	+	15144:60927-60946	MS.gene011973:intron	10.0%
!!	AAAAATCAACAAATTTCAAA+CGG	-	15144:61643-61662	None:intergenic	15.0%
!!	AATTAACTAATCTAATATCT+AGG	-	15144:60428-60447	None:intergenic	15.0%
!!	ATTAACTAATCTAATATCTA+GGG	-	15144:60427-60446	None:intergenic	15.0%
!!!	TTGTTTATGTTTTTTGTTTA+GGG	+	15144:60689-60708	MS.gene011973:intron	15.0%
!!	CGTTAATTATGAAGAAAATT+AGG	-	15144:60355-60374	None:intergenic	20.0%
!!	TTAAAAGATAAGAACATTAC+AGG	-	15144:60790-60809	None:intergenic	20.0%
!!	TTATATTCTCAACTAATATG+TGG	-	15144:61275-61294	None:intergenic	20.0%
!!!	AGAGTTTGAAGTTATTTTAT+GGG	+	15144:60952-60971	MS.gene011973:intron	20.0%
!!!	CTTGTTTATGTTTTTTGTTT+AGG	+	15144:60688-60707	MS.gene011973:intron	20.0%
!!!	GTTTGATTATTGAATTTGTT+AGG	+	15144:60607-60626	MS.gene011973:intron	20.0%
!!!	TTGAAATTTGTTGATTTTTG+TGG	+	15144:61644-61663	MS.gene011973:intron	20.0%
!!!	TTGTTGATTTTCAATAGTTA+GGG	+	15144:60387-60406	MS.gene011973:intron	20.0%
!!!	TTTGTTGATTTTCAATAGTT+AGG	+	15144:60386-60405	MS.gene011973:intron	20.0%
!	AAATTAACAACGAAAAGTGA+GGG	-	15144:60330-60349	None:intergenic	25.0%
!	AATAATCAAACACCTGTTAA+CGG	-	15144:60598-60617	None:intergenic	25.0%
!	ATGACCACAAAACATTTATT+CGG	-	15144:61237-61256	None:intergenic	25.0%
!	CAAAACATTTATTCGGATTT+CGG	-	15144:61230-61249	None:intergenic	25.0%
!	CATAATGATCCAAATATATC+AGG	+	15144:61714-61733	MS.gene011973:CDS	25.0%
!	CATATAGTTAATGTAGCAAT+CGG	+	15144:61423-61442	MS.gene011973:intron	25.0%
!	CTTCAAATTACTTAAACAAG+TGG	+	15144:61335-61354	MS.gene011973:intron	25.0%
!	GAAAATATAACTTACAAGTC+TGG	-	15144:61766-61785	None:intergenic	25.0%
!	GATATATTTGGATCATTATG+TGG	-	15144:61714-61733	None:intergenic	25.0%
!	TAAGTAATTTGAAGAGAAAC+TGG	-	15144:61329-61348	None:intergenic	25.0%
!!	AAATCCGAATAAATGTTTTG+TGG	+	15144:61230-61249	MS.gene011973:intron	25.0%
!!	TTAACGTAACTGTTTTCATA+AGG	-	15144:61042-61061	None:intergenic	25.0%
!!!	CAGAGTTTGAAGTTATTTTA+TGG	+	15144:60951-60970	MS.gene011973:intron	25.0%
!!!	TCTTTTAAGACTAATTATGC+TGG	+	15144:60802-60821	MS.gene011973:intron	25.0%
!!!	TTATGTTTTTTGTTTAGGGA+TGG	+	15144:60693-60712	MS.gene011973:intron	25.0%
	ACTCTTCACTATTGAAACAA+GGG	-	15144:61484-61503	None:intergenic	30.0%
	ATTAGTACCATACAAAATGC+AGG	-	15144:61190-61209	None:intergenic	30.0%
	CAAATTAACAACGAAAAGTG+AGG	-	15144:60331-60350	None:intergenic	30.0%
	GTGGTTATTATAATGTACAG+TGG	+	15144:61354-61373	MS.gene011973:intron	30.0%
	TACTCTTCACTATTGAAACA+AGG	-	15144:61485-61504	None:intergenic	30.0%
	TATGGATCTCCTGATATATT+TGG	-	15144:61726-61745	None:intergenic	30.0%
	TATGGTACTAATTCGTTCTT+AGG	+	15144:61198-61217	MS.gene011973:intron	30.0%
!	GAATAAATGTTTTGTGGTCA+TGG	+	15144:61236-61255	MS.gene011973:intron	30.0%
!	TCCATACAAGACTTTATTTG+TGG	+	15144:61740-61759	MS.gene011973:CDS	30.0%
!!!	CGTGTTTTTTTTTTTTGCAC+TGG	+	15144:60866-60885	MS.gene011973:intron	30.0%
	AAAAGCAATGAGACACAACA+TGG	-	15144:61009-61028	None:intergenic	35.0%
	AAAAGGGAGTTTGAGTCATA+TGG	+	15144:61879-61898	MS.gene011973:CDS	35.0%
	AAGCACAGTAGTTACTCTAT+TGG	-	15144:61788-61807	None:intergenic	35.0%
	AGTCAGTATGTACTATGAAG+CGG	-	15144:61390-61409	None:intergenic	35.0%
	AGTCATATGGTGCAATCAAA+CGG	+	15144:61892-61911	MS.gene011973:CDS	35.0%
	ATTACAGGAGTTTCAACTAC+TGG	-	15144:60775-60794	None:intergenic	35.0%
	TACTGAGAGCAGAATCAAAA+GGG	+	15144:61863-61882	MS.gene011973:CDS	35.0%
	TATGGGCAAGAATGTAAGAT+TGG	+	15144:60969-60988	MS.gene011973:intron	35.0%
	TTTGAAGAGAAACTGGAATC+TGG	-	15144:61322-61341	None:intergenic	35.0%
!	AAGAGTACACAAGTTAAGAC+TGG	+	15144:61559-61578	MS.gene011973:CDS	35.0%
!	GCCACAAATAAAGTCTTGTA+TGG	-	15144:61744-61763	None:intergenic	35.0%
!!	GTAATTGATTTGACGCAGAT+TGG	+	15144:60472-60491	MS.gene011973:intron	35.0%
!!	TCTATACCAGTTTGAGTTGA+AGG	-	15144:60295-60314	None:intergenic	35.0%
	AAATTCGCAGTCAATCCAGT+TGG	-	15144:60508-60527	None:intergenic	40.0%
	AATCAAACACCTGTTAACGG+AGG	-	15144:60595-60614	None:intergenic	40.0%
	ACACAAGTTAAGACTGGAGA+AGG	+	15144:61565-61584	MS.gene011973:CDS	40.0%
	CACAAGTTAAGACTGGAGAA+GGG	+	15144:61566-61585	MS.gene011973:CDS	40.0%
	CAGTAGTCTCATAACTCTGT+TGG	-	15144:61848-61867	None:intergenic	40.0%
	CTACTGAGAGCAGAATCAAA+AGG	+	15144:61862-61881	MS.gene011973:CDS	40.0%
	CTCATAACTCTGTTGGAAGA+TGG	-	15144:61841-61860	None:intergenic	40.0%
	GTGCAAGCACAATGTTTAAC+CGG	+	15144:61145-61164	MS.gene011973:intron	40.0%
!	TGATACGTTTTTGTGCACTC+AGG	+	15144:61523-61542	MS.gene011973:intron	40.0%
!!	GTGAGTAAATTTGCTGAACC+TGG	+	15144:60724-60743	MS.gene011973:CDS	40.0%
	ATTGGACAGAGTCATCCAAC+TGG	+	15144:60490-60509	MS.gene011973:CDS	45.0%
	CAACGAAAAGTGAGGGAAAG+AGG	-	15144:60323-60342	None:intergenic	45.0%
	CAACGTCCTTCAACTCAAAC+TGG	+	15144:60286-60305	MS.gene011973:CDS	45.0%
	CCATACAAAATGCAGGCACA+AGG	-	15144:61183-61202	None:intergenic	45.0%
	CGAAAATGTCCTCCGTTAAC+AGG	+	15144:60583-60602	MS.gene011973:CDS	45.0%
	GGTGGAGGTTTATACTCCAA+CGG	-	15144:60556-60575	None:intergenic	45.0%
	GTTGGAGTATAAACCTCCAC+CGG	+	15144:60555-60574	MS.gene011973:CDS	45.0%
	TGTTGAAGCTGTTTGAGCCT+AGG	+	15144:60527-60546	MS.gene011973:CDS	45.0%
!	AGCTTTGGTACGAGCTTGAA+CGG	-	15144:60255-60274	None:intergenic	45.0%
!	ATTTGCTGAACCTGGTGAAC+CGG	+	15144:60732-60751	MS.gene011973:CDS	45.0%
!	CCTTGTGCCTGCATTTTGTA+TGG	+	15144:61180-61199	MS.gene011973:intron	45.0%
!	GGAGGACATTTTCGTTTCTC+CGG	-	15144:60577-60596	None:intergenic	45.0%
!	TTTTGCACTGGACATGTCTG+TGG	+	15144:60878-60897	MS.gene011973:intron	45.0%
!!	CAGTTTGAGTTGAAGGACGT+TGG	-	15144:60288-60307	None:intergenic	45.0%
	CACAAGGAGTCAGAGATAGC+CGG	-	15144:61167-61186	None:intergenic	50.0%
	GGAGAATATTCCGGTTCACC+AGG	-	15144:60745-60764	None:intergenic	50.0%
	GGAGGTTTATACTCCAACGG+AGG	-	15144:60553-60572	None:intergenic	50.0%
	GGAGTTTCAACTACTGGCAC+AGG	-	15144:60769-60788	None:intergenic	50.0%
	GTACTATGAAGCGGCTACGT+CGG	-	15144:61381-61400	None:intergenic	50.0%
	GTGCACTCAGGCAGAAAGAA+GGG	+	15144:61535-61554	MS.gene011973:intron	50.0%
	GTTTCAACTACTGGCACAGG+CGG	-	15144:60766-60785	None:intergenic	50.0%
	TGTGCACTCAGGCAGAAAGA+AGG	+	15144:61534-61553	MS.gene011973:intron	50.0%
	TTATACTCCAACGGAGGCCT+AGG	-	15144:60547-60566	None:intergenic	50.0%
	TTTGGTACGAGCTTGAACGG+CGG	-	15144:60252-60271	None:intergenic	50.0%
!	CATTTTCGTTTCTCCGGTGG+AGG	-	15144:60571-60590	None:intergenic	50.0%
!	GGACATTTTCGTTTCTCCGG+TGG	-	15144:60574-60593	None:intergenic	50.0%
	GGCACAGGCGGAGAATATTC+CGG	-	15144:60754-60773	None:intergenic	55.0%
	GTTGGCGCGATTCTGAGCTT+TGG	-	15144:60270-60289	None:intergenic	55.0%
	TGCACTGGACATGTCTGTGG+CGG	+	15144:60881-60900	MS.gene011973:intron	55.0%
!!!	GCGTTTTGGTTGCGCAGAAG+AGG	-	15144:60230-60249	None:intergenic	55.0%
	GCACTGGACATGTCTGTGGC+GGG	+	15144:60882-60901	MS.gene011973:intron	60.0%
	GTTTGAGCCTAGGCCTCCGT+TGG	+	15144:60537-60556	MS.gene011973:CDS	60.0%
!	GAGCTTGAACGGCGGCGTTT+TGG	-	15144:60244-60263	None:intergenic	60.0%
	GGGAGCTGCGAAAGCTGCTG+AGG	+	15144:61586-61605	MS.gene011973:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
15144	gene	60218	61914	60218	ID=MS.gene011973
15144	mRNA	60218	61914	60218	ID=MS.gene011973.t1;Parent=MS.gene011973
15144	exon	60218	60307	60218	ID=MS.gene011973.t1.exon1;Parent=MS.gene011973.t1
15144	CDS	60218	60307	60218	ID=cds.MS.gene011973.t1;Parent=MS.gene011973.t1
15144	exon	60490	60604	60490	ID=MS.gene011973.t1.exon2;Parent=MS.gene011973.t1
15144	CDS	60490	60604	60490	ID=cds.MS.gene011973.t1;Parent=MS.gene011973.t1
15144	exon	60710	60789	60710	ID=MS.gene011973.t1.exon3;Parent=MS.gene011973.t1
15144	CDS	60710	60789	60710	ID=cds.MS.gene011973.t1;Parent=MS.gene011973.t1
15144	exon	61545	61620	61545	ID=MS.gene011973.t1.exon4;Parent=MS.gene011973.t1
15144	CDS	61545	61620	61545	ID=cds.MS.gene011973.t1;Parent=MS.gene011973.t1
15144	exon	61706	61770	61706	ID=MS.gene011973.t1.exon5;Parent=MS.gene011973.t1
15144	CDS	61706	61770	61706	ID=cds.MS.gene011973.t1;Parent=MS.gene011973.t1
15144	exon	61852	61914	61852	ID=MS.gene011973.t1.exon6;Parent=MS.gene011973.t1
15144	CDS	61852	61914	61852	ID=cds.MS.gene011973.t1;Parent=MS.gene011973.t1

Gene Sequence

>MS.gene011973

ATGGAAGATCCTCTTCTGCGCAACCAAAACGCCGCCGTTCAAGCTCGTACCAAAGCTCAGAATCGCGCCAACGTCCTTCAACTCAAACTGGTATAGATTCATCCTCTTTCCCTCACTTTTCGTTGTTAATTTGACCTAATTTTCTTCATAATTAACGAAACTGTTGTTTTTGTTGATTTTCAATAGTTAGGGTTTATTGTTCGTAACCCTAGATATTAGATTAGTTAATTTTTGTTGATTAATTTGATTTAATTGTAATTGATTTGACGCAGATTGGACAGAGTCATCCAACTGGATTGACTGCGAATTTGTTGAAGCTGTTTGAGCCTAGGCCTCCGTTGGAGTATAAACCTCCACCGGAGAAACGAAAATGTCCTCCGTTAACAGGTGTTTGATTATTGAATTTGTTAGGTTTTTGCGATAATTTATTGTTGATTGATGTTAATTATAGTTAATTATGCAATTAAGCTCTTGTTTATGTTTTTTGTTTAGGGATGGCGCAATTTGTGAGTAAATTTGCTGAACCTGGTGAACCGGAATATTCTCCGCCTGTGCCAGTAGTTGAAACTCCTGTAATGTTCTTATCTTTTAAGACTAATTATGCTGGAATTATTAGTTTTACTTGTGTTATGCATAGTTTCGTAATTGCGTGTTTTTTTTTTTTGCACTGGACATGTCTGTGGCGGGTTTGATTTGATGCAATGAGTTTATTTTTTCGTATATTTTTTATGGACAGAGTTTGAAGTTATTTTATGGGCAAGAATGTAAGATTGGTCTTTTGATGTGATCCATGTTGTGTCTCATTGCTTTTTAGAATATGTCCTTATGAAAACAGTTACGTTAACTAACTATTAGTTTGCAAAAAAATATGTGCTCAGAAGTGTTTTTTTACTGCTAAATTCTGCAGTTAAGTTTTACTCTAATATTGTGCAAGCACAATGTTTAACCGGCTATCTCTGACTCCTTGTGCCTGCATTTTGTATGGTACTAATTCGTTCTTAGGAAATATCCGAAATCCGAATAAATGTTTTGTGGTCATGGATAACAAAGAGCTCCACATATTAGTTGAGAATATAATTACTATTGCTTATTCAGTCTTTACCAGATTCCAGTTTCTCTTCAAATTACTTAAACAAGTGGTTATTATAATGTACAGTGGACCGACGTAGCCGCTTCATAGTACATACTGACTATCTATTGCTTTGCATATAGTTAATGTAGCAATCGGCATATAAAAGCAGTAAAGTAGTTTAAATGTCAAAGCCCTTGTTTCAATAGTGAAGAGTATAATAAAAGCATAATTGCTGATACGTTTTTGTGCACTCAGGCAGAAAGAAGGGCAAGAGTACACAAGTTAAGACTGGAGAAGGGAGCTGCGAAAGCTGCTGAGGAGCTTGAGAAATGTGATGACTCTTAAATATACCGTTTGAAATTTGTTGATTTTTGTGGATTATGTTATCTGAATATTTTTCATCTGTTTTGATGTAGATGATCCACATAATGATCCAAATATATCAGGAGATCCATACAAGACTTTATTTGTGGCCAGACTTGTAAGTTATATTTTCCAATAGAGTAACTACTGTGCTTTTTCATATTGCTTGTCATATCTTATTGATTCCATCTTCCAACAGAGTTATGAGACTACTGAGAGCAGAATCAAAAGGGAGTTTGAGTCATATGGTGCAATCAAACGG

Protein sequence

>MS.gene011973.t1

MEDPLLRNQNAAVQARTKAQNRANVLQLKLIGQSHPTGLTANLLKLFEPRPPLEYKPPPEKRKCPPLTGMAQFVSKFAEPGEPEYSPPVPVVETPAERRARVHKLRLEKGAAKAAEELEKYDPHNDPNISGDPYKTLFVARLSYETTESRIKREFESYGAIKR