Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035725.t1 | XP_003610112.1 | 100 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 2.80E-43 | 184.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035725.t1 | Q9SUM2 | 87.5 | 88 | 11 | 0 | 1 | 88 | 1 | 88 | 9.4e-40 | 163.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035725.t1 | G7JVF0 | 100.0 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 2.0e-43 | 184.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035725.t1 | MTR_4g128050 | 100.000 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 5.02e-61 | 180 |
| MS.gene035725.t1 | MTR_4g080300 | 45.455 | 66 | 36 | 0 | 8 | 73 | 15 | 80 | 1.64e-17 | 70.5 |
| MS.gene035725.t1 | MTR_7g114670 | 68.750 | 48 | 15 | 0 | 2 | 49 | 3 | 50 | 5.34e-17 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035725.t1 | AT4G30220 | 87.500 | 88 | 11 | 0 | 1 | 88 | 1 | 88 | 2.32e-53 | 161 |
| MS.gene035725.t1 | AT4G30220 | 85.227 | 88 | 13 | 0 | 1 | 88 | 9 | 96 | 4.60e-52 | 158 |
| MS.gene035725.t1 | AT2G14285 | 83.607 | 61 | 10 | 0 | 28 | 88 | 1 | 61 | 1.27e-32 | 108 |
| MS.gene035725.t1 | AT3G59810 | 44.928 | 69 | 38 | 0 | 8 | 76 | 15 | 83 | 1.32e-17 | 70.9 |
| MS.gene035725.t1 | AT3G59810 | 44.928 | 69 | 38 | 0 | 8 | 76 | 15 | 83 | 1.32e-17 | 70.9 |
| MS.gene035725.t1 | AT3G59810 | 44.928 | 69 | 38 | 0 | 8 | 76 | 15 | 83 | 1.32e-17 | 70.9 |
| MS.gene035725.t1 | AT2G43810 | 45.455 | 66 | 36 | 0 | 8 | 73 | 15 | 80 | 1.39e-17 | 70.9 |
| MS.gene035725.t1 | AT2G43810 | 45.455 | 66 | 36 | 0 | 8 | 73 | 15 | 80 | 1.39e-17 | 70.9 |
| MS.gene035725.t1 | AT2G43810 | 45.455 | 66 | 36 | 0 | 8 | 73 | 15 | 80 | 1.39e-17 | 70.9 |
Find 15 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTGAGTTTCACAATAAC+AGG | 0.298303 | 8.4:-6351502 | None:intergenic |
| CTGTTATTGTGAAACTCAAA+TGG | 0.394430 | 8.4:+6351503 | MS.gene035725:CDS |
| TGATTCCTACATGAACTTGC+AGG | 0.426591 | 8.4:+6351708 | MS.gene035725:CDS |
| TCTTCGATTTCTTCATCCTC+TGG | 0.426615 | 8.4:-6353483 | None:intergenic |
| TGTTATTGTGAAACTCAAAT+GGG | 0.470165 | 8.4:+6351504 | MS.gene035725:CDS |
| CTCAATGTACTCTTCAGTGT+TGG | 0.496731 | 8.4:-6351847 | None:intergenic |
| TGTGAAACTCAAATGGGGAA+TGG | 0.534967 | 8.4:+6351510 | MS.gene035725:CDS |
| AACACTGAAGAGTACATTGA+GGG | 0.587864 | 8.4:+6351849 | MS.gene035725:CDS |
| CTACATGCGCGGAGTACCAG+AGG | 0.623589 | 8.4:+6353467 | MS.gene035725:CDS |
| TTGTACCTGCAAGTTCATGT+AGG | 0.632824 | 8.4:-6351713 | None:intergenic |
| AACAATGTTCTCTACATGCG+CGG | 0.654078 | 8.4:+6353456 | MS.gene035725:CDS |
| TGTAGGAATCAACTGAAACA+AGG | 0.659877 | 8.4:-6351696 | None:intergenic |
| GTTATTGTGAAACTCAAATG+GGG | 0.661938 | 8.4:+6351505 | MS.gene035725:CDS |
| AAATGGGGAATGGAGTACAA+AGG | 0.669721 | 8.4:+6351520 | MS.gene035725:CDS |
| CAACACTGAAGAGTACATTG+AGG | 0.691161 | 8.4:+6351848 | MS.gene035725:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAATATTTTTTATTAAA+AGG | + | chr8.4:6353242-6353261 | MS.gene035725:intron | 0.0% |
| !! | AATTAGCAACATAATAATAA+AGG | - | chr8.4:6351551-6351570 | None:intergenic | 15.0% |
| !! | ATTAGCAACATAATAATAAA+GGG | - | chr8.4:6351550-6351569 | None:intergenic | 15.0% |
| !! | AGAAATAACACTATTAAAAG+AGG | - | chr8.4:6353389-6353408 | None:intergenic | 20.0% |
| !! | ATTAAAAGGTCTGAATATAT+TGG | + | chr8.4:6353256-6353275 | MS.gene035725:intron | 20.0% |
| !! | ATTCAATTGTTTGTATTTGT+AGG | + | chr8.4:6353430-6353449 | MS.gene035725:intron | 20.0% |
| !! | CATTCAATTAATTATGAATG+TGG | + | chr8.4:6352297-6352316 | MS.gene035725:intron | 20.0% |
| !!! | AATCTTAATATTTTTAGTGC+AGG | + | chr8.4:6353169-6353188 | MS.gene035725:intron | 20.0% |
| !!! | AATTTGCTTATAATTGACAA+CGG | + | chr8.4:6352371-6352390 | MS.gene035725:intron | 20.0% |
| !!! | AGTTTTGTTTATCTATCATT+TGG | + | chr8.4:6352030-6352049 | MS.gene035725:intron | 20.0% |
| !!! | ATATCTTTTAGTGATTATGT+GGG | - | chr8.4:6352595-6352614 | None:intergenic | 20.0% |
| !!! | TAAGCAAATTTTGACTTTTT+AGG | + | chr8.4:6352272-6352291 | MS.gene035725:intron | 20.0% |
| !!! | TTTTATCTTCATGAAAAGTT+TGG | + | chr8.4:6353349-6353368 | MS.gene035725:intron | 20.0% |
| ! | AAAGTATAGAAGATTCTGAT+CGG | - | chr8.4:6351318-6351337 | None:intergenic | 25.0% |
| ! | AAATTGTTCAAAAAAGGCTT+GGG | - | chr8.4:6351472-6351491 | None:intergenic | 25.0% |
| ! | AATCTTCTATACTTTCGATT+AGG | + | chr8.4:6351323-6351342 | MS.gene035725:intron | 25.0% |
| ! | ACACAAATCATAGAGAAAAT+TGG | - | chr8.4:6351375-6351394 | None:intergenic | 25.0% |
| ! | ATCATATAGCATATATCTTC+TGG | - | chr8.4:6352088-6352107 | None:intergenic | 25.0% |
| ! | CCAGTTAAATTGTTCAAAAA+AGG | - | chr8.4:6351478-6351497 | None:intergenic | 25.0% |
| ! | GCTAATATAAGCAAAAGAAT+TGG | - | chr8.4:6352193-6352212 | None:intergenic | 25.0% |
| ! | TAAATTGTTCAAAAAAGGCT+TGG | - | chr8.4:6351473-6351492 | None:intergenic | 25.0% |
| ! | TAAGGTTTGTAAGATTGTTT+GGG | - | chr8.4:6352911-6352930 | None:intergenic | 25.0% |
| ! | TGTTATTGTGAAACTCAAAT+GGG | + | chr8.4:6351504-6351523 | MS.gene035725:CDS | 25.0% |
| ! | TTTAAATTGATGTCGAAACT+GGG | + | chr8.4:6352002-6352021 | MS.gene035725:intron | 25.0% |
| !! | CATATCTTTTAGTGATTATG+TGG | - | chr8.4:6352596-6352615 | None:intergenic | 25.0% |
| !! | TTAGCTCTTCTAATATGATA+AGG | + | chr8.4:6352518-6352537 | MS.gene035725:intron | 25.0% |
| !! | TTTTAAATTGATGTCGAAAC+TGG | + | chr8.4:6352001-6352020 | MS.gene035725:intron | 25.0% |
| !!! | CCTTTTTTGAACAATTTAAC+TGG | + | chr8.4:6351475-6351494 | MS.gene035725:CDS | 25.0% |
| !!! | TCATCTATTTTATTTGCTAC+TGG | + | chr8.4:6351920-6351939 | MS.gene035725:intron | 25.0% |
| !!! | TTGTGTTGTTTTTATTTTGC+AGG | + | chr8.4:6351670-6351689 | MS.gene035725:intron | 25.0% |
| !!! | TTTTCTCTATGATTTGTGTA+TGG | + | chr8.4:6351376-6351395 | MS.gene035725:intron | 25.0% |
| ACAATTCTAGATACACTAGT+TGG | + | chr8.4:6351732-6351751 | MS.gene035725:intron | 30.0% | |
| ATGCTAATGGTATCATTAAC+GGG | - | chr8.4:6352630-6352649 | None:intergenic | 30.0% | |
| ATTATAAACCAACTAGATCG+AGG | - | chr8.4:6352151-6352170 | None:intergenic | 30.0% | |
| CAAAGAAGTAGCAAAAAGAA+TGG | - | chr8.4:6351770-6351789 | None:intergenic | 30.0% | |
| CTGTTATTGTGAAACTCAAA+TGG | + | chr8.4:6351503-6351522 | MS.gene035725:CDS | 30.0% | |
| GATGCTAATGGTATCATTAA+CGG | - | chr8.4:6352631-6352650 | None:intergenic | 30.0% | |
| GTAAGGTTTGTAAGATTGTT+TGG | - | chr8.4:6352912-6352931 | None:intergenic | 30.0% | |
| GTTATTGTGAAACTCAAATG+GGG | + | chr8.4:6351505-6351524 | MS.gene035725:CDS | 30.0% | |
| TAACAATGTAGCACATTTGT+TGG | - | chr8.4:6352501-6352520 | None:intergenic | 30.0% | |
| TACAACACACCATAAATACA+AGG | - | chr8.4:6353223-6353242 | None:intergenic | 30.0% | |
| TCAAATCAACAACAAACAAC+AGG | - | chr8.4:6352119-6352138 | None:intergenic | 30.0% | |
| TGCTTATAATTGACAACAGA+GGG | - | chr8.4:6352258-6352277 | None:intergenic | 30.0% | |
| TTATCCTTGCCTTGTATTTA+TGG | + | chr8.4:6353211-6353230 | MS.gene035725:intron | 30.0% | |
| TTGCTTATAATTGACAACAG+AGG | - | chr8.4:6352259-6352278 | None:intergenic | 30.0% | |
| ! | ACATTGAGGGAAATTTTACT+GGG | + | chr8.4:6351862-6351881 | MS.gene035725:CDS | 30.0% |
| ! | CATTTGAGTTTCACAATAAC+AGG | - | chr8.4:6351505-6351524 | None:intergenic | 30.0% |
| ! | TACATTGAGGGAAATTTTAC+TGG | + | chr8.4:6351861-6351880 | MS.gene035725:CDS | 30.0% |
| !! | ACTTAGGAGAGATTTTAATC+AGG | + | chr8.4:6351886-6351905 | MS.gene035725:CDS | 30.0% |
| !! | CATTCTTTTTGCTACTTCTT+TGG | + | chr8.4:6351768-6351787 | MS.gene035725:intron | 30.0% |
| AAAAAAGGCTTGGGATTAAC+AGG | - | chr8.4:6351463-6351482 | None:intergenic | 35.0% | |
| AAAATGTCTGACTTTGGCAT+TGG | + | chr8.4:6353301-6353320 | MS.gene035725:intron | 35.0% | |
| AACGAAGACATAACTAGAAG+TGG | - | chr8.4:6353037-6353056 | None:intergenic | 35.0% | |
| AACTCCCCAAATAAACATCA+AGG | + | chr8.4:6353092-6353111 | MS.gene035725:intron | 35.0% | |
| AATGATGCATCAGATGCTAA+TGG | - | chr8.4:6352643-6352662 | None:intergenic | 35.0% | |
| ACACCCTTGATGTTTATTTG+GGG | - | chr8.4:6353099-6353118 | None:intergenic | 35.0% | |
| ACGAAGACATAACTAGAAGT+GGG | - | chr8.4:6353036-6353055 | None:intergenic | 35.0% | |
| ACTCCCCAAATAAACATCAA+GGG | + | chr8.4:6353093-6353112 | MS.gene035725:intron | 35.0% | |
| ATGGACAAAATGTCTGACTT+TGG | + | chr8.4:6353295-6353314 | MS.gene035725:intron | 35.0% | |
| ATGTTGAGAAATCATGTGCT+AGG | - | chr8.4:6351960-6351979 | None:intergenic | 35.0% | |
| ATTAAAAGAGGTCACACAGT+CGG | - | chr8.4:6353377-6353396 | None:intergenic | 35.0% | |
| CACACCCTTGATGTTTATTT+GGG | - | chr8.4:6353100-6353119 | None:intergenic | 35.0% | |
| CAGCTGCTAAAACAATACAA+AGG | + | chr8.4:6352421-6352440 | MS.gene035725:intron | 35.0% | |
| CGGAAGGAAAAAGATACTTA+CGG | - | chr8.4:6351298-6351317 | None:intergenic | 35.0% | |
| GAGAAAATTGGATCGCATTA+AGG | - | chr8.4:6351363-6351382 | None:intergenic | 35.0% | |
| GCAAAAAGAATGGTCAAAAC+CGG | - | chr8.4:6351760-6351779 | None:intergenic | 35.0% | |
| GGTATCATTAACGGGATATT+GGG | - | chr8.4:6352622-6352641 | None:intergenic | 35.0% | |
| TAGCACATGATTTCTCAACA+TGG | + | chr8.4:6351959-6351978 | MS.gene035725:intron | 35.0% | |
| TAGTTATGTCTTCGTTAGCT+TGG | + | chr8.4:6353041-6353060 | MS.gene035725:intron | 35.0% | |
| TATAGAAGATTCTGATCGGA+AGG | - | chr8.4:6351314-6351333 | None:intergenic | 35.0% | |
| TCACACCCTTGATGTTTATT+TGG | - | chr8.4:6353101-6353120 | None:intergenic | 35.0% | |
| TCACCGTTAAAGCAATTGAA+GGG | + | chr8.4:6352999-6353018 | MS.gene035725:intron | 35.0% | |
| TGCTTATAATTGACAACGGA+GGG | + | chr8.4:6352375-6352394 | MS.gene035725:intron | 35.0% | |
| TGGTATCATTAACGGGATAT+TGG | - | chr8.4:6352623-6352642 | None:intergenic | 35.0% | |
| TGTAGGAATCAACTGAAACA+AGG | - | chr8.4:6351699-6351718 | None:intergenic | 35.0% | |
| TGTTTATCCAAACATGCACT+AGG | - | chr8.4:6352696-6352715 | None:intergenic | 35.0% | |
| TTCAATTGCTTTAACGGTGA+AGG | - | chr8.4:6352999-6353018 | None:intergenic | 35.0% | |
| TTCACCGTTAAAGCAATTGA+AGG | + | chr8.4:6352998-6353017 | MS.gene035725:intron | 35.0% | |
| TTGCTTATAATTGACAACGG+AGG | + | chr8.4:6352374-6352393 | MS.gene035725:intron | 35.0% | |
| ! | AACACTGAAGAGTACATTGA+GGG | + | chr8.4:6351849-6351868 | MS.gene035725:CDS | 35.0% |
| ! | AATAAACATCAAGGGTGTGA+TGG | + | chr8.4:6353101-6353120 | MS.gene035725:intron | 35.0% |
| ! | GGAAATTTTACTGGGAACTT+AGG | + | chr8.4:6351870-6351889 | MS.gene035725:CDS | 35.0% |
| AAATGGGGAATGGAGTACAA+AGG | + | chr8.4:6351520-6351539 | MS.gene035725:CDS | 40.0% | |
| AACAATGTTCTCTACATGCG+CGG | + | chr8.4:6353456-6353475 | MS.gene035725:CDS | 40.0% | |
| AATTGCTTTAACGGTGAAGG+AGG | - | chr8.4:6352996-6353015 | None:intergenic | 40.0% | |
| ATAGCGCAGTCTCAATTTAC+AGG | - | chr8.4:6352672-6352691 | None:intergenic | 40.0% | |
| CACACCATAAATACAAGGCA+AGG | - | chr8.4:6353218-6353237 | None:intergenic | 40.0% | |
| CGAAGACATAACTAGAAGTG+GGG | - | chr8.4:6353035-6353054 | None:intergenic | 40.0% | |
| CTCAATGTACTCTTCAGTGT+TGG | - | chr8.4:6351850-6351869 | None:intergenic | 40.0% | |
| TCTTCGATTTCTTCATCCTC+TGG | - | chr8.4:6353486-6353505 | None:intergenic | 40.0% | |
| TGAGCTTATCTACTGACGTA+AGG | - | chr8.4:6352929-6352948 | None:intergenic | 40.0% | |
| TGATTCCTACATGAACTTGC+AGG | + | chr8.4:6351708-6351727 | MS.gene035725:CDS | 40.0% | |
| TGGCCCTTCAATTGCTTTAA+CGG | - | chr8.4:6353005-6353024 | None:intergenic | 40.0% | |
| TGTGAAACTCAAATGGGGAA+TGG | + | chr8.4:6351510-6351529 | MS.gene035725:CDS | 40.0% | |
| TTGTACCTGCAAGTTCATGT+AGG | - | chr8.4:6351716-6351735 | None:intergenic | 40.0% | |
| ! | CAACACTGAAGAGTACATTG+AGG | + | chr8.4:6351848-6351867 | MS.gene035725:CDS | 40.0% |
| !! | AGGAAATGCTTCTGAAGCAA+TGG | + | chr8.4:6352538-6352557 | MS.gene035725:intron | 40.0% |
| !! | GGAAATGCTTCTGAAGCAAT+GGG | + | chr8.4:6352539-6352558 | MS.gene035725:intron | 40.0% |
| !! | TGGTGTTGTATTTCTGAGCA+TGG | + | chr8.4:6353276-6353295 | MS.gene035725:intron | 40.0% |
| !!! | ACTAGAAGTGGGGTTTTGTT+TGG | - | chr8.4:6353025-6353044 | None:intergenic | 40.0% |
| !!! | TGGGATCTTTTAGTTCCATC+TGG | - | chr8.4:6352576-6352595 | None:intergenic | 40.0% |
| CGCTATACCTAGTGCATGTT+TGG | + | chr8.4:6352686-6352705 | MS.gene035725:intron | 45.0% | |
| CTTGTTGGGGTAATACGACT+TGG | - | chr8.4:6352467-6352486 | None:intergenic | 45.0% | |
| TAAAGCGACCTCGATCTAGT+TGG | + | chr8.4:6352140-6352159 | MS.gene035725:intron | 45.0% | |
| TCTGTGAACTTCTCTGTAGC+TGG | + | chr8.4:6351824-6351843 | MS.gene035725:intron | 45.0% | |
| TGGCTAAGCATGCTCTTGTT+GGG | - | chr8.4:6352481-6352500 | None:intergenic | 45.0% | |
| TTGGCTAAGCATGCTCTTGT+TGG | - | chr8.4:6352482-6352501 | None:intergenic | 45.0% | |
| ! | AGTTCCATCTGGTTTGAGCA+AGG | - | chr8.4:6352565-6352584 | None:intergenic | 45.0% |
| ! | CTAGATACACTAGTTGGTGC+CGG | + | chr8.4:6351738-6351757 | MS.gene035725:intron | 45.0% |
| AAGGAGGAAGCTCTCCTAGA+AGG | - | chr8.4:6352980-6352999 | None:intergenic | 50.0% | |
| AGGAGGAAGCTCTCCTAGAA+GGG | - | chr8.4:6352979-6352998 | None:intergenic | 50.0% | |
| GGCTAAGCATGCTCTTGTTG+GGG | - | chr8.4:6352480-6352499 | None:intergenic | 50.0% | |
| AATAAGCCGCTGCCCCTTCT+AGG | + | chr8.4:6352963-6352982 | MS.gene035725:intron | 55.0% | |
| AGAAGGGGCAGCGGCTTATT+TGG | - | chr8.4:6352963-6352982 | None:intergenic | 55.0% | |
| GGAGGAAGCTCTCCTAGAAG+GGG | - | chr8.4:6352978-6352997 | None:intergenic | 55.0% | |
| TGGGCCTTGCTCAAACCAGA+TGG | + | chr8.4:6352558-6352577 | MS.gene035725:intron | 55.0% | |
| AGCTCTCCTAGAAGGGGCAG+CGG | - | chr8.4:6352972-6352991 | None:intergenic | 60.0% | |
| ! | CTACATGCGCGGAGTACCAG+AGG | + | chr8.4:6353467-6353486 | MS.gene035725:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 6351291 | 6353521 | 6351291 | ID=MS.gene035725 |
| chr8.4 | mRNA | 6351291 | 6353521 | 6351291 | ID=MS.gene035725.t1;Parent=MS.gene035725 |
| chr8.4 | exon | 6351291 | 6351296 | 6351291 | ID=MS.gene035725.t1.exon1;Parent=MS.gene035725.t1 |
| chr8.4 | CDS | 6351291 | 6351296 | 6351291 | ID=cds.MS.gene035725.t1;Parent=MS.gene035725.t1 |
| chr8.4 | exon | 6351454 | 6351541 | 6351454 | ID=MS.gene035725.t1.exon2;Parent=MS.gene035725.t1 |
| chr8.4 | CDS | 6351454 | 6351541 | 6351454 | ID=cds.MS.gene035725.t1;Parent=MS.gene035725.t1 |
| chr8.4 | exon | 6351692 | 6351729 | 6351692 | ID=MS.gene035725.t1.exon3;Parent=MS.gene035725.t1 |
| chr8.4 | CDS | 6351692 | 6351729 | 6351692 | ID=cds.MS.gene035725.t1;Parent=MS.gene035725.t1 |
| chr8.4 | exon | 6351843 | 6351907 | 6351843 | ID=MS.gene035725.t1.exon4;Parent=MS.gene035725.t1 |
| chr8.4 | CDS | 6351843 | 6351907 | 6351843 | ID=cds.MS.gene035725.t1;Parent=MS.gene035725.t1 |
| chr8.4 | exon | 6353452 | 6353521 | 6353452 | ID=MS.gene035725.t1.exon5;Parent=MS.gene035725.t1 |
| chr8.4 | CDS | 6353452 | 6353521 | 6353452 | ID=cds.MS.gene035725.t1;Parent=MS.gene035725.t1 |
| Gene Sequence |
| Protein sequence |
