Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047872.t1 | RHN42128.1 | 96.3 | 164 | 1 | 1 | 1 | 159 | 1 | 164 | 7.90E-81 | 309.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047872.t1 | Q42404 | 78.4 | 162 | 30 | 1 | 3 | 159 | 6 | 167 | 6.6e-68 | 258.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047872.t1 | G7LEU2 | 96.3 | 164 | 1 | 1 | 1 | 159 | 1 | 164 | 5.7e-81 | 309.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047872.t1 | MTR_8g077840 | 96.341 | 164 | 1 | 1 | 1 | 159 | 1 | 164 | 4.36e-111 | 325 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene047872.t1 | AT3G50670 | 78.395 | 162 | 30 | 1 | 3 | 159 | 6 | 167 | 5.80e-90 | 261 |
| MS.gene047872.t1 | AT3G50670 | 78.395 | 162 | 30 | 1 | 3 | 159 | 6 | 167 | 7.11e-88 | 263 |
Find 42 sgRNAs with CRISPR-Local
Find 97 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGGATCTCCTGATATATT+TGG | 0.152234 | 8.1:-30494290 | None:intergenic |
| GAGCTTGAACGGCGGCGTTT+TGG | 0.299733 | 8.1:-30492807 | None:intergenic |
| CGAAAATGTCCTCCGTTAAC+AGG | 0.366472 | 8.1:+30493149 | MS.gene047872:CDS |
| CATAATGATCCAAATATATC+AGG | 0.376380 | 8.1:+30494281 | MS.gene047872:CDS |
| CAACGTCCTTCAACTCAAAC+TGG | 0.421351 | 8.1:+30492852 | MS.gene047872:CDS |
| TCTATACCAGTTTGAGTTGA+AGG | 0.434011 | 8.1:-30492858 | None:intergenic |
| ATTTGCTGAACCTGGTGAAC+CGG | 0.436483 | 8.1:+30493298 | MS.gene047872:CDS |
| GGCACAGGCGGAGAATATTC+CGG | 0.440593 | 8.1:-30493317 | None:intergenic |
| GATATATTTGGATCATTATG+TGG | 0.448138 | 8.1:-30494278 | None:intergenic |
| GGCCACAAATAAGTCTTGTA+TGG | 0.455563 | 8.1:-30494308 | None:intergenic |
| AATAATCAAACACCTGTTAA+CGG | 0.456383 | 8.1:-30493161 | None:intergenic |
| AAAAGGGAGTTTGAGTCATA+TGG | 0.456755 | 8.1:+30494445 | MS.gene047872:CDS |
| AGTCATATGGTGCAATCAAA+CGG | 0.459733 | 8.1:+30494458 | MS.gene047872:CDS |
| TTATACTCCAACGGAGGCCT+AGG | 0.468257 | 8.1:-30493110 | None:intergenic |
| GTTGGCGCGATTCTGAGCTT+TGG | 0.469863 | 8.1:-30492833 | None:intergenic |
| TGTTGAAGCTGTTTGAGCCT+AGG | 0.485151 | 8.1:+30493093 | MS.gene047872:CDS |
| ATTACAGGAGTTTCAACTAC+TGG | 0.487503 | 8.1:-30493338 | None:intergenic |
| GGGAGCTGCGAAAGCTGCTG+AGG | 0.502637 | 8.1:+30494153 | MS.gene047872:CDS |
| GTCATATGGTGCAATCAAAC+GGG | 0.503431 | 8.1:+30494459 | MS.gene047872:CDS |
| CTACTGAGAGCAGAATCAAA+AGG | 0.508114 | 8.1:+30494428 | MS.gene047872:CDS |
| TGTGCACTCAGGCAGAAAGA+AGG | 0.516793 | 8.1:+30494101 | MS.gene047872:intron |
| ACACAAGTTAAGACTGGAGA+AGG | 0.520515 | 8.1:+30494132 | MS.gene047872:CDS |
| GTTTGAGCCTAGGCCTCCGT+TGG | 0.521330 | 8.1:+30493103 | MS.gene047872:CDS |
| GTGAGTAAATTTGCTGAACC+TGG | 0.524375 | 8.1:+30493290 | MS.gene047872:CDS |
| ATTGGACAGAGTCATCCAAC+TGG | 0.529631 | 8.1:+30493056 | MS.gene047872:CDS |
| GGAGAATATTCCGGTTCACC+AGG | 0.534830 | 8.1:-30493308 | None:intergenic |
| TACTGAGAGCAGAATCAAAA+GGG | 0.536291 | 8.1:+30494429 | MS.gene047872:CDS |
| GTGCACTCAGGCAGAAAGAA+GGG | 0.544215 | 8.1:+30494102 | MS.gene047872:intron |
| AGCTTTGGTACGAGCTTGAA+CGG | 0.573786 | 8.1:-30492818 | None:intergenic |
| GGAGTTTCAACTACTGGCAC+AGG | 0.580182 | 8.1:-30493332 | None:intergenic |
| AAGAGTACACAAGTTAAGAC+TGG | 0.602334 | 8.1:+30494126 | MS.gene047872:CDS |
| AAATTCGCAGTCAATCCAGT+TGG | 0.602338 | 8.1:-30493071 | None:intergenic |
| CAGTTTGAGTTGAAGGACGT+TGG | 0.604220 | 8.1:-30492851 | None:intergenic |
| GCGCAGAAGAGGATCTTCCA+TGG | 0.627859 | 8.1:-30492782 | None:intergenic |
| GTTGGAGTATAAACCTCCAC+CGG | 0.632767 | 8.1:+30493121 | MS.gene047872:CDS |
| GGTGGAGGTTTATACTCCAA+CGG | 0.633733 | 8.1:-30493119 | None:intergenic |
| TATGGTGCAATCAAACGGGT+AGG | 0.644989 | 8.1:+30494463 | MS.gene047872:CDS |
| GTTTCAACTACTGGCACAGG+CGG | 0.648421 | 8.1:-30493329 | None:intergenic |
| CACAAGTTAAGACTGGAGAA+GGG | 0.654118 | 8.1:+30494133 | MS.gene047872:CDS |
| TTTGGTACGAGCTTGAACGG+CGG | 0.655972 | 8.1:-30492815 | None:intergenic |
| GGAGGTTTATACTCCAACGG+AGG | 0.690191 | 8.1:-30493116 | None:intergenic |
| AATCAAACACCTGTTAACGG+AGG | 0.708779 | 8.1:-30493158 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTCGTATATTTTTTA+TGG | + | chr8.1:30493494-30493513 | MS.gene047872:intron | 10.0% |
| !! | AAAAATCAACAAATTTCAAA+CGG | - | chr8.1:30494210-30494229 | None:intergenic | 15.0% |
| !! | AATTAACTAATCTAATATCT+AGG | - | chr8.1:30492994-30493013 | None:intergenic | 15.0% |
| !! | ATTAACTAATCTAATATCTA+GGG | - | chr8.1:30492993-30493012 | None:intergenic | 15.0% |
| !!! | TTGTTTATGTTTTTTGTTTA+GGG | + | chr8.1:30493255-30493274 | MS.gene047872:intron | 15.0% |
| !! | CGTTAATTATGAAGAAAATT+AGG | - | chr8.1:30492921-30492940 | None:intergenic | 20.0% |
| !! | TTAAAAGATAAGAACATTAC+AGG | - | chr8.1:30493356-30493375 | None:intergenic | 20.0% |
| !! | TTATATTCTCAACTAATATG+TGG | - | chr8.1:30493842-30493861 | None:intergenic | 20.0% |
| !!! | AGAGTTTGAAGTTATTTTAT+GGG | + | chr8.1:30493519-30493538 | MS.gene047872:intron | 20.0% |
| !!! | CTTGTTTATGTTTTTTGTTT+AGG | + | chr8.1:30493254-30493273 | MS.gene047872:intron | 20.0% |
| !!! | GTTTGATTATTGAATTTGTT+AGG | + | chr8.1:30493173-30493192 | MS.gene047872:intron | 20.0% |
| !!! | TTGAAATTTGTTGATTTTTG+TGG | + | chr8.1:30494211-30494230 | MS.gene047872:intron | 20.0% |
| !!! | TTGTTGATTTTCAATAGTTA+GGG | + | chr8.1:30492953-30492972 | MS.gene047872:intron | 20.0% |
| !!! | TTTGTTGATTTTCAATAGTT+AGG | + | chr8.1:30492952-30492971 | MS.gene047872:intron | 20.0% |
| ! | AAATTAACAACGAAAAGTGA+GGG | - | chr8.1:30492896-30492915 | None:intergenic | 25.0% |
| ! | AATAATCAAACACCTGTTAA+CGG | - | chr8.1:30493164-30493183 | None:intergenic | 25.0% |
| ! | ATGACCACAAAACATTTATT+CGG | - | chr8.1:30493804-30493823 | None:intergenic | 25.0% |
| ! | CAAAACATTTATTCGGATTT+CGG | - | chr8.1:30493797-30493816 | None:intergenic | 25.0% |
| ! | CATAATGATCCAAATATATC+AGG | + | chr8.1:30494281-30494300 | MS.gene047872:CDS | 25.0% |
| ! | CATATAGTTAATGTAGCAAT+CGG | + | chr8.1:30493990-30494009 | MS.gene047872:intron | 25.0% |
| ! | CTTCAAATTACTTAAACAAG+TGG | + | chr8.1:30493902-30493921 | MS.gene047872:intron | 25.0% |
| ! | GAAAATATAACTTACAAGTC+TGG | - | chr8.1:30494332-30494351 | None:intergenic | 25.0% |
| ! | GATATATTTGGATCATTATG+TGG | - | chr8.1:30494281-30494300 | None:intergenic | 25.0% |
| ! | TAAGTAATTTGAAGAGAAAC+TGG | - | chr8.1:30493896-30493915 | None:intergenic | 25.0% |
| !! | AAATCCGAATAAATGTTTTG+TGG | + | chr8.1:30493797-30493816 | MS.gene047872:intron | 25.0% |
| !! | TTAACGTAACTGTTTTCATA+AGG | - | chr8.1:30493609-30493628 | None:intergenic | 25.0% |
| !!! | CAGAGTTTGAAGTTATTTTA+TGG | + | chr8.1:30493518-30493537 | MS.gene047872:intron | 25.0% |
| !!! | GTGTTTTTTTTTTTTTGCAC+TGG | + | chr8.1:30493433-30493452 | MS.gene047872:intron | 25.0% |
| !!! | TCTTTTAAGACTAATTATGC+TGG | + | chr8.1:30493368-30493387 | MS.gene047872:intron | 25.0% |
| !!! | TTATGTTTTTTGTTTAGGGA+TGG | + | chr8.1:30493259-30493278 | MS.gene047872:intron | 25.0% |
| ACTCTTCACTATTGAAACAA+GGG | - | chr8.1:30494051-30494070 | None:intergenic | 30.0% | |
| ATCCATACAAGACTTATTTG+TGG | + | chr8.1:30494306-30494325 | MS.gene047872:intron | 30.0% | |
| ATTAGTACCATACAAAATGC+AGG | - | chr8.1:30493757-30493776 | None:intergenic | 30.0% | |
| CAAATTAACAACGAAAAGTG+AGG | - | chr8.1:30492897-30492916 | None:intergenic | 30.0% | |
| GTGGTTATTATAATGTACAG+TGG | + | chr8.1:30493921-30493940 | MS.gene047872:intron | 30.0% | |
| TACTCTTCACTATTGAAACA+AGG | - | chr8.1:30494052-30494071 | None:intergenic | 30.0% | |
| TATGGATCTCCTGATATATT+TGG | - | chr8.1:30494293-30494312 | None:intergenic | 30.0% | |
| TATGGTACTAATTCGTTCTT+AGG | + | chr8.1:30493765-30493784 | MS.gene047872:intron | 30.0% | |
| ! | GAATAAATGTTTTGTGGTCA+TGG | + | chr8.1:30493803-30493822 | MS.gene047872:intron | 30.0% |
| AAAAGCAATGAGACACAACA+TGG | - | chr8.1:30493576-30493595 | None:intergenic | 35.0% | |
| AAAAGGGAGTTTGAGTCATA+TGG | + | chr8.1:30494445-30494464 | MS.gene047872:CDS | 35.0% | |
| AAGCACAGTAGTTACTCTAT+TGG | - | chr8.1:30494354-30494373 | None:intergenic | 35.0% | |
| AGTCAGTATGTACTATGAAG+CGG | - | chr8.1:30493957-30493976 | None:intergenic | 35.0% | |
| AGTCATATGGTGCAATCAAA+CGG | + | chr8.1:30494458-30494477 | MS.gene047872:CDS | 35.0% | |
| ATTACAGGAGTTTCAACTAC+TGG | - | chr8.1:30493341-30493360 | None:intergenic | 35.0% | |
| TACTGAGAGCAGAATCAAAA+GGG | + | chr8.1:30494429-30494448 | MS.gene047872:CDS | 35.0% | |
| TATGGGCAAGAATGTAAGAT+TGG | + | chr8.1:30493536-30493555 | MS.gene047872:intron | 35.0% | |
| TTTGAAGAGAAACTGGAATC+TGG | - | chr8.1:30493889-30493908 | None:intergenic | 35.0% | |
| ! | AAGAGTACACAAGTTAAGAC+TGG | + | chr8.1:30494126-30494145 | MS.gene047872:CDS | 35.0% |
| !! | GTAATTGATTTGACGCAGAT+TGG | + | chr8.1:30493038-30493057 | MS.gene047872:intron | 35.0% |
| !! | TCTATACCAGTTTGAGTTGA+AGG | - | chr8.1:30492861-30492880 | None:intergenic | 35.0% |
| AAATTCGCAGTCAATCCAGT+TGG | - | chr8.1:30493074-30493093 | None:intergenic | 40.0% | |
| AATCAAACACCTGTTAACGG+AGG | - | chr8.1:30493161-30493180 | None:intergenic | 40.0% | |
| ACACAAGTTAAGACTGGAGA+AGG | + | chr8.1:30494132-30494151 | MS.gene047872:CDS | 40.0% | |
| CACAAGTTAAGACTGGAGAA+GGG | + | chr8.1:30494133-30494152 | MS.gene047872:CDS | 40.0% | |
| CAGTAGTCTCATAACTCTGT+TGG | - | chr8.1:30494414-30494433 | None:intergenic | 40.0% | |
| CTACTGAGAGCAGAATCAAA+AGG | + | chr8.1:30494428-30494447 | MS.gene047872:CDS | 40.0% | |
| CTCATAACTCTGTTGGAAGA+TGG | - | chr8.1:30494407-30494426 | None:intergenic | 40.0% | |
| GTCATATGGTGCAATCAAAC+GGG | + | chr8.1:30494459-30494478 | MS.gene047872:CDS | 40.0% | |
| GTGCAAGCACAATGTTTAAC+CGG | + | chr8.1:30493712-30493731 | MS.gene047872:intron | 40.0% | |
| ! | GGCCACAAATAAGTCTTGTA+TGG | - | chr8.1:30494311-30494330 | None:intergenic | 40.0% |
| ! | TGATACGTTTTTGTGCACTC+AGG | + | chr8.1:30494090-30494109 | MS.gene047872:intron | 40.0% |
| !! | GTGAGTAAATTTGCTGAACC+TGG | + | chr8.1:30493290-30493309 | MS.gene047872:CDS | 40.0% |
| ATTGGACAGAGTCATCCAAC+TGG | + | chr8.1:30493056-30493075 | MS.gene047872:CDS | 45.0% | |
| CAACGAAAAGTGAGGGAAAG+AGG | - | chr8.1:30492889-30492908 | None:intergenic | 45.0% | |
| CAACGTCCTTCAACTCAAAC+TGG | + | chr8.1:30492852-30492871 | MS.gene047872:CDS | 45.0% | |
| CCATACAAAATGCAGGCACA+AGG | - | chr8.1:30493750-30493769 | None:intergenic | 45.0% | |
| CGAAAATGTCCTCCGTTAAC+AGG | + | chr8.1:30493149-30493168 | MS.gene047872:CDS | 45.0% | |
| GGTGGAGGTTTATACTCCAA+CGG | - | chr8.1:30493122-30493141 | None:intergenic | 45.0% | |
| GTTGGAGTATAAACCTCCAC+CGG | + | chr8.1:30493121-30493140 | MS.gene047872:CDS | 45.0% | |
| TGTTGAAGCTGTTTGAGCCT+AGG | + | chr8.1:30493093-30493112 | MS.gene047872:CDS | 45.0% | |
| ! | AGCTTTGGTACGAGCTTGAA+CGG | - | chr8.1:30492821-30492840 | None:intergenic | 45.0% |
| ! | ATTTGCTGAACCTGGTGAAC+CGG | + | chr8.1:30493298-30493317 | MS.gene047872:CDS | 45.0% |
| ! | CCTTGTGCCTGCATTTTGTA+TGG | + | chr8.1:30493747-30493766 | MS.gene047872:intron | 45.0% |
| ! | GGAGGACATTTTCGTTTCTC+CGG | - | chr8.1:30493143-30493162 | None:intergenic | 45.0% |
| ! | TTTTGCACTGGACATGTCTG+TGG | + | chr8.1:30493445-30493464 | MS.gene047872:intron | 45.0% |
| !! | CAGTTTGAGTTGAAGGACGT+TGG | - | chr8.1:30492854-30492873 | None:intergenic | 45.0% |
| CACAAGGAGTCAGAGATAGC+CGG | - | chr8.1:30493734-30493753 | None:intergenic | 50.0% | |
| GGAGAATATTCCGGTTCACC+AGG | - | chr8.1:30493311-30493330 | None:intergenic | 50.0% | |
| GGAGGTTTATACTCCAACGG+AGG | - | chr8.1:30493119-30493138 | None:intergenic | 50.0% | |
| GGAGTTTCAACTACTGGCAC+AGG | - | chr8.1:30493335-30493354 | None:intergenic | 50.0% | |
| GTACTATGAAGCGGCTACGT+CGG | - | chr8.1:30493948-30493967 | None:intergenic | 50.0% | |
| GTGCACTCAGGCAGAAAGAA+GGG | + | chr8.1:30494102-30494121 | MS.gene047872:intron | 50.0% | |
| GTTTCAACTACTGGCACAGG+CGG | - | chr8.1:30493332-30493351 | None:intergenic | 50.0% | |
| TGTGCACTCAGGCAGAAAGA+AGG | + | chr8.1:30494101-30494120 | MS.gene047872:intron | 50.0% | |
| TTATACTCCAACGGAGGCCT+AGG | - | chr8.1:30493113-30493132 | None:intergenic | 50.0% | |
| TTTGGTACGAGCTTGAACGG+CGG | - | chr8.1:30492818-30492837 | None:intergenic | 50.0% | |
| ! | CATTTTCGTTTCTCCGGTGG+AGG | - | chr8.1:30493137-30493156 | None:intergenic | 50.0% |
| ! | GGACATTTTCGTTTCTCCGG+TGG | - | chr8.1:30493140-30493159 | None:intergenic | 50.0% |
| GGCACAGGCGGAGAATATTC+CGG | - | chr8.1:30493320-30493339 | None:intergenic | 55.0% | |
| GTTGGCGCGATTCTGAGCTT+TGG | - | chr8.1:30492836-30492855 | None:intergenic | 55.0% | |
| TGCACTGGACATGTCTGTGG+CGG | + | chr8.1:30493448-30493467 | MS.gene047872:intron | 55.0% | |
| !!! | GCGTTTTGGTTGCGCAGAAG+AGG | - | chr8.1:30492796-30492815 | None:intergenic | 55.0% |
| GCACTGGACATGTCTGTGGC+GGG | + | chr8.1:30493449-30493468 | MS.gene047872:intron | 60.0% | |
| GTTTGAGCCTAGGCCTCCGT+TGG | + | chr8.1:30493103-30493122 | MS.gene047872:CDS | 60.0% | |
| ! | GAGCTTGAACGGCGGCGTTT+TGG | - | chr8.1:30492810-30492829 | None:intergenic | 60.0% |
| GGGAGCTGCGAAAGCTGCTG+AGG | + | chr8.1:30494153-30494172 | MS.gene047872:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 30492784 | 30494484 | 30492784 | ID=MS.gene047872 |
| chr8.1 | mRNA | 30492784 | 30494484 | 30492784 | ID=MS.gene047872.t1;Parent=MS.gene047872 |
| chr8.1 | exon | 30492784 | 30492873 | 30492784 | ID=MS.gene047872.t1.exon1;Parent=MS.gene047872.t1 |
| chr8.1 | CDS | 30492784 | 30492873 | 30492784 | ID=cds.MS.gene047872.t1;Parent=MS.gene047872.t1 |
| chr8.1 | exon | 30493056 | 30493170 | 30493056 | ID=MS.gene047872.t1.exon2;Parent=MS.gene047872.t1 |
| chr8.1 | CDS | 30493056 | 30493170 | 30493056 | ID=cds.MS.gene047872.t1;Parent=MS.gene047872.t1 |
| chr8.1 | exon | 30493276 | 30493355 | 30493276 | ID=MS.gene047872.t1.exon3;Parent=MS.gene047872.t1 |
| chr8.1 | CDS | 30493276 | 30493355 | 30493276 | ID=cds.MS.gene047872.t1;Parent=MS.gene047872.t1 |
| chr8.1 | exon | 30494112 | 30494187 | 30494112 | ID=MS.gene047872.t1.exon4;Parent=MS.gene047872.t1 |
| chr8.1 | CDS | 30494112 | 30494187 | 30494112 | ID=cds.MS.gene047872.t1;Parent=MS.gene047872.t1 |
| chr8.1 | exon | 30494273 | 30494324 | 30494273 | ID=MS.gene047872.t1.exon5;Parent=MS.gene047872.t1 |
| chr8.1 | CDS | 30494273 | 30494324 | 30494273 | ID=cds.MS.gene047872.t1;Parent=MS.gene047872.t1 |
| chr8.1 | exon | 30494420 | 30494484 | 30494420 | ID=MS.gene047872.t1.exon6;Parent=MS.gene047872.t1 |
| chr8.1 | CDS | 30494420 | 30494484 | 30494420 | ID=cds.MS.gene047872.t1;Parent=MS.gene047872.t1 |
| Gene Sequence |
| Protein sequence |
