Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068867.t1 | XP_003610112.1 | 100 | 86 | 0 | 0 | 1 | 86 | 3 | 88 | 3.00E-42 | 180.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068867.t1 | Q9SUM2 | 87.2 | 86 | 11 | 0 | 1 | 86 | 3 | 88 | 1.0e-38 | 160.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068867.t1 | G7JVF0 | 100.0 | 86 | 0 | 0 | 1 | 86 | 3 | 88 | 2.2e-42 | 180.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068867.t1 | MTR_4g128050 | 100.000 | 86 | 0 | 0 | 1 | 86 | 3 | 88 | 2.18e-59 | 176 |
| MS.gene068867.t1 | MTR_4g080300 | 45.455 | 66 | 36 | 0 | 6 | 71 | 15 | 80 | 1.73e-17 | 70.5 |
| MS.gene068867.t1 | MTR_7g114670 | 74.419 | 43 | 11 | 0 | 5 | 47 | 8 | 50 | 8.48e-17 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene068867.t1 | AT4G30220 | 87.209 | 86 | 11 | 0 | 1 | 86 | 3 | 88 | 1.04e-51 | 157 |
| MS.gene068867.t1 | AT4G30220 | 87.209 | 86 | 11 | 0 | 1 | 86 | 11 | 96 | 1.06e-51 | 157 |
| MS.gene068867.t1 | AT2G14285 | 83.607 | 61 | 10 | 0 | 26 | 86 | 1 | 61 | 1.43e-32 | 107 |
| MS.gene068867.t1 | AT3G59810 | 44.928 | 69 | 38 | 0 | 6 | 74 | 15 | 83 | 1.51e-17 | 70.9 |
| MS.gene068867.t1 | AT3G59810 | 44.928 | 69 | 38 | 0 | 6 | 74 | 15 | 83 | 1.51e-17 | 70.9 |
| MS.gene068867.t1 | AT3G59810 | 44.928 | 69 | 38 | 0 | 6 | 74 | 15 | 83 | 1.51e-17 | 70.9 |
| MS.gene068867.t1 | AT2G43810 | 45.455 | 66 | 36 | 0 | 6 | 71 | 15 | 80 | 1.56e-17 | 70.9 |
| MS.gene068867.t1 | AT2G43810 | 45.455 | 66 | 36 | 0 | 6 | 71 | 15 | 80 | 1.56e-17 | 70.9 |
| MS.gene068867.t1 | AT2G43810 | 45.455 | 66 | 36 | 0 | 6 | 71 | 15 | 80 | 1.56e-17 | 70.9 |
Find 15 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTGAGTTTCACAATAAC+AGG | 0.298303 | 8.4:-6373883 | None:intergenic |
| CTGTTATTGTGAAACTCAAA+TGG | 0.394430 | 8.4:+6373884 | MS.gene068867:CDS |
| TGATTCCTACATGAACTTGC+AGG | 0.426591 | 8.4:+6374089 | MS.gene068867:CDS |
| TCTTCGATTTCTTCATCCTC+TGG | 0.426615 | 8.4:-6375864 | None:intergenic |
| TGTTATTGTGAAACTCAAAT+GGG | 0.470165 | 8.4:+6373885 | MS.gene068867:CDS |
| CTCAATGTACTCTTCAGTGT+TGG | 0.496731 | 8.4:-6374228 | None:intergenic |
| TGTGAAACTCAAATGGGGAA+TGG | 0.534967 | 8.4:+6373891 | MS.gene068867:CDS |
| AACACTGAAGAGTACATTGA+GGG | 0.587864 | 8.4:+6374230 | MS.gene068867:CDS |
| CTACATGCGCGGAGTACCAG+AGG | 0.623589 | 8.4:+6375848 | MS.gene068867:CDS |
| TTGTACCTGCAAGTTCATGT+AGG | 0.632824 | 8.4:-6374094 | None:intergenic |
| AACAATGTTCTCTACATGCG+CGG | 0.654078 | 8.4:+6375837 | MS.gene068867:CDS |
| TGTAGGAATCAACTGAAACA+AGG | 0.659877 | 8.4:-6374077 | None:intergenic |
| GTTATTGTGAAACTCAAATG+GGG | 0.661938 | 8.4:+6373886 | MS.gene068867:CDS |
| AAATGGGGAATGGAGTACAA+AGG | 0.669721 | 8.4:+6373901 | MS.gene068867:CDS |
| CAACACTGAAGAGTACATTG+AGG | 0.691161 | 8.4:+6374229 | MS.gene068867:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAATATTTTTTATTAAA+AGG | + | chr8.4:6375623-6375642 | MS.gene068867:intron | 0.0% |
| !! | AATTAGCAACATAATAATAA+AGG | - | chr8.4:6373932-6373951 | None:intergenic | 15.0% |
| !! | ATTAGCAACATAATAATAAA+GGG | - | chr8.4:6373931-6373950 | None:intergenic | 15.0% |
| !! | AGAAATAACACTATTAAAAG+AGG | - | chr8.4:6375770-6375789 | None:intergenic | 20.0% |
| !! | ATTAAAAGGTCTGAATATAT+TGG | + | chr8.4:6375637-6375656 | MS.gene068867:intron | 20.0% |
| !! | ATTCAATTGTTTGTATTTGT+AGG | + | chr8.4:6375811-6375830 | MS.gene068867:intron | 20.0% |
| !! | CATTCAATTAATTATGAATG+TGG | + | chr8.4:6374678-6374697 | MS.gene068867:intron | 20.0% |
| !!! | AATCTTAATATTTTTAGTGC+AGG | + | chr8.4:6375550-6375569 | MS.gene068867:intron | 20.0% |
| !!! | AATTTGCTTATAATTGACAA+CGG | + | chr8.4:6374752-6374771 | MS.gene068867:intron | 20.0% |
| !!! | AGTTTTGTTTATCTATCATT+TGG | + | chr8.4:6374411-6374430 | MS.gene068867:intron | 20.0% |
| !!! | ATATCTTTTAGTGATTATGT+GGG | - | chr8.4:6374976-6374995 | None:intergenic | 20.0% |
| !!! | TAAGCAAATTTTGACTTTTT+AGG | + | chr8.4:6374653-6374672 | MS.gene068867:intron | 20.0% |
| !!! | TTTTATCTTCATGAAAAGTT+TGG | + | chr8.4:6375730-6375749 | MS.gene068867:intron | 20.0% |
| ! | AAATTGTTCAAAAAAGGCTT+GGG | - | chr8.4:6373853-6373872 | None:intergenic | 25.0% |
| ! | ATCATATAGCATATATCTTC+TGG | - | chr8.4:6374469-6374488 | None:intergenic | 25.0% |
| ! | CCAGTTAAATTGTTCAAAAA+AGG | - | chr8.4:6373859-6373878 | None:intergenic | 25.0% |
| ! | GCTAATATAAGCAAAAGAAT+TGG | - | chr8.4:6374574-6374593 | None:intergenic | 25.0% |
| ! | TAAATTGTTCAAAAAAGGCT+TGG | - | chr8.4:6373854-6373873 | None:intergenic | 25.0% |
| ! | TAAGGTTTGTAAGATTGTTT+GGG | - | chr8.4:6375292-6375311 | None:intergenic | 25.0% |
| ! | TGTTATTGTGAAACTCAAAT+GGG | + | chr8.4:6373885-6373904 | MS.gene068867:CDS | 25.0% |
| ! | TTTAAATTGATGTCGAAACT+GGG | + | chr8.4:6374383-6374402 | MS.gene068867:intron | 25.0% |
| !! | CATATCTTTTAGTGATTATG+TGG | - | chr8.4:6374977-6374996 | None:intergenic | 25.0% |
| !! | TTAGCTCTTCTAATATGATA+AGG | + | chr8.4:6374899-6374918 | MS.gene068867:intron | 25.0% |
| !! | TTTTAAATTGATGTCGAAAC+TGG | + | chr8.4:6374382-6374401 | MS.gene068867:intron | 25.0% |
| !!! | CCTTTTTTGAACAATTTAAC+TGG | + | chr8.4:6373856-6373875 | MS.gene068867:CDS | 25.0% |
| !!! | TCATCTATTTTATTTGCTAC+TGG | + | chr8.4:6374301-6374320 | MS.gene068867:intron | 25.0% |
| !!! | TTGTGTTGTTTTTATTTTGC+AGG | + | chr8.4:6374051-6374070 | MS.gene068867:intron | 25.0% |
| ACAATTCTAGATACACTAGT+TGG | + | chr8.4:6374113-6374132 | MS.gene068867:intron | 30.0% | |
| ATGCTAATGGTATCATTAAC+GGG | - | chr8.4:6375011-6375030 | None:intergenic | 30.0% | |
| ATTATAAACCAACTAGATCG+AGG | - | chr8.4:6374532-6374551 | None:intergenic | 30.0% | |
| CAAAGAAGTAGCAAAAAGAA+TGG | - | chr8.4:6374151-6374170 | None:intergenic | 30.0% | |
| CTGTTATTGTGAAACTCAAA+TGG | + | chr8.4:6373884-6373903 | MS.gene068867:CDS | 30.0% | |
| GATGCTAATGGTATCATTAA+CGG | - | chr8.4:6375012-6375031 | None:intergenic | 30.0% | |
| GTAAGGTTTGTAAGATTGTT+TGG | - | chr8.4:6375293-6375312 | None:intergenic | 30.0% | |
| GTTATTGTGAAACTCAAATG+GGG | + | chr8.4:6373886-6373905 | MS.gene068867:CDS | 30.0% | |
| TAACAATGTAGCACATTTGT+TGG | - | chr8.4:6374882-6374901 | None:intergenic | 30.0% | |
| TACAACACACCATAAATACA+AGG | - | chr8.4:6375604-6375623 | None:intergenic | 30.0% | |
| TCAAATCAACAACAAACAAC+AGG | - | chr8.4:6374500-6374519 | None:intergenic | 30.0% | |
| TGCTTATAATTGACAACAGA+GGG | - | chr8.4:6374639-6374658 | None:intergenic | 30.0% | |
| TTATCCTTGCCTTGTATTTA+TGG | + | chr8.4:6375592-6375611 | MS.gene068867:intron | 30.0% | |
| TTGCTTATAATTGACAACAG+AGG | - | chr8.4:6374640-6374659 | None:intergenic | 30.0% | |
| ! | ACATTGAGGGAAATTTTACT+GGG | + | chr8.4:6374243-6374262 | MS.gene068867:CDS | 30.0% |
| ! | CATTTGAGTTTCACAATAAC+AGG | - | chr8.4:6373886-6373905 | None:intergenic | 30.0% |
| ! | TACATTGAGGGAAATTTTAC+TGG | + | chr8.4:6374242-6374261 | MS.gene068867:CDS | 30.0% |
| !! | ACTTAGGAGAGATTTTAATC+AGG | + | chr8.4:6374267-6374286 | MS.gene068867:CDS | 30.0% |
| !! | CATTCTTTTTGCTACTTCTT+TGG | + | chr8.4:6374149-6374168 | MS.gene068867:intron | 30.0% |
| AAAAAAGGCTTGGGATTAAC+AGG | - | chr8.4:6373844-6373863 | None:intergenic | 35.0% | |
| AAAATGTCTGACTTTGGCAT+TGG | + | chr8.4:6375682-6375701 | MS.gene068867:intron | 35.0% | |
| AACGAAGACATAACTAGAAG+TGG | - | chr8.4:6375418-6375437 | None:intergenic | 35.0% | |
| AACTCCCCAAATAAACATCA+AGG | + | chr8.4:6375473-6375492 | MS.gene068867:intron | 35.0% | |
| AATGATGCATCAGATGCTAA+TGG | - | chr8.4:6375024-6375043 | None:intergenic | 35.0% | |
| ACACCCTTGATGTTTATTTG+GGG | - | chr8.4:6375480-6375499 | None:intergenic | 35.0% | |
| ACGAAGACATAACTAGAAGT+GGG | - | chr8.4:6375417-6375436 | None:intergenic | 35.0% | |
| ACTCCCCAAATAAACATCAA+GGG | + | chr8.4:6375474-6375493 | MS.gene068867:intron | 35.0% | |
| ATGGACAAAATGTCTGACTT+TGG | + | chr8.4:6375676-6375695 | MS.gene068867:intron | 35.0% | |
| ATGTTGAGAAATCATGTGCT+AGG | - | chr8.4:6374341-6374360 | None:intergenic | 35.0% | |
| ATTAAAAGAGGTCACACAGT+CGG | - | chr8.4:6375758-6375777 | None:intergenic | 35.0% | |
| CACACCCTTGATGTTTATTT+GGG | - | chr8.4:6375481-6375500 | None:intergenic | 35.0% | |
| CAGCTGCTAAAACAATACAA+AGG | + | chr8.4:6374802-6374821 | MS.gene068867:intron | 35.0% | |
| GCAAAAAGAATGGTCAAAAC+CGG | - | chr8.4:6374141-6374160 | None:intergenic | 35.0% | |
| GGTATCATTAACGGGATATT+GGG | - | chr8.4:6375003-6375022 | None:intergenic | 35.0% | |
| TAGCACATGATTTCTCAACA+TGG | + | chr8.4:6374340-6374359 | MS.gene068867:intron | 35.0% | |
| TAGTTATGTCTTCGTTAGCT+TGG | + | chr8.4:6375422-6375441 | MS.gene068867:intron | 35.0% | |
| TCACACCCTTGATGTTTATT+TGG | - | chr8.4:6375482-6375501 | None:intergenic | 35.0% | |
| TCACCGTTAAAGCAATTGAA+GGG | + | chr8.4:6375380-6375399 | MS.gene068867:intron | 35.0% | |
| TGCTTATAATTGACAACGGA+GGG | + | chr8.4:6374756-6374775 | MS.gene068867:intron | 35.0% | |
| TGGTATCATTAACGGGATAT+TGG | - | chr8.4:6375004-6375023 | None:intergenic | 35.0% | |
| TGTAGGAATCAACTGAAACA+AGG | - | chr8.4:6374080-6374099 | None:intergenic | 35.0% | |
| TGTTTATCCAAACATGCACT+AGG | - | chr8.4:6375077-6375096 | None:intergenic | 35.0% | |
| TTCAATTGCTTTAACGGTGA+AGG | - | chr8.4:6375380-6375399 | None:intergenic | 35.0% | |
| TTCACCGTTAAAGCAATTGA+AGG | + | chr8.4:6375379-6375398 | MS.gene068867:intron | 35.0% | |
| TTGCTTATAATTGACAACGG+AGG | + | chr8.4:6374755-6374774 | MS.gene068867:intron | 35.0% | |
| ! | AACACTGAAGAGTACATTGA+GGG | + | chr8.4:6374230-6374249 | MS.gene068867:CDS | 35.0% |
| ! | AATAAACATCAAGGGTGTGA+TGG | + | chr8.4:6375482-6375501 | MS.gene068867:intron | 35.0% |
| ! | GGAAATTTTACTGGGAACTT+AGG | + | chr8.4:6374251-6374270 | MS.gene068867:CDS | 35.0% |
| AAATGGGGAATGGAGTACAA+AGG | + | chr8.4:6373901-6373920 | MS.gene068867:CDS | 40.0% | |
| AACAATGTTCTCTACATGCG+CGG | + | chr8.4:6375837-6375856 | MS.gene068867:CDS | 40.0% | |
| AATTGCTTTAACGGTGAAGG+AGG | - | chr8.4:6375377-6375396 | None:intergenic | 40.0% | |
| ATAGCGCAGTCTCAATTTAC+AGG | - | chr8.4:6375053-6375072 | None:intergenic | 40.0% | |
| CACACCATAAATACAAGGCA+AGG | - | chr8.4:6375599-6375618 | None:intergenic | 40.0% | |
| CGAAGACATAACTAGAAGTG+GGG | - | chr8.4:6375416-6375435 | None:intergenic | 40.0% | |
| CTCAATGTACTCTTCAGTGT+TGG | - | chr8.4:6374231-6374250 | None:intergenic | 40.0% | |
| TCTTCGATTTCTTCATCCTC+TGG | - | chr8.4:6375867-6375886 | None:intergenic | 40.0% | |
| TGAGCTTATCTACTGACGTA+AGG | - | chr8.4:6375310-6375329 | None:intergenic | 40.0% | |
| TGATTCCTACATGAACTTGC+AGG | + | chr8.4:6374089-6374108 | MS.gene068867:CDS | 40.0% | |
| TGGCCCTTCAATTGCTTTAA+CGG | - | chr8.4:6375386-6375405 | None:intergenic | 40.0% | |
| TGTGAAACTCAAATGGGGAA+TGG | + | chr8.4:6373891-6373910 | MS.gene068867:CDS | 40.0% | |
| TTGTACCTGCAAGTTCATGT+AGG | - | chr8.4:6374097-6374116 | None:intergenic | 40.0% | |
| ! | CAACACTGAAGAGTACATTG+AGG | + | chr8.4:6374229-6374248 | MS.gene068867:CDS | 40.0% |
| !! | AGGAAATGCTTCTGAAGCAA+TGG | + | chr8.4:6374919-6374938 | MS.gene068867:intron | 40.0% |
| !! | GGAAATGCTTCTGAAGCAAT+GGG | + | chr8.4:6374920-6374939 | MS.gene068867:intron | 40.0% |
| !! | TGGTGTTGTATTTCTGAGCA+TGG | + | chr8.4:6375657-6375676 | MS.gene068867:intron | 40.0% |
| !!! | ACTAGAAGTGGGGTTTTGTT+TGG | - | chr8.4:6375406-6375425 | None:intergenic | 40.0% |
| !!! | TGGGATCTTTTAGTTCCATC+TGG | - | chr8.4:6374957-6374976 | None:intergenic | 40.0% |
| CGCTATACCTAGTGCATGTT+TGG | + | chr8.4:6375067-6375086 | MS.gene068867:intron | 45.0% | |
| CTTGTTGGGGTAATACGACT+TGG | - | chr8.4:6374848-6374867 | None:intergenic | 45.0% | |
| TAAAGCGACCTCGATCTAGT+TGG | + | chr8.4:6374521-6374540 | MS.gene068867:intron | 45.0% | |
| TCTGTGAACTTCTCTGTAGC+TGG | + | chr8.4:6374205-6374224 | MS.gene068867:intron | 45.0% | |
| TGGCTAAGCATGCTCTTGTT+GGG | - | chr8.4:6374862-6374881 | None:intergenic | 45.0% | |
| TTGGCTAAGCATGCTCTTGT+TGG | - | chr8.4:6374863-6374882 | None:intergenic | 45.0% | |
| ! | AGTTCCATCTGGTTTGAGCA+AGG | - | chr8.4:6374946-6374965 | None:intergenic | 45.0% |
| ! | CTAGATACACTAGTTGGTGC+CGG | + | chr8.4:6374119-6374138 | MS.gene068867:intron | 45.0% |
| AAGGAGGAAGCTCTCCTAGA+AGG | - | chr8.4:6375361-6375380 | None:intergenic | 50.0% | |
| AGGAGGAAGCTCTCCTAGAA+GGG | - | chr8.4:6375360-6375379 | None:intergenic | 50.0% | |
| GGCTAAGCATGCTCTTGTTG+GGG | - | chr8.4:6374861-6374880 | None:intergenic | 50.0% | |
| AATAAGCCGCTGCCCCTTCT+AGG | + | chr8.4:6375344-6375363 | MS.gene068867:intron | 55.0% | |
| AGAAGGGGCAGCGGCTTATT+TGG | - | chr8.4:6375344-6375363 | None:intergenic | 55.0% | |
| GGAGGAAGCTCTCCTAGAAG+GGG | - | chr8.4:6375359-6375378 | None:intergenic | 55.0% | |
| TGGGCCTTGCTCAAACCAGA+TGG | + | chr8.4:6374939-6374958 | MS.gene068867:intron | 55.0% | |
| AGCTCTCCTAGAAGGGGCAG+CGG | - | chr8.4:6375353-6375372 | None:intergenic | 60.0% | |
| ! | CTACATGCGCGGAGTACCAG+AGG | + | chr8.4:6375848-6375867 | MS.gene068867:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 6373835 | 6375902 | 6373835 | ID=MS.gene068867 |
| chr8.4 | mRNA | 6373835 | 6375902 | 6373835 | ID=MS.gene068867.t1;Parent=MS.gene068867 |
| chr8.4 | exon | 6373835 | 6373922 | 6373835 | ID=MS.gene068867.t1.exon1;Parent=MS.gene068867.t1 |
| chr8.4 | CDS | 6373835 | 6373922 | 6373835 | ID=cds.MS.gene068867.t1;Parent=MS.gene068867.t1 |
| chr8.4 | exon | 6374073 | 6374110 | 6374073 | ID=MS.gene068867.t1.exon2;Parent=MS.gene068867.t1 |
| chr8.4 | CDS | 6374073 | 6374110 | 6374073 | ID=cds.MS.gene068867.t1;Parent=MS.gene068867.t1 |
| chr8.4 | exon | 6374224 | 6374288 | 6374224 | ID=MS.gene068867.t1.exon3;Parent=MS.gene068867.t1 |
| chr8.4 | CDS | 6374224 | 6374288 | 6374224 | ID=cds.MS.gene068867.t1;Parent=MS.gene068867.t1 |
| chr8.4 | exon | 6375833 | 6375902 | 6375833 | ID=MS.gene068867.t1.exon4;Parent=MS.gene068867.t1 |
| chr8.4 | CDS | 6375833 | 6375902 | 6375833 | ID=cds.MS.gene068867.t1;Parent=MS.gene068867.t1 |
| Gene Sequence |
| Protein sequence |
