Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012386.t1 | XP_003610112.1 | 100 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 2.80E-43 | 184.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012386.t1 | Q9SUM2 | 87.5 | 88 | 11 | 0 | 1 | 88 | 1 | 88 | 9.4e-40 | 163.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012386.t1 | G7JVF0 | 100.0 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 2.0e-43 | 184.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012386.t1 | MTR_4g128050 | 100.000 | 88 | 0 | 0 | 1 | 88 | 1 | 88 | 5.02e-61 | 180 |
| MS.gene012386.t1 | MTR_4g080300 | 45.455 | 66 | 36 | 0 | 8 | 73 | 15 | 80 | 1.64e-17 | 70.5 |
| MS.gene012386.t1 | MTR_7g114670 | 68.750 | 48 | 15 | 0 | 2 | 49 | 3 | 50 | 5.34e-17 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene012386.t1 | AT4G30220 | 87.500 | 88 | 11 | 0 | 1 | 88 | 1 | 88 | 2.32e-53 | 161 |
| MS.gene012386.t1 | AT4G30220 | 85.227 | 88 | 13 | 0 | 1 | 88 | 9 | 96 | 4.60e-52 | 158 |
| MS.gene012386.t1 | AT2G14285 | 83.607 | 61 | 10 | 0 | 28 | 88 | 1 | 61 | 1.27e-32 | 108 |
| MS.gene012386.t1 | AT3G59810 | 44.928 | 69 | 38 | 0 | 8 | 76 | 15 | 83 | 1.32e-17 | 70.9 |
| MS.gene012386.t1 | AT3G59810 | 44.928 | 69 | 38 | 0 | 8 | 76 | 15 | 83 | 1.32e-17 | 70.9 |
| MS.gene012386.t1 | AT3G59810 | 44.928 | 69 | 38 | 0 | 8 | 76 | 15 | 83 | 1.32e-17 | 70.9 |
| MS.gene012386.t1 | AT2G43810 | 45.455 | 66 | 36 | 0 | 8 | 73 | 15 | 80 | 1.39e-17 | 70.9 |
| MS.gene012386.t1 | AT2G43810 | 45.455 | 66 | 36 | 0 | 8 | 73 | 15 | 80 | 1.39e-17 | 70.9 |
| MS.gene012386.t1 | AT2G43810 | 45.455 | 66 | 36 | 0 | 8 | 73 | 15 | 80 | 1.39e-17 | 70.9 |
Find 15 sgRNAs with CRISPR-Local
Find 116 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTGAGCTTCACAATAAC+AGG | 0.299146 | 8.3:-3102663 | None:intergenic |
| CTGTTATTGTGAAGCTCAAA+TGG | 0.366952 | 8.3:+3102664 | MS.gene012386:CDS |
| TGATTCCTACATGAACTTGC+AGG | 0.426591 | 8.3:+3102836 | MS.gene012386:CDS |
| TCTTCGATTTCTTCATCCTC+TGG | 0.426615 | 8.3:-3104610 | None:intergenic |
| TGTTATTGTGAAGCTCAAAT+GGG | 0.480168 | 8.3:+3102665 | MS.gene012386:CDS |
| CTCAATGTACTCTTCAGTGT+TGG | 0.496731 | 8.3:-3102975 | None:intergenic |
| TGTGAAGCTCAAATGGGGAA+TGG | 0.566406 | 8.3:+3102671 | MS.gene012386:CDS |
| AACACTGAAGAGTACATTGA+GGG | 0.587864 | 8.3:+3102977 | MS.gene012386:CDS |
| CTACATGCGCGGAGTACCAG+AGG | 0.623589 | 8.3:+3104594 | MS.gene012386:CDS |
| TTGTACCTGCAAGTTCATGT+AGG | 0.632824 | 8.3:-3102841 | None:intergenic |
| AACAATGTTCTCTACATGCG+CGG | 0.654078 | 8.3:+3104583 | MS.gene012386:CDS |
| TGTAGGAATCAACTGAAACA+AGG | 0.659877 | 8.3:-3102824 | None:intergenic |
| GTTATTGTGAAGCTCAAATG+GGG | 0.660645 | 8.3:+3102666 | MS.gene012386:CDS |
| AAATGGGGAATGGAGTACAA+AGG | 0.680083 | 8.3:+3102681 | MS.gene012386:CDS |
| CAACACTGAAGAGTACATTG+AGG | 0.691161 | 8.3:+3102976 | MS.gene012386:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATAATATTTTTTATTAAA+AGG | + | chr8.3:3104369-3104388 | MS.gene012386:intron | 0.0% |
| !! | AAATAAAATTAACACTGAAA+TGG | - | chr8.3:3102728-3102747 | None:intergenic | 15.0% |
| !! | AGAAATAACACTATTAAAAG+AGG | - | chr8.3:3104516-3104535 | None:intergenic | 20.0% |
| !! | ATTAAAAGGTCTGAATATAT+TGG | + | chr8.3:3104383-3104402 | MS.gene012386:intron | 20.0% |
| !! | ATTCAATTGTTTGTATTTGT+AGG | + | chr8.3:3104557-3104576 | MS.gene012386:intron | 20.0% |
| !! | CATTCAATTAATTATGAATG+TGG | + | chr8.3:3103420-3103439 | MS.gene012386:intron | 20.0% |
| !! | GAAATGGAACATAATTATAA+AGG | - | chr8.3:3102712-3102731 | None:intergenic | 20.0% |
| !!! | AATCTTAATATTTTTAGTGC+AGG | + | chr8.3:3104296-3104315 | MS.gene012386:intron | 20.0% |
| !!! | AATTTGCTTATAATTGACAA+CGG | + | chr8.3:3103494-3103513 | MS.gene012386:intron | 20.0% |
| !!! | AGTTTTGTTTATCTATCATT+TGG | + | chr8.3:3103153-3103172 | MS.gene012386:intron | 20.0% |
| !!! | ATATCTTTTAGTGATTATGT+GGG | - | chr8.3:3103718-3103737 | None:intergenic | 20.0% |
| !!! | TAAGCAAATTTTGACTTTTT+AGG | + | chr8.3:3103395-3103414 | MS.gene012386:intron | 20.0% |
| !!! | TTTTATCTTCATGAAAAGTT+TGG | + | chr8.3:3104476-3104495 | MS.gene012386:intron | 20.0% |
| ! | AAAGTATAGAAGATTCTGAT+CGG | - | chr8.3:3102479-3102498 | None:intergenic | 25.0% |
| ! | AAATTGTTCAAAAAAGGCTT+GGG | - | chr8.3:3102633-3102652 | None:intergenic | 25.0% |
| ! | AATCTTCTATACTTTCGATT+AGG | + | chr8.3:3102484-3102503 | MS.gene012386:intron | 25.0% |
| ! | ATCATATAGCATATATCTTC+TGG | - | chr8.3:3103211-3103230 | None:intergenic | 25.0% |
| ! | GCTAATATAAGCAAAAGAAT+TGG | - | chr8.3:3103316-3103335 | None:intergenic | 25.0% |
| ! | TAAGGTTTGTAAGATTGTTT+GGG | - | chr8.3:3104034-3104053 | None:intergenic | 25.0% |
| ! | TCAAATAAGTCAATCCAAAT+AGG | + | chr8.3:3104069-3104088 | MS.gene012386:intron | 25.0% |
| ! | TTTAAATTGATGTCGAAACT+GGG | + | chr8.3:3103125-3103144 | MS.gene012386:intron | 25.0% |
| !! | CATATCTTTTAGTGATTATG+TGG | - | chr8.3:3103719-3103738 | None:intergenic | 25.0% |
| !! | TTAGCTCTTCTAATATGATA+AGG | + | chr8.3:3103641-3103660 | MS.gene012386:intron | 25.0% |
| !! | TTTTAAATTGATGTCGAAAC+TGG | + | chr8.3:3103124-3103143 | MS.gene012386:intron | 25.0% |
| !!! | TCATCTATTTTATTTGCTAC+TGG | + | chr8.3:3103048-3103067 | MS.gene012386:intron | 25.0% |
| !!! | TTGTGTTGTTTTTATTTTGC+AGG | + | chr8.3:3102798-3102817 | MS.gene012386:intron | 25.0% |
| !!! | TTTTCTCTATGATTTGTGTA+CGG | + | chr8.3:3102537-3102556 | MS.gene012386:intron | 25.0% |
| AATGATGAATCAGATGCTAA+TGG | - | chr8.3:3103766-3103785 | None:intergenic | 30.0% | |
| ACAATTCTAGATACACTAGT+TGG | + | chr8.3:3102860-3102879 | MS.gene012386:intron | 30.0% | |
| ACCCTTGATGTTTGTTTATT+TGG | - | chr8.3:3104224-3104243 | None:intergenic | 30.0% | |
| ATGCTAATGGTATCATTAAC+GGG | - | chr8.3:3103753-3103772 | None:intergenic | 30.0% | |
| ATTATAAACCAACTAGATCG+AGG | - | chr8.3:3103274-3103293 | None:intergenic | 30.0% | |
| CAAAGAAGTAGCAAAAAGAA+TGG | - | chr8.3:3102898-3102917 | None:intergenic | 30.0% | |
| CAAATTGTTCAAAAAAGGCT+TGG | - | chr8.3:3102634-3102653 | None:intergenic | 30.0% | |
| CCAGTCAAATTGTTCAAAAA+AGG | - | chr8.3:3102639-3102658 | None:intergenic | 30.0% | |
| CCCAAATAAACAAACATCAA+GGG | + | chr8.3:3104220-3104239 | MS.gene012386:intron | 30.0% | |
| CCCTTGATGTTTGTTTATTT+GGG | - | chr8.3:3104223-3104242 | None:intergenic | 30.0% | |
| CCTTGATGTTTGTTTATTTG+GGG | - | chr8.3:3104222-3104241 | None:intergenic | 30.0% | |
| GAGAAAATTAGATCGCATTA+AGG | - | chr8.3:3102524-3102543 | None:intergenic | 30.0% | |
| GATGCTAATGGTATCATTAA+CGG | - | chr8.3:3103754-3103773 | None:intergenic | 30.0% | |
| GTAAGGTTTGTAAGATTGTT+TGG | - | chr8.3:3104035-3104054 | None:intergenic | 30.0% | |
| TAACAATGTAGCACATTTGT+TGG | - | chr8.3:3103624-3103643 | None:intergenic | 30.0% | |
| TACAACACACCATAAATACA+AGG | - | chr8.3:3104350-3104369 | None:intergenic | 30.0% | |
| TCAAATCAACAACAAACAAC+AGG | - | chr8.3:3103242-3103261 | None:intergenic | 30.0% | |
| TGCTTATAATTGACAACAGA+GGG | - | chr8.3:3103381-3103400 | None:intergenic | 30.0% | |
| TGTTATTGTGAAGCTCAAAT+GGG | + | chr8.3:3102665-3102684 | MS.gene012386:CDS | 30.0% | |
| TTATCCTTGCCTTGTATTTA+TGG | + | chr8.3:3104338-3104357 | MS.gene012386:intron | 30.0% | |
| TTGCTTATAATTGACAACAG+AGG | - | chr8.3:3103382-3103401 | None:intergenic | 30.0% | |
| ! | ACATTGAGGGAAATTTTACT+GGG | + | chr8.3:3102990-3103009 | MS.gene012386:CDS | 30.0% |
| ! | TACATTGAGGGAAATTTTAC+TGG | + | chr8.3:3102989-3103008 | MS.gene012386:CDS | 30.0% |
| !! | ACTTAGGAGAGATTTTAATC+AGG | + | chr8.3:3103014-3103033 | MS.gene012386:CDS | 30.0% |
| !! | CATTCTTTTTGCTACTTCTT+TGG | + | chr8.3:3102896-3102915 | MS.gene012386:intron | 30.0% |
| !!! | CCTTTTTTGAACAATTTGAC+TGG | + | chr8.3:3102636-3102655 | MS.gene012386:CDS | 30.0% |
| AAAAAAGGCTTGGGATTAAC+AGG | - | chr8.3:3102624-3102643 | None:intergenic | 35.0% | |
| AACGAAGACATAACTAGAAG+TGG | - | chr8.3:3104160-3104179 | None:intergenic | 35.0% | |
| ACGAAGACATAACTAGAAGT+GGG | - | chr8.3:3104159-3104178 | None:intergenic | 35.0% | |
| ATGTTGAGAAATCATGTGCT+AGG | - | chr8.3:3103088-3103107 | None:intergenic | 35.0% | |
| ATTAAAAGAGGTCACACAGT+CGG | - | chr8.3:3104504-3104523 | None:intergenic | 35.0% | |
| CAGCTGCTAAAACAATACAA+AGG | + | chr8.3:3103544-3103563 | MS.gene012386:intron | 35.0% | |
| CATTTGAGCTTCACAATAAC+AGG | - | chr8.3:3102666-3102685 | None:intergenic | 35.0% | |
| CCCCAAATAAACAAACATCA+AGG | + | chr8.3:3104219-3104238 | MS.gene012386:intron | 35.0% | |
| CTGTTATTGTGAAGCTCAAA+TGG | + | chr8.3:3102664-3102683 | MS.gene012386:CDS | 35.0% | |
| GCAAAAAGAATGGTCAAAAC+CGG | - | chr8.3:3102888-3102907 | None:intergenic | 35.0% | |
| GGTATCATTAACGGGATATT+GGG | - | chr8.3:3103745-3103764 | None:intergenic | 35.0% | |
| GTTATTGTGAAGCTCAAATG+GGG | + | chr8.3:3102666-3102685 | MS.gene012386:CDS | 35.0% | |
| TAGCACATGATTTCTCAACA+TGG | + | chr8.3:3103087-3103106 | MS.gene012386:intron | 35.0% | |
| TAGTTATGTCTTCGTTAGCT+TGG | + | chr8.3:3104164-3104183 | MS.gene012386:intron | 35.0% | |
| TATAGAAGATTCTGATCGGA+GGG | - | chr8.3:3102475-3102494 | None:intergenic | 35.0% | |
| TGCTTATAATTGACAACGGA+GGG | + | chr8.3:3103498-3103517 | MS.gene012386:intron | 35.0% | |
| TGGTATCATTAACGGGATAT+TGG | - | chr8.3:3103746-3103765 | None:intergenic | 35.0% | |
| TGGTCCTTCAATTGCTTTAA+CGG | - | chr8.3:3104128-3104147 | None:intergenic | 35.0% | |
| TGTAGGAATCAACTGAAACA+AGG | - | chr8.3:3102827-3102846 | None:intergenic | 35.0% | |
| TGTTTATCCAAACATGCACT+AGG | - | chr8.3:3103819-3103838 | None:intergenic | 35.0% | |
| TTCAATTGCTTTAACGGTGA+AGG | - | chr8.3:3104122-3104141 | None:intergenic | 35.0% | |
| TTCACCGTTAAAGCAATTGA+AGG | + | chr8.3:3104121-3104140 | MS.gene012386:intron | 35.0% | |
| TTGCTTATAATTGACAACGG+AGG | + | chr8.3:3103497-3103516 | MS.gene012386:intron | 35.0% | |
| ! | AACACTGAAGAGTACATTGA+GGG | + | chr8.3:3102977-3102996 | MS.gene012386:CDS | 35.0% |
| ! | GGAAATTTTACTGGGAACTT+AGG | + | chr8.3:3102998-3103017 | MS.gene012386:CDS | 35.0% |
| AAATGGGGAATGGAGTACAA+AGG | + | chr8.3:3102681-3102700 | MS.gene012386:CDS | 40.0% | |
| AACAATGTTCTCTACATGCG+CGG | + | chr8.3:3104583-3104602 | MS.gene012386:CDS | 40.0% | |
| ATAGCGCAGTCTCAATTTAC+AGG | - | chr8.3:3103795-3103814 | None:intergenic | 40.0% | |
| CACACCATAAATACAAGGCA+AGG | - | chr8.3:3104345-3104364 | None:intergenic | 40.0% | |
| CGAAGACATAACTAGAAGTG+GGG | - | chr8.3:3104158-3104177 | None:intergenic | 40.0% | |
| CGGAGGGAAAAAGATACTTA+CGG | - | chr8.3:3102459-3102478 | None:intergenic | 40.0% | |
| CTCAATGTACTCTTCAGTGT+TGG | - | chr8.3:3102978-3102997 | None:intergenic | 40.0% | |
| GTATAGAAGATTCTGATCGG+AGG | - | chr8.3:3102476-3102495 | None:intergenic | 40.0% | |
| TCTTCGATTTCTTCATCCTC+TGG | - | chr8.3:3104613-3104632 | None:intergenic | 40.0% | |
| TGAGCTTATCTACTGACGTA+AGG | - | chr8.3:3104052-3104071 | None:intergenic | 40.0% | |
| TGATTCCTACATGAACTTGC+AGG | + | chr8.3:3102836-3102855 | MS.gene012386:CDS | 40.0% | |
| TTGTACCTGCAAGTTCATGT+AGG | - | chr8.3:3102844-3102863 | None:intergenic | 40.0% | |
| ! | AACAAACATCAAGGGTGTGA+TGG | + | chr8.3:3104228-3104247 | MS.gene012386:intron | 40.0% |
| ! | ATGGACAAAATGTCTGGCTT+TGG | + | chr8.3:3104422-3104441 | MS.gene012386:intron | 40.0% |
| ! | CAACACTGAAGAGTACATTG+AGG | + | chr8.3:3102976-3102995 | MS.gene012386:CDS | 40.0% |
| !! | AAAATGTCTGGCTTTGGCAT+TGG | + | chr8.3:3104428-3104447 | MS.gene012386:intron | 40.0% |
| !! | AGGAAATGCTTCTGAAGCAA+TGG | + | chr8.3:3103661-3103680 | MS.gene012386:intron | 40.0% |
| !! | GGAAATGCTTCTGAAGCAAT+GGG | + | chr8.3:3103662-3103681 | MS.gene012386:intron | 40.0% |
| !! | TGGTGTTGTATTTCTGAGCA+TGG | + | chr8.3:3104403-3104422 | MS.gene012386:intron | 40.0% |
| !!! | ACTAGAAGTGGGGTTTTGTT+TGG | - | chr8.3:3104148-3104167 | None:intergenic | 40.0% |
| !!! | TGGGATCTTTTAGTTCCATC+TGG | - | chr8.3:3103699-3103718 | None:intergenic | 40.0% |
| AAGGAGAAAGCTCTCCTAGA+AGG | - | chr8.3:3104103-3104122 | None:intergenic | 45.0% | |
| CGCTATACCTAGTGCATGTT+TGG | + | chr8.3:3103809-3103828 | MS.gene012386:intron | 45.0% | |
| CTGAGCATGGACAAAATGTC+TGG | + | chr8.3:3104416-3104435 | MS.gene012386:intron | 45.0% | |
| CTTGTTGGGGTAATACGACT+TGG | - | chr8.3:3103590-3103609 | None:intergenic | 45.0% | |
| TAAAGCGACCTCGATCTAGT+TGG | + | chr8.3:3103263-3103282 | MS.gene012386:intron | 45.0% | |
| TCTGTGAACTTCTCTGTAGC+TGG | + | chr8.3:3102952-3102971 | MS.gene012386:intron | 45.0% | |
| TGGCTAAGCATGCTCTTGTT+GGG | - | chr8.3:3103604-3103623 | None:intergenic | 45.0% | |
| TGTGAAGCTCAAATGGGGAA+TGG | + | chr8.3:3102671-3102690 | MS.gene012386:CDS | 45.0% | |
| TTGGCTAAGCATGCTCTTGT+TGG | - | chr8.3:3103605-3103624 | None:intergenic | 45.0% | |
| ! | AGTTCCATCTGGTTTGAGCA+AGG | - | chr8.3:3103688-3103707 | None:intergenic | 45.0% |
| ! | CTAGATACACTAGTTGGTGC+CGG | + | chr8.3:3102866-3102885 | MS.gene012386:intron | 45.0% |
| GGCTAAGCATGCTCTTGTTG+GGG | - | chr8.3:3103603-3103622 | None:intergenic | 50.0% | |
| AATAGGCCGCTGCTCCTTCT+AGG | + | chr8.3:3104086-3104105 | MS.gene012386:intron | 55.0% | |
| AGAAGGAGCAGCGGCCTATT+TGG | - | chr8.3:3104086-3104105 | None:intergenic | 55.0% | |
| AGCTCTCCTAGAAGGAGCAG+CGG | - | chr8.3:3104095-3104114 | None:intergenic | 55.0% | |
| TGGGCCTTGCTCAAACCAGA+TGG | + | chr8.3:3103681-3103700 | MS.gene012386:intron | 55.0% | |
| ! | CTACATGCGCGGAGTACCAG+AGG | + | chr8.3:3104594-3104613 | MS.gene012386:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 3102452 | 3104648 | 3102452 | ID=MS.gene012386 |
| chr8.3 | mRNA | 3102452 | 3104648 | 3102452 | ID=MS.gene012386.t1;Parent=MS.gene012386 |
| chr8.3 | exon | 3102452 | 3102457 | 3102452 | ID=MS.gene012386.t1.exon1;Parent=MS.gene012386.t1 |
| chr8.3 | CDS | 3102452 | 3102457 | 3102452 | ID=cds.MS.gene012386.t1;Parent=MS.gene012386.t1 |
| chr8.3 | exon | 3102615 | 3102702 | 3102615 | ID=MS.gene012386.t1.exon2;Parent=MS.gene012386.t1 |
| chr8.3 | CDS | 3102615 | 3102702 | 3102615 | ID=cds.MS.gene012386.t1;Parent=MS.gene012386.t1 |
| chr8.3 | exon | 3102820 | 3102857 | 3102820 | ID=MS.gene012386.t1.exon3;Parent=MS.gene012386.t1 |
| chr8.3 | CDS | 3102820 | 3102857 | 3102820 | ID=cds.MS.gene012386.t1;Parent=MS.gene012386.t1 |
| chr8.3 | exon | 3102971 | 3103035 | 3102971 | ID=MS.gene012386.t1.exon4;Parent=MS.gene012386.t1 |
| chr8.3 | CDS | 3102971 | 3103035 | 3102971 | ID=cds.MS.gene012386.t1;Parent=MS.gene012386.t1 |
| chr8.3 | exon | 3104579 | 3104648 | 3104579 | ID=MS.gene012386.t1.exon5;Parent=MS.gene012386.t1 |
| chr8.3 | CDS | 3104579 | 3104648 | 3104579 | ID=cds.MS.gene012386.t1;Parent=MS.gene012386.t1 |
| Gene Sequence |
| Protein sequence |
