AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene020509


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020509.t1 MTR_8g105110 100.000 154 0 0 1 154 1 154 4.92e-111 312
MS.gene020509.t1 MTR_1g070450 95.455 154 5 1 1 154 1 152 4.61e-104 294
MS.gene020509.t1 MTR_4g075450 92.857 154 9 1 1 154 1 152 4.90e-92 264
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene020509.t1 AT5G09500 87.662 154 15 1 1 154 1 150 1.07e-93 268
MS.gene020509.t1 AT5G09510 91.558 154 11 1 1 154 1 152 3.57e-92 264
MS.gene020509.t1 AT1G04270 91.558 154 11 1 1 154 1 152 4.12e-92 264
MS.gene020509.t1 AT1G04270 90.909 154 11 2 1 154 1 151 1.39e-89 258
MS.gene020509.t1 AT5G09490 81.818 154 26 1 1 154 1 152 9.71e-89 255
MS.gene020509.t1 AT5G43640 85.906 149 21 0 6 154 1 149 1.10e-82 240
MS.gene020509.t1 AT5G09510 94.915 118 6 0 37 154 1 118 2.08e-71 210
MS.gene020509.t1 AT5G63070 62.500 160 54 2 1 154 1 160 3.08e-64 194
MS.gene020509.t1 AT1G33850 83.607 61 10 0 12 72 7 67 1.12e-19 78.2

Find 43 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATTTCAACTTGGTTAA+AGG 0.236449 4.3:-1455179 None:intergenic
GAGATGATTGGGAGCATTAT+TGG 0.247005 4.3:+1455139 MS.gene020509:CDS
CAGTTTCAGAGGAGTTGATT+TGG 0.292075 4.3:+1453905 MS.gene020509:CDS
CCAGGTCTACCATGCTTAAC+AGG 0.341486 4.3:-1455250 None:intergenic
TCAGTGCTCGTGCTCGTAGA+AGG 0.367007 4.3:+1453970 MS.gene020509:CDS
TTCTTGAATGTTCTCTTCTT+CGG 0.377124 4.3:-1453879 None:intergenic
AGATGGGTCCTAACAGGTTC+GGG 0.405557 4.3:-1455094 None:intergenic
GTGAGAAGCCCGAACCTGTT+AGG 0.409377 4.3:+1455086 MS.gene020509:CDS
GATGCAGCAGCCGCCGCCGC+GGG 0.422266 4.3:+1453852 MS.gene020509:CDS
TTACGAAGATGGGTCCTAAC+AGG 0.435557 4.3:-1455100 None:intergenic
AAGATGGGTCCTAACAGGTT+CGG 0.450751 4.3:-1455095 None:intergenic
CAATGATCATGTTACGAAGA+TGG 0.465315 4.3:-1455111 None:intergenic
ATCATTGTACCTGAGATGAT+TGG 0.468347 4.3:+1455127 MS.gene020509:CDS
TTCAAGAAGTTCAGTTTCAG+AGG 0.481387 4.3:+1453894 MS.gene020509:CDS
AGGTAATGACCGATCATCTC+AGG 0.492365 4.3:-1455202 None:intergenic
CCTGTTAAGCATGGTAGACC+TGG 0.509940 4.3:+1455250 MS.gene020509:CDS
AATGATCATGTTACGAAGAT+GGG 0.516658 4.3:-1455110 None:intergenic
GCATTATTGGAGTGTACAAT+GGG 0.517140 4.3:+1455152 MS.gene020509:CDS
ACAGGTTCGGGCTTCTCACC+AGG 0.517899 4.3:-1455082 None:intergenic
CTTCGGTACTCCCGCGGCGG+CGG 0.518043 4.3:-1453862 None:intergenic
TTGGTGCTACTCACTCCTCC+AGG 0.521787 4.3:+1455275 MS.gene020509:CDS
ATAATGCTCCCAATCATCTC+AGG 0.528225 4.3:-1455136 None:intergenic
TCATTGTACCTGAGATGATT+GGG 0.539509 4.3:+1455128 MS.gene020509:CDS
GCTTACGAGTGAGACCTCTC+TGG 0.541580 4.3:-1453995 None:intergenic
TCTCAGGTTTGATTTCAACT+TGG 0.546765 4.3:-1455186 None:intergenic
AGCATTATTGGAGTGTACAA+TGG 0.558872 4.3:+1455151 MS.gene020509:CDS
TAGAAAAGGGAGGCACCACC+TGG 0.561975 4.3:+1455064 MS.gene020509:intron
TCACTTGAGAGGAATGAACC+TGG 0.566509 4.3:-1455293 None:intergenic
AGATGCAGCAGCCGCCGCCG+CGG 0.579389 4.3:+1453851 MS.gene020509:CDS
GAGTGAGTAGCACCAATACC+AGG 0.584094 4.3:-1455268 None:intergenic
GAAATCAAACCTGAGATGAT+CGG 0.585387 4.3:+1455193 MS.gene020509:CDS
AAGCATGGTAGACCTGGTAT+TGG 0.586812 4.3:+1455256 MS.gene020509:CDS
TCATACAAGCCTGTTAAGCA+TGG 0.604339 4.3:+1455241 MS.gene020509:CDS
GAGCTTAACAAGCTCATCAG+TGG 0.607114 4.3:-1453946 None:intergenic
AGGTCTCACTCGTAAGCCCA+TGG 0.623580 4.3:+1454001 MS.gene020509:CDS
AACAAGGTGATTCACTTGAG+AGG 0.631770 4.3:-1455304 None:intergenic
ATGAGATCGAGAATTCAGCT+AGG 0.682732 4.3:-1455222 None:intergenic
GGTTCGGGCTTCTCACCAGG+TGG 0.683492 4.3:-1455079 None:intergenic
TCTCTTCTTCGGTACTCCCG+CGG 0.697626 4.3:-1453868 None:intergenic
CTTCTTCGGTACTCCCGCGG+CGG 0.700206 4.3:-1453865 None:intergenic
GAATTGGTTGTAGAAAAGGG+AGG 0.733330 4.3:+1455054 MS.gene020509:intron
CTTGAGAGGAATGAACCTGG+AGG 0.737810 4.3:-1455290 None:intergenic
GCTCGTAGAAGGTTCCAGAG+AGG 0.763484 4.3:+1453981 MS.gene020509:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTACTATTTTACAAATTTA+AGG - chr4.3:1454187-1454206 None:intergenic 10.0%
!! ATTACTATTATGCAAATTTA+AGG - chr4.3:1454502-1454521 None:intergenic 15.0%
!!! AAAAAAAAGTGTTCAATTTA+AGG - chr4.3:1454531-1454550 None:intergenic 15.0%
!!! AAAAAAAGTGTTCAATTTAA+GGG - chr4.3:1454530-1454549 None:intergenic 15.0%
!!! ATTGTTATAATTTTGTGAAT+TGG + chr4.3:1455038-1455057 MS.gene020509:intron 15.0%
!!! GAACACTTTTTTTTTTTTTT+TGG + chr4.3:1454537-1454556 MS.gene020509:intron 15.0%
!!! TTTGTTATTCTTTTATTTAG+TGG + chr4.3:1454674-1454693 MS.gene020509:intron 15.0%
!!! TTTGTTATTTTGTTAATTTG+AGG + chr4.3:1454436-1454455 MS.gene020509:intron 15.0%
!! AAGGAACTAAATTGATATAT+TGG - chr4.3:1454483-1454502 None:intergenic 20.0%
!! ATATGCAAAATACTAACAAT+TGG + chr4.3:1454158-1454177 MS.gene020509:intron 20.0%
!! ATTCCAAATTCAAATTCAAT+CGG - chr4.3:1453805-1453824 None:intergenic 20.0%
!! TATGCAAAATACTAACAATT+GGG + chr4.3:1454159-1454178 MS.gene020509:intron 20.0%
!! TTTAGTACTTAAGATAGTAA+GGG - chr4.3:1455006-1455025 None:intergenic 20.0%
!!! GAAGTTTTTATTGATTTTGT+TGG + chr4.3:1454610-1454629 MS.gene020509:intron 20.0%
!!! GTTAGTATTTTGCATATTAA+AGG - chr4.3:1454155-1454174 None:intergenic 20.0%
!!! TGGTGGTTTTTTAAATTTAA+AGG + chr4.3:1454937-1454956 MS.gene020509:intron 20.0%
!!! TTGTTAATTTGTGAATTTGA+GGG + chr4.3:1454966-1454985 MS.gene020509:intron 20.0%
!!! TTTGTTAATTTGTGAATTTG+AGG + chr4.3:1454965-1454984 MS.gene020509:intron 20.0%
!!! TTTTAGTACTTAAGATAGTA+AGG - chr4.3:1455007-1455026 None:intergenic 20.0%
! AAATCCATGTTAGATTCTAT+CGG - chr4.3:1453782-1453801 None:intergenic 25.0%
! AACATGAAACCATTAAAATG+GGG - chr4.3:1454278-1454297 None:intergenic 25.0%
! AATAGCGATATTTAGGTATA+GGG + chr4.3:1454903-1454922 MS.gene020509:intron 25.0%
! AGTAATTGCATACCAAAAAT+TGG + chr4.3:1454201-1454220 MS.gene020509:intron 25.0%
! ATTTAACACTACCATTCTAA+AGG + chr4.3:1454801-1454820 MS.gene020509:intron 25.0%
! GAACATGAAACCATTAAAAT+GGG - chr4.3:1454279-1454298 None:intergenic 25.0%
! TGAACATGAAACCATTAAAA+TGG - chr4.3:1454280-1454299 None:intergenic 25.0%
! TTTCCGATTGAATTTGAATT+TGG + chr4.3:1453799-1453818 MS.gene020509:intron 25.0%
!! AAACAACAATCTTAGCATTT+TGG + chr4.3:1454112-1454131 MS.gene020509:intron 25.0%
!! AACAACAATCTTAGCATTTT+GGG + chr4.3:1454113-1454132 MS.gene020509:intron 25.0%
!! AGGACTAACATCAAAATTTT+TGG - chr4.3:1454712-1454731 None:intergenic 25.0%
!! ATATGACATTGTCCAATTTT+TGG - chr4.3:1454216-1454235 None:intergenic 25.0%
!! CTGAAGATATATTTTCATTG+AGG + chr4.3:1454382-1454401 MS.gene020509:intron 25.0%
!! GTGCTAATTTGCAAATATAA+AGG - chr4.3:1454732-1454751 None:intergenic 25.0%
!! TTTGATTTCAACTTGGTTAA+AGG - chr4.3:1455182-1455201 None:intergenic 25.0%
!!! AATTTGGAATTGAATTTTGC+AGG + chr4.3:1453815-1453834 MS.gene020509:intron 25.0%
!!! CTCTTGTTGATTATTGATTT+TGG + chr4.3:1454349-1454368 MS.gene020509:intron 25.0%
!!! GAAATTGATTGCTGTTTTTA+GGG + chr4.3:1454408-1454427 MS.gene020509:intron 25.0%
!!! TGTTGATTATTGATTTTGGT+GGG + chr4.3:1454353-1454372 MS.gene020509:intron 25.0%
!!! TTGTTGATTATTGATTTTGG+TGG + chr4.3:1454352-1454371 MS.gene020509:intron 25.0%
AAGGCATATAACCTTCAAAA+TGG - chr4.3:1454576-1454595 None:intergenic 30.0%
AATGATCATGTTACGAAGAT+GGG - chr4.3:1455113-1455132 None:intergenic 30.0%
ACTTCCATGTAAACTTGAAA+AGG - chr4.3:1454595-1454614 None:intergenic 30.0%
ATTTCCGATAGAATCTAACA+TGG + chr4.3:1453775-1453794 MS.gene020509:intron 30.0%
CTTACTGAATAGCGATATTT+AGG + chr4.3:1454896-1454915 MS.gene020509:intron 30.0%
GAATAGCGATATTTAGGTAT+AGG + chr4.3:1454902-1454921 MS.gene020509:intron 30.0%
TGTGAATTGGTTGTAGAAAA+GGG + chr4.3:1455051-1455070 MS.gene020509:intron 30.0%
TTCTTGAATGTTCTCTTCTT+CGG - chr4.3:1453882-1453901 None:intergenic 30.0%
TTGTGAATTGGTTGTAGAAA+AGG + chr4.3:1455050-1455069 MS.gene020509:intron 30.0%
! ACTTTAGTACCCCATTTTAA+TGG + chr4.3:1454266-1454285 MS.gene020509:intron 30.0%
! TTAAAATGGGGTACTAAAGT+CGG - chr4.3:1454266-1454285 None:intergenic 30.0%
!!! CGAAATTGATTGCTGTTTTT+AGG + chr4.3:1454407-1454426 MS.gene020509:intron 30.0%
!!! TATGCCTTTTCAAGTTTACA+TGG + chr4.3:1454588-1454607 MS.gene020509:intron 30.0%
AGCATTATTGGAGTGTACAA+TGG + chr4.3:1455151-1455170 MS.gene020509:CDS 35.0%
ATAGGGACTAAATTGATTGG+TGG + chr4.3:1454920-1454939 MS.gene020509:intron 35.0%
ATCATTGTACCTGAGATGAT+TGG + chr4.3:1455127-1455146 MS.gene020509:CDS 35.0%
ATCGGAAATCAAGTTTCAGA+AGG - chr4.3:1453764-1453783 None:intergenic 35.0%
CAATGATCATGTTACGAAGA+TGG - chr4.3:1455114-1455133 None:intergenic 35.0%
GAAATCAAACCTGAGATGAT+CGG + chr4.3:1455193-1455212 MS.gene020509:CDS 35.0%
GCATATAACCTTCAAAATGG+TGG - chr4.3:1454573-1454592 None:intergenic 35.0%
GCATTATTGGAGTGTACAAT+GGG + chr4.3:1455152-1455171 MS.gene020509:CDS 35.0%
GGTATAGGGACTAAATTGAT+TGG + chr4.3:1454917-1454936 MS.gene020509:intron 35.0%
GTGACTCAGTGTTCAAATTA+AGG - chr4.3:1454762-1454781 None:intergenic 35.0%
TCATTGTACCTGAGATGATT+GGG + chr4.3:1455128-1455147 MS.gene020509:CDS 35.0%
TTCAAGAAGTTCAGTTTCAG+AGG + chr4.3:1453894-1453913 MS.gene020509:CDS 35.0%
! ACGCATGTAAACCTTTAGAA+TGG - chr4.3:1454815-1454834 None:intergenic 35.0%
! CAAAGTTACCACCATTTTGA+AGG + chr4.3:1454562-1454581 MS.gene020509:intron 35.0%
! TCTCAGGTTTGATTTCAACT+TGG - chr4.3:1455189-1455208 None:intergenic 35.0%
ATAATGCTCCCAATCATCTC+AGG - chr4.3:1455139-1455158 None:intergenic 40.0%
ATGAGATCGAGAATTCAGCT+AGG - chr4.3:1455225-1455244 None:intergenic 40.0%
CATCAAAAAGCTTCGCAAAG+CGG + chr4.3:1454028-1454047 MS.gene020509:CDS 40.0%
GAATTGGTTGTAGAAAAGGG+AGG + chr4.3:1455054-1455073 MS.gene020509:intron 40.0%
TCATACAAGCCTGTTAAGCA+TGG + chr4.3:1455241-1455260 MS.gene020509:CDS 40.0%
! AAGCTTTTTGATGAGAGCCA+TGG - chr4.3:1454021-1454040 None:intergenic 40.0%
! AGCTTTTTGATGAGAGCCAT+GGG - chr4.3:1454020-1454039 None:intergenic 40.0%
! CAGTTTCAGAGGAGTTGATT+TGG + chr4.3:1453905-1453924 MS.gene020509:CDS 40.0%
!! GAGATGATTGGGAGCATTAT+TGG + chr4.3:1455139-1455158 MS.gene020509:CDS 40.0%
AAGATGGGTCCTAACAGGTT+CGG - chr4.3:1455098-1455117 None:intergenic 45.0%
AAGCATGGTAGACCTGGTAT+TGG + chr4.3:1455256-1455275 MS.gene020509:CDS 45.0%
AGGTAATGACCGATCATCTC+AGG - chr4.3:1455205-1455224 None:intergenic 45.0%
GAGCTTAACAAGCTCATCAG+TGG - chr4.3:1453949-1453968 None:intergenic 45.0%
TCACTTGAGAGGAATGAACC+TGG - chr4.3:1455296-1455315 None:intergenic 45.0%
TTACGAAGATGGGTCCTAAC+AGG - chr4.3:1455103-1455122 None:intergenic 45.0%
! AATTTTGCAGGCAGACGTTG+AGG + chr4.3:1453827-1453846 MS.gene020509:intron 45.0%
!! ATAAATAAAAGAAATAAAAA+TGG - chr4.3:1454069-1454088 None:intergenic 5.0%
!! TAAATAAAAGAAATAAAAAT+GGG - chr4.3:1454068-1454087 None:intergenic 5.0%
AGATGGGTCCTAACAGGTTC+GGG - chr4.3:1455097-1455116 None:intergenic 50.0%
CCAGGTCTACCATGCTTAAC+AGG - chr4.3:1455253-1455272 None:intergenic 50.0%
CCTGTTAAGCATGGTAGACC+TGG + chr4.3:1455250-1455269 MS.gene020509:CDS 50.0%
CTTGAGAGGAATGAACCTGG+AGG - chr4.3:1455293-1455312 None:intergenic 50.0%
GAGTGAGTAGCACCAATACC+AGG - chr4.3:1455271-1455290 None:intergenic 50.0%
AGGTCTCACTCGTAAGCCCA+TGG + chr4.3:1454001-1454020 MS.gene020509:CDS 55.0%
GCTCGTAGAAGGTTCCAGAG+AGG + chr4.3:1453981-1454000 MS.gene020509:CDS 55.0%
GCTTACGAGTGAGACCTCTC+TGG - chr4.3:1453998-1454017 None:intergenic 55.0%
GTGAGAAGCCCGAACCTGTT+AGG + chr4.3:1455086-1455105 MS.gene020509:CDS 55.0%
TCAGTGCTCGTGCTCGTAGA+AGG + chr4.3:1453970-1453989 MS.gene020509:CDS 55.0%
TCTCTTCTTCGGTACTCCCG+CGG - chr4.3:1453871-1453890 None:intergenic 55.0%
!! TAGAAAAGGGAGGCACCACC+TGG + chr4.3:1455064-1455083 MS.gene020509:intron 55.0%
!! TTGGTGCTACTCACTCCTCC+AGG + chr4.3:1455275-1455294 MS.gene020509:CDS 55.0%
ACAGGTTCGGGCTTCTCACC+AGG - chr4.3:1455085-1455104 None:intergenic 60.0%
CTTCTTCGGTACTCCCGCGG+CGG - chr4.3:1453868-1453887 None:intergenic 65.0%
GGTTCGGGCTTCTCACCAGG+TGG - chr4.3:1455082-1455101 None:intergenic 65.0%
AGATGCAGCAGCCGCCGCCG+CGG + chr4.3:1453851-1453870 MS.gene020509:CDS 75.0%
CTTCGGTACTCCCGCGGCGG+CGG - chr4.3:1453865-1453884 None:intergenic 75.0%
! GATGCAGCAGCCGCCGCCGC+GGG + chr4.3:1453852-1453871 MS.gene020509:CDS 80.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 1453745 1455315 1453745 ID=MS.gene020509
chr4.3 mRNA 1453745 1455315 1453745 ID=MS.gene020509.t1;Parent=MS.gene020509
chr4.3 exon 1453745 1453747 1453745 ID=MS.gene020509.t1.exon1;Parent=MS.gene020509.t1
chr4.3 CDS 1453745 1453747 1453745 ID=cds.MS.gene020509.t1;Parent=MS.gene020509.t1
chr4.3 exon 1453837 1454049 1453837 ID=MS.gene020509.t1.exon2;Parent=MS.gene020509.t1
chr4.3 CDS 1453837 1454049 1453837 ID=cds.MS.gene020509.t1;Parent=MS.gene020509.t1
chr4.3 exon 1455067 1455315 1455067 ID=MS.gene020509.t1.exon3;Parent=MS.gene020509.t1
chr4.3 CDS 1455067 1455315 1455067 ID=cds.MS.gene020509.t1;Parent=MS.gene020509.t1
Gene Sequence

>MS.gene020509

ATGGTGAGTTAACAATCCTTCTGAAACTTGATTTCCGATAGAATCTAACATGGATTTCCGATTGAATTTGAATTTGGAATTGAATTTTGCAGGCAGACGTTGAGGTAGATGCAGCAGCCGCCGCCGCGGGAGTACCGAAGAAGAGAACATTCAAGAAGTTCAGTTTCAGAGGAGTTGATTTGGATGCACTTCTTGACATGTCCACTGATGAGCTTGTTAAGCTCTTCAGTGCTCGTGCTCGTAGAAGGTTCCAGAGAGGTCTCACTCGTAAGCCCATGGCTCTCATCAAAAAGCTTCGCAAAGCGGTACAATTTTATTTTCCCATTTTTATTTCTTTTATTTATCTGAAAATTAAATAGTAAAATTAAAACAACAATCTTAGCATTTTGGGCGTGCTCAATTTAGTTCCTTTAATATGCAAAATACTAACAATTGGGTTCCTTAAATTTGTAAAATAGTAATTGCATACCAAAAATTGGACAATGTCATATCAGTCATTAGTATTTTTGTGTTTTTGTCCGACTTTAGTACCCCATTTTAATGGTTTCATGTTCATTTTGATGCAATAATTGCTTCTAGAACATATTCAAGTTACTATTCAAGTCTCTTGTTGATTATTGATTTTGGTGGGTTTGTTCTGAAGATATATTTTCATTGAGGTGCGAAATTGATTGCTGTTTTTAGGGATTAATTTGTTATTTTGTTAATTTGAGGCATTAAATTGCTCAAACTTTACCAATATATCAATTTAGTTCCTTAAATTTGCATAATAGTAATTTAGTCCCTTAAATTGAACACTTTTTTTTTTTTTTTGGTGCAAAGTTACCACCATTTTGAAGGTTATATGCCTTTTCAAGTTTACATGGAAGTTTTTATTGATTTTGTTGGATCTTTACTAAAGTTATATTTTGCTTGAAGAACTTAGTTATTTTGTTATTCTTTTATTTAGTGGTTAATTGAATTTCCAAAAATTTTGATGTTAGTCCTTTATATTTGCAAATTAGCACTTTAGTACCTTAATTTGAACACTGAGTCACTTTTTTTTTTTTGCTTTAAATTTAACACTACCATTCTAAAGGTTTACATGCGTCAAATGCATCAAGAGACTAGTTAAGTTAATATTTAAACAATTTTATTGATTTTGATGAATGCTTACTGAATAGCGATATTTAGGTATAGGGACTAAATTGATTGGTGGTTTTTTAAATTTAAAGGACTACTTTGTTAATTTGTGAATTTGAGGGACTAAATTACTTGACCCTTACTATCTTAAGTACTAAAATGCTGATTCATATTGTTATAATTTTGTGAATTGGTTGTAGAAAAGGGAGGCACCACCTGGTGAGAAGCCCGAACCTGTTAGGACCCATCTTCGTAACATGATCATTGTACCTGAGATGATTGGGAGCATTATTGGAGTGTACAATGGGAAGACCTTTAACCAAGTTGAAATCAAACCTGAGATGATCGGTCATTACCTAGCTGAATTCTCGATCTCATACAAGCCTGTTAAGCATGGTAGACCTGGTATTGGTGCTACTCACTCCTCCAGGTTCATTCCTCTCAAGTGA

Protein sequence

>MS.gene020509.t1

MADVEVDAAAAAAGVPKKRTFKKFSFRGVDLDALLDMSTDELVKLFSARARRRFQRGLTRKPMALIKKLRKAKREAPPGEKPEPVRTHLRNMIIVPEMIGSIIGVYNGKTFNQVEIKPEMIGHYLAEFSISYKPVKHGRPGIGATHSSRFIPLK