AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60717


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60717.t1 MTR_1g098170 100.000 152 0 0 1 152 1 152 6.47e-112 314
MS.gene60717.t1 MTR_1g098220 100.000 152 0 0 1 152 1 152 6.47e-112 314
MS.gene60717.t1 MTR_6g043210 98.684 152 2 0 1 152 1 152 6.70e-111 311
MS.gene60717.t1 MTR_1g492710 97.945 146 3 0 1 146 794 939 1.21e-97 303
MS.gene60717.t1 MTR_1g098220 99.160 119 1 0 1 119 1 119 2.71e-84 243
MS.gene60717.t1 MTR_5g027650 95.098 102 5 0 39 140 11 112 7.93e-69 203
MS.gene60717.t1 MTR_5g027580 93.137 102 7 0 39 140 48 149 7.08e-68 202
MS.gene60717.t1 MTR_5g027670 94.382 89 5 0 52 140 1 89 2.79e-58 176
MS.gene60717.t1 MTR_5g027615 94.382 89 5 0 52 140 1 89 7.00e-58 175
MS.gene60717.t1 MTR_5g027605 94.382 89 5 0 52 140 1 89 7.00e-58 175
MS.gene60717.t1 MTR_5g027640 94.382 89 5 0 52 140 1 89 7.00e-58 175
MS.gene60717.t1 MTR_5g027600 94.382 89 5 0 52 140 1 89 7.00e-58 175
MS.gene60717.t1 MTR_1005s0010 94.186 86 5 0 55 140 2 87 2.51e-56 171
MS.gene60717.t1 MTR_4g015960 70.270 74 15 2 68 137 10 80 2.32e-27 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60717.t1 AT4G09800 92.763 152 11 0 1 152 1 152 1.89e-105 298
MS.gene60717.t1 AT1G34030 92.763 152 11 0 1 152 1 152 1.89e-105 298
MS.gene60717.t1 AT1G22780 92.763 152 11 0 1 152 1 152 1.89e-105 298

Find 36 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAAGTTGAGAGATGATT+TGG 0.279609 1.2:-71168215 MS.gene60717:CDS
CAACTTCATGTCGAGTTGAT+TGG 0.309472 1.2:+71168228 None:intergenic
TCCCCTGCGGCCTGTAGTCT+TGG 0.394682 1.2:+71167209 None:intergenic
TTCTGTTATGCAGTAATCAC+CGG 0.438670 1.2:-71167275 MS.gene60717:intron
GTGTTGAACACAAATGTTGA+TGG 0.456259 1.2:-71168638 MS.gene60717:CDS
TACTGGGGCCTTCGTGTTCG+TGG 0.457512 1.2:-71167240 MS.gene60717:CDS
TGATTGAGGTCATTGCGAAC+TGG 0.463060 1.2:+71168603 None:intergenic
CCGGGGTTTGAGACACTACT+GGG 0.483703 1.2:-71167256 MS.gene60717:CDS
GTTCGCAATGACCTCAATCA+AGG 0.484324 1.2:-71168600 MS.gene60717:CDS
CAAGGATGGCAAATTCTCTC+AGG 0.484690 1.2:-71168257 MS.gene60717:CDS
ACCGGGGTTTGAGACACTAC+TGG 0.492415 1.2:-71167257 MS.gene60717:CDS
ATGACCTCAATCAAGGGTAT+TGG 0.493197 1.2:-71168593 MS.gene60717:CDS
GTCAATTCAAGGTTCCAGAT+TGG 0.495981 1.2:-71168303 MS.gene60717:CDS
TGGAGAGACTCAAGAAGATC+CGG 0.512286 1.2:-71168195 MS.gene60717:intron
TGCAAATCCTCGTCAATTCA+AGG 0.522315 1.2:-71168314 MS.gene60717:CDS
CCCAGTAGTGTCTCAAACCC+CGG 0.525189 1.2:+71167256 None:intergenic
GAACAGAAAGAAGGATTACA+AGG 0.525479 1.2:-71168275 MS.gene60717:CDS
TGATGTGTTATAGTCTTTGG+TGG 0.546379 1.2:-71168690 MS.gene60717:intron
TGAATTGAGTGCTGCAGAGT+TGG 0.553099 1.2:-71168356 MS.gene60717:CDS
AGAAAGAAGGATTACAAGGA+TGG 0.565673 1.2:-71168271 MS.gene60717:CDS
GGTCAGCATACCAAGACTAC+AGG 0.569467 1.2:-71167219 MS.gene60717:CDS
TACCAAGACTACAGGCCGCA+GGG 0.580402 1.2:-71167211 MS.gene60717:CDS
CTGATGTTGACATGAACAAG+AGG 0.588568 1.2:-71168538 MS.gene60717:intron
CCTACCAATACCCTTGATTG+AGG 0.590405 1.2:+71168589 None:intergenic
ATACCAAGACTACAGGCCGC+AGG 0.594524 1.2:-71167212 MS.gene60717:CDS
TCTGTTATGCAGTAATCACC+GGG 0.594587 1.2:-71167274 MS.gene60717:intron
GGCCGCAGGGGAAAGACTGT+TGG 0.608087 1.2:-71167198 MS.gene60717:CDS
GTTGGATAATATTATGACAG+TGG 0.620536 1.2:-71168338 MS.gene60717:CDS
TTCGCAATGACCTCAATCAA+GGG 0.622158 1.2:-71168599 MS.gene60717:CDS
CCTCAATCAAGGGTATTGGT+AGG 0.631523 1.2:-71168589 MS.gene60717:CDS
ACCAAGACTACAGGCCGCAG+GGG 0.654823 1.2:-71167210 MS.gene60717:CDS
TCTGGAACCTTGAATTGACG+AGG 0.669518 1.2:+71168307 None:intergenic
CTGTTATGCAGTAATCACCG+GGG 0.730953 1.2:-71167273 MS.gene60717:intron
TATGCTGACCACGAACACGA+AGG 0.739225 1.2:+71167232 None:intergenic
CACCAACAGTCTTTCCCCTG+CGG 0.740988 1.2:+71167196 None:intergenic
CGGGGTTTGAGACACTACTG+GGG 0.755067 1.2:-71167255 MS.gene60717:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCAATTCAATAATATAA+TGG - chr1.2:71168586-71168605 MS.gene60717:CDS 10.0%
!! TTTGAGTAGTAAAAAAATAA+TGG + chr1.2:71168509-71168528 None:intergenic 15.0%
!!! ATTTTTGTTGTTCAAATTAA+TGG - chr1.2:71167463-71167482 MS.gene60717:intron 15.0%
!!! TGGTTTTTTTTTTTTTTTGT+TGG - chr1.2:71167543-71167562 MS.gene60717:intron 15.0%
!!! TTTTTGTTGTTCAAATTAAT+GGG - chr1.2:71167464-71167483 MS.gene60717:intron 15.0%
!!! TTTTTTGTTGGATTTATTTA+GGG - chr1.2:71167555-71167574 MS.gene60717:intron 15.0%
!!! TTTTTTTGTTGGATTTATTT+AGG - chr1.2:71167554-71167573 MS.gene60717:intron 15.0%
!! AATAAGATGAAAACAGTTTA+TGG - chr1.2:71168397-71168416 MS.gene60717:intron 20.0%
!! AATACATAGTAAAAAATGCA+AGG + chr1.2:71168214-71168233 None:intergenic 20.0%
!! AGTTTAAATAAATCCTATCT+GGG - chr1.2:71168477-71168496 MS.gene60717:intron 20.0%
!! ATGATATTCATGTTGATATA+GGG - chr1.2:71168251-71168270 MS.gene60717:CDS 20.0%
!! TAGTTTAAATAAATCCTATC+TGG - chr1.2:71168476-71168495 MS.gene60717:intron 20.0%
!! TATGATATTCATGTTGATAT+AGG - chr1.2:71168250-71168269 MS.gene60717:CDS 20.0%
!! TTCTGTATTCAATAATACAA+TGG - chr1.2:71168529-71168548 MS.gene60717:intron 20.0%
!!! AATAATATAATGGATGTATG+TGG - chr1.2:71168596-71168615 MS.gene60717:CDS 20.0%
!!! CAATAAAATTTTAGACTGTT+TGG - chr1.2:71168301-71168320 MS.gene60717:CDS 20.0%
!!! TATTTTTGATGTTGTTGAAA+TGG - chr1.2:71167523-71167542 MS.gene60717:intron 20.0%
! AATAATACAATGGATGTATG+TGG - chr1.2:71168539-71168558 MS.gene60717:CDS 25.0%
! ACAGTTTAATTAAGCACTTA+TGG - chr1.2:71168328-71168347 MS.gene60717:CDS 25.0%
! ATCATCTTTATAGTTAACAC+AGG + chr1.2:71167806-71167825 None:intergenic 25.0%
! GAAAACAAAATACAAGTCAT+TGG + chr1.2:71168163-71168182 None:intergenic 25.0%
! TTAACATTTCAACACTATGT+AGG - chr1.2:71167860-71167879 MS.gene60717:intron 25.0%
! TTAGAACGAGAATTAACTAT+TGG - chr1.2:71167942-71167961 MS.gene60717:intron 25.0%
!! TATGATTTTTAAACGTGAGA+TGG + chr1.2:71167505-71167524 None:intergenic 25.0%
!!! ACTTGTATTTTGTTTTCTCT+TGG - chr1.2:71168166-71168185 MS.gene60717:intron 25.0%
AATCAAATGTTTCCCATAAG+CGG - chr1.2:71168093-71168112 MS.gene60717:intron 30.0%
AGATGTAGTAGTAAGATGAT+AGG - chr1.2:71167896-71167915 MS.gene60717:intron 30.0%
AGTGTTTGAAAATAGAACAG+TGG - chr1.2:71168046-71168065 MS.gene60717:intron 30.0%
ATGAAAACAGTTTATGGACA+TGG - chr1.2:71168403-71168422 MS.gene60717:intron 30.0%
ATTTGCATAAGCAAACTGTT+TGG + chr1.2:71168452-71168471 None:intergenic 30.0%
GTGTTTGAAAATAGAACAGT+GGG - chr1.2:71168047-71168066 MS.gene60717:intron 30.0%
GTTGGATAATATTATGACAG+TGG - chr1.2:71167600-71167619 MS.gene60717:intron 30.0%
TTGTGATGTGTTATAGTCTT+TGG - chr1.2:71167245-71167264 MS.gene60717:CDS 30.0%
TTTCTGTTCAAAAACCAATC+TGG + chr1.2:71167652-71167671 None:intergenic 30.0%
TTTGCATAAGCAAACTGTTT+GGG + chr1.2:71168451-71168470 None:intergenic 30.0%
!! TTGGTTTTTGAACAGAAAGA+AGG - chr1.2:71167654-71167673 MS.gene60717:intron 30.0%
!!! CTCAGTTGGTTTTTTAGTAT+AGG - chr1.2:71167831-71167850 MS.gene60717:intron 30.0%
AAAGATGATTCTCACTCAGT+TGG - chr1.2:71167817-71167836 MS.gene60717:intron 35.0%
ACAATGAAGTAGCATCTTAC+CGG + chr1.2:71167765-71167784 None:intergenic 35.0%
AGAAAGAAGGATTACAAGGA+TGG - chr1.2:71167667-71167686 MS.gene60717:intron 35.0%
ATGGATGTATGTGGAGAAAT+TGG - chr1.2:71168605-71168624 MS.gene60717:CDS 35.0%
CATGAAGTTGAGAGATGATT+TGG - chr1.2:71167723-71167742 MS.gene60717:intron 35.0%
GAACAGAAAGAAGGATTACA+AGG - chr1.2:71167663-71167682 MS.gene60717:intron 35.0%
GTGATTACTGCATAACAGAA+AGG + chr1.2:71168663-71168682 None:intergenic 35.0%
GTGTTGAACACAAATGTTGA+TGG - chr1.2:71167300-71167319 MS.gene60717:intron 35.0%
TATGTGGAGAAATTGGTCAT+TGG - chr1.2:71168612-71168631 MS.gene60717:CDS 35.0%
TGATGTGTTATAGTCTTTGG+TGG - chr1.2:71167248-71167267 MS.gene60717:CDS 35.0%
TTCTGTTATGCAGTAATCAC+CGG - chr1.2:71168663-71168682 MS.gene60717:CDS 35.0%
TTGCATAAGCAAACTGTTTG+GGG + chr1.2:71168450-71168469 None:intergenic 35.0%
! TTGTTGGATTTATTTAGGGC+TGG - chr1.2:71167559-71167578 MS.gene60717:intron 35.0%
ATAATGGCATATGCCCAGAT+AGG + chr1.2:71168493-71168512 None:intergenic 40.0%
CTGATGTTGACATGAACAAG+AGG - chr1.2:71167400-71167419 MS.gene60717:intron 40.0%
GAATTGGACCAAAAAGTAGC+TGG - chr1.2:71168130-71168149 MS.gene60717:intron 40.0%
GGAAATACGGGCAATAGAAT+TGG - chr1.2:71168114-71168133 MS.gene60717:intron 40.0%
GTCAATTCAAGGTTCCAGAT+TGG - chr1.2:71167635-71167654 MS.gene60717:intron 40.0%
GTTTCCCATAAGCGGAAATA+CGG - chr1.2:71168101-71168120 MS.gene60717:intron 40.0%
TCTGTTATGCAGTAATCACC+GGG - chr1.2:71168664-71168683 MS.gene60717:CDS 40.0%
TGCAAATCCTCGTCAATTCA+AGG - chr1.2:71167624-71167643 MS.gene60717:intron 40.0%
TTCGCAATGACCTCAATCAA+GGG - chr1.2:71167339-71167358 MS.gene60717:intron 40.0%
TTTCCCATAAGCGGAAATAC+GGG - chr1.2:71168102-71168121 MS.gene60717:intron 40.0%
! ATGACCTCAATCAAGGGTAT+TGG - chr1.2:71167345-71167364 MS.gene60717:intron 40.0%
!! CAACTTCATGTCGAGTTGAT+TGG + chr1.2:71167713-71167732 None:intergenic 40.0%
!! CGTTCTAAAGCATCAAGCAA+TGG + chr1.2:71167930-71167949 None:intergenic 40.0%
!!! GATAAGAGCCAGCTACTTTT+TGG + chr1.2:71168141-71168160 None:intergenic 40.0%
ACACGATCAAAGCAATGCAG+TGG + chr1.2:71168004-71168023 None:intergenic 45.0%
ATTGCCCGTATTTCCGCTTA+TGG + chr1.2:71168109-71168128 None:intergenic 45.0%
CAAGGATGGCAAATTCTCTC+AGG - chr1.2:71167681-71167700 MS.gene60717:intron 45.0%
CCTACCAATACCCTTGATTG+AGG + chr1.2:71167352-71167371 None:intergenic 45.0%
CTGTTATGCAGTAATCACCG+GGG - chr1.2:71168665-71168684 MS.gene60717:CDS 45.0%
GTTCGCAATGACCTCAATCA+AGG - chr1.2:71167338-71167357 MS.gene60717:intron 45.0%
TGATTGAGGTCATTGCGAAC+TGG + chr1.2:71167338-71167357 None:intergenic 45.0%
TGGAGAAATTGGTCATTGGC+TGG - chr1.2:71168616-71168635 MS.gene60717:CDS 45.0%
TGGAGAGACTCAAGAAGATC+CGG - chr1.2:71167743-71167762 MS.gene60717:intron 45.0%
TTGCCCGTATTTCCGCTTAT+GGG + chr1.2:71168108-71168127 None:intergenic 45.0%
! TCTGGAACCTTGAATTGACG+AGG + chr1.2:71167634-71167653 None:intergenic 45.0%
! TGAATTGAGTGCTGCAGAGT+TGG - chr1.2:71167582-71167601 MS.gene60717:intron 45.0%
!! CCTCAATCAAGGGTATTGGT+AGG - chr1.2:71167349-71167368 MS.gene60717:intron 45.0%
CGATCAAAGCAATGCAGTGG+TGG + chr1.2:71168001-71168020 None:intergenic 50.0%
GGTCAGCATACCAAGACTAC+AGG - chr1.2:71168719-71168738 MS.gene60717:intron 50.0%
TATGCTGACCACGAACACGA+AGG + chr1.2:71168709-71168728 None:intergenic 50.0%
ATACCAAGACTACAGGCCGC+AGG - chr1.2:71168726-71168745 MS.gene60717:intron 55.0%
CACCAACAGTCTTTCCCCTG+CGG + chr1.2:71168745-71168764 None:intergenic 55.0%
CCCAGTAGTGTCTCAAACCC+CGG + chr1.2:71168685-71168704 None:intergenic 55.0%
TACCAAGACTACAGGCCGCA+GGG - chr1.2:71168727-71168746 MS.gene60717:intron 55.0%
! ACCGGGGTTTGAGACACTAC+TGG - chr1.2:71168681-71168700 MS.gene60717:intron 55.0%
! ATGCAGTGGTGGTGCATCAG+CGG + chr1.2:71167990-71168009 None:intergenic 55.0%
! CCGGGGTTTGAGACACTACT+GGG - chr1.2:71168682-71168701 MS.gene60717:intron 55.0%
! CGGGGTTTGAGACACTACTG+GGG - chr1.2:71168683-71168702 MS.gene60717:intron 55.0%
ACCAAGACTACAGGCCGCAG+GGG - chr1.2:71168728-71168747 MS.gene60717:intron 60.0%
TACTGGGGCCTTCGTGTTCG+TGG - chr1.2:71168698-71168717 MS.gene60717:intron 60.0%
GGCCGCAGGGGAAAGACTGT+TGG - chr1.2:71168740-71168759 MS.gene60717:intron 65.0%
TCCCCTGCGGCCTGTAGTCT+TGG + chr1.2:71168732-71168751 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 71167179 71168781 71167179 ID=MS.gene60717
chr1.2 mRNA 71167179 71168781 71167179 ID=MS.gene60717.t1;Parent=MS.gene60717
chr1.2 exon 71168779 71168781 71168779 ID=MS.gene60717.t1.exon1;Parent=MS.gene60717.t1
chr1.2 CDS 71168779 71168781 71168779 ID=cds.MS.gene60717.t1;Parent=MS.gene60717.t1
chr1.2 exon 71168539 71168699 71168539 ID=MS.gene60717.t1.exon2;Parent=MS.gene60717.t1
chr1.2 CDS 71168539 71168699 71168539 ID=cds.MS.gene60717.t1;Parent=MS.gene60717.t1
chr1.2 exon 71168196 71168384 71168196 ID=MS.gene60717.t1.exon3;Parent=MS.gene60717.t1
chr1.2 CDS 71168196 71168384 71168196 ID=cds.MS.gene60717.t1;Parent=MS.gene60717.t1
chr1.2 exon 71167179 71167284 71167179 ID=MS.gene60717.t1.exon4;Parent=MS.gene60717.t1
chr1.2 CDS 71167179 71167284 71167179 ID=cds.MS.gene60717.t1;Parent=MS.gene60717.t1
Gene Sequence

>MS.gene60717

ATGGTATGTTTTCGATCTCGATTTCATCTCGATGTAGCTGCTTAATCGCTGATTCTGTTTTTTTTTTTGTGATGTGTTATAGTCTTTGGTGGCGAACGAAGAATTTCAGCACATTCTGCGTGTGTTGAACACAAATGTTGATGGAAAGCAGAAGATCCAGTTCGCAATGACCTCAATCAAGGGTATTGGTAGGAGATTCGCTAACATCTGCTGTAAGAAAGCTGATGTTGACATGAACAAGAGGTTTGTAGTTGCGATTTTGATCTCATTTTTAAAAATCAGTCATTTTTGTTGTTCAAATTAATGGGTTTCTTGAGTTTAATCCATCTCACGTTTAAAAATCATATTTTTGATGTTGTTGAAATGGTTTTTTTTTTTTTTTGTTGGATTTATTTAGGGCTGGTGAATTGAGTGCTGCAGAGTTGGATAATATTATGACAGTGGTTGCAAATCCTCGTCAATTCAAGGTTCCAGATTGGTTTTTGAACAGAAAGAAGGATTACAAGGATGGCAAATTCTCTCAGGTTGTCTCCAATCAACTCGACATGAAGTTGAGAGATGATTTGGAGAGACTCAAGAAGATCCGGTAAGATGCTACTTCATTGTTTTTTGTGTGATTGTTTGCCTGTGTTAACTATAAAGATGATTCTCACTCAGTTGGTTTTTTAGTATAGGTTTAAATTAACATTTCAACACTATGTAGGAATATCAAAATTGAGATGTAGTAGTAAGATGATAGGACTAATAACCATTGCTTGATGCTTTAGAACGAGAATTAACTATTGGAGAATCTAAAGCTCATGCACTACCGCTGATGCACCACCACTGCATTGCTTTGATCGTGTGTGATGCTTTAGCTTTCTCAATAGTGTTTGAAAATAGAACAGTGGGAAGTGTTATTAGTTTACTTGAATAATCAAATGTTTCCCATAAGCGGAAATACGGGCAATAGAATTGGACCAAAAAGTAGCTGGCTCTTATCCAATGACTTGTATTTTGTTTTCTCTTGGCTAGATAGTTTCACGTTGTCTTCCTTGCATTTTTTACTATGTATTTGCATTTTTTACTATGTATGATATTCATGTTGATATAGGGATATATGTGTCGTGAGATGAAGTAACACAATAAAATTTTAGACTGTTTGGATAAACAGTTTAATTAAGCACTTATGGCGTAAGAAATAATCATACAAGTGCTTATTTGTCAGCTGTTTTTTATAATAAGATGAAAACAGTTTATGGACATGGCATAAACTGATTCTATAAGTTCCCCAAACAGTTTGCTTATGCAAATTGATTAGTTTAAATAAATCCTATCTGGGCATATGCCATTATTTTTTTACTACTCAAATTCTGTATTCAATAATACAATGGATGTATGTGGAGTTTTTTTACTACTCAAATTTTGAATTCAATTCAATAATATAATGGATGTATGTGGAGAAATTGGTCATTGGCTGGTTAATAAAATTTTACTAACATCCTTTCTGTTATGCAGTAATCACCGGGGTTTGAGACACTACTGGGGCCTTCGTGTTCGTGGTCAGCATACCAAGACTACAGGCCGCAGGGGAAAGACTGTTGGTGTCTCAAAGAAGCGTTAA

Protein sequence

>MS.gene60717.t1

MSLVANEEFQHILRVLNTNVDGKQKIQFAMTSIKGIGRRFANICCKKADVDMNKRAGELSAAELDNIMTVVANPRQFKVPDWFLNRKKDYKDGKFSQVVSNQLDMKLRDDLERLKKIRNHRGLRHYWGLRVRGQHTKTTGRRGKTVGVSKKR