Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62695.t1 | TKY53890.1 | 100 | 118 | 0 | 0 | 1 | 118 | 35 | 152 | 4.80E-59 | 236.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62695.t1 | Q945U1 | 96.6 | 118 | 4 | 0 | 1 | 118 | 36 | 153 | 1.4e-59 | 229.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62695.t1 | G7LAJ5 | 100.0 | 118 | 0 | 0 | 1 | 118 | 37 | 154 | 3.5e-59 | 236.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene62695 | 0.81176 | 5.96E-51 | -1.69E-46 |
| MS.gene050134 | MS.gene62695 | 0.835459 | 1.66E-56 | -1.69E-46 |
| MS.gene050638 | MS.gene62695 | -0.811606 | 6.44E-51 | -1.69E-46 |
| MS.gene052664 | MS.gene62695 | 0.839998 | 1.13E-57 | -1.69E-46 |
| MS.gene052666 | MS.gene62695 | 0.930019 | 3.01E-93 | -1.69E-46 |
| MS.gene053224 | MS.gene62695 | 0.818223 | 2.19E-52 | -1.69E-46 |
| MS.gene057641 | MS.gene62695 | 0.869967 | 2.08E-66 | -1.69E-46 |
| MS.gene057643 | MS.gene62695 | 0.898294 | 6.11E-77 | -1.69E-46 |
| MS.gene060432 | MS.gene62695 | 0.84233 | 2.76E-58 | -1.69E-46 |
| MS.gene060483 | MS.gene62695 | 0.881203 | 2.92E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene28960 | MS.gene62695 | PPI |
| MS.gene003409 | MS.gene62695 | PPI |
| MS.gene62695 | MS.gene39902 | PPI |
| MS.gene62695 | MS.gene49338 | PPI |
| MS.gene052666 | MS.gene62695 | PPI |
| MS.gene62695 | MS.gene052272 | PPI |
| MS.gene62695 | MS.gene020509 | PPI |
| MS.gene62695 | MS.gene015671 | PPI |
| MS.gene62695 | MS.gene020507 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62695.t1 | MTR_8g105110 | 100.000 | 118 | 0 | 0 | 1 | 118 | 37 | 154 | 1.27e-83 | 241 |
| MS.gene62695.t1 | MTR_1g070450 | 99.153 | 118 | 1 | 0 | 1 | 118 | 35 | 152 | 1.17e-82 | 238 |
| MS.gene62695.t1 | MTR_4g075450 | 96.610 | 118 | 4 | 0 | 1 | 118 | 35 | 152 | 1.32e-79 | 231 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene62695.t1 | AT5G09500 | 89.831 | 118 | 12 | 0 | 1 | 118 | 33 | 150 | 2.34e-74 | 218 |
| MS.gene62695.t1 | AT5G09510 | 94.915 | 118 | 6 | 0 | 1 | 118 | 35 | 152 | 1.88e-71 | 210 |
| MS.gene62695.t1 | AT5G09510 | 94.915 | 118 | 6 | 0 | 1 | 118 | 1 | 118 | 2.23e-71 | 209 |
| MS.gene62695.t1 | AT1G04270 | 94.915 | 118 | 6 | 0 | 1 | 118 | 35 | 152 | 2.37e-71 | 210 |
| MS.gene62695.t1 | AT5G09490 | 83.898 | 118 | 19 | 0 | 1 | 118 | 35 | 152 | 5.63e-70 | 206 |
| MS.gene62695.t1 | AT1G04270 | 94.068 | 118 | 6 | 1 | 1 | 118 | 35 | 151 | 6.57e-69 | 204 |
| MS.gene62695.t1 | AT5G43640 | 88.983 | 118 | 13 | 0 | 1 | 118 | 32 | 149 | 9.52e-65 | 193 |
| MS.gene62695.t1 | AT5G63070 | 62.903 | 124 | 40 | 2 | 1 | 118 | 37 | 160 | 2.57e-49 | 154 |
Find 35 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATTTCAACTTGGTTAA+AGG | 0.236449 | 4.2:-1577135 | None:intergenic |
| GAGATGATTGGGAGCATTAT+TGG | 0.247005 | 4.2:+1577095 | MS.gene62695:CDS |
| CCAGGTCTACCATGCTTAAC+AGG | 0.341486 | 4.2:-1577206 | None:intergenic |
| TCAGTGCTCGTGCTCGTAGA+AGG | 0.367007 | 4.2:+1575923 | MS.gene62695:CDS |
| AGATGGGTCCTAACAGGTTC+GGG | 0.405557 | 4.2:-1577050 | None:intergenic |
| GTGAGAAGCCCGAACCTGTT+AGG | 0.409377 | 4.2:+1577042 | MS.gene62695:CDS |
| TTACGAAGATGGGTCCTAAC+AGG | 0.435557 | 4.2:-1577056 | None:intergenic |
| AAGATGGGTCCTAACAGGTT+CGG | 0.450751 | 4.2:-1577051 | None:intergenic |
| CAATGATCATGTTACGAAGA+TGG | 0.465315 | 4.2:-1577067 | None:intergenic |
| ATCATTGTACCTGAGATGAT+TGG | 0.468347 | 4.2:+1577083 | MS.gene62695:CDS |
| AGGTAATGACCGATCATCTC+AGG | 0.492365 | 4.2:-1577158 | None:intergenic |
| CCTGTTAAGCATGGTAGACC+TGG | 0.509940 | 4.2:+1577206 | MS.gene62695:CDS |
| AATGATCATGTTACGAAGAT+GGG | 0.516658 | 4.2:-1577066 | None:intergenic |
| GCATTATTGGAGTGTACAAT+GGG | 0.517140 | 4.2:+1577108 | MS.gene62695:CDS |
| ACAGGTTCGGGCTTCTCACC+AGG | 0.517899 | 4.2:-1577038 | None:intergenic |
| TTGGTGCTACTCACTCCTCC+AGG | 0.521787 | 4.2:+1577231 | MS.gene62695:CDS |
| ATAATGCTCCCAATCATCTC+AGG | 0.528225 | 4.2:-1577092 | None:intergenic |
| TCATTGTACCTGAGATGATT+GGG | 0.539509 | 4.2:+1577084 | MS.gene62695:CDS |
| GCTTACGAGTGAGACCTCTC+TGG | 0.541580 | 4.2:-1575948 | None:intergenic |
| TCTCAGGTTTGATTTCAACT+TGG | 0.546765 | 4.2:-1577142 | None:intergenic |
| AGCATTATTGGAGTGTACAA+TGG | 0.558872 | 4.2:+1577107 | MS.gene62695:CDS |
| TAGAAAAGGGAGGCACCACC+TGG | 0.561975 | 4.2:+1577020 | MS.gene62695:intron |
| TCACTTGAGAGGAATGAACC+TGG | 0.566509 | 4.2:-1577249 | None:intergenic |
| GAGTGAGTAGCACCAATACC+AGG | 0.584094 | 4.2:-1577224 | None:intergenic |
| GAAATCAAACCTGAGATGAT+CGG | 0.585387 | 4.2:+1577149 | MS.gene62695:CDS |
| AAGCATGGTAGACCTGGTAT+TGG | 0.586812 | 4.2:+1577212 | MS.gene62695:CDS |
| TCATACAAGCCTGTTAAGCA+TGG | 0.604339 | 4.2:+1577197 | MS.gene62695:CDS |
| GAGCTTAACAAGCTCATCAG+TGG | 0.607114 | 4.2:-1575899 | None:intergenic |
| AGGTCTCACTCGTAAGCCCA+TGG | 0.623580 | 4.2:+1575954 | MS.gene62695:CDS |
| AACAAGGTGATTCACTTGAG+AGG | 0.631770 | 4.2:-1577260 | None:intergenic |
| ATGAGATCGAGAATTCAGCT+AGG | 0.682732 | 4.2:-1577178 | None:intergenic |
| GGTTCGGGCTTCTCACCAGG+TGG | 0.683492 | 4.2:-1577035 | None:intergenic |
| GAATTGGTTGTAGAAAAGGG+AGG | 0.733330 | 4.2:+1577010 | MS.gene62695:intron |
| CTTGAGAGGAATGAACCTGG+AGG | 0.737810 | 4.2:-1577246 | None:intergenic |
| GCTCGTAGAAGGTTCCAGAG+AGG | 0.763484 | 4.2:+1575934 | MS.gene62695:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAATTTGTAAAATAGTAAT+TGG | + | chr4.2:1576140-1576159 | MS.gene62695:intron | 10.0% |
| !!! | AACACTTTTTTTTTTTTTTT+TGG | + | chr4.2:1576491-1576510 | MS.gene62695:intron | 10.0% |
| !!! | ATTACTATTTTACAAATTTA+AGG | - | chr4.2:1576140-1576159 | None:intergenic | 10.0% |
| !!! | TTTGTTATTTTTTTAATTTG+AGG | + | chr4.2:1576389-1576408 | MS.gene62695:intron | 10.0% |
| !! | ATTACTATTATGCAAATTTA+AGG | - | chr4.2:1576455-1576474 | None:intergenic | 15.0% |
| !!! | AAAAAAAAGTGTTCAATTTA+AGG | - | chr4.2:1576484-1576503 | None:intergenic | 15.0% |
| !!! | AAAAAAAGTGTTCAATTTAA+GGG | - | chr4.2:1576483-1576502 | None:intergenic | 15.0% |
| !!! | ATTGTTATAATTTTGTGAAT+TGG | + | chr4.2:1576994-1577013 | MS.gene62695:intron | 15.0% |
| !!! | TTTGTTATTCTTTTATTTAG+TGG | + | chr4.2:1576628-1576647 | MS.gene62695:intron | 15.0% |
| !! | ATATGCAAAATACTAACAAT+TGG | + | chr4.2:1576111-1576130 | MS.gene62695:intron | 20.0% |
| !! | TATGCAAAATACTAACAATT+GGG | + | chr4.2:1576112-1576131 | MS.gene62695:intron | 20.0% |
| !! | TTTAGTACTTAAGATAGTAA+GGG | - | chr4.2:1576962-1576981 | None:intergenic | 20.0% |
| !!! | AGGACTAACATTAAAATTTT+TGG | - | chr4.2:1576666-1576685 | None:intergenic | 20.0% |
| !!! | ATTTAACACTACCATTTTAA+AGG | + | chr4.2:1576755-1576774 | MS.gene62695:intron | 20.0% |
| !!! | GAAGTTTTTATTGATTTTGT+TGG | + | chr4.2:1576564-1576583 | MS.gene62695:intron | 20.0% |
| !!! | GTTAGTATTTTGCATATTAA+AGG | - | chr4.2:1576108-1576127 | None:intergenic | 20.0% |
| !!! | TGGTGGTTTTTTAAATTTAA+AGG | + | chr4.2:1576893-1576912 | MS.gene62695:intron | 20.0% |
| !!! | TTGTTAATTTGTGAATTTGA+GGG | + | chr4.2:1576922-1576941 | MS.gene62695:intron | 20.0% |
| !!! | TTTGTTAATTTGTGAATTTG+AGG | + | chr4.2:1576921-1576940 | MS.gene62695:intron | 20.0% |
| !!! | TTTTAGTACTTAAGATAGTA+AGG | - | chr4.2:1576963-1576982 | None:intergenic | 20.0% |
| ! | AACATGAAACCATTAAAATG+GGG | - | chr4.2:1576231-1576250 | None:intergenic | 25.0% |
| ! | AAGGAACTAAATTGATATCT+TGG | - | chr4.2:1576436-1576455 | None:intergenic | 25.0% |
| ! | AATAGCGATATTTAGGTATA+GGG | + | chr4.2:1576859-1576878 | MS.gene62695:intron | 25.0% |
| ! | ACTTCCATATAAACTTGAAA+AGG | - | chr4.2:1576549-1576568 | None:intergenic | 25.0% |
| ! | AGTAATTGGATACCAAAAAT+TGG | + | chr4.2:1576154-1576173 | MS.gene62695:intron | 25.0% |
| ! | GAACATGAAACCATTAAAAT+GGG | - | chr4.2:1576232-1576251 | None:intergenic | 25.0% |
| ! | TGAACATGAAACCATTAAAA+TGG | - | chr4.2:1576233-1576252 | None:intergenic | 25.0% |
| !! | AAACAACAATCTTAGCATTT+TGG | + | chr4.2:1576065-1576084 | MS.gene62695:intron | 25.0% |
| !! | AACAACAATCTTAGCATTTT+GGG | + | chr4.2:1576066-1576085 | MS.gene62695:intron | 25.0% |
| !! | ATATGACATTGTCCAATTTT+TGG | - | chr4.2:1576169-1576188 | None:intergenic | 25.0% |
| !! | GTGCTAATTTGCAAATATAA+AGG | - | chr4.2:1576686-1576705 | None:intergenic | 25.0% |
| !! | TTTGATTTCAACTTGGTTAA+AGG | - | chr4.2:1577138-1577157 | None:intergenic | 25.0% |
| !!! | CTCTTGTTGATTATTGATTT+TGG | + | chr4.2:1576302-1576321 | MS.gene62695:intron | 25.0% |
| !!! | CTGAAGATATATTTTGATTG+AGG | + | chr4.2:1576335-1576354 | MS.gene62695:intron | 25.0% |
| !!! | GAAATTGATTGCTGTTTTTA+GGG | + | chr4.2:1576361-1576380 | MS.gene62695:intron | 25.0% |
| !!! | TATGCCTTTTCAAGTTTATA+TGG | + | chr4.2:1576542-1576561 | MS.gene62695:intron | 25.0% |
| !!! | TGTTGATTATTGATTTTGGT+GGG | + | chr4.2:1576306-1576325 | MS.gene62695:intron | 25.0% |
| !!! | TTGTTGATTATTGATTTTGG+TGG | + | chr4.2:1576305-1576324 | MS.gene62695:intron | 25.0% |
| AAGGCATATAACCTTCAAAA+TGG | - | chr4.2:1576530-1576549 | None:intergenic | 30.0% | |
| AATGATCATGTTACGAAGAT+GGG | - | chr4.2:1577069-1577088 | None:intergenic | 30.0% | |
| CTTACTGAATAGCGATATTT+AGG | + | chr4.2:1576852-1576871 | MS.gene62695:intron | 30.0% | |
| GAATAGCGATATTTAGGTAT+AGG | + | chr4.2:1576858-1576877 | MS.gene62695:intron | 30.0% | |
| TGTGAATTGGTTGTAGAAAA+GGG | + | chr4.2:1577007-1577026 | MS.gene62695:intron | 30.0% | |
| TTGTGAATTGGTTGTAGAAA+AGG | + | chr4.2:1577006-1577025 | MS.gene62695:intron | 30.0% | |
| ! | ACGCATGTAAACCTTTAAAA+TGG | - | chr4.2:1576769-1576788 | None:intergenic | 30.0% |
| ! | ACTTTAGTACCCCATTTTAA+TGG | + | chr4.2:1576219-1576238 | MS.gene62695:intron | 30.0% |
| ! | TTAAAATGGGGTACTAAAGT+CGG | - | chr4.2:1576219-1576238 | None:intergenic | 30.0% |
| !!! | CGAAATTGATTGCTGTTTTT+AGG | + | chr4.2:1576360-1576379 | MS.gene62695:intron | 30.0% |
| AGCATTATTGGAGTGTACAA+TGG | + | chr4.2:1577107-1577126 | MS.gene62695:CDS | 35.0% | |
| ATAGGGACTAAATTGATTGG+TGG | + | chr4.2:1576876-1576895 | MS.gene62695:intron | 35.0% | |
| ATCATTGTACCTGAGATGAT+TGG | + | chr4.2:1577083-1577102 | MS.gene62695:CDS | 35.0% | |
| CAATGATCATGTTACGAAGA+TGG | - | chr4.2:1577070-1577089 | None:intergenic | 35.0% | |
| GAAATCAAACCTGAGATGAT+CGG | + | chr4.2:1577149-1577168 | MS.gene62695:CDS | 35.0% | |
| GCATATAACCTTCAAAATGG+TGG | - | chr4.2:1576527-1576546 | None:intergenic | 35.0% | |
| GCATTATTGGAGTGTACAAT+GGG | + | chr4.2:1577108-1577127 | MS.gene62695:CDS | 35.0% | |
| GGTATAGGGACTAAATTGAT+TGG | + | chr4.2:1576873-1576892 | MS.gene62695:intron | 35.0% | |
| GTGACTCAGTGTTCAAATTA+AGG | - | chr4.2:1576716-1576735 | None:intergenic | 35.0% | |
| TCATTGTACCTGAGATGATT+GGG | + | chr4.2:1577084-1577103 | MS.gene62695:CDS | 35.0% | |
| ! | CAAAGTTACCACCATTTTGA+AGG | + | chr4.2:1576516-1576535 | MS.gene62695:intron | 35.0% |
| ! | TCTCAGGTTTGATTTCAACT+TGG | - | chr4.2:1577145-1577164 | None:intergenic | 35.0% |
| ATAATGCTCCCAATCATCTC+AGG | - | chr4.2:1577095-1577114 | None:intergenic | 40.0% | |
| ATGAGATCGAGAATTCAGCT+AGG | - | chr4.2:1577181-1577200 | None:intergenic | 40.0% | |
| CATCAAAAAGCTTCGCAAAG+CGG | + | chr4.2:1575981-1576000 | MS.gene62695:CDS | 40.0% | |
| GAATTGGTTGTAGAAAAGGG+AGG | + | chr4.2:1577010-1577029 | MS.gene62695:intron | 40.0% | |
| TCATACAAGCCTGTTAAGCA+TGG | + | chr4.2:1577197-1577216 | MS.gene62695:CDS | 40.0% | |
| ! | AAGCTTTTTGATGAGAGCCA+TGG | - | chr4.2:1575974-1575993 | None:intergenic | 40.0% |
| ! | AGCTTTTTGATGAGAGCCAT+GGG | - | chr4.2:1575973-1575992 | None:intergenic | 40.0% |
| !! | GAGATGATTGGGAGCATTAT+TGG | + | chr4.2:1577095-1577114 | MS.gene62695:CDS | 40.0% |
| AAGATGGGTCCTAACAGGTT+CGG | - | chr4.2:1577054-1577073 | None:intergenic | 45.0% | |
| AAGCATGGTAGACCTGGTAT+TGG | + | chr4.2:1577212-1577231 | MS.gene62695:CDS | 45.0% | |
| AGGTAATGACCGATCATCTC+AGG | - | chr4.2:1577161-1577180 | None:intergenic | 45.0% | |
| GAGCTTAACAAGCTCATCAG+TGG | - | chr4.2:1575902-1575921 | None:intergenic | 45.0% | |
| TCACTTGAGAGGAATGAACC+TGG | - | chr4.2:1577252-1577271 | None:intergenic | 45.0% | |
| TTACGAAGATGGGTCCTAAC+AGG | - | chr4.2:1577059-1577078 | None:intergenic | 45.0% | |
| !! | ATAAATAAAAGAAATAAAAA+TGG | - | chr4.2:1576022-1576041 | None:intergenic | 5.0% |
| !! | TAAATAAAAGAAATAAAAAT+GGG | - | chr4.2:1576021-1576040 | None:intergenic | 5.0% |
| AGATGGGTCCTAACAGGTTC+GGG | - | chr4.2:1577053-1577072 | None:intergenic | 50.0% | |
| CCAGGTCTACCATGCTTAAC+AGG | - | chr4.2:1577209-1577228 | None:intergenic | 50.0% | |
| CCTGTTAAGCATGGTAGACC+TGG | + | chr4.2:1577206-1577225 | MS.gene62695:CDS | 50.0% | |
| CTTGAGAGGAATGAACCTGG+AGG | - | chr4.2:1577249-1577268 | None:intergenic | 50.0% | |
| GAGTGAGTAGCACCAATACC+AGG | - | chr4.2:1577227-1577246 | None:intergenic | 50.0% | |
| AGGTCTCACTCGTAAGCCCA+TGG | + | chr4.2:1575954-1575973 | MS.gene62695:CDS | 55.0% | |
| GCTCGTAGAAGGTTCCAGAG+AGG | + | chr4.2:1575934-1575953 | MS.gene62695:CDS | 55.0% | |
| GCTTACGAGTGAGACCTCTC+TGG | - | chr4.2:1575951-1575970 | None:intergenic | 55.0% | |
| GTGAGAAGCCCGAACCTGTT+AGG | + | chr4.2:1577042-1577061 | MS.gene62695:CDS | 55.0% | |
| TCAGTGCTCGTGCTCGTAGA+AGG | + | chr4.2:1575923-1575942 | MS.gene62695:CDS | 55.0% | |
| !! | TAGAAAAGGGAGGCACCACC+TGG | + | chr4.2:1577020-1577039 | MS.gene62695:intron | 55.0% |
| !! | TTGGTGCTACTCACTCCTCC+AGG | + | chr4.2:1577231-1577250 | MS.gene62695:CDS | 55.0% |
| ACAGGTTCGGGCTTCTCACC+AGG | - | chr4.2:1577041-1577060 | None:intergenic | 60.0% | |
| GGTTCGGGCTTCTCACCAGG+TGG | - | chr4.2:1577038-1577057 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 1575895 | 1577271 | 1575895 | ID=MS.gene62695 |
| chr4.2 | mRNA | 1575895 | 1577271 | 1575895 | ID=MS.gene62695.t1;Parent=MS.gene62695 |
| chr4.2 | exon | 1575895 | 1576002 | 1575895 | ID=MS.gene62695.t1.exon1;Parent=MS.gene62695.t1 |
| chr4.2 | CDS | 1575895 | 1576002 | 1575895 | ID=cds.MS.gene62695.t1;Parent=MS.gene62695.t1 |
| chr4.2 | exon | 1577023 | 1577271 | 1577023 | ID=MS.gene62695.t1.exon2;Parent=MS.gene62695.t1 |
| chr4.2 | CDS | 1577023 | 1577271 | 1577023 | ID=cds.MS.gene62695.t1;Parent=MS.gene62695.t1 |
| Gene Sequence |
| Protein sequence |
