Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88892.t1 | XP_003592049.1 | 100 | 152 | 0 | 0 | 1 | 152 | 1 | 152 | 1.40E-79 | 305.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88892.t1 | P34788 | 92.8 | 152 | 11 | 0 | 1 | 152 | 1 | 152 | 3.3e-77 | 288.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88892.t1 | B7FMJ8 | 100.0 | 152 | 0 | 0 | 1 | 152 | 1 | 152 | 1.0e-79 | 305.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050252 | MS.gene88892 | 0.816061 | 6.71E-52 | -1.69E-46 |
| MS.gene050290 | MS.gene88892 | 0.872088 | 4.16E-67 | -1.69E-46 |
| MS.gene051963 | MS.gene88892 | 0.801059 | 1.08E-48 | -1.69E-46 |
| MS.gene054937 | MS.gene88892 | 0.870292 | 1.63E-66 | -1.69E-46 |
| MS.gene056075 | MS.gene88892 | 0.815238 | 1.02E-51 | -1.69E-46 |
| MS.gene056917 | MS.gene88892 | 0.803798 | 2.94E-49 | -1.69E-46 |
| MS.gene057052 | MS.gene88892 | 0.845177 | 4.78E-59 | -1.69E-46 |
| MS.gene057092 | MS.gene88892 | 0.830346 | 3.09E-55 | -1.69E-46 |
| MS.gene057235 | MS.gene88892 | 0.813396 | 2.61E-51 | -1.69E-46 |
| MS.gene058354 | MS.gene88892 | 0.810682 | 1.02E-50 | -1.69E-46 |
| MS.gene059893 | MS.gene88892 | 0.842732 | 2.16E-58 | -1.69E-46 |
| MS.gene060574 | MS.gene88892 | 0.831255 | 1.85E-55 | -1.69E-46 |
| MS.gene060856 | MS.gene88892 | 0.902131 | 1.33E-78 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88892.t1 | MTR_1g098170 | 100.000 | 152 | 0 | 0 | 1 | 152 | 1 | 152 | 6.47e-112 | 314 |
| MS.gene88892.t1 | MTR_1g098220 | 100.000 | 152 | 0 | 0 | 1 | 152 | 1 | 152 | 6.47e-112 | 314 |
| MS.gene88892.t1 | MTR_6g043210 | 98.684 | 152 | 2 | 0 | 1 | 152 | 1 | 152 | 6.70e-111 | 311 |
| MS.gene88892.t1 | MTR_1g492710 | 97.945 | 146 | 3 | 0 | 1 | 146 | 794 | 939 | 1.21e-97 | 303 |
| MS.gene88892.t1 | MTR_1g098220 | 99.160 | 119 | 1 | 0 | 1 | 119 | 1 | 119 | 2.71e-84 | 243 |
| MS.gene88892.t1 | MTR_5g027650 | 95.098 | 102 | 5 | 0 | 39 | 140 | 11 | 112 | 7.93e-69 | 203 |
| MS.gene88892.t1 | MTR_5g027580 | 93.137 | 102 | 7 | 0 | 39 | 140 | 48 | 149 | 7.08e-68 | 202 |
| MS.gene88892.t1 | MTR_5g027670 | 94.382 | 89 | 5 | 0 | 52 | 140 | 1 | 89 | 2.79e-58 | 176 |
| MS.gene88892.t1 | MTR_5g027615 | 94.382 | 89 | 5 | 0 | 52 | 140 | 1 | 89 | 7.00e-58 | 175 |
| MS.gene88892.t1 | MTR_5g027605 | 94.382 | 89 | 5 | 0 | 52 | 140 | 1 | 89 | 7.00e-58 | 175 |
| MS.gene88892.t1 | MTR_5g027640 | 94.382 | 89 | 5 | 0 | 52 | 140 | 1 | 89 | 7.00e-58 | 175 |
| MS.gene88892.t1 | MTR_5g027600 | 94.382 | 89 | 5 | 0 | 52 | 140 | 1 | 89 | 7.00e-58 | 175 |
| MS.gene88892.t1 | MTR_1005s0010 | 94.186 | 86 | 5 | 0 | 55 | 140 | 2 | 87 | 2.51e-56 | 171 |
| MS.gene88892.t1 | MTR_4g015960 | 70.270 | 74 | 15 | 2 | 68 | 137 | 10 | 80 | 2.32e-27 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88892.t1 | AT4G09800 | 92.763 | 152 | 11 | 0 | 1 | 152 | 1 | 152 | 1.89e-105 | 298 |
| MS.gene88892.t1 | AT1G34030 | 92.763 | 152 | 11 | 0 | 1 | 152 | 1 | 152 | 1.89e-105 | 298 |
| MS.gene88892.t1 | AT1G22780 | 92.763 | 152 | 11 | 0 | 1 | 152 | 1 | 152 | 1.89e-105 | 298 |
Find 36 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCTTCATGTCAAGTTGAT+TGG | 0.350481 | 1.1:-70636692 | None:intergenic |
| CATGAAGCTGAGGGATGATT+TGG | 0.353151 | 1.1:+70636705 | MS.gene88892:CDS |
| ATCGTGGATTGAGGCACTAC+TGG | 0.374036 | 1.1:+70637563 | MS.gene88892:CDS |
| TCCTCTGCGGCCAGTAGTCT+TGG | 0.415117 | 1.1:-70637611 | None:intergenic |
| TACTGGGGCCTTCGTGTTCG+TGG | 0.449083 | 1.1:+70637580 | MS.gene88892:CDS |
| GTGTTGAACACAAATGTTGA+TGG | 0.456259 | 1.1:+70609954 | MS.gene88892:CDS |
| TGATTGAGGTCATTGCGAAC+TGG | 0.463060 | 1.1:-70609989 | None:intergenic |
| GTTCGCAATGACCTCAATCA+AGG | 0.484324 | 1.1:+70609992 | MS.gene88892:CDS |
| ATGACCTCAATCAAGGGTAT+TGG | 0.493197 | 1.1:+70609999 | MS.gene88892:CDS |
| GTCAATTCAAGGTTCCAGAT+TGG | 0.495981 | 1.1:+70636617 | MS.gene88892:CDS |
| CGCTAACATCTGCTGTAAGA+AGG | 0.503592 | 1.1:+70610031 | MS.gene88892:CDS |
| TGCGAATCCTCGTCAATTCA+AGG | 0.503849 | 1.1:+70636606 | MS.gene88892:CDS |
| TGGAGAGACTCAAGAAGATC+CGG | 0.515884 | 1.1:+70636725 | MS.gene88892:CDS |
| TCGTGGATTGAGGCACTACT+GGG | 0.516130 | 1.1:+70637564 | MS.gene88892:CDS |
| GAACAGAAAGAAGGATTACA+AGG | 0.525479 | 1.1:+70636645 | MS.gene88892:CDS |
| TGATGTGTTATAGTCTTTGG+TGG | 0.546379 | 1.1:+70609902 | MS.gene88892:intron |
| GGTCAGCATACCAAGACTAC+TGG | 0.555192 | 1.1:+70637601 | MS.gene88892:CDS |
| AGAAAGAAGGATTACAAGGA+TGG | 0.565673 | 1.1:+70636649 | MS.gene88892:CDS |
| TGAATTGAGTGCCGCAGAGT+TGG | 0.583506 | 1.1:+70636564 | MS.gene88892:CDS |
| CCTACCAATACCCTTGATTG+AGG | 0.590405 | 1.1:-70610003 | None:intergenic |
| ATCAACTTGACATGAAGCTG+AGG | 0.604543 | 1.1:+70636695 | MS.gene88892:CDS |
| ACCAAGACTACTGGCCGCAG+AGG | 0.620084 | 1.1:+70637610 | MS.gene88892:CDS |
| GTTGGATAATATTATGACAG+TGG | 0.620536 | 1.1:+70636582 | MS.gene88892:CDS |
| TTCGCAATGACCTCAATCAA+GGG | 0.622158 | 1.1:+70609993 | MS.gene88892:CDS |
| GGCCGCAGAGGAAAGACAGT+TGG | 0.623790 | 1.1:+70637622 | MS.gene88892:CDS |
| CCTCAATCAAGGGTATTGGT+AGG | 0.631523 | 1.1:+70610003 | MS.gene88892:CDS |
| TCAACTTGACATGAAGCTGA+GGG | 0.637423 | 1.1:+70636696 | MS.gene88892:CDS |
| GCAGTAATCATCGTGGATTG+AGG | 0.637806 | 1.1:+70637554 | MS.gene88892:intron |
| CTGTTATGCAGTAATCATCG+TGG | 0.641883 | 1.1:+70637547 | MS.gene88892:intron |
| CCTCTTGTTCATGTCAACAT+CGG | 0.651019 | 1.1:-70610054 | None:intergenic |
| CCGATGTTGACATGAACAAG+AGG | 0.658386 | 1.1:+70610054 | MS.gene88892:CDS |
| TCTGGAACCTTGAATTGACG+AGG | 0.669518 | 1.1:-70636613 | None:intergenic |
| CATAATATTATCCAACTCTG+CGG | 0.698001 | 1.1:-70636575 | None:intergenic |
| CACCAACTGTCTTTCCTCTG+CGG | 0.707516 | 1.1:-70637624 | None:intergenic |
| CGTGGATTGAGGCACTACTG+GGG | 0.709284 | 1.1:+70637565 | MS.gene88892:CDS |
| TATGCTGACCACGAACACGA+AGG | 0.739225 | 1.1:-70637588 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 70609833 | 70637663 | 70609833 | ID=MS.gene88892 |
| chr1.1 | mRNA | 70609833 | 70637663 | 70609833 | ID=MS.gene88892.t1;Parent=MS.gene88892 |
| chr1.1 | exon | 70609833 | 70609835 | 70609833 | ID=MS.gene88892.t1.exon1;Parent=MS.gene88892.t1 |
| chr1.1 | CDS | 70609833 | 70609835 | 70609833 | ID=cds.MS.gene88892.t1;Parent=MS.gene88892.t1 |
| chr1.1 | exon | 70609915 | 70610075 | 70609915 | ID=MS.gene88892.t1.exon2;Parent=MS.gene88892.t1 |
| chr1.1 | CDS | 70609915 | 70610075 | 70609915 | ID=cds.MS.gene88892.t1;Parent=MS.gene88892.t1 |
| chr1.1 | exon | 70636558 | 70636746 | 70636558 | ID=MS.gene88892.t1.exon3;Parent=MS.gene88892.t1 |
| chr1.1 | CDS | 70636558 | 70636746 | 70636558 | ID=cds.MS.gene88892.t1;Parent=MS.gene88892.t1 |
| chr1.1 | exon | 70637558 | 70637663 | 70637558 | ID=MS.gene88892.t1.exon4;Parent=MS.gene88892.t1 |
| chr1.1 | CDS | 70637558 | 70637663 | 70637558 | ID=cds.MS.gene88892.t1;Parent=MS.gene88892.t1 |
| Gene Sequence |
| Protein sequence |
