Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024711.t1 | XP_003610947.1 | 99 | 693 | 7 | 0 | 1 | 693 | 1 | 693 | 0.00E+00 | 1467.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024711.t1 | Q9SXV6 | 88.0 | 693 | 83 | 0 | 1 | 693 | 1 | 693 | 0.0e+00 | 1341.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024711.t1 | G7K980 | 99.0 | 693 | 7 | 0 | 1 | 693 | 1 | 693 | 0.0e+00 | 1467.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene024711 | MS.gene87166 | PPI |
| MS.gene024711 | MS.gene053114 | PPI |
| MS.gene024711 | MS.gene000594 | PPI |
| MS.gene011204 | MS.gene024711 | PPI |
| MS.gene98129 | MS.gene024711 | PPI |
| MS.gene024711 | MS.gene98126 | PPI |
| MS.gene024711 | MS.gene034308 | PPI |
| MS.gene024711 | MS.gene50096 | PPI |
| MS.gene024711 | MS.gene034307 | PPI |
| MS.gene024711 | MS.gene37340 | PPI |
| MS.gene024711 | MS.gene07774 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024711.t1 | MTR_5g008810 | 98.990 | 693 | 7 | 0 | 1 | 693 | 1 | 693 | 0.0 | 1434 |
| MS.gene024711.t1 | MTR_7g062020 | 63.688 | 705 | 244 | 4 | 1 | 693 | 1 | 705 | 0.0 | 956 |
| MS.gene024711.t1 | MTR_6g088140 | 63.845 | 697 | 229 | 3 | 1 | 693 | 1 | 678 | 0.0 | 955 |
| MS.gene024711.t1 | MTR_6g093100 | 63.062 | 712 | 244 | 5 | 1 | 693 | 1 | 712 | 0.0 | 951 |
| MS.gene024711.t1 | MTR_6g088140 | 63.189 | 671 | 224 | 3 | 1 | 667 | 1 | 652 | 0.0 | 912 |
| MS.gene024711.t1 | MTR_6g093180 | 59.510 | 694 | 278 | 3 | 1 | 693 | 1 | 692 | 0.0 | 892 |
| MS.gene024711.t1 | MTR_4g005270 | 58.046 | 696 | 288 | 4 | 1 | 693 | 1 | 695 | 0.0 | 871 |
| MS.gene024711.t1 | MTR_8g016975 | 57.328 | 696 | 293 | 4 | 1 | 693 | 1 | 695 | 0.0 | 868 |
| MS.gene024711.t1 | MTR_4g005190 | 57.328 | 696 | 293 | 4 | 1 | 693 | 1 | 695 | 0.0 | 865 |
| MS.gene024711.t1 | MTR_0013s0230 | 57.676 | 697 | 290 | 5 | 1 | 693 | 1 | 696 | 0.0 | 855 |
| MS.gene024711.t1 | MTR_6g034985 | 56.916 | 694 | 296 | 3 | 1 | 693 | 1 | 692 | 0.0 | 847 |
| MS.gene024711.t1 | MTR_4g005190 | 57.377 | 671 | 282 | 4 | 1 | 668 | 1 | 670 | 0.0 | 839 |
| MS.gene024711.t1 | MTR_6g035010 | 55.620 | 694 | 305 | 3 | 1 | 693 | 1 | 692 | 0.0 | 827 |
| MS.gene024711.t1 | MTR_8g016960 | 54.232 | 697 | 308 | 6 | 1 | 693 | 1 | 690 | 0.0 | 812 |
| MS.gene024711.t1 | MTR_8g017020 | 56.811 | 646 | 274 | 4 | 50 | 693 | 19 | 661 | 0.0 | 798 |
| MS.gene024711.t1 | MTR_7g034535 | 58.439 | 628 | 257 | 4 | 69 | 693 | 5 | 631 | 0.0 | 786 |
| MS.gene024711.t1 | MTR_3g436160 | 57.749 | 613 | 252 | 5 | 83 | 693 | 27 | 634 | 0.0 | 742 |
| MS.gene024711.t1 | MTR_8g016940 | 49.756 | 205 | 101 | 1 | 489 | 693 | 2 | 204 | 4.48e-64 | 214 |
| MS.gene024711.t1 | MTR_8g017040 | 51.429 | 70 | 33 | 1 | 1 | 69 | 1 | 70 | 4.32e-17 | 78.6 |
| MS.gene024711.t1 | MTR_3g436170 | 52.239 | 67 | 31 | 1 | 1 | 66 | 1 | 67 | 1.15e-15 | 73.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene024711.t1 | AT2G07050 | 80.375 | 693 | 136 | 0 | 1 | 693 | 1 | 693 | 0.0 | 1220 |
| MS.gene024711.t1 | AT2G07050 | 82.862 | 601 | 103 | 0 | 93 | 693 | 2 | 602 | 0.0 | 1085 |
| MS.gene024711.t1 | AT3G45130 | 65.086 | 696 | 237 | 3 | 1 | 693 | 1 | 693 | 0.0 | 918 |
| MS.gene024711.t1 | AT3G45130 | 65.086 | 696 | 237 | 3 | 1 | 693 | 1 | 693 | 0.0 | 918 |
| MS.gene024711.t1 | AT3G45130 | 65.086 | 696 | 237 | 3 | 1 | 693 | 1 | 693 | 0.0 | 918 |
| MS.gene024711.t1 | AT3G45130 | 65.086 | 696 | 237 | 3 | 1 | 693 | 1 | 693 | 0.0 | 918 |
| MS.gene024711.t1 | AT3G45130 | 65.086 | 696 | 237 | 3 | 1 | 693 | 1 | 693 | 0.0 | 918 |
| MS.gene024711.t1 | AT1G78960 | 56.671 | 697 | 297 | 4 | 1 | 693 | 1 | 696 | 0.0 | 849 |
| MS.gene024711.t1 | AT1G78960 | 56.671 | 697 | 297 | 4 | 1 | 693 | 1 | 696 | 0.0 | 849 |
| MS.gene024711.t1 | AT1G78960 | 56.671 | 697 | 297 | 4 | 1 | 693 | 1 | 696 | 0.0 | 849 |
| MS.gene024711.t1 | AT1G78960 | 56.671 | 697 | 297 | 4 | 1 | 693 | 1 | 696 | 0.0 | 848 |
| MS.gene024711.t1 | AT1G78955 | 57.245 | 697 | 293 | 4 | 1 | 693 | 1 | 696 | 0.0 | 847 |
| MS.gene024711.t1 | AT1G78950 | 57.245 | 697 | 293 | 4 | 1 | 693 | 1 | 696 | 0.0 | 841 |
| MS.gene024711.t1 | AT1G78950 | 57.245 | 697 | 293 | 4 | 1 | 693 | 10 | 705 | 0.0 | 841 |
| MS.gene024711.t1 | AT1G66960 | 53.372 | 697 | 320 | 4 | 1 | 693 | 10 | 705 | 0.0 | 800 |
| MS.gene024711.t1 | AT1G66960 | 53.372 | 697 | 320 | 4 | 1 | 693 | 1 | 696 | 0.0 | 799 |
| MS.gene024711.t1 | AT1G66960 | 53.372 | 697 | 320 | 4 | 1 | 693 | 1 | 696 | 0.0 | 799 |
| MS.gene024711.t1 | AT1G66960 | 53.372 | 697 | 320 | 4 | 1 | 693 | 1 | 696 | 0.0 | 799 |
| MS.gene024711.t1 | AT1G78970 | 54.023 | 696 | 314 | 4 | 1 | 693 | 1 | 693 | 0.0 | 787 |
| MS.gene024711.t1 | AT1G78970 | 54.023 | 696 | 314 | 4 | 1 | 693 | 1 | 693 | 0.0 | 787 |
| MS.gene024711.t1 | AT1G78970 | 54.023 | 696 | 314 | 4 | 1 | 693 | 1 | 693 | 0.0 | 787 |
| MS.gene024711.t1 | AT1G78955 | 58.320 | 631 | 260 | 3 | 65 | 693 | 2 | 631 | 0.0 | 782 |
| MS.gene024711.t1 | AT1G78955 | 58.320 | 631 | 260 | 3 | 65 | 693 | 2 | 631 | 0.0 | 781 |
| MS.gene024711.t1 | AT1G78955 | 58.940 | 604 | 245 | 3 | 65 | 666 | 2 | 604 | 0.0 | 759 |
| MS.gene024711.t1 | AT5G36150 | 49.138 | 696 | 349 | 4 | 1 | 692 | 1 | 695 | 0.0 | 743 |
| MS.gene024711.t1 | AT5G36150 | 49.138 | 696 | 349 | 4 | 1 | 692 | 1 | 695 | 0.0 | 743 |
| MS.gene024711.t1 | AT1G78500 | 49.145 | 702 | 346 | 7 | 1 | 692 | 1 | 701 | 0.0 | 730 |
| MS.gene024711.t1 | AT1G78500 | 49.145 | 702 | 346 | 7 | 1 | 692 | 1 | 701 | 0.0 | 730 |
| MS.gene024711.t1 | AT4G15370 | 48.143 | 700 | 347 | 6 | 1 | 692 | 1 | 692 | 0.0 | 714 |
| MS.gene024711.t1 | AT4G15340 | 48.645 | 701 | 350 | 6 | 1 | 692 | 1 | 700 | 0.0 | 712 |
| MS.gene024711.t1 | AT4G15370 | 47.937 | 703 | 347 | 7 | 1 | 692 | 1 | 695 | 0.0 | 709 |
| MS.gene024711.t1 | AT5G48010 | 47.076 | 701 | 361 | 5 | 1 | 692 | 1 | 700 | 0.0 | 707 |
| MS.gene024711.t1 | AT1G78960 | 58.974 | 546 | 221 | 3 | 150 | 693 | 1 | 545 | 0.0 | 699 |
| MS.gene024711.t1 | AT5G48010 | 46.648 | 701 | 356 | 6 | 1 | 692 | 1 | 692 | 0.0 | 696 |
| MS.gene024711.t1 | AT5G42600 | 46.915 | 697 | 364 | 5 | 1 | 692 | 1 | 696 | 0.0 | 671 |
| MS.gene024711.t1 | AT1G78955 | 59.579 | 522 | 208 | 3 | 174 | 693 | 1 | 521 | 0.0 | 652 |
| MS.gene024711.t1 | AT3G29255 | 43.678 | 696 | 327 | 8 | 2 | 692 | 6 | 641 | 0.0 | 593 |
| MS.gene024711.t1 | AT4G15340 | 47.887 | 568 | 286 | 6 | 1 | 559 | 1 | 567 | 0.0 | 563 |
| MS.gene024711.t1 | AT1G78480 | 37.143 | 140 | 82 | 3 | 58 | 194 | 36 | 172 | 1.64e-19 | 87.8 |
Find 190 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCACTTGGTTTGGGGTAAA+AGG | 0.154236 | 5.4:-3630549 | MS.gene024711:CDS |
| TGTTCAGGTGTATTCAAATT+TGG | 0.159318 | 5.4:-3629198 | MS.gene024711:intron |
| TCACTTATGGCACTTGGTTT+GGG | 0.188803 | 5.4:-3630557 | MS.gene024711:CDS |
| ACTAACCTAATTGAAGAATT+TGG | 0.231134 | 5.4:-3632954 | MS.gene024711:CDS |
| CACTCGTGCAAGATATTCTT+TGG | 0.236923 | 5.4:-3633619 | MS.gene024711:CDS |
| AACTTGAAAGCCTCTGAATT+TGG | 0.237029 | 5.4:+3633282 | None:intergenic |
| TTCACTTATGGCACTTGGTT+TGG | 0.237239 | 5.4:-3630558 | MS.gene024711:CDS |
| TCAAGAATTACCTTATTTAC+AGG | 0.242278 | 5.4:+3633464 | None:intergenic |
| TGGGGAGTTTGCTTCACTTA+TGG | 0.245949 | 5.4:-3630570 | MS.gene024711:CDS |
| TTCAGGCTTTGTCAACATTT+AGG | 0.256339 | 5.4:-3630875 | MS.gene024711:CDS |
| TTGGACCAAATTCTTCAATT+AGG | 0.261321 | 5.4:+3632949 | None:intergenic |
| CAAGGATCCATGATTATCTA+TGG | 0.267663 | 5.4:-3633251 | MS.gene024711:CDS |
| AAATGTCATTATCTCATTAC+AGG | 0.283951 | 5.4:-3632540 | MS.gene024711:intron |
| CAAGGCCAATTCATGAAAAT+AGG | 0.293099 | 5.4:+3633572 | None:intergenic |
| GATAATATTGTTGGTGTGAT+TGG | 0.294184 | 5.4:+3634271 | None:intergenic |
| TATTTCTTCCCGACGATGCC+TGG | 0.295572 | 5.4:+3630842 | None:intergenic |
| TTGACGAACGGTTTCGATAT+CGG | 0.309423 | 5.4:+3635602 | None:intergenic |
| GCCAATCCTGCTGAAACTTT+TGG | 0.309739 | 5.4:-3631051 | MS.gene024711:CDS |
| TTCCTTCATCGGATTCTCTT+TGG | 0.323424 | 5.4:+3635515 | None:intergenic |
| GCAGCTCCCATCTGGTGGTT+GGG | 0.324085 | 5.4:-3630454 | MS.gene024711:CDS |
| TGCTAACCAGGTATGGTTCT+TGG | 0.326714 | 5.4:-3630590 | MS.gene024711:intron |
| TTGCATAGGTCCTGTAAATA+AGG | 0.328833 | 5.4:-3633474 | MS.gene024711:intron |
| AAACGATGGAGAAGGGGATA+TGG | 0.329008 | 5.4:-3634761 | MS.gene024711:CDS |
| TTCTTACCTGATGATTATAA+AGG | 0.337163 | 5.4:+3634946 | None:intergenic |
| GTGTACCTCAGCAGCAATTC+AGG | 0.340622 | 5.4:-3630892 | MS.gene024711:CDS |
| AGGAGGCAGGGGATTATTTC+CGG | 0.351958 | 5.4:+3634554 | None:intergenic |
| AATAATTACTTTCAGTCCTT+CGG | 0.352555 | 5.4:+3632703 | None:intergenic |
| GGCTCTCATTGAGGCTGAAC+AGG | 0.356102 | 5.4:-3629132 | MS.gene024711:intron |
| TACAAGCTTCAGATGGTTCA+TGG | 0.356145 | 5.4:-3630779 | MS.gene024711:intron |
| GTCCTGGTGATCTTAGTAAA+TGG | 0.357085 | 5.4:-3632799 | MS.gene024711:CDS |
| AACCTTTGCACATAAATTGC+GGG | 0.357292 | 5.4:+3634195 | None:intergenic |
| GAACAAGAACCTGGATGAAA+TGG | 0.363407 | 5.4:+3634504 | None:intergenic |
| GCTAACCAGGTATGGTTCTT+GGG | 0.364562 | 5.4:-3630589 | MS.gene024711:intron |
| TCCATGATCTTTAGTTGAAA+AGG | 0.374641 | 5.4:+3632748 | None:intergenic |
| TTTCTCAAAGATAATATTGT+TGG | 0.376453 | 5.4:+3634262 | None:intergenic |
| GGTCCTATGTTTCTTATGCC+TGG | 0.385468 | 5.4:-3635360 | MS.gene024711:CDS |
| TCACGTCGGCAGACAACTTT+GGG | 0.389059 | 5.4:-3635661 | MS.gene024711:CDS |
| AAACACTGGGTGGGCTATGC+TGG | 0.390877 | 5.4:-3629153 | MS.gene024711:CDS |
| ACATCTTTCACTCTCACTTT+CGG | 0.391173 | 5.4:+3635486 | None:intergenic |
| TCTTGTGGCGATCCGGAGTT+AGG | 0.392406 | 5.4:+3635630 | None:intergenic |
| ATATCCCCTTCTCCATCGTT+TGG | 0.404510 | 5.4:+3634763 | None:intergenic |
| GAAGGTCCAAGCACCATGTT+TGG | 0.406473 | 5.4:-3634829 | MS.gene024711:CDS |
| CTGTCTAAGCAGCTCCCATC+TGG | 0.406747 | 5.4:-3630462 | MS.gene024711:CDS |
| CAGAATAAGGATGGTGGGTG+GGG | 0.410991 | 5.4:-3634862 | MS.gene024711:CDS |
| ATTCAAATTTGGAAAGCAAC+AGG | 0.422710 | 5.4:-3629187 | MS.gene024711:CDS |
| GAAGATCATCGCTGTGTTTG+TGG | 0.425232 | 5.4:+3635556 | None:intergenic |
| TCGTTACCTTTATAATCATC+AGG | 0.425456 | 5.4:-3634952 | MS.gene024711:intron |
| GTATGAGCCTTTCTTAGAGT+TGG | 0.426533 | 5.4:+3632930 | None:intergenic |
| TATGTTACTCTAAGATTGCT+TGG | 0.426624 | 5.4:-3634793 | MS.gene024711:CDS |
| TATGTTTCTTATGCCTGGCT+TGG | 0.426756 | 5.4:-3635355 | MS.gene024711:intron |
| TTCGGTATCACTTCCTTCAT+CGG | 0.438591 | 5.4:+3635504 | None:intergenic |
| GCACTTGGTTTGGGGTAAAA+GGG | 0.440113 | 5.4:-3630548 | MS.gene024711:CDS |
| GTAAAAGGGCTGATTGCTGC+TGG | 0.442118 | 5.4:-3630534 | MS.gene024711:CDS |
| TGCTAAATATGCTTTGCTGC+TGG | 0.443767 | 5.4:-3633314 | MS.gene024711:CDS |
| TCGGCTGTGCAGTCAGATAT+TGG | 0.447702 | 5.4:+3632722 | None:intergenic |
| ATCCCCTGCCTCCTGAGTTA+TGG | 0.448287 | 5.4:-3634545 | MS.gene024711:CDS |
| AGCAGCTCCCATCTGGTGGT+TGG | 0.448991 | 5.4:-3630455 | MS.gene024711:CDS |
| GTGCTACTCATATAACATCC+TGG | 0.451599 | 5.4:-3634705 | MS.gene024711:CDS |
| TTGTACGGAAAGAGGTTTGT+TGG | 0.453298 | 5.4:-3634295 | MS.gene024711:CDS |
| CAATCACTGCAGAATGAAGA+TGG | 0.453523 | 5.4:-3631736 | MS.gene024711:intron |
| ATCACGTCGGCAGACAACTT+TGG | 0.455540 | 5.4:-3635662 | MS.gene024711:CDS |
| CCATGATGTAGACTGGAATC+AGG | 0.455918 | 5.4:-3634218 | MS.gene024711:CDS |
| TGTCGTTGTTGGTAGGTACT+TGG | 0.456413 | 5.4:-3634591 | MS.gene024711:intron |
| CTTGGAGTGTTTGAATGGTC+CGG | 0.458818 | 5.4:-3634573 | MS.gene024711:CDS |
| AACTATATCCCAGGCATCGT+CGG | 0.461132 | 5.4:-3630851 | MS.gene024711:CDS |
| GGGTGGGGTTTGCATATTGA+AGG | 0.463918 | 5.4:-3634847 | MS.gene024711:CDS |
| CATAGATAATCATGGATCCT+TGG | 0.470111 | 5.4:+3633252 | None:intergenic |
| TTTAACAGATGAGCATAGAA+AGG | 0.472865 | 5.4:-3634982 | MS.gene024711:CDS |
| GGATGAAATGGAAGAATGTA+TGG | 0.473706 | 5.4:+3634516 | None:intergenic |
| ATATGGAGAAGGCACGTGAC+TGG | 0.476887 | 5.4:-3634744 | MS.gene024711:CDS |
| TTCTTTGCTATATAGTTATG+TGG | 0.477165 | 5.4:-3630916 | MS.gene024711:intron |
| GCCAAAAGTTTCAGCAGGAT+TGG | 0.477251 | 5.4:+3631050 | None:intergenic |
| CACTTATGGCACTTGGTTTG+GGG | 0.478518 | 5.4:-3630556 | MS.gene024711:CDS |
| GCTAAATATGCTTTGCTGCT+GGG | 0.479109 | 5.4:-3633313 | MS.gene024711:CDS |
| CCCACCCAGTGTTTACTACA+TGG | 0.481032 | 5.4:+3629162 | None:intergenic |
| AAGATCATCGCTGTGTTTGT+GGG | 0.482356 | 5.4:+3635557 | None:intergenic |
| TTTGCTTCACTTATGGCACT+TGG | 0.482779 | 5.4:-3630563 | MS.gene024711:CDS |
| CCATGATCTTTAGTTGAAAA+GGG | 0.483692 | 5.4:+3632749 | None:intergenic |
| CAGCTCCCATCTGGTGGTTG+GGG | 0.485523 | 5.4:-3630453 | MS.gene024711:CDS |
| ACTCGTGCAAGATATTCTTT+GGG | 0.489176 | 5.4:-3633618 | MS.gene024711:CDS |
| AAAATTGCACCTGAGATTGT+TGG | 0.494234 | 5.4:-3632599 | MS.gene024711:CDS |
| ACTATATCCCAGGCATCGTC+GGG | 0.496045 | 5.4:-3630850 | MS.gene024711:CDS |
| TTGCCAATGTGCTACTTGTA+CGG | 0.496391 | 5.4:-3634310 | MS.gene024711:CDS |
| TGCTACTCATATAACATCCT+GGG | 0.497785 | 5.4:-3634704 | MS.gene024711:CDS |
| GGCGAGATCTTGTGGCGATC+CGG | 0.498854 | 5.4:+3635623 | None:intergenic |
| TGTGCTACTTGTACGGAAAG+AGG | 0.500165 | 5.4:-3634303 | MS.gene024711:CDS |
| TATAATCTCTTTGCATCCAA+TGG | 0.500385 | 5.4:+3632572 | None:intergenic |
| TACATTCTTCCATTTCATCC+AGG | 0.500398 | 5.4:-3634513 | MS.gene024711:intron |
| GGAGCCATAACTCAGGAGGC+AGG | 0.500631 | 5.4:+3634541 | None:intergenic |
| CAGCAATCCATAGATAATCA+TGG | 0.500824 | 5.4:+3633244 | None:intergenic |
| CTTTCAAGTTGCATCTTCCA+AGG | 0.503157 | 5.4:-3633269 | MS.gene024711:CDS |
| AATGGTTCACCAACAATCTC+AGG | 0.503298 | 5.4:+3632590 | None:intergenic |
| ACAGAATAAGGATGGTGGGT+GGG | 0.505290 | 5.4:-3634863 | MS.gene024711:intron |
| TGGTACCGTCACATTTCAAA+AGG | 0.507661 | 5.4:-3632779 | MS.gene024711:CDS |
| AGGTACTTGGAGTGTTTGAA+TGG | 0.508132 | 5.4:-3634578 | MS.gene024711:CDS |
| GTCACATTTCAAAAGGTGCT+TGG | 0.511679 | 5.4:-3632772 | MS.gene024711:CDS |
| CACTCGATCTTATAGCTGGT+TGG | 0.515521 | 5.4:-3631692 | MS.gene024711:CDS |
| TACCATACCATGATGTAGAC+TGG | 0.519792 | 5.4:-3634225 | MS.gene024711:CDS |
| TACATTCATTAAGAATTCAC+AGG | 0.520406 | 5.4:-3632910 | MS.gene024711:intron |
| AGCTCACTCGATCTTATAGC+TGG | 0.520911 | 5.4:-3631696 | MS.gene024711:CDS |
| ATTTCTTCCCGACGATGCCT+GGG | 0.521004 | 5.4:+3630843 | None:intergenic |
| ACAGAACCAAACATGGTGCT+TGG | 0.522416 | 5.4:+3634823 | None:intergenic |
| ATTTGGTCCAACTCTAAGAA+AGG | 0.522891 | 5.4:-3632937 | MS.gene024711:CDS |
| ATGTCGCCAAAAGTTTCAGC+AGG | 0.523669 | 5.4:+3631045 | None:intergenic |
| TCGATATCGGCGAGATCTTG+TGG | 0.524363 | 5.4:+3635615 | None:intergenic |
| AGGGACCAAACGATGGAGAA+GGG | 0.525654 | 5.4:-3634768 | MS.gene024711:CDS |
| TGAGATTGTTGGTGAACCAT+TGG | 0.526436 | 5.4:-3632588 | MS.gene024711:CDS |
| GAGGGACCAAACGATGGAGA+AGG | 0.529699 | 5.4:-3634769 | MS.gene024711:CDS |
| GCTACAATGGAAGTCAGCTA+TGG | 0.532783 | 5.4:-3633010 | MS.gene024711:CDS |
| CATTGGCCTGGTGATTATGC+TGG | 0.535461 | 5.4:-3635381 | MS.gene024711:CDS |
| TCAACTCTTCAGAGTCATGA+TGG | 0.536089 | 5.4:-3635405 | MS.gene024711:CDS |
| CTCTCTCCCCAACCACCAGA+TGG | 0.536827 | 5.4:+3630447 | None:intergenic |
| GTAATTACTCTGTCTATCAC+CGG | 0.537375 | 5.4:-3635020 | MS.gene024711:CDS |
| AAGGTTTCAGAAGATTGTCC+TGG | 0.537463 | 5.4:-3632815 | MS.gene024711:CDS |
| GGCCCGCAATTTATGTGCAA+AGG | 0.538650 | 5.4:-3634197 | MS.gene024711:intron |
| TTGTTTAATGTACGAAGCCA+TGG | 0.540863 | 5.4:+3635684 | None:intergenic |
| AAAGGCTGTCGAGACTGTAA+TGG | 0.544441 | 5.4:-3633534 | MS.gene024711:CDS |
| AATACTCGTTATATTTGCAT+AGG | 0.544620 | 5.4:-3633488 | MS.gene024711:CDS |
| TATGCATTATAGGAAGGATG+TGG | 0.544658 | 5.4:-3634354 | MS.gene024711:intron |
| CTACTAAAACTTACCAAGCC+AGG | 0.544748 | 5.4:+3635342 | None:intergenic |
| GGCTATGCTGGCTCTCATTG+AGG | 0.544878 | 5.4:-3629141 | MS.gene024711:CDS |
| ACATTGGCAAATAGACCATC+CGG | 0.547166 | 5.4:+3634323 | None:intergenic |
| AGCCATAACTCAGGAGGCAG+GGG | 0.548779 | 5.4:+3634543 | None:intergenic |
| AATCACTGCAGAATGAAGAT+GGG | 0.549445 | 5.4:-3631735 | MS.gene024711:intron |
| ATATCTTGCACGAGTGGGTG+AGG | 0.549536 | 5.4:+3633626 | None:intergenic |
| TAAAAGGGCTGATTGCTGCT+GGG | 0.551591 | 5.4:-3630533 | MS.gene024711:CDS |
| GTGTGACAACAACATTAAGA+AGG | 0.552439 | 5.4:-3635443 | MS.gene024711:CDS |
| TTGCAGAAGGAGGCAGTCCA+TGG | 0.553517 | 5.4:-3635701 | MS.gene024711:CDS |
| ACTCTAAGATTGCTTGGTGA+GGG | 0.559954 | 5.4:-3634787 | MS.gene024711:CDS |
| TGCCAAAGAGAATCCGATGA+AGG | 0.562401 | 5.4:-3635517 | MS.gene024711:CDS |
| GAGCCATAACTCAGGAGGCA+GGG | 0.570773 | 5.4:+3634542 | None:intergenic |
| TTCCAGTCTACATCATGGTA+TGG | 0.571064 | 5.4:+3634223 | None:intergenic |
| TAATTACTCTGTCTATCACC+GGG | 0.571843 | 5.4:-3635019 | MS.gene024711:CDS |
| TATGTATTACAGAATAAGGA+TGG | 0.571921 | 5.4:-3634871 | MS.gene024711:intron |
| GAAGGATGTGGTGTCACTGC+CGG | 0.574105 | 5.4:-3634342 | MS.gene024711:CDS |
| TAACCTTTGCACATAAATTG+CGG | 0.575081 | 5.4:+3634194 | None:intergenic |
| TATCTATGGATTGCTGAAGA+TGG | 0.576188 | 5.4:-3633237 | MS.gene024711:CDS |
| TCGATCTTATAGCTGGTTGG+AGG | 0.576532 | 5.4:-3631689 | MS.gene024711:intron |
| AAGCCAGGCATAAGAAACAT+AGG | 0.576662 | 5.4:+3635357 | None:intergenic |
| CTACAATGGAAGTCAGCTAT+GGG | 0.577848 | 5.4:-3633009 | MS.gene024711:CDS |
| GAAATGGAAGAATGTATGGA+AGG | 0.579493 | 5.4:+3634520 | None:intergenic |
| TATTACAGAATAAGGATGGT+GGG | 0.583496 | 5.4:-3634867 | MS.gene024711:intron |
| TTCAGAGTCATGATGGACAT+TGG | 0.583711 | 5.4:-3635398 | MS.gene024711:CDS |
| TACTCTAAGATTGCTTGGTG+AGG | 0.584020 | 5.4:-3634788 | MS.gene024711:CDS |
| TTTCAACTAAAGATCATGGA+TGG | 0.584652 | 5.4:-3632745 | MS.gene024711:CDS |
| CATTTAGGAAACTATATCCC+AGG | 0.586640 | 5.4:-3630860 | MS.gene024711:CDS |
| AGGTCCCATGTAGTAAACAC+TGG | 0.589141 | 5.4:-3629167 | MS.gene024711:CDS |
| CAAAGAATATCTTGCACGAG+TGG | 0.591667 | 5.4:+3633620 | None:intergenic |
| CCTGATTCCAGTCTACATCA+TGG | 0.595918 | 5.4:+3634218 | None:intergenic |
| TCTAAGCAGCTCCCATCTGG+TGG | 0.600699 | 5.4:-3630459 | MS.gene024711:CDS |
| TGGAGAAGGGGATATGGAGA+AGG | 0.601781 | 5.4:-3634755 | MS.gene024711:CDS |
| GTATTACAGAATAAGGATGG+TGG | 0.602509 | 5.4:-3634868 | MS.gene024711:intron |
| TGGGAGTTCGATCCTAACTC+CGG | 0.605594 | 5.4:-3635642 | MS.gene024711:CDS |
| CTAACCAGGTATGGTTCTTG+GGG | 0.606522 | 5.4:-3630588 | MS.gene024711:intron |
| AGTCATGATGGACATTGGCC+TGG | 0.607121 | 5.4:-3635393 | MS.gene024711:CDS |
| TAAAACTGCATTGAGAGCCC+CGG | 0.607279 | 5.4:+3635001 | None:intergenic |
| TCTCTCCCCAACCACCAGAT+GGG | 0.609976 | 5.4:+3630448 | None:intergenic |
| TACAGAATAAGGATGGTGGG+TGG | 0.611556 | 5.4:-3634864 | MS.gene024711:intron |
| ATCACTGCAGAATGAAGATG+GGG | 0.616662 | 5.4:-3631734 | MS.gene024711:intron |
| ACTCAAAACAGAACCAAACA+TGG | 0.622100 | 5.4:+3634816 | None:intergenic |
| GAGTGAGCTCATATGTTGCA+AGG | 0.623263 | 5.4:+3631710 | None:intergenic |
| AAAGAATATCTTGCACGAGT+GGG | 0.627826 | 5.4:+3633621 | None:intergenic |
| CCCATGTAGTAAACACTGGG+TGG | 0.632415 | 5.4:-3629163 | MS.gene024711:CDS |
| TGAAGATGGCATGAAAATGC+AGG | 0.633629 | 5.4:-3633223 | MS.gene024711:intron |
| ATATCTGACTGCACAGCCGA+AGG | 0.633759 | 5.4:-3632719 | MS.gene024711:CDS |
| CGTGACTGGATTCTTGAGCA+TGG | 0.634097 | 5.4:-3634730 | MS.gene024711:CDS |
| CAAAGCCTGAATTGCTGCTG+AGG | 0.637022 | 5.4:+3630887 | None:intergenic |
| CCATGTAGTAAACACTGGGT+GGG | 0.637163 | 5.4:-3629162 | MS.gene024711:CDS |
| TTTCCGTACAAGTAGCACAT+TGG | 0.637997 | 5.4:+3634307 | None:intergenic |
| TGTGACAACAACATTAAGAA+GGG | 0.639121 | 5.4:-3635442 | MS.gene024711:CDS |
| GCTACTCATATAACATCCTG+GGG | 0.640093 | 5.4:-3634703 | MS.gene024711:CDS |
| CTTGGTGAGGGACCAAACGA+TGG | 0.640301 | 5.4:-3634775 | MS.gene024711:CDS |
| GGGACCAAACGATGGAGAAG+GGG | 0.640614 | 5.4:-3634767 | MS.gene024711:CDS |
| TTATCTTTCATGTCAAAACA+AGG | 0.642811 | 5.4:-3630424 | MS.gene024711:intron |
| TCTTTGGGCAACTCTACACA+AGG | 0.644183 | 5.4:-3633603 | MS.gene024711:CDS |
| TACCATTTACTAAGATCACC+AGG | 0.645528 | 5.4:+3632797 | None:intergenic |
| TGGAAACTGAAAGTTGCAGA+AGG | 0.645676 | 5.4:-3635714 | MS.gene024711:CDS |
| CCACCCAGTGTTTACTACAT+GGG | 0.649679 | 5.4:+3629163 | None:intergenic |
| GGTAGAAGATCCAAATTCAG+AGG | 0.657319 | 5.4:-3633292 | MS.gene024711:CDS |
| GGAAGGAGCCATAACTCAGG+AGG | 0.659506 | 5.4:+3634537 | None:intergenic |
| GGTCCCATGTAGTAAACACT+GGG | 0.659996 | 5.4:-3629166 | MS.gene024711:CDS |
| GATGTGGTGTCACTGCCGGA+TGG | 0.661963 | 5.4:-3634338 | MS.gene024711:CDS |
| ATAGGACCAGCATAATCACC+AGG | 0.665129 | 5.4:+3635375 | None:intergenic |
| TATGGAAGGAGCCATAACTC+AGG | 0.668533 | 5.4:+3634534 | None:intergenic |
| AAACTGAAAGTTGCAGAAGG+AGG | 0.670393 | 5.4:-3635711 | MS.gene024711:CDS |
| CTACTCATATAACATCCTGG+GGG | 0.753036 | 5.4:-3634702 | MS.gene024711:CDS |
| GACTGGATTCTTGAGCATGG+TGG | 0.757197 | 5.4:-3634727 | MS.gene024711:CDS |
| TCACTGCAGAATGAAGATGG+GGG | 0.767587 | 5.4:-3631733 | MS.gene024711:intron |
| AATTACTCTGTCTATCACCG+GGG | 0.779289 | 5.4:-3635018 | MS.gene024711:CDS |
| CGTACATTAAACAATCACGT+CGG | 0.788206 | 5.4:-3635675 | MS.gene024711:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 3628380 | 3635739 | 3628380 | ID=MS.gene024711 |
| chr5.4 | mRNA | 3628380 | 3635739 | 3628380 | ID=MS.gene024711.t1;Parent=MS.gene024711 |
| chr5.4 | exon | 3635356 | 3635739 | 3635356 | ID=MS.gene024711.t1.exon1;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3635356 | 3635739 | 3635356 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3634953 | 3635042 | 3634953 | ID=MS.gene024711.t1.exon2;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3634953 | 3635042 | 3634953 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3634687 | 3634881 | 3634687 | ID=MS.gene024711.t1.exon3;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3634687 | 3634881 | 3634687 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3634514 | 3634598 | 3634514 | ID=MS.gene024711.t1.exon4;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3634514 | 3634598 | 3634514 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3634198 | 3634364 | 3634198 | ID=MS.gene024711.t1.exon5;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3634198 | 3634364 | 3634198 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3633475 | 3633663 | 3633475 | ID=MS.gene024711.t1.exon6;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3633475 | 3633663 | 3633475 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3633224 | 3633337 | 3633224 | ID=MS.gene024711.t1.exon7;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3633224 | 3633337 | 3633224 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3632911 | 3633033 | 3632911 | ID=MS.gene024711.t1.exon8;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3632911 | 3633033 | 3632911 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3632712 | 3632834 | 3632712 | ID=MS.gene024711.t1.exon9;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3632712 | 3632834 | 3632712 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3632541 | 3632639 | 3632541 | ID=MS.gene024711.t1.exon10;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3632541 | 3632639 | 3632541 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3631690 | 3631746 | 3631690 | ID=MS.gene024711.t1.exon11;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3631690 | 3631746 | 3631690 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3631030 | 3631076 | 3631030 | ID=MS.gene024711.t1.exon12;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3631030 | 3631076 | 3631030 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3630780 | 3630923 | 3630780 | ID=MS.gene024711.t1.exon13;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3630780 | 3630923 | 3630780 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3630425 | 3630602 | 3630425 | ID=MS.gene024711.t1.exon14;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3630425 | 3630602 | 3630425 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3629133 | 3629213 | 3629133 | ID=MS.gene024711.t1.exon15;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3629133 | 3629213 | 3629133 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| chr5.4 | exon | 3628380 | 3628385 | 3628380 | ID=MS.gene024711.t1.exon16;Parent=MS.gene024711.t1 |
| chr5.4 | CDS | 3628380 | 3628385 | 3628380 | ID=cds.MS.gene024711.t1;Parent=MS.gene024711.t1 |
| Gene Sequence |
| Protein sequence |
