AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025802

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025802.t1 MTR_5g012390 99.714 350 1 0 1 350 1 350 0.0 715
MS.gene025802.t1 MTR_4g101130 86.000 350 49 0 1 350 1 350 0.0 618
MS.gene025802.t1 MTR_4g092690 24.514 257 161 10 39 281 46 283 4.79e-12 66.6
MS.gene025802.t1 MTR_5g014710 24.710 259 160 11 39 282 48 286 7.51e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025802.t1 AT4G17260 77.054 353 78 1 1 350 1 353 0.0 548
MS.gene025802.t1 AT2G22780 24.809 262 162 11 39 285 44 285 1.73e-11 65.1

Find 100 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGTTCAGGAACTAATT+TGG 0.183798 5.3:-5550822 MS.gene025802:CDS
GCTGGGGCTCGCCAGATTAA+TGG 0.260199 5.3:-5551010 MS.gene025802:CDS
GACCTCTGCATCGTCACTGC+TGG 0.269339 5.3:-5551028 MS.gene025802:CDS
TATGAAGTGATCAGTTTAAA+AGG 0.279837 5.3:-5550328 MS.gene025802:CDS
GTTTAAAAGGCTACACTTCT+TGG 0.287297 5.3:-5550315 MS.gene025802:CDS
AGGGTACTCCGTCGCAAATT+TGG 0.306009 5.3:-5550287 MS.gene025802:CDS
TTCCCCTCCAACCGCGTCAT+TGG 0.328564 5.3:-5550839 MS.gene025802:CDS
ACCTCTGCATCGTCACTGCT+GGG 0.330491 5.3:-5551027 MS.gene025802:CDS
CCCTTTGCAAGTACTGAAAC+AGG 0.335982 5.3:+5550229 None:intergenic
ATACACAAAGAGGTTATAAA+TGG 0.340001 5.3:-5550355 MS.gene025802:CDS
CATCAAGATGATCAGCAATA+AGG 0.342388 5.3:+5550780 None:intergenic
TCCAACCGCGTCATTGGTTC+AGG 0.350863 5.3:-5550833 MS.gene025802:CDS
ATCATTGGGACCAGATGGTT+TGG 0.365992 5.3:-5551323 MS.gene025802:CDS
AAAATGCACAAAAGCATATC+AGG 0.366781 5.3:-5551349 MS.gene025802:CDS
CTTGGTAGAGGAGGTGTATT+GGG 0.368641 5.3:-5550157 MS.gene025802:CDS
AAGAGGAAATGAAGAGGCTT+AGG 0.376841 5.3:-5550111 MS.gene025802:CDS
CCTTTGTAGCAAATTCAATC+TGG 0.377803 5.3:+5550982 None:intergenic
ATCTGGACTCGCCATTAATC+TGG 0.381187 5.3:+5550999 None:intergenic
CACAACAAGATCTCAGTTAT+CGG 0.387966 5.3:-5551238 MS.gene025802:CDS
ATCTCGCCTCGTAGCTTGTC+AGG 0.414108 5.3:+5551133 None:intergenic
CTGAATAGTCCGTGGAGGCC+TGG 0.419596 5.3:+5551065 None:intergenic
TTTAAAAGGCTACACTTCTT+GGG 0.420092 5.3:-5550314 MS.gene025802:CDS
TACATTGCTTGGAAACTCTC+TGG 0.433042 5.3:-5550863 MS.gene025802:CDS
AAGCATATCAGGTTCATCAT+TGG 0.435410 5.3:-5551338 MS.gene025802:CDS
CATATTGTAGGCATACATAG+TGG 0.443302 5.3:-5550494 MS.gene025802:intron
ATGTGCTCACTTACATTGCT+TGG 0.446335 5.3:-5550874 MS.gene025802:CDS
AGCATATCAGGTTCATCATT+GGG 0.449605 5.3:-5551337 MS.gene025802:CDS
ATTTGGCTCGAACAATTATG+AGG 0.456393 5.3:-5550270 MS.gene025802:CDS
GCTTGGTAGAGGAGGTGTAT+TGG 0.456494 5.3:-5550158 MS.gene025802:CDS
ATGTAAGTGAGCACATCAAC+AGG 0.458914 5.3:+5550881 None:intergenic
CAAGAACAAGCTCGTCGACT+AGG 0.461803 5.3:+5551167 None:intergenic
TCTTGAGTTGCAGAATCAGT+TGG 0.461924 5.3:-5550071 MS.gene025802:CDS
CGGCGCAGGCAACGTGGGAA+TGG 0.466696 5.3:-5551218 MS.gene025802:CDS
GACTAGGTCTTGTGTTAAGA+TGG 0.470120 5.3:+5551183 None:intergenic
CAAGCTACGAGGCGAGATGC+TGG 0.472234 5.3:-5551128 MS.gene025802:CDS
CTTGAGTTGCAGAATCAGTT+GGG 0.475495 5.3:-5550070 MS.gene025802:CDS
TGAGTTAAGTCCAAACCATC+TGG 0.477368 5.3:+5551313 None:intergenic
TTGTGGCGTTTCGTTGGAGA+AGG 0.483904 5.3:+5551256 None:intergenic
GGAGGCCTGGATCTTAGTGC+GGG 0.491437 5.3:+5551078 None:intergenic
CCCTGTTTCAGTACTTGCAA+AGG 0.493370 5.3:-5550230 MS.gene025802:CDS
GTGACAGTTCAGTGGCACTA+TGG 0.496963 5.3:-5550462 MS.gene025802:CDS
TTGCCTGCACAGCTTGGTAG+AGG 0.498243 5.3:-5550169 MS.gene025802:CDS
CCTCCTCTACCAAGCTGTGC+AGG 0.499160 5.3:+5550166 None:intergenic
GAGAAGGAGGTGAGGCATGT+TGG 0.500290 5.3:+5551272 None:intergenic
GCCTCGTAGCTTGTCAGGAA+TGG 0.502021 5.3:+5551138 None:intergenic
ATCTTGTTGTGGCGTTTCGT+TGG 0.512022 5.3:+5551250 None:intergenic
CCTGTTTCAGTACTTGCAAA+GGG 0.514853 5.3:-5550229 MS.gene025802:CDS
AGAGGAAATGAAGAGGCTTA+GGG 0.518669 5.3:-5550110 MS.gene025802:CDS
CCAGATTGAATTTGCTACAA+AGG 0.522205 5.3:-5550982 MS.gene025802:CDS
GGCTACACTTCTTGGGCCAT+AGG 0.526002 5.3:-5550307 MS.gene025802:CDS
AGTGAGCACATCAACAGGAT+TGG 0.527203 5.3:+5550886 None:intergenic
ATATTGTAGGCATACATAGT+GGG 0.528803 5.3:-5550493 MS.gene025802:intron
AACCAATGACGCGGTTGGAG+GGG 0.528879 5.3:+5550837 None:intergenic
GATGATCAGCAATAAGGAAA+CGG 0.530476 5.3:+5550786 None:intergenic
CCTGCACAGCTTGGTAGAGG+AGG 0.533540 5.3:-5550166 MS.gene025802:CDS
AGGAGGTGAGGCATGTTGGA+TGG 0.541391 5.3:+5551276 None:intergenic
TGGTCAAGCATTAGTGTTGG+AGG 0.542005 5.3:-5550442 MS.gene025802:CDS
ATTAGTTTGCCTGCACAGCT+TGG 0.543386 5.3:-5550175 MS.gene025802:CDS
GAACCAATGACGCGGTTGGA+GGG 0.548021 5.3:+5550836 None:intergenic
GCTACACTTCTTGGGCCATA+GGG 0.551111 5.3:-5550306 MS.gene025802:CDS
GGTTCATCATTGGGACCAGA+TGG 0.553995 5.3:-5551328 MS.gene025802:CDS
TGGCGTTTCGTTGGAGAAGG+AGG 0.556154 5.3:+5551259 None:intergenic
TGAACCAATGACGCGGTTGG+AGG 0.560747 5.3:+5550835 None:intergenic
ATTTGCGACGGAGTACCCTA+TGG 0.561442 5.3:+5550291 None:intergenic
TCCTGAACCAATGACGCGGT+TGG 0.564746 5.3:+5550832 None:intergenic
ATGTTGGATGGACTTGAAGA+AGG 0.565700 5.3:+5551288 None:intergenic
CACTAAGATCCAGGCCTCCA+CGG 0.566976 5.3:-5551074 MS.gene025802:CDS
AAGATCTCAGTTATCGGCGC+AGG 0.570093 5.3:-5551232 MS.gene025802:CDS
GAACAATTATGAGGAATCAA+AGG 0.573740 5.3:-5550261 MS.gene025802:CDS
ACGGACTATTCAGTGACGAT+GGG 0.573834 5.3:-5551055 MS.gene025802:CDS
GCTAGAGAACATACACAAAG+AGG 0.577375 5.3:-5550365 MS.gene025802:CDS
TAATATTCATATGACTGAAG+AGG 0.582508 5.3:-5550128 MS.gene025802:CDS
CTATGGTCAAGCATTAGTGT+TGG 0.582886 5.3:-5550445 MS.gene025802:CDS
TGTTAATGCTCAAGATGTGC+AGG 0.582927 5.3:-5550759 MS.gene025802:intron
CACGGACTATTCAGTGACGA+TGG 0.583882 5.3:-5551056 MS.gene025802:CDS
CCCCAGCAGTGACGATGCAG+AGG 0.586925 5.3:+5551026 None:intergenic
GTTATCGGCGCAGGCAACGT+GGG 0.604202 5.3:-5551223 MS.gene025802:CDS
TGGAGGCCTGGATCTTAGTG+CGG 0.606000 5.3:+5551077 None:intergenic
CCTCTGCATCGTCACTGCTG+GGG 0.608669 5.3:-5551026 MS.gene025802:CDS
TATTGTAGGCATACATAGTG+GGG 0.617018 5.3:-5550492 MS.gene025802:intron
GAGGCCTGGATCTTAGTGCG+GGG 0.617664 5.3:+5551079 None:intergenic
AGTTATCGGCGCAGGCAACG+TGG 0.623394 5.3:-5551224 MS.gene025802:CDS
AGGCCTGGATCTTAGTGCGG+GGG 0.630067 5.3:+5551080 None:intergenic
TGACTGAAGAGGAAATGAAG+AGG 0.635441 5.3:-5550117 MS.gene025802:CDS
GGAACATGGTGACAGTTCAG+TGG 0.640556 5.3:-5550470 MS.gene025802:CDS
ACAATTAGAAGAACACAATG+AGG 0.641975 5.3:+5550911 None:intergenic
TGTTCGAGCCAAATTTGCGA+CGG 0.645980 5.3:+5550279 None:intergenic
TGAGGAGAGTAACGAACCAA+AGG 0.646562 5.3:+5550929 None:intergenic
CGATAACTGAGATCTTGTTG+TGG 0.649606 5.3:+5551239 None:intergenic
GGAGAGTAACGAACCAAAGG+TGG 0.659475 5.3:+5550932 None:intergenic
AATAAGGAAACGGAAGCGAG+AGG 0.662348 5.3:+5550796 None:intergenic
CCTTTGCAAGTACTGAAACA+GGG 0.664039 5.3:+5550230 None:intergenic
CATCGTCACTGAATAGTCCG+TGG 0.664896 5.3:+5551057 None:intergenic
TTTCGTTGGAGAAGGAGGTG+AGG 0.681321 5.3:+5551264 None:intergenic
ATTGTAGGCATACATAGTGG+GGG 0.689959 5.3:-5550491 MS.gene025802:intron
CGGACTATTCAGTGACGATG+GGG 0.708696 5.3:-5551054 MS.gene025802:CDS
GATGCTGGATCTACAGCACG+CGG 0.713694 5.3:-5551113 MS.gene025802:CDS
ACCATTCCTGACAAGCTACG+AGG 0.715464 5.3:-5551139 MS.gene025802:CDS
CGTCACTGAATAGTCCGTGG+AGG 0.718203 5.3:+5551060 None:intergenic
TAGTTCCTGAACCAATGACG+CGG 0.731349 5.3:+5550828 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATTTTTTAAGCAATA+AGG + chr5.3:5550688-5550707 None:intergenic 10.0%
!! TAAAAAAATTATACCACCTT+TGG - chr5.3:5550464-5550483 MS.gene025802:CDS 20.0%
!!! TCAGTTTAATTTCATATTGT+AGG - chr5.3:5550903-5550922 MS.gene025802:CDS 20.0%
! ATACACAAAGAGGTTATAAA+TGG - chr5.3:5551054-5551073 MS.gene025802:CDS 25.0%
! TAATATTCATATGACTGAAG+AGG - chr5.3:5551281-5551300 MS.gene025802:CDS 25.0%
! TATGAAGTGATCAGTTTAAA+AGG - chr5.3:5551081-5551100 MS.gene025802:CDS 25.0%
!!! TAGTGAGTTTGCTAAAATTA+CGG + chr5.3:5550789-5550808 None:intergenic 25.0%
ACAATTAGAAGAACACAATG+AGG + chr5.3:5550501-5550520 None:intergenic 30.0%
ATATTGTAGGCATACATAGT+GGG - chr5.3:5550916-5550935 MS.gene025802:CDS 30.0%
GAACAATTATGAGGAATCAA+AGG - chr5.3:5551148-5551167 MS.gene025802:CDS 30.0%
TTTAAAAGGCTACACTTCTT+GGG - chr5.3:5551095-5551114 MS.gene025802:CDS 30.0%
! TTGCTATTACATTGCATGTT+TGG + chr5.3:5550817-5550836 None:intergenic 30.0%
AAGCATATCAGGTTCATCAT+TGG - chr5.3:5550071-5550090 MS.gene025802:CDS 35.0%
AGCATATCAGGTTCATCATT+GGG - chr5.3:5550072-5550091 MS.gene025802:CDS 35.0%
CACAACAAGATCTCAGTTAT+CGG - chr5.3:5550171-5550190 MS.gene025802:CDS 35.0%
CATATTGTAGGCATACATAG+TGG - chr5.3:5550915-5550934 MS.gene025802:CDS 35.0%
CATCAAGATGATCAGCAATA+AGG + chr5.3:5550632-5550651 None:intergenic 35.0%
CCTTTGTAGCAAATTCAATC+TGG + chr5.3:5550430-5550449 None:intergenic 35.0%
GATGATCAGCAATAAGGAAA+CGG + chr5.3:5550626-5550645 None:intergenic 35.0%
GTTTAAAAGGCTACACTTCT+TGG - chr5.3:5551094-5551113 MS.gene025802:CDS 35.0%
TATTGTAGGCATACATAGTG+GGG - chr5.3:5550917-5550936 MS.gene025802:CDS 35.0%
! ATTTGGCTCGAACAATTATG+AGG - chr5.3:5551139-5551158 MS.gene025802:CDS 35.0%
! GTACTTGCAAAGGGATTTTA+TGG - chr5.3:5551189-5551208 MS.gene025802:CDS 35.0%
! TAAAATTACGGCTGCGATTT+TGG + chr5.3:5550777-5550796 None:intergenic 35.0%
!! AAGGGATTTTATGGCATTGA+TGG - chr5.3:5551198-5551217 MS.gene025802:CDS 35.0%
!! CATTGGTTCAGGAACTAATT+TGG - chr5.3:5550587-5550606 MS.gene025802:intron 35.0%
!! CCAGATTGAATTTGCTACAA+AGG - chr5.3:5550427-5550446 MS.gene025802:CDS 35.0%
AAGAGGAAATGAAGAGGCTT+AGG - chr5.3:5551298-5551317 MS.gene025802:CDS 40.0%
AGAGGAAATGAAGAGGCTTA+GGG - chr5.3:5551299-5551318 MS.gene025802:CDS 40.0%
ATGTAAGTGAGCACATCAAC+AGG + chr5.3:5550531-5550550 None:intergenic 40.0%
ATGTGCTCACTTACATTGCT+TGG - chr5.3:5550535-5550554 MS.gene025802:intron 40.0%
ATTGTAGGCATACATAGTGG+GGG - chr5.3:5550918-5550937 MS.gene025802:CDS 40.0%
CCTGTTTCAGTACTTGCAAA+GGG - chr5.3:5551180-5551199 MS.gene025802:CDS 40.0%
CGATAACTGAGATCTTGTTG+TGG + chr5.3:5550173-5550192 None:intergenic 40.0%
CTATGGTCAAGCATTAGTGT+TGG - chr5.3:5550964-5550983 MS.gene025802:CDS 40.0%
GCTAGAGAACATACACAAAG+AGG - chr5.3:5551044-5551063 MS.gene025802:CDS 40.0%
TACATTGCTTGGAAACTCTC+TGG - chr5.3:5550546-5550565 MS.gene025802:intron 40.0%
TGACTGAAGAGGAAATGAAG+AGG - chr5.3:5551292-5551311 MS.gene025802:CDS 40.0%
TGAGTTAAGTCCAAACCATC+TGG + chr5.3:5550099-5550118 None:intergenic 40.0%
TGTTAATGCTCAAGATGTGC+AGG - chr5.3:5550650-5550669 MS.gene025802:intron 40.0%
TTCTCCAAAAAGCTTAGCAC+TGG + chr5.3:5550997-5551016 None:intergenic 40.0%
! ATGTTGGATGGACTTGAAGA+AGG + chr5.3:5550124-5550143 None:intergenic 40.0%
! CTTGAGTTGCAGAATCAGTT+GGG - chr5.3:5551339-5551358 MS.gene025802:CDS 40.0%
! GACTAGGTCTTGTGTTAAGA+TGG + chr5.3:5550229-5550248 None:intergenic 40.0%
! TCTTGAGTTGCAGAATCAGT+TGG - chr5.3:5551338-5551357 MS.gene025802:CDS 40.0%
! TGTTCCAGTGCTAAGCTTTT+TGG - chr5.3:5550990-5551009 MS.gene025802:CDS 40.0%
!! CCTTTGCAAGTACTGAAACA+GGG + chr5.3:5551182-5551201 None:intergenic 40.0%
!!! GGATTTTATGGCATTGATGG+TGG - chr5.3:5551201-5551220 MS.gene025802:CDS 40.0%
AATAAGGAAACGGAAGCGAG+AGG + chr5.3:5550616-5550635 None:intergenic 45.0%
AGTGAGCACATCAACAGGAT+TGG + chr5.3:5550526-5550545 None:intergenic 45.0%
ATCATTGGGACCAGATGGTT+TGG - chr5.3:5550086-5550105 MS.gene025802:CDS 45.0%
ATCTGGACTCGCCATTAATC+TGG + chr5.3:5550413-5550432 None:intergenic 45.0%
CCCTGTTTCAGTACTTGCAA+AGG - chr5.3:5551179-5551198 MS.gene025802:CDS 45.0%
TAGTTCCTGAACCAATGACG+CGG + chr5.3:5550584-5550603 None:intergenic 45.0%
TGAGGAGAGTAACGAACCAA+AGG + chr5.3:5550483-5550502 None:intergenic 45.0%
TGTTCGAGCCAAATTTGCGA+CGG + chr5.3:5551133-5551152 None:intergenic 45.0%
! ACGGACTATTCAGTGACGAT+GGG - chr5.3:5550354-5550373 MS.gene025802:CDS 45.0%
! ATCTTGTTGTGGCGTTTCGT+TGG + chr5.3:5550162-5550181 None:intergenic 45.0%
! CTTGGTAGAGGAGGTGTATT+GGG - chr5.3:5551252-5551271 MS.gene025802:CDS 45.0%
! TGGTCAAGCATTAGTGTTGG+AGG - chr5.3:5550967-5550986 MS.gene025802:CDS 45.0%
! TTGCGCGCATGTTTGGTAAT+TGG - chr5.3:5550731-5550750 MS.gene025802:intron 45.0%
!! ATTAGTTTGCCTGCACAGCT+TGG - chr5.3:5551234-5551253 MS.gene025802:CDS 45.0%
!! CCCTTTGCAAGTACTGAAAC+AGG + chr5.3:5551183-5551202 None:intergenic 45.0%
AAACATGCGCGCAAGTGCAA+TGG + chr5.3:5550725-5550744 None:intergenic 50.0%
AACATGCGCGCAAGTGCAAT+GGG + chr5.3:5550724-5550743 None:intergenic 50.0%
AAGATCTCAGTTATCGGCGC+AGG - chr5.3:5550177-5550196 MS.gene025802:CDS 50.0%
ACCATTCCTGACAAGCTACG+AGG - chr5.3:5550270-5550289 MS.gene025802:CDS 50.0%
ATTGCACTTGCGCGCATGTT+TGG - chr5.3:5550724-5550743 MS.gene025802:intron 50.0%
CATCGTCACTGAATAGTCCG+TGG + chr5.3:5550355-5550374 None:intergenic 50.0%
CGGACTATTCAGTGACGATG+GGG - chr5.3:5550355-5550374 MS.gene025802:CDS 50.0%
GCTACACTTCTTGGGCCATA+GGG - chr5.3:5551103-5551122 MS.gene025802:CDS 50.0%
GGAACATGGTGACAGTTCAG+TGG - chr5.3:5550939-5550958 MS.gene025802:CDS 50.0%
GGAGAGTAACGAACCAAAGG+TGG + chr5.3:5550480-5550499 None:intergenic 50.0%
GGTTCATCATTGGGACCAGA+TGG - chr5.3:5550081-5550100 MS.gene025802:CDS 50.0%
GTGACAGTTCAGTGGCACTA+TGG - chr5.3:5550947-5550966 MS.gene025802:CDS 50.0%
TTGTGGCGTTTCGTTGGAGA+AGG + chr5.3:5550156-5550175 None:intergenic 50.0%
TTTCGTTGGAGAAGGAGGTG+AGG + chr5.3:5550148-5550167 None:intergenic 50.0%
! AGGGTACTCCGTCGCAAATT+TGG - chr5.3:5551122-5551141 MS.gene025802:CDS 50.0%
! ATTTGCGACGGAGTACCCTA+TGG + chr5.3:5551121-5551140 None:intergenic 50.0%
! CAAGAACAAGCTCGTCGACT+AGG + chr5.3:5550245-5550264 None:intergenic 50.0%
! CACGGACTATTCAGTGACGA+TGG - chr5.3:5550353-5550372 MS.gene025802:CDS 50.0%
! GCATACATAGTGGGGGAACA+TGG - chr5.3:5550925-5550944 MS.gene025802:CDS 50.0%
! GCTTGGTAGAGGAGGTGTAT+TGG - chr5.3:5551251-5551270 MS.gene025802:CDS 50.0%
AACCAATGACGCGGTTGGAG+GGG + chr5.3:5550575-5550594 None:intergenic 55.0%
ACATGCGCGCAAGTGCAATG+GGG + chr5.3:5550723-5550742 None:intergenic 55.0%
ACCTCTGCATCGTCACTGCT+GGG - chr5.3:5550382-5550401 MS.gene025802:CDS 55.0%
AGGAGGTGAGGCATGTTGGA+TGG + chr5.3:5550136-5550155 None:intergenic 55.0%
CACTAAGATCCAGGCCTCCA+CGG - chr5.3:5550335-5550354 MS.gene025802:CDS 55.0%
CGTCACTGAATAGTCCGTGG+AGG + chr5.3:5550352-5550371 None:intergenic 55.0%
GAACCAATGACGCGGTTGGA+GGG + chr5.3:5550576-5550595 None:intergenic 55.0%
GAGAAGGAGGTGAGGCATGT+TGG + chr5.3:5550140-5550159 None:intergenic 55.0%
GATGCTGGATCTACAGCACG+CGG - chr5.3:5550296-5550315 MS.gene025802:CDS 55.0%
GGCTACACTTCTTGGGCCAT+AGG - chr5.3:5551102-5551121 MS.gene025802:CDS 55.0%
TCCTGAACCAATGACGCGGT+TGG + chr5.3:5550580-5550599 None:intergenic 55.0%
TGAACCAATGACGCGGTTGG+AGG + chr5.3:5550577-5550596 None:intergenic 55.0%
TGGAGGCCTGGATCTTAGTG+CGG + chr5.3:5550335-5550354 None:intergenic 55.0%
TGGCGTTTCGTTGGAGAAGG+AGG + chr5.3:5550153-5550172 None:intergenic 55.0%
TTGCCTGCACAGCTTGGTAG+AGG - chr5.3:5551240-5551259 MS.gene025802:CDS 55.0%
! ATCTCGCCTCGTAGCTTGTC+AGG + chr5.3:5550279-5550298 None:intergenic 55.0%
! GCCTCGTAGCTTGTCAGGAA+TGG + chr5.3:5550274-5550293 None:intergenic 55.0%
!! TCCAACCGCGTCATTGGTTC+AGG - chr5.3:5550576-5550595 MS.gene025802:intron 55.0%
AGGCCTGGATCTTAGTGCGG+GGG + chr5.3:5550332-5550351 None:intergenic 60.0%
AGTTATCGGCGCAGGCAACG+TGG - chr5.3:5550185-5550204 MS.gene025802:CDS 60.0%
CAAGCTACGAGGCGAGATGC+TGG - chr5.3:5550281-5550300 MS.gene025802:CDS 60.0%
CCTCCTCTACCAAGCTGTGC+AGG + chr5.3:5551246-5551265 None:intergenic 60.0%
CCTCTGCATCGTCACTGCTG+GGG - chr5.3:5550383-5550402 MS.gene025802:CDS 60.0%
CCTGCACAGCTTGGTAGAGG+AGG - chr5.3:5551243-5551262 MS.gene025802:CDS 60.0%
CTGAATAGTCCGTGGAGGCC+TGG + chr5.3:5550347-5550366 None:intergenic 60.0%
GACCTCTGCATCGTCACTGC+TGG - chr5.3:5550381-5550400 MS.gene025802:CDS 60.0%
GAGGCCTGGATCTTAGTGCG+GGG + chr5.3:5550333-5550352 None:intergenic 60.0%
GATCTTAGTGCGGGGGAGGA+AGG + chr5.3:5550325-5550344 None:intergenic 60.0%
GCTGGGGCTCGCCAGATTAA+TGG - chr5.3:5550399-5550418 MS.gene025802:CDS 60.0%
GGAGGCCTGGATCTTAGTGC+GGG + chr5.3:5550334-5550353 None:intergenic 60.0%
GTTATCGGCGCAGGCAACGT+GGG - chr5.3:5550186-5550205 MS.gene025802:CDS 60.0%
TTCCCCTCCAACCGCGTCAT+TGG - chr5.3:5550570-5550589 MS.gene025802:intron 60.0%
CCCCAGCAGTGACGATGCAG+AGG + chr5.3:5550386-5550405 None:intergenic 65.0%
CCTCCCCCGCACTAAGATCC+AGG - chr5.3:5550326-5550345 MS.gene025802:CDS 65.0%
CCTGGATCTTAGTGCGGGGG+AGG + chr5.3:5550329-5550348 None:intergenic 65.0%
CGGCGCAGGCAACGTGGGAA+TGG - chr5.3:5550191-5550210 MS.gene025802:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 5550063 5551368 5550063 ID=MS.gene025802
chr5.3 mRNA 5550063 5551368 5550063 ID=MS.gene025802.t1;Parent=MS.gene025802
chr5.3 exon 5550760 5551368 5550760 ID=MS.gene025802.t1.exon1;Parent=MS.gene025802.t1
chr5.3 CDS 5550760 5551368 5550760 ID=cds.MS.gene025802.t1;Parent=MS.gene025802.t1
chr5.3 exon 5550063 5550506 5550063 ID=MS.gene025802.t1.exon2;Parent=MS.gene025802.t1
chr5.3 CDS 5550063 5550506 5550063 ID=cds.MS.gene025802.t1;Parent=MS.gene025802.t1
Gene Sequence

>MS.gene025802

ATGCACAAAAGCATATCAGGTTCATCATTGGGACCAGATGGTTTGGACTTAACTCAGTCCTTCTTCAAGTCCATCCAACATGCCTCACCTCCTTCTCCAACGAAACGCCACAACAAGATCTCAGTTATCGGCGCAGGCAACGTGGGAATGGCTATAGCTCAAACCATCTTAACACAAGACCTAGTCGACGAGCTTGTTCTTGTCGACACCATTCCTGACAAGCTACGAGGCGAGATGCTGGATCTACAGCACGCGGCAGCCTTCCTCCCCCGCACTAAGATCCAGGCCTCCACGGACTATTCAGTGACGATGGGGTCTGACCTCTGCATCGTCACTGCTGGGGCTCGCCAGATTAATGGCGAGTCCAGATTGAATTTGCTACAAAGGAATGTTGCTTTGTTTAAAAAAATTATACCACCTTTGGTTCGTTACTCTCCTCATTGTGTTCTTCTAATTGTTTCCAATCCTGTTGATGTGCTCACTTACATTGCTTGGAAACTCTCTGGTTTCCCCTCCAACCGCGTCATTGGTTCAGGAACTAATTTGGACTCCTCTCGCTTCCGTTTCCTTATTGCTGATCATCTTGATGTTAATGCTCAAGATGTGCAGGTACAACTTTTTTCCTTATTGCTTAAAAAATATATATCGTCGATCATTCCCCATTGCACTTGCGCGCATGTTTGGTAATTGGTATCACAGTGAATAGTTTTTCCAAAATCGCAGCCGTAATTTTAGCAAACTCACTATAATACCAAACATGCAATGTAATAGCAATTGCAAAACGTAATTTAGAACTAACACTGATCACGAACGAACACACATAGTAAACTCAATATTTTATCAGTTTAATTTCATATTGTAGGCATACATAGTGGGGGAACATGGTGACAGTTCAGTGGCACTATGGTCAAGCATTAGTGTTGGAGGTGTTCCAGTGCTAAGCTTTTTGGAGAAACAACAAATAGCTTACGAGAAAGAAATGCTAGAGAACATACACAAAGAGGTTATAAATGGCGCATATGAAGTGATCAGTTTAAAAGGCTACACTTCTTGGGCCATAGGGTACTCCGTCGCAAATTTGGCTCGAACAATTATGAGGAATCAAAGGAAGATTCACCCTGTTTCAGTACTTGCAAAGGGATTTTATGGCATTGATGGTGGAGAAGTGTTTATTAGTTTGCCTGCACAGCTTGGTAGAGGAGGTGTATTGGGTGTGACTAATATTCATATGACTGAAGAGGAAATGAAGAGGCTTAGGGACTCTGCTATCACTATTCTTGAGTTGCAGAATCAGTTGGGTATTTGA
Protein sequence

>MS.gene025802.t1

MHKSISGSSLGPDGLDLTQSFFKSIQHASPPSPTKRHNKISVIGAGNVGMAIAQTILTQDLVDELVLVDTIPDKLRGEMLDLQHAAAFLPRTKIQASTDYSVTMGSDLCIVTAGARQINGESRLNLLQRNVALFKKIIPPLVRYSPHCVLLIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQQIAYEKEMLENIHKEVINGAYEVISLKGYTSWAIGYSVANLARTIMRNQRKIHPVSVLAKGFYGIDGGEVFISLPAQLGRGGVLGVTNIHMTEEEMKRLRDSAITILELQNQLGI