AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene025803

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025803.t1 MTR_5g012390 99.714 350 1 0 1 350 1 350 0.0 715
MS.gene025803.t1 MTR_4g101130 86.000 350 49 0 1 350 1 350 0.0 618
MS.gene025803.t1 MTR_4g092690 24.514 257 161 10 39 281 46 283 4.79e-12 66.6
MS.gene025803.t1 MTR_5g014710 24.710 259 160 11 39 282 48 286 7.51e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene025803.t1 AT4G17260 77.054 353 78 1 1 350 1 353 0.0 548
MS.gene025803.t1 AT2G22780 24.809 262 162 11 39 285 44 285 1.73e-11 65.1

Find 100 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTGGTTCAGGAACTAATT+TGG 0.183798 5.3:-5574867 MS.gene025803:CDS
GCTGGGGCTCGCCAGATTAA+TGG 0.260199 5.3:-5575055 MS.gene025803:CDS
GACCTCTGCATCGTCACTGC+TGG 0.269339 5.3:-5575073 MS.gene025803:CDS
TATGAAGTGATCAGTTTAAA+AGG 0.279837 5.3:-5574373 MS.gene025803:CDS
GTTTAAAAGGCTACACTTCT+TGG 0.287297 5.3:-5574360 MS.gene025803:CDS
AGGGTACTCCGTCGCAAATT+TGG 0.306009 5.3:-5574332 MS.gene025803:CDS
TTCCCCTCCAACCGCGTCAT+TGG 0.328564 5.3:-5574884 MS.gene025803:CDS
ACCTCTGCATCGTCACTGCT+GGG 0.330491 5.3:-5575072 MS.gene025803:CDS
CCCTTTGCAAGTACTGAAAC+AGG 0.335982 5.3:+5574274 None:intergenic
ATACACAAAGAGGTTATAAA+TGG 0.340001 5.3:-5574400 MS.gene025803:CDS
CATCAAGATGATCAGCAATA+AGG 0.342388 5.3:+5574825 None:intergenic
TCCAACCGCGTCATTGGTTC+AGG 0.350863 5.3:-5574878 MS.gene025803:CDS
ATCATTGGGACCAGATGGTT+TGG 0.365992 5.3:-5575368 MS.gene025803:CDS
AAAATGCACAAAAGCATATC+AGG 0.366781 5.3:-5575394 MS.gene025803:CDS
CTTGGTAGAGGAGGTGTATT+GGG 0.368641 5.3:-5574202 MS.gene025803:CDS
AAGAGGAAATGAAGAGGCTT+AGG 0.376841 5.3:-5574156 MS.gene025803:CDS
CCTTTGTAGCAAATTCAATC+TGG 0.377803 5.3:+5575027 None:intergenic
ATCTGGACTCGCCATTAATC+TGG 0.381187 5.3:+5575044 None:intergenic
CACAACAAGATCTCAGTTAT+CGG 0.387966 5.3:-5575283 MS.gene025803:CDS
ATCTCGCCTCGTAGCTTGTC+AGG 0.414108 5.3:+5575178 None:intergenic
CTGAATAGTCCGTGGAGGCC+TGG 0.419596 5.3:+5575110 None:intergenic
TTTAAAAGGCTACACTTCTT+GGG 0.420092 5.3:-5574359 MS.gene025803:CDS
TACATTGCTTGGAAACTCTC+TGG 0.433042 5.3:-5574908 MS.gene025803:CDS
AAGCATATCAGGTTCATCAT+TGG 0.435410 5.3:-5575383 MS.gene025803:CDS
CATATTGTAGGCATACATAG+TGG 0.443302 5.3:-5574539 MS.gene025803:intron
ATGTGCTCACTTACATTGCT+TGG 0.446335 5.3:-5574919 MS.gene025803:CDS
AGCATATCAGGTTCATCATT+GGG 0.449605 5.3:-5575382 MS.gene025803:CDS
ATTTGGCTCGAACAATTATG+AGG 0.456393 5.3:-5574315 MS.gene025803:CDS
GCTTGGTAGAGGAGGTGTAT+TGG 0.456494 5.3:-5574203 MS.gene025803:CDS
ATGTAAGTGAGCACATCAAC+AGG 0.458914 5.3:+5574926 None:intergenic
CAAGAACAAGCTCGTCGACT+AGG 0.461803 5.3:+5575212 None:intergenic
TCTTGAGTTGCAGAATCAGT+TGG 0.461924 5.3:-5574116 MS.gene025803:CDS
CGGCGCAGGCAACGTGGGAA+TGG 0.466696 5.3:-5575263 MS.gene025803:CDS
GACTAGGTCTTGTGTTAAGA+TGG 0.470120 5.3:+5575228 None:intergenic
CAAGCTACGAGGCGAGATGC+TGG 0.472234 5.3:-5575173 MS.gene025803:CDS
CTTGAGTTGCAGAATCAGTT+GGG 0.475495 5.3:-5574115 MS.gene025803:CDS
TGAGTTAAGTCCAAACCATC+TGG 0.477368 5.3:+5575358 None:intergenic
TTGTGGCGTTTCGTTGGAGA+AGG 0.483904 5.3:+5575301 None:intergenic
GGAGGCCTGGATCTTAGTGC+GGG 0.491437 5.3:+5575123 None:intergenic
CCCTGTTTCAGTACTTGCAA+AGG 0.493370 5.3:-5574275 MS.gene025803:CDS
GTGACAGTTCAGTGGCACTA+TGG 0.496963 5.3:-5574507 MS.gene025803:CDS
TTGCCTGCACAGCTTGGTAG+AGG 0.498243 5.3:-5574214 MS.gene025803:CDS
CCTCCTCTACCAAGCTGTGC+AGG 0.499160 5.3:+5574211 None:intergenic
GAGAAGGAGGTGAGGCATGT+TGG 0.500290 5.3:+5575317 None:intergenic
GCCTCGTAGCTTGTCAGGAA+TGG 0.502021 5.3:+5575183 None:intergenic
ATCTTGTTGTGGCGTTTCGT+TGG 0.512022 5.3:+5575295 None:intergenic
CCTGTTTCAGTACTTGCAAA+GGG 0.514853 5.3:-5574274 MS.gene025803:CDS
AGAGGAAATGAAGAGGCTTA+GGG 0.518669 5.3:-5574155 MS.gene025803:CDS
CCAGATTGAATTTGCTACAA+AGG 0.522205 5.3:-5575027 MS.gene025803:CDS
GGCTACACTTCTTGGGCCAT+AGG 0.526002 5.3:-5574352 MS.gene025803:CDS
AGTGAGCACATCAACAGGAT+TGG 0.527203 5.3:+5574931 None:intergenic
ATATTGTAGGCATACATAGT+GGG 0.528803 5.3:-5574538 MS.gene025803:intron
AACCAATGACGCGGTTGGAG+GGG 0.528879 5.3:+5574882 None:intergenic
GATGATCAGCAATAAGGAAA+CGG 0.530476 5.3:+5574831 None:intergenic
CCTGCACAGCTTGGTAGAGG+AGG 0.533540 5.3:-5574211 MS.gene025803:CDS
AGGAGGTGAGGCATGTTGGA+TGG 0.541391 5.3:+5575321 None:intergenic
TGGTCAAGCATTAGTGTTGG+AGG 0.542005 5.3:-5574487 MS.gene025803:CDS
ATTAGTTTGCCTGCACAGCT+TGG 0.543386 5.3:-5574220 MS.gene025803:CDS
GAACCAATGACGCGGTTGGA+GGG 0.548021 5.3:+5574881 None:intergenic
GCTACACTTCTTGGGCCATA+GGG 0.551111 5.3:-5574351 MS.gene025803:CDS
GGTTCATCATTGGGACCAGA+TGG 0.553995 5.3:-5575373 MS.gene025803:CDS
TGGCGTTTCGTTGGAGAAGG+AGG 0.556154 5.3:+5575304 None:intergenic
TGAACCAATGACGCGGTTGG+AGG 0.560747 5.3:+5574880 None:intergenic
ATTTGCGACGGAGTACCCTA+TGG 0.561442 5.3:+5574336 None:intergenic
TCCTGAACCAATGACGCGGT+TGG 0.564746 5.3:+5574877 None:intergenic
ATGTTGGATGGACTTGAAGA+AGG 0.565700 5.3:+5575333 None:intergenic
CACTAAGATCCAGGCCTCCA+CGG 0.566976 5.3:-5575119 MS.gene025803:CDS
AAGATCTCAGTTATCGGCGC+AGG 0.570093 5.3:-5575277 MS.gene025803:CDS
GAACAATTATGAGGAATCAA+AGG 0.573740 5.3:-5574306 MS.gene025803:CDS
ACGGACTATTCAGTGACGAT+GGG 0.573834 5.3:-5575100 MS.gene025803:CDS
GCTAGAGAACATACACAAAG+AGG 0.577375 5.3:-5574410 MS.gene025803:CDS
TAATATTCATATGACTGAAG+AGG 0.582508 5.3:-5574173 MS.gene025803:CDS
CTATGGTCAAGCATTAGTGT+TGG 0.582886 5.3:-5574490 MS.gene025803:CDS
TGTTAATGCTCAAGATGTGC+AGG 0.582927 5.3:-5574804 MS.gene025803:intron
CACGGACTATTCAGTGACGA+TGG 0.583882 5.3:-5575101 MS.gene025803:CDS
CCCCAGCAGTGACGATGCAG+AGG 0.586925 5.3:+5575071 None:intergenic
GTTATCGGCGCAGGCAACGT+GGG 0.604202 5.3:-5575268 MS.gene025803:CDS
TGGAGGCCTGGATCTTAGTG+CGG 0.606000 5.3:+5575122 None:intergenic
CCTCTGCATCGTCACTGCTG+GGG 0.608669 5.3:-5575071 MS.gene025803:CDS
TATTGTAGGCATACATAGTG+GGG 0.617018 5.3:-5574537 MS.gene025803:intron
GAGGCCTGGATCTTAGTGCG+GGG 0.617664 5.3:+5575124 None:intergenic
AGTTATCGGCGCAGGCAACG+TGG 0.623394 5.3:-5575269 MS.gene025803:CDS
AGGCCTGGATCTTAGTGCGG+GGG 0.630067 5.3:+5575125 None:intergenic
TGACTGAAGAGGAAATGAAG+AGG 0.635441 5.3:-5574162 MS.gene025803:CDS
GGAACATGGTGACAGTTCAG+TGG 0.640556 5.3:-5574515 MS.gene025803:CDS
ACAATTAGAAGAACACAATG+AGG 0.641975 5.3:+5574956 None:intergenic
TGTTCGAGCCAAATTTGCGA+CGG 0.645980 5.3:+5574324 None:intergenic
TGAGGAGAGTAACGAACCAA+AGG 0.646562 5.3:+5574974 None:intergenic
CGATAACTGAGATCTTGTTG+TGG 0.649606 5.3:+5575284 None:intergenic
GGAGAGTAACGAACCAAAGG+TGG 0.659475 5.3:+5574977 None:intergenic
AATAAGGAAACGGAAGCGAG+AGG 0.662348 5.3:+5574841 None:intergenic
CCTTTGCAAGTACTGAAACA+GGG 0.664039 5.3:+5574275 None:intergenic
CATCGTCACTGAATAGTCCG+TGG 0.664896 5.3:+5575102 None:intergenic
TTTCGTTGGAGAAGGAGGTG+AGG 0.681321 5.3:+5575309 None:intergenic
ATTGTAGGCATACATAGTGG+GGG 0.689959 5.3:-5574536 MS.gene025803:intron
CGGACTATTCAGTGACGATG+GGG 0.708696 5.3:-5575099 MS.gene025803:CDS
GATGCTGGATCTACAGCACG+CGG 0.713694 5.3:-5575158 MS.gene025803:CDS
ACCATTCCTGACAAGCTACG+AGG 0.715464 5.3:-5575184 MS.gene025803:CDS
CGTCACTGAATAGTCCGTGG+AGG 0.718203 5.3:+5575105 None:intergenic
TAGTTCCTGAACCAATGACG+CGG 0.731349 5.3:+5574873 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATATATTTTTTAAGCAATA+AGG + chr5.3:5574733-5574752 None:intergenic 10.0%
!! TAAAAAAATTATACCACCTT+TGG - chr5.3:5574509-5574528 MS.gene025803:CDS 20.0%
!!! TCAGTTTAATTTCATATTGT+AGG - chr5.3:5574948-5574967 MS.gene025803:CDS 20.0%
! ATACACAAAGAGGTTATAAA+TGG - chr5.3:5575099-5575118 MS.gene025803:CDS 25.0%
! TAATATTCATATGACTGAAG+AGG - chr5.3:5575326-5575345 MS.gene025803:CDS 25.0%
! TATGAAGTGATCAGTTTAAA+AGG - chr5.3:5575126-5575145 MS.gene025803:CDS 25.0%
!!! TAGTGAGTTTGCTAAAATTA+CGG + chr5.3:5574834-5574853 None:intergenic 25.0%
ACAATTAGAAGAACACAATG+AGG + chr5.3:5574546-5574565 None:intergenic 30.0%
ATATTGTAGGCATACATAGT+GGG - chr5.3:5574961-5574980 MS.gene025803:CDS 30.0%
GAACAATTATGAGGAATCAA+AGG - chr5.3:5575193-5575212 MS.gene025803:CDS 30.0%
TTTAAAAGGCTACACTTCTT+GGG - chr5.3:5575140-5575159 MS.gene025803:CDS 30.0%
! TTGCTATTACATTGCATGTT+TGG + chr5.3:5574862-5574881 None:intergenic 30.0%
AAGCATATCAGGTTCATCAT+TGG - chr5.3:5574116-5574135 MS.gene025803:CDS 35.0%
AGCATATCAGGTTCATCATT+GGG - chr5.3:5574117-5574136 MS.gene025803:CDS 35.0%
CACAACAAGATCTCAGTTAT+CGG - chr5.3:5574216-5574235 MS.gene025803:CDS 35.0%
CATATTGTAGGCATACATAG+TGG - chr5.3:5574960-5574979 MS.gene025803:CDS 35.0%
CATCAAGATGATCAGCAATA+AGG + chr5.3:5574677-5574696 None:intergenic 35.0%
CCTTTGTAGCAAATTCAATC+TGG + chr5.3:5574475-5574494 None:intergenic 35.0%
GATGATCAGCAATAAGGAAA+CGG + chr5.3:5574671-5574690 None:intergenic 35.0%
GTTTAAAAGGCTACACTTCT+TGG - chr5.3:5575139-5575158 MS.gene025803:CDS 35.0%
TATTGTAGGCATACATAGTG+GGG - chr5.3:5574962-5574981 MS.gene025803:CDS 35.0%
! ATTTGGCTCGAACAATTATG+AGG - chr5.3:5575184-5575203 MS.gene025803:CDS 35.0%
! GTACTTGCAAAGGGATTTTA+TGG - chr5.3:5575234-5575253 MS.gene025803:CDS 35.0%
! TAAAATTACGGCTGCGATTT+TGG + chr5.3:5574822-5574841 None:intergenic 35.0%
!! AAGGGATTTTATGGCATTGA+TGG - chr5.3:5575243-5575262 MS.gene025803:CDS 35.0%
!! CATTGGTTCAGGAACTAATT+TGG - chr5.3:5574632-5574651 MS.gene025803:intron 35.0%
!! CCAGATTGAATTTGCTACAA+AGG - chr5.3:5574472-5574491 MS.gene025803:CDS 35.0%
AAGAGGAAATGAAGAGGCTT+AGG - chr5.3:5575343-5575362 MS.gene025803:CDS 40.0%
AGAGGAAATGAAGAGGCTTA+GGG - chr5.3:5575344-5575363 MS.gene025803:CDS 40.0%
ATGTAAGTGAGCACATCAAC+AGG + chr5.3:5574576-5574595 None:intergenic 40.0%
ATGTGCTCACTTACATTGCT+TGG - chr5.3:5574580-5574599 MS.gene025803:intron 40.0%
ATTGTAGGCATACATAGTGG+GGG - chr5.3:5574963-5574982 MS.gene025803:CDS 40.0%
CCTGTTTCAGTACTTGCAAA+GGG - chr5.3:5575225-5575244 MS.gene025803:CDS 40.0%
CGATAACTGAGATCTTGTTG+TGG + chr5.3:5574218-5574237 None:intergenic 40.0%
CTATGGTCAAGCATTAGTGT+TGG - chr5.3:5575009-5575028 MS.gene025803:CDS 40.0%
GCTAGAGAACATACACAAAG+AGG - chr5.3:5575089-5575108 MS.gene025803:CDS 40.0%
TACATTGCTTGGAAACTCTC+TGG - chr5.3:5574591-5574610 MS.gene025803:intron 40.0%
TGACTGAAGAGGAAATGAAG+AGG - chr5.3:5575337-5575356 MS.gene025803:CDS 40.0%
TGAGTTAAGTCCAAACCATC+TGG + chr5.3:5574144-5574163 None:intergenic 40.0%
TGTTAATGCTCAAGATGTGC+AGG - chr5.3:5574695-5574714 MS.gene025803:intron 40.0%
TTCTCCAAAAAGCTTAGCAC+TGG + chr5.3:5575042-5575061 None:intergenic 40.0%
! ATGTTGGATGGACTTGAAGA+AGG + chr5.3:5574169-5574188 None:intergenic 40.0%
! CTTGAGTTGCAGAATCAGTT+GGG - chr5.3:5575384-5575403 MS.gene025803:CDS 40.0%
! GACTAGGTCTTGTGTTAAGA+TGG + chr5.3:5574274-5574293 None:intergenic 40.0%
! TCTTGAGTTGCAGAATCAGT+TGG - chr5.3:5575383-5575402 MS.gene025803:CDS 40.0%
! TGTTCCAGTGCTAAGCTTTT+TGG - chr5.3:5575035-5575054 MS.gene025803:CDS 40.0%
!! CCTTTGCAAGTACTGAAACA+GGG + chr5.3:5575227-5575246 None:intergenic 40.0%
!!! GGATTTTATGGCATTGATGG+TGG - chr5.3:5575246-5575265 MS.gene025803:CDS 40.0%
AATAAGGAAACGGAAGCGAG+AGG + chr5.3:5574661-5574680 None:intergenic 45.0%
AGTGAGCACATCAACAGGAT+TGG + chr5.3:5574571-5574590 None:intergenic 45.0%
ATCATTGGGACCAGATGGTT+TGG - chr5.3:5574131-5574150 MS.gene025803:CDS 45.0%
ATCTGGACTCGCCATTAATC+TGG + chr5.3:5574458-5574477 None:intergenic 45.0%
CCCTGTTTCAGTACTTGCAA+AGG - chr5.3:5575224-5575243 MS.gene025803:CDS 45.0%
TAGTTCCTGAACCAATGACG+CGG + chr5.3:5574629-5574648 None:intergenic 45.0%
TGAGGAGAGTAACGAACCAA+AGG + chr5.3:5574528-5574547 None:intergenic 45.0%
TGTTCGAGCCAAATTTGCGA+CGG + chr5.3:5575178-5575197 None:intergenic 45.0%
! ACGGACTATTCAGTGACGAT+GGG - chr5.3:5574399-5574418 MS.gene025803:CDS 45.0%
! ATCTTGTTGTGGCGTTTCGT+TGG + chr5.3:5574207-5574226 None:intergenic 45.0%
! CTTGGTAGAGGAGGTGTATT+GGG - chr5.3:5575297-5575316 MS.gene025803:CDS 45.0%
! TGGTCAAGCATTAGTGTTGG+AGG - chr5.3:5575012-5575031 MS.gene025803:CDS 45.0%
! TTGCGCGCATGTTTGGTAAT+TGG - chr5.3:5574776-5574795 MS.gene025803:intron 45.0%
!! ATTAGTTTGCCTGCACAGCT+TGG - chr5.3:5575279-5575298 MS.gene025803:CDS 45.0%
!! CCCTTTGCAAGTACTGAAAC+AGG + chr5.3:5575228-5575247 None:intergenic 45.0%
AAACATGCGCGCAAGTGCAA+TGG + chr5.3:5574770-5574789 None:intergenic 50.0%
AACATGCGCGCAAGTGCAAT+GGG + chr5.3:5574769-5574788 None:intergenic 50.0%
AAGATCTCAGTTATCGGCGC+AGG - chr5.3:5574222-5574241 MS.gene025803:CDS 50.0%
ACCATTCCTGACAAGCTACG+AGG - chr5.3:5574315-5574334 MS.gene025803:CDS 50.0%
ATTGCACTTGCGCGCATGTT+TGG - chr5.3:5574769-5574788 MS.gene025803:intron 50.0%
CATCGTCACTGAATAGTCCG+TGG + chr5.3:5574400-5574419 None:intergenic 50.0%
CGGACTATTCAGTGACGATG+GGG - chr5.3:5574400-5574419 MS.gene025803:CDS 50.0%
GCTACACTTCTTGGGCCATA+GGG - chr5.3:5575148-5575167 MS.gene025803:CDS 50.0%
GGAACATGGTGACAGTTCAG+TGG - chr5.3:5574984-5575003 MS.gene025803:CDS 50.0%
GGAGAGTAACGAACCAAAGG+TGG + chr5.3:5574525-5574544 None:intergenic 50.0%
GGTTCATCATTGGGACCAGA+TGG - chr5.3:5574126-5574145 MS.gene025803:CDS 50.0%
GTGACAGTTCAGTGGCACTA+TGG - chr5.3:5574992-5575011 MS.gene025803:CDS 50.0%
TTGTGGCGTTTCGTTGGAGA+AGG + chr5.3:5574201-5574220 None:intergenic 50.0%
TTTCGTTGGAGAAGGAGGTG+AGG + chr5.3:5574193-5574212 None:intergenic 50.0%
! AGGGTACTCCGTCGCAAATT+TGG - chr5.3:5575167-5575186 MS.gene025803:CDS 50.0%
! ATTTGCGACGGAGTACCCTA+TGG + chr5.3:5575166-5575185 None:intergenic 50.0%
! CAAGAACAAGCTCGTCGACT+AGG + chr5.3:5574290-5574309 None:intergenic 50.0%
! CACGGACTATTCAGTGACGA+TGG - chr5.3:5574398-5574417 MS.gene025803:CDS 50.0%
! GCATACATAGTGGGGGAACA+TGG - chr5.3:5574970-5574989 MS.gene025803:CDS 50.0%
! GCTTGGTAGAGGAGGTGTAT+TGG - chr5.3:5575296-5575315 MS.gene025803:CDS 50.0%
AACCAATGACGCGGTTGGAG+GGG + chr5.3:5574620-5574639 None:intergenic 55.0%
ACATGCGCGCAAGTGCAATG+GGG + chr5.3:5574768-5574787 None:intergenic 55.0%
ACCTCTGCATCGTCACTGCT+GGG - chr5.3:5574427-5574446 MS.gene025803:CDS 55.0%
AGGAGGTGAGGCATGTTGGA+TGG + chr5.3:5574181-5574200 None:intergenic 55.0%
CACTAAGATCCAGGCCTCCA+CGG - chr5.3:5574380-5574399 MS.gene025803:CDS 55.0%
CGTCACTGAATAGTCCGTGG+AGG + chr5.3:5574397-5574416 None:intergenic 55.0%
GAACCAATGACGCGGTTGGA+GGG + chr5.3:5574621-5574640 None:intergenic 55.0%
GAGAAGGAGGTGAGGCATGT+TGG + chr5.3:5574185-5574204 None:intergenic 55.0%
GATGCTGGATCTACAGCACG+CGG - chr5.3:5574341-5574360 MS.gene025803:CDS 55.0%
GGCTACACTTCTTGGGCCAT+AGG - chr5.3:5575147-5575166 MS.gene025803:CDS 55.0%
TCCTGAACCAATGACGCGGT+TGG + chr5.3:5574625-5574644 None:intergenic 55.0%
TGAACCAATGACGCGGTTGG+AGG + chr5.3:5574622-5574641 None:intergenic 55.0%
TGGAGGCCTGGATCTTAGTG+CGG + chr5.3:5574380-5574399 None:intergenic 55.0%
TGGCGTTTCGTTGGAGAAGG+AGG + chr5.3:5574198-5574217 None:intergenic 55.0%
TTGCCTGCACAGCTTGGTAG+AGG - chr5.3:5575285-5575304 MS.gene025803:CDS 55.0%
! ATCTCGCCTCGTAGCTTGTC+AGG + chr5.3:5574324-5574343 None:intergenic 55.0%
! GCCTCGTAGCTTGTCAGGAA+TGG + chr5.3:5574319-5574338 None:intergenic 55.0%
!! TCCAACCGCGTCATTGGTTC+AGG - chr5.3:5574621-5574640 MS.gene025803:intron 55.0%
AGGCCTGGATCTTAGTGCGG+GGG + chr5.3:5574377-5574396 None:intergenic 60.0%
AGTTATCGGCGCAGGCAACG+TGG - chr5.3:5574230-5574249 MS.gene025803:CDS 60.0%
CAAGCTACGAGGCGAGATGC+TGG - chr5.3:5574326-5574345 MS.gene025803:CDS 60.0%
CCTCCTCTACCAAGCTGTGC+AGG + chr5.3:5575291-5575310 None:intergenic 60.0%
CCTCTGCATCGTCACTGCTG+GGG - chr5.3:5574428-5574447 MS.gene025803:CDS 60.0%
CCTGCACAGCTTGGTAGAGG+AGG - chr5.3:5575288-5575307 MS.gene025803:CDS 60.0%
CTGAATAGTCCGTGGAGGCC+TGG + chr5.3:5574392-5574411 None:intergenic 60.0%
GACCTCTGCATCGTCACTGC+TGG - chr5.3:5574426-5574445 MS.gene025803:CDS 60.0%
GAGGCCTGGATCTTAGTGCG+GGG + chr5.3:5574378-5574397 None:intergenic 60.0%
GATCTTAGTGCGGGGGAGGA+AGG + chr5.3:5574370-5574389 None:intergenic 60.0%
GCTGGGGCTCGCCAGATTAA+TGG - chr5.3:5574444-5574463 MS.gene025803:CDS 60.0%
GGAGGCCTGGATCTTAGTGC+GGG + chr5.3:5574379-5574398 None:intergenic 60.0%
GTTATCGGCGCAGGCAACGT+GGG - chr5.3:5574231-5574250 MS.gene025803:CDS 60.0%
TTCCCCTCCAACCGCGTCAT+TGG - chr5.3:5574615-5574634 MS.gene025803:intron 60.0%
CCCCAGCAGTGACGATGCAG+AGG + chr5.3:5574431-5574450 None:intergenic 65.0%
CCTCCCCCGCACTAAGATCC+AGG - chr5.3:5574371-5574390 MS.gene025803:CDS 65.0%
CCTGGATCTTAGTGCGGGGG+AGG + chr5.3:5574374-5574393 None:intergenic 65.0%
CGGCGCAGGCAACGTGGGAA+TGG - chr5.3:5574236-5574255 MS.gene025803:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 5574108 5575413 5574108 ID=MS.gene025803
chr5.3 mRNA 5574108 5575413 5574108 ID=MS.gene025803.t1;Parent=MS.gene025803
chr5.3 exon 5574805 5575413 5574805 ID=MS.gene025803.t1.exon1;Parent=MS.gene025803.t1
chr5.3 CDS 5574805 5575413 5574805 ID=cds.MS.gene025803.t1;Parent=MS.gene025803.t1
chr5.3 exon 5574108 5574551 5574108 ID=MS.gene025803.t1.exon2;Parent=MS.gene025803.t1
chr5.3 CDS 5574108 5574551 5574108 ID=cds.MS.gene025803.t1;Parent=MS.gene025803.t1
Gene Sequence

>MS.gene025803

ATGCACAAAAGCATATCAGGTTCATCATTGGGACCAGATGGTTTGGACTTAACTCAGTCCTTCTTCAAGTCCATCCAACATGCCTCACCTCCTTCTCCAACGAAACGCCACAACAAGATCTCAGTTATCGGCGCAGGCAACGTGGGAATGGCTATAGCTCAAACCATCTTAACACAAGACCTAGTCGACGAGCTTGTTCTTGTCGACACCATTCCTGACAAGCTACGAGGCGAGATGCTGGATCTACAGCACGCGGCAGCCTTCCTCCCCCGCACTAAGATCCAGGCCTCCACGGACTATTCAGTGACGATGGGGTCTGACCTCTGCATCGTCACTGCTGGGGCTCGCCAGATTAATGGCGAGTCCAGATTGAATTTGCTACAAAGGAATGTTGCTTTGTTTAAAAAAATTATACCACCTTTGGTTCGTTACTCTCCTCATTGTGTTCTTCTAATTGTTTCCAATCCTGTTGATGTGCTCACTTACATTGCTTGGAAACTCTCTGGTTTCCCCTCCAACCGCGTCATTGGTTCAGGAACTAATTTGGACTCCTCTCGCTTCCGTTTCCTTATTGCTGATCATCTTGATGTTAATGCTCAAGATGTGCAGGTACAACTTTTTTCCTTATTGCTTAAAAAATATATATCGTCGATCATTCCCCATTGCACTTGCGCGCATGTTTGGTAATTGGTATCACAGTGAATAGTTTTTCCAAAATCGCAGCCGTAATTTTAGCAAACTCACTATAATACCAAACATGCAATGTAATAGCAATTGCAAAACGTAATTTAGAACTAACACTGATCACGAACGAACACACATAGTAAACTCAATATTTTATCAGTTTAATTTCATATTGTAGGCATACATAGTGGGGGAACATGGTGACAGTTCAGTGGCACTATGGTCAAGCATTAGTGTTGGAGGTGTTCCAGTGCTAAGCTTTTTGGAGAAACAACAAATAGCTTACGAGAAAGAAATGCTAGAGAACATACACAAAGAGGTTATAAATGGCGCATATGAAGTGATCAGTTTAAAAGGCTACACTTCTTGGGCCATAGGGTACTCCGTCGCAAATTTGGCTCGAACAATTATGAGGAATCAAAGGAAGATTCACCCTGTTTCAGTACTTGCAAAGGGATTTTATGGCATTGATGGTGGAGAAGTGTTTATTAGTTTGCCTGCACAGCTTGGTAGAGGAGGTGTATTGGGTGTGACTAATATTCATATGACTGAAGAGGAAATGAAGAGGCTTAGGGACTCTGCTATCACTATTCTTGAGTTGCAGAATCAGTTGGGTATTTGA
Protein sequence

>MS.gene025803.t1

MHKSISGSSLGPDGLDLTQSFFKSIQHASPPSPTKRHNKISVIGAGNVGMAIAQTILTQDLVDELVLVDTIPDKLRGEMLDLQHAAAFLPRTKIQASTDYSVTMGSDLCIVTAGARQINGESRLNLLQRNVALFKKIIPPLVRYSPHCVLLIVSNPVDVLTYIAWKLSGFPSNRVIGSGTNLDSSRFRFLIADHLDVNAQDVQAYIVGEHGDSSVALWSSISVGGVPVLSFLEKQQIAYEKEMLENIHKEVINGAYEVISLKGYTSWAIGYSVANLARTIMRNQRKIHPVSVLAKGFYGIDGGEVFISLPAQLGRGGVLGVTNIHMTEEEMKRLRDSAITILELQNQLGI