AlfalfaGEDB | MS.gene030942

M. sativa cultivar XinJiangDaYe / MS.gene030942

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030942.t1	XP_003629958.1	97.3	148	4	0	1	148	1	148	2.70E-75	291.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030942.t1	Q43517	67.1	143	47	0	6	148	2	144	3.6e-52	205.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030942.t1	G7L8U4	97.3	148	4	0	1	148	1	148	2.0e-75	291.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene21845	MS.gene030942	PPI
MS.gene034453	MS.gene030942	PPI
MS.gene067740	MS.gene030942	PPI
MS.gene030942	MS.gene000152	PPI
MS.gene84322	MS.gene030942	PPI
MS.gene030942	MS.gene88235	PPI
MS.gene74977	MS.gene030942	PPI
MS.gene030942	MS.gene35417	PPI
MS.gene030942	MS.gene66754	PPI
MS.gene030942	MS.gene03847	PPI
MS.gene030942	MS.gene029328	PPI
MS.gene030942	MS.gene43037	PPI
MS.gene92294	MS.gene030942	PPI
MS.gene030942	MS.gene024872	PPI
MS.gene065061	MS.gene030942	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030942.t1	MTR_8g088720	97.297	148	4	0	1	148	1	148	7.18e-107	301
MS.gene030942.t1	MTR_2g006290	63.816	152	44	3	4	148	3	150	1.87e-63	191
MS.gene030942.t1	MTR_1g112030	56.164	146	57	5	4	146	18	159	1.57e-50	159
MS.gene030942.t1	MTR_1g112030	56.164	146	57	5	4	146	14	155	1.65e-50	159
MS.gene030942.t1	MTR_1g112050	55.782	147	62	3	1	146	9	153	6.20e-50	157
MS.gene030942.t1	MTR_2g086510	61.789	123	45	2	25	146	33	154	1.05e-49	156
MS.gene030942.t1	MTR_4g053120	57.037	135	51	3	19	146	20	154	5.77e-48	152
MS.gene030942.t1	MTR_5g006320	36.634	101	63	1	48	148	46	145	1.67e-17	74.7
MS.gene030942.t1	MTR_4g098490	36.585	82	52	0	65	146	77	158	3.29e-16	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030942.t1	AT1G60950	64.000	150	49	3	1	147	1	148	1.03e-65	197
MS.gene030942.t1	AT1G10960	61.333	150	53	3	1	147	1	148	5.54e-59	180
MS.gene030942.t1	AT2G27510	50.658	152	65	4	5	146	3	154	1.99e-44	143
MS.gene030942.t1	AT5G10000	43.182	132	71	3	19	147	18	148	1.23e-32	113
MS.gene030942.t1	AT4G14890	35.333	150	80	4	3	148	10	146	7.54e-17	72.8
MS.gene030942.t1	AT1G32550	40.244	82	49	0	65	146	72	153	1.26e-16	73.2
MS.gene030942.t1	AT1G32550	34.783	92	44	2	71	146	75	166	5.22e-12	60.8

Find 56 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGTGGGTCAGACTTTATT+TGG	0.300570	4.3:+11480147	MS.gene030942:CDS
CCACGTTAGGGAATGCTGTT+AGG	0.301806	4.3:-11480131	None:intergenic
GTAAGTTCTTTAGTTCCATC+TGG	0.311839	4.3:-11480234	None:intergenic
GCTGTTAGGGTTGTGTTCAT+TGG	0.358114	4.3:-11480117	None:intergenic
ACCACCACAGCAGCCCTATC+AGG	0.384793	4.3:+11480060	MS.gene030942:CDS
TGCTCACCATGGTTCCTGAT+AGG	0.397541	4.3:-11480074	None:intergenic
TGTGCTGGAAAAGTTGTAGA+AGG	0.411785	4.3:+11480345	MS.gene030942:CDS
AATAAAGTCTGACCCACGTT+AGG	0.420347	4.3:-11480144	None:intergenic
TCGAGAATGTACTCATTATC+CGG	0.426007	4.3:-11480261	None:intergenic
GATGAGCTTGATCTTGTAAC+TGG	0.426566	4.3:-11480208	None:intergenic
CTTCCTTACTCATGCAGGGC+TGG	0.437541	4.3:+11480309	MS.gene030942:CDS
GGTGCTTGCTCTTCATGTGC+TGG	0.439901	4.3:+11480330	MS.gene030942:CDS
TCGATCTTCCTTACTCATGC+AGG	0.452165	4.3:+11480304	MS.gene030942:CDS
TAAGTTCTTTAGTTCCATCT+GGG	0.455484	4.3:-11480233	None:intergenic
GCTCACCATGGTTCCTGATA+GGG	0.466904	4.3:-11480073	None:intergenic
TCCTGATAGGGCTGCTGTGG+TGG	0.470793	4.3:-11480061	None:intergenic
CATTGGCTGCCTCCTGATGA+AGG	0.472321	4.3:-11480100	None:intergenic
ACTTTATTTGGTGTGAAGGC+CGG	0.475879	4.3:+11480159	MS.gene030942:CDS
TGATAGGGCTGCTGTGGTGG+TGG	0.476979	4.3:-11480058	None:intergenic
TGGTGAGCACCTCCTTCATC+AGG	0.483290	4.3:+11480088	MS.gene030942:CDS
CACGTTAGGGAATGCTGTTA+GGG	0.501804	4.3:-11480130	None:intergenic
GCACCAGCCCTGCATGAGTA+AGG	0.502602	4.3:-11480312	None:intergenic
GATGATGATCAATTAAGTGC+TGG	0.507761	4.3:+11480399	MS.gene030942:CDS
TCAGACTTTATTTGGTGTGA+AGG	0.514081	4.3:+11480155	MS.gene030942:CDS
ATAAAGTCTGACCCACGTTA+GGG	0.523812	4.3:-11480143	None:intergenic
AAAAGTTGTAGAAGGGGAAG+TGG	0.534366	4.3:+11480353	MS.gene030942:CDS
ATCAAGCTCATCACCCCAGA+TGG	0.540257	4.3:+11480219	MS.gene030942:CDS
CTTGATCTTGTAACTGGCCA+TGG	0.544201	4.3:-11480202	None:intergenic
ATGATGATCAATTAAGTGCT+GGG	0.551795	4.3:+11480400	MS.gene030942:CDS
AAGATCGATGCCTTGTTCCT+CGG	0.553403	4.3:-11480289	None:intergenic
CTCGACGTAGCCGAGGAACA+AGG	0.555180	4.3:+11480279	MS.gene030942:CDS
CACTTAATTGATCATCATCA+AGG	0.556300	4.3:-11480395	None:intergenic
AACTAAAGAACTTACCTGCC+CGG	0.559896	4.3:+11480242	MS.gene030942:CDS
TTTGGTGTGAAGGCCGGACG+TGG	0.560810	4.3:+11480165	MS.gene030942:CDS
AATGTACTCATTATCCGGGC+AGG	0.566913	4.3:-11480256	None:intergenic
CGATCTTCCTTACTCATGCA+GGG	0.578511	4.3:+11480305	MS.gene030942:CDS
GTGCTGGAAAAGTTGTAGAA+GGG	0.579438	4.3:+11480346	MS.gene030942:CDS
GGTTCCTGATAGGGCTGCTG+TGG	0.579627	4.3:-11480064	None:intergenic
AGTGATACGACTTCCACGTC+CGG	0.584494	4.3:-11480178	None:intergenic
CGAGAATGTACTCATTATCC+GGG	0.584894	4.3:-11480260	None:intergenic
CCTTGTGTGTCTCAATAACA+AGG	0.591064	4.3:-11480458	None:intergenic
CCTAACAGCATTCCCTAACG+TGG	0.597665	4.3:+11480131	MS.gene030942:CDS
TGCTGGAAAAGTTGTAGAAG+GGG	0.598351	4.3:+11480347	MS.gene030942:CDS
TGGAAGTCGTATCACTGCCA+TGG	0.614707	4.3:+11480185	MS.gene030942:CDS
GTACATTCTCGACGTAGCCG+AGG	0.630338	4.3:+11480272	MS.gene030942:CDS
AAGTTCTTTAGTTCCATCTG+GGG	0.632400	4.3:-11480232	None:intergenic
AGCAGCCCTATCAGGAACCA+TGG	0.637647	4.3:+11480068	MS.gene030942:CDS
TGGCTGCCTCCTGATGAAGG+AGG	0.644050	4.3:-11480097	None:intergenic
GTGTGTCTCAATAACAAGGT+CGG	0.646026	4.3:-11480454	None:intergenic
CCTTGTTATTGAGACACACA+AGG	0.649100	4.3:+11480458	MS.gene030942:CDS
GATGAAGGAGGTGCTCACCA+TGG	0.650251	4.3:-11480085	None:intergenic
TCAATAACAAGGTCGGAACG+AGG	0.657316	4.3:-11480447	None:intergenic
CAATAACAAGGTCGGAACGA+GGG	0.670427	4.3:-11480446	None:intergenic
TGAGCACCTCCTTCATCAGG+AGG	0.676610	4.3:+11480091	MS.gene030942:CDS
CTAACAGCATTCCCTAACGT+GGG	0.718396	4.3:+11480132	MS.gene030942:CDS
TGTTATTGAGACACACAAGG+AGG	0.740119	4.3:+11480461	MS.gene030942:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGATGATCAATTAAGTGCT+GGG	+	chr4.3:11480400-11480419	MS.gene030942:CDS	30.0%
	CACTTAATTGATCATCATCA+AGG	-	chr4.3:11480398-11480417	None:intergenic	30.0%
!	TAAGTTCTTTAGTTCCATCT+GGG	-	chr4.3:11480236-11480255	None:intergenic	30.0%
	GATGATGATCAATTAAGTGC+TGG	+	chr4.3:11480399-11480418	MS.gene030942:CDS	35.0%
	TCGAGAATGTACTCATTATC+CGG	-	chr4.3:11480264-11480283	None:intergenic	35.0%
!	AAGTTCTTTAGTTCCATCTG+GGG	-	chr4.3:11480235-11480254	None:intergenic	35.0%
!	GTAAGTTCTTTAGTTCCATC+TGG	-	chr4.3:11480237-11480256	None:intergenic	35.0%
!!	TCAGACTTTATTTGGTGTGA+AGG	+	chr4.3:11480155-11480174	MS.gene030942:CDS	35.0%
	AAAAGTTGTAGAAGGGGAAG+TGG	+	chr4.3:11480353-11480372	MS.gene030942:CDS	40.0%
	AACGTGGGTCAGACTTTATT+TGG	+	chr4.3:11480147-11480166	MS.gene030942:CDS	40.0%
	AACTAAAGAACTTACCTGCC+CGG	+	chr4.3:11480242-11480261	MS.gene030942:CDS	40.0%
	AATAAAGTCTGACCCACGTT+AGG	-	chr4.3:11480147-11480166	None:intergenic	40.0%
	ATAAAGTCTGACCCACGTTA+GGG	-	chr4.3:11480146-11480165	None:intergenic	40.0%
	CCTTGTGTGTCTCAATAACA+AGG	-	chr4.3:11480461-11480480	None:intergenic	40.0%
	CGAGAATGTACTCATTATCC+GGG	-	chr4.3:11480263-11480282	None:intergenic	40.0%
	GATGAGCTTGATCTTGTAAC+TGG	-	chr4.3:11480211-11480230	None:intergenic	40.0%
	GTGCTGGAAAAGTTGTAGAA+GGG	+	chr4.3:11480346-11480365	MS.gene030942:CDS	40.0%
	GTGTGTCTCAATAACAAGGT+CGG	-	chr4.3:11480457-11480476	None:intergenic	40.0%
	TGCTGGAAAAGTTGTAGAAG+GGG	+	chr4.3:11480347-11480366	MS.gene030942:CDS	40.0%
	TGTGCTGGAAAAGTTGTAGA+AGG	+	chr4.3:11480345-11480364	MS.gene030942:CDS	40.0%
	TGTTATTGAGACACACAAGG+AGG	+	chr4.3:11480461-11480480	MS.gene030942:CDS	40.0%
!!	ACTTTATTTGGTGTGAAGGC+CGG	+	chr4.3:11480159-11480178	MS.gene030942:CDS	40.0%
!!	CCTTGTTATTGAGACACACA+AGG	+	chr4.3:11480458-11480477	MS.gene030942:CDS	40.0%
	AATGTACTCATTATCCGGGC+AGG	-	chr4.3:11480259-11480278	None:intergenic	45.0%
	CAATAACAAGGTCGGAACGA+GGG	-	chr4.3:11480449-11480468	None:intergenic	45.0%
	CACGTTAGGGAATGCTGTTA+GGG	-	chr4.3:11480133-11480152	None:intergenic	45.0%
	CGATCTTCCTTACTCATGCA+GGG	+	chr4.3:11480305-11480324	MS.gene030942:CDS	45.0%
	CTAACAGCATTCCCTAACGT+GGG	+	chr4.3:11480132-11480151	MS.gene030942:CDS	45.0%
	CTTGATCTTGTAACTGGCCA+TGG	-	chr4.3:11480205-11480224	None:intergenic	45.0%
	GCTGTTAGGGTTGTGTTCAT+TGG	-	chr4.3:11480120-11480139	None:intergenic	45.0%
	TCAATAACAAGGTCGGAACG+AGG	-	chr4.3:11480450-11480469	None:intergenic	45.0%
	TCGATCTTCCTTACTCATGC+AGG	+	chr4.3:11480304-11480323	MS.gene030942:CDS	45.0%
!	AAGATCGATGCCTTGTTCCT+CGG	-	chr4.3:11480292-11480311	None:intergenic	45.0%
	AGTGATACGACTTCCACGTC+CGG	-	chr4.3:11480181-11480200	None:intergenic	50.0%
	ATCAAGCTCATCACCCCAGA+TGG	+	chr4.3:11480219-11480238	MS.gene030942:CDS	50.0%
	CCACGTTAGGGAATGCTGTT+AGG	-	chr4.3:11480134-11480153	None:intergenic	50.0%
	CCTAACAGCATTCCCTAACG+TGG	+	chr4.3:11480131-11480150	MS.gene030942:CDS	50.0%
	GCTCACCATGGTTCCTGATA+GGG	-	chr4.3:11480076-11480095	None:intergenic	50.0%
	TGCTCACCATGGTTCCTGAT+AGG	-	chr4.3:11480077-11480096	None:intergenic	50.0%
	TGGAAGTCGTATCACTGCCA+TGG	+	chr4.3:11480185-11480204	MS.gene030942:CDS	50.0%
	AGCAGCCCTATCAGGAACCA+TGG	+	chr4.3:11480068-11480087	MS.gene030942:CDS	55.0%
	CTTCCTTACTCATGCAGGGC+TGG	+	chr4.3:11480309-11480328	MS.gene030942:CDS	55.0%
	GGTGCTTGCTCTTCATGTGC+TGG	+	chr4.3:11480330-11480349	MS.gene030942:CDS	55.0%
	GTACATTCTCGACGTAGCCG+AGG	+	chr4.3:11480272-11480291	MS.gene030942:CDS	55.0%
	TGAGCACCTCCTTCATCAGG+AGG	+	chr4.3:11480091-11480110	MS.gene030942:CDS	55.0%
	TGGTGAGCACCTCCTTCATC+AGG	+	chr4.3:11480088-11480107	MS.gene030942:CDS	55.0%
!	CATTGGCTGCCTCCTGATGA+AGG	-	chr4.3:11480103-11480122	None:intergenic	55.0%
!!	GATGAAGGAGGTGCTCACCA+TGG	-	chr4.3:11480088-11480107	None:intergenic	55.0%
	ACCACCACAGCAGCCCTATC+AGG	+	chr4.3:11480060-11480079	MS.gene030942:CDS	60.0%
	CTCGACGTAGCCGAGGAACA+AGG	+	chr4.3:11480279-11480298	MS.gene030942:CDS	60.0%
	GCACCAGCCCTGCATGAGTA+AGG	-	chr4.3:11480315-11480334	None:intergenic	60.0%
	GGTTCCTGATAGGGCTGCTG+TGG	-	chr4.3:11480067-11480086	None:intergenic	60.0%
	TCCTGATAGGGCTGCTGTGG+TGG	-	chr4.3:11480064-11480083	None:intergenic	60.0%
	TGATAGGGCTGCTGTGGTGG+TGG	-	chr4.3:11480061-11480080	None:intergenic	60.0%
	TGGCTGCCTCCTGATGAAGG+AGG	-	chr4.3:11480100-11480119	None:intergenic	60.0%
!	TTTGGTGTGAAGGCCGGACG+TGG	+	chr4.3:11480165-11480184	MS.gene030942:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	11480054	11480500	11480054	ID=MS.gene030942
chr4.3	mRNA	11480054	11480500	11480054	ID=MS.gene030942.t1;Parent=MS.gene030942
chr4.3	exon	11480054	11480500	11480054	ID=MS.gene030942.t1.exon1;Parent=MS.gene030942.t1
chr4.3	CDS	11480054	11480500	11480054	ID=cds.MS.gene030942.t1;Parent=MS.gene030942.t1

Gene Sequence

>MS.gene030942

ATGGCCACCACCACAGCAGCCCTATCAGGAACCATGGTGAGCACCTCCTTCATCAGGAGGCAGCCAATGAACACAACCCTAACAGCATTCCCTAACGTGGGTCAGACTTTATTTGGTGTGAAGGCCGGACGTGGAAGTCGTATCACTGCCATGGCCAGTTACAAGATCAAGCTCATCACCCCAGATGGAACTAAAGAACTTACCTGCCCGGATAATGAGTACATTCTCGACGTAGCCGAGGAACAAGGCATCGATCTTCCTTACTCATGCAGGGCTGGTGCTTGCTCTTCATGTGCTGGAAAAGTTGTAGAAGGGGAAGTGGATAATTCAGATAATAGCTTCCTTGATGATGATCAATTAAGTGCTGGGTTTGTTCTCACATGTGTTGCTTACCCTCGTTCCGACCTTGTTATTGAGACACACAAGGAGGAAGATCTTGTTTCTTAA

Protein sequence

>MS.gene030942.t1

MATTTAALSGTMVSTSFIRRQPMNTTLTAFPNVGQTLFGVKAGRGSRITAMASYKIKLITPDGTKELTCPDNEYILDVAEEQGIDLPYSCRAGACSSCAGKVVEGEVDNSDNSFLDDDQLSAGFVLTCVAYPRSDLVIETHKEEDLVS