AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92294


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92294.t1 MTR_5g006320 94.805 154 4 2 1 151 1 153 2.58e-103 292
MS.gene92294.t1 MTR_2g086510 34.416 154 83 4 7 142 2 155 6.62e-23 88.6
MS.gene92294.t1 MTR_4g053120 33.113 151 91 4 1 141 4 154 2.57e-22 87.0
MS.gene92294.t1 MTR_1g112050 37.302 126 72 4 22 141 29 153 3.72e-19 79.0
MS.gene92294.t1 MTR_1g112030 38.655 119 62 3 34 141 37 155 4.15e-19 79.0
MS.gene92294.t1 MTR_1g112030 38.655 119 62 3 34 141 41 159 4.86e-19 79.0
MS.gene92294.t1 MTR_2g006290 31.387 137 86 3 10 141 15 148 2.59e-18 76.6
MS.gene92294.t1 MTR_8g088720 34.400 125 77 2 20 143 28 148 7.77e-18 75.5
MS.gene92294.t1 MTR_4g098490 32.759 116 71 3 33 147 55 164 2.88e-14 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92294.t1 AT4G14890 81.132 106 20 0 46 151 49 154 5.44e-62 187
MS.gene92294.t1 AT2G27510 34.395 157 86 5 1 142 1 155 4.27e-23 89.4
MS.gene92294.t1 AT1G60950 34.532 139 81 6 10 142 14 148 2.73e-18 76.6
MS.gene92294.t1 AT5G10000 32.847 137 82 3 16 142 12 148 1.65e-16 72.0
MS.gene92294.t1 AT1G10960 30.435 138 88 4 10 142 14 148 4.84e-15 68.2
MS.gene92294.t1 AT1G32550 31.915 141 88 4 9 147 25 159 4.67e-14 66.2

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCAATGCCTTTGATAATA+TGG 0.193692 5.1:+1140540 None:intergenic
TCGTTCTGATTGTCATATTA+AGG 0.194811 5.1:-1140368 MS.gene92294:CDS
TTCTGTCTGAAGAGAGTTAA+GGG 0.376635 5.1:+1140727 None:intergenic
TTTGATAATATGGTTTCATC+AGG 0.398760 5.1:+1140550 None:intergenic
CTGAAAGCTAAATTAGTCAT+TGG 0.415804 5.1:+1140303 None:intergenic
CTTCTGTCTGAAGAGAGTTA+AGG 0.435022 5.1:+1140726 None:intergenic
AGGATAGGGGAGCTTAGTTG+TGG 0.462415 5.1:+1140696 None:intergenic
GAGTTAAGGGTGTAAATTGA+AGG 0.462964 5.1:+1140740 None:intergenic
TTATCAAAGGCATTGGACTC+TGG 0.464183 5.1:-1140535 MS.gene92294:CDS
GGATAGGGGAGCTTAGTTGT+GGG 0.466042 5.1:+1140697 None:intergenic
AACCATATTATCAAAGGCAT+TGG 0.476989 5.1:-1140542 MS.gene92294:CDS
TGTGTTGAGCTGAGAAGGAT+AGG 0.480320 5.1:+1140681 None:intergenic
ACAAGACCACGCCTTGGTTC+AGG 0.487317 5.1:-1140661 MS.gene92294:CDS
TGTGAAGTCTTCTTATAAAG+TGG 0.499881 5.1:-1140614 MS.gene92294:CDS
ACAATCAGAACGAGGGTAAG+AGG 0.509900 5.1:+1140378 None:intergenic
GTGCCTCATGACTGTAAGCT+TGG 0.510320 5.1:-1140505 MS.gene92294:CDS
AGTTAAGGGTGTAAATTGAA+GGG 0.533055 5.1:+1140741 None:intergenic
GTGTTGAGCTGAGAAGGATA+GGG 0.540357 5.1:+1140682 None:intergenic
TAAAGTGGTGATTGAGCATG+AGG 0.567310 5.1:-1140599 MS.gene92294:CDS
TGATGAAACCATATTATCAA+AGG 0.569530 5.1:-1140548 MS.gene92294:CDS
GGTCTTGTGTTGAGCTGAGA+AGG 0.574174 5.1:+1140676 None:intergenic
CTCAACACAAGACCACGCCT+TGG 0.584774 5.1:-1140667 MS.gene92294:CDS
GTGCCACTGATAAGACGAGC+AGG 0.599960 5.1:+1140466 None:intergenic
TCATATTAAGGTTATAGCAG+AGG 0.608535 5.1:-1140356 MS.gene92294:CDS
TCACAGTGAAAGTGAGTGAT+GGG 0.611048 5.1:+1140632 None:intergenic
GGCACAGTTGATCAGAGTGA+TGG 0.619808 5.1:-1140448 MS.gene92294:CDS
TTCACAGTGAAAGTGAGTGA+TGG 0.627712 5.1:+1140631 None:intergenic
TGTTGAGCTGAGAAGGATAG+GGG 0.635174 5.1:+1140683 None:intergenic
TGTCCTGCTCGTCTTATCAG+TGG 0.649957 5.1:-1140469 MS.gene92294:CDS
AAGCTTACAGTCATGAGGCA+CGG 0.652590 5.1:+1140507 None:intergenic
GATAGGGGAGCTTAGTTGTG+GGG 0.665612 5.1:+1140698 None:intergenic
AGTGATGATGTTGTAGAGCG+TGG 0.677618 5.1:-1140418 MS.gene92294:CDS
ACTCCAAGCTTACAGTCATG+AGG 0.678735 5.1:+1140502 None:intergenic
TAATATGACAATCAGAACGA+GGG 0.699912 5.1:+1140371 None:intergenic
AAAGTGGTGATTGAGCATGA+GGG 0.724790 5.1:-1140598 MS.gene92294:CDS
TGTGAACCTGAACCAAGGCG+TGG 0.725548 5.1:+1140655 None:intergenic
TTAATATGACAATCAGAACG+AGG 0.733351 5.1:+1140370 None:intergenic
ATGGGTGTGAACCTGAACCA+AGG 0.761073 5.1:+1140650 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATGAAACCATATTATCAA+AGG - chr5.1:1140515-1140534 MS.gene92294:CDS 25.0%
!! TTTGATAATATGGTTTCATC+AGG + chr5.1:1140516-1140535 None:intergenic 25.0%
TAATATGACAATCAGAACGA+GGG + chr5.1:1140695-1140714 None:intergenic 30.0%
TCATATTAAGGTTATAGCAG+AGG - chr5.1:1140707-1140726 MS.gene92294:CDS 30.0%
TCGTTCTGATTGTCATATTA+AGG - chr5.1:1140695-1140714 MS.gene92294:CDS 30.0%
TGTGAAGTCTTCTTATAAAG+TGG - chr5.1:1140449-1140468 MS.gene92294:CDS 30.0%
TTAATATGACAATCAGAACG+AGG + chr5.1:1140696-1140715 None:intergenic 30.0%
! AACCATATTATCAAAGGCAT+TGG - chr5.1:1140521-1140540 MS.gene92294:CDS 30.0%
! AGTTAAGGGTGTAAATTGAA+GGG + chr5.1:1140325-1140344 None:intergenic 30.0%
TTCTGTCTGAAGAGAGTTAA+GGG + chr5.1:1140339-1140358 None:intergenic 35.0%
! GAGTTAAGGGTGTAAATTGA+AGG + chr5.1:1140326-1140345 None:intergenic 35.0%
! GTCCAATGCCTTTGATAATA+TGG + chr5.1:1140526-1140545 None:intergenic 35.0%
AAAGTGGTGATTGAGCATGA+GGG - chr5.1:1140465-1140484 MS.gene92294:CDS 40.0%
CTTCTGTCTGAAGAGAGTTA+AGG + chr5.1:1140340-1140359 None:intergenic 40.0%
TAAAGTGGTGATTGAGCATG+AGG - chr5.1:1140464-1140483 MS.gene92294:CDS 40.0%
TCACAGTGAAAGTGAGTGAT+GGG + chr5.1:1140434-1140453 None:intergenic 40.0%
TTCACAGTGAAAGTGAGTGA+TGG + chr5.1:1140435-1140454 None:intergenic 40.0%
!! TTATCAAAGGCATTGGACTC+TGG - chr5.1:1140528-1140547 MS.gene92294:CDS 40.0%
AAGCTTACAGTCATGAGGCA+CGG + chr5.1:1140559-1140578 None:intergenic 45.0%
ACAATCAGAACGAGGGTAAG+AGG + chr5.1:1140688-1140707 None:intergenic 45.0%
ACTCCAAGCTTACAGTCATG+AGG + chr5.1:1140564-1140583 None:intergenic 45.0%
GTGTTGAGCTGAGAAGGATA+GGG + chr5.1:1140384-1140403 None:intergenic 45.0%
TGTGTTGAGCTGAGAAGGAT+AGG + chr5.1:1140385-1140404 None:intergenic 45.0%
TGTTGAGCTGAGAAGGATAG+GGG + chr5.1:1140383-1140402 None:intergenic 45.0%
! AGTGATGATGTTGTAGAGCG+TGG - chr5.1:1140645-1140664 MS.gene92294:CDS 45.0%
AGGATAGGGGAGCTTAGTTG+TGG + chr5.1:1140370-1140389 None:intergenic 50.0%
ATGGGTGTGAACCTGAACCA+AGG + chr5.1:1140416-1140435 None:intergenic 50.0%
GATAGGGGAGCTTAGTTGTG+GGG + chr5.1:1140368-1140387 None:intergenic 50.0%
GGATAGGGGAGCTTAGTTGT+GGG + chr5.1:1140369-1140388 None:intergenic 50.0%
GGCACAGTTGATCAGAGTGA+TGG - chr5.1:1140615-1140634 MS.gene92294:CDS 50.0%
GTGCCTCATGACTGTAAGCT+TGG - chr5.1:1140558-1140577 MS.gene92294:CDS 50.0%
TGTCCTGCTCGTCTTATCAG+TGG - chr5.1:1140594-1140613 MS.gene92294:CDS 50.0%
! GGTCTTGTGTTGAGCTGAGA+AGG + chr5.1:1140390-1140409 None:intergenic 50.0%
ACAAGACCACGCCTTGGTTC+AGG - chr5.1:1140402-1140421 MS.gene92294:CDS 55.0%
CTCAACACAAGACCACGCCT+TGG - chr5.1:1140396-1140415 MS.gene92294:CDS 55.0%
TGTGAACCTGAACCAAGGCG+TGG + chr5.1:1140411-1140430 None:intergenic 55.0%
! GTGCCACTGATAAGACGAGC+AGG + chr5.1:1140600-1140619 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 1140315 1140770 1140315 ID=MS.gene92294
chr5.1 mRNA 1140315 1140770 1140315 ID=MS.gene92294.t1;Parent=MS.gene92294
chr5.1 exon 1140315 1140770 1140315 ID=MS.gene92294.t1.exon1;Parent=MS.gene92294.t1
chr5.1 CDS 1140315 1140770 1140315 ID=cds.MS.gene92294.t1;Parent=MS.gene92294.t1
Gene Sequence

>MS.gene92294

ATGGCAACCCTTCAATTTACACCCTTAACTCTCTTCAGACAGAAGAAGCACCCCACAACTAAGCTCCCCTATCCTTCTCAGCTCAACACAAGACCACGCCTTGGTTCAGGTTCACACCCATCACTCACTTTCACTGTGAAGTCTTCTTATAAAGTGGTGATTGAGCATGAGGGCAAGACTACACAGCTTGAAGTTGAACCTGATGAAACCATATTATCAAAGGCATTGGACTCTGGTTTGTCCGTGCCTCATGACTGTAAGCTTGGAGTTTGCATGACTTGTCCTGCTCGTCTTATCAGTGGCACAGTTGATCAGAGTGATGGTATGCTCAGTGATGATGTTGTAGAGCGTGGCTATGCACTCTTGTGTGCCTCTTACCCTCGTTCTGATTGTCATATTAAGGTTATAGCAGAGGATGAGCTACTCTCAATGCAGTTAGCTACAGCCAATGACTAA

Protein sequence

>MS.gene92294.t1

MATLQFTPLTLFRQKKHPTTKLPYPSQLNTRPRLGSGSHPSLTFTVKSSYKVVIEHEGKTTQLEVEPDETILSKALDSGLSVPHDCKLGVCMTCPARLISGTVDQSDGMLSDDVVERGYALLCASYPRSDCHIKVIAEDELLSMQLATAND