AlfalfaGEDB | MS.gene88235

M. sativa cultivar XinJiangDaYe / MS.gene88235

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88235.t1	XP_003606105.2	97.4	155	4	0	1	155	1	155	2.70E-78	301.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88235.t1	P27788	62.5	152	57	0	4	155	1	152	4.8e-47	188.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88235.t1	G7JHA6	97.4	155	4	0	1	155	1	155	2.0e-78	301.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene060802	MS.gene88235	-0.809816	1.57E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene88235	MS.gene000152	PPI
MS.gene030942	MS.gene88235	PPI
MS.gene024872	MS.gene88235	PPI
MS.gene47889	MS.gene88235	PPI
MS.gene67232	MS.gene88235	PPI
MS.gene27932	MS.gene88235	PPI
MS.gene92294	MS.gene88235	PPI
MS.gene21845	MS.gene88235	PPI
MS.gene030944	MS.gene88235	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88235.t1	MTR_4g053120	97.419	155	4	0	1	155	1	155	1.30e-110	311
MS.gene88235.t1	MTR_2g086510	74.194	155	40	0	1	155	1	155	3.38e-83	241
MS.gene88235.t1	MTR_1g112030	64.744	156	49	3	4	155	3	156	1.63e-65	197
MS.gene88235.t1	MTR_1g112030	64.744	156	49	3	4	155	7	160	1.74e-65	197
MS.gene88235.t1	MTR_1g112050	63.226	155	51	3	4	154	1	153	1.23e-62	189
MS.gene88235.t1	MTR_2g006290	59.259	135	40	4	24	154	25	148	1.70e-50	159
MS.gene88235.t1	MTR_8g088720	65.138	109	37	1	46	154	39	146	1.51e-47	151
MS.gene88235.t1	MTR_5g006320	33.775	151	92	4	4	154	1	143	9.51e-21	83.2
MS.gene88235.t1	MTR_4g098490	28.767	146	101	1	13	155	14	159	2.16e-17	75.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene88235.t1	AT2G27510	59.355	155	60	2	4	155	1	155	1.28e-61	187
MS.gene88235.t1	AT1G60950	67.568	111	33	2	46	154	38	147	6.59e-49	154
MS.gene88235.t1	AT1G10960	58.209	134	48	4	20	151	17	144	8.23e-47	149
MS.gene88235.t1	AT5G10000	53.465	101	47	0	54	154	47	147	1.18e-38	129
MS.gene88235.t1	AT4G14890	45.263	95	50	2	60	154	52	144	8.45e-22	85.9
MS.gene88235.t1	AT1G32550	37.209	86	54	0	70	155	69	154	1.74e-17	75.5
MS.gene88235.t1	AT1G32550	34.314	102	48	3	70	155	69	167	1.19e-11	60.5

Find 42 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGAAGTTCCCTTCATCTTTC+GGG	0.151505	4.4:+49221559	MS.gene88235:CDS
TGTGCTGGAAAGATTGTTTC+TGG	0.226463	4.4:+49221789	MS.gene88235:CDS
GCTCCAGCACGGCATGAATA+AGG	0.234304	4.4:-49221756	None:intergenic
GCTTACAAGGTCAAGCTTAT+TGG	0.250602	4.4:+49221651	MS.gene88235:CDS
GCGAAGTTCCCTTCATCTTT+CGG	0.263582	4.4:+49221558	MS.gene88235:CDS
TGACTTCAAGCCACAAGATT+TGG	0.293119	4.4:-49221598	None:intergenic
CTTGGATGATAATCAACTTA+AGG	0.362806	4.4:+49221839	MS.gene88235:CDS
TCAATCTGATGGCTCATTCT+TGG	0.370368	4.4:+49221821	MS.gene88235:CDS
AACTGCAATGGCTGCTTACA+AGG	0.410013	4.4:+49221638	MS.gene88235:CDS
CTCCCTTATTCATGCCGTGC+TGG	0.418450	4.4:+49221753	MS.gene88235:CDS
TCTAAGATGTAAACATCATC+GGG	0.429231	4.4:-49221705	None:intergenic
CTCCAGCACGGCATGAATAA+GGG	0.444632	4.4:-49221755	None:intergenic
CAGTTTGAATCATGCATAGC+GGG	0.450595	4.4:-49221503	None:intergenic
GGAGCTTGCTCTACTTGTGC+TGG	0.452619	4.4:+49221774	MS.gene88235:CDS
TGGAAAGATTGTTTCTGGCT+CGG	0.490169	4.4:+49221794	MS.gene88235:CDS
TATGCAATCTGCGGTTGGAT+AGG	0.494449	4.4:-49221886	None:intergenic
GTCAAGCTTATTGGACCAGA+TGG	0.514160	4.4:+49221660	MS.gene88235:CDS
TTTGTAGACCCGAAAGATGA+AGG	0.518911	4.4:-49221567	None:intergenic
ATCTAAGATGTAAACATCAT+CGG	0.524266	4.4:-49221706	None:intergenic
GGCTCGGTGGATCAATCTGA+TGG	0.542825	4.4:+49221810	MS.gene88235:CDS
AAGAGTGTTTCCAAATCTTG+TGG	0.543692	4.4:+49221588	MS.gene88235:CDS
GATGATAATCAACTTAAGGA+TGG	0.547500	4.4:+49221843	MS.gene88235:CDS
TCAATTATGCAATCTGCGGT+TGG	0.549164	4.4:-49221891	None:intergenic
TTAGATGCAGCAGAGGATGC+CGG	0.589423	4.4:+49221723	MS.gene88235:CDS
TAGCAATGGTTGATTGATTG+CGG	0.600774	4.4:-49221530	None:intergenic
TTCATATAGAACAACTGCAA+TGG	0.601127	4.4:+49221626	MS.gene88235:CDS
GAACTTCGCTGTGGTAGCAA+TGG	0.602045	4.4:-49221544	None:intergenic
TTGTAGACCCGAAAGATGAA+GGG	0.611828	4.4:-49221566	None:intergenic
ATAATTGAAACTCACAAGGA+AGG	0.616403	4.4:+49221906	MS.gene88235:CDS
AAAGATTGTTTCTGGCTCGG+TGG	0.616952	4.4:+49221797	MS.gene88235:CDS
GGAAACATTCACAGCTGACA+AGG	0.632858	4.4:-49221481	None:intergenic
TTACATCTTAGATGCAGCAG+AGG	0.636425	4.4:+49221716	MS.gene88235:CDS
AAGTAGAGCAAGCTCCAGCA+CGG	0.637088	4.4:-49221767	None:intergenic
TGAATAAGGGAGTTCAACAC+CGG	0.645101	4.4:-49221742	None:intergenic
TGCGGTTGGATAGGAAACAC+AGG	0.661542	4.4:-49221877	None:intergenic
AGTTTCAATTATGCAATCTG+CGG	0.680241	4.4:-49221895	None:intergenic
GCAGTTTGAATCATGCATAG+CGG	0.684816	4.4:-49221504	None:intergenic
TTGCATAATTGAAACTCACA+AGG	0.686967	4.4:+49221902	MS.gene88235:CDS
TAAGATGTAAACATCATCGG+GGG	0.710033	4.4:-49221703	None:intergenic
AGTTTGAATCATGCATAGCG+GGG	0.714486	4.4:-49221502	None:intergenic
AGATGAAGGGAACTTCGCTG+TGG	0.735830	4.4:-49221553	None:intergenic
CTAAGATGTAAACATCATCG+GGG	0.747899	4.4:-49221704	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCTAAGATGTAAACATCAT+CGG	-	chr4.4:49221709-49221728	None:intergenic	25.0%
	AGTTTCAATTATGCAATCTG+CGG	-	chr4.4:49221898-49221917	None:intergenic	30.0%
	ATAATTGAAACTCACAAGGA+AGG	+	chr4.4:49221906-49221925	MS.gene88235:CDS	30.0%
	CTTGGATGATAATCAACTTA+AGG	+	chr4.4:49221839-49221858	MS.gene88235:CDS	30.0%
	GATGATAATCAACTTAAGGA+TGG	+	chr4.4:49221843-49221862	MS.gene88235:CDS	30.0%
	TTCATATAGAACAACTGCAA+TGG	+	chr4.4:49221626-49221645	MS.gene88235:CDS	30.0%
	TTGCATAATTGAAACTCACA+AGG	+	chr4.4:49221902-49221921	MS.gene88235:CDS	30.0%
!	TCTAAGATGTAAACATCATC+GGG	-	chr4.4:49221708-49221727	None:intergenic	30.0%
	CTAAGATGTAAACATCATCG+GGG	-	chr4.4:49221707-49221726	None:intergenic	35.0%
	TAAGATGTAAACATCATCGG+GGG	-	chr4.4:49221706-49221725	None:intergenic	35.0%
	TAGCAATGGTTGATTGATTG+CGG	-	chr4.4:49221533-49221552	None:intergenic	35.0%
!	AACTCATTCTCTTTTCCATC+TGG	-	chr4.4:49221678-49221697	None:intergenic	35.0%
!!	AAGAGTGTTTCCAAATCTTG+TGG	+	chr4.4:49221588-49221607	MS.gene88235:CDS	35.0%
	AGTTTGAATCATGCATAGCG+GGG	-	chr4.4:49221505-49221524	None:intergenic	40.0%
	CAGTTTGAATCATGCATAGC+GGG	-	chr4.4:49221506-49221525	None:intergenic	40.0%
	GCAGTTTGAATCATGCATAG+CGG	-	chr4.4:49221507-49221526	None:intergenic	40.0%
	GCTTACAAGGTCAAGCTTAT+TGG	+	chr4.4:49221651-49221670	MS.gene88235:CDS	40.0%
	TCAATCTGATGGCTCATTCT+TGG	+	chr4.4:49221821-49221840	MS.gene88235:CDS	40.0%
	TCAATTATGCAATCTGCGGT+TGG	-	chr4.4:49221894-49221913	None:intergenic	40.0%
	TGAATAAGGGAGTTCAACAC+CGG	-	chr4.4:49221745-49221764	None:intergenic	40.0%
	TGACTTCAAGCCACAAGATT+TGG	-	chr4.4:49221601-49221620	None:intergenic	40.0%
	TGTGCTGGAAAGATTGTTTC+TGG	+	chr4.4:49221789-49221808	MS.gene88235:CDS	40.0%
	TTACATCTTAGATGCAGCAG+AGG	+	chr4.4:49221716-49221735	MS.gene88235:CDS	40.0%
	TTGTAGACCCGAAAGATGAA+GGG	-	chr4.4:49221569-49221588	None:intergenic	40.0%
	TTTGTAGACCCGAAAGATGA+AGG	-	chr4.4:49221570-49221589	None:intergenic	40.0%
!!	TGGAAAGATTGTTTCTGGCT+CGG	+	chr4.4:49221794-49221813	MS.gene88235:CDS	40.0%
	AACTGCAATGGCTGCTTACA+AGG	+	chr4.4:49221638-49221657	MS.gene88235:CDS	45.0%
	CGAAGTTCCCTTCATCTTTC+GGG	+	chr4.4:49221559-49221578	MS.gene88235:CDS	45.0%
	GCGAAGTTCCCTTCATCTTT+CGG	+	chr4.4:49221558-49221577	MS.gene88235:CDS	45.0%
	GGAAACATTCACAGCTGACA+AGG	-	chr4.4:49221484-49221503	None:intergenic	45.0%
	GTCAAGCTTATTGGACCAGA+TGG	+	chr4.4:49221660-49221679	MS.gene88235:CDS	45.0%
	TATGCAATCTGCGGTTGGAT+AGG	-	chr4.4:49221889-49221908	None:intergenic	45.0%
!!	AAAGATTGTTTCTGGCTCGG+TGG	+	chr4.4:49221797-49221816	MS.gene88235:CDS	45.0%
	AAGTAGAGCAAGCTCCAGCA+CGG	-	chr4.4:49221770-49221789	None:intergenic	50.0%
	AGATGAAGGGAACTTCGCTG+TGG	-	chr4.4:49221556-49221575	None:intergenic	50.0%
	CTCCAGCACGGCATGAATAA+GGG	-	chr4.4:49221758-49221777	None:intergenic	50.0%
	GAACTTCGCTGTGGTAGCAA+TGG	-	chr4.4:49221547-49221566	None:intergenic	50.0%
	TGCGGTTGGATAGGAAACAC+AGG	-	chr4.4:49221880-49221899	None:intergenic	50.0%
	TTAGATGCAGCAGAGGATGC+CGG	+	chr4.4:49221723-49221742	MS.gene88235:CDS	50.0%
	CTCCCTTATTCATGCCGTGC+TGG	+	chr4.4:49221753-49221772	MS.gene88235:CDS	55.0%
	GCTCCAGCACGGCATGAATA+AGG	-	chr4.4:49221759-49221778	None:intergenic	55.0%
!	GGAGCTTGCTCTACTTGTGC+TGG	+	chr4.4:49221774-49221793	MS.gene88235:CDS	55.0%
!!	GGCTCGGTGGATCAATCTGA+TGG	+	chr4.4:49221810-49221829	MS.gene88235:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	49221474	49221941	49221474	ID=MS.gene88235
chr4.4	mRNA	49221474	49221941	49221474	ID=MS.gene88235.t1;Parent=MS.gene88235
chr4.4	exon	49221474	49221941	49221474	ID=MS.gene88235.t1.exon1;Parent=MS.gene88235.t1
chr4.4	CDS	49221474	49221941	49221474	ID=cds.MS.gene88235.t1;Parent=MS.gene88235.t1

Gene Sequence

>MS.gene88235

ATGGCATCCTTGTCAGCTGTGAATGTTTCCCCGCTATGCATGATTCAAACTGCAAACCGCAATCAATCAACCATTGCTACCACAGCGAAGTTCCCTTCATCTTTCGGGTCTACAAAGAGTGTTTCCAAATCTTGTGGCTTGAAGTCATCATCTTCATATAGAACAACTGCAATGGCTGCTTACAAGGTCAAGCTTATTGGACCAGATGGAAAAGAGAATGAGTTCGATGCCCCCGATGATGTTTACATCTTAGATGCAGCAGAGGATGCCGGTGTTGAACTCCCTTATTCATGCCGTGCTGGAGCTTGCTCTACTTGTGCTGGAAAGATTGTTTCTGGCTCGGTGGATCAATCTGATGGCTCATTCTTGGATGATAATCAACTTAAGGATGGCTTTGTGCTTACCTGTGTTTCCTATCCAACCGCAGATTGCATAATTGAAACTCACAAGGAAGGTGATCTCTACTGA

Protein sequence

>MS.gene88235.t1

MASLSAVNVSPLCMIQTANRNQSTIATTAKFPSSFGSTKSVSKSCGLKSSSSYRTTAMAAYKVKLIGPDGKENEFDAPDDVYILDAAEDAGVELPYSCRAGACSTCAGKIVSGSVDQSDGSFLDDNQLKDGFVLTCVSYPTADCIIETHKEGDLY