AlfalfaGEDB | MS.gene065061

M. sativa cultivar XinJiangDaYe / MS.gene065061

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065061.t1	XP_013470266.1	97.4	154	4	0	1	154	7	160	1.70E-75	292

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065061.t1	P27788	65.1	152	50	3	6	154	1	152	1.7e-44	180.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065061.t1	I3SQM0	97.4	154	4	0	1	154	3	156	1.2e-75	292.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050623	MS.gene065061	0.825159	5.46E-54	-1.69E-46
MS.gene050859	MS.gene065061	0.805517	1.28E-49	-1.69E-46
MS.gene052077	MS.gene065061	-0.815804	7.66E-52	-1.69E-46
MS.gene052420	MS.gene065061	-0.831074	2.05E-55	-1.69E-46
MS.gene053836	MS.gene065061	-0.801587	8.39E-49	-1.69E-46
MS.gene054930	MS.gene065061	-0.804602	2.00E-49	-1.69E-46
MS.gene055505	MS.gene065061	-0.818178	2.24E-52	-1.69E-46
MS.gene056979	MS.gene065061	-0.811789	5.87E-51	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene92294	MS.gene065061	PPI
MS.gene065061	MS.gene024872	PPI
MS.gene065061	MS.gene21845	PPI
MS.gene065061	MS.gene000152	PPI
MS.gene67232	MS.gene065061	PPI
MS.gene065061	MS.gene27932	PPI
MS.gene065061	MS.gene030944	PPI
MS.gene47889	MS.gene065061	PPI
MS.gene065061	MS.gene030942	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065061.t1	MTR_1g112030	97.403	154	4	0	1	154	3	156	2.99e-109	307
MS.gene065061.t1	MTR_1g112030	97.403	154	4	0	1	154	7	160	4.28e-109	307
MS.gene065061.t1	MTR_1g112050	90.196	153	15	0	1	153	1	153	1.15e-100	286
MS.gene065061.t1	MTR_2g086510	72.727	132	35	1	24	154	24	155	3.62e-67	201
MS.gene065061.t1	MTR_4g053120	65.385	156	48	3	1	154	4	155	1.22e-65	197
MS.gene065061.t1	MTR_2g006290	55.102	147	60	3	12	153	3	148	7.50e-50	157
MS.gene065061.t1	MTR_8g088720	55.556	144	61	3	12	153	4	146	1.06e-49	156
MS.gene065061.t1	MTR_5g006320	31.757	148	96	3	6	153	1	143	1.86e-19	79.7
MS.gene065061.t1	MTR_4g098490	39.535	86	52	0	69	154	74	159	1.19e-18	78.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065061.t1	AT2G27510	60.000	155	56	3	6	154	1	155	2.91e-60	184
MS.gene065061.t1	AT1G60950	56.209	153	56	5	6	153	1	147	7.46e-50	157
MS.gene065061.t1	AT1G10960	58.915	129	47	3	30	153	20	147	6.59e-47	149
MS.gene065061.t1	AT5G10000	52.381	126	57	2	31	153	22	147	8.26e-40	131
MS.gene065061.t1	AT4G14890	44.554	101	53	3	53	153	47	144	6.33e-20	81.3
MS.gene065061.t1	AT1G32550	39.535	86	52	0	69	154	69	154	3.45e-18	77.4
MS.gene065061.t1	AT1G32550	37.255	102	45	3	69	154	69	167	8.19e-13	63.5

Find 33 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAATCACAGTATCTGATTT+TGG	0.126493	1.2:+82889014	None:intergenic
TTCACAGATCTCAAACTTGA+TGG	0.324898	1.2:+82889335	None:intergenic
TTGTGCTGGCCAAGTGGTTA+CGG	0.329557	1.2:-82889117	MS.gene065061:CDS
CCCTGATGACTCTTACATTC+TGG	0.354348	1.2:-82889201	MS.gene065061:CDS
GCACCCGCTCTGCATGAATA+AGG	0.356204	1.2:+82889149	None:intergenic
TGTGCTGGCCAAGTGGTTAC+GGG	0.411836	1.2:-82889116	MS.gene065061:CDS
AAGAATGTGTCCAAAACATT+TGG	0.421132	1.2:-82889314	MS.gene065061:CDS
AATTCGTTCTCTGTTCCATC+TGG	0.449513	1.2:+82889230	None:intergenic
TCCAGAATGTAAGAGTCATC+AGG	0.451621	1.2:+82889200	None:intergenic
CTTTCAACAGCTTCATTGTA+CGG	0.469663	1.2:-82889398	MS.gene065061:CDS
CTGGATTCAGCTGAAGATGC+TGG	0.504908	1.2:-82889182	MS.gene065061:CDS
GGTGCATGTTCTACTTGTGC+TGG	0.505031	1.2:-82889131	MS.gene065061:CDS
GTATACAAAGTGAAACTGAT+TGG	0.509553	1.2:-82889254	MS.gene065061:CDS
CTACCTTATTCATGCAGAGC+GGG	0.513286	1.2:-82889152	MS.gene065061:CDS
CGACAGAACCCGTAACCACT+TGG	0.534235	1.2:+82889108	None:intergenic
GTGAAACTGATTGGACCAGA+TGG	0.549003	1.2:-82889245	MS.gene065061:CDS
TGTACGGAACAGTGCCCTCT+CGG	0.555453	1.2:-82889382	MS.gene065061:CDS
CGATGAGCAGCAAATCGAGA+AGG	0.589494	1.2:-82889063	MS.gene065061:CDS
CCAGAATGTAAGAGTCATCA+GGG	0.593554	1.2:+82889201	None:intergenic
TCTCACGGTGGACATATTCA+CGG	0.595716	1.2:+82889421	None:intergenic
ATCATTTAGAGTATCTGCAA+TGG	0.596293	1.2:-82889279	MS.gene065061:CDS
TTCTACTTGTGCTGGCCAAG+TGG	0.605670	1.2:-82889123	MS.gene065061:CDS
TTCAGCTGAAGATGCTGGAG+TGG	0.623617	1.2:-82889177	MS.gene065061:CDS
GATGAGCAGCAAATCGAGAA+GGG	0.629753	1.2:-82889062	MS.gene065061:CDS
TACTGTGATTTACACACACA+AGG	0.647153	1.2:-82889003	MS.gene065061:CDS
TCTCGATTTGCTGCTCATCG+AGG	0.652018	1.2:+82889066	None:intergenic
TGAAGCTGTTGAAAGTCTCA+CGG	0.659563	1.2:+82889406	None:intergenic
AGAGCACAAGATGTCCGAGA+GGG	0.687025	1.2:+82889368	None:intergenic
GCTACCTTATTCATGCAGAG+CGG	0.717700	1.2:-82889153	MS.gene065061:CDS
TGTGATTTACACACACAAGG+AGG	0.721823	1.2:-82889000	MS.gene065061:CDS
CAGAGCACAAGATGTCCGAG+AGG	0.730685	1.2:+82889367	None:intergenic
CAGAATGTAAGAGTCATCAG+GGG	0.754480	1.2:+82889202	None:intergenic
AGCTGTTGAAAGTCTCACGG+TGG	0.759008	1.2:+82889409	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATCACAGTATCTGATTTT+GGG	+	chr1.2:82889402-82889421	None:intergenic	25.0%
!!	TAAATCACAGTATCTGATTT+TGG	+	chr1.2:82889403-82889422	None:intergenic	25.0%
!!!	AGATTTTAGTCCAAATGTTT+TGG	+	chr1.2:82889113-82889132	None:intergenic	25.0%
	AAGAATGTGTCCAAAACATT+TGG	-	chr1.2:82889100-82889119	MS.gene065061:CDS	30.0%
	GTATACAAAGTGAAACTGAT+TGG	-	chr1.2:82889160-82889179	MS.gene065061:CDS	30.0%
!	ATCATTTAGAGTATCTGCAA+TGG	-	chr1.2:82889135-82889154	MS.gene065061:CDS	30.0%
	CTTTCAACAGCTTCATTGTA+CGG	-	chr1.2:82889016-82889035	MS.gene065061:CDS	35.0%
	TACTGTGATTTACACACACA+AGG	-	chr1.2:82889411-82889430	MS.gene065061:CDS	35.0%
	TTCACAGATCTCAAACTTGA+TGG	+	chr1.2:82889082-82889101	None:intergenic	35.0%
	AATTCGTTCTCTGTTCCATC+TGG	+	chr1.2:82889187-82889206	None:intergenic	40.0%
	CAGAATGTAAGAGTCATCAG+GGG	+	chr1.2:82889215-82889234	None:intergenic	40.0%
	CCAGAATGTAAGAGTCATCA+GGG	+	chr1.2:82889216-82889235	None:intergenic	40.0%
	TCCAGAATGTAAGAGTCATC+AGG	+	chr1.2:82889217-82889236	None:intergenic	40.0%
	TGAAGCTGTTGAAAGTCTCA+CGG	+	chr1.2:82889011-82889030	None:intergenic	40.0%
	TGTGATTTACACACACAAGG+AGG	-	chr1.2:82889414-82889433	MS.gene065061:CDS	40.0%
	CCCTGATGACTCTTACATTC+TGG	-	chr1.2:82889213-82889232	MS.gene065061:CDS	45.0%
	CTACCTTATTCATGCAGAGC+GGG	-	chr1.2:82889262-82889281	MS.gene065061:CDS	45.0%
	GATGAGCAGCAAATCGAGAA+GGG	-	chr1.2:82889352-82889371	MS.gene065061:CDS	45.0%
	GCTACCTTATTCATGCAGAG+CGG	-	chr1.2:82889261-82889280	MS.gene065061:CDS	45.0%
	GTGAAACTGATTGGACCAGA+TGG	-	chr1.2:82889169-82889188	MS.gene065061:CDS	45.0%
	TCTCACGGTGGACATATTCA+CGG	+	chr1.2:82888996-82889015	None:intergenic	45.0%
	AGAGCACAAGATGTCCGAGA+GGG	+	chr1.2:82889049-82889068	None:intergenic	50.0%
	AGCTGTTGAAAGTCTCACGG+TGG	+	chr1.2:82889008-82889027	None:intergenic	50.0%
	CGATGAGCAGCAAATCGAGA+AGG	-	chr1.2:82889351-82889370	MS.gene065061:CDS	50.0%
	CTGGATTCAGCTGAAGATGC+TGG	-	chr1.2:82889232-82889251	MS.gene065061:CDS	50.0%
	GGTGCATGTTCTACTTGTGC+TGG	-	chr1.2:82889283-82889302	MS.gene065061:CDS	50.0%
	TTCAGCTGAAGATGCTGGAG+TGG	-	chr1.2:82889237-82889256	MS.gene065061:CDS	50.0%
!	TTCTACTTGTGCTGGCCAAG+TGG	-	chr1.2:82889291-82889310	MS.gene065061:CDS	50.0%
!	TTGTGCTGGCCAAGTGGTTA+CGG	-	chr1.2:82889297-82889316	MS.gene065061:CDS	50.0%
!!	TCTCGATTTGCTGCTCATCG+AGG	+	chr1.2:82889351-82889370	None:intergenic	50.0%
	CAGAGCACAAGATGTCCGAG+AGG	+	chr1.2:82889050-82889069	None:intergenic	55.0%
	CGACAGAACCCGTAACCACT+TGG	+	chr1.2:82889309-82889328	None:intergenic	55.0%
	GCACCCGCTCTGCATGAATA+AGG	+	chr1.2:82889268-82889287	None:intergenic	55.0%
	TGTACGGAACAGTGCCCTCT+CGG	-	chr1.2:82889032-82889051	MS.gene065061:CDS	55.0%
!	TGTGCTGGCCAAGTGGTTAC+GGG	-	chr1.2:82889298-82889317	MS.gene065061:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	82888986	82889450	82888986	ID=MS.gene065061
chr1.2	mRNA	82888986	82889450	82888986	ID=MS.gene065061.t1;Parent=MS.gene065061
chr1.2	exon	82888986	82889450	82888986	ID=MS.gene065061.t1.exon1;Parent=MS.gene065061.t1
chr1.2	CDS	82888986	82889450	82888986	ID=cds.MS.gene065061.t1;Parent=MS.gene065061.t1

Gene Sequence

>MS.gene065061

ATGTCAGCCGTGAATATGTCCACCGTGAGACTTTCAACAGCTTCATTGTACGGAACAGTGCCCTCTCGGACATCTTGTGCTCTGATGAAGAGTCCATCAAGTTTGAGATCTGTGAAGAATGTGTCCAAAACATTTGGACTAAAATCTTCATCATTTAGAGTATCTGCAATGGCAGTATACAAAGTGAAACTGATTGGACCAGATGGAACAGAGAACGAATTTGATGCCCCTGATGACTCTTACATTCTGGATTCAGCTGAAGATGCTGGAGTGGAGCTACCTTATTCATGCAGAGCGGGTGCATGTTCTACTTGTGCTGGCCAAGTGGTTACGGGTTCTGTCGATCAGTCTGATCAATCTTTCCTCGATGAGCAGCAAATCGAGAAGGGTTATCTTCTCACTTGTGTCTCATACCCAAAATCAGATACTGTGATTTACACACACAAGGAGGAAGAACTCTACTAA

Protein sequence

>MS.gene065061.t1

MSAVNMSTVRLSTASLYGTVPSRTSCALMKSPSSLRSVKNVSKTFGLKSSSFRVSAMAVYKVKLIGPDGTENEFDAPDDSYILDSAEDAGVELPYSCRAGACSTCAGQVVTGSVDQSDQSFLDEQQIEKGYLLTCVSYPKSDTVIYTHKEEELY