AlfalfaGEDB | MS.gene067740

M. sativa cultivar XinJiangDaYe / MS.gene067740

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067740.t1	XP_003606105.2	98.1	155	3	0	1	155	1	155	1.60E-78	302

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067740.t1	P27788	61.8	152	58	0	4	155	1	152	1.8e-46	186.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067740.t1	G7JHA6	98.1	155	3	0	1	155	1	155	1.2e-78	302.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051024	MS.gene067740	0.835724	1.42E-56	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene067740	MS.gene030942	PPI
MS.gene067740	MS.gene21845	PPI
MS.gene67232	MS.gene067740	PPI
MS.gene067740	MS.gene024872	PPI
MS.gene067740	MS.gene000152	PPI
MS.gene47889	MS.gene067740	PPI
MS.gene27932	MS.gene067740	PPI
MS.gene92294	MS.gene067740	PPI
MS.gene067740	MS.gene030944	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067740.t1	MTR_4g053120	98.065	155	3	0	1	155	1	155	2.72e-111	312
MS.gene067740.t1	MTR_2g086510	73.548	155	41	0	1	155	1	155	1.57e-82	240
MS.gene067740.t1	MTR_1g112030	65.385	156	48	3	4	155	7	160	4.14e-66	198
MS.gene067740.t1	MTR_1g112030	65.385	156	48	3	4	155	3	156	4.57e-66	198
MS.gene067740.t1	MTR_1g112050	63.871	155	50	3	4	154	1	153	3.41e-63	191
MS.gene067740.t1	MTR_2g006290	60.000	135	39	4	24	154	25	148	4.96e-51	160
MS.gene067740.t1	MTR_8g088720	64.220	109	38	1	46	154	39	146	7.44e-47	149
MS.gene067740.t1	MTR_5g006320	34.437	151	91	4	4	154	1	143	2.28e-21	84.7
MS.gene067740.t1	MTR_4g098490	29.452	146	100	1	13	155	14	159	7.65e-18	76.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene067740.t1	AT2G27510	60.000	155	59	2	4	155	1	155	3.44e-62	188
MS.gene067740.t1	AT1G60950	66.667	111	34	2	46	154	38	147	3.32e-48	153
MS.gene067740.t1	AT1G10960	58.209	134	48	4	20	151	17	144	8.23e-47	149
MS.gene067740.t1	AT5G10000	52.475	101	48	0	54	154	47	147	1.04e-37	126
MS.gene067740.t1	AT4G14890	46.316	95	49	2	60	154	52	144	2.45e-22	87.4
MS.gene067740.t1	AT1G32550	38.372	86	53	0	70	155	69	154	7.39e-18	76.3
MS.gene067740.t1	AT1G32550	35.294	102	47	3	70	155	69	167	4.41e-12	61.6

Find 42 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGAAGTTCCCTTCATCTTTC+GGG	0.151505	4.2:+49252037	MS.gene067740:CDS
TGTGCTGGAAAGATTGTTTC+TGG	0.213905	4.2:+49252267	MS.gene067740:CDS
GCTCCAGCACGGCATGAATA+AGG	0.234304	4.2:-49252234	None:intergenic
GCTTACAAGGTCAAGCTTAT+TGG	0.250602	4.2:+49252129	MS.gene067740:CDS
GCGAAGTTCCCTTCATCTTT+CGG	0.263582	4.2:+49252036	MS.gene067740:CDS
TCAATCTGATGGCTCTTTCT+TGG	0.285813	4.2:+49252299	MS.gene067740:CDS
TGACTTCAAGCCACAAGATT+TGG	0.293119	4.2:-49252076	None:intergenic
CTTGGATGATAATCAACTTA+AGG	0.311771	4.2:+49252317	MS.gene067740:CDS
AACTGCAATGGCTGCTTACA+AGG	0.410013	4.2:+49252116	MS.gene067740:CDS
CTCCCTTATTCATGCCGTGC+TGG	0.418450	4.2:+49252231	MS.gene067740:CDS
TCTAAGATGTAAACATCGTC+GGG	0.429231	4.2:-49252183	None:intergenic
GGAGCTTGCTCCACTTGTGC+TGG	0.438234	4.2:+49252252	MS.gene067740:CDS
CTCCAGCACGGCATGAATAA+GGG	0.444632	4.2:-49252233	None:intergenic
CAGTTTGAATCATGCACAGC+GGG	0.458520	4.2:-49251981	None:intergenic
TGGAAAGATTGTTTCTGGCT+CGG	0.490169	4.2:+49252272	MS.gene067740:CDS
TATGCAATCTGCGGTTGGAT+AGG	0.494449	4.2:-49252364	None:intergenic
AACAATCTTTCCAGCACAAG+TGG	0.505407	4.2:-49252262	None:intergenic
GTCAAGCTTATTGGACCAGA+TGG	0.514160	4.2:+49252138	MS.gene067740:CDS
TTTGTAGACCCGAAAGATGA+AGG	0.518911	4.2:-49252045	None:intergenic
ATCTAAGATGTAAACATCGT+CGG	0.538845	4.2:-49252184	None:intergenic
GGCTCGGTGGATCAATCTGA+TGG	0.542825	4.2:+49252288	MS.gene067740:CDS
AAGAGTGTTTCCAAATCTTG+TGG	0.543692	4.2:+49252066	MS.gene067740:CDS
GATGATAATCAACTTAAGGA+TGG	0.547500	4.2:+49252321	MS.gene067740:CDS
TCAATTATGCAATCTGCGGT+TGG	0.549164	4.2:-49252369	None:intergenic
TTAGATGCAGCAGAAGATGC+CGG	0.586722	4.2:+49252201	MS.gene067740:CDS
TAGCAATGGTTGATTGATTG+CGG	0.600774	4.2:-49252008	None:intergenic
TTCATATAGAACAACTGCAA+TGG	0.601127	4.2:+49252104	MS.gene067740:CDS
GAACTTCGCTGTGGTAGCAA+TGG	0.602045	4.2:-49252022	None:intergenic
TGAATAAGGGAGTTCAACTC+CGG	0.604511	4.2:-49252220	None:intergenic
TTGTAGACCCGAAAGATGAA+GGG	0.611828	4.2:-49252044	None:intergenic
AAGTGGAGCAAGCTCCAGCA+CGG	0.615367	4.2:-49252245	None:intergenic
AAAGATTGTTTCTGGCTCGG+TGG	0.616952	4.2:+49252275	MS.gene067740:CDS
ATAATTGAAACTCACAAGGA+AGG	0.627357	4.2:+49252384	MS.gene067740:CDS
GGAAACATTCACAGCTGACA+AGG	0.632858	4.2:-49251959	None:intergenic
TGCGGTTGGATAGGAAACAC+AGG	0.661542	4.2:-49252355	None:intergenic
AGTTTCAATTATGCAATCTG+CGG	0.680241	4.2:-49252373	None:intergenic
TTGCATAATTGAAACTCACA+AGG	0.686967	4.2:+49252380	MS.gene067740:CDS
CTAAGATGTAAACATCGTCG+GGG	0.693590	4.2:-49252182	None:intergenic
TAAGATGTAAACATCGTCGG+GGG	0.711580	4.2:-49252181	None:intergenic
GCAGTTTGAATCATGCACAG+CGG	0.732615	4.2:-49251982	None:intergenic
AGATGAAGGGAACTTCGCTG+TGG	0.735830	4.2:-49252031	None:intergenic
AGTTTGAATCATGCACAGCG+GGG	0.756151	4.2:-49251980	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGTTTCAATTATGCAATCTG+CGG	-	chr4.2:49252376-49252395	None:intergenic	30.0%
	ATAATTGAAACTCACAAGGA+AGG	+	chr4.2:49252384-49252403	MS.gene067740:CDS	30.0%
	CTTGGATGATAATCAACTTA+AGG	+	chr4.2:49252317-49252336	MS.gene067740:CDS	30.0%
	GATGATAATCAACTTAAGGA+TGG	+	chr4.2:49252321-49252340	MS.gene067740:CDS	30.0%
	TTCATATAGAACAACTGCAA+TGG	+	chr4.2:49252104-49252123	MS.gene067740:CDS	30.0%
	TTGCATAATTGAAACTCACA+AGG	+	chr4.2:49252380-49252399	MS.gene067740:CDS	30.0%
!	AACTCATTTTCTTTTCCATC+TGG	-	chr4.2:49252156-49252175	None:intergenic	30.0%
!	ATCTAAGATGTAAACATCGT+CGG	-	chr4.2:49252187-49252206	None:intergenic	30.0%
	TAGCAATGGTTGATTGATTG+CGG	-	chr4.2:49252011-49252030	None:intergenic	35.0%
!	TCTAAGATGTAAACATCGTC+GGG	-	chr4.2:49252186-49252205	None:intergenic	35.0%
!!	AAGAGTGTTTCCAAATCTTG+TGG	+	chr4.2:49252066-49252085	MS.gene067740:CDS	35.0%
	AACAATCTTTCCAGCACAAG+TGG	-	chr4.2:49252265-49252284	None:intergenic	40.0%
	CTAAGATGTAAACATCGTCG+GGG	-	chr4.2:49252185-49252204	None:intergenic	40.0%
	GCTTACAAGGTCAAGCTTAT+TGG	+	chr4.2:49252129-49252148	MS.gene067740:CDS	40.0%
	TAAGATGTAAACATCGTCGG+GGG	-	chr4.2:49252184-49252203	None:intergenic	40.0%
	TCAATCTGATGGCTCTTTCT+TGG	+	chr4.2:49252299-49252318	MS.gene067740:CDS	40.0%
	TCAATTATGCAATCTGCGGT+TGG	-	chr4.2:49252372-49252391	None:intergenic	40.0%
	TGAATAAGGGAGTTCAACTC+CGG	-	chr4.2:49252223-49252242	None:intergenic	40.0%
	TGACTTCAAGCCACAAGATT+TGG	-	chr4.2:49252079-49252098	None:intergenic	40.0%
	TGTGCTGGAAAGATTGTTTC+TGG	+	chr4.2:49252267-49252286	MS.gene067740:CDS	40.0%
	TTGTAGACCCGAAAGATGAA+GGG	-	chr4.2:49252047-49252066	None:intergenic	40.0%
	TTTGTAGACCCGAAAGATGA+AGG	-	chr4.2:49252048-49252067	None:intergenic	40.0%
!!	TGGAAAGATTGTTTCTGGCT+CGG	+	chr4.2:49252272-49252291	MS.gene067740:CDS	40.0%
	AACTGCAATGGCTGCTTACA+AGG	+	chr4.2:49252116-49252135	MS.gene067740:CDS	45.0%
	AGTTTGAATCATGCACAGCG+GGG	-	chr4.2:49251983-49252002	None:intergenic	45.0%
	CAGTTTGAATCATGCACAGC+GGG	-	chr4.2:49251984-49252003	None:intergenic	45.0%
	CGAAGTTCCCTTCATCTTTC+GGG	+	chr4.2:49252037-49252056	MS.gene067740:CDS	45.0%
	GCAGTTTGAATCATGCACAG+CGG	-	chr4.2:49251985-49252004	None:intergenic	45.0%
	GCGAAGTTCCCTTCATCTTT+CGG	+	chr4.2:49252036-49252055	MS.gene067740:CDS	45.0%
	GGAAACATTCACAGCTGACA+AGG	-	chr4.2:49251962-49251981	None:intergenic	45.0%
	GTCAAGCTTATTGGACCAGA+TGG	+	chr4.2:49252138-49252157	MS.gene067740:CDS	45.0%
	TATGCAATCTGCGGTTGGAT+AGG	-	chr4.2:49252367-49252386	None:intergenic	45.0%
	TTAGATGCAGCAGAAGATGC+CGG	+	chr4.2:49252201-49252220	MS.gene067740:CDS	45.0%
!!	AAAGATTGTTTCTGGCTCGG+TGG	+	chr4.2:49252275-49252294	MS.gene067740:CDS	45.0%
	AGATGAAGGGAACTTCGCTG+TGG	-	chr4.2:49252034-49252053	None:intergenic	50.0%
	CTCCAGCACGGCATGAATAA+GGG	-	chr4.2:49252236-49252255	None:intergenic	50.0%
	GAACTTCGCTGTGGTAGCAA+TGG	-	chr4.2:49252025-49252044	None:intergenic	50.0%
	TGCGGTTGGATAGGAAACAC+AGG	-	chr4.2:49252358-49252377	None:intergenic	50.0%
	AAGTGGAGCAAGCTCCAGCA+CGG	-	chr4.2:49252248-49252267	None:intergenic	55.0%
	CTCCCTTATTCATGCCGTGC+TGG	+	chr4.2:49252231-49252250	MS.gene067740:CDS	55.0%
	GCTCCAGCACGGCATGAATA+AGG	-	chr4.2:49252237-49252256	None:intergenic	55.0%
!!	GGCTCGGTGGATCAATCTGA+TGG	+	chr4.2:49252288-49252307	MS.gene067740:CDS	55.0%
!	GGAGCTTGCTCCACTTGTGC+TGG	+	chr4.2:49252252-49252271	MS.gene067740:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	49251952	49252419	49251952	ID=MS.gene067740
chr4.2	mRNA	49251952	49252419	49251952	ID=MS.gene067740.t1;Parent=MS.gene067740
chr4.2	exon	49251952	49252419	49251952	ID=MS.gene067740.t1.exon1;Parent=MS.gene067740.t1
chr4.2	CDS	49251952	49252419	49251952	ID=cds.MS.gene067740.t1;Parent=MS.gene067740.t1

Gene Sequence

>MS.gene067740

ATGGCATCCTTGTCAGCTGTGAATGTTTCCCCGCTGTGCATGATTCAAACTGCAAACCGCAATCAATCAACCATTGCTACCACAGCGAAGTTCCCTTCATCTTTCGGGTCTACAAAGAGTGTTTCCAAATCTTGTGGCTTGAAGTCATCATCTTCATATAGAACAACTGCAATGGCTGCTTACAAGGTCAAGCTTATTGGACCAGATGGAAAAGAAAATGAGTTTGACGCCCCCGACGATGTTTACATCTTAGATGCAGCAGAAGATGCCGGAGTTGAACTCCCTTATTCATGCCGTGCTGGAGCTTGCTCCACTTGTGCTGGAAAGATTGTTTCTGGCTCGGTGGATCAATCTGATGGCTCTTTCTTGGATGATAATCAACTTAAGGATGGCTTTGTGCTTACCTGTGTTTCCTATCCAACCGCAGATTGCATAATTGAAACTCACAAGGAAGGCGAACTCTACTGA

Protein sequence

>MS.gene067740.t1

MASLSAVNVSPLCMIQTANRNQSTIATTAKFPSSFGSTKSVSKSCGLKSSSSYRTTAMAAYKVKLIGPDGKENEFDAPDDVYILDAAEDAGVELPYSCRAGACSTCAGKIVSGSVDQSDGSFLDDNQLKDGFVLTCVSYPTADCIIETHKEGELY