AlfalfaGEDB | MS.gene024872

M. sativa cultivar XinJiangDaYe / MS.gene024872

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024872.t1	XP_003610722.1	94.8	154	4	2	1	151	1	153	1.40E-74	288.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024872.t1	O23344	81.1	106	20	0	46	151	49	154	1.4e-46	187.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024872.t1	G7K3M7	94.8	154	4	2	1	151	1	153	9.9e-75	288.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene47889	MS.gene024872	PPI
MS.gene27932	MS.gene024872	PPI
MS.gene84322	MS.gene024872	PPI
MS.gene065061	MS.gene024872	PPI
MS.gene030944	MS.gene024872	PPI
MS.gene034453	MS.gene024872	PPI
MS.gene067740	MS.gene024872	PPI
MS.gene024872	MS.gene88235	PPI
MS.gene024872	MS.gene35417	PPI
MS.gene024872	MS.gene66754	PPI
MS.gene74977	MS.gene024872	PPI
MS.gene67232	MS.gene024872	PPI
MS.gene024872	MS.gene03847	PPI
MS.gene024872	MS.gene029328	PPI
MS.gene030942	MS.gene024872	PPI
MS.gene024872	MS.gene43037	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024872.t1	MTR_5g006320	94.805	154	4	2	1	151	1	153	2.58e-103	292
MS.gene024872.t1	MTR_2g086510	34.416	154	83	4	7	142	2	155	6.62e-23	88.6
MS.gene024872.t1	MTR_4g053120	33.113	151	91	4	1	141	4	154	2.57e-22	87.0
MS.gene024872.t1	MTR_1g112050	37.302	126	72	4	22	141	29	153	3.72e-19	79.0
MS.gene024872.t1	MTR_1g112030	38.655	119	62	3	34	141	37	155	4.15e-19	79.0
MS.gene024872.t1	MTR_1g112030	38.655	119	62	3	34	141	41	159	4.86e-19	79.0
MS.gene024872.t1	MTR_2g006290	31.387	137	86	3	10	141	15	148	2.59e-18	76.6
MS.gene024872.t1	MTR_8g088720	34.400	125	77	2	20	143	28	148	7.77e-18	75.5
MS.gene024872.t1	MTR_4g098490	32.759	116	71	3	33	147	55	164	2.88e-14	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene024872.t1	AT4G14890	81.132	106	20	0	46	151	49	154	5.44e-62	187
MS.gene024872.t1	AT2G27510	34.395	157	86	5	1	142	1	155	4.27e-23	89.4
MS.gene024872.t1	AT1G60950	34.532	139	81	6	10	142	14	148	2.73e-18	76.6
MS.gene024872.t1	AT5G10000	32.847	137	82	3	16	142	12	148	1.65e-16	72.0
MS.gene024872.t1	AT1G10960	30.435	138	88	4	10	142	14	148	4.84e-15	68.2
MS.gene024872.t1	AT1G32550	31.915	141	88	4	9	147	25	159	4.67e-14	66.2

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCCAATGCCTTTGATAATA+TGG	0.193692	5.4:+2130018	None:intergenic
TCGTTCTGATTGTCATATTA+AGG	0.194811	5.4:-2129846	MS.gene024872:CDS
TTCTGTCTGAAGAGAGTTAA+GGG	0.376635	5.4:+2130205	None:intergenic
TTTGATAATATGGTTTCATC+AGG	0.398760	5.4:+2130028	None:intergenic
CTGAAAGCTAAATTAGTCAT+TGG	0.415804	5.4:+2129781	None:intergenic
CTTCTGTCTGAAGAGAGTTA+AGG	0.435022	5.4:+2130204	None:intergenic
AGGATAGGGGAGCTTAGTTG+TGG	0.462415	5.4:+2130174	None:intergenic
GAGTTAAGGGTGTAAATTGA+AGG	0.462964	5.4:+2130218	None:intergenic
TTATCAAAGGCATTGGACTC+TGG	0.464183	5.4:-2130013	MS.gene024872:CDS
GGATAGGGGAGCTTAGTTGT+GGG	0.466042	5.4:+2130175	None:intergenic
AACCATATTATCAAAGGCAT+TGG	0.476989	5.4:-2130020	MS.gene024872:CDS
TGTGTTGAGCTGAGAAGGAT+AGG	0.480320	5.4:+2130159	None:intergenic
ACAAGACCACGCCTTGGTTC+AGG	0.487317	5.4:-2130139	MS.gene024872:CDS
TGTGAAGTCTTCTTATAAAG+TGG	0.499881	5.4:-2130092	MS.gene024872:CDS
ACAATCAGAACGAGGGTAAG+AGG	0.509900	5.4:+2129856	None:intergenic
GTGCCTCATGACTGTAAGCT+TGG	0.510320	5.4:-2129983	MS.gene024872:CDS
AGTTAAGGGTGTAAATTGAA+GGG	0.533055	5.4:+2130219	None:intergenic
GTGTTGAGCTGAGAAGGATA+GGG	0.540357	5.4:+2130160	None:intergenic
TAAAGTGGTGATTGAGCATG+AGG	0.567310	5.4:-2130077	MS.gene024872:CDS
TGATGAAACCATATTATCAA+AGG	0.569530	5.4:-2130026	MS.gene024872:CDS
GGTCTTGTGTTGAGCTGAGA+AGG	0.574174	5.4:+2130154	None:intergenic
CTCAACACAAGACCACGCCT+TGG	0.584774	5.4:-2130145	MS.gene024872:CDS
GTGCCACTGATAAGACGAGC+AGG	0.599960	5.4:+2129944	None:intergenic
TCATATTAAGGTTATAGCAG+AGG	0.608535	5.4:-2129834	MS.gene024872:CDS
TCACAGTGAAAGTGAGTGAT+GGG	0.611048	5.4:+2130110	None:intergenic
GGCACAGTTGATCAGAGTGA+TGG	0.619808	5.4:-2129926	MS.gene024872:CDS
TTCACAGTGAAAGTGAGTGA+TGG	0.627712	5.4:+2130109	None:intergenic
TGTTGAGCTGAGAAGGATAG+GGG	0.635174	5.4:+2130161	None:intergenic
TGTCCTGCTCGTCTTATCAG+TGG	0.649957	5.4:-2129947	MS.gene024872:CDS
AAGCTTACAGTCATGAGGCA+CGG	0.652590	5.4:+2129985	None:intergenic
GATAGGGGAGCTTAGTTGTG+GGG	0.665612	5.4:+2130176	None:intergenic
AGTGATGATGTTGTAGAGCG+TGG	0.677618	5.4:-2129896	MS.gene024872:CDS
ACTCCAAGCTTACAGTCATG+AGG	0.678735	5.4:+2129980	None:intergenic
TAATATGACAATCAGAACGA+GGG	0.699912	5.4:+2129849	None:intergenic
AAAGTGGTGATTGAGCATGA+GGG	0.724790	5.4:-2130076	MS.gene024872:CDS
TGTGAACCTGAACCAAGGCG+TGG	0.725548	5.4:+2130133	None:intergenic
TTAATATGACAATCAGAACG+AGG	0.733351	5.4:+2129848	None:intergenic
ATGGGTGTGAACCTGAACCA+AGG	0.761073	5.4:+2130128	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGATGAAACCATATTATCAA+AGG	-	chr5.4:2129993-2130012	MS.gene024872:CDS	25.0%
!!	TTTGATAATATGGTTTCATC+AGG	+	chr5.4:2129994-2130013	None:intergenic	25.0%
	TAATATGACAATCAGAACGA+GGG	+	chr5.4:2130173-2130192	None:intergenic	30.0%
	TCATATTAAGGTTATAGCAG+AGG	-	chr5.4:2130185-2130204	MS.gene024872:CDS	30.0%
	TCGTTCTGATTGTCATATTA+AGG	-	chr5.4:2130173-2130192	MS.gene024872:CDS	30.0%
	TGTGAAGTCTTCTTATAAAG+TGG	-	chr5.4:2129927-2129946	MS.gene024872:CDS	30.0%
	TTAATATGACAATCAGAACG+AGG	+	chr5.4:2130174-2130193	None:intergenic	30.0%
!	AACCATATTATCAAAGGCAT+TGG	-	chr5.4:2129999-2130018	MS.gene024872:CDS	30.0%
!	AGTTAAGGGTGTAAATTGAA+GGG	+	chr5.4:2129803-2129822	None:intergenic	30.0%
	TTCTGTCTGAAGAGAGTTAA+GGG	+	chr5.4:2129817-2129836	None:intergenic	35.0%
!	GAGTTAAGGGTGTAAATTGA+AGG	+	chr5.4:2129804-2129823	None:intergenic	35.0%
!	GTCCAATGCCTTTGATAATA+TGG	+	chr5.4:2130004-2130023	None:intergenic	35.0%
	AAAGTGGTGATTGAGCATGA+GGG	-	chr5.4:2129943-2129962	MS.gene024872:CDS	40.0%
	CTTCTGTCTGAAGAGAGTTA+AGG	+	chr5.4:2129818-2129837	None:intergenic	40.0%
	TAAAGTGGTGATTGAGCATG+AGG	-	chr5.4:2129942-2129961	MS.gene024872:CDS	40.0%
	TCACAGTGAAAGTGAGTGAT+GGG	+	chr5.4:2129912-2129931	None:intergenic	40.0%
	TTCACAGTGAAAGTGAGTGA+TGG	+	chr5.4:2129913-2129932	None:intergenic	40.0%
!!	TTATCAAAGGCATTGGACTC+TGG	-	chr5.4:2130006-2130025	MS.gene024872:CDS	40.0%
	AAGCTTACAGTCATGAGGCA+CGG	+	chr5.4:2130037-2130056	None:intergenic	45.0%
	ACAATCAGAACGAGGGTAAG+AGG	+	chr5.4:2130166-2130185	None:intergenic	45.0%
	ACTCCAAGCTTACAGTCATG+AGG	+	chr5.4:2130042-2130061	None:intergenic	45.0%
	GTGTTGAGCTGAGAAGGATA+GGG	+	chr5.4:2129862-2129881	None:intergenic	45.0%
	TGTGTTGAGCTGAGAAGGAT+AGG	+	chr5.4:2129863-2129882	None:intergenic	45.0%
	TGTTGAGCTGAGAAGGATAG+GGG	+	chr5.4:2129861-2129880	None:intergenic	45.0%
!	AGTGATGATGTTGTAGAGCG+TGG	-	chr5.4:2130123-2130142	MS.gene024872:CDS	45.0%
	AGGATAGGGGAGCTTAGTTG+TGG	+	chr5.4:2129848-2129867	None:intergenic	50.0%
	ATGGGTGTGAACCTGAACCA+AGG	+	chr5.4:2129894-2129913	None:intergenic	50.0%
	GATAGGGGAGCTTAGTTGTG+GGG	+	chr5.4:2129846-2129865	None:intergenic	50.0%
	GGATAGGGGAGCTTAGTTGT+GGG	+	chr5.4:2129847-2129866	None:intergenic	50.0%
	GGCACAGTTGATCAGAGTGA+TGG	-	chr5.4:2130093-2130112	MS.gene024872:CDS	50.0%
	GTGCCTCATGACTGTAAGCT+TGG	-	chr5.4:2130036-2130055	MS.gene024872:CDS	50.0%
	TGTCCTGCTCGTCTTATCAG+TGG	-	chr5.4:2130072-2130091	MS.gene024872:CDS	50.0%
!	GGTCTTGTGTTGAGCTGAGA+AGG	+	chr5.4:2129868-2129887	None:intergenic	50.0%
	ACAAGACCACGCCTTGGTTC+AGG	-	chr5.4:2129880-2129899	MS.gene024872:CDS	55.0%
	CTCAACACAAGACCACGCCT+TGG	-	chr5.4:2129874-2129893	MS.gene024872:CDS	55.0%
	TGTGAACCTGAACCAAGGCG+TGG	+	chr5.4:2129889-2129908	None:intergenic	55.0%
!	GTGCCACTGATAAGACGAGC+AGG	+	chr5.4:2130078-2130097	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	2129793	2130248	2129793	ID=MS.gene024872
chr5.4	mRNA	2129793	2130248	2129793	ID=MS.gene024872.t1;Parent=MS.gene024872
chr5.4	exon	2129793	2130248	2129793	ID=MS.gene024872.t1.exon1;Parent=MS.gene024872.t1
chr5.4	CDS	2129793	2130248	2129793	ID=cds.MS.gene024872.t1;Parent=MS.gene024872.t1

Gene Sequence

>MS.gene024872

ATGGCAACCCTTCAATTTACACCCTTAACTCTCTTCAGACAGAAGAAGCACCCCACAACTAAGCTCCCCTATCCTTCTCAGCTCAACACAAGACCACGCCTTGGTTCAGGTTCACACCCATCACTCACTTTCACTGTGAAGTCTTCTTATAAAGTGGTGATTGAGCATGAGGGCAAGACTACACAGCTTGAAGTTGAACCTGATGAAACCATATTATCAAAGGCATTGGACTCTGGTTTGTCCGTGCCTCATGACTGTAAGCTTGGAGTTTGCATGACTTGTCCTGCTCGTCTTATCAGTGGCACAGTTGATCAGAGTGATGGTATGCTCAGTGATGATGTTGTAGAGCGTGGCTATGCACTCTTGTGTGCCTCTTACCCTCGTTCTGATTGTCATATTAAGGTTATAGCAGAGGATGAGCTACTCTCAATGCAGTTAGCTACAGCCAATGACTAA

Protein sequence

>MS.gene024872.t1

MATLQFTPLTLFRQKKHPTTKLPYPSQLNTRPRLGSGSHPSLTFTVKSSYKVVIEHEGKTTQLEVEPDETILSKALDSGLSVPHDCKLGVCMTCPARLISGTVDQSDGMLSDDVVERGYALLCASYPRSDCHIKVIAEDELLSMQLATAND