AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21845


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21845.t1 MTR_5g006320 96.078 153 3 1 1 150 1 153 1.38e-105 298
MS.gene21845.t1 MTR_2g086510 37.063 143 85 4 3 141 14 155 2.05e-22 87.4
MS.gene21845.t1 MTR_4g053120 33.113 151 90 4 1 140 4 154 3.75e-22 86.7
MS.gene21845.t1 MTR_1g112050 36.719 128 75 4 15 140 30 153 3.38e-19 79.0
MS.gene21845.t1 MTR_1g112030 37.815 119 63 3 33 140 37 155 8.90e-19 78.2
MS.gene21845.t1 MTR_1g112030 37.815 119 63 3 33 140 41 159 1.03e-18 77.8
MS.gene21845.t1 MTR_2g006290 33.333 135 82 3 11 140 17 148 1.23e-18 77.4
MS.gene21845.t1 MTR_8g088720 33.333 138 83 3 10 142 15 148 3.64e-18 76.3
MS.gene21845.t1 MTR_4g098490 32.759 116 71 3 32 146 55 164 2.50e-14 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21845.t1 AT4G14890 81.132 106 20 0 45 150 49 154 8.65e-62 187
MS.gene21845.t1 AT2G27510 33.758 157 86 5 1 141 1 155 1.51e-22 87.8
MS.gene21845.t1 AT1G60950 35.556 135 77 6 13 141 18 148 1.03e-17 75.1
MS.gene21845.t1 AT5G10000 31.387 137 84 2 15 141 12 148 2.60e-16 71.6
MS.gene21845.t1 AT1G10960 32.353 136 84 4 11 141 16 148 2.46e-15 68.9
MS.gene21845.t1 AT1G32550 31.915 141 87 4 9 146 25 159 3.35e-14 66.6

Find 40 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCAATGCCTTTGATAATA+TGG 0.193692 5.2:+1138370 None:intergenic
TCGTTCTGATTGTCATATTA+AGG 0.194811 5.2:-1138198 MS.gene21845:CDS
TTTGATAATATGGTTTCATC+AGG 0.398760 5.2:+1138380 None:intergenic
CTGAAAGCTAAATTAGTCAT+TGG 0.415804 5.2:+1138133 None:intergenic
TTCTGTCTGAAGAGAGTTAA+GGG 0.429353 5.2:+1138554 None:intergenic
AGGATAGGGGAGCTTAGTTG+TGG 0.462415 5.2:+1138526 None:intergenic
GAGTTAAGGGTGTAAATTGA+AGG 0.462964 5.2:+1138567 None:intergenic
TTATCAAAGGCATTGGACTC+TGG 0.464183 5.2:-1138365 MS.gene21845:CDS
CTTCTGTCTGAAGAGAGTTA+AGG 0.465427 5.2:+1138553 None:intergenic
GGATAGGGGAGCTTAGTTGT+GGG 0.466042 5.2:+1138527 None:intergenic
AACCATATTATCAAAGGCAT+TGG 0.476989 5.2:-1138372 MS.gene21845:CDS
TGTGTTGAGCTGAGAAGGAT+AGG 0.480320 5.2:+1138511 None:intergenic
ACAAGACCACGCCTTGGTTC+AGG 0.487317 5.2:-1138491 MS.gene21845:CDS
ACAATCAGAACGAGGGTAAG+AGG 0.509900 5.2:+1138208 None:intergenic
GTGCCTCATGACTGTAAGCT+TGG 0.510320 5.2:-1138335 MS.gene21845:CDS
AGTTAAGGGTGTAAATTGAA+GGG 0.533055 5.2:+1138568 None:intergenic
GTGTTGAGCTGAGAAGGATA+GGG 0.540357 5.2:+1138512 None:intergenic
TGTGAGGTCTTCTTATAAAG+TGG 0.558437 5.2:-1138444 MS.gene21845:CDS
TAAAGTGGTGATTGAGCATG+AGG 0.567310 5.2:-1138429 MS.gene21845:CDS
TGATGAAACCATATTATCAA+AGG 0.569530 5.2:-1138378 MS.gene21845:CDS
GGTCTTGTGTTGAGCTGAGA+AGG 0.574174 5.2:+1138506 None:intergenic
CTCAACACAAGACCACGCCT+TGG 0.584774 5.2:-1138497 MS.gene21845:CDS
TCATATTAAGGTTATAGCAG+AGG 0.608535 5.2:-1138186 MS.gene21845:CDS
TCACAGTGAAAGTGAGTGAT+GGG 0.611048 5.2:+1138462 None:intergenic
GTGCCACTGATAAGACGAGC+GGG 0.615009 5.2:+1138296 None:intergenic
GGCACAGTTGATCAGAGTGA+TGG 0.619808 5.2:-1138278 MS.gene21845:CDS
TGTTGAGCTGAGAAGGATAG+GGG 0.635174 5.2:+1138513 None:intergenic
CTCACAGTGAAAGTGAGTGA+TGG 0.635887 5.2:+1138461 None:intergenic
AAGCTTACAGTCATGAGGCA+CGG 0.652590 5.2:+1138337 None:intergenic
TGTCCCGCTCGTCTTATCAG+TGG 0.665093 5.2:-1138299 MS.gene21845:CDS
GATAGGGGAGCTTAGTTGTG+GGG 0.665612 5.2:+1138528 None:intergenic
AGTGATGATGTTGTAGAGCG+TGG 0.677618 5.2:-1138248 MS.gene21845:CDS
ACTCCAAGCTTACAGTCATG+AGG 0.678735 5.2:+1138332 None:intergenic
TAATATGACAATCAGAACGA+GGG 0.699912 5.2:+1138201 None:intergenic
CATCACTCACTTTCACTGTG+AGG 0.704299 5.2:-1138460 MS.gene21845:CDS
TGTGCCACTGATAAGACGAG+CGG 0.724700 5.2:+1138295 None:intergenic
AAAGTGGTGATTGAGCATGA+GGG 0.724790 5.2:-1138428 MS.gene21845:CDS
TGTGAACCTGAACCAAGGCG+TGG 0.725548 5.2:+1138485 None:intergenic
TTAATATGACAATCAGAACG+AGG 0.733351 5.2:+1138200 None:intergenic
ATGGGTGTGAACCTGAACCA+AGG 0.761073 5.2:+1138480 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TGATGAAACCATATTATCAA+AGG - chr5.2:1138342-1138361 MS.gene21845:CDS 25.0%
!! TTTGATAATATGGTTTCATC+AGG + chr5.2:1138343-1138362 None:intergenic 25.0%
TAATATGACAATCAGAACGA+GGG + chr5.2:1138522-1138541 None:intergenic 30.0%
TCATATTAAGGTTATAGCAG+AGG - chr5.2:1138534-1138553 MS.gene21845:CDS 30.0%
TCGTTCTGATTGTCATATTA+AGG - chr5.2:1138522-1138541 MS.gene21845:CDS 30.0%
TTAATATGACAATCAGAACG+AGG + chr5.2:1138523-1138542 None:intergenic 30.0%
! AACCATATTATCAAAGGCAT+TGG - chr5.2:1138348-1138367 MS.gene21845:CDS 30.0%
! AGTTAAGGGTGTAAATTGAA+GGG + chr5.2:1138155-1138174 None:intergenic 30.0%
TGTGAGGTCTTCTTATAAAG+TGG - chr5.2:1138276-1138295 MS.gene21845:CDS 35.0%
TTCTGTCTGAAGAGAGTTAA+GGG + chr5.2:1138169-1138188 None:intergenic 35.0%
! GAGTTAAGGGTGTAAATTGA+AGG + chr5.2:1138156-1138175 None:intergenic 35.0%
! GTCCAATGCCTTTGATAATA+TGG + chr5.2:1138353-1138372 None:intergenic 35.0%
AAAGTGGTGATTGAGCATGA+GGG - chr5.2:1138292-1138311 MS.gene21845:CDS 40.0%
CTTCTGTCTGAAGAGAGTTA+AGG + chr5.2:1138170-1138189 None:intergenic 40.0%
TAAAGTGGTGATTGAGCATG+AGG - chr5.2:1138291-1138310 MS.gene21845:CDS 40.0%
TCACAGTGAAAGTGAGTGAT+GGG + chr5.2:1138261-1138280 None:intergenic 40.0%
!! TTATCAAAGGCATTGGACTC+TGG - chr5.2:1138355-1138374 MS.gene21845:CDS 40.0%
AAGCTTACAGTCATGAGGCA+CGG + chr5.2:1138386-1138405 None:intergenic 45.0%
ACAATCAGAACGAGGGTAAG+AGG + chr5.2:1138515-1138534 None:intergenic 45.0%
ACTCCAAGCTTACAGTCATG+AGG + chr5.2:1138391-1138410 None:intergenic 45.0%
CATCACTCACTTTCACTGTG+AGG - chr5.2:1138260-1138279 MS.gene21845:CDS 45.0%
CTCACAGTGAAAGTGAGTGA+TGG + chr5.2:1138262-1138281 None:intergenic 45.0%
GTGTTGAGCTGAGAAGGATA+GGG + chr5.2:1138211-1138230 None:intergenic 45.0%
TGTGTTGAGCTGAGAAGGAT+AGG + chr5.2:1138212-1138231 None:intergenic 45.0%
TGTTGAGCTGAGAAGGATAG+GGG + chr5.2:1138210-1138229 None:intergenic 45.0%
! AGTGATGATGTTGTAGAGCG+TGG - chr5.2:1138472-1138491 MS.gene21845:CDS 45.0%
AGGATAGGGGAGCTTAGTTG+TGG + chr5.2:1138197-1138216 None:intergenic 50.0%
ATGGGTGTGAACCTGAACCA+AGG + chr5.2:1138243-1138262 None:intergenic 50.0%
GATAGGGGAGCTTAGTTGTG+GGG + chr5.2:1138195-1138214 None:intergenic 50.0%
GGATAGGGGAGCTTAGTTGT+GGG + chr5.2:1138196-1138215 None:intergenic 50.0%
GGCACAGTTGATCAGAGTGA+TGG - chr5.2:1138442-1138461 MS.gene21845:CDS 50.0%
GTGCCTCATGACTGTAAGCT+TGG - chr5.2:1138385-1138404 MS.gene21845:CDS 50.0%
! GGTCTTGTGTTGAGCTGAGA+AGG + chr5.2:1138217-1138236 None:intergenic 50.0%
! TGTGCCACTGATAAGACGAG+CGG + chr5.2:1138428-1138447 None:intergenic 50.0%
ACAAGACCACGCCTTGGTTC+AGG - chr5.2:1138229-1138248 MS.gene21845:CDS 55.0%
CTCAACACAAGACCACGCCT+TGG - chr5.2:1138223-1138242 MS.gene21845:CDS 55.0%
TGTCCCGCTCGTCTTATCAG+TGG - chr5.2:1138421-1138440 MS.gene21845:CDS 55.0%
TGTGAACCTGAACCAAGGCG+TGG + chr5.2:1138238-1138257 None:intergenic 55.0%
! GTGCCACTGATAAGACGAGC+GGG + chr5.2:1138427-1138446 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 1138145 1138597 1138145 ID=MS.gene21845
chr5.2 mRNA 1138145 1138597 1138145 ID=MS.gene21845.t1;Parent=MS.gene21845
chr5.2 exon 1138145 1138597 1138145 ID=MS.gene21845.t1.exon1;Parent=MS.gene21845.t1
chr5.2 CDS 1138145 1138597 1138145 ID=cds.MS.gene21845.t1;Parent=MS.gene21845.t1
Gene Sequence

>MS.gene21845

ATGGCAACCCTTCAATTTACACCCTTAACTCTCTTCAGACAGAAGCACCCCACAACTAAGCTCCCCTATCCTTCTCAGCTCAACACAAGACCACGCCTTGGTTCAGGTTCACACCCATCACTCACTTTCACTGTGAGGTCTTCTTATAAAGTGGTGATTGAGCATGAGGGCAAGACTACACAGCTTGAAGTTGAACCTGATGAAACCATATTATCAAAGGCATTGGACTCTGGTTTGTCCGTGCCTCATGACTGTAAGCTTGGAGTTTGCATGACTTGTCCCGCTCGTCTTATCAGTGGCACAGTTGATCAGAGTGATGGTATGCTCAGTGATGATGTTGTAGAGCGTGGCTATGCACTCTTGTGTGCCTCTTACCCTCGTTCTGATTGTCATATTAAGGTTATAGCAGAGGATGAGCTACTCTCAATGCAGTTAGCTACAGCCAATGACTAA

Protein sequence

>MS.gene21845.t1

MATLQFTPLTLFRQKHPTTKLPYPSQLNTRPRLGSGSHPSLTFTVRSSYKVVIEHEGKTTQLEVEPDETILSKALDSGLSVPHDCKLGVCMTCPARLISGTVDQSDGMLSDDVVERGYALLCASYPRSDCHIKVIAEDELLSMQLATAND