AlfalfaGEDB | MS.gene000152

M. sativa cultivar XinJiangDaYe / MS.gene000152

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000152.t1	XP_003610722.1	94.8	154	4	2	1	151	1	153	1.40E-74	288.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000152.t1	O23344	81.1	106	20	0	46	151	49	154	1.4e-46	187.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000152.t1	G7K3M7	94.8	154	4	2	1	151	1	153	9.9e-75	288.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene74977	MS.gene000152	PPI
MS.gene67232	MS.gene000152	PPI
MS.gene88235	MS.gene000152	PPI
MS.gene030942	MS.gene000152	PPI
MS.gene47889	MS.gene000152	PPI
MS.gene27932	MS.gene000152	PPI
MS.gene067740	MS.gene000152	PPI
MS.gene84322	MS.gene000152	PPI
MS.gene065061	MS.gene000152	PPI
MS.gene030944	MS.gene000152	PPI
MS.gene35417	MS.gene000152	PPI
MS.gene034453	MS.gene000152	PPI
MS.gene000152	MS.gene029328	PPI
MS.gene000152	MS.gene43037	PPI
MS.gene000152	MS.gene66754	PPI
MS.gene000152	MS.gene03847	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000152.t1	MTR_5g006320	94.805	154	4	2	1	151	1	153	2.58e-103	292
MS.gene000152.t1	MTR_2g086510	34.416	154	83	4	7	142	2	155	6.62e-23	88.6
MS.gene000152.t1	MTR_4g053120	33.113	151	91	4	1	141	4	154	2.57e-22	87.0
MS.gene000152.t1	MTR_1g112050	37.302	126	72	4	22	141	29	153	3.72e-19	79.0
MS.gene000152.t1	MTR_1g112030	38.655	119	62	3	34	141	37	155	4.15e-19	79.0
MS.gene000152.t1	MTR_1g112030	38.655	119	62	3	34	141	41	159	4.86e-19	79.0
MS.gene000152.t1	MTR_2g006290	31.387	137	86	3	10	141	15	148	2.59e-18	76.6
MS.gene000152.t1	MTR_8g088720	34.400	125	77	2	20	143	28	148	7.77e-18	75.5
MS.gene000152.t1	MTR_4g098490	32.759	116	71	3	33	147	55	164	2.88e-14	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000152.t1	AT4G14890	81.132	106	20	0	46	151	49	154	5.44e-62	187
MS.gene000152.t1	AT2G27510	34.395	157	86	5	1	142	1	155	4.27e-23	89.4
MS.gene000152.t1	AT1G60950	34.532	139	81	6	10	142	14	148	2.73e-18	76.6
MS.gene000152.t1	AT5G10000	32.847	137	82	3	16	142	12	148	1.65e-16	72.0
MS.gene000152.t1	AT1G10960	30.435	138	88	4	10	142	14	148	4.84e-15	68.2
MS.gene000152.t1	AT1G32550	31.915	141	88	4	9	147	25	159	4.67e-14	66.2

Find 38 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTCCAATGCCTTTGATAATA+TGG	0.193692	5.4:+2039971	None:intergenic
TCGTTCTGATTGTCATATTA+AGG	0.194811	5.4:-2039799	MS.gene000152:CDS
TTCTGTCTGAAGAGAGTTAA+GGG	0.376635	5.4:+2040158	None:intergenic
TTTGATAATATGGTTTCATC+AGG	0.398760	5.4:+2039981	None:intergenic
CTGAAAGCTAAATTAGTCAT+TGG	0.415804	5.4:+2039734	None:intergenic
CTTCTGTCTGAAGAGAGTTA+AGG	0.435022	5.4:+2040157	None:intergenic
AGGATAGGGGAGCTTAGTTG+TGG	0.462415	5.4:+2040127	None:intergenic
GAGTTAAGGGTGTAAATTGA+AGG	0.462964	5.4:+2040171	None:intergenic
TTATCAAAGGCATTGGACTC+TGG	0.464183	5.4:-2039966	MS.gene000152:CDS
GGATAGGGGAGCTTAGTTGT+GGG	0.466042	5.4:+2040128	None:intergenic
AACCATATTATCAAAGGCAT+TGG	0.476989	5.4:-2039973	MS.gene000152:CDS
TGTGTTGAGCTGAGAAGGAT+AGG	0.480320	5.4:+2040112	None:intergenic
ACAAGACCACGCCTTGGTTC+AGG	0.487317	5.4:-2040092	MS.gene000152:CDS
TGTGAAGTCTTCTTATAAAG+TGG	0.499881	5.4:-2040045	MS.gene000152:CDS
ACAATCAGAACGAGGGTAAG+AGG	0.509900	5.4:+2039809	None:intergenic
GTGCCTCATGACTGTAAGCT+TGG	0.510320	5.4:-2039936	MS.gene000152:CDS
AGTTAAGGGTGTAAATTGAA+GGG	0.533055	5.4:+2040172	None:intergenic
GTGTTGAGCTGAGAAGGATA+GGG	0.540357	5.4:+2040113	None:intergenic
TAAAGTGGTGATTGAGCATG+AGG	0.567310	5.4:-2040030	MS.gene000152:CDS
TGATGAAACCATATTATCAA+AGG	0.569530	5.4:-2039979	MS.gene000152:CDS
GGTCTTGTGTTGAGCTGAGA+AGG	0.574174	5.4:+2040107	None:intergenic
CTCAACACAAGACCACGCCT+TGG	0.584774	5.4:-2040098	MS.gene000152:CDS
GTGCCACTGATAAGACGAGC+AGG	0.599960	5.4:+2039897	None:intergenic
TCATATTAAGGTTATAGCAG+AGG	0.608535	5.4:-2039787	MS.gene000152:CDS
TCACAGTGAAAGTGAGTGAT+GGG	0.611048	5.4:+2040063	None:intergenic
GGCACAGTTGATCAGAGTGA+TGG	0.619808	5.4:-2039879	MS.gene000152:CDS
TTCACAGTGAAAGTGAGTGA+TGG	0.627712	5.4:+2040062	None:intergenic
TGTTGAGCTGAGAAGGATAG+GGG	0.635174	5.4:+2040114	None:intergenic
TGTCCTGCTCGTCTTATCAG+TGG	0.649957	5.4:-2039900	MS.gene000152:CDS
AAGCTTACAGTCATGAGGCA+CGG	0.652590	5.4:+2039938	None:intergenic
GATAGGGGAGCTTAGTTGTG+GGG	0.665612	5.4:+2040129	None:intergenic
AGTGATGATGTTGTAGAGCG+TGG	0.677618	5.4:-2039849	MS.gene000152:CDS
ACTCCAAGCTTACAGTCATG+AGG	0.678735	5.4:+2039933	None:intergenic
TAATATGACAATCAGAACGA+GGG	0.699912	5.4:+2039802	None:intergenic
AAAGTGGTGATTGAGCATGA+GGG	0.724790	5.4:-2040029	MS.gene000152:CDS
TGTGAACCTGAACCAAGGCG+TGG	0.725548	5.4:+2040086	None:intergenic
TTAATATGACAATCAGAACG+AGG	0.733351	5.4:+2039801	None:intergenic
ATGGGTGTGAACCTGAACCA+AGG	0.761073	5.4:+2040081	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	TGATGAAACCATATTATCAA+AGG	-	chr5.4:2039946-2039965	MS.gene000152:CDS	25.0%
!!	TTTGATAATATGGTTTCATC+AGG	+	chr5.4:2039947-2039966	None:intergenic	25.0%
	TAATATGACAATCAGAACGA+GGG	+	chr5.4:2040126-2040145	None:intergenic	30.0%
	TCATATTAAGGTTATAGCAG+AGG	-	chr5.4:2040138-2040157	MS.gene000152:CDS	30.0%
	TCGTTCTGATTGTCATATTA+AGG	-	chr5.4:2040126-2040145	MS.gene000152:CDS	30.0%
	TGTGAAGTCTTCTTATAAAG+TGG	-	chr5.4:2039880-2039899	MS.gene000152:CDS	30.0%
	TTAATATGACAATCAGAACG+AGG	+	chr5.4:2040127-2040146	None:intergenic	30.0%
!	AACCATATTATCAAAGGCAT+TGG	-	chr5.4:2039952-2039971	MS.gene000152:CDS	30.0%
!	AGTTAAGGGTGTAAATTGAA+GGG	+	chr5.4:2039756-2039775	None:intergenic	30.0%
	TTCTGTCTGAAGAGAGTTAA+GGG	+	chr5.4:2039770-2039789	None:intergenic	35.0%
!	GAGTTAAGGGTGTAAATTGA+AGG	+	chr5.4:2039757-2039776	None:intergenic	35.0%
!	GTCCAATGCCTTTGATAATA+TGG	+	chr5.4:2039957-2039976	None:intergenic	35.0%
	AAAGTGGTGATTGAGCATGA+GGG	-	chr5.4:2039896-2039915	MS.gene000152:CDS	40.0%
	CTTCTGTCTGAAGAGAGTTA+AGG	+	chr5.4:2039771-2039790	None:intergenic	40.0%
	TAAAGTGGTGATTGAGCATG+AGG	-	chr5.4:2039895-2039914	MS.gene000152:CDS	40.0%
	TCACAGTGAAAGTGAGTGAT+GGG	+	chr5.4:2039865-2039884	None:intergenic	40.0%
	TTCACAGTGAAAGTGAGTGA+TGG	+	chr5.4:2039866-2039885	None:intergenic	40.0%
!!	TTATCAAAGGCATTGGACTC+TGG	-	chr5.4:2039959-2039978	MS.gene000152:CDS	40.0%
	AAGCTTACAGTCATGAGGCA+CGG	+	chr5.4:2039990-2040009	None:intergenic	45.0%
	ACAATCAGAACGAGGGTAAG+AGG	+	chr5.4:2040119-2040138	None:intergenic	45.0%
	ACTCCAAGCTTACAGTCATG+AGG	+	chr5.4:2039995-2040014	None:intergenic	45.0%
	GTGTTGAGCTGAGAAGGATA+GGG	+	chr5.4:2039815-2039834	None:intergenic	45.0%
	TGTGTTGAGCTGAGAAGGAT+AGG	+	chr5.4:2039816-2039835	None:intergenic	45.0%
	TGTTGAGCTGAGAAGGATAG+GGG	+	chr5.4:2039814-2039833	None:intergenic	45.0%
!	AGTGATGATGTTGTAGAGCG+TGG	-	chr5.4:2040076-2040095	MS.gene000152:CDS	45.0%
	AGGATAGGGGAGCTTAGTTG+TGG	+	chr5.4:2039801-2039820	None:intergenic	50.0%
	ATGGGTGTGAACCTGAACCA+AGG	+	chr5.4:2039847-2039866	None:intergenic	50.0%
	GATAGGGGAGCTTAGTTGTG+GGG	+	chr5.4:2039799-2039818	None:intergenic	50.0%
	GGATAGGGGAGCTTAGTTGT+GGG	+	chr5.4:2039800-2039819	None:intergenic	50.0%
	GGCACAGTTGATCAGAGTGA+TGG	-	chr5.4:2040046-2040065	MS.gene000152:CDS	50.0%
	GTGCCTCATGACTGTAAGCT+TGG	-	chr5.4:2039989-2040008	MS.gene000152:CDS	50.0%
	TGTCCTGCTCGTCTTATCAG+TGG	-	chr5.4:2040025-2040044	MS.gene000152:CDS	50.0%
!	GGTCTTGTGTTGAGCTGAGA+AGG	+	chr5.4:2039821-2039840	None:intergenic	50.0%
	ACAAGACCACGCCTTGGTTC+AGG	-	chr5.4:2039833-2039852	MS.gene000152:CDS	55.0%
	CTCAACACAAGACCACGCCT+TGG	-	chr5.4:2039827-2039846	MS.gene000152:CDS	55.0%
	TGTGAACCTGAACCAAGGCG+TGG	+	chr5.4:2039842-2039861	None:intergenic	55.0%
!	GTGCCACTGATAAGACGAGC+AGG	+	chr5.4:2040031-2040050	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	2039746	2040201	2039746	ID=MS.gene000152
chr5.4	mRNA	2039746	2040201	2039746	ID=MS.gene000152.t1;Parent=MS.gene000152
chr5.4	exon	2039746	2040201	2039746	ID=MS.gene000152.t1.exon1;Parent=MS.gene000152.t1
chr5.4	CDS	2039746	2040201	2039746	ID=cds.MS.gene000152.t1;Parent=MS.gene000152.t1

Gene Sequence

>MS.gene000152

ATGGCAACCCTTCAATTTACACCCTTAACTCTCTTCAGACAGAAGAAGCACCCCACAACTAAGCTCCCCTATCCTTCTCAGCTCAACACAAGACCACGCCTTGGTTCAGGTTCACACCCATCACTCACTTTCACTGTGAAGTCTTCTTATAAAGTGGTGATTGAGCATGAGGGCAAGACTACACAGCTTGAAGTTGAACCTGATGAAACCATATTATCAAAGGCATTGGACTCTGGTTTGTCCGTGCCTCATGACTGTAAGCTTGGAGTTTGCATGACTTGTCCTGCTCGTCTTATCAGTGGCACAGTTGATCAGAGTGATGGTATGCTCAGTGATGATGTTGTAGAGCGTGGCTATGCACTCTTGTGTGCCTCTTACCCTCGTTCTGATTGTCATATTAAGGTTATAGCAGAGGATGAGCTACTCTCAATGCAGTTAGCTACAGCCAATGACTAA

Protein sequence

>MS.gene000152.t1

MATLQFTPLTLFRQKKHPTTKLPYPSQLNTRPRLGSGSHPSLTFTVKSSYKVVIEHEGKTTQLEVEPDETILSKALDSGLSVPHDCKLGVCMTCPARLISGTVDQSDGMLSDDVVERGYALLCASYPRSDCHIKVIAEDELLSMQLATAND