AlfalfaGEDB | MS.gene66754

M. sativa cultivar XinJiangDaYe / MS.gene66754

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66754.t1	XP_003606105.2	97.4	155	4	0	1	155	1	155	4.70E-78	300.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66754.t1	P27788	62.1	153	54	1	3	155	4	152	1.8e-46	186.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66754.t1	G7JHA6	97.4	155	4	0	1	155	1	155	3.4e-78	300.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene030944	MS.gene66754	PPI
MS.gene030942	MS.gene66754	PPI
MS.gene024872	MS.gene66754	PPI
MS.gene47889	MS.gene66754	PPI
MS.gene67232	MS.gene66754	PPI
MS.gene000152	MS.gene66754	PPI
MS.gene27932	MS.gene66754	PPI
MS.gene92294	MS.gene66754	PPI
MS.gene21845	MS.gene66754	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66754.t1	MTR_4g053120	97.419	155	4	0	1	155	1	155	1.74e-110	310
MS.gene66754.t1	MTR_2g086510	73.548	155	41	0	1	155	1	155	2.13e-82	239
MS.gene66754.t1	MTR_1g112030	65.385	156	48	3	4	155	7	160	6.92e-66	198
MS.gene66754.t1	MTR_1g112030	65.385	156	48	3	4	155	3	156	8.17e-66	197
MS.gene66754.t1	MTR_1g112050	63.871	155	50	3	4	154	1	153	5.64e-63	190
MS.gene66754.t1	MTR_2g006290	60.000	130	38	3	28	154	30	148	2.64e-50	158
MS.gene66754.t1	MTR_8g088720	64.220	109	38	1	46	154	39	146	7.61e-47	149
MS.gene66754.t1	MTR_5g006320	41.414	99	56	2	56	154	47	143	3.02e-21	84.3
MS.gene66754.t1	MTR_4g098490	38.372	86	53	0	70	155	74	159	1.80e-17	75.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66754.t1	AT2G27510	59.355	155	60	2	4	155	1	155	2.26e-61	186
MS.gene66754.t1	AT1G60950	66.667	111	34	2	46	154	38	147	3.62e-48	152
MS.gene66754.t1	AT1G10960	57.778	135	48	5	19	151	17	144	1.95e-46	148
MS.gene66754.t1	AT5G10000	52.475	101	48	0	54	154	47	147	1.12e-37	126
MS.gene66754.t1	AT4G14890	46.316	95	49	2	60	154	52	144	2.79e-22	87.0
MS.gene66754.t1	AT1G32550	38.372	86	53	0	70	155	69	154	7.39e-18	76.3
MS.gene66754.t1	AT1G32550	35.294	102	47	3	70	155	69	167	4.79e-12	61.2

Find 41 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGAAGTTCCCTTCATCTTTC+GGG	0.151505	4.3:+46921673	MS.gene66754:CDS
TGTGCTGGAAAGATTGTTTC+TGG	0.226463	4.3:+46921903	MS.gene66754:CDS
GCTCCAGCACGGCATGAATA+AGG	0.234304	4.3:-46921870	None:intergenic
GCTTACAAGGTCAAGCTTAT+TGG	0.250602	4.3:+46921765	MS.gene66754:CDS
GCGAAGTTCCCTTCATCTTT+CGG	0.263582	4.3:+46921672	MS.gene66754:CDS
TGACTTCAAGCCACAAGATT+TGG	0.293119	4.3:-46921712	None:intergenic
TCAATCTGACGGCTCATTCT+TGG	0.346285	4.3:+46921935	MS.gene66754:CDS
CTTGGATGATAATCAACTTA+AGG	0.362806	4.3:+46921953	MS.gene66754:CDS
AACTGCAATGGCTGCTTACA+AGG	0.410013	4.3:+46921752	MS.gene66754:CDS
CTCCCTTATTCATGCCGTGC+TGG	0.418450	4.3:+46921867	MS.gene66754:CDS
TCTAAGATGTAAACATCATC+GGG	0.429231	4.3:-46921819	None:intergenic
CTCCAGCACGGCATGAATAA+GGG	0.444632	4.3:-46921869	None:intergenic
CAGTTTGAATCATGCATAGC+GGG	0.450595	4.3:-46921617	None:intergenic
GGAGCTTGCTCTACTTGTGC+TGG	0.452619	4.3:+46921888	MS.gene66754:CDS
TGGAAAGATTGTTTCTGGCT+CGG	0.490169	4.3:+46921908	MS.gene66754:CDS
TATGCAATCTGCGGTTGGAT+AGG	0.494449	4.3:-46922000	None:intergenic
GTCAAGCTTATTGGACCAGA+TGG	0.514160	4.3:+46921774	MS.gene66754:CDS
TTTGTAGACCCGAAAGATGA+AGG	0.518911	4.3:-46921681	None:intergenic
ATCTAAGATGTAAACATCAT+CGG	0.524266	4.3:-46921820	None:intergenic
AAGAGTGTTTCCAAATCTTG+TGG	0.543692	4.3:+46921702	MS.gene66754:CDS
GATGATAATCAACTTAAGGA+TGG	0.547500	4.3:+46921957	MS.gene66754:CDS
TCAATTATGCAATCTGCGGT+TGG	0.549164	4.3:-46922005	None:intergenic
TTAGATGCAGCAGAGGATGC+CGG	0.576824	4.3:+46921837	MS.gene66754:CDS
TTCATATAGAACAACTGCAA+TGG	0.601127	4.3:+46921740	MS.gene66754:CDS
GGCTCGGTGGATCAATCTGA+CGG	0.606093	4.3:+46921924	MS.gene66754:CDS
TTGTAGACCCGAAAGATGAA+GGG	0.611828	4.3:-46921680	None:intergenic
AAAGATTGTTTCTGGCTCGG+TGG	0.616952	4.3:+46921911	MS.gene66754:CDS
TAGCAATGGTTGATTGATTG+CGG	0.617701	4.3:-46921644	None:intergenic
ATAATTGAAACTCACAAGGA+AGG	0.627357	4.3:+46922020	MS.gene66754:CDS
GGAAACATTCACAGCTGACA+AGG	0.632858	4.3:-46921595	None:intergenic
TTACATCTTAGATGCAGCAG+AGG	0.636425	4.3:+46921830	MS.gene66754:CDS
AAGTAGAGCAAGCTCCAGCA+CGG	0.637088	4.3:-46921881	None:intergenic
GAACTTCGCTGTGATAGCAA+TGG	0.637553	4.3:-46921658	None:intergenic
TGAATAAGGGAGTTCAACGC+CGG	0.643639	4.3:-46921856	None:intergenic
TGCGGTTGGATAGGAAACAC+AGG	0.661542	4.3:-46921991	None:intergenic
AGTTTCAATTATGCAATCTG+CGG	0.680241	4.3:-46922009	None:intergenic
GCAGTTTGAATCATGCATAG+CGG	0.684816	4.3:-46921618	None:intergenic
TTGCATAATTGAAACTCACA+AGG	0.686967	4.3:+46922016	MS.gene66754:CDS
TAAGATGTAAACATCATCGG+GGG	0.710033	4.3:-46921817	None:intergenic
AGTTTGAATCATGCATAGCG+GGG	0.714486	4.3:-46921616	None:intergenic
CTAAGATGTAAACATCATCG+GGG	0.747899	4.3:-46921818	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCTAAGATGTAAACATCAT+CGG	-	chr4.3:46921823-46921842	None:intergenic	25.0%
	AGTTTCAATTATGCAATCTG+CGG	-	chr4.3:46922012-46922031	None:intergenic	30.0%
	ATAATTGAAACTCACAAGGA+AGG	+	chr4.3:46922020-46922039	MS.gene66754:CDS	30.0%
	CTTGGATGATAATCAACTTA+AGG	+	chr4.3:46921953-46921972	MS.gene66754:CDS	30.0%
	GATGATAATCAACTTAAGGA+TGG	+	chr4.3:46921957-46921976	MS.gene66754:CDS	30.0%
	TTCATATAGAACAACTGCAA+TGG	+	chr4.3:46921740-46921759	MS.gene66754:CDS	30.0%
	TTGCATAATTGAAACTCACA+AGG	+	chr4.3:46922016-46922035	MS.gene66754:CDS	30.0%
!	TCTAAGATGTAAACATCATC+GGG	-	chr4.3:46921822-46921841	None:intergenic	30.0%
	CTAAGATGTAAACATCATCG+GGG	-	chr4.3:46921821-46921840	None:intergenic	35.0%
	TAAGATGTAAACATCATCGG+GGG	-	chr4.3:46921820-46921839	None:intergenic	35.0%
	TAGCAATGGTTGATTGATTG+CGG	-	chr4.3:46921647-46921666	None:intergenic	35.0%
!	AACTCATTCTCTTTTCCATC+TGG	-	chr4.3:46921792-46921811	None:intergenic	35.0%
!!	AAGAGTGTTTCCAAATCTTG+TGG	+	chr4.3:46921702-46921721	MS.gene66754:CDS	35.0%
	AGTTTGAATCATGCATAGCG+GGG	-	chr4.3:46921619-46921638	None:intergenic	40.0%
	CAGTTTGAATCATGCATAGC+GGG	-	chr4.3:46921620-46921639	None:intergenic	40.0%
	GCAGTTTGAATCATGCATAG+CGG	-	chr4.3:46921621-46921640	None:intergenic	40.0%
	GCTTACAAGGTCAAGCTTAT+TGG	+	chr4.3:46921765-46921784	MS.gene66754:CDS	40.0%
	TCAATTATGCAATCTGCGGT+TGG	-	chr4.3:46922008-46922027	None:intergenic	40.0%
	TGACTTCAAGCCACAAGATT+TGG	-	chr4.3:46921715-46921734	None:intergenic	40.0%
	TGTGCTGGAAAGATTGTTTC+TGG	+	chr4.3:46921903-46921922	MS.gene66754:CDS	40.0%
	TTACATCTTAGATGCAGCAG+AGG	+	chr4.3:46921830-46921849	MS.gene66754:CDS	40.0%
	TTGTAGACCCGAAAGATGAA+GGG	-	chr4.3:46921683-46921702	None:intergenic	40.0%
	TTTGTAGACCCGAAAGATGA+AGG	-	chr4.3:46921684-46921703	None:intergenic	40.0%
!!	TGGAAAGATTGTTTCTGGCT+CGG	+	chr4.3:46921908-46921927	MS.gene66754:CDS	40.0%
	AACTGCAATGGCTGCTTACA+AGG	+	chr4.3:46921752-46921771	MS.gene66754:CDS	45.0%
	CGAAGTTCCCTTCATCTTTC+GGG	+	chr4.3:46921673-46921692	MS.gene66754:CDS	45.0%
	GAACTTCGCTGTGATAGCAA+TGG	-	chr4.3:46921661-46921680	None:intergenic	45.0%
	GCGAAGTTCCCTTCATCTTT+CGG	+	chr4.3:46921672-46921691	MS.gene66754:CDS	45.0%
	GGAAACATTCACAGCTGACA+AGG	-	chr4.3:46921598-46921617	None:intergenic	45.0%
	GTCAAGCTTATTGGACCAGA+TGG	+	chr4.3:46921774-46921793	MS.gene66754:CDS	45.0%
	TATGCAATCTGCGGTTGGAT+AGG	-	chr4.3:46922003-46922022	None:intergenic	45.0%
	TCAATCTGACGGCTCATTCT+TGG	+	chr4.3:46921935-46921954	MS.gene66754:CDS	45.0%
	TGAATAAGGGAGTTCAACGC+CGG	-	chr4.3:46921859-46921878	None:intergenic	45.0%
!!	AAAGATTGTTTCTGGCTCGG+TGG	+	chr4.3:46921911-46921930	MS.gene66754:CDS	45.0%
	AAGTAGAGCAAGCTCCAGCA+CGG	-	chr4.3:46921884-46921903	None:intergenic	50.0%
	CTCCAGCACGGCATGAATAA+GGG	-	chr4.3:46921872-46921891	None:intergenic	50.0%
	TGCGGTTGGATAGGAAACAC+AGG	-	chr4.3:46921994-46922013	None:intergenic	50.0%
	TTAGATGCAGCAGAGGATGC+CGG	+	chr4.3:46921837-46921856	MS.gene66754:CDS	50.0%
	CTCCCTTATTCATGCCGTGC+TGG	+	chr4.3:46921867-46921886	MS.gene66754:CDS	55.0%
	GCTCCAGCACGGCATGAATA+AGG	-	chr4.3:46921873-46921892	None:intergenic	55.0%
!	GGAGCTTGCTCTACTTGTGC+TGG	+	chr4.3:46921888-46921907	MS.gene66754:CDS	55.0%
!!	GGCTCGGTGGATCAATCTGA+CGG	+	chr4.3:46921924-46921943	MS.gene66754:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	46921588	46922055	46921588	ID=MS.gene66754
chr4.3	mRNA	46921588	46922055	46921588	ID=MS.gene66754.t1;Parent=MS.gene66754
chr4.3	exon	46921588	46922055	46921588	ID=MS.gene66754.t1.exon1;Parent=MS.gene66754.t1
chr4.3	CDS	46921588	46922055	46921588	ID=cds.MS.gene66754.t1;Parent=MS.gene66754.t1

Gene Sequence

>MS.gene66754

ATGGCATCCTTGTCAGCTGTGAATGTTTCCCCGCTATGCATGATTCAAACTGCAAACCGCAATCAATCAACCATTGCTATCACAGCGAAGTTCCCTTCATCTTTCGGGTCTACAAAGAGTGTTTCCAAATCTTGTGGCTTGAAGTCATCATCTTCATATAGAACAACTGCAATGGCTGCTTACAAGGTCAAGCTTATTGGACCAGATGGAAAAGAGAATGAGTTTGACGCCCCCGATGATGTTTACATCTTAGATGCAGCAGAGGATGCCGGCGTTGAACTCCCTTATTCATGCCGTGCTGGAGCTTGCTCTACTTGTGCTGGAAAGATTGTTTCTGGCTCGGTGGATCAATCTGACGGCTCATTCTTGGATGATAATCAACTTAAGGATGGCTTTGTGCTTACCTGTGTTTCCTATCCAACCGCAGATTGCATAATTGAAACTCACAAGGAAGGCGAACTCTACTGA

Protein sequence

>MS.gene66754.t1

MASLSAVNVSPLCMIQTANRNQSTIAITAKFPSSFGSTKSVSKSCGLKSSSSYRTTAMAAYKVKLIGPDGKENEFDAPDDVYILDAAEDAGVELPYSCRAGACSTCAGKIVSGSVDQSDGSFLDDNQLKDGFVLTCVSYPTADCIIETHKEGELY