AlfalfaGEDB | MS.gene030944

M. sativa cultivar XinJiangDaYe / MS.gene030944

KEGG: K02639
GO: GO:0003674 GO:0003824 GO:0005488 GO:0006091 GO:0008150 GO:0008152 GO:0009055 GO:0009987 GO:0016491 GO:0022900 GO:0044237 GO:0048037 GO:0051536 GO:0051537 GO:0051540 GO:0055114
PFAM: PF00111.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030944.t1	XP_003629958.1	97.3	148	4	0	1	148	1	148	2.70E-75	291.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030944.t1	Q43517	67.1	143	47	0	6	148	2	144	3.6e-52	205.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030944.t1	G7L8U4	97.3	148	4	0	1	148	1	148	2.0e-75	291.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene030944	MS.gene66754	PPI
MS.gene030944	MS.gene03847	PPI
MS.gene030944	MS.gene029328	PPI
MS.gene030944	MS.gene35417	PPI
MS.gene030944	MS.gene43037	PPI
MS.gene030944	MS.gene024872	PPI
MS.gene74977	MS.gene030944	PPI
MS.gene030944	MS.gene000152	PPI
MS.gene034453	MS.gene030944	PPI
MS.gene92294	MS.gene030944	PPI
MS.gene067740	MS.gene030944	PPI
MS.gene84322	MS.gene030944	PPI
MS.gene065061	MS.gene030944	PPI
MS.gene21845	MS.gene030944	PPI
MS.gene030944	MS.gene88235	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030944.t1	MTR_8g088720	97.297	148	4	0	1	148	1	148	7.18e-107	301
MS.gene030944.t1	MTR_2g006290	63.816	152	44	3	4	148	3	150	1.87e-63	191
MS.gene030944.t1	MTR_1g112030	56.164	146	57	5	4	146	18	159	1.57e-50	159
MS.gene030944.t1	MTR_1g112030	56.164	146	57	5	4	146	14	155	1.65e-50	159
MS.gene030944.t1	MTR_1g112050	55.782	147	62	3	1	146	9	153	6.20e-50	157
MS.gene030944.t1	MTR_2g086510	61.789	123	45	2	25	146	33	154	1.05e-49	156
MS.gene030944.t1	MTR_4g053120	57.037	135	51	3	19	146	20	154	5.77e-48	152
MS.gene030944.t1	MTR_5g006320	36.634	101	63	1	48	148	46	145	1.67e-17	74.7
MS.gene030944.t1	MTR_4g098490	36.585	82	52	0	65	146	77	158	3.29e-16	72.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene030944.t1	AT1G60950	64.000	150	49	3	1	147	1	148	1.03e-65	197
MS.gene030944.t1	AT1G10960	61.333	150	53	3	1	147	1	148	5.54e-59	180
MS.gene030944.t1	AT2G27510	50.658	152	65	4	5	146	3	154	1.99e-44	143
MS.gene030944.t1	AT5G10000	43.182	132	71	3	19	147	18	148	1.23e-32	113
MS.gene030944.t1	AT4G14890	35.333	150	80	4	3	148	10	146	7.54e-17	72.8
MS.gene030944.t1	AT1G32550	40.244	82	49	0	65	146	72	153	1.26e-16	73.2
MS.gene030944.t1	AT1G32550	34.783	92	44	2	71	146	75	166	5.22e-12	60.8

Find 56 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACGTGGGTCAGACTTTATT+TGG	0.300570	4.3:+11506968	MS.gene030944:CDS
CCACGTTAGGGAATGCTGTT+AGG	0.301806	4.3:-11506952	None:intergenic
GTAAGTTCTTTAGTTCCATC+TGG	0.311839	4.3:-11507055	None:intergenic
GCTGTTAGGGTTGTGTTCAT+TGG	0.358114	4.3:-11506938	None:intergenic
ACCACCACAGCAGCCCTATC+AGG	0.384793	4.3:+11506881	MS.gene030944:CDS
TGCTCACCATGGTTCCTGAT+AGG	0.397541	4.3:-11506895	None:intergenic
TGTGCTGGAAAAGTTGTAGA+AGG	0.411785	4.3:+11507166	MS.gene030944:CDS
AATAAAGTCTGACCCACGTT+AGG	0.420347	4.3:-11506965	None:intergenic
TCGAGAATGTACTCATTATC+CGG	0.426007	4.3:-11507082	None:intergenic
GATGAGCTTGATCTTGTAAC+TGG	0.426566	4.3:-11507029	None:intergenic
CTTCCTTACTCATGCAGGGC+TGG	0.437541	4.3:+11507130	MS.gene030944:CDS
GGTGCTTGCTCTTCATGTGC+TGG	0.439901	4.3:+11507151	MS.gene030944:CDS
TCGATCTTCCTTACTCATGC+AGG	0.452165	4.3:+11507125	MS.gene030944:CDS
TAAGTTCTTTAGTTCCATCT+GGG	0.455484	4.3:-11507054	None:intergenic
GCTCACCATGGTTCCTGATA+GGG	0.466904	4.3:-11506894	None:intergenic
TCCTGATAGGGCTGCTGTGG+TGG	0.470793	4.3:-11506882	None:intergenic
CATTGGCTGCCTCCTGATGA+AGG	0.472321	4.3:-11506921	None:intergenic
ACTTTATTTGGTGTGAAGGC+CGG	0.475879	4.3:+11506980	MS.gene030944:CDS
TGATAGGGCTGCTGTGGTGG+TGG	0.476979	4.3:-11506879	None:intergenic
TGGTGAGCACCTCCTTCATC+AGG	0.483290	4.3:+11506909	MS.gene030944:CDS
CACGTTAGGGAATGCTGTTA+GGG	0.501804	4.3:-11506951	None:intergenic
GCACCAGCCCTGCATGAGTA+AGG	0.502602	4.3:-11507133	None:intergenic
GATGATGATCAATTAAGTGC+TGG	0.507761	4.3:+11507220	MS.gene030944:CDS
TCAGACTTTATTTGGTGTGA+AGG	0.514081	4.3:+11506976	MS.gene030944:CDS
ATAAAGTCTGACCCACGTTA+GGG	0.523812	4.3:-11506964	None:intergenic
AAAAGTTGTAGAAGGGGAAG+TGG	0.534366	4.3:+11507174	MS.gene030944:CDS
ATCAAGCTCATCACCCCAGA+TGG	0.540257	4.3:+11507040	MS.gene030944:CDS
CTTGATCTTGTAACTGGCCA+TGG	0.544201	4.3:-11507023	None:intergenic
ATGATGATCAATTAAGTGCT+GGG	0.551795	4.3:+11507221	MS.gene030944:CDS
AAGATCGATGCCTTGTTCCT+CGG	0.553403	4.3:-11507110	None:intergenic
CTCGACGTAGCCGAGGAACA+AGG	0.555180	4.3:+11507100	MS.gene030944:CDS
CACTTAATTGATCATCATCA+AGG	0.556300	4.3:-11507216	None:intergenic
AACTAAAGAACTTACCTGCC+CGG	0.559896	4.3:+11507063	MS.gene030944:CDS
TTTGGTGTGAAGGCCGGACG+TGG	0.560810	4.3:+11506986	MS.gene030944:CDS
AATGTACTCATTATCCGGGC+AGG	0.566913	4.3:-11507077	None:intergenic
CGATCTTCCTTACTCATGCA+GGG	0.578511	4.3:+11507126	MS.gene030944:CDS
GTGCTGGAAAAGTTGTAGAA+GGG	0.579438	4.3:+11507167	MS.gene030944:CDS
GGTTCCTGATAGGGCTGCTG+TGG	0.579627	4.3:-11506885	None:intergenic
AGTGATACGACTTCCACGTC+CGG	0.584494	4.3:-11506999	None:intergenic
CGAGAATGTACTCATTATCC+GGG	0.584894	4.3:-11507081	None:intergenic
CCTTGTGTGTCTCAATAACA+AGG	0.591064	4.3:-11507279	None:intergenic
CCTAACAGCATTCCCTAACG+TGG	0.597665	4.3:+11506952	MS.gene030944:CDS
TGCTGGAAAAGTTGTAGAAG+GGG	0.598351	4.3:+11507168	MS.gene030944:CDS
TGGAAGTCGTATCACTGCCA+TGG	0.614707	4.3:+11507006	MS.gene030944:CDS
GTACATTCTCGACGTAGCCG+AGG	0.630338	4.3:+11507093	MS.gene030944:CDS
AAGTTCTTTAGTTCCATCTG+GGG	0.632400	4.3:-11507053	None:intergenic
AGCAGCCCTATCAGGAACCA+TGG	0.637647	4.3:+11506889	MS.gene030944:CDS
TGGCTGCCTCCTGATGAAGG+AGG	0.644050	4.3:-11506918	None:intergenic
GTGTGTCTCAATAACAAGGT+CGG	0.646026	4.3:-11507275	None:intergenic
CCTTGTTATTGAGACACACA+AGG	0.649100	4.3:+11507279	MS.gene030944:CDS
GATGAAGGAGGTGCTCACCA+TGG	0.650251	4.3:-11506906	None:intergenic
TCAATAACAAGGTCGGAACG+AGG	0.657316	4.3:-11507268	None:intergenic
CAATAACAAGGTCGGAACGA+GGG	0.670427	4.3:-11507267	None:intergenic
TGAGCACCTCCTTCATCAGG+AGG	0.676610	4.3:+11506912	MS.gene030944:CDS
CTAACAGCATTCCCTAACGT+GGG	0.718396	4.3:+11506953	MS.gene030944:CDS
TGTTATTGAGACACACAAGG+AGG	0.740119	4.3:+11507282	MS.gene030944:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGATGATCAATTAAGTGCT+GGG	+	chr4.3:11507221-11507240	MS.gene030944:CDS	30.0%
	CACTTAATTGATCATCATCA+AGG	-	chr4.3:11507219-11507238	None:intergenic	30.0%
!	TAAGTTCTTTAGTTCCATCT+GGG	-	chr4.3:11507057-11507076	None:intergenic	30.0%
	GATGATGATCAATTAAGTGC+TGG	+	chr4.3:11507220-11507239	MS.gene030944:CDS	35.0%
	TCGAGAATGTACTCATTATC+CGG	-	chr4.3:11507085-11507104	None:intergenic	35.0%
!	AAGTTCTTTAGTTCCATCTG+GGG	-	chr4.3:11507056-11507075	None:intergenic	35.0%
!	GTAAGTTCTTTAGTTCCATC+TGG	-	chr4.3:11507058-11507077	None:intergenic	35.0%
!!	TCAGACTTTATTTGGTGTGA+AGG	+	chr4.3:11506976-11506995	MS.gene030944:CDS	35.0%
	AAAAGTTGTAGAAGGGGAAG+TGG	+	chr4.3:11507174-11507193	MS.gene030944:CDS	40.0%
	AACGTGGGTCAGACTTTATT+TGG	+	chr4.3:11506968-11506987	MS.gene030944:CDS	40.0%
	AACTAAAGAACTTACCTGCC+CGG	+	chr4.3:11507063-11507082	MS.gene030944:CDS	40.0%
	AATAAAGTCTGACCCACGTT+AGG	-	chr4.3:11506968-11506987	None:intergenic	40.0%
	ATAAAGTCTGACCCACGTTA+GGG	-	chr4.3:11506967-11506986	None:intergenic	40.0%
	CCTTGTGTGTCTCAATAACA+AGG	-	chr4.3:11507282-11507301	None:intergenic	40.0%
	CGAGAATGTACTCATTATCC+GGG	-	chr4.3:11507084-11507103	None:intergenic	40.0%
	GATGAGCTTGATCTTGTAAC+TGG	-	chr4.3:11507032-11507051	None:intergenic	40.0%
	GTGCTGGAAAAGTTGTAGAA+GGG	+	chr4.3:11507167-11507186	MS.gene030944:CDS	40.0%
	GTGTGTCTCAATAACAAGGT+CGG	-	chr4.3:11507278-11507297	None:intergenic	40.0%
	TGCTGGAAAAGTTGTAGAAG+GGG	+	chr4.3:11507168-11507187	MS.gene030944:CDS	40.0%
	TGTGCTGGAAAAGTTGTAGA+AGG	+	chr4.3:11507166-11507185	MS.gene030944:CDS	40.0%
	TGTTATTGAGACACACAAGG+AGG	+	chr4.3:11507282-11507301	MS.gene030944:CDS	40.0%
!!	ACTTTATTTGGTGTGAAGGC+CGG	+	chr4.3:11506980-11506999	MS.gene030944:CDS	40.0%
!!	CCTTGTTATTGAGACACACA+AGG	+	chr4.3:11507279-11507298	MS.gene030944:CDS	40.0%
	AATGTACTCATTATCCGGGC+AGG	-	chr4.3:11507080-11507099	None:intergenic	45.0%
	CAATAACAAGGTCGGAACGA+GGG	-	chr4.3:11507270-11507289	None:intergenic	45.0%
	CACGTTAGGGAATGCTGTTA+GGG	-	chr4.3:11506954-11506973	None:intergenic	45.0%
	CGATCTTCCTTACTCATGCA+GGG	+	chr4.3:11507126-11507145	MS.gene030944:CDS	45.0%
	CTAACAGCATTCCCTAACGT+GGG	+	chr4.3:11506953-11506972	MS.gene030944:CDS	45.0%
	CTTGATCTTGTAACTGGCCA+TGG	-	chr4.3:11507026-11507045	None:intergenic	45.0%
	GCTGTTAGGGTTGTGTTCAT+TGG	-	chr4.3:11506941-11506960	None:intergenic	45.0%
	TCAATAACAAGGTCGGAACG+AGG	-	chr4.3:11507271-11507290	None:intergenic	45.0%
	TCGATCTTCCTTACTCATGC+AGG	+	chr4.3:11507125-11507144	MS.gene030944:CDS	45.0%
!	AAGATCGATGCCTTGTTCCT+CGG	-	chr4.3:11507113-11507132	None:intergenic	45.0%
	AGTGATACGACTTCCACGTC+CGG	-	chr4.3:11507002-11507021	None:intergenic	50.0%
	ATCAAGCTCATCACCCCAGA+TGG	+	chr4.3:11507040-11507059	MS.gene030944:CDS	50.0%
	CCACGTTAGGGAATGCTGTT+AGG	-	chr4.3:11506955-11506974	None:intergenic	50.0%
	CCTAACAGCATTCCCTAACG+TGG	+	chr4.3:11506952-11506971	MS.gene030944:CDS	50.0%
	GCTCACCATGGTTCCTGATA+GGG	-	chr4.3:11506897-11506916	None:intergenic	50.0%
	TGCTCACCATGGTTCCTGAT+AGG	-	chr4.3:11506898-11506917	None:intergenic	50.0%
	TGGAAGTCGTATCACTGCCA+TGG	+	chr4.3:11507006-11507025	MS.gene030944:CDS	50.0%
	AGCAGCCCTATCAGGAACCA+TGG	+	chr4.3:11506889-11506908	MS.gene030944:CDS	55.0%
	CTTCCTTACTCATGCAGGGC+TGG	+	chr4.3:11507130-11507149	MS.gene030944:CDS	55.0%
	GGTGCTTGCTCTTCATGTGC+TGG	+	chr4.3:11507151-11507170	MS.gene030944:CDS	55.0%
	GTACATTCTCGACGTAGCCG+AGG	+	chr4.3:11507093-11507112	MS.gene030944:CDS	55.0%
	TGAGCACCTCCTTCATCAGG+AGG	+	chr4.3:11506912-11506931	MS.gene030944:CDS	55.0%
	TGGTGAGCACCTCCTTCATC+AGG	+	chr4.3:11506909-11506928	MS.gene030944:CDS	55.0%
!	CATTGGCTGCCTCCTGATGA+AGG	-	chr4.3:11506924-11506943	None:intergenic	55.0%
!!	GATGAAGGAGGTGCTCACCA+TGG	-	chr4.3:11506909-11506928	None:intergenic	55.0%
	ACCACCACAGCAGCCCTATC+AGG	+	chr4.3:11506881-11506900	MS.gene030944:CDS	60.0%
	CTCGACGTAGCCGAGGAACA+AGG	+	chr4.3:11507100-11507119	MS.gene030944:CDS	60.0%
	GCACCAGCCCTGCATGAGTA+AGG	-	chr4.3:11507136-11507155	None:intergenic	60.0%
	GGTTCCTGATAGGGCTGCTG+TGG	-	chr4.3:11506888-11506907	None:intergenic	60.0%
	TCCTGATAGGGCTGCTGTGG+TGG	-	chr4.3:11506885-11506904	None:intergenic	60.0%
	TGATAGGGCTGCTGTGGTGG+TGG	-	chr4.3:11506882-11506901	None:intergenic	60.0%
	TGGCTGCCTCCTGATGAAGG+AGG	-	chr4.3:11506921-11506940	None:intergenic	60.0%
!	TTTGGTGTGAAGGCCGGACG+TGG	+	chr4.3:11506986-11507005	MS.gene030944:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	11506875	11507321	11506875	ID=MS.gene030944
chr4.3	mRNA	11506875	11507321	11506875	ID=MS.gene030944.t1;Parent=MS.gene030944
chr4.3	exon	11506875	11507321	11506875	ID=MS.gene030944.t1.exon1;Parent=MS.gene030944.t1
chr4.3	CDS	11506875	11507321	11506875	ID=cds.MS.gene030944.t1;Parent=MS.gene030944.t1

Gene Sequence

>MS.gene030944

ATGGCCACCACCACAGCAGCCCTATCAGGAACCATGGTGAGCACCTCCTTCATCAGGAGGCAGCCAATGAACACAACCCTAACAGCATTCCCTAACGTGGGTCAGACTTTATTTGGTGTGAAGGCCGGACGTGGAAGTCGTATCACTGCCATGGCCAGTTACAAGATCAAGCTCATCACCCCAGATGGAACTAAAGAACTTACCTGCCCGGATAATGAGTACATTCTCGACGTAGCCGAGGAACAAGGCATCGATCTTCCTTACTCATGCAGGGCTGGTGCTTGCTCTTCATGTGCTGGAAAAGTTGTAGAAGGGGAAGTGGATAATTCAGATAATAGCTTCCTTGATGATGATCAATTAAGTGCTGGGTTTGTTCTCACATGTGTTGCTTACCCTCGTTCCGACCTTGTTATTGAGACACACAAGGAGGAAGATCTTGTTTCTTAA

Protein sequence

>MS.gene030944.t1

MATTTAALSGTMVSTSFIRRQPMNTTLTAFPNVGQTLFGVKAGRGSRITAMASYKIKLITPDGTKELTCPDNEYILDVAEEQGIDLPYSCRAGACSSCAGKVVEGEVDNSDNSFLDDDQLSAGFVLTCVAYPRSDLVIETHKEEDLVS