Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032967.t1 | XP_024629315.1 | 82.6 | 470 | 74 | 4 | 1 | 470 | 1 | 462 | 3.00E-213 | 751.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032967.t1 | Q557M8 | 22.4 | 393 | 222 | 10 | 8 | 382 | 14 | 341 | 1.5e-11 | 72.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032967.t1 | A0A396IJ19 | 89.8 | 470 | 48 | 0 | 1 | 470 | 1 | 470 | 3.2e-212 | 747.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene032967 | MS.gene08242 | PPI |
| MS.gene032967 | MS.gene31944 | PPI |
| MS.gene032967 | MS.gene031871 | PPI |
| MS.gene032967 | MS.gene33782 | PPI |
| MS.gene032967 | MS.gene052488 | PPI |
| MS.gene032967 | MS.gene007951 | PPI |
| MS.gene032967 | MS.gene007950 | PPI |
| MS.gene032967 | MS.gene39443 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032967.t1 | MTR_8g077000 | 84.394 | 487 | 51 | 6 | 1 | 470 | 1 | 479 | 0.0 | 780 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene032967.t1 | AT1G03280 | 59.227 | 466 | 156 | 14 | 1 | 451 | 12 | 458 | 2.21e-178 | 508 |
| MS.gene032967.t1 | AT1G03280 | 59.227 | 466 | 156 | 14 | 1 | 451 | 12 | 458 | 2.21e-178 | 508 |
| MS.gene032967.t1 | AT1G03280 | 59.227 | 466 | 156 | 14 | 1 | 451 | 12 | 458 | 2.21e-178 | 508 |
| MS.gene032967.t1 | AT4G20340 | 52.174 | 460 | 192 | 12 | 1 | 451 | 13 | 453 | 1.85e-145 | 424 |
| MS.gene032967.t1 | AT4G20340 | 52.174 | 460 | 192 | 12 | 1 | 451 | 13 | 453 | 1.85e-145 | 424 |
| MS.gene032967.t1 | AT4G20810 | 47.548 | 469 | 185 | 16 | 8 | 469 | 1 | 415 | 1.79e-130 | 384 |
| MS.gene032967.t1 | AT4G20340 | 52.542 | 413 | 169 | 12 | 48 | 451 | 4 | 398 | 8.29e-128 | 377 |
Find 100 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATCACTTCTTCCAGTTT+TGG | 0.162141 | 8.4:-16363303 | None:intergenic |
| AACTGACGGTACATCTTTAA+AGG | 0.182546 | 8.4:+16368844 | MS.gene032967:CDS |
| GACTTGCCTGTTCCAGAATT+TGG | 0.189652 | 8.4:+16366607 | MS.gene032967:CDS |
| ATCTGCTGCGTTCGCATCTT+GGG | 0.226916 | 8.4:-16369238 | None:intergenic |
| CATCTGCTGCGTTCGCATCT+TGG | 0.249410 | 8.4:-16369239 | None:intergenic |
| TCGTCCTCGTCGTCTTCTTC+TGG | 0.291126 | 8.4:-16371115 | None:intergenic |
| GGGCTGATTATCTCCTTTGC+TGG | 0.323516 | 8.4:-16363282 | None:intergenic |
| GCTTGAAGACTACCAAATTC+TGG | 0.326095 | 8.4:-16366619 | None:intergenic |
| AAATCTCATCTGGGATATAA+TGG | 0.332016 | 8.4:+16366703 | MS.gene032967:CDS |
| GAGAAATTGGTACTTGTTGT+AGG | 0.340168 | 8.4:-16363128 | None:intergenic |
| GGAGATAATCAGCCCAAAAC+TGG | 0.349455 | 8.4:+16363290 | MS.gene032967:CDS |
| ACGACGGCGTGAAAAGTTAA+AGG | 0.370019 | 8.4:+16365119 | MS.gene032967:CDS |
| TGGTGATTCTAAATCTCATC+TGG | 0.375711 | 8.4:+16366693 | MS.gene032967:CDS |
| TGGGAAGAGGCTCCTGTTAG+AGG | 0.378747 | 8.4:+16369336 | MS.gene032967:CDS |
| GAAGCCCGAGCTAGTGCAGC+TGG | 0.384987 | 8.4:+16366646 | MS.gene032967:CDS |
| AGAGAACAAGAACACCATTC+AGG | 0.401351 | 8.4:+16364798 | MS.gene032967:CDS |
| CACGCCCAGCTGCACTAGCT+CGG | 0.403153 | 8.4:-16366651 | None:intergenic |
| GCTTAAGAAGCAACGCGAAT+TGG | 0.412214 | 8.4:+16369197 | MS.gene032967:CDS |
| TTATTTCAGATACAATGCTT+TGG | 0.415674 | 8.4:+16364960 | MS.gene032967:intron |
| GGCTGAAGTGTACCTCTAAC+AGG | 0.418995 | 8.4:-16369348 | None:intergenic |
| GCAGCTGGGCGTGCAGCTAA+TGG | 0.419366 | 8.4:+16366661 | MS.gene032967:CDS |
| GTTGCTTTGTATGCAATTTG+AGG | 0.441479 | 8.4:-16363496 | None:intergenic |
| ATTTGGTAGTCTTCAAGCTT+GGG | 0.444477 | 8.4:+16366624 | MS.gene032967:CDS |
| ACGCCCAGCTGCACTAGCTC+GGG | 0.452951 | 8.4:-16366650 | None:intergenic |
| CGGTTGGAAGTACTGCTAGA+AGG | 0.463810 | 8.4:-16369264 | None:intergenic |
| AGGAAAAGAGGGAGAAGAAA+AGG | 0.464405 | 8.4:+16364494 | MS.gene032967:CDS |
| ATAAGTTAGCTGCTCAAGAC+GGG | 0.467809 | 8.4:+16365061 | MS.gene032967:CDS |
| AGATTATCACTAGAGATCAT+AGG | 0.477747 | 8.4:+16363552 | MS.gene032967:CDS |
| AATTTGGTAGTCTTCAAGCT+TGG | 0.481156 | 8.4:+16366623 | MS.gene032967:CDS |
| TTCCACTGTGAACGTTGTAA+TGG | 0.482559 | 8.4:+16365018 | MS.gene032967:CDS |
| AGACTACCAAATTCTGGAAC+AGG | 0.482675 | 8.4:-16366613 | None:intergenic |
| AAAGGACATGCTTCAGAAGA+TGG | 0.491664 | 8.4:+16365137 | MS.gene032967:CDS |
| CCTCCATGGATGATTACATC+AGG | 0.493168 | 8.4:+16368872 | MS.gene032967:CDS |
| AAATGCAATTGGAGCTTACA+AGG | 0.494474 | 8.4:+16371063 | MS.gene032967:CDS |
| TGTACATATATATTCCTGAA+TGG | 0.494787 | 8.4:-16364812 | None:intergenic |
| ACAGCGTGGAGAGGTGAAGC+AGG | 0.499690 | 8.4:+16368913 | MS.gene032967:CDS |
| TATGTACAAACTGTGGCAAA+AGG | 0.503511 | 8.4:+16364827 | MS.gene032967:CDS |
| TGAAGCTACGACAGGAAAAG+AGG | 0.510943 | 8.4:+16364482 | MS.gene032967:CDS |
| AGAAATTGGTACTTGTTGTA+GGG | 0.511459 | 8.4:-16363127 | None:intergenic |
| ACCTTGGTATCTCCGCTGTA+TGG | 0.515063 | 8.4:-16366736 | None:intergenic |
| TTGTTCTTGATGCGCTCACC+AGG | 0.522461 | 8.4:+16363342 | MS.gene032967:CDS |
| GTCTTCTTCTGGCTGAGCAT+CGG | 0.526811 | 8.4:-16371104 | None:intergenic |
| GACGATATCGACTGGGAGGA+AGG | 0.528222 | 8.4:+16371133 | MS.gene032967:CDS |
| TCTGCTGCGTTCGCATCTTG+GGG | 0.534141 | 8.4:-16369237 | None:intergenic |
| CGACTGACTTGATCTACTAA+AGG | 0.542394 | 8.4:-16366577 | None:intergenic |
| TGATGGTACCGAGTGGGAAG+AGG | 0.544924 | 8.4:+16369323 | MS.gene032967:CDS |
| ACGACGAGGACGATATCGAC+TGG | 0.550692 | 8.4:+16371125 | MS.gene032967:CDS |
| ATACCTGATGTAATCATCCA+TGG | 0.551552 | 8.4:-16368875 | None:intergenic |
| TTGATTTCAAGCCAACTTGG+CGG | 0.551942 | 8.4:-16369284 | None:intergenic |
| TTTCAAGCCAACTTGGCGGT+TGG | 0.553993 | 8.4:-16369280 | None:intergenic |
| TTTAGTAGATCAAGTCAGTC+GGG | 0.559290 | 8.4:+16366579 | MS.gene032967:CDS |
| CAGGTGGTAGTTGACTTCAA+TGG | 0.563361 | 8.4:+16368788 | MS.gene032967:intron |
| GATAAGTTAGCTGCTCAAGA+CGG | 0.564672 | 8.4:+16365060 | MS.gene032967:CDS |
| TTTATAGGATGAGTATGTCA+AGG | 0.564885 | 8.4:+16369158 | MS.gene032967:intron |
| TATGACGATCTTACCAGCAA+AGG | 0.565230 | 8.4:+16363269 | MS.gene032967:CDS |
| AGTACTTCCAACCGCCAAGT+TGG | 0.569471 | 8.4:+16369273 | MS.gene032967:CDS |
| TGCAGATATATGATGTGGTT+AGG | 0.570661 | 8.4:+16364734 | MS.gene032967:intron |
| AACAGGAGCCTCTTCCCACT+CGG | 0.576584 | 8.4:-16369331 | None:intergenic |
| GTATCTCCGCTGTATGGCAA+TGG | 0.577878 | 8.4:-16366730 | None:intergenic |
| AAGCCCGAGCTAGTGCAGCT+GGG | 0.582139 | 8.4:+16366647 | MS.gene032967:CDS |
| AAACGTGAGGAAGATGATGA+TGG | 0.590028 | 8.4:+16369306 | MS.gene032967:CDS |
| CTTTAGTAGATCAAGTCAGT+CGG | 0.591765 | 8.4:+16366578 | MS.gene032967:CDS |
| GTTGACTTCAATGGTACTGA+AGG | 0.599548 | 8.4:+16368797 | MS.gene032967:CDS |
| GGTACTGAAGGCAAAGGAGA+GGG | 0.602003 | 8.4:+16368809 | MS.gene032967:CDS |
| GTCTGTGAATTGCACTGATG+TGG | 0.605194 | 8.4:-16368963 | None:intergenic |
| GGTGATTCTAAATCTCATCT+GGG | 0.606736 | 8.4:+16366694 | MS.gene032967:CDS |
| GAAGCTACGACAGGAAAAGA+GGG | 0.609436 | 8.4:+16364483 | MS.gene032967:CDS |
| ATGGGTTAGAGAAGAAGACT+TGG | 0.611007 | 8.4:+16363466 | MS.gene032967:CDS |
| GCAGCTAATGGAGATAATGC+TGG | 0.617199 | 8.4:+16366673 | MS.gene032967:CDS |
| GCCATACAGCGGAGATACCA+AGG | 0.618161 | 8.4:+16366735 | MS.gene032967:CDS |
| CGACGAGGACGATATCGACT+GGG | 0.621959 | 8.4:+16371126 | MS.gene032967:CDS |
| CACTAGAGATCATAGGAGAG+AGG | 0.622575 | 8.4:+16363559 | MS.gene032967:CDS |
| CTGCTGTCTAGATTATGCAC+AGG | 0.622608 | 8.4:+16364539 | MS.gene032967:CDS |
| GCTACAGCTGAAGCTACGAC+AGG | 0.624340 | 8.4:+16364474 | MS.gene032967:CDS |
| AGATGATGATGGTACCGAGT+GGG | 0.630560 | 8.4:+16369317 | MS.gene032967:CDS |
| TTCAATGGTACTGAAGGCAA+AGG | 0.631073 | 8.4:+16368803 | MS.gene032967:CDS |
| TAAGTTAGCTGCTCAAGACG+GGG | 0.634311 | 8.4:+16365062 | MS.gene032967:CDS |
| GAGCGCATCAAGAACAACTA+CGG | 0.638602 | 8.4:-16363336 | None:intergenic |
| TGGTACTGAAGGCAAAGGAG+AGG | 0.643856 | 8.4:+16368808 | MS.gene032967:CDS |
| ATGAGAATGCACGAAGACGA+CGG | 0.644965 | 8.4:+16365103 | MS.gene032967:CDS |
| TACGAAAGAACAGCGTGGAG+AGG | 0.645484 | 8.4:+16368904 | MS.gene032967:CDS |
| TTTGTTGCAGATATATGATG+TGG | 0.649334 | 8.4:+16364729 | MS.gene032967:intron |
| AGCAACTTCGTCGAACTCTG+CGG | 0.650459 | 8.4:+16363513 | MS.gene032967:CDS |
| GAATATATATGTACAAACTG+TGG | 0.654824 | 8.4:+16364820 | MS.gene032967:CDS |
| TGGCTTGAAATCAAAACGTG+AGG | 0.659414 | 8.4:+16369293 | MS.gene032967:CDS |
| TAGAGAAGAAGACTTGGCAA+AGG | 0.661782 | 8.4:+16363472 | MS.gene032967:CDS |
| GGTGTTAAATCTGAAACTGA+CGG | 0.664295 | 8.4:+16368830 | MS.gene032967:CDS |
| TGCAATTGGAGCTTACAAGG+TGG | 0.682571 | 8.4:+16371066 | MS.gene032967:CDS |
| AAGTTAGCTGCTCAAGACGG+GGG | 0.688054 | 8.4:+16365063 | MS.gene032967:CDS |
| GGACATGCTTCAGAAGATGG+AGG | 0.689485 | 8.4:+16365140 | MS.gene032967:CDS |
| ACGTTGTAATGGAAAACTAG+AGG | 0.692315 | 8.4:+16365029 | MS.gene032967:CDS |
| GGAGCACCATTGCCATACAG+CGG | 0.718354 | 8.4:+16366724 | MS.gene032967:CDS |
| AAGATGATGATGGTACCGAG+TGG | 0.721118 | 8.4:+16369316 | MS.gene032967:CDS |
| ACTGGAAGAAGTGATAACAG+AGG | 0.728511 | 8.4:+16363308 | MS.gene032967:CDS |
| TTCCATTACAACGTTCACAG+TGG | 0.729270 | 8.4:-16365020 | None:intergenic |
| CGAGGACGATATCGACTGGG+AGG | 0.742122 | 8.4:+16371129 | MS.gene032967:CDS |
| CCTGATGTAATCATCCATGG+AGG | 0.742710 | 8.4:-16368872 | None:intergenic |
| AATCTTACGAAAGAACAGCG+TGG | 0.751641 | 8.4:+16368899 | MS.gene032967:CDS |
| TCAGCCAGAAGAAGACGACG+AGG | 0.751885 | 8.4:+16371111 | MS.gene032967:CDS |
| ACAATGCTTTGGATGCACTG+CGG | 0.757429 | 8.4:+16364971 | MS.gene032967:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 16363118 | 16371159 | 16363118 | ID=MS.gene032967 |
| chr8.4 | mRNA | 16363118 | 16371159 | 16363118 | ID=MS.gene032967.t1;Parent=MS.gene032967 |
| chr8.4 | exon | 16363118 | 16363137 | 16363118 | ID=MS.gene032967.t1.exon1;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16363118 | 16363137 | 16363118 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| chr8.4 | exon | 16363241 | 16363363 | 16363241 | ID=MS.gene032967.t1.exon2;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16363241 | 16363363 | 16363241 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| chr8.4 | exon | 16363460 | 16363580 | 16363460 | ID=MS.gene032967.t1.exon3;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16363460 | 16363580 | 16363460 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| chr8.4 | exon | 16364435 | 16364560 | 16364435 | ID=MS.gene032967.t1.exon4;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16364435 | 16364560 | 16364435 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| chr8.4 | exon | 16364739 | 16364848 | 16364739 | ID=MS.gene032967.t1.exon5;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16364739 | 16364848 | 16364739 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| chr8.4 | exon | 16364969 | 16365161 | 16364969 | ID=MS.gene032967.t1.exon6;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16364969 | 16365161 | 16364969 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| chr8.4 | exon | 16366565 | 16366756 | 16366565 | ID=MS.gene032967.t1.exon7;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16366565 | 16366756 | 16366565 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| chr8.4 | exon | 16368791 | 16369027 | 16368791 | ID=MS.gene032967.t1.exon8;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16368791 | 16369027 | 16368791 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| chr8.4 | exon | 16369165 | 16369357 | 16369165 | ID=MS.gene032967.t1.exon9;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16369165 | 16369357 | 16369165 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| chr8.4 | exon | 16371062 | 16371159 | 16371062 | ID=MS.gene032967.t1.exon10;Parent=MS.gene032967.t1 |
| chr8.4 | CDS | 16371062 | 16371159 | 16371062 | ID=cds.MS.gene032967.t1;Parent=MS.gene032967.t1 |
| Gene Sequence |
| Protein sequence |
