AlfalfaGEDB | MS.gene034836

M. sativa cultivar XinJiangDaYe / MS.gene034836

KEGG: -
GO: -
PFAM: PF04535.15

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034836.t1	XP_013467943.1	95.9	196	8	0	1	196	1	196	1.10E-92	349.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034836.t1	P0DI40	78.1	196	39	1	1	196	1	192	1.5e-77	290.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034836.t1	A0A072VV58	95.9	196	8	0	1	196	1	196	8.0e-93	349.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene008354	MS.gene034836	PPI
MS.gene034836	MS.gene049677	PPI
MS.gene034836	MS.gene81804	PPI
MS.gene034836	MS.gene05033	PPI
MS.gene034836	MS.gene26200	PPI
MS.gene034836	MS.gene017305	PPI
MS.gene05032	MS.gene034836	PPI
MS.gene061583	MS.gene034836	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034836.t1	MTR_1g058540	95.918	196	8	0	1	196	1	196	2.61e-120	338
MS.gene034836.t1	MTR_1g058570	77.041	196	43	1	1	196	1	194	3.19e-93	270
MS.gene034836.t1	MTR_1g058560	80.102	196	38	1	1	196	1	195	1.15e-91	266
MS.gene034836.t1	MTR_2g086740	52.857	210	83	1	2	195	10	219	2.30e-59	185
MS.gene034836.t1	MTR_7g011090	45.263	190	100	1	7	196	1	186	3.33e-52	166
MS.gene034836.t1	MTR_4g081880	42.197	173	100	0	23	195	14	186	2.16e-47	154
MS.gene034836.t1	MTR_5g041900	40.722	194	102	2	4	196	5	186	1.46e-41	139
MS.gene034836.t1	MTR_2g086740	49.645	141	55	1	2	126	10	150	6.56e-34	118
MS.gene034836.t1	MTR_0004s0210	34.615	156	87	4	37	189	7	150	4.06e-18	77.8
MS.gene034836.t1	MTR_0097s0070	34.167	120	73	3	73	189	69	185	1.64e-12	63.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene034836.t1	AT2G36100	54.950	202	84	2	2	196	5	206	3.24e-68	207
MS.gene034836.t1	AT3G11550	60.500	200	74	1	2	196	5	204	2.73e-64	197
MS.gene034836.t1	AT2G27370	55.978	184	74	1	12	195	43	219	6.75e-64	197
MS.gene034836.t1	AT5G06200	58.586	198	77	2	2	195	5	201	1.03e-63	196
MS.gene034836.t1	AT5G15290	44.000	150	84	0	27	176	17	166	4.56e-44	145
MS.gene034836.t1	AT1G14160	40.000	165	99	0	32	196	45	209	8.32e-42	140
MS.gene034836.t1	AT1G03700	30.769	169	99	3	21	189	1	151	1.95e-17	76.3
MS.gene034836.t1	AT1G17200	26.984	189	125	5	9	193	2	181	2.36e-14	68.9
MS.gene034836.t1	AT4G03540	32.258	124	75	2	55	178	26	140	1.06e-13	66.2
MS.gene034836.t1	AT3G06390	27.820	133	91	2	38	165	37	169	5.71e-11	59.7

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Find 42 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGTAGGGATAAGACC+AGG	0.708096	1.4:+48578856	None:intergenic
AATCTGATCACTAGTTGCCA+TGG	0.596028	1.4:+48579189	None:intergenic
AATGTCAACCACTATTGATA+TGG	0.415102	1.4:-48579356	MS.gene034836:CDS
AGCAGCTGCCATGGTTTACT+TGG	0.359773	1.4:-48578234	MS.gene034836:CDS
AGCCTTGGTCCAGGTGCATG+AGG	0.573726	1.4:+48578813	None:intergenic
AGGGCGAATGATGGTTACTA+TGG	0.422728	1.4:+48578833	None:intergenic
AGGTGCATGAGGGCGAATGA+TGG	0.573876	1.4:+48578824	None:intergenic
ATCATTCGCCCTCATGCACC+TGG	0.484461	1.4:-48578822	MS.gene034836:CDS
ATCTCCAAATTGGTTGCAAA+TGG	0.439108	1.4:+48578178	None:intergenic
ATGACATTAGCCACTGCTAG+TGG	0.602800	1.4:-48578263	MS.gene034836:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATGATGGTTACTATGGAAAA+TGG	+	chr1.4:48578594-48578613	None:intergenic	30.0%
ATTACTCCTTGTGTTTCTTT+CGG	-	chr1.4:48578787-48578806	MS.gene034836:intron	30.0%
GAAGATAAATTGCTACAATG+AGG	-	chr1.4:48578928-48578947	MS.gene034836:intron	30.0%
TAATGTCATAAACACCTACA+CGG	+	chr1.4:48579157-48579176	None:intergenic	30.0%
TGGACTAAATAAATACACCT+AGG	+	chr1.4:48578391-48578410	None:intergenic	30.0%
ATCTCCAAATTGGTTGCAAA+TGG	+	chr1.4:48579256-48579275	None:intergenic	35.0%
GAGCACAAAAATCTCCAAAT+TGG	+	chr1.4:48579266-48579285	None:intergenic	35.0%
GAGGTTTAAATGTGACATAC+TGG	+	chr1.4:48578327-48578346	None:intergenic	35.0%
GGTTACTATGGAAAATGGTA+GGG	+	chr1.4:48578589-48578608	None:intergenic	35.0%
GTTATTGCAATGGCATTTGT+GGG	-	chr1.4:48578546-48578565	MS.gene034836:intron	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	48578076	48579377	48578076	ID=MS.gene034836
chr1.4	mRNA	48578076	48579377	48578076	ID=MS.gene034836.t1;Parent=MS.gene034836
chr1.4	exon	48579127	48579377	48579127	ID=MS.gene034836.t1.exon1;Parent=MS.gene034836.t1
chr1.4	CDS	48579127	48579377	48579127	ID=cds.MS.gene034836.t1;Parent=MS.gene034836.t1
chr1.4	exon	48578791	48578914	48578791	ID=MS.gene034836.t1.exon2;Parent=MS.gene034836.t1
chr1.4	CDS	48578791	48578914	48578791	ID=cds.MS.gene034836.t1;Parent=MS.gene034836.t1
chr1.4	exon	48578076	48578291	48578076	ID=MS.gene034836.t1.exon3;Parent=MS.gene034836.t1
chr1.4	CDS	48578076	48578291	48578076	ID=cds.MS.gene034836.t1;Parent=MS.gene034836.t1

Gene Sequence

>MS.gene034836

ATGTCAACCACTATTGATATGGCAGAATCAAGCAACGCTCATGTTAATGAAAAAGCTGCTTTAATTGGAGCCACTGCAAGGCCTGGAGGATGGAAAAAAGGCATAGCCATAATGGATTTTGTTCTAAGATTAGGTGCCATAGCAGCTTCTCTTGCTGCAACTATCACCATGGCAACTAGTGATCAGATTCTTCCTTTCTTCACTCAGTTCCTTCAATTTGAAGCTAGTTATGATAGCTTTTCAACTTTCCAGTATGTCACATTTAAACCTCTAACTTAAACAAATAATTTATATACTCCCTCCATTGTAAAACCTAGGTGTATTTATTTAGTCCAACACAAAATTTGAACTACACACATATTTATATACTTTTTATTTATATTTTGTTATTAATTTTGTGCTATATTCATAGAAATTAAATTAATTATGTCAAAAAATATTAATGTTTATTGTAAATATGCAGGTTTTTTGTTATTGCAATGGCATTTGTGGGTGGCTACCTGGTCTTATCCCTACCATTTTCCATAGTAACCATCATTCGCCCTCATGCACCTGGACCAAGGCTTTTCCTCATCATCGCAGACACAGTAATTATGCTCCTAATTTCCTAATTAATTCTTAATTTGAGTAAAATAATTTTACTTGAGAACCCTTCAAATTTTTTTTACATGAACATATATATTCTAGTAGGAACACATTTTAAATATAATTATTACTCCTTGTGTTTCTTTCGGCCTCTATTTATTTTCAAATGACCATTGCTTTATTGATTTTTTTTTAATATTCACATGTTATTTTCAAGATTAAAATTACATTTTTTTGCAATGTATAGTCATAATTAATTTATTTCAAGAAGATAAATTGCTACAATGAGGAAATGAACTATTAAGGATATAACAATAAAAATACTAACAATTTTATATAAAAAATAAAATAATACATTGATTTATTTAGTTAAGTACTTTTAAAAAACTGGAAAAAGAGAAACTATTATTTTAAAAGTGGTACTAGTAAATGTTTTATAATTATTTTTGTAAAGATTTGAAATTTTAGATTAAAGTTACAAACTCAAACTTTTCCGTGTAGGTGTTTATGACATTAGCCACTGCTAGTGGTGCTTCAGCAGCTGCCATGGTTTACTTGGCACACAATGGCAATCAAGACTCAAACTGGCTTGCCATTTGCAACCAATTTGGAGATTTTTGTGCTCAAACAAGTGGAGCAGTTGTGTCATGTTTTATTGCTGTGGTTGTTTTAATGGTGTTGGTTGTGATGTCTTCTTTGGCTCTTGGGAAGCATTGA

Protein sequence

>MS.gene034836.t1

MSTTIDMAESSNAHVNEKAALIGATARPGGWKKGIAIMDFVLRLGAIAASLAATITMATSDQILPFFTQFLQFEASYDSFSTFQFFVIAMAFVGGYLVLSLPFSIVTIIRPHAPGPRLFLIIADTVFMTLATASGASAAAMVYLAHNGNQDSNWLAICNQFGDFCAQTSGAVVSCFIAVVVLMVLVVMSSLALGKH