Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034838.t1 | XP_013467945.1 | 97.4 | 195 | 5 | 0 | 1 | 195 | 1 | 195 | 1.20E-91 | 345.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034838.t1 | P0DI39 | 84.0 | 194 | 30 | 1 | 1 | 194 | 1 | 193 | 1.9e-80 | 300.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034838.t1 | A0A072VJL1 | 97.4 | 195 | 5 | 0 | 1 | 195 | 1 | 195 | 8.8e-92 | 345.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene008354 | MS.gene034838 | PPI |
| MS.gene034838 | MS.gene81804 | PPI |
| MS.gene034838 | MS.gene049677 | PPI |
| MS.gene034838 | MS.gene05033 | PPI |
| MS.gene034838 | MS.gene061583 | PPI |
| MS.gene034838 | MS.gene26200 | PPI |
| MS.gene034838 | MS.gene017305 | PPI |
| MS.gene034838 | MS.gene05032 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034838.t1 | MTR_1g058560 | 98.462 | 195 | 3 | 0 | 1 | 195 | 1 | 195 | 6.17e-138 | 383 |
| MS.gene034838.t1 | MTR_1g058540 | 81.633 | 196 | 35 | 1 | 1 | 195 | 1 | 196 | 6.87e-100 | 287 |
| MS.gene034838.t1 | MTR_1g058570 | 88.205 | 195 | 22 | 1 | 1 | 195 | 1 | 194 | 1.32e-99 | 286 |
| MS.gene034838.t1 | MTR_2g086740 | 60.694 | 173 | 68 | 0 | 22 | 194 | 47 | 219 | 7.52e-59 | 184 |
| MS.gene034838.t1 | MTR_7g011090 | 52.941 | 170 | 80 | 0 | 26 | 195 | 17 | 186 | 4.19e-56 | 176 |
| MS.gene034838.t1 | MTR_4g081880 | 42.932 | 191 | 103 | 2 | 4 | 194 | 2 | 186 | 6.88e-51 | 162 |
| MS.gene034838.t1 | MTR_5g041900 | 48.503 | 167 | 86 | 0 | 29 | 195 | 20 | 186 | 5.07e-45 | 147 |
| MS.gene034838.t1 | MTR_2g086740 | 63.462 | 104 | 38 | 0 | 22 | 125 | 47 | 150 | 2.23e-34 | 119 |
| MS.gene034838.t1 | MTR_0004s0210 | 37.419 | 155 | 84 | 4 | 36 | 188 | 7 | 150 | 5.21e-21 | 85.5 |
| MS.gene034838.t1 | MTR_0097s0070 | 33.898 | 118 | 76 | 2 | 72 | 188 | 69 | 185 | 6.44e-14 | 67.4 |
| MS.gene034838.t1 | MTR_4g118800 | 30.833 | 120 | 82 | 1 | 74 | 192 | 62 | 181 | 1.86e-12 | 63.2 |
| MS.gene034838.t1 | MTR_8g089300 | 29.487 | 156 | 101 | 3 | 41 | 188 | 30 | 184 | 1.90e-12 | 63.5 |
| MS.gene034838.t1 | MTR_7g108130 | 27.742 | 155 | 109 | 2 | 36 | 188 | 28 | 181 | 2.47e-12 | 63.2 |
| MS.gene034838.t1 | MTR_3g064310 | 32.203 | 118 | 77 | 2 | 75 | 192 | 77 | 191 | 3.08e-12 | 63.2 |
| MS.gene034838.t1 | MTR_5g076480 | 34.483 | 116 | 72 | 3 | 75 | 189 | 69 | 181 | 4.25e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene034838.t1 | AT2G36100 | 56.863 | 204 | 76 | 3 | 2 | 195 | 5 | 206 | 1.27e-70 | 213 |
| MS.gene034838.t1 | AT3G11550 | 62.189 | 201 | 68 | 3 | 2 | 195 | 5 | 204 | 3.20e-64 | 197 |
| MS.gene034838.t1 | AT5G06200 | 61.616 | 198 | 70 | 3 | 2 | 194 | 5 | 201 | 1.02e-63 | 196 |
| MS.gene034838.t1 | AT2G27370 | 51.724 | 203 | 83 | 2 | 7 | 194 | 17 | 219 | 1.99e-62 | 193 |
| MS.gene034838.t1 | AT5G15290 | 44.828 | 174 | 86 | 1 | 2 | 175 | 3 | 166 | 1.91e-49 | 159 |
| MS.gene034838.t1 | AT1G14160 | 41.212 | 165 | 97 | 0 | 31 | 195 | 45 | 209 | 3.99e-44 | 146 |
| MS.gene034838.t1 | AT1G03700 | 32.886 | 149 | 91 | 2 | 40 | 188 | 12 | 151 | 2.50e-19 | 81.3 |
| MS.gene034838.t1 | AT1G17200 | 27.660 | 188 | 124 | 4 | 9 | 192 | 2 | 181 | 2.00e-16 | 74.3 |
| MS.gene034838.t1 | AT4G03540 | 33.058 | 121 | 72 | 2 | 56 | 176 | 28 | 139 | 2.31e-15 | 70.9 |
| MS.gene034838.t1 | AT5G44550 | 33.951 | 162 | 95 | 4 | 41 | 194 | 22 | 179 | 2.68e-15 | 71.2 |
| MS.gene034838.t1 | AT3G06390 | 30.075 | 133 | 88 | 2 | 37 | 164 | 37 | 169 | 2.78e-13 | 65.9 |
| MS.gene034838.t1 | AT4G15630 | 26.601 | 203 | 118 | 6 | 2 | 192 | 6 | 189 | 7.74e-12 | 61.6 |
| MS.gene034838.t1 | AT4G15610 | 26.144 | 153 | 107 | 3 | 28 | 175 | 20 | 171 | 8.02e-12 | 61.6 |
Find 40 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCATCCTCCTGGCCTTGC+AGG | 0.176057 | 1.4:+48597094 | None:intergenic |
| TGATTCTTGGATATCAATTT+TGG | 0.215560 | 1.4:+48597156 | None:intergenic |
| CTAGCCATTTGCAACCAATT+TGG | 0.242258 | 1.4:-48596277 | MS.gene034838:CDS |
| GTGATGTCTGCTTTGGCTAT+TGG | 0.247792 | 1.4:-48596181 | MS.gene034838:CDS |
| TGGTATCTTTGCTTGATTCT+TGG | 0.263645 | 1.4:+48597143 | None:intergenic |
| GGCTATAGTGGCTGGCTATT+TGG | 0.277845 | 1.4:-48596740 | MS.gene034838:CDS |
| TGATGTCTGCTTTGGCTATT+GGG | 0.288501 | 1.4:-48596180 | MS.gene034838:CDS |
| CCTTAAAATGAAATCCATTA+TGG | 0.301071 | 1.4:+48597060 | None:intergenic |
| TACCAAAGGAAAAGCTGTTT+TGG | 0.315492 | 1.4:-48597125 | MS.gene034838:CDS |
| ATGATGGCTACTATAGAAAA+AGG | 0.341980 | 1.4:+48596706 | None:intergenic |
| AAGGCCAGGAGGATGGAAAA+AGG | 0.353235 | 1.4:-48597089 | MS.gene034838:CDS |
| TCTTATTGCAATGGCTATAG+TGG | 0.369985 | 1.4:-48596752 | MS.gene034838:CDS |
| TCTTGCCGCTGCCGCCTCGA+TGG | 0.384071 | 1.4:-48597017 | MS.gene034838:CDS |
| AGCTGCTTCCATAGTTTACT+TGG | 0.385601 | 1.4:-48596329 | MS.gene034838:CDS |
| CTTGCCGCTGCCGCCTCGAT+GGG | 0.402133 | 1.4:-48597016 | MS.gene034838:CDS |
| GTTGGTTGTGATGTCTGCTT+TGG | 0.406589 | 1.4:-48596188 | MS.gene034838:CDS |
| GTGCACAGAAATCTCCAAAT+TGG | 0.409853 | 1.4:+48596263 | None:intergenic |
| ATGGAAATCAAGACACAAAC+TGG | 0.414877 | 1.4:-48596300 | MS.gene034838:CDS |
| AGCGGCAAGAGCTGCTGCTA+TGG | 0.432197 | 1.4:+48597030 | None:intergenic |
| ATCTCCAAATTGGTTGCAAA+TGG | 0.445100 | 1.4:+48596273 | None:intergenic |
| GTTGCAGCACCTGCAAGGCC+AGG | 0.457764 | 1.4:-48597103 | MS.gene034838:CDS |
| CACCTGCAAGGCCAGGAGGA+TGG | 0.475215 | 1.4:-48597096 | MS.gene034838:CDS |
| ATTGTGTGCCAAGTAAACTA+TGG | 0.493367 | 1.4:+48596321 | None:intergenic |
| AATGTTACATACTGGAATGC+AGG | 0.494232 | 1.4:+48596926 | None:intergenic |
| AAGCCTTGGTCCAGTTGCAT+GGG | 0.497314 | 1.4:+48596678 | None:intergenic |
| TGTTCCCATCGAGGCGGCAG+CGG | 0.517932 | 1.4:+48597012 | None:intergenic |
| AAAGCCTTGGTCCAGTTGCA+TGG | 0.518863 | 1.4:+48596677 | None:intergenic |
| ATTGCAATGGCTATAGTGGC+TGG | 0.542648 | 1.4:-48596748 | MS.gene034838:CDS |
| ATTGCAGGTATTTCTTACAC+TGG | 0.547240 | 1.4:-48596371 | MS.gene034838:intron |
| ATTGAGATCTTACAGTGTCT+AGG | 0.588191 | 1.4:+48596644 | None:intergenic |
| TGTGGCATCATTTATTACTG+TGG | 0.599611 | 1.4:-48596224 | MS.gene034838:CDS |
| ACAGACAAGTGGTGCAGTTG+TGG | 0.601968 | 1.4:-48596242 | MS.gene034838:CDS |
| AGCCTTGGTCCAGTTGCATG+GGG | 0.616293 | 1.4:+48596679 | None:intergenic |
| ATAGTTTACTTGGCACACAA+TGG | 0.619739 | 1.4:-48596319 | MS.gene034838:CDS |
| GCAGCACCTGCAAGGCCAGG+AGG | 0.636977 | 1.4:-48597100 | MS.gene034838:CDS |
| GAATCAAGCAAAGATACCAA+AGG | 0.648211 | 1.4:-48597139 | MS.gene034838:CDS |
| ATCACTTGTTCCCATCGAGG+CGG | 0.653937 | 1.4:+48597006 | None:intergenic |
| TTGATCACTTGTTCCCATCG+AGG | 0.661241 | 1.4:+48597003 | None:intergenic |
| GATTTCTGTGCACAGACAAG+TGG | 0.683686 | 1.4:-48596253 | MS.gene034838:CDS |
| GCCTTGGTCCAGTTGCATGG+GGG | 0.707472 | 1.4:+48596680 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CTCAAATATAAGTAAAAGTT+AGG | - | chr1.4:48596769-48596788 | MS.gene034838:intron | 20.0% |
| !!! | GATGTATTTAATTCAACTTT+TGG | + | chr1.4:48596819-48596838 | None:intergenic | 20.0% |
| !!! | TTAGTTATGATTTTTCTTTG+TGG | - | chr1.4:48596932-48596951 | MS.gene034838:intron | 20.0% |
| ! | CCTTAAAATGAAATCCATTA+TGG | + | chr1.4:48596277-48596296 | None:intergenic | 25.0% |
| ! | TTCTAAGTCACATATAAGAT+CGG | - | chr1.4:48596453-48596472 | MS.gene034838:intron | 25.0% |
| !! | TGATTCTTGGATATCAATTT+TGG | + | chr1.4:48596181-48596200 | None:intergenic | 25.0% |
| !!! | ATGGATTTCATTTTAAGGTT+AGG | - | chr1.4:48596279-48596298 | MS.gene034838:CDS | 25.0% |
| !!! | CCATAATGGATTTCATTTTA+AGG | - | chr1.4:48596274-48596293 | MS.gene034838:CDS | 25.0% |
| !!! | CTTTTACTTATATTTGAGAC+TGG | + | chr1.4:48596767-48596786 | None:intergenic | 25.0% |
| !!! | TCAACAAAAGTTGATGTATT+TGG | - | chr1.4:48596792-48596811 | MS.gene034838:intron | 25.0% |
| ACTTAATGCTTGAAAAATGC+AGG | - | chr1.4:48596554-48596573 | MS.gene034838:intron | 30.0% | |
| ATGATGGCTACTATAGAAAA+AGG | + | chr1.4:48596631-48596650 | None:intergenic | 30.0% | |
| TAGATGTTTACTACTCTCTT+CGG | + | chr1.4:48596878-48596897 | None:intergenic | 30.0% | |
| ! | TACTTATATTTGAGACTGGA+GGG | + | chr1.4:48596763-48596782 | None:intergenic | 30.0% |
| ! | TTACTTATATTTGAGACTGG+AGG | + | chr1.4:48596764-48596783 | None:intergenic | 30.0% |
| !!! | CAGGTTTTTTCTTATTGCAA+TGG | - | chr1.4:48596573-48596592 | MS.gene034838:intron | 30.0% |
| !!! | GAGTTTTGAATGTTACATAC+TGG | + | chr1.4:48596419-48596438 | None:intergenic | 30.0% |
| !!! | TGTGGTTGTTTTAATCTTGT+TGG | - | chr1.4:48597128-48597147 | MS.gene034838:CDS | 30.0% |
| AATGTTACATACTGGAATGC+AGG | + | chr1.4:48596411-48596430 | None:intergenic | 35.0% | |
| ATAGTTTACTTGGCACACAA+TGG | - | chr1.4:48597015-48597034 | MS.gene034838:CDS | 35.0% | |
| ATCTCCAAATTGGTTGCAAA+TGG | + | chr1.4:48597064-48597083 | None:intergenic | 35.0% | |
| ATGGAAATCAAGACACAAAC+TGG | - | chr1.4:48597034-48597053 | MS.gene034838:CDS | 35.0% | |
| ATTGAGATCTTACAGTGTCT+AGG | + | chr1.4:48596693-48596712 | None:intergenic | 35.0% | |
| ATTGCAGGTATTTCTTACAC+TGG | - | chr1.4:48596963-48596982 | MS.gene034838:CDS | 35.0% | |
| ATTGTGTGCCAAGTAAACTA+TGG | + | chr1.4:48597016-48597035 | None:intergenic | 35.0% | |
| GAATCAAGCAAAGATACCAA+AGG | - | chr1.4:48596195-48596214 | MS.gene034838:CDS | 35.0% | |
| TCTTATTGCAATGGCTATAG+TGG | - | chr1.4:48596582-48596601 | MS.gene034838:intron | 35.0% | |
| TGGTATCTTTGCTTGATTCT+TGG | + | chr1.4:48596194-48596213 | None:intergenic | 35.0% | |
| TGTGGCATCATTTATTACTG+TGG | - | chr1.4:48597110-48597129 | MS.gene034838:CDS | 35.0% | |
| TTTGTGGTGCTTATAATTGC+AGG | - | chr1.4:48596948-48596967 | MS.gene034838:CDS | 35.0% | |
| ! | AACCAAAACAGCTTTTCCTT+TGG | + | chr1.4:48596214-48596233 | None:intergenic | 35.0% |
| ! | ACTTATATTTGAGACTGGAG+GGG | + | chr1.4:48596762-48596781 | None:intergenic | 35.0% |
| ! | TACCAAAGGAAAAGCTGTTT+TGG | - | chr1.4:48596209-48596228 | MS.gene034838:CDS | 35.0% |
| AGCTGCTTCCATAGTTTACT+TGG | - | chr1.4:48597005-48597024 | MS.gene034838:CDS | 40.0% | |
| AGGATGATGAGAAAAAGCCT+TGG | + | chr1.4:48596673-48596692 | None:intergenic | 40.0% | |
| CTAGCCATTTGCAACCAATT+TGG | - | chr1.4:48597057-48597076 | MS.gene034838:CDS | 40.0% | |
| GTGCACAGAAATCTCCAAAT+TGG | + | chr1.4:48597074-48597093 | None:intergenic | 40.0% | |
| TGATGTCTGCTTTGGCTATT+GGG | - | chr1.4:48597154-48597173 | MS.gene034838:CDS | 40.0% | |
| ! | CTTATATTTGAGACTGGAGG+GGG | + | chr1.4:48596761-48596780 | None:intergenic | 40.0% |
| ! | GAAAAAGGGCATAGCCATAA+TGG | - | chr1.4:48596260-48596279 | MS.gene034838:CDS | 40.0% |
| ATTGCAATGGCTATAGTGGC+TGG | - | chr1.4:48596586-48596605 | MS.gene034838:intron | 45.0% | |
| GATTTCTGTGCACAGACAAG+TGG | - | chr1.4:48597081-48597100 | MS.gene034838:CDS | 45.0% | |
| GTGATGTCTGCTTTGGCTAT+TGG | - | chr1.4:48597153-48597172 | MS.gene034838:CDS | 45.0% | |
| GTTGGTTGTGATGTCTGCTT+TGG | - | chr1.4:48597146-48597165 | MS.gene034838:CDS | 45.0% | |
| TTGATCACTTGTTCCCATCG+AGG | + | chr1.4:48596334-48596353 | None:intergenic | 45.0% | |
| AAAGCCTTGGTCCAGTTGCA+TGG | + | chr1.4:48596660-48596679 | None:intergenic | 50.0% | |
| AAGCCTTGGTCCAGTTGCAT+GGG | + | chr1.4:48596659-48596678 | None:intergenic | 50.0% | |
| AAGGCCAGGAGGATGGAAAA+AGG | - | chr1.4:48596245-48596264 | MS.gene034838:CDS | 50.0% | |
| ACAGACAAGTGGTGCAGTTG+TGG | - | chr1.4:48597092-48597111 | MS.gene034838:CDS | 50.0% | |
| AGGCCAGGAGGATGGAAAAA+GGG | - | chr1.4:48596246-48596265 | MS.gene034838:CDS | 50.0% | |
| ATCACTTGTTCCCATCGAGG+CGG | + | chr1.4:48596331-48596350 | None:intergenic | 50.0% | |
| ! | ATGCCCTTTTTCCATCCTCC+TGG | + | chr1.4:48596252-48596271 | None:intergenic | 50.0% |
| ! | GGCTATAGTGGCTGGCTATT+TGG | - | chr1.4:48596594-48596613 | MS.gene034838:intron | 50.0% |
| !! | TTTTGGTTGCAGCACCTGCA+AGG | - | chr1.4:48596226-48596245 | MS.gene034838:CDS | 50.0% |
| AGCCTTGGTCCAGTTGCATG+GGG | + | chr1.4:48596658-48596677 | None:intergenic | 55.0% | |
| ATCATACGCCCCCATGCAAC+TGG | - | chr1.4:48596646-48596665 | MS.gene034838:intron | 55.0% | |
| ! | AGTTGCATGGGGGCGTATGA+TGG | + | chr1.4:48596647-48596666 | None:intergenic | 55.0% |
| AGCGGCAAGAGCTGCTGCTA+TGG | + | chr1.4:48596307-48596326 | None:intergenic | 60.0% | |
| GCCTTGGTCCAGTTGCATGG+GGG | + | chr1.4:48596657-48596676 | None:intergenic | 60.0% | |
| !! | TTCCATCCTCCTGGCCTTGC+AGG | + | chr1.4:48596243-48596262 | None:intergenic | 60.0% |
| CACCTGCAAGGCCAGGAGGA+TGG | - | chr1.4:48596238-48596257 | MS.gene034838:CDS | 65.0% | |
| GCCCCCATGCAACTGGACCA+AGG | - | chr1.4:48596653-48596672 | MS.gene034838:intron | 65.0% | |
| GTTGCAGCACCTGCAAGGCC+AGG | - | chr1.4:48596231-48596250 | MS.gene034838:CDS | 65.0% | |
| TGTTCCCATCGAGGCGGCAG+CGG | + | chr1.4:48596325-48596344 | None:intergenic | 65.0% | |
| CTTGCCGCTGCCGCCTCGAT+GGG | - | chr1.4:48596318-48596337 | MS.gene034838:CDS | 70.0% | |
| GCAGCACCTGCAAGGCCAGG+AGG | - | chr1.4:48596234-48596253 | MS.gene034838:CDS | 70.0% | |
| TCTTGCCGCTGCCGCCTCGA+TGG | - | chr1.4:48596317-48596336 | MS.gene034838:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 48596171 | 48597185 | 48596171 | ID=MS.gene034838 |
| chr1.4 | mRNA | 48596171 | 48597185 | 48596171 | ID=MS.gene034838.t1;Parent=MS.gene034838 |
| chr1.4 | exon | 48596938 | 48597185 | 48596938 | ID=MS.gene034838.t1.exon1;Parent=MS.gene034838.t1 |
| chr1.4 | CDS | 48596938 | 48597185 | 48596938 | ID=cds.MS.gene034838.t1;Parent=MS.gene034838.t1 |
| chr1.4 | exon | 48596657 | 48596780 | 48596657 | ID=MS.gene034838.t1.exon2;Parent=MS.gene034838.t1 |
| chr1.4 | CDS | 48596657 | 48596780 | 48596657 | ID=cds.MS.gene034838.t1;Parent=MS.gene034838.t1 |
| chr1.4 | exon | 48596171 | 48596386 | 48596171 | ID=MS.gene034838.t1.exon3;Parent=MS.gene034838.t1 |
| chr1.4 | CDS | 48596171 | 48596386 | 48596171 | ID=cds.MS.gene034838.t1;Parent=MS.gene034838.t1 |
| Gene Sequence |
| Protein sequence |
