AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene035199

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035199.t1 MTR_7g098430 98.063 826 16 0 1 826 1 826 0.0 1674
MS.gene035199.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 2.73e-134 429
MS.gene035199.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 2.85e-134 429
MS.gene035199.t1 MTR_8g088050 39.753 566 322 6 158 711 18 576 2.91e-134 429
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene035199.t1 AT1G04080 64.915 704 225 9 109 795 37 735 0.0 913
MS.gene035199.t1 AT1G04080 64.915 704 225 9 109 795 37 735 0.0 913
MS.gene035199.t1 AT1G04080 59.396 761 228 12 109 795 37 790 0.0 866
MS.gene035199.t1 AT1G04080 64.845 549 174 7 261 795 6 549 0.0 702
MS.gene035199.t1 AT1G04080 63.724 521 170 7 289 795 1 516 0.0 650
MS.gene035199.t1 AT5G46400 41.392 546 296 9 154 687 30 563 3.68e-128 409
MS.gene035199.t1 AT5G46400 40.000 325 188 4 364 687 25 343 3.18e-66 236
MS.gene035199.t1 AT5G46400 40.000 325 188 4 364 687 25 343 3.18e-66 236
MS.gene035199.t1 AT4G24270 25.392 319 185 14 162 465 59 339 7.97e-11 66.2
MS.gene035199.t1 AT4G24270 25.392 319 185 14 162 465 59 339 8.10e-11 66.2

Find 209 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTATGCATTTACTTATTC+TGG 0.134991 4.2:+21193377 MS.gene035199:CDS
GCTTCTCCTGCAGCCTCGGC+TGG 0.142915 4.2:-21193332 None:intergenic
TTATTCTGTCATTAGGCTTC+TGG 0.145350 4.2:+21198749 MS.gene035199:intron
TATCGATCCAATTGCTGATT+TGG 0.218895 4.2:-21196021 None:intergenic
CTTGCTACACCAGGGTTCTC+TGG 0.220827 4.2:-21199087 None:intergenic
ATTTGGATTCTCTAATATTC+TGG 0.228707 4.2:-21196004 None:intergenic
TGAGCTTGTAGTACAGAATT+AGG 0.232263 4.2:-21193428 None:intergenic
CAGAATTCCCTTTGTGTTAT+GGG 0.232733 4.2:+21195086 MS.gene035199:CDS
ATTATCTTTCGTTTCCTCTT+TGG 0.236191 4.2:+21195920 MS.gene035199:CDS
ACGTACGACCTCTTAATATT+GGG 0.271534 4.2:+21196501 MS.gene035199:CDS
TGAAAACTGGCATAATTATC+TGG 0.271539 4.2:+21196529 MS.gene035199:CDS
CTTATACATCTCTTCTCTAA+TGG 0.273988 4.2:-21196414 None:intergenic
CCAAACTGTTCTTGCCAAAC+AGG 0.276139 4.2:+21199304 MS.gene035199:CDS
GATACAGTTGGACTTGAAAA+TGG 0.279480 4.2:+21193578 MS.gene035199:CDS
CACGTACGACCTCTTAATAT+TGG 0.285155 4.2:+21196500 MS.gene035199:CDS
CTTCTCCTGCAGCCTCGGCT+GGG 0.286804 4.2:-21193331 None:intergenic
TTATCTTTCGTTTCCTCTTT+GGG 0.287270 4.2:+21195921 MS.gene035199:CDS
GCTGCTGCTCTGCCCAATTC+AGG 0.288615 4.2:+21193356 MS.gene035199:CDS
GCAGAATTCCCTTTGTGTTA+TGG 0.289100 4.2:+21195085 MS.gene035199:CDS
GCTGACCATTCATCTGTAAA+TGG 0.289348 4.2:+21193548 MS.gene035199:CDS
CGGCTGCAGGTTGCTGTGTC+GGG 0.291007 4.2:-21200672 None:intergenic
AATACAGCACTTGCAGGTTT+AGG 0.295664 4.2:-21196359 None:intergenic
CCTTGTGCTTGAGTCGTTGC+TGG 0.297776 4.2:-21199492 None:intergenic
AGTAAATGCATAGCCTGAAT+TGG 0.309418 4.2:-21193369 None:intergenic
GTATTCTCGATTTGTTTATT+TGG 0.319345 4.2:+21198306 MS.gene035199:CDS
TGCTGTGTCGGGGCTGCAAC+AGG 0.320209 4.2:-21200661 None:intergenic
GTTCCACAAAGCACTGCTTA+TGG 0.321030 4.2:+21200125 MS.gene035199:CDS
TCCCTTTGTGTTATGGGTAT+TGG 0.321785 4.2:+21195092 MS.gene035199:CDS
TATTCTGTCATTAGGCTTCT+GGG 0.323007 4.2:+21198750 MS.gene035199:intron
TATGGGGCTTATGCTGGATA+TGG 0.331066 4.2:+21200068 MS.gene035199:CDS
TATCCAGTACTCGGGATAAT+TGG 0.333216 4.2:-21197115 None:intergenic
GCCGTTCCTCAGGCATATTA+TGG 0.342067 4.2:+21200691 MS.gene035199:CDS
TGCCTTCTCTTTGTATGAAC+AGG 0.345610 4.2:+21198225 MS.gene035199:CDS
ATCGACCTCTTTCAGAATTA+CGG 0.355782 4.2:+21196240 MS.gene035199:CDS
CCTAACCAATGGCCTAACTA+TGG 0.364535 4.2:+21199450 MS.gene035199:CDS
GGCCATTGGTTAGGTCCATT+TGG 0.367818 4.2:-21199441 None:intergenic
TTCCACAAAGCACTGCTTAT+GGG 0.376637 4.2:+21200126 MS.gene035199:CDS
CCTGTTTGGCAAGAACAGTT+TGG 0.377400 4.2:-21199304 None:intergenic
TACACCAGGGTTCTCTGGGT+TGG 0.381425 4.2:-21199082 None:intergenic
TATGGAAGCTAGGGAAAGTA+TGG 0.382672 4.2:+21197152 MS.gene035199:CDS
TGCTGCTGGGTGTAGCCAGC+AGG 0.383369 4.2:-21199525 None:intergenic
TCTGTTAATACAGCACTTGC+AGG 0.384276 4.2:-21196365 None:intergenic
ATGAGTACATGCAGCAAGAC+TGG 0.385484 4.2:+21195959 MS.gene035199:CDS
CAATGTCAACTCGCTTTGGC+TGG 0.386893 4.2:-21199023 None:intergenic
AATAATCAGGCATGCCAATA+TGG 0.389365 4.2:+21198094 MS.gene035199:CDS
CCTGCAGCCTCGGCTGGGGC+AGG 0.390779 4.2:-21193326 None:intergenic
GAGATGTTCAATCGATCAAA+AGG 0.399956 4.2:+21199265 MS.gene035199:CDS
AGCAGCTTCTCCTGCAGCCT+CGG 0.403142 4.2:-21193336 None:intergenic
GATGAAGCTGCTGCAGTGGC+AGG 0.405134 4.2:+21196269 MS.gene035199:CDS
ACACCAGGGTTCTCTGGGTT+GGG 0.411863 4.2:-21199081 None:intergenic
TGAGGCTGTACTCCATAGTT+AGG 0.412997 4.2:-21199462 None:intergenic
ATACAGGCTGCCGCTTCCTA+TGG 0.418623 4.2:+21200050 MS.gene035199:intron
AACTCCGACAAGCCCCTGTT+TGG 0.419402 4.2:-21199318 None:intergenic
TTCTCCTGCAGCCTCGGCTG+GGG 0.420645 4.2:-21193330 None:intergenic
TTGCGCTCGGTTCAAGGAGC+AGG 0.422648 4.2:+21197998 MS.gene035199:CDS
GGAGAATTATCTGCACCATC+AGG 0.426487 4.2:-21196338 None:intergenic
AATGTCAACTCGCTTTGGCT+GGG 0.427195 4.2:-21199022 None:intergenic
TATTACCTACGAACTGTGTC+TGG 0.428305 4.2:-21195256 None:intergenic
CATGATATTGCAGGGGAAGT+TGG 0.428308 4.2:+21198198 MS.gene035199:intron
ACGGCTGCAGGTTGCTGTGT+CGG 0.430705 4.2:-21200673 None:intergenic
TTCACCTGTGCAGGATATGC+AGG 0.431301 4.2:-21200158 None:intergenic
TTAGCACCAACCTTGGGAAC+AGG 0.431376 4.2:-21193299 None:intergenic
TAAATGGTGTTGCAGCTTCA+AGG 0.434451 4.2:+21196205 MS.gene035199:intron
TACAGGCTGCCGCTTCCTAT+GGG 0.435003 4.2:+21200051 MS.gene035199:intron
CCTGCCCCAGCCGAGGCTGC+AGG 0.435556 4.2:+21193326 MS.gene035199:CDS
GCATTTACTTATTCTGGTGA+TGG 0.435692 4.2:+21193383 MS.gene035199:CDS
TACCAATCGATGTTCCATAT+TGG 0.436137 4.2:-21198108 None:intergenic
GATGGGAATGCTTATACTGC+TGG 0.441620 4.2:+21193401 MS.gene035199:CDS
CCGTTCCTCAGGCATATTAT+GGG 0.443253 4.2:+21200692 MS.gene035199:CDS
AATTGGATCGATATTTCAAC+AGG 0.444059 4.2:+21196031 MS.gene035199:CDS
AAAACCAATTTACCTGCTGC+TGG 0.444538 4.2:-21199539 None:intergenic
GTCTTCTTGAAGCAATAATC+AGG 0.448175 4.2:+21198081 MS.gene035199:CDS
AGAGAATCCAAATCAGCAAT+TGG 0.448924 4.2:+21196014 MS.gene035199:CDS
TTGCTACACCAGGGTTCTCT+GGG 0.452718 4.2:-21199086 None:intergenic
GTTCACACTGAAATTTCTCC+AGG 0.453659 4.2:+21198059 MS.gene035199:CDS
CATTGACTTTCTAGAGTCAC+TGG 0.456681 4.2:+21199041 MS.gene035199:CDS
GATATGCACCCACTACAGAT+TGG 0.457141 4.2:-21199419 None:intergenic
GCCCCATAAGCAGTGCTTTG+TGG 0.457630 4.2:-21200128 None:intergenic
ATATTGGGGAGCTTGAAAAC+TGG 0.459736 4.2:+21196516 MS.gene035199:CDS
GCTGACCATGAGGCGCGCCT+TGG 0.460933 4.2:+21195124 MS.gene035199:CDS
GCTTCCTATGGGGCTTATGC+TGG 0.462340 4.2:+21200062 MS.gene035199:CDS
TCTGGTTGGAAAGGAAGCTC+TGG 0.463748 4.2:+21193508 MS.gene035199:CDS
CTTGCCAAACAGGGGCTTGT+CGG 0.464209 4.2:+21199314 MS.gene035199:CDS
GAGTTCGATTCTAAGATCAT+TGG 0.466817 4.2:+21196446 MS.gene035199:CDS
CATTTACTTATTCTGGTGAT+GGG 0.468008 4.2:+21193384 MS.gene035199:CDS
CACTTCCATTTACAGATGAA+TGG 0.468952 4.2:-21193553 None:intergenic
CCATAGTTAGGCCATTGGTT+AGG 0.469295 4.2:-21199450 None:intergenic
TGCTGGCTACACCCAGCAGC+AGG 0.471209 4.2:+21199527 MS.gene035199:CDS
TCGAATCCTGTTCCCAAGGT+TGG 0.472109 4.2:+21193293 MS.gene035199:CDS
AACGAGTCAAAGCAAGCTGC+TGG 0.475326 4.2:+21193464 MS.gene035199:CDS
TCAAGCTCCCCAATATTAAG+AGG 0.477060 4.2:-21196509 None:intergenic
GTTTATGAACGAGCTGTTCA+AGG 0.479117 4.2:+21195169 MS.gene035199:CDS
GAACGAGGATTAGCTTATGT+TGG 0.479715 4.2:+21195892 MS.gene035199:CDS
AGCTTCCTTTCCAACCAGAC+TGG 0.482155 4.2:-21193504 None:intergenic
TGCAAATAATGTTCTCGCTC+GGG 0.483808 4.2:+21197179 MS.gene035199:CDS
GTAAATGCATAGCCTGAATT+GGG 0.486099 4.2:-21193368 None:intergenic
ATGTCAACTCGCTTTGGCTG+GGG 0.492568 4.2:-21199021 None:intergenic
GTGCCAATTATCCCGAGTAC+TGG 0.494096 4.2:+21197112 MS.gene035199:CDS
AAGTCAATGTCAACTCGCTT+TGG 0.496185 4.2:-21199027 None:intergenic
CAAGGACAACAATGGCCTGC+TGG 0.496647 4.2:+21199510 MS.gene035199:CDS
GCCTAATGAGGATTCCAGTC+TGG 0.497327 4.2:+21193490 MS.gene035199:CDS
ATTAACAGAAGCAGAAGAGT+TGG 0.497386 4.2:+21196379 MS.gene035199:CDS
GGAACTGATGCAGGCAATTG+TGG 0.498199 4.2:-21200107 None:intergenic
TCCAATACCCATAACACAAA+GGG 0.499353 4.2:-21195093 None:intergenic
CAGGCCATTGCCATTGAGAA+AGG 0.499761 4.2:+21198244 MS.gene035199:CDS
TATGCCTGAGGAACGGCTGC+AGG 0.506298 4.2:-21200685 None:intergenic
GCAACCTGCAGCCGTTCCTC+AGG 0.509331 4.2:+21200681 MS.gene035199:CDS
AAGAGGTCGTACGTGAAAGT+AGG 0.516439 4.2:-21196492 None:intergenic
GGCTTCTGGGAATTCCGAAA+AGG 0.517187 4.2:+21198763 MS.gene035199:intron
GGTACTGACCAAGGTGTTGA+AGG 0.517289 4.2:+21196305 MS.gene035199:CDS
GAGCAGGCTGGAGATATTGT+TGG 0.520258 4.2:+21198014 MS.gene035199:CDS
CAGAGCCAAGGCGCGCCTCA+TGG 0.522186 4.2:-21195129 None:intergenic
ATATGCACCCACTACAGATT+GGG 0.523107 4.2:-21199418 None:intergenic
CGAGACAGAGAAGGTGGCAG+AGG 0.524685 4.2:+21194610 MS.gene035199:CDS
GGAACATTAGCACCAACCTT+GGG 0.526018 4.2:-21193305 None:intergenic
TGCCAATATGGAACATCGAT+TGG 0.531852 4.2:+21198106 MS.gene035199:CDS
TTGCAAATAATGTTCTCGCT+CGG 0.532036 4.2:+21197178 MS.gene035199:CDS
TCCTCCTGCATATCCTGCAC+AGG 0.532285 4.2:+21200154 MS.gene035199:CDS
CGCTTCGAATCCTGTTCCCA+AGG 0.532298 4.2:+21193289 MS.gene035199:CDS
GTGTTGCAGCTTCAAGGAGT+TGG 0.532516 4.2:+21196211 MS.gene035199:intron
GTCGTTGCTGGCCAAGTTTG+AGG 0.533522 4.2:-21199480 None:intergenic
ATGGAGTACAGCCTCAAACT+TGG 0.534256 4.2:+21199469 MS.gene035199:CDS
ATGGGAATGCTTATACTGCT+GGG 0.536210 4.2:+21193402 MS.gene035199:CDS
ACCAGACTGGAATCCTCATT+AGG 0.536266 4.2:-21193491 None:intergenic
AGGAACATTAGCACCAACCT+TGG 0.537288 4.2:-21193306 None:intergenic
AACATAGCGTATCCAGTACT+CGG 0.538429 4.2:-21197124 None:intergenic
TCTGTTGCACTTGCTACACC+AGG 0.538737 4.2:-21199096 None:intergenic
TCAATGTATTTATCCCAAAG+AGG 0.538798 4.2:-21195934 None:intergenic
ATATTCGCTATAAGCACCTA+TGG 0.541316 4.2:+21195229 MS.gene035199:CDS
GCTCGGTTCAAGGAGCAGGC+TGG 0.541555 4.2:+21198002 MS.gene035199:CDS
CAAACTGTTCTTGCCAAACA+GGG 0.544913 4.2:+21199305 MS.gene035199:CDS
AGCATAAGCCCCATAGGAAG+CGG 0.546523 4.2:-21200060 None:intergenic
GCTGTCCGTAATTCTGAAAG+AGG 0.548224 4.2:-21196245 None:intergenic
GGCTGCAGGTTGCTGTGTCG+GGG 0.548629 4.2:-21200671 None:intergenic
GTGGCAGGTGTTGTTTCAGA+GGG 0.550122 4.2:+21196284 MS.gene035199:CDS
GTACTCCATAGTTAGGCCAT+TGG 0.550599 4.2:-21199455 None:intergenic
AATGCATCAGAGAATGTTGA+TGG 0.550820 4.2:+21193602 MS.gene035199:CDS
TCCACAAAGCACTGCTTATG+GGG 0.553188 4.2:+21200127 MS.gene035199:CDS
AGTGGCAGGTGTTGTTTCAG+AGG 0.553746 4.2:+21196283 MS.gene035199:CDS
GTGCTTTGTGGAACTGATGC+AGG 0.555027 4.2:-21200116 None:intergenic
AAACAACTCACAGATGCTTA+CGG 0.557128 4.2:+21193638 MS.gene035199:CDS
AGCTGATGAAGCTGCTGCAG+TGG 0.558284 4.2:+21196265 MS.gene035199:CDS
GGTGTAAATCATTGCAAGGC+GGG 0.558474 4.2:-21195983 None:intergenic
GGGGCTTATGCTGGATATGG+AGG 0.560147 4.2:+21200071 MS.gene035199:CDS
AACTGTGTCTGGATCTCCAT+AGG 0.560352 4.2:-21195245 None:intergenic
TTTATGAACGAGCTGTTCAA+GGG 0.561629 4.2:+21195170 MS.gene035199:CDS
ATTATTGCTTCAAGAAGACC+TGG 0.562102 4.2:-21198077 None:intergenic
AATGAGGATTCCAGTCTGGT+TGG 0.562814 4.2:+21193494 MS.gene035199:CDS
AGAGGTCGTACGTGAAAGTA+GGG 0.564513 4.2:-21196491 None:intergenic
GGAAGCTCTGGACTCTGCTA+TGG 0.564721 4.2:+21193520 MS.gene035199:CDS
ATCACCTGCCCAATCTGTAG+TGG 0.565221 4.2:+21199410 MS.gene035199:CDS
AGATGTTCAATCGATCAAAA+GGG 0.566435 4.2:+21199266 MS.gene035199:CDS
GTAGTGGGTGCATATCCAAA+TGG 0.568266 4.2:+21199426 MS.gene035199:CDS
GGATTCCAGTCTGGTTGGAA+AGG 0.569228 4.2:+21193499 MS.gene035199:CDS
TTTGATTGACGAGACAGAGA+AGG 0.570064 4.2:+21194601 MS.gene035199:CDS
AAACCAATTTACCTGCTGCT+GGG 0.571184 4.2:-21199538 None:intergenic
TCAATCGATCAAAAGGGTTG+AGG 0.572118 4.2:+21199272 MS.gene035199:CDS
GATATTGCAGGGGAAGTTGG+AGG 0.576699 4.2:+21198201 MS.gene035199:intron
TAATGTTCCTGCCCCAGCCG+AGG 0.579939 4.2:+21193319 MS.gene035199:CDS
AAGAGAAGGCGACTTGAGCA+AGG 0.580408 4.2:+21196562 MS.gene035199:CDS
TGCAGGATATGCAGGAGGAT+AGG 0.581110 4.2:-21200150 None:intergenic
TTCTGGTGTAAATCATTGCA+AGG 0.581222 4.2:-21195987 None:intergenic
GAATACTGAGCAAACAACAT+TGG 0.582577 4.2:-21198289 None:intergenic
GAACTGATGCAGGCAATTGT+GGG 0.582647 4.2:-21200106 None:intergenic
AGCTGCTGGAGTGCCTAATG+AGG 0.586064 4.2:+21193478 MS.gene035199:CDS
TGCTGCTGAGACGTATCCAT+CGG 0.586175 4.2:+21193250 MS.gene035199:CDS
AATGGAAGTGTTGATACAGT+TGG 0.591174 4.2:+21193566 MS.gene035199:CDS
CCCATAATATGCCTGAGGAA+CGG 0.592953 4.2:-21200692 None:intergenic
TGAGTACATGCAGCAAGACT+GGG 0.596076 4.2:+21195960 MS.gene035199:CDS
CAACTTTATCAGCAGAGCCA+AGG 0.596207 4.2:-21195141 None:intergenic
CGTACGACCTCTTAATATTG+GGG 0.599762 4.2:+21196502 MS.gene035199:CDS
TTACATATTCAGTGGACATG+TGG 0.600677 4.2:+21195194 MS.gene035199:CDS
ATATCCAGCATAAGCCCCAT+AGG 0.601936 4.2:-21200066 None:intergenic
ACTGTCAAAGCCACTACTTG+AGG 0.604117 4.2:+21198823 MS.gene035199:CDS
AGCAAGTGCAACAGAGCGCG+AGG 0.605392 4.2:+21199104 MS.gene035199:CDS
TCAAGGGGTTACATATTCAG+TGG 0.607137 4.2:+21195186 MS.gene035199:CDS
GCACCCACTACAGATTGGGC+AGG 0.607563 4.2:-21199414 None:intergenic
TCACCTGCCCAATCTGTAGT+GGG 0.612384 4.2:+21199411 MS.gene035199:CDS
TTCCAATACCCATAACACAA+AGG 0.612695 4.2:-21195094 None:intergenic
AACACAAAGGGAATTCTGCT+AGG 0.613157 4.2:-21195081 None:intergenic
TATGGGAGATAGTGAAACAA+TGG 0.615550 4.2:+21193214 None:intergenic
TGACCAAGGTGTTGAAGGAG+AGG 0.619456 4.2:+21196310 MS.gene035199:CDS
GTGTTGTTGTAAGCATCCGA+TGG 0.622239 4.2:-21193266 None:intergenic
ACATAGCGTATCCAGTACTC+GGG 0.622860 4.2:-21197123 None:intergenic
ATCCAAATGGACCTAACCAA+TGG 0.623577 4.2:+21199439 MS.gene035199:CDS
ATCAGAGAATGTTGATGGGT+CGG 0.627186 4.2:+21193607 MS.gene035199:CDS
TTGAGACAAACCTCAAGTAG+TGG 0.627545 4.2:-21198833 None:intergenic
CTGCTGAAGAAGATCGATTG+TGG 0.627715 4.2:+21194534 MS.gene035199:CDS
GGCCTGTTCATACAAAGAGA+AGG 0.629259 4.2:-21198227 None:intergenic
GATTGACGAGACAGAGAAGG+TGG 0.630394 4.2:+21194604 MS.gene035199:CDS
ACAGGCTGCCGCTTCCTATG+GGG 0.637522 4.2:+21200052 MS.gene035199:intron
GTACTCGGGATAATTGGCAC+AGG 0.638207 4.2:-21197109 None:intergenic
CTCAAGCACAAGGACAACAA+TGG 0.638781 4.2:+21199502 MS.gene035199:CDS
GTTTCAGAGGGTACTGACCA+AGG 0.640522 4.2:+21196296 MS.gene035199:CDS
GAAGGAGAGGTTCATCCTGA+TGG 0.646457 4.2:+21196323 MS.gene035199:CDS
GAACCTCTCCTTCAACACCT+TGG 0.647061 4.2:-21196313 None:intergenic
ATGCATCAGAGAATGTTGAT+GGG 0.647426 4.2:+21193603 MS.gene035199:CDS
CTGTTGCACTTGCTACACCA+GGG 0.651331 4.2:-21199095 None:intergenic
ACCTGTGCAGGATATGCAGG+AGG 0.651957 4.2:-21200155 None:intergenic
AAACTGTTCTTGCCAAACAG+GGG 0.652207 4.2:+21199306 MS.gene035199:CDS
ACACCCAACCCAGAGAACCC+TGG 0.662263 4.2:+21199078 MS.gene035199:CDS
TTATGAACGAGCTGTTCAAG+GGG 0.665698 4.2:+21195171 MS.gene035199:CDS
CCAGCAACGACTCAAGCACA+AGG 0.666384 4.2:+21199492 MS.gene035199:CDS
GAGATCCAGACACAGTTCGT+AGG 0.679080 4.2:+21195251 MS.gene035199:CDS
TGGGAATGCTTATACTGCTG+GGG 0.686552 4.2:+21193403 MS.gene035199:CDS
GAAGAAATATGCTGACCATG+AGG 0.691106 4.2:+21195114 MS.gene035199:CDS
TAATTCCCATAATATGCCTG+AGG 0.692350 4.2:-21200697 None:intergenic
TGGTGTAAATCATTGCAAGG+CGG 0.722048 4.2:-21195984 None:intergenic
CTTGGGAACAGGATTCGAAG+CGG 0.730245 4.2:-21193288 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.2 gene 21193215 21200726 21193215 ID=MS.gene035199
chr4.2 mRNA 21193215 21200726 21193215 ID=MS.gene035199.t1;Parent=MS.gene035199
chr4.2 exon 21193215 21193659 21193215 ID=MS.gene035199.t1.exon1;Parent=MS.gene035199.t1
chr4.2 CDS 21193215 21193659 21193215 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21194525 21194631 21194525 ID=MS.gene035199.t1.exon2;Parent=MS.gene035199.t1
chr4.2 CDS 21194525 21194631 21194525 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21195043 21195272 21195043 ID=MS.gene035199.t1.exon3;Parent=MS.gene035199.t1
chr4.2 CDS 21195043 21195272 21195043 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21195885 21196052 21195885 ID=MS.gene035199.t1.exon4;Parent=MS.gene035199.t1
chr4.2 CDS 21195885 21196052 21195885 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21196220 21196583 21196220 ID=MS.gene035199.t1.exon5;Parent=MS.gene035199.t1
chr4.2 CDS 21196220 21196583 21196220 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21197078 21197221 21197078 ID=MS.gene035199.t1.exon6;Parent=MS.gene035199.t1
chr4.2 CDS 21197078 21197221 21197078 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21197975 21198127 21197975 ID=MS.gene035199.t1.exon7;Parent=MS.gene035199.t1
chr4.2 CDS 21197975 21198127 21197975 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21198211 21198327 21198211 ID=MS.gene035199.t1.exon8;Parent=MS.gene035199.t1
chr4.2 CDS 21198211 21198327 21198211 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21198764 21198844 21198764 ID=MS.gene035199.t1.exon9;Parent=MS.gene035199.t1
chr4.2 CDS 21198764 21198844 21198764 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21198994 21199149 21198994 ID=MS.gene035199.t1.exon10;Parent=MS.gene035199.t1
chr4.2 CDS 21198994 21199149 21198994 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21199249 21199548 21199249 ID=MS.gene035199.t1.exon11;Parent=MS.gene035199.t1
chr4.2 CDS 21199249 21199548 21199249 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21200056 21200175 21200056 ID=MS.gene035199.t1.exon12;Parent=MS.gene035199.t1
chr4.2 CDS 21200056 21200175 21200056 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
chr4.2 exon 21200631 21200726 21200631 ID=MS.gene035199.t1.exon13;Parent=MS.gene035199.t1
chr4.2 CDS 21200631 21200726 21200631 ID=cds.MS.gene035199.t1;Parent=MS.gene035199.t1
Gene Sequence

>MS.gene035199

ATGGGAGATAGTGAAACAATGGTTGCAGAAGCATCTGCTGCTGAGACGTATCCATCGGATGCTTACAACAACACCGCTTCGAATCCTGTTCCCAAGGTTGGTGCTAATGTTCCTGCCCCAGCCGAGGCTGCAGGAGAAGCTGCTGCTGCTCTGCCCAATTCAGGCTATGCATTTACTTATTCTGGTGATGGGAATGCTTATACTGCTGGGGATCCTAATTCTGTACTACAAGCTCAATTTAGTGCAACAAACGAGTCAAAGCAAGCTGCTGGAGTGCCTAATGAGGATTCCAGTCTGGTTGGAAAGGAAGCTCTGGACTCTGCTATGGTCTCTGCTGACCATTCATCTGTAAATGGAAGTGTTGATACAGTTGGACTTGAAAATGGAAATGCATCAGAGAATGTTGATGGGTCGGCTGATGAGAAACAACTCACAGATGCTTACGGTATGTATATGCCTTCTCTGTCTGTTATGTTAGCATACTCTTCAGGAAAAGTGAAGTTATTTCGTTTTTATTGTTATTGAAGTTTTGTTAATAAAAAAATCGGAGTTCAATGTTAAGGGTATGTATAGAGCTTGCAAGTAATATAATTAACCAATGATGGAGTCAAGGACTTAAGATTCAAGTGATTTATTATTAACCAGCAAATAGTTCATTTAGGCCTTGTAGTTCATGTAAGCTTGTAAGTAATACAATTGCATATCAATGTTGAGAACTAATGAATTTTCACCAATATTCATGAATGGAAATAAGAAAATTTTAATCGCTCATAGTAACAATAGTGTTGTCAATTGTGGATAACGGATAAAACAGTAGCGGTTTGTTAATATTCCGCTACGCTATAGCGGTATAGTGGTTGCTGTTTAGCAACACTGAGTAAGAAGCTTTATTTTTTCATGCATTGGAGGTGGCGTTTAAGGCTACTTCCATTTGTTTTTGTCTCAAGTGACTTTGAAATTTTTCCAGGCCTGGTTTGAATTGGATCTTGACCATATTGATATAATAACTAGGTTTATTAGGTGTATAAATACGTTCATACATTTACATTTTTTTTCCATTTTATTGGTTCACCAGTGCAACAAGATATCATGACTTTGGTTTGTAATTTGATGATCACATATTCTAAGCCACTTCTGCGATTTTTGGATGTCTTCTATTTTACTGAGTTGGCTGATTCAGGCCTAAACATACACGTCTTATCTCCATACGTCTTGTTTTTAATATTATCTTGTTATTAATTATTCACAAGCATATTGCTGTTCAACCAAATTATGGTTTTGCTGCTAATCATTTATCTCACGTTTAACAGCTGCGTTGTCTGCTGAAGAAGATCGATTGTGGAATATTGTAACAGCTAATTCTTCTGATTTTACTGCTTGGACTTCTTTGATTGACGAGACAGAGAAGGTGGCAGAGGTATGATATGTTGACCCAATTTTTAAGACGTGAATTACATGTCGAGGAATCAAAATAAATGACTGATCTCGATGTTTTCATATTAGTTTGATATATGACATGCATGCATTACTACGTGTTTAATGAGATGGATAATTTATATTGTTAAATACTTAAACCTGCATCTGTTTAACGGGGGTGTCTTGATTAAGAATGTCTATAATGTACACACAAGTTGTGGGTGATTTCTCTGGTTCTGTCTGTCGGTATATTATTTTTTAACCTGCTAGTATATTATTTTTTCCTTTTTTGTCCCTTATTACGAGATACTTGTAGTTATAGGTTTAGTGGCAAACTAATGTGATGTATTTGATTTTATTATTGATTTATTTTATGACTCATGGTTCTCATATAATGATTTTTTTATTGAAGAACAATATTTTGAAGATGCGTAGAGTCTATGATGCTTTCCTAGCAGAATTCCCTTTGTGTTATGGGTATTGGAAGAAATATGCTGACCATGAGGCGCGCCTTGGCTCTGCTGATAAAGTTGTTGAAGTTTATGAACGAGCTGTTCAAGGGGTTACATATTCAGTGGACATGTGGTTGCATTATTGCATATTCGCTATAAGCACCTATGGAGATCCAGACACAGTTCGTAGGTAATAGAGGCTTATTTACATGTTACCTTATTATTTTCTATGATCGGATTTTCTATTTACTAGGCTCAGCTGTTTCTTGCTTGCTTTCTAACCTGATTTCAGAGACCGTGCAACAGTACCATCTCCTTTTTATGGCGGAAAAGCTTTGATGGGATTGCATGTCAGAATGTTTTCCATTCTGATGTTTAAATCCTTGGTTTTGATTCCACTATGTATAGTAGTTGAAATTTTGGGTCCATTTTCCCCTATAAACTTTTCAATTTGATTTCAAACTTCATAGTTGTGTGGAAGGGTTCTATTTTCCCCCCCTTTCCTGTTGAAATGGTTAGCTGTTTTTATCTTTGACACTATGAGGTGTATCAATTTGCAAATTGCTGCTGGGAAGATCTCGTCTGGTTAAGGGCTAGATGGCCTCTATGACTGCTCCATTTCTCGGACATTGTATGATTTTTTGCAGGAAGGTAAATGCAGCGTTTGGATGTCTTATATGATGTTACGAGTGATTTGTTTGTTACCTTAGAACACTAGTGCTGCTGTTTCCCATACATGGATGTGCATCAGTAAATGGATGCTTTAAGTTACAGATTAATCAAGACTGCTTGCTTATTTCAGGCTTTTTGAACGAGGATTAGCTTATGTTGGAACAGATTATCTTTCGTTTCCTCTTTGGGATAAATACATTGAATATGAGTACATGCAGCAAGACTGGGCCCGCCTTGCAATGATTTACACCAGAATATTAGAGAATCCAAATCAGCAATTGGATCGATATTTCAACAGGTAATTACTTGTTTTAATTTAATATTATCATTCCACTTCATTTGTTTCCAAAAAAGAAACACAATTACTATTTTTTGTTTTATATTTCTACATAATATCCCCTTCTACTATCTCCTCTCTATTTTCCCCCGAACTAAATTACTTCAACGCTGTAAATGGTGTTGCAGCTTCAAGGAGTTGGCTAGTAATCGACCTCTTTCAGAATTACGGACAGCTGATGAAGCTGCTGCAGTGGCAGGTGTTGTTTCAGAGGGTACTGACCAAGGTGTTGAAGGAGAGGTTCATCCTGATGGTGCAGATAATTCTCCTAAACCTGCAAGTGCTGTATTAACAGAAGCAGAAGAGTTGGAGAAGTATATTGCCATTAGAGAAGAGATGTATAAGAAAGCTAAAGAGTTCGATTCTAAGATCATTGGTTTTGAAACAACTATCAGGAGGCCCTACTTTCACGTACGACCTCTTAATATTGGGGAGCTTGAAAACTGGCATAATTATCTGGATTTTATAGAAAGAGAAGGCGACTTGAGCAAGGTAACCAAACTGCTTTCTTATAAGTGCACATTCTGTTCTAGTTTTTCCTGTTATAGATTGACTTACAGTAAGTAATATCTGCTGTATCATATGTCTTATAAGTGCACATCCTATTCTGTTGGATGAAGAAACTTTTTAATCGGGAATTGAACTGAGACTAAAGCATGGGCCTACAAATTGGTTTCTTGAGGCTAAGTACTTCAAACCCTAATTTGTCAGTGGACATTCTAGGTTTGTCATGTGTTTGCAAAAGTTTATTTTTAAAAGTGTGAATGTTATGAATGCAGGTATCTTCTAATCTTCATAATCACCTGTCTCAGGCAAGGAGGGTCCCTCTTTTCATATACGAGGGGCCTCTCTGTCCCGCCACGGAGTATTTCTAATGCTTTGAATTTTCTTTTTATGCTTCTCTTTCTTGATTTTTTTGCTTGCATTTACATTGTTGGACAATTTTTATGATGTGTTCTTTTCTTGTTTTGTTGTTTGTGGATCAGATTGTCAAGTTGTATGAGAGATGTGTCATTGCCTGTGCCAATTATCCCGAGTACTGGATACGCTATGTTTTGTGTATGGAAGCTAGGGAAAGTATGGATCTTGCAAATAATGTTCTCGCTCGGGCGAGCCAAGTTTTTGTCAAGGTAATTTCTTCCAAATGTCAAAGCTGTAGTTTTGGCAGCCATTTGCTGCTTTTTAGTGTTGTATTCATCTGTCCTGTTTTTAAGCTAAGTGAATTGGCAAGAAACACACATATATAATGGACTACTAGCCATAGGATAATAGTTCATGAAACTTGATATAGTGTGTCAATACACCATAGGTTGGTTTTTTGTTTACCTGATTGTAAATGGTGAAGGGGCATCTTATATTTTTGAGCTTTATGTTTATATGATTTCTTTAGGGTAAATGAAAGTTCAATTGTCTTTTATTTCACCATCAGGGCCTTCGTTTAGCCGAGAATAGAAATCTCATGTACACCGCGTTGCCAATGACCCATTTCGTATAAGTGAAGGCGTATAAATGATTTTTTTTTTTTTATATCGCCTGTCTTACACAAGACATATGATCCCTTTGAGGTTGTGACTAGCTAGTGATTCAATTACTCATTTCCTTTTCATGGAGGTATTGGTATTATGTCGTAGATCAGTTATCCTGAAAGCCTAAACTGCCAGGTCACATATACGAAGAGTATTTTCATATCACTGGCAAGCTTTTTGTTGTGGCCCATCAGTGAATGGTGTTTATTATATGTGCAATTTAGTCTTGATTTATAAAAAGCAATGTTCATGTATACTTTGTTTATAAGCATAATTAGCTCAATTGCATTTCTTGTGTGAGAGGCGTTTCCCTTTGTTTTGTTGCTGCTTTATCATTTTTTGCTTGTCATAATGCAGAGACAACCCGAGATACATCTTTTTTGCGCTCGGTTCAAGGAGCAGGCTGGAGATATTGTTGGCGCTAGAGCTGCGTATCAACTTGTTCACACTGAAATTTCTCCAGGTCTTCTTGAAGCAATAATCAGGCATGCCAATATGGAACATCGATTGGTATGCTGACTTACAAAAGCGTTAATAAACCGTGACTCAATTTTTGCTTAAATTTACAGCTTTTCCATTCCATGATATTGCAGGGGAAGTTGGAGGATGCCTTCTCTTTGTATGAACAGGCCATTGCCATTGAGAAAGGAAAAGAACATTCACAAACACTGCCAATGTTGTTTGCTCAGTATTCTCGATTTGTTTATTTGGTAGGTTTGGCAGCATATTTGTATTCTTCGCTTGTTATTCAGTCTTGTTTTGTTTCAGACATTTCCGCATTTGGCTATAGAACATGAAAAAGGGACAGTTCTCTTAATTAACATTAGCTACTGTTTTTGTTGACCTGTCTGGATCTGGCCAAATGATGATTATTTCTGCGAGCCTCTCATTTATCCCAATAAATAGAGATGATTCTGGCTTTATATTATTTAACAATTTTTATATTATTTAACAATTTTCTAGCTTTATGCTTCAGTTGCTTTACTCGTTTGTGTGAAAATTGTCTAGTCTTGTTTTTGAAATATTCTTTCTCATTCTGTCATTAGGCTTCTGGGAATTATAGTTTTATGCTTCAGTTGCTATGCTTGTTTGTGTGAAAGTTGTCTTGTCTTGTTTTTGAAATATTCTTTCTTATTCTGTCATTAGGCTTCTGGGAATTCCGAAAAGGCTAGAGAGATTTTAGTTGGGGGACTTGAGAATGCTTCACTGTCAAAGCCACTACTTGAGGTTTGTCTCAACTTTCACTTTCTATTGATACTTAACTAATATTATGCACTTGGCAATTTTTCTCTTCGTCTTTCCAAATGTTTTTCATATCTAACGAGATTATTCAACTTCATCTCTAACGAGAGTATTTAACTTCATCTCTTACACAGGGTTTATTGCATTTTGAGGCTATTCAACCCCAGCCAAAGCGAGTTGACATTGACTTTCTAGAGTCACTGGTTGTAAAATTCATAACACCCAACCCAGAGAACCCTGGTGTAGCAAGTGCAACAGAGCGCGAGGAGCTTTCTAACATTTTTCTGGAGGTGATTTCTTATGAGGAGGATGTTCAATATTATATTTTATTAGTTTCCTTTAGTTAGCAGTATTATTAAGTTCTTACAAATTTTCTGTTGTTTTTTCAGTTTTTAAATCTCTTTGGAGATGTTCAATCGATCAAAAGGGTTGAGGATAGACATGCCAAACTGTTCTTGCCAAACAGGGGCTTGTCGGAGTTGAAAAAACGTCATGCAGAGGATTTCTTAGCCTCAGATAAAACAAAAGTGTCAAGAGCTTATTCTGCTCAATCACCTGCCCAATCTGTAGTGGGTGCATATCCAAATGGACCTAACCAATGGCCTAACTATGGAGTACAGCCTCAAACTTGGCCAGCAACGACTCAAGCACAAGGACAACAATGGCCTGCTGGCTACACCCAGCAGCAGGTAAATTGGTTTTTGCCCTCAACTATTTTTCTTGTGTGTTGCTTCCCTAGTTCAAATCCCAGTGTTGCATGGTGTTTGTCTATGACGTGGGCGAAATTGTTTTATAAACTAGTGAGTCTTTCTTGCCGGGTTGTGCAATATAAACTTTTTTTTGTTTTTGGAAATAGTGGTGTACACAGGTGTGTCATAACCTTAATCCATGAAGCATTATAATTTGAAAACACCATATTAGCCTTGTTGAAATGGAATTTGCGCCATATCTCATTGTAATGAGTTGATTACCCTTTTAGTTTATATTTGCCTTGAACCCATAAAGACATCTTCATGCATTTTTATCTACAGAAAATCATGTCATAAATGCTCTCCATTCATGCTATGCCCGACAGTTTCATGTTTTCTAGAGTTAAACATTTGTCGCACTTGTAGTGTATGTTTGACACTGTGTTTGTGCATTCTAGAATGGTGAGGTATTCCTGTTCTGAAATTATCCATTCTGATTGAATACAGGCTGCCGCTTCCTATGGGGCTTATGCTGGATATGGAGGCAACTATGCTAACCCACAATTGCCTGCATCAGTTCCACAAAGCACTGCTTATGGGGCCTATCCTCCTGCATATCCTGCACAGGTGAAAAGCTGTTTCAGCCCTTATGATTTTTGCAGATTATTGTTTGCCTGATATGTTTCAGCTCTATAGTTCTTTCTTGCGAGTGTTAATTACAATTTTGCTCTGTAGAACAAGAGAAAGCAAAAAAAGTAAGGGGAATCATGTGATGGTGTTTGTGTGTGTGTATAGCAAGCAAAAAGAATGTAAAATATTATCGAACATACCATTGTTTTATAAGGCCTTTTGGCTTTTTTTTTTTTTTTTTTTTTTGAGGGAACACGTGCCCAGCGTATCAGGATTTTCATTGAGTTGAGTTTCACCAGTTTAATAATTGCACTAATAGTACATAAGTTTGGTTTCATTTCTCAGTTGTATTTGACTTCTCTGACCTTTTGTTACTCTTTCCATTTCTATATTTCTGCTAAACCTGAGTACTGCGAATTAGATTAGTGTTGATTATTTTTTCTGAATTTCAGCCGGCACTGCCTCAACAAAATTATGCACAGCCTGTTGCAGCCCCGACACAGCAACCTGCAGCCGTTCCTCAGGCATATTATGGGAATTACTACTGA
Protein sequence

>MS.gene035199.t1

MGDSETMVAEASAAETYPSDAYNNTASNPVPKVGANVPAPAEAAGEAAAALPNSGYAFTYSGDGNAYTAGDPNSVLQAQFSATNESKQAAGVPNEDSSLVGKEALDSAMVSADHSSVNGSVDTVGLENGNASENVDGSADEKQLTDAYAALSAEEDRLWNIVTANSSDFTAWTSLIDETEKVAENNILKMRRVYDAFLAEFPLCYGYWKKYADHEARLGSADKVVEVYERAVQGVTYSVDMWLHYCIFAISTYGDPDTVRRLFERGLAYVGTDYLSFPLWDKYIEYEYMQQDWARLAMIYTRILENPNQQLDRYFNSFKELASNRPLSELRTADEAAAVAGVVSEGTDQGVEGEVHPDGADNSPKPASAVLTEAEELEKYIAIREEMYKKAKEFDSKIIGFETTIRRPYFHVRPLNIGELENWHNYLDFIEREGDLSKIVKLYERCVIACANYPEYWIRYVLCMEARESMDLANNVLARASQVFVKRQPEIHLFCARFKEQAGDIVGARAAYQLVHTEISPGLLEAIIRHANMEHRLGKLEDAFSLYEQAIAIEKGKEHSQTLPMLFAQYSRFVYLASGNSEKAREILVGGLENASLSKPLLEGLLHFEAIQPQPKRVDIDFLESLVVKFITPNPENPGVASATEREELSNIFLEFLNLFGDVQSIKRVEDRHAKLFLPNRGLSELKKRHAEDFLASDKTKVSRAYSAQSPAQSVVGAYPNGPNQWPNYGVQPQTWPATTQAQGQQWPAGYTQQQAAASYGAYAGYGGNYANPQLPASVPQSTAYGAYPPAYPAQPALPQQNYAQPVAAPTQQPAAVPQAYYGNYY