Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035366.t1 | KEH32297.1 | 99.5 | 188 | 1 | 0 | 1 | 188 | 1 | 188 | 1.00E-98 | 369.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035366.t1 | Q93ZE5 | 76.0 | 183 | 44 | 0 | 5 | 187 | 4 | 186 | 1.8e-75 | 283.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035366.t1 | A0A072URI4 | 99.5 | 188 | 1 | 0 | 1 | 188 | 1 | 188 | 7.5e-99 | 369.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene041603 | MS.gene035366 | PPI |
| MS.gene035366 | MS.gene041601 | PPI |
| MS.gene071503 | MS.gene035366 | PPI |
| MS.gene035366 | MS.gene45012 | PPI |
| MS.gene035366 | MS.gene035368 | PPI |
| MS.gene035366 | MS.gene041603 | PPI |
| MS.gene071505 | MS.gene035366 | PPI |
| MS.gene035366 | MS.gene066362 | PPI |
| MS.gene035366 | MS.gene77824 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035366.t1 | MTR_4g120230 | 99.468 | 188 | 1 | 0 | 1 | 188 | 1 | 188 | 1.59e-137 | 385 |
| MS.gene035366.t1 | MTR_4g120230 | 99.465 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 5.75e-136 | 382 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene035366.t1 | AT4G15940 | 75.410 | 183 | 44 | 1 | 5 | 187 | 4 | 185 | 2.68e-99 | 289 |
| MS.gene035366.t1 | AT3G16700 | 73.770 | 183 | 48 | 0 | 5 | 187 | 4 | 186 | 1.09e-96 | 283 |
| MS.gene035366.t1 | AT3G16700 | 72.414 | 145 | 40 | 0 | 5 | 149 | 4 | 148 | 4.90e-73 | 221 |
| MS.gene035366.t1 | AT3G16700 | 74.286 | 105 | 27 | 0 | 5 | 109 | 4 | 108 | 1.79e-51 | 164 |
| MS.gene035366.t1 | AT3G16700 | 74.286 | 105 | 27 | 0 | 5 | 109 | 4 | 108 | 1.79e-51 | 164 |
Find 0 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTATTATCTTTGTATATC+AGG | + | 32568:13779-13798 | MS.gene035366:intron | 15.0% |
| !!! | AATCAAATTTGAAATTTGAA+AGG | - | 32568:13743-13762 | None:intergenic | 15.0% |
| !! | CTTATGAAGCTAATTAAAAA+TGG | - | 32568:14933-14952 | None:intergenic | 20.0% |
| !! | TGAATTGAATTGAATTGATT+TGG | - | 32568:13480-13499 | None:intergenic | 20.0% |
| !! | TTAGCAATGTGTTATATTTA+AGG | + | 32568:13944-13963 | MS.gene035366:intron | 20.0% |
| !!! | ATTTTAGATGATGAATGTTT+GGG | + | 32568:15037-15056 | MS.gene035366:intron | 20.0% |
| !!! | GAATTGAATTGAATTGATTT+GGG | - | 32568:13479-13498 | None:intergenic | 20.0% |
| !!! | TAATTGATTCACTAATTTTC+AGG | + | 32568:13540-13559 | MS.gene035366:intron | 20.0% |
| !!! | TATTTTAGATGATGAATGTT+TGG | + | 32568:15036-15055 | MS.gene035366:intron | 20.0% |
| ! | ACATATATTGCTAAGTAGTA+TGG | - | 32568:14426-14445 | None:intergenic | 25.0% |
| ! | CAATATATGTCTATCAACAT+AGG | - | 32568:15128-15147 | None:intergenic | 25.0% |
| ! | CTCATATCAATAACATCTAT+GGG | - | 32568:14632-14651 | None:intergenic | 25.0% |
| ! | TAAACTAATTGCATAGAGAT+GGG | + | 32568:14838-14857 | MS.gene035366:intron | 25.0% |
| ! | TCATATCAATAACATCTATG+GGG | - | 32568:14631-14650 | None:intergenic | 25.0% |
| ! | TCTCATATCAATAACATCTA+TGG | - | 32568:14633-14652 | None:intergenic | 25.0% |
| ! | TTGTTGAAAAAGACACTAAT+AGG | - | 32568:14510-14529 | None:intergenic | 25.0% |
| ! | TTTCTTACAATAGGACTAAT+TGG | - | 32568:14077-14096 | None:intergenic | 25.0% |
| AAAAGACACTAATAGGAACT+CGG | - | 32568:14503-14522 | None:intergenic | 30.0% | |
| AACTACAATACTATATGCTG+TGG | - | 32568:14557-14576 | None:intergenic | 30.0% | |
| AAGTTTGAATATCATGTCCT+TGG | - | 32568:14910-14929 | None:intergenic | 30.0% | |
| ACATCGTCTTACTTGAAAAA+TGG | + | 32568:13586-13605 | MS.gene035366:CDS | 30.0% | |
| ACTACAATACTATATGCTGT+GGG | - | 32568:14556-14575 | None:intergenic | 30.0% | |
| AGCATACACTTTCTTACAAT+AGG | - | 32568:14086-14105 | None:intergenic | 30.0% | |
| CAAACATATCTCGCAAATTT+AGG | - | 32568:14318-14337 | None:intergenic | 30.0% | |
| CAGATTAGGAAATTTGAGAA+AGG | - | 32568:13505-13524 | None:intergenic | 30.0% | |
| CATATCAATAACATCTATGG+GGG | - | 32568:14630-14649 | None:intergenic | 30.0% | |
| CTAAACTAATTGCATAGAGA+TGG | + | 32568:14837-14856 | MS.gene035366:intron | 30.0% | |
| GAATCAATTACTACAGTACA+CGG | - | 32568:13530-13549 | None:intergenic | 30.0% | |
| TCTTAAAAGCTAGCTATCAA+GGG | + | 32568:14363-14382 | MS.gene035366:intron | 30.0% | |
| TCTTACATGACATTGTTTGA+AGG | + | 32568:14954-14973 | MS.gene035366:CDS | 30.0% | |
| TTGTATATCAGGTTATGCTT+TGG | + | 32568:13790-13809 | MS.gene035366:intron | 30.0% | |
| ! | CATCTCTATGCAATTAGTTT+AGG | - | 32568:14839-14858 | None:intergenic | 30.0% |
| ! | TGATCTAGAATTATGGCTAA+AGG | + | 32568:14756-14775 | MS.gene035366:CDS | 30.0% |
| !!! | AGTTTTTTTGCACTTCTTCT+TGG | + | 32568:14783-14802 | MS.gene035366:intron | 30.0% |
| !!! | TTGATAGCTAGCTTTTAAGA+GGG | - | 32568:14363-14382 | None:intergenic | 30.0% |
| !!! | TTTTTCAAGTAAGACGATGT+TGG | - | 32568:13586-13605 | None:intergenic | 30.0% |
| !!! | TTTTTTTCCAAGAACGGTTT+CGG | - | 32568:15233-15252 | None:intergenic | 30.0% |
| AAAGACACTAATAGGAACTC+GGG | - | 32568:14502-14521 | None:intergenic | 35.0% | |
| AACAATTACACTTGAACCAC+TGG | + | 32568:13875-13894 | MS.gene035366:intron | 35.0% | |
| AATTCCATATGACATGCACA+CGG | - | 32568:14275-14294 | None:intergenic | 35.0% | |
| AATTCTAGATCATCAGGGTT+TGG | - | 32568:14748-14767 | None:intergenic | 35.0% | |
| ACAATTACACTTGAACCACT+GGG | + | 32568:13876-13895 | MS.gene035366:intron | 35.0% | |
| AGCCATAATTCTAGATCATC+AGG | - | 32568:14754-14773 | None:intergenic | 35.0% | |
| CTCTTAAAAGCTAGCTATCA+AGG | + | 32568:14362-14381 | MS.gene035366:intron | 35.0% | |
| CTTAAAAGCTAGCTATCAAG+GGG | + | 32568:14364-14383 | MS.gene035366:intron | 35.0% | |
| GAAATGATTCCACTTTGTCT+TGG | + | 32568:14155-14174 | MS.gene035366:intron | 35.0% | |
| GCCATAATTCTAGATCATCA+GGG | - | 32568:14753-14772 | None:intergenic | 35.0% | |
| TCGTCTTACTTGAAAAATGG+CGG | + | 32568:13589-13608 | MS.gene035366:CDS | 35.0% | |
| TGAAACAATCCAAGACAAAG+TGG | - | 32568:14167-14186 | None:intergenic | 35.0% | |
| TGTAGGTAGATGAGGAAATT+CGG | + | 32568:14871-14890 | MS.gene035366:intron | 35.0% | |
| TTAAAAGCTAGCTATCAAGG+GGG | + | 32568:14365-14384 | MS.gene035366:intron | 35.0% | |
| TTGTCAGAAGCTCTTTGATT+TGG | + | 32568:13363-13382 | MS.gene035366:CDS | 35.0% | |
| ! | ACCCTGATGATCTAGAATTA+TGG | + | 32568:14749-14768 | MS.gene035366:CDS | 35.0% |
| ! | TGTCAGAAGCTCTTTGATTT+GGG | + | 32568:13364-13383 | MS.gene035366:CDS | 35.0% |
| !! | GTTGGTTTGAGAAACAATAC+TGG | - | 32568:13568-13587 | None:intergenic | 35.0% |
| !!! | CTTGATAGCTAGCTTTTAAG+AGG | - | 32568:14364-14383 | None:intergenic | 35.0% |
| !!! | TTTTTTCCAAGAACGGTTTC+GGG | - | 32568:15232-15251 | None:intergenic | 35.0% |
| AAGTATACGAGCCTTTGCAA+AGG | + | 32568:15162-15181 | MS.gene035366:CDS | 40.0% | |
| ACCCTCGATAATAAGGTCTT+GGG | + | 32568:15094-15113 | MS.gene035366:CDS | 40.0% | |
| ACCTGCAGACTAACAACAAA+AGG | - | 32568:14015-14034 | None:intergenic | 40.0% | |
| AGAAACATCACGAGCTTTCT+TGG | - | 32568:13680-13699 | None:intergenic | 40.0% | |
| ATCGAGTACCCTCGATAATA+AGG | + | 32568:15087-15106 | MS.gene035366:CDS | 40.0% | |
| ATTCCATATGACATGCACAC+GGG | - | 32568:14274-14293 | None:intergenic | 40.0% | |
| CATACTTATGTACATCCCAG+TGG | - | 32568:13894-13913 | None:intergenic | 40.0% | |
| CCTGCAGACTAACAACAAAA+GGG | - | 32568:14014-14033 | None:intergenic | 40.0% | |
| GAAGGAGATGTCATTCTAAC+TGG | + | 32568:14972-14991 | MS.gene035366:CDS | 40.0% | |
| GCACTTCTTCTTGGATACTT+TGG | + | 32568:14792-14811 | MS.gene035366:intron | 40.0% | |
| GCTAAGTAGTATGGAATGCT+CGG | - | 32568:14417-14436 | None:intergenic | 40.0% | |
| GTATCAAGTATCAAGTCACG+TGG | + | 32568:14452-14471 | MS.gene035366:intron | 40.0% | |
| TAATTGGTGTGAACGTATCC+TGG | - | 32568:14061-14080 | None:intergenic | 40.0% | |
| TACAGTACACGGATCAGATT+AGG | - | 32568:13519-13538 | None:intergenic | 40.0% | |
| TACCCTCGATAATAAGGTCT+TGG | + | 32568:15093-15112 | MS.gene035366:CDS | 40.0% | |
| TATACCAGCAACGTAATCCA+TGG | - | 32568:13713-13732 | None:intergenic | 40.0% | |
| TATCAAGTCACGTGGATACT+CGG | + | 32568:14460-14479 | MS.gene035366:intron | 40.0% | |
| TCAACTGAACCAACACTTGA+TGG | - | 32568:15063-15082 | None:intergenic | 40.0% | |
| TGTAGACCAAGTCCTTGTAA+AGG | - | 32568:14238-14257 | None:intergenic | 40.0% | |
| TTACCACAAGCAATGAGTCA+TGG | + | 32568:14603-14622 | MS.gene035366:intron | 40.0% | |
| TTACCTTAGCAGCAGATTGA+AGG | - | 32568:13838-13857 | None:intergenic | 40.0% | |
| ! | AAGAGTGTGTTTGTGACCTT+GGG | - | 32568:13454-13473 | None:intergenic | 40.0% |
| ! | AGATGGGTTTGCTTTGTTGT+AGG | + | 32568:14854-14873 | MS.gene035366:intron | 40.0% |
| ! | TGCTTTGTTGTAGGTAGATG+AGG | + | 32568:14863-14882 | MS.gene035366:intron | 40.0% |
| ! | TTTTTCCAAGAACGGTTTCG+GGG | - | 32568:15231-15250 | None:intergenic | 40.0% |
| !! | CAAGTGTTGGTTCAGTTGAT+TGG | + | 32568:15064-15083 | MS.gene035366:CDS | 40.0% |
| ACCCCAAGACCTTATTATCG+AGG | - | 32568:15099-15118 | None:intergenic | 45.0% | |
| AGACGTTGATCGCCTTTACA+AGG | + | 32568:14223-14242 | MS.gene035366:intron | 45.0% | |
| AGATGAGGAAATTCGGCAGA+AGG | + | 32568:14878-14897 | MS.gene035366:CDS | 45.0% | |
| ATAGGAACTCGGGTATTACG+CGG | - | 32568:14492-14511 | None:intergenic | 45.0% | |
| ATCAAGTCACGTGGATACTC+GGG | + | 32568:14461-14480 | MS.gene035366:intron | 45.0% | |
| ATCAGGTTATGCTTTGGCAC+TGG | + | 32568:13796-13815 | MS.gene035366:intron | 45.0% | |
| ATGTTTGGGCCATCAAGTGT+TGG | + | 32568:15051-15070 | MS.gene035366:CDS | 45.0% | |
| CAAGCCATGATCCTTTGCAA+AGG | - | 32568:15176-15195 | None:intergenic | 45.0% | |
| CCCCAAGACCTTATTATCGA+GGG | - | 32568:15098-15117 | None:intergenic | 45.0% | |
| CCCTCGATAATAAGGTCTTG+GGG | + | 32568:15095-15114 | MS.gene035366:CDS | 45.0% | |
| CTTCCATGGAGTTTGGCAAA+AGG | + | 32568:14035-14054 | MS.gene035366:CDS | 45.0% | |
| GATGAGGAAATTCGGCAGAA+GGG | + | 32568:14879-14898 | MS.gene035366:CDS | 45.0% | |
| GATTTGGGCACGAAGATCAT+CGG | + | 32568:13379-13398 | MS.gene035366:CDS | 45.0% | |
| TGATCGCCTTTACAAGGACT+TGG | + | 32568:14229-14248 | MS.gene035366:intron | 45.0% | |
| TGTGTTTGTGACCTTGGGAA+CGG | - | 32568:13449-13468 | None:intergenic | 45.0% | |
| TTACCCGTGTGCATGTCATA+TGG | + | 32568:14268-14287 | MS.gene035366:intron | 45.0% | |
| ! | GAAGAGTGTGTTTGTGACCT+TGG | - | 32568:13455-13474 | None:intergenic | 45.0% |
| ! | GCCTAGTTCTTTAGCATGAG+CGG | - | 32568:13422-13441 | None:intergenic | 45.0% |
| ! | TGTTTCTGAATCCTCTGCCA+TGG | + | 32568:13693-13712 | MS.gene035366:CDS | 45.0% |
| !!! | CCCTTTTGTTGTTAGTCTGC+AGG | + | 32568:14011-14030 | MS.gene035366:intron | 45.0% |
| AAGAACGGTTTCGGGGAGAA+GGG | - | 32568:15224-15243 | None:intergenic | 50.0% | |
| AGCAACGTAATCCATGGCAG+AGG | - | 32568:13707-13726 | None:intergenic | 50.0% | |
| AGCTTTCTTGGCGATGACGA+CGG | - | 32568:13668-13687 | None:intergenic | 50.0% | |
| ATGGAGTTTGGCAAAAGGCC+AGG | + | 32568:14040-14059 | MS.gene035366:CDS | 50.0% | |
| CGAGCCTTTGCAAAGGATCA+TGG | + | 32568:15169-15188 | MS.gene035366:CDS | 50.0% | |
| GCCGCTCATGCTAAAGAACT+AGG | + | 32568:13418-13437 | MS.gene035366:CDS | 50.0% | |
| GGACCATGACTCATTGCTTG+TGG | - | 32568:14609-14628 | None:intergenic | 50.0% | |
| GGACCTTCAATCTGCTGCTA+AGG | + | 32568:13832-13851 | MS.gene035366:CDS | 50.0% | |
| TGCAGGTCTTCCATGGAGTT+TGG | + | 32568:14028-14047 | MS.gene035366:CDS | 50.0% | |
| TGGAGAGAACTCTCGTTGTG+TGG | - | 32568:13628-13647 | None:intergenic | 50.0% | |
| ! | AGGGTTTGGTACTGCAGTCT+TGG | - | 32568:14734-14753 | None:intergenic | 50.0% |
| ! | GGCACTGGATATGACTGCTA+GGG | + | 32568:13811-13830 | MS.gene035366:CDS | 50.0% |
| ! | GGGTTTGGTACTGCAGTCTT+GGG | - | 32568:14733-14752 | None:intergenic | 50.0% |
| ! | GGTAGAGTATCCACGTGACT+TGG | - | 32568:14396-14415 | None:intergenic | 50.0% |
| ! | GTTAGTCTGCAGGTCTTCCA+TGG | + | 32568:14021-14040 | MS.gene035366:intron | 50.0% |
| ! | TCTGCCATGGATTACGTTGC+TGG | + | 32568:13706-13725 | MS.gene035366:CDS | 50.0% |
| ! | TGGCACTGGATATGACTGCT+AGG | + | 32568:13810-13829 | MS.gene035366:CDS | 50.0% |
| ! | TGGCCTTTTGCCAAACTCCA+TGG | - | 32568:14041-14060 | None:intergenic | 50.0% |
| CAAGAACGGTTTCGGGGAGA+AGG | - | 32568:15225-15244 | None:intergenic | 55.0% | |
| CTTCTCCCCGAAACCGTTCT+TGG | + | 32568:15223-15242 | MS.gene035366:CDS | 55.0% | |
| CTTGTCGCGCTCTTGGAAAG+GGG | + | 32568:15192-15211 | MS.gene035366:CDS | 55.0% | |
| GCTTGTCGCGCTCTTGGAAA+GGG | + | 32568:15191-15210 | MS.gene035366:CDS | 55.0% | |
| ! | ATCATGGCTTGTCGCGCTCT+TGG | + | 32568:15185-15204 | MS.gene035366:CDS | 55.0% |
| AACGGTTTCGGGGAGAAGGG+AGG | - | 32568:15221-15240 | None:intergenic | 60.0% | |
| ACTAGGCAACGCCGTTCCCA+AGG | + | 32568:13435-13454 | MS.gene035366:CDS | 60.0% | |
| CGGCGAGTTCGACTTCGTGA+TGG | - | 32568:13648-13667 | None:intergenic | 60.0% | |
| GGCACGAAGATCATCGGCGT+CGG | + | 32568:13385-13404 | MS.gene035366:CDS | 60.0% | |
| GGCTTGTCGCGCTCTTGGAA+AGG | + | 32568:15190-15209 | MS.gene035366:CDS | 60.0% | |
| GGGGGAAGAACCAAGTCACG+TGG | + | 32568:14383-14402 | MS.gene035366:intron | 60.0% | |
| TCGGCAGAAGGGCTCAACCA+AGG | + | 32568:14890-14909 | MS.gene035366:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32568 | gene | 13346 | 15255 | 13346 | ID=MS.gene035366 |
| 32568 | mRNA | 13346 | 15255 | 13346 | ID=MS.gene035366.t1;Parent=MS.gene035366 |
| 32568 | exon | 13346 | 13456 | 13346 | ID=MS.gene035366.t1.exon1;Parent=MS.gene035366.t1 |
| 32568 | CDS | 13346 | 13456 | 13346 | ID=cds.MS.gene035366.t1;Parent=MS.gene035366.t1 |
| 32568 | exon | 13562 | 13727 | 13562 | ID=MS.gene035366.t1.exon2;Parent=MS.gene035366.t1 |
| 32568 | CDS | 13562 | 13727 | 13562 | ID=cds.MS.gene035366.t1;Parent=MS.gene035366.t1 |
| 32568 | exon | 13801 | 13853 | 13801 | ID=MS.gene035366.t1.exon3;Parent=MS.gene035366.t1 |
| 32568 | CDS | 13801 | 13853 | 13801 | ID=cds.MS.gene035366.t1;Parent=MS.gene035366.t1 |
| 32568 | exon | 14026 | 14088 | 14026 | ID=MS.gene035366.t1.exon4;Parent=MS.gene035366.t1 |
| 32568 | CDS | 14026 | 14088 | 14026 | ID=cds.MS.gene035366.t1;Parent=MS.gene035366.t1 |
| 32568 | exon | 14727 | 14777 | 14727 | ID=MS.gene035366.t1.exon5;Parent=MS.gene035366.t1 |
| 32568 | CDS | 14727 | 14777 | 14727 | ID=cds.MS.gene035366.t1;Parent=MS.gene035366.t1 |
| 32568 | exon | 14876 | 14993 | 14876 | ID=MS.gene035366.t1.exon6;Parent=MS.gene035366.t1 |
| 32568 | CDS | 14876 | 14993 | 14876 | ID=cds.MS.gene035366.t1;Parent=MS.gene035366.t1 |
| 32568 | exon | 15044 | 15255 | 15044 | ID=MS.gene035366.t1.exon7;Parent=MS.gene035366.t1 |
| 32568 | CDS | 15044 | 15255 | 15044 | ID=cds.MS.gene035366.t1;Parent=MS.gene035366.t1 |
| Gene Sequence |
| Protein sequence |
