Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041601.t1 | XP_013458265.1 | 99.5 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 4.90E-98 | 367.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041601.t1 | Q93ZE5 | 76.0 | 183 | 44 | 0 | 5 | 187 | 4 | 186 | 1.7e-75 | 283.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041601.t1 | A0A072URI4 | 99.5 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 3.5e-98 | 367.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene035366 | MS.gene041601 | PPI |
| MS.gene041603 | MS.gene041601 | PPI |
| MS.gene041601 | MS.gene77824 | PPI |
| MS.gene071503 | MS.gene041601 | PPI |
| MS.gene071505 | MS.gene041601 | PPI |
| MS.gene77824 | MS.gene041601 | PPI |
| MS.gene035368 | MS.gene041601 | PPI |
| MS.gene066362 | MS.gene041601 | PPI |
| MS.gene45012 | MS.gene041601 | PPI |
| MS.gene041601 | MS.gene066362 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041601.t1 | MTR_4g120230 | 99.465 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 1.14e-137 | 382 |
| MS.gene041601.t1 | MTR_4g120230 | 99.465 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 4.51e-137 | 382 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene041601.t1 | AT4G15940 | 75.410 | 183 | 44 | 1 | 5 | 187 | 4 | 185 | 1.25e-100 | 290 |
| MS.gene041601.t1 | AT3G16700 | 73.770 | 183 | 48 | 0 | 5 | 187 | 4 | 186 | 4.69e-98 | 283 |
| MS.gene041601.t1 | AT3G16700 | 72.414 | 145 | 40 | 0 | 5 | 149 | 4 | 148 | 2.58e-74 | 221 |
| MS.gene041601.t1 | AT3G16700 | 74.286 | 105 | 27 | 0 | 5 | 109 | 4 | 108 | 1.49e-52 | 164 |
| MS.gene041601.t1 | AT3G16700 | 74.286 | 105 | 27 | 0 | 5 | 109 | 4 | 108 | 1.49e-52 | 164 |
Find 0 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTATTATCTTTGTATATC+AGG | + | 32569:24001-24020 | MS.gene041601:intron | 15.0% |
| !!! | AATCAAATTTGAAATTTGAA+AGG | - | 32569:23965-23984 | MS.gene041601:intergenic | 15.0% |
| !! | CTTATGAAGCTAATTAAAAA+TGG | - | 32569:25133-25152 | MS.gene041601:intergenic | 20.0% |
| !! | TGAATTGAATTGAATTGATT+TGG | - | 32569:23702-23721 | MS.gene041601:intergenic | 20.0% |
| !! | TTAGCAATGTGTTATATTTA+AGG | + | 32569:24164-24183 | MS.gene041601:intron | 20.0% |
| !!! | GAATTGAATTGAATTGATTT+GGG | - | 32569:23701-23720 | MS.gene041601:intergenic | 20.0% |
| !!! | TAATTGATTCACTAATTTTC+AGG | + | 32569:23762-23781 | MS.gene041601:intron | 20.0% |
| ! | CTCATATCAATAACATCTAT+GGG | - | 32569:24832-24851 | MS.gene041601:intergenic | 25.0% |
| ! | TAAACTAATTGCATAGAGAT+GGG | + | 32569:25038-25057 | MS.gene041601:intron | 25.0% |
| ! | TCATATCAATAACATCTATG+GGG | - | 32569:24831-24850 | MS.gene041601:intergenic | 25.0% |
| ! | TCTCATATCAATAACATCTA+TGG | - | 32569:24833-24852 | MS.gene041601:intergenic | 25.0% |
| ! | TTGTTGAAAAAGACACTAAT+AGG | - | 32569:24710-24729 | MS.gene041601:intergenic | 25.0% |
| ! | TTTCTTACAATAGGACTAAT+TGG | - | 32569:24297-24316 | MS.gene041601:intergenic | 25.0% |
| AAAAGACACTAATAGGAACT+CGG | - | 32569:24703-24722 | MS.gene041600:intergenic | 30.0% | |
| AACTACAATACTATATGCTG+TGG | - | 32569:24757-24776 | MS.gene041601:intergenic | 30.0% | |
| AAGTTTGAATATCATGTCCT+TGG | - | 32569:25110-25129 | MS.gene041601:intergenic | 30.0% | |
| ACATCGTCTTACTTGAAAAA+TGG | + | 32569:23808-23827 | MS.gene041601:CDS | 30.0% | |
| ACTACAATACTATATGCTGT+GGG | - | 32569:24756-24775 | MS.gene041601:intergenic | 30.0% | |
| AGCATACACTTTCTTACAAT+AGG | - | 32569:24306-24325 | MS.gene041601:intergenic | 30.0% | |
| CAAACATATCTCGCAAATTT+AGG | - | 32569:24531-24550 | MS.gene041601:intergenic | 30.0% | |
| CAGATTAGGAAATTTGAGAA+AGG | - | 32569:23727-23746 | MS.gene041601:intergenic | 30.0% | |
| CATATCAATAACATCTATGG+GGG | - | 32569:24830-24849 | MS.gene041601:intergenic | 30.0% | |
| CTAAACTAATTGCATAGAGA+TGG | + | 32569:25037-25056 | MS.gene041601:intron | 30.0% | |
| GAATCAATTACTACAGTACA+CGG | - | 32569:23752-23771 | MS.gene041601:intergenic | 30.0% | |
| TCTTAAAAGCTAGCTATCAA+GGG | + | 32569:24578-24597 | MS.gene041601:intron | 30.0% | |
| TCTTACATGACATTGTTTGA+AGG | + | 32569:25154-25173 | MS.gene041601:CDS | 30.0% | |
| TTGTATATCAGGTTATGCTT+TGG | + | 32569:24012-24031 | MS.gene041601:intron | 30.0% | |
| TTGTCAGAAACTCTTTGATT+TGG | + | 32569:23585-23604 | MS.gene041601:CDS | 30.0% | |
| ! | CATCTCTATGCAATTAGTTT+AGG | - | 32569:25039-25058 | MS.gene041601:intergenic | 30.0% |
| ! | TGATCTAGAATTATGGCTAA+AGG | + | 32569:24956-24975 | MS.gene041601:CDS | 30.0% |
| ! | TGTCAGAAACTCTTTGATTT+GGG | + | 32569:23586-23605 | MS.gene041601:CDS | 30.0% |
| !!! | AGTTTTTTTGCACTTCTTCT+TGG | + | 32569:24983-25002 | MS.gene041601:intron | 30.0% |
| !!! | TTTTTCAAGTAAGACGATGT+TGG | - | 32569:23808-23827 | MS.gene041601:intergenic | 30.0% |
| AAAGACACTAATAGGAACTC+GGG | - | 32569:24702-24721 | MS.gene041600:intergenic | 35.0% | |
| AACAATTACACTTGAACCAC+TGG | + | 32569:24095-24114 | MS.gene041601:intron | 35.0% | |
| AATTCCATATGACATGCACA+CGG | - | 32569:24493-24512 | MS.gene041601:intergenic | 35.0% | |
| AATTCTAGATCATCAGGGTT+TGG | - | 32569:24948-24967 | MS.gene041601:intergenic | 35.0% | |
| ACAATTACACTTGAACCACT+GGG | + | 32569:24096-24115 | MS.gene041601:intron | 35.0% | |
| ACTTGATGTGCTAAGTAGTA+TGG | - | 32569:24641-24660 | MS.gene041601:intergenic | 35.0% | |
| AGCCATAATTCTAGATCATC+AGG | - | 32569:24954-24973 | MS.gene041601:intergenic | 35.0% | |
| CTCTTAAAAGCTAGCTATCA+AGG | + | 32569:24577-24596 | MS.gene041601:intron | 35.0% | |
| CTTAAAAGCTAGCTATCAAG+GGG | + | 32569:24579-24598 | MS.gene041601:intron | 35.0% | |
| GAAATGATTCCACTTTGTCT+TGG | + | 32569:24373-24392 | MS.gene041601:intron | 35.0% | |
| GCCATAATTCTAGATCATCA+GGG | - | 32569:24953-24972 | MS.gene041601:intergenic | 35.0% | |
| TCGTCTTACTTGAAAAATGG+CGG | + | 32569:23811-23830 | MS.gene041601:CDS | 35.0% | |
| TGAAACAATCCAAGACAAAG+TGG | - | 32569:24385-24404 | MS.gene041601:intergenic | 35.0% | |
| TGTAGGTAGATGAGGAAATT+CGG | + | 32569:25071-25090 | MS.gene041601:intron | 35.0% | |
| TTAAAAGCTAGCTATCAAGG+GGG | + | 32569:24580-24599 | MS.gene041601:intron | 35.0% | |
| ! | ACCCTGATGATCTAGAATTA+TGG | + | 32569:24949-24968 | MS.gene041601:CDS | 35.0% |
| !!! | GATAGCTAGCTTTTAAGAGA+GGG | - | 32569:24576-24595 | MS.gene041601:intergenic | 35.0% |
| !!! | TGATAGCTAGCTTTTAAGAG+AGG | - | 32569:24577-24596 | MS.gene041601:intergenic | 35.0% |
| ACATCAAGTATCAAGTCACG+TGG | + | 32569:24652-24671 | MS.gene041601:intron | 40.0% | |
| ACCTGCAGACTAACAACAAA+AGG | - | 32569:24235-24254 | MS.gene041601:intergenic | 40.0% | |
| AGAAACATCACGAGCTTTCT+TGG | - | 32569:23902-23921 | MS.gene041601:intergenic | 40.0% | |
| ATTCCATATGACATGCACAC+GGG | - | 32569:24492-24511 | MS.gene041601:intergenic | 40.0% | |
| CATACTTATGTACATCCCAG+TGG | - | 32569:24114-24133 | MS.gene041601:intergenic | 40.0% | |
| CCTGCAGACTAACAACAAAA+GGG | - | 32569:24234-24253 | MS.gene041601:intergenic | 40.0% | |
| GCACTTCTTCTTGGATACTT+TGG | + | 32569:24992-25011 | MS.gene041601:intron | 40.0% | |
| GCTAAGTAGTATGGAATGCT+CGG | - | 32569:24632-24651 | MS.gene041601:intergenic | 40.0% | |
| TAATTGGTGTGAACGTATCC+TGG | - | 32569:24281-24300 | MS.gene041601:intergenic | 40.0% | |
| TACAGTACACGGATCAGATT+AGG | - | 32569:23741-23760 | MS.gene041601:intergenic | 40.0% | |
| TATACCAGCAACGTAATCCA+TGG | - | 32569:23935-23954 | MS.gene041601:intergenic | 40.0% | |
| TATCAAGTCACGTGGATACT+CGG | + | 32569:24660-24679 | MS.gene041601:intron | 40.0% | |
| TGTAGACCAAGTGCTTGTAA+AGG | - | 32569:24456-24475 | MS.gene041601:intergenic | 40.0% | |
| TTACCACAAGCAATGAGTCA+TGG | + | 32569:24803-24822 | MS.gene041601:intron | 40.0% | |
| TTACCTTAGCAGCAGATTGA+AGG | - | 32569:24060-24079 | MS.gene041601:intergenic | 40.0% | |
| ! | AAGAGTGTGTTTGTGACCTT+GGG | - | 32569:23676-23695 | MS.gene041601:intergenic | 40.0% |
| ! | AGATGGGTTTGCTTTGTTGT+AGG | + | 32569:25054-25073 | MS.gene041601:intron | 40.0% |
| ! | TGCTTTGTTGTAGGTAGATG+AGG | + | 32569:25063-25082 | MS.gene041601:intron | 40.0% |
| !! | GTTGGTTTGAGAAACAACAC+TGG | - | 32569:23790-23809 | MS.gene041601:intergenic | 40.0% |
| AGATGAGGAAATTCGGCAGA+AGG | + | 32569:25078-25097 | MS.gene041601:CDS | 45.0% | |
| ATAGGAACTCGGGTATTACG+CGG | - | 32569:24692-24711 | MS.gene041601:intergenic | 45.0% | |
| ATCAAGTCACGTGGATACTC+GGG | + | 32569:24661-24680 | MS.gene041601:intron | 45.0% | |
| ATCAGGTTATGCTTTGGCAC+TGG | + | 32569:24018-24037 | MS.gene041601:intron | 45.0% | |
| CTTCCATGGAGTTTGGCAAA+AGG | + | 32569:24255-24274 | MS.gene041601:CDS | 45.0% | |
| GATGAGGAAATTCGGCAGAA+GGG | + | 32569:25079-25098 | MS.gene041601:CDS | 45.0% | |
| GATTTGGGCACGAAGATCAT+CGG | + | 32569:23601-23620 | MS.gene041601:CDS | 45.0% | |
| GCTGCTCATGCTAAAGAACT+AGG | + | 32569:23640-23659 | MS.gene041601:CDS | 45.0% | |
| TGATCGCCTTTACAAGCACT+TGG | + | 32569:24447-24466 | MS.gene041601:intron | 45.0% | |
| TGTGTTTGTGACCTTGGGAA+CGG | - | 32569:23671-23690 | MS.gene041601:intergenic | 45.0% | |
| TTACCCGTGTGCATGTCATA+TGG | + | 32569:24486-24505 | MS.gene041601:intron | 45.0% | |
| ! | GAAGAGTGTGTTTGTGACCT+TGG | - | 32569:23677-23696 | MS.gene041601:intergenic | 45.0% |
| ! | TGTTTCTGAATCCTCTGCCA+TGG | + | 32569:23915-23934 | MS.gene041601:CDS | 45.0% |
| !!! | CCCTTTTGTTGTTAGTCTGC+AGG | + | 32569:24231-24250 | MS.gene041601:intron | 45.0% |
| AGCAACGTAATCCATGGCAG+AGG | - | 32569:23929-23948 | MS.gene041601:intergenic | 50.0% | |
| AGCTTTCTTGGCGATGACGA+CGG | - | 32569:23890-23909 | MS.gene041601:intergenic | 50.0% | |
| ATGGAGTTTGGCAAAAGGCC+AGG | + | 32569:24260-24279 | MS.gene041601:CDS | 50.0% | |
| GGACCATGACTCATTGCTTG+TGG | - | 32569:24809-24828 | MS.gene041601:intergenic | 50.0% | |
| GGACCTTCAATCTGCTGCTA+AGG | + | 32569:24054-24073 | MS.gene041601:CDS | 50.0% | |
| TGCAGGTCTTCCATGGAGTT+TGG | + | 32569:24248-24267 | MS.gene041601:CDS | 50.0% | |
| TGGAGAGAACTCTCGTTGTG+TGG | - | 32569:23850-23869 | MS.gene041601:intergenic | 50.0% | |
| ! | AGGGTTTGGTACTGCAGTCT+TGG | - | 32569:24934-24953 | MS.gene041601:intergenic | 50.0% |
| ! | GGCACTGGATATGACTGCTA+GGG | + | 32569:24033-24052 | MS.gene041601:CDS | 50.0% |
| ! | GGGTTTGGTACTGCAGTCTT+GGG | - | 32569:24933-24952 | MS.gene041601:intergenic | 50.0% |
| ! | GGTAGAGTATCCACGTGACT+TGG | - | 32569:24611-24630 | MS.gene041601:intergenic | 50.0% |
| ! | GTTAGTCTGCAGGTCTTCCA+TGG | + | 32569:24241-24260 | MS.gene041601:intron | 50.0% |
| ! | TCTGCCATGGATTACGTTGC+TGG | + | 32569:23928-23947 | MS.gene041601:CDS | 50.0% |
| ! | TGGCACTGGATATGACTGCT+AGG | + | 32569:24032-24051 | MS.gene041601:CDS | 50.0% |
| ! | TGGCCTTTTGCCAAACTCCA+TGG | - | 32569:24261-24280 | MS.gene041601:intergenic | 50.0% |
| ACTAGGCAACGCCGTTCCCA+AGG | + | 32569:23657-23676 | MS.gene041601:CDS | 60.0% | |
| CGGCGAGTTCGACTTCGTGA+TGG | - | 32569:23870-23889 | MS.gene041601:intergenic | 60.0% | |
| GGCACGAAGATCATCGGCGT+CGG | + | 32569:23607-23626 | MS.gene041601:CDS | 60.0% | |
| GGGGGAAGAACCAAGTCACG+TGG | + | 32569:24598-24617 | MS.gene041601:intron | 60.0% | |
| TCGGCAGAAGGGCTCAACCA+AGG | + | 32569:25090-25109 | MS.gene041601:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32569 | gene | 23568 | 25193 | 23568 | ID=MS.gene041601 |
| 32569 | mRNA | 23568 | 25193 | 23568 | ID=MS.gene041601.t1;Parent=MS.gene041601 |
| 32569 | exon | 23568 | 23678 | 23568 | ID=MS.gene041601.t1.exon1;Parent=MS.gene041601.t1 |
| 32569 | CDS | 23568 | 23678 | 23568 | ID=cds.MS.gene041601.t1;Parent=MS.gene041601.t1 |
| 32569 | exon | 23784 | 23949 | 23784 | ID=MS.gene041601.t1.exon2;Parent=MS.gene041601.t1 |
| 32569 | CDS | 23784 | 23949 | 23784 | ID=cds.MS.gene041601.t1;Parent=MS.gene041601.t1 |
| 32569 | exon | 24023 | 24075 | 24023 | ID=MS.gene041601.t1.exon3;Parent=MS.gene041601.t1 |
| 32569 | CDS | 24023 | 24075 | 24023 | ID=cds.MS.gene041601.t1;Parent=MS.gene041601.t1 |
| 32569 | exon | 24246 | 24308 | 24246 | ID=MS.gene041601.t1.exon4;Parent=MS.gene041601.t1 |
| 32569 | CDS | 24246 | 24308 | 24246 | ID=cds.MS.gene041601.t1;Parent=MS.gene041601.t1 |
| 32569 | exon | 24927 | 24977 | 24927 | ID=MS.gene041601.t1.exon5;Parent=MS.gene041601.t1 |
| 32569 | CDS | 24927 | 24977 | 24927 | ID=cds.MS.gene041601.t1;Parent=MS.gene041601.t1 |
| 32569 | exon | 25076 | 25193 | 25076 | ID=MS.gene041601.t1.exon6;Parent=MS.gene041601.t1 |
| 32569 | CDS | 25076 | 25193 | 25076 | ID=cds.MS.gene041601.t1;Parent=MS.gene041601.t1 |
| Gene Sequence |
| Protein sequence |
