AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81406


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81406.t1 MTR_5g094340 94.484 417 22 1 1 416 1 417 0.0 773
MS.gene81406.t1 MTR_3g055960 73.441 433 95 8 1 416 3 432 0.0 600
MS.gene81406.t1 MTR_5g089720 39.234 418 188 11 53 416 32 437 3.61e-85 266
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81406.t1 AT5G55530 55.140 428 157 12 1 416 1 405 3.06e-145 419
MS.gene81406.t1 AT5G55530 55.140 428 157 12 1 416 1 405 3.06e-145 419
MS.gene81406.t1 AT5G55530 55.140 428 157 12 1 416 1 405 3.06e-145 419
MS.gene81406.t1 AT1G50570 51.522 427 157 12 1 416 1 388 2.91e-125 368
MS.gene81406.t1 AT1G50570 51.522 427 157 12 1 416 1 388 2.91e-125 368
MS.gene81406.t1 AT5G12300 47.500 200 96 4 54 247 19 215 1.18e-54 186

Find 73 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTAAATGAAGACCACTTAA+TGG 0.202475 5.2:+79620722 MS.gene81406:CDS
TCCAATCAGGAGCAAATTTC+AGG 0.224053 5.2:+79620951 MS.gene81406:CDS
CTTCACTCAAATGTGAGATT+TGG 0.265539 5.2:+79620391 MS.gene81406:CDS
TTCACACTCACAACAGGTTT+TGG 0.272566 5.2:-79621047 None:intergenic
TCTAATCCAGAGGAATTTAT+TGG 0.280624 5.2:+79620177 MS.gene81406:CDS
TAGCATAAACATCTTGCTTA+TGG 0.294667 5.2:-79620254 None:intergenic
GAAAATCTTAGTTCAGAAAA+AGG 0.307362 5.2:+79620789 MS.gene81406:CDS
CCAAAGCAAAACCAAGTAAT+TGG 0.309892 5.2:-79620446 None:intergenic
ACCATTCAAAAGTTCTATCT+TGG 0.319634 5.2:+79620059 MS.gene81406:CDS
TTTCTGTCCAATTCAGTATT+AGG 0.324501 5.2:-79620621 None:intergenic
ATATCCTGCTGCACCATCTT+TGG 0.325154 5.2:-79621083 None:intergenic
GAAAGTGGACCAACTAGTTC+AGG 0.360152 5.2:+79621179 MS.gene81406:CDS
TCCTCCTCCATTGATAATCT+TGG 0.364300 5.2:-79620316 None:intergenic
ACCTGAAATTTGCTCCTGAT+TGG 0.370968 5.2:-79620952 None:intergenic
CGGCGCTTCACCCGATGTTA+TGG 0.378961 5.2:+79620584 MS.gene81406:CDS
AGCAATGCCTAATACTGAAT+TGG 0.385474 5.2:+79620614 MS.gene81406:CDS
TGCTGCACCATCTTTGGCTC+TGG 0.397036 5.2:-79621077 None:intergenic
ATAACCTTCGTCTAAGTGTT+AGG 0.404097 5.2:+79620358 MS.gene81406:CDS
TCTAAAGACATTGAGGTCAA+TGG 0.413522 5.2:+79620135 MS.gene81406:CDS
CTCATTCAGCAACGTATTCA+AGG 0.418214 5.2:-79621018 None:intergenic
TATCTTGAAGACCAATTACT+TGG 0.425671 5.2:+79620435 MS.gene81406:CDS
GAACAAAACCTGCTGGTGAA+TGG 0.428263 5.2:-79620542 None:intergenic
GTGAGATTTGGATGCTTAGC+AGG 0.435343 5.2:+79620403 MS.gene81406:CDS
TCAATATTCACACTCACAAC+AGG 0.440373 5.2:-79621053 None:intergenic
GATGGTGCAGCAGGATATAG+TGG 0.440448 5.2:+79621088 MS.gene81406:CDS
CTAGGTGGAGATTCAACCTT+AGG 0.447213 5.2:-79620864 None:intergenic
CCATTCAAAAGTTCTATCTT+GGG 0.458528 5.2:+79620060 MS.gene81406:CDS
TGCTACTAAATCTTCCAATC+AGG 0.468664 5.2:+79620938 MS.gene81406:CDS
GTCAATACCAAGATTATCAA+TGG 0.470375 5.2:+79620309 MS.gene81406:CDS
TGAATCTGTTCAACCTCCTA+AGG 0.483694 5.2:+79620848 MS.gene81406:CDS
TCCGATGTATTGATGAAAGA+TGG 0.484074 5.2:+79620477 MS.gene81406:CDS
ATACTTTGGTACTGATAGCT+TGG 0.493813 5.2:+79620752 MS.gene81406:CDS
AGAGCCAAAGATGGTGCAGC+AGG 0.494831 5.2:+79621079 MS.gene81406:CDS
ATGTTTACATACATCAAGCT+AGG 0.499383 5.2:+79620208 MS.gene81406:CDS
GTTGAGTTTCCTGAACTAGT+TGG 0.500780 5.2:-79621188 None:intergenic
ACTACTCCAATAAATTCCTC+TGG 0.500999 5.2:-79620183 None:intergenic
GATCTATTCCATTCACCAGC+AGG 0.502121 5.2:+79620534 MS.gene81406:CDS
TCCTTTGTCTAAAGACATTG+AGG 0.512174 5.2:+79620128 MS.gene81406:CDS
TGGTGTTGTTTCCTCTTCAG+TGG 0.515128 5.2:+79620893 MS.gene81406:CDS
CTTCCTATGGACTTTGAAAG+TGG 0.518348 5.2:+79621164 MS.gene81406:CDS
GCTGAAATTGCCATAACATC+GGG 0.522915 5.2:-79620594 None:intergenic
TTAATGGTTTCAGAATACTT+TGG 0.535977 5.2:+79620738 MS.gene81406:CDS
AGTATTCTGAAACCATTAAG+TGG 0.538501 5.2:-79620734 None:intergenic
GAATCTCCACCTAGCAGTAA+TGG 0.539721 5.2:+79620873 MS.gene81406:CDS
GAGGTCAATGGAAAAGAAGC+TGG 0.544229 5.2:+79620147 MS.gene81406:CDS
CGAAGGTTATCGTTAAACAC+AGG 0.552324 5.2:-79620345 None:intergenic
ACCAAGATTATCAATGGAGG+AGG 0.555656 5.2:+79620315 MS.gene81406:CDS
AATACTGAATTGGACAGAAA+CGG 0.555790 5.2:+79620624 MS.gene81406:CDS
GGTGGAGATTCAACCTTAGG+AGG 0.563685 5.2:-79620861 None:intergenic
TGAGCTTGCAGCCACTGAAG+AGG 0.564334 5.2:-79620904 None:intergenic
AATGGTGACACAACAGATTG+TGG 0.566043 5.2:-79620042 None:intergenic
AGATTGTAGTTTCTGTTCAG+AGG 0.567533 5.2:-79621210 None:intergenic
GACAACTGAACAAAACCTGC+TGG 0.568428 5.2:-79620549 None:intergenic
CAGTTGTCGCTTGCTTACAA+CGG 0.570997 5.2:+79620564 MS.gene81406:CDS
TGCTGAAATTGCCATAACAT+CGG 0.572348 5.2:-79620595 None:intergenic
TCCATCTTTCATCAATACAT+CGG 0.573677 5.2:-79620478 None:intergenic
GGTCCACTTTCAAAGTCCAT+AGG 0.582924 5.2:-79621167 None:intergenic
AAAAGAGTTGATCTCAAAGA+CGG 0.598346 5.2:+79620984 MS.gene81406:CDS
AAAAGTTCTATCTTGGGACA+TGG 0.598521 5.2:+79620066 MS.gene81406:CDS
ACCTCAATGTCTTTAGACAA+AGG 0.602535 5.2:-79620129 None:intergenic
TATTGAGCCAGAGCCAAAGA+TGG 0.602654 5.2:+79621070 MS.gene81406:CDS
TGCTGGTGAATGGAATAGAT+CGG 0.604836 5.2:-79620532 None:intergenic
TGTTTACATACATCAAGCTA+GGG 0.605167 5.2:+79620209 MS.gene81406:CDS
CGGTCCTAACACTTAGACGA+AGG 0.613041 5.2:-79620362 None:intergenic
GAAACAACACCATTACTGCT+AGG 0.628325 5.2:-79620882 None:intergenic
ACATTTGAGTGAAGAATCAA+CGG 0.638748 5.2:-79620382 None:intergenic
AGATTATCAATGGAGGAGGA+AGG 0.641532 5.2:+79620319 MS.gene81406:CDS
TTCATCAATACATCGGACAA+AGG 0.653321 5.2:-79620471 None:intergenic
AATACCAAGATTATCAATGG+AGG 0.656844 5.2:+79620312 MS.gene81406:CDS
TGAGATTTGGATGCTTAGCA+GGG 0.672371 5.2:+79620404 MS.gene81406:CDS
TGGTGCAGTGTCTAATCCAG+AGG 0.675789 5.2:+79620167 MS.gene81406:CDS
GAAGGTTATCGTTAAACACA+GGG 0.680691 5.2:-79620344 None:intergenic
ACAACACCATTACTGCTAGG+TGG 0.692805 5.2:-79620879 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTACAATTTTTGGATTT+GGG - chr5.2:79620710-79620729 None:intergenic 20.0%
!!! TCATTTACAATTTTTGGATT+TGG - chr5.2:79620711-79620730 None:intergenic 20.0%
! GAAAATCTTAGTTCAGAAAA+AGG + chr5.2:79620789-79620808 MS.gene81406:CDS 25.0%
! TTAATGGTTTCAGAATACTT+TGG + chr5.2:79620738-79620757 MS.gene81406:CDS 25.0%
!! TGGTCTTCATTTACAATTTT+TGG - chr5.2:79620717-79620736 None:intergenic 25.0%
AATACCAAGATTATCAATGG+AGG + chr5.2:79620312-79620331 MS.gene81406:CDS 30.0%
AATACTGAATTGGACAGAAA+CGG + chr5.2:79620624-79620643 MS.gene81406:CDS 30.0%
ACATTTGAGTGAAGAATCAA+CGG - chr5.2:79620385-79620404 None:intergenic 30.0%
ACCATTCAAAAGTTCTATCT+TGG + chr5.2:79620059-79620078 MS.gene81406:CDS 30.0%
ATGTTTACATACATCAAGCT+AGG + chr5.2:79620208-79620227 MS.gene81406:CDS 30.0%
CCATTCAAAAGTTCTATCTT+GGG + chr5.2:79620060-79620079 MS.gene81406:CDS 30.0%
GTCAATACCAAGATTATCAA+TGG + chr5.2:79620309-79620328 MS.gene81406:CDS 30.0%
TAGCATAAACATCTTGCTTA+TGG - chr5.2:79620257-79620276 None:intergenic 30.0%
TATCTTGAAGACCAATTACT+TGG + chr5.2:79620435-79620454 MS.gene81406:CDS 30.0%
TCCATCTTTCATCAATACAT+CGG - chr5.2:79620481-79620500 None:intergenic 30.0%
TCTAATCCAGAGGAATTTAT+TGG + chr5.2:79620177-79620196 MS.gene81406:CDS 30.0%
TGTAAATGAAGACCACTTAA+TGG + chr5.2:79620722-79620741 MS.gene81406:CDS 30.0%
TGTTTACATACATCAAGCTA+GGG + chr5.2:79620209-79620228 MS.gene81406:CDS 30.0%
TTTCTGTCCAATTCAGTATT+AGG - chr5.2:79620624-79620643 None:intergenic 30.0%
! AAAAGAGTTGATCTCAAAGA+CGG + chr5.2:79620984-79621003 MS.gene81406:CDS 30.0%
! AAGATTTTCAGTATCAGTAG+TGG - chr5.2:79620778-79620797 None:intergenic 30.0%
!! AGTATTCTGAAACCATTAAG+TGG - chr5.2:79620737-79620756 None:intergenic 30.0%
!! CTTCATTTTTGCTAATGACT+CGG - chr5.2:79621144-79621163 None:intergenic 30.0%
!!! TTGGTATTGACAGAATTTTC+AGG - chr5.2:79620300-79620319 None:intergenic 30.0%
AAAAGTTCTATCTTGGGACA+TGG + chr5.2:79620066-79620085 MS.gene81406:CDS 35.0%
ACCTCAATGTCTTTAGACAA+AGG - chr5.2:79620132-79620151 None:intergenic 35.0%
ACTACTCCAATAAATTCCTC+TGG - chr5.2:79620186-79620205 None:intergenic 35.0%
AGATTGTAGTTTCTGTTCAG+AGG - chr5.2:79621213-79621232 None:intergenic 35.0%
AGCAAAAATGAAGCTTCCTA+TGG + chr5.2:79621151-79621170 MS.gene81406:CDS 35.0%
AGCAATGCCTAATACTGAAT+TGG + chr5.2:79620614-79620633 MS.gene81406:CDS 35.0%
ATAACCTTCGTCTAAGTGTT+AGG + chr5.2:79620358-79620377 MS.gene81406:CDS 35.0%
CCAAAGCAAAACCAAGTAAT+TGG - chr5.2:79620449-79620468 None:intergenic 35.0%
CTTCACTCAAATGTGAGATT+TGG + chr5.2:79620391-79620410 MS.gene81406:CDS 35.0%
GAAGGTTATCGTTAAACACA+GGG - chr5.2:79620347-79620366 None:intergenic 35.0%
TCAATATTCACACTCACAAC+AGG - chr5.2:79621056-79621075 None:intergenic 35.0%
TCCTTTGTCTAAAGACATTG+AGG + chr5.2:79620128-79620147 MS.gene81406:CDS 35.0%
TCTAAAGACATTGAGGTCAA+TGG + chr5.2:79620135-79620154 MS.gene81406:CDS 35.0%
TGCTACTAAATCTTCCAATC+AGG + chr5.2:79620938-79620957 MS.gene81406:CDS 35.0%
TGCTGAAATTGCCATAACAT+CGG - chr5.2:79620598-79620617 None:intergenic 35.0%
TTCATCAATACATCGGACAA+AGG - chr5.2:79620474-79620493 None:intergenic 35.0%
! AGTAACTCACGTGTGTTTTA+TGG + chr5.2:79621242-79621261 MS.gene81406:CDS 35.0%
! CCCAAGATAGAACTTTTGAA+TGG - chr5.2:79620063-79620082 None:intergenic 35.0%
! TCCGATGTATTGATGAAAGA+TGG + chr5.2:79620477-79620496 MS.gene81406:CDS 35.0%
!! AGAAAAAGGTGTTCAACTTG+TGG + chr5.2:79620803-79620822 MS.gene81406:CDS 35.0%
!! ATACTTTGGTACTGATAGCT+TGG + chr5.2:79620752-79620771 MS.gene81406:CDS 35.0%
!!! CCAATTACTTGGTTTTGCTT+TGG + chr5.2:79620446-79620465 MS.gene81406:CDS 35.0%
AATGGTGACACAACAGATTG+TGG - chr5.2:79620045-79620064 None:intergenic 40.0%
ACCAAGATTATCAATGGAGG+AGG + chr5.2:79620315-79620334 MS.gene81406:CDS 40.0%
AGATTATCAATGGAGGAGGA+AGG + chr5.2:79620319-79620338 MS.gene81406:CDS 40.0%
CGAAGGTTATCGTTAAACAC+AGG - chr5.2:79620348-79620367 None:intergenic 40.0%
CTCATTCAGCAACGTATTCA+AGG - chr5.2:79621021-79621040 None:intergenic 40.0%
GAAACAACACCATTACTGCT+AGG - chr5.2:79620885-79620904 None:intergenic 40.0%
GCTGAAATTGCCATAACATC+GGG - chr5.2:79620597-79620616 None:intergenic 40.0%
GTTGAGTTTCCTGAACTAGT+TGG - chr5.2:79621191-79621210 None:intergenic 40.0%
TCCAATCAGGAGCAAATTTC+AGG + chr5.2:79620951-79620970 MS.gene81406:CDS 40.0%
TGAATCTGTTCAACCTCCTA+AGG + chr5.2:79620848-79620867 MS.gene81406:CDS 40.0%
TGAGATTTGGATGCTTAGCA+GGG + chr5.2:79620404-79620423 MS.gene81406:CDS 40.0%
TGCTGGTGAATGGAATAGAT+CGG - chr5.2:79620535-79620554 None:intergenic 40.0%
! ACCTGAAATTTGCTCCTGAT+TGG - chr5.2:79620955-79620974 None:intergenic 40.0%
! CTTCCTATGGACTTTGAAAG+TGG + chr5.2:79621164-79621183 MS.gene81406:CDS 40.0%
! TCCTCCTCCATTGATAATCT+TGG - chr5.2:79620319-79620338 None:intergenic 40.0%
! TTCACACTCACAACAGGTTT+TGG - chr5.2:79621050-79621069 None:intergenic 40.0%
!!! GCTTTTTTCGGAGTCTTTCT+CGG - chr5.2:79620652-79620671 None:intergenic 40.0%
ACAACACCATTACTGCTAGG+TGG - chr5.2:79620882-79620901 None:intergenic 45.0%
ATATCCTGCTGCACCATCTT+TGG - chr5.2:79621086-79621105 None:intergenic 45.0%
CTAGGTGGAGATTCAACCTT+AGG - chr5.2:79620867-79620886 None:intergenic 45.0%
GAAAGTGGACCAACTAGTTC+AGG + chr5.2:79621179-79621198 MS.gene81406:CDS 45.0%
GAACAAAACCTGCTGGTGAA+TGG - chr5.2:79620545-79620564 None:intergenic 45.0%
GAATCTCCACCTAGCAGTAA+TGG + chr5.2:79620873-79620892 MS.gene81406:CDS 45.0%
GACAACTGAACAAAACCTGC+TGG - chr5.2:79620552-79620571 None:intergenic 45.0%
GAGGTCAATGGAAAAGAAGC+TGG + chr5.2:79620147-79620166 MS.gene81406:CDS 45.0%
GATCTATTCCATTCACCAGC+AGG + chr5.2:79620534-79620553 MS.gene81406:CDS 45.0%
GGTCCACTTTCAAAGTCCAT+AGG - chr5.2:79621170-79621189 None:intergenic 45.0%
GTGAGATTTGGATGCTTAGC+AGG + chr5.2:79620403-79620422 MS.gene81406:CDS 45.0%
TATTGAGCCAGAGCCAAAGA+TGG + chr5.2:79621070-79621089 MS.gene81406:CDS 45.0%
! CAGTTGTCGCTTGCTTACAA+CGG + chr5.2:79620564-79620583 MS.gene81406:CDS 45.0%
!! TGGTGTTGTTTCCTCTTCAG+TGG + chr5.2:79620893-79620912 MS.gene81406:CDS 45.0%
AAAAAGCGAGTCGCTCGTGA+AGG + chr5.2:79620665-79620684 MS.gene81406:CDS 50.0%
CGGTCCTAACACTTAGACGA+AGG - chr5.2:79620365-79620384 None:intergenic 50.0%
GATGGTGCAGCAGGATATAG+TGG + chr5.2:79621088-79621107 MS.gene81406:CDS 50.0%
GGTGGAGATTCAACCTTAGG+AGG - chr5.2:79620864-79620883 None:intergenic 50.0%
TGGTGCAGTGTCTAATCCAG+AGG + chr5.2:79620167-79620186 MS.gene81406:CDS 50.0%
!!! CACGAGCGACTCGCTTTTTT+CGG - chr5.2:79620664-79620683 None:intergenic 50.0%
AGAGCCAAAGATGGTGCAGC+AGG + chr5.2:79621079-79621098 MS.gene81406:CDS 55.0%
TGAGCTTGCAGCCACTGAAG+AGG - chr5.2:79620907-79620926 None:intergenic 55.0%
! TGCTGCACCATCTTTGGCTC+TGG - chr5.2:79621080-79621099 None:intergenic 55.0%
CGGCGCTTCACCCGATGTTA+TGG + chr5.2:79620584-79620603 MS.gene81406:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 79620033 79621283 79620033 ID=MS.gene81406
chr5.2 mRNA 79620033 79621283 79620033 ID=MS.gene81406.t1;Parent=MS.gene81406
chr5.2 exon 79620033 79621283 79620033 ID=MS.gene81406.t1.exon1;Parent=MS.gene81406.t1
chr5.2 CDS 79620033 79621283 79620033 ID=cds.MS.gene81406.t1;Parent=MS.gene81406.t1
Gene Sequence

>MS.gene81406

ATGGAATCTCCACAATCTGTTGTGTCACCATTCAAAAGTTCTATCTTGGGACATGGTGAGAAGTATAAGTCTGATGTTTTAACACAAAGCTCTAGTCCTTTGTCTAAAGACATTGAGGTCAATGGAAAAGAAGCTGGTGCAGTGTCTAATCCAGAGGAATTTATTGGAGTAGTTGATGTTTACATACATCAAGCTAGGGACATTCATAACATCTGCATATACCATAAGCAAGATGTTTATGCTAAGATTTCTTTGACTAGTGAACCTGAAAATTCTGTCAATACCAAGATTATCAATGGAGGAGGAAGGAACCCTGTGTTTAACGATAACCTTCGTCTAAGTGTTAGGACCGTTGATTCTTCACTCAAATGTGAGATTTGGATGCTTAGCAGGGTGAAGAACTATCTTGAAGACCAATTACTTGGTTTTGCTTTGGTACCTTTGTCCGATGTATTGATGAAAGATGGAAAACTAGAGAAAGAGTTCTCTCTTTCTTCGACCGATCTATTCCATTCACCAGCAGGTTTTGTTCAGTTGTCGCTTGCTTACAACGGCGCTTCACCCGATGTTATGGCAATTTCAGCAATGCCTAATACTGAATTGGACAGAAACGGCACCGAGAAAGACTCCGAAAAAAGCGAGTCGCTCGTGAAGGATTTAGATAAAATAGAGTTCCCAAATCCAAAAATTGTAAATGAAGACCACTTAATGGTTTCAGAATACTTTGGTACTGATAGCTTGGCCACTACTGATACTGAAAATCTTAGTTCAGAAAAAGGTGTTCAACTTGTGGAAAGCTTCTCAGCATGTAGTGTTGAATCTGTTCAACCTCCTAAGGTTGAATCTCCACCTAGCAGTAATGGTGTTGTTTCCTCTTCAGTGGCTGCAAGCTCAGAATCACATGCTGCTACTAAATCTTCCAATCAGGAGCAAATTTCAGGTACAGAAGAGAAAAGAGTTGATCTCAAAGACGGCGAGAGTGATTCCTTGAATACGTTGCTGAATGAGTCATTTCCAAAACCTGTTGTGAGTGTGAATATTGAGCCAGAGCCAAAGATGGTGCAGCAGGATATAGTGGACATGTACTTGAAAAGTATGCAGCAATTTACCGAGTCATTAGCAAAAATGAAGCTTCCTATGGACTTTGAAAGTGGACCAACTAGTTCAGGAAACTCAACCTCTGAACAGAAACTACAATCTTCAAAGAGTAGTAACTCACGTGTGTTTTATGGTAGCAGAGCTTTCTTCTGA

Protein sequence

>MS.gene81406.t1

MESPQSVVSPFKSSILGHGEKYKSDVLTQSSSPLSKDIEVNGKEAGAVSNPEEFIGVVDVYIHQARDIHNICIYHKQDVYAKISLTSEPENSVNTKIINGGGRNPVFNDNLRLSVRTVDSSLKCEIWMLSRVKNYLEDQLLGFALVPLSDVLMKDGKLEKEFSLSSTDLFHSPAGFVQLSLAYNGASPDVMAISAMPNTELDRNGTEKDSEKSESLVKDLDKIEFPNPKIVNEDHLMVSEYFGTDSLATTDTENLSSEKGVQLVESFSACSVESVQPPKVESPPSSNGVVSSSVAASSESHAATKSSNQEQISGTEEKRVDLKDGESDSLNTLLNESFPKPVVSVNIEPEPKMVQQDIVDMYLKSMQQFTESLAKMKLPMDFESGPTSSGNSTSEQKLQSSKSSNSRVFYGSRAFF