Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038754.t1 | XP_024629315.1 | 84.3 | 470 | 66 | 4 | 1 | 470 | 1 | 462 | 1.60E-217 | 765.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038754.t1 | Q557M8 | 22.4 | 393 | 222 | 10 | 8 | 382 | 14 | 341 | 1.5e-11 | 72.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038754.t1 | A0A396IJ19 | 87.7 | 470 | 58 | 0 | 1 | 470 | 1 | 470 | 4.6e-211 | 743.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene038754 | MS.gene08242 | PPI |
| MS.gene038754 | MS.gene39443 | PPI |
| MS.gene31944 | MS.gene038754 | PPI |
| MS.gene038754 | MS.gene33782 | PPI |
| MS.gene031871 | MS.gene038754 | PPI |
| MS.gene007950 | MS.gene038754 | PPI |
| MS.gene038754 | MS.gene052488 | PPI |
| MS.gene038754 | MS.gene007951 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038754.t1 | MTR_8g077000 | 84.189 | 487 | 52 | 6 | 1 | 470 | 1 | 479 | 0.0 | 776 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038754.t1 | AT1G03280 | 58.710 | 465 | 160 | 13 | 1 | 451 | 12 | 458 | 1.21e-177 | 506 |
| MS.gene038754.t1 | AT1G03280 | 58.710 | 465 | 160 | 13 | 1 | 451 | 12 | 458 | 1.21e-177 | 506 |
| MS.gene038754.t1 | AT1G03280 | 58.710 | 465 | 160 | 13 | 1 | 451 | 12 | 458 | 1.21e-177 | 506 |
| MS.gene038754.t1 | AT4G20340 | 52.586 | 464 | 184 | 14 | 1 | 451 | 13 | 453 | 1.35e-144 | 422 |
| MS.gene038754.t1 | AT4G20340 | 52.586 | 464 | 184 | 14 | 1 | 451 | 13 | 453 | 1.35e-144 | 422 |
| MS.gene038754.t1 | AT4G20810 | 47.458 | 472 | 181 | 16 | 8 | 469 | 1 | 415 | 5.66e-130 | 383 |
| MS.gene038754.t1 | AT4G20340 | 52.998 | 417 | 161 | 14 | 48 | 451 | 4 | 398 | 2.62e-127 | 376 |
Find 101 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATCACTTCTTCCAGTTT+TGG | 0.162141 | 8.3:-14211152 | None:intergenic |
| AACTGACGGTACATCTTTAA+AGG | 0.182546 | 8.3:+14216689 | MS.gene038754:CDS |
| GACTTGCCTGTTCCAGAATT+TGG | 0.189652 | 8.3:+14214452 | MS.gene038754:CDS |
| TCGTCCTCGTCGTCTTCTTC+TGG | 0.291126 | 8.3:-14218954 | None:intergenic |
| CATCTGCTGCGTTCGCATCT+AGG | 0.312497 | 8.3:-14217084 | None:intergenic |
| GGGCTGATTATCTCCTTTGC+TGG | 0.323516 | 8.3:-14211131 | None:intergenic |
| GCTTGAAGACTACCAAATTC+TGG | 0.326095 | 8.3:-14214464 | None:intergenic |
| AAATCTCATCTGGGATATAA+TGG | 0.332016 | 8.3:+14214548 | MS.gene038754:CDS |
| GAGAAATTGGTACTTGTTGT+AGG | 0.340168 | 8.3:-14210977 | None:intergenic |
| CGGTGATTCTAAATCTCATC+TGG | 0.343831 | 8.3:+14214538 | MS.gene038754:CDS |
| GGAGATAATCAGCCCAAAAC+TGG | 0.349455 | 8.3:+14211139 | MS.gene038754:CDS |
| ACGACGGCGTGAAAAGTTAA+AGG | 0.370019 | 8.3:+14212963 | MS.gene038754:CDS |
| TGGGAAGAGGCTCCTGTTAG+AGG | 0.378747 | 8.3:+14217181 | MS.gene038754:CDS |
| GAAGCCCGAGCTAGTGCAGC+TGG | 0.384987 | 8.3:+14214491 | MS.gene038754:CDS |
| ATCTGCTGCGTTCGCATCTA+GGG | 0.389807 | 8.3:-14217083 | None:intergenic |
| AGAGAACAAGAACACCATTC+AGG | 0.401351 | 8.3:+14212653 | MS.gene038754:CDS |
| CACGCCCAGCTGCACTAGCT+CGG | 0.403153 | 8.3:-14214496 | None:intergenic |
| GCTTAAGAAGCAACGCGAAT+TGG | 0.412214 | 8.3:+14217042 | MS.gene038754:CDS |
| TTATTTCAGATACAATGCTT+TGG | 0.415674 | 8.3:+14212804 | MS.gene038754:intron |
| GGCTGAAGTGTACCTCTAAC+AGG | 0.418995 | 8.3:-14217193 | None:intergenic |
| GCAGCTGGGCGTGCAGCTAA+TGG | 0.419366 | 8.3:+14214506 | MS.gene038754:CDS |
| GTTGCTTTGTATGCAATTTG+AGG | 0.441479 | 8.3:-14211345 | None:intergenic |
| ATTTGGTAGTCTTCAAGCTT+GGG | 0.444477 | 8.3:+14214469 | MS.gene038754:CDS |
| ACGCCCAGCTGCACTAGCTC+GGG | 0.452951 | 8.3:-14214495 | None:intergenic |
| CGGTTGGAAGTACTGCTAGA+AGG | 0.463810 | 8.3:-14217109 | None:intergenic |
| AGGAAAAGAGGGAGAAGAAA+AGG | 0.464405 | 8.3:+14212349 | MS.gene038754:CDS |
| ATAAGTTAGCTGCTCAAGAC+GGG | 0.467809 | 8.3:+14212905 | MS.gene038754:CDS |
| AGATTATCACTAGAGATCAT+AGG | 0.477747 | 8.3:+14211401 | MS.gene038754:CDS |
| AATTTGGTAGTCTTCAAGCT+TGG | 0.481156 | 8.3:+14214468 | MS.gene038754:CDS |
| TTCCACTGTGAACGTTGTAA+TGG | 0.482559 | 8.3:+14212862 | MS.gene038754:CDS |
| AGACTACCAAATTCTGGAAC+AGG | 0.482675 | 8.3:-14214458 | None:intergenic |
| AAAGGACATGCTTCAGAAGA+TGG | 0.491664 | 8.3:+14212981 | MS.gene038754:CDS |
| CCTCCATGGATGATTACATC+AGG | 0.493168 | 8.3:+14216717 | MS.gene038754:CDS |
| AAATGCAATTGGAGCTTACA+AGG | 0.494474 | 8.3:+14218902 | MS.gene038754:CDS |
| TGTACATATATATTCCTGAA+TGG | 0.494787 | 8.3:-14212667 | None:intergenic |
| TTGTTCTTGACGCGCTCACC+AGG | 0.495221 | 8.3:+14211191 | MS.gene038754:CDS |
| ACAGCGTGGAGAGGTGAAGC+AGG | 0.499690 | 8.3:+14216758 | MS.gene038754:CDS |
| TATGTACAAACTGTGGCAAA+AGG | 0.503511 | 8.3:+14212682 | MS.gene038754:CDS |
| TGAAGCTACGACAGGAAAAG+AGG | 0.510943 | 8.3:+14212337 | MS.gene038754:CDS |
| AGAAATTGGTACTTGTTGTA+GGG | 0.511459 | 8.3:-14210976 | None:intergenic |
| ACCTTGGTATCTCCGCTGTA+TGG | 0.515063 | 8.3:-14214581 | None:intergenic |
| GTCTTCTTCTGGCTGAGCAT+CGG | 0.526811 | 8.3:-14218943 | None:intergenic |
| GACGATATCGACTGGGAGGA+AGG | 0.528222 | 8.3:+14218972 | MS.gene038754:CDS |
| CGACTGACTTGATCTACTAA+AGG | 0.542394 | 8.3:-14214422 | None:intergenic |
| TGATGGTACCGAGTGGGAAG+AGG | 0.544924 | 8.3:+14217168 | MS.gene038754:CDS |
| ACGACGAGGACGATATCGAC+TGG | 0.550692 | 8.3:+14218964 | MS.gene038754:CDS |
| ATACCTGATGTAATCATCCA+TGG | 0.551552 | 8.3:-14216720 | None:intergenic |
| TTGATTTCAAGCCAACTTGG+CGG | 0.551942 | 8.3:-14217129 | None:intergenic |
| TTTCAAGCCAACTTGGCGGT+TGG | 0.553993 | 8.3:-14217125 | None:intergenic |
| CAGATGAGATTTAGAATCAC+CGG | 0.557188 | 8.3:-14214537 | None:intergenic |
| TTTAGTAGATCAAGTCAGTC+GGG | 0.559290 | 8.3:+14214424 | MS.gene038754:CDS |
| CAGGTGGTAGTTGACTTCAA+TGG | 0.563361 | 8.3:+14216633 | MS.gene038754:intron |
| GATAAGTTAGCTGCTCAAGA+CGG | 0.564672 | 8.3:+14212904 | MS.gene038754:CDS |
| TTTATAGGATGAGTATGTCA+AGG | 0.564885 | 8.3:+14217003 | MS.gene038754:intron |
| TATGACGATCTTACCAGCAA+AGG | 0.565230 | 8.3:+14211118 | MS.gene038754:CDS |
| AGTACTTCCAACCGCCAAGT+TGG | 0.569471 | 8.3:+14217118 | MS.gene038754:CDS |
| TGCAGATATATGATGTGGTT+AGG | 0.570661 | 8.3:+14212589 | MS.gene038754:intron |
| AACAGGAGCCTCTTCCCACT+CGG | 0.576584 | 8.3:-14217176 | None:intergenic |
| GTATCTCCGCTGTATGGCAA+TGG | 0.577878 | 8.3:-14214575 | None:intergenic |
| AAGCCCGAGCTAGTGCAGCT+GGG | 0.582139 | 8.3:+14214492 | MS.gene038754:CDS |
| AAACGTGAGGAAGATGATGA+TGG | 0.590028 | 8.3:+14217151 | MS.gene038754:CDS |
| CTTTAGTAGATCAAGTCAGT+CGG | 0.591765 | 8.3:+14214423 | MS.gene038754:CDS |
| GTTGACTTCAATGGTACTGA+AGG | 0.599548 | 8.3:+14216642 | MS.gene038754:CDS |
| GGTACTGAAGGCAAAGGAGA+GGG | 0.602003 | 8.3:+14216654 | MS.gene038754:CDS |
| GTCTGTGAATTGCACTGATG+TGG | 0.605194 | 8.3:-14216808 | None:intergenic |
| GAAGCTACGACAGGAAAAGA+GGG | 0.609436 | 8.3:+14212338 | MS.gene038754:CDS |
| GCAGCTAATGGAGATAATGC+CGG | 0.610543 | 8.3:+14214518 | MS.gene038754:CDS |
| ATGGGTTAGAGAAGAAGACT+TGG | 0.611007 | 8.3:+14211315 | MS.gene038754:CDS |
| GCCATACAGCGGAGATACCA+AGG | 0.618161 | 8.3:+14214580 | MS.gene038754:CDS |
| CGACGAGGACGATATCGACT+GGG | 0.621959 | 8.3:+14218965 | MS.gene038754:CDS |
| CACTAGAGATCATAGGAGAG+AGG | 0.622575 | 8.3:+14211408 | MS.gene038754:CDS |
| CTGCTGTCTAGATTATGCAC+AGG | 0.622608 | 8.3:+14212394 | MS.gene038754:CDS |
| GCTACAGCTGAAGCTACGAC+AGG | 0.624340 | 8.3:+14212329 | MS.gene038754:CDS |
| TCTGCTGCGTTCGCATCTAG+GGG | 0.626633 | 8.3:-14217082 | None:intergenic |
| GGTGATTCTAAATCTCATCT+GGG | 0.628231 | 8.3:+14214539 | MS.gene038754:CDS |
| AGATGATGATGGTACCGAGT+GGG | 0.630560 | 8.3:+14217162 | MS.gene038754:CDS |
| TTCAATGGTACTGAAGGCAA+AGG | 0.631073 | 8.3:+14216648 | MS.gene038754:CDS |
| TAAGTTAGCTGCTCAAGACG+GGG | 0.634311 | 8.3:+14212906 | MS.gene038754:CDS |
| GAGCGCGTCAAGAACAACTA+CGG | 0.640458 | 8.3:-14211185 | None:intergenic |
| TGGTACTGAAGGCAAAGGAG+AGG | 0.643856 | 8.3:+14216653 | MS.gene038754:CDS |
| ATGAGAATGCACGAAGACGA+CGG | 0.644965 | 8.3:+14212947 | MS.gene038754:CDS |
| TACGAAAGAACAGCGTGGAG+AGG | 0.645484 | 8.3:+14216749 | MS.gene038754:CDS |
| TTTGTTGCAGATATATGATG+TGG | 0.649334 | 8.3:+14212584 | MS.gene038754:intron |
| AGCAACTTCGTCGAACTCTG+CGG | 0.650459 | 8.3:+14211362 | MS.gene038754:CDS |
| GAATATATATGTACAAACTG+TGG | 0.654824 | 8.3:+14212675 | MS.gene038754:CDS |
| TGGCTTGAAATCAAAACGTG+AGG | 0.659414 | 8.3:+14217138 | MS.gene038754:CDS |
| TAGAGAAGAAGACTTGGCAA+AGG | 0.661782 | 8.3:+14211321 | MS.gene038754:CDS |
| GGTGTTAAATCTGAAACTGA+CGG | 0.664295 | 8.3:+14216675 | MS.gene038754:CDS |
| TGCAATTGGAGCTTACAAGG+TGG | 0.682571 | 8.3:+14218905 | MS.gene038754:CDS |
| AAGTTAGCTGCTCAAGACGG+GGG | 0.688054 | 8.3:+14212907 | MS.gene038754:CDS |
| GGACATGCTTCAGAAGATGG+AGG | 0.689485 | 8.3:+14212984 | MS.gene038754:CDS |
| ACGTTGTAATGGAAAACTAG+AGG | 0.692315 | 8.3:+14212873 | MS.gene038754:CDS |
| GGAGCACCATTGCCATACAG+CGG | 0.718354 | 8.3:+14214569 | MS.gene038754:CDS |
| AAGATGATGATGGTACCGAG+TGG | 0.721118 | 8.3:+14217161 | MS.gene038754:CDS |
| ACTGGAAGAAGTGATAACAG+AGG | 0.728511 | 8.3:+14211157 | MS.gene038754:CDS |
| TTCCATTACAACGTTCACAG+TGG | 0.729270 | 8.3:-14212864 | None:intergenic |
| CGAGGACGATATCGACTGGG+AGG | 0.742122 | 8.3:+14218968 | MS.gene038754:CDS |
| CCTGATGTAATCATCCATGG+AGG | 0.742710 | 8.3:-14216717 | None:intergenic |
| AATCTTACGAAAGAACAGCG+TGG | 0.751641 | 8.3:+14216744 | MS.gene038754:CDS |
| TCAGCCAGAAGAAGACGACG+AGG | 0.751885 | 8.3:+14218950 | MS.gene038754:CDS |
| ACAATGCTTTGGATGCACTG+CGG | 0.757429 | 8.3:+14212815 | MS.gene038754:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 14210967 | 14218998 | 14210967 | ID=MS.gene038754 |
| chr8.3 | mRNA | 14210967 | 14218998 | 14210967 | ID=MS.gene038754.t1;Parent=MS.gene038754 |
| chr8.3 | exon | 14210967 | 14210986 | 14210967 | ID=MS.gene038754.t1.exon1;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14210967 | 14210986 | 14210967 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| chr8.3 | exon | 14211090 | 14211212 | 14211090 | ID=MS.gene038754.t1.exon2;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14211090 | 14211212 | 14211090 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| chr8.3 | exon | 14211309 | 14211429 | 14211309 | ID=MS.gene038754.t1.exon3;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14211309 | 14211429 | 14211309 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| chr8.3 | exon | 14212290 | 14212415 | 14212290 | ID=MS.gene038754.t1.exon4;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14212290 | 14212415 | 14212290 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| chr8.3 | exon | 14212594 | 14212703 | 14212594 | ID=MS.gene038754.t1.exon5;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14212594 | 14212703 | 14212594 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| chr8.3 | exon | 14212813 | 14213005 | 14212813 | ID=MS.gene038754.t1.exon6;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14212813 | 14213005 | 14212813 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| chr8.3 | exon | 14214410 | 14214601 | 14214410 | ID=MS.gene038754.t1.exon7;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14214410 | 14214601 | 14214410 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| chr8.3 | exon | 14216636 | 14216872 | 14216636 | ID=MS.gene038754.t1.exon8;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14216636 | 14216872 | 14216636 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| chr8.3 | exon | 14217010 | 14217202 | 14217010 | ID=MS.gene038754.t1.exon9;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14217010 | 14217202 | 14217010 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| chr8.3 | exon | 14218901 | 14218998 | 14218901 | ID=MS.gene038754.t1.exon10;Parent=MS.gene038754.t1 |
| chr8.3 | CDS | 14218901 | 14218998 | 14218901 | ID=cds.MS.gene038754.t1;Parent=MS.gene038754.t1 |
| Gene Sequence |
| Protein sequence |
