Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038844.t1 | XP_013463904.1 | 98.7 | 377 | 5 | 0 | 1 | 377 | 1 | 377 | 9.60E-218 | 765.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038844.t1 | O81024 | 62.4 | 372 | 138 | 2 | 1 | 372 | 1 | 370 | 6.0e-136 | 485.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038844.t1 | A0A072V7D3 | 98.7 | 377 | 5 | 0 | 1 | 377 | 1 | 377 | 6.9e-218 | 765.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene038844 | MS.gene046260 | PPI |
| MS.gene016513 | MS.gene038844 | PPI |
| MS.gene038844 | MS.gene87205 | PPI |
| MS.gene038844 | MS.gene063832 | PPI |
| MS.gene038844 | MS.gene039577 | PPI |
| MS.gene038844 | MS.gene069952 | PPI |
| MS.gene038844 | MS.gene88406 | PPI |
| MS.gene069299 | MS.gene038844 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038844.t1 | MTR_2g054370 | 98.674 | 377 | 5 | 0 | 1 | 377 | 1 | 377 | 0.0 | 775 |
| MS.gene038844.t1 | MTR_6g039820 | 34.365 | 323 | 189 | 6 | 59 | 366 | 29 | 343 | 7.02e-55 | 184 |
| MS.gene038844.t1 | MTR_3g013430 | 34.375 | 320 | 195 | 5 | 56 | 366 | 140 | 453 | 8.10e-55 | 188 |
| MS.gene038844.t1 | MTR_8g041330 | 33.841 | 328 | 202 | 6 | 48 | 366 | 41 | 362 | 8.12e-55 | 186 |
| MS.gene038844.t1 | MTR_1g097850 | 33.231 | 325 | 178 | 8 | 57 | 365 | 27 | 328 | 4.60e-49 | 169 |
| MS.gene038844.t1 | MTR_6g040230 | 35.897 | 234 | 133 | 4 | 142 | 366 | 271 | 496 | 9.93e-40 | 147 |
| MS.gene038844.t1 | MTR_1g097850 | 31.276 | 243 | 133 | 6 | 57 | 285 | 27 | 249 | 3.90e-30 | 116 |
| MS.gene038844.t1 | MTR_8g041220 | 35.955 | 89 | 50 | 2 | 267 | 349 | 40 | 127 | 6.37e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene038844.t1 | AT2G26830 | 63.610 | 349 | 127 | 0 | 24 | 372 | 22 | 370 | 1.29e-170 | 481 |
| MS.gene038844.t1 | AT4G09760 | 35.256 | 312 | 186 | 6 | 64 | 366 | 38 | 342 | 1.35e-55 | 186 |
| MS.gene038844.t1 | AT4G09760 | 35.256 | 312 | 186 | 6 | 64 | 366 | 38 | 342 | 1.35e-55 | 186 |
| MS.gene038844.t1 | AT1G74320 | 32.515 | 326 | 197 | 9 | 56 | 368 | 32 | 347 | 7.70e-54 | 181 |
| MS.gene038844.t1 | AT1G71697 | 29.480 | 346 | 224 | 7 | 31 | 365 | 8 | 344 | 1.66e-47 | 165 |
| MS.gene038844.t1 | AT4G09760 | 30.208 | 192 | 126 | 4 | 64 | 253 | 38 | 223 | 6.11e-22 | 94.4 |
| MS.gene038844.t1 | AT4G09760 | 29.143 | 175 | 116 | 4 | 64 | 236 | 38 | 206 | 5.79e-18 | 82.0 |
Find 73 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAGATATCATTCCATAAT+TGG | 0.099867 | 2.1:-36611697 | None:intergenic |
| TGATATTGTTCGTTCATATT+TGG | 0.100547 | 2.1:-36618300 | None:intergenic |
| TCTCACAATGACTTGCTTTC+TGG | 0.116891 | 2.1:+36617258 | MS.gene038844:CDS |
| TTGCAGTTGTTTGGCTATTT+TGG | 0.127146 | 2.1:-36611636 | None:intergenic |
| GCAGCATCTGTTCTAGAATT+TGG | 0.157391 | 2.1:+36613037 | MS.gene038844:intron |
| TTTAAAGTTGAGAAAATTTC+TGG | 0.169623 | 2.1:+36609144 | MS.gene038844:CDS |
| GCTAAGTGGCTTGGAATATT+TGG | 0.191747 | 2.1:+36610520 | MS.gene038844:CDS |
| CCCAATTATGGAATGATATC+TGG | 0.258364 | 2.1:+36611696 | MS.gene038844:CDS |
| TCTACTCATTCCTTTCTAAA+AGG | 0.267387 | 2.1:-36619945 | None:intergenic |
| GATATTGTTCGTTCATATTT+GGG | 0.276266 | 2.1:-36618299 | None:intergenic |
| CCAGATATCATTCCATAATT+GGG | 0.278940 | 2.1:-36611696 | None:intergenic |
| GTTCTAAGGAACCCCAATTA+TGG | 0.284270 | 2.1:+36611684 | MS.gene038844:CDS |
| TACATCACTGCTGCAGGATT+TGG | 0.299530 | 2.1:+36610496 | MS.gene038844:CDS |
| CAAGATAATCAAAATCTATT+GGG | 0.308596 | 2.1:-36619852 | None:intergenic |
| CCAAGATAATCAAAATCTAT+TGG | 0.314209 | 2.1:-36619853 | None:intergenic |
| TCAATGATATGCTCGGTGTT+GGG | 0.351100 | 2.1:-36609486 | None:intergenic |
| TTCAAGACCTGGTCAAGTTT+AGG | 0.351629 | 2.1:+36609111 | MS.gene038844:CDS |
| TGTGGAAATTCCTGGTTCTA+AGG | 0.364183 | 2.1:+36611670 | MS.gene038844:CDS |
| ACGAACAATATCATTTCTTC+AGG | 0.383658 | 2.1:+36618310 | MS.gene038844:CDS |
| GCTTGTTCTGCGATTTCAAC+GGG | 0.403905 | 2.1:-36608486 | None:intergenic |
| TGATCGCTTTCGAGAATTGC+AGG | 0.404652 | 2.1:+36609506 | MS.gene038844:CDS |
| ATTACCTCTTGAGGTCTGTC+TGG | 0.424572 | 2.1:-36618345 | None:intergenic |
| TGGCTTGGAATATTTGGAAA+TGG | 0.427165 | 2.1:+36610526 | MS.gene038844:CDS |
| ATCAATGATATGCTCGGTGT+TGG | 0.428005 | 2.1:-36609487 | None:intergenic |
| ATTATAACCATCAGATTGTA+TGG | 0.431135 | 2.1:+36609462 | MS.gene038844:CDS |
| TTTCACGGTGTGGAAATTCC+TGG | 0.457909 | 2.1:+36611662 | MS.gene038844:CDS |
| ATCAAATACATCACTGCTGC+AGG | 0.459460 | 2.1:+36610490 | MS.gene038844:CDS |
| AAGGGAAAGAGAAGGATCAA+TGG | 0.463105 | 2.1:-36608550 | None:intergenic |
| TTTGTCAGTACTGAAGGTTT+CGG | 0.467242 | 2.1:+36609413 | MS.gene038844:intron |
| TGGGTGGAAAAGTTAAGATC+TGG | 0.470594 | 2.1:+36608460 | MS.gene038844:CDS |
| GCGCATACTCTTGCTCCATC+AGG | 0.478124 | 2.1:+36610571 | MS.gene038844:CDS |
| AGATATGCGAGAACCAAAAC+TGG | 0.481384 | 2.1:+36611610 | MS.gene038844:intron |
| TTCGATACAACGAGTACAAA+AGG | 0.487153 | 2.1:+36619887 | MS.gene038844:CDS |
| AGCTTGTTCTGCGATTTCAA+CGG | 0.491199 | 2.1:-36608487 | None:intergenic |
| TTACAAGACAGTTTCATTCA+AGG | 0.491822 | 2.1:+36613081 | MS.gene038844:CDS |
| AACTACAGAGGCTATGACAT+AGG | 0.495161 | 2.1:+36618114 | MS.gene038844:CDS |
| TTATTATTTGTCAGTACTGA+AGG | 0.496047 | 2.1:+36609407 | MS.gene038844:intron |
| TCTTAAAACTTTGTATGTTG+AGG | 0.497216 | 2.1:+36619675 | MS.gene038844:CDS |
| TGATGAAATTTATGAGCTCC+AGG | 0.497219 | 2.1:+36613111 | MS.gene038844:CDS |
| TGCAGCAGTGATGTATTTGA+TGG | 0.502189 | 2.1:-36610488 | None:intergenic |
| GGATCAATGGAAAGAAGAGA+AGG | 0.511878 | 2.1:-36608537 | None:intergenic |
| CTGAAGGTTTCGGTTAAACA+AGG | 0.517757 | 2.1:+36609423 | MS.gene038844:CDS |
| TTGAAATCGCAGAACAAGCT+CGG | 0.518498 | 2.1:+36608490 | MS.gene038844:CDS |
| CAATGATATGCTCGGTGTTG+GGG | 0.521995 | 2.1:-36609485 | None:intergenic |
| ATGACGAGAGGTGTCATTTG+AGG | 0.522794 | 2.1:-36608573 | None:intergenic |
| GGATTTGGTGCTAAGTGGCT+TGG | 0.523784 | 2.1:+36610511 | MS.gene038844:CDS |
| CTGCAGGATTTGGTGCTAAG+TGG | 0.526710 | 2.1:+36610506 | MS.gene038844:CDS |
| GAAAGAAAAGCTTGTATCGA+TGG | 0.527863 | 2.1:+36619912 | MS.gene038844:CDS |
| AATCACTTTGCAGAATATGC+TGG | 0.528361 | 2.1:+36618138 | MS.gene038844:CDS |
| CAGATATCATTCCATAATTG+GGG | 0.535171 | 2.1:-36611695 | None:intergenic |
| CGAGAGGTGTCATTTGAGGA+AGG | 0.544148 | 2.1:-36608569 | None:intergenic |
| ACTACAGAGGCTATGACATA+GGG | 0.549695 | 2.1:+36618115 | MS.gene038844:CDS |
| ATAATGATTAATCACGAAGA+AGG | 0.551700 | 2.1:+36617285 | MS.gene038844:CDS |
| ACAGCCAGACAGACCTCAAG+AGG | 0.575109 | 2.1:+36618341 | MS.gene038844:CDS |
| AATTGGGGTTCCTTAGAACC+AGG | 0.577375 | 2.1:-36611680 | None:intergenic |
| TGAATGCAGTAATTTGTGCA+AGG | 0.578273 | 2.1:+36609083 | MS.gene038844:intron |
| TGAGGTAAATACATATGCAC+TGG | 0.582931 | 2.1:+36619693 | MS.gene038844:CDS |
| ACTGTGATTATTACCTCTTG+AGG | 0.585155 | 2.1:-36618354 | None:intergenic |
| CTCATTCCTTTCTAAAAGGT+AGG | 0.596387 | 2.1:-36619941 | None:intergenic |
| AAGATAATCAAAATCTATTG+GGG | 0.603378 | 2.1:-36619851 | None:intergenic |
| AAAGCGATCAATGATATGCT+CGG | 0.607018 | 2.1:-36609493 | None:intergenic |
| GAGAGGTGTCATTTGAGGAA+GGG | 0.608013 | 2.1:-36608568 | None:intergenic |
| ATTTGAGGAAGGGAAAGAGA+AGG | 0.609170 | 2.1:-36608558 | None:intergenic |
| GAAGGTTTCGGTTAAACAAG+GGG | 0.617236 | 2.1:+36609425 | MS.gene038844:CDS |
| AAAGTTGAGAAAATTTCTGG+AGG | 0.619890 | 2.1:+36609147 | MS.gene038844:CDS |
| GCAAGGACATGTTCAAGACC+TGG | 0.621936 | 2.1:+36609100 | MS.gene038844:CDS |
| GTTGGGGCCATACAATCTGA+TGG | 0.622325 | 2.1:-36609469 | None:intergenic |
| AGAATCACCTAAACTTGACC+AGG | 0.623881 | 2.1:-36609118 | None:intergenic |
| AATATAATACTAATAATGGG+TGG | 0.636817 | 2.1:+36608444 | None:intergenic |
| TGAAGGTTTCGGTTAAACAA+GGG | 0.654561 | 2.1:+36609424 | MS.gene038844:CDS |
| AGAGTTACTGACATGACGAG+AGG | 0.684298 | 2.1:-36608585 | None:intergenic |
| CAGAGACCAGAAAAGGTGTG+AGG | 0.696052 | 2.1:-36619716 | None:intergenic |
| TATGCATCATACAACTACAG+AGG | 0.713439 | 2.1:+36618102 | MS.gene038844:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 36608459 | 36619966 | 36608459 | ID=MS.gene038844 |
| chr2.1 | mRNA | 36608459 | 36619966 | 36608459 | ID=MS.gene038844.t1;Parent=MS.gene038844 |
| chr2.1 | exon | 36608459 | 36608595 | 36608459 | ID=MS.gene038844.t1.exon1;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36608459 | 36608595 | 36608459 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36609092 | 36609183 | 36609092 | ID=MS.gene038844.t1.exon2;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36609092 | 36609183 | 36609092 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36609421 | 36609527 | 36609421 | ID=MS.gene038844.t1.exon3;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36609421 | 36609527 | 36609421 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36610487 | 36610592 | 36610487 | ID=MS.gene038844.t1.exon4;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36610487 | 36610592 | 36610487 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36611612 | 36611734 | 36611612 | ID=MS.gene038844.t1.exon5;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36611612 | 36611734 | 36611612 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36613041 | 36613132 | 36613041 | ID=MS.gene038844.t1.exon6;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36613041 | 36613132 | 36613041 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36617222 | 36617306 | 36617222 | ID=MS.gene038844.t1.exon7;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36617222 | 36617306 | 36617222 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36618076 | 36618181 | 36618076 | ID=MS.gene038844.t1.exon8;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36618076 | 36618181 | 36618076 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36618296 | 36618362 | 36618296 | ID=MS.gene038844.t1.exon9;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36618296 | 36618362 | 36618296 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36619661 | 36619753 | 36619661 | ID=MS.gene038844.t1.exon10;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36619661 | 36619753 | 36619661 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| chr2.1 | exon | 36619841 | 36619966 | 36619841 | ID=MS.gene038844.t1.exon11;Parent=MS.gene038844.t1 |
| chr2.1 | CDS | 36619841 | 36619966 | 36619841 | ID=cds.MS.gene038844.t1;Parent=MS.gene038844.t1 |
| Gene Sequence |
| Protein sequence |
