AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene043476

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043476.t1 MTR_2g090910 100.000 223 0 0 1 223 1 223 5.62e-168 461
MS.gene043476.t1 MTR_3g094230 98.649 222 3 0 1 222 1 222 1.29e-165 456
MS.gene043476.t1 MTR_3g062600 93.119 218 15 0 1 218 1 218 3.61e-152 421
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene043476.t1 AT1G26910 88.991 218 24 0 1 218 1 218 1.29e-146 407
MS.gene043476.t1 AT1G14320 88.532 218 25 0 1 218 1 218 2.07e-145 404
MS.gene043476.t1 AT1G66580 88.128 219 26 0 1 219 1 219 7.25e-145 403
MS.gene043476.t1 AT1G26910 87.845 181 22 0 38 218 1 181 9.30e-118 333
MS.gene043476.t1 AT1G14320 76.596 94 20 1 127 218 31 124 1.15e-45 148
MS.gene043476.t1 AT1G14320 97.436 39 1 0 38 76 1 39 1.91e-20 83.6

Find 65 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACGACAGAATCGTGATTT+TGG 0.179385 2.2:-8528952 None:intergenic
ACATCGTAAATCCTGATCTT+AGG 0.319740 2.2:-8528985 None:intergenic
CTTTGACATGTACTTGTTGC+AGG 0.330350 2.2:-8529112 None:intergenic
GTTTCCTTTCTGTGTTCATC+TGG 0.355959 2.2:+8529035 MS.gene043476:CDS
CTGTGTTCATCTGGTCAGTT+GGG 0.370061 2.2:+8529044 MS.gene043476:CDS
ACTGGTATGAGAGGTGCTTT+TGG 0.372976 2.2:+8529237 MS.gene043476:CDS
ATGCATTCCACTTGAGAGTC+AGG 0.376262 2.2:+8529148 MS.gene043476:CDS
GTTTGTGCTAGAGTGGCTAT+TGG 0.382737 2.2:+8529273 MS.gene043476:CDS
TTTCATGTGCTGGTGCTGAT+AGG 0.390293 2.2:+8529208 MS.gene043476:CDS
TCTGTGTTCATCTGGTCAGT+TGG 0.393251 2.2:+8529043 MS.gene043476:CDS
ACCTTAGCATTCACCCCATC+GGG 0.415318 2.2:-8529599 None:intergenic
TTTGTGCTAGAGTGGCTATT+GGG 0.420431 2.2:+8529274 MS.gene043476:CDS
TTGCAGCTTCTTGGATGCCA+TGG 0.430564 2.2:+8529846 MS.gene043476:intron
CGATGTTGGCATGAAGAAGA+AGG 0.456800 2.2:+8529002 MS.gene043476:CDS
TGAGTATCTGAAGTTGAAAT+CGG 0.458837 2.2:+8529562 MS.gene043476:CDS
CATGTCAAAGTTTGCAGGAA+AGG 0.460338 2.2:+8529125 MS.gene043476:CDS
ATGGCTTGTTCTTAATTTGA+CGG 0.461186 2.2:-8528927 None:intergenic
TGCTAGAGTGGCTATTGGGC+AGG 0.472691 2.2:+8529278 MS.gene043476:CDS
AAGTACATGTCAAAGTTTGC+AGG 0.473692 2.2:+8529120 MS.gene043476:CDS
TCTTGGATGCCATGGACCAT+TGG 0.486612 2.2:+8529854 MS.gene043476:CDS
CGTGCCAAGTTTAAGTTCCC+TGG 0.487787 2.2:+8529360 MS.gene043476:CDS
TAAATCCTGATCTTAGGATC+AGG 0.495721 2.2:-8528979 None:intergenic
TACCTTAGCATTCACCCCAT+CGG 0.499742 2.2:-8529600 None:intergenic
TGCCAGCTGTAATGAGGCTT+AGG 0.501937 2.2:+8529908 MS.gene043476:CDS
TTTGAATGCCAGCTGTAATG+AGG 0.503491 2.2:+8529902 MS.gene043476:CDS
TTAGGTCTTACTTCTTCCCA+TGG 0.509780 2.2:-8527164 None:intergenic
TCTTGTTGATCCTGAGTACA+TGG 0.512666 2.2:-8529182 None:intergenic
AACAAGATGCTTTCATGTGC+TGG 0.512686 2.2:+8529198 MS.gene043476:CDS
CCATTGGCTAACCGTCAACC+TGG 0.512794 2.2:+8529870 MS.gene043476:CDS
CAACAACAGCCACCATGCTC+AGG 0.521206 2.2:+8529326 MS.gene043476:CDS
GCTCCTTTCTGTCCGTTGCA+AGG 0.530827 2.2:+8529302 MS.gene043476:CDS
ACGGCCAGGGAACTTAAACT+TGG 0.532430 2.2:-8529364 None:intergenic
AGATCATCGTTAGCAGGAAG+TGG 0.534059 2.2:+8529391 MS.gene043476:CDS
AGCCTAAGCCTCATTACAGC+TGG 0.535691 2.2:-8529910 None:intergenic
GTCAAAAGATCATCGTTAGC+AGG 0.537568 2.2:+8529385 MS.gene043476:CDS
TGTCCTTGCAACGGACAGAA+AGG 0.541207 2.2:-8529305 None:intergenic
GAGTTCCTGATCCTAAGATC+AGG 0.544600 2.2:+8528974 MS.gene043476:CDS
CCAGGTTGACGGTTAGCCAA+TGG 0.544617 2.2:-8529870 None:intergenic
TCAGGGTGTTTGTGCTAGAG+TGG 0.548804 2.2:+8529266 MS.gene043476:CDS
AAAACAGAATTATGCCCGAT+GGG 0.552482 2.2:+8529585 MS.gene043476:CDS
GACGGAGAGCCTCCTGAGCA+TGG 0.554611 2.2:-8529338 None:intergenic
AAGATCAGGATTTACGATGT+TGG 0.557539 2.2:+8528988 MS.gene043476:CDS
GACGGTTAGCCAATGGTCCA+TGG 0.564761 2.2:-8529863 None:intergenic
GGCTGTTGTTGTCCTTGCAA+CGG 0.568368 2.2:-8529314 None:intergenic
GGTGCTGATAGGCTTCAAAC+TGG 0.570540 2.2:+8529219 MS.gene043476:CDS
TCATCTGGTCAGTTGGGAGA+AGG 0.572889 2.2:+8529050 MS.gene043476:CDS
CTGACCAGATGAACACAGAA+AGG 0.573863 2.2:-8529039 None:intergenic
ATTTGACGGTAACACCTAGC+AGG 0.579048 2.2:-8528913 None:intergenic
TCAACTTCAGATACTCAGCT+CGG 0.581935 2.2:-8529556 None:intergenic
GCCCGATGGGGTGAATGCTA+AGG 0.582072 2.2:+8529598 MS.gene043476:CDS
TTCATCCATTCCATGTACTC+AGG 0.583517 2.2:+8529172 MS.gene043476:CDS
TGCATTCCACTTGAGAGTCA+GGG 0.588140 2.2:+8529149 MS.gene043476:CDS
CAACAGCCACCATGCTCAGG+AGG 0.593303 2.2:+8529329 MS.gene043476:CDS
GTGAGGCACTTGAAGCTGCT+AGG 0.594487 2.2:+8529085 MS.gene043476:CDS
GATGAACCCTGACTCTCAAG+TGG 0.599276 2.2:-8529155 None:intergenic
AGGCTTCAAACTGGTATGAG+AGG 0.600097 2.2:+8529228 MS.gene043476:CDS
CCAAAATCACGATTCTGTCG+TGG 0.626637 2.2:+8528952 MS.gene043476:CDS
GAAAACAGAATTATGCCCGA+TGG 0.626773 2.2:+8529584 MS.gene043476:CDS
TTGATCCTGAGTACATGGAA+TGG 0.632244 2.2:-8529177 None:intergenic
GATGTTGGCATGAAGAAGAA+GGG 0.638141 2.2:+8529003 MS.gene043476:CDS
GGAACTTAAACTTGGCACGA+CGG 0.658128 2.2:-8529356 None:intergenic
GAAGGAAAATGTCTCAAGTG+AGG 0.670840 2.2:+8529068 MS.gene043476:CDS
AAACAGAATTATGCCCGATG+GGG 0.689778 2.2:+8529586 MS.gene043476:CDS
GGAGAGCCTCCTGAGCATGG+TGG 0.689789 2.2:-8529335 None:intergenic
ACTCTAGCACAAACACCCTG+AGG 0.744760 2.2:-8529264 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAACAAATAATATATTAA+GGG - chr2.2:8527643-8527662 None:intergenic 10.0%
!! ATTTCATAAAAATTAGTACT+TGG - chr2.2:8528565-8528584 None:intergenic 15.0%
!! CTGTAACAAATAATATATTA+AGG - chr2.2:8527644-8527663 None:intergenic 15.0%
!! TTAAATTAATTGAGAATTTG+AGG + chr2.2:8527515-8527534 MS.gene043476:intron 15.0%
!! TTACATTCAAAATATAAACA+GGG - chr2.2:8528308-8528327 None:intergenic 15.0%
!! TTTCATAAAAATTAGTACTT+GGG - chr2.2:8528564-8528583 None:intergenic 15.0%
!!! ATCATGTTTGTTTTATTTAT+AGG + chr2.2:8528889-8528908 MS.gene043476:intron 15.0%
!!! GATTTGTGATTTAAAATTTA+GGG + chr2.2:8527395-8527414 MS.gene043476:intron 15.0%
!!! TCATGTTTGTTTTATTTATA+GGG + chr2.2:8528890-8528909 MS.gene043476:intron 15.0%
!!! TGATTTGTGATTTAAAATTT+AGG + chr2.2:8527394-8527413 MS.gene043476:intron 15.0%
!! AAAGGTTTACTAAAACATAA+AGG - chr2.2:8527803-8527822 None:intergenic 20.0%
!! AATGATGATATTTATCCATA+CGG + chr2.2:8527999-8528018 MS.gene043476:intron 20.0%
!! AGAAATGAATGAAAATTAGA+AGG - chr2.2:8527193-8527212 None:intergenic 20.0%
!! ATTGAAATAAAACGAATTCA+TGG - chr2.2:8527265-8527284 None:intergenic 20.0%
!! CAATAATTAGAGTTAATACA+TGG - chr2.2:8529497-8529516 None:intergenic 20.0%
!! CTATAATTAGAATTAGTACT+TGG - chr2.2:8528331-8528350 None:intergenic 20.0%
!! GAAATGAATGAAAATTAGAA+GGG - chr2.2:8527192-8527211 None:intergenic 20.0%
!! GTTACATTCAAAATATAAAC+AGG - chr2.2:8528309-8528328 None:intergenic 20.0%
!!! AAAAGAACTTATAAGTTGAA+AGG - chr2.2:8529820-8529839 None:intergenic 20.0%
!!! ACCTTTTTGATTTTTTATTG+CGG + chr2.2:8527817-8527836 MS.gene043476:intron 20.0%
!!! AGATATGATGATGTTTATAT+TGG + chr2.2:8527293-8527312 MS.gene043476:intron 20.0%
!!! ATGTTAGTTTGGAATATATT+AGG + chr2.2:8529712-8529731 MS.gene043476:intron 20.0%
!!! CTAATTATTGTTGTTTGTAA+TGG + chr2.2:8529507-8529526 MS.gene043476:intron 20.0%
!!! TGAATGTCATGATATTTTAA+TGG + chr2.2:8528797-8528816 MS.gene043476:intron 20.0%
!!! TGTTAGTTTGGAATATATTA+GGG + chr2.2:8529713-8529732 MS.gene043476:intron 20.0%
! AAAAATAAGCTAATCCAAAC+GGG - chr2.2:8527578-8527597 None:intergenic 25.0%
! AAATGAAATGAAGAAACAAC+TGG - chr2.2:8527242-8527261 None:intergenic 25.0%
! ACAGTATAATCAGAATAGTA+AGG - chr2.2:8528429-8528448 None:intergenic 25.0%
! ACCGCAATAAAAAATCAAAA+AGG - chr2.2:8527821-8527840 None:intergenic 25.0%
! ATGAAAATTAGAAGGGAATT+AGG - chr2.2:8527185-8527204 None:intergenic 25.0%
! ATGATTATCCTATTATTGTG+TGG + chr2.2:8527695-8527714 MS.gene043476:intron 25.0%
! CAAAAATAAGCTAATCCAAA+CGG - chr2.2:8527579-8527598 None:intergenic 25.0%
! GAACTAACAAACAATTACAA+TGG - chr2.2:8527721-8527740 None:intergenic 25.0%
! TAACTTGAATCATGTTAGTT+TGG + chr2.2:8529701-8529720 MS.gene043476:intron 25.0%
! TCCATTATGTATTATGTGTT+GGG - chr2.2:8527917-8527936 None:intergenic 25.0%
!! GTGATATTTCTGAATAATCA+CGG - chr2.2:8528680-8528699 None:intergenic 25.0%
!!! TGTTTGTTATTGATGAATTC+AGG + chr2.2:8527758-8527777 MS.gene043476:intron 25.0%
AACAGGGAATTAGTAACAAT+AGG - chr2.2:8528292-8528311 None:intergenic 30.0%
AATCTGTTTGAACACTCAAA+TGG + chr2.2:8529758-8529777 MS.gene043476:intron 30.0%
ACAAATCTACAATCGAAGTA+AGG - chr2.2:8529442-8529461 None:intergenic 30.0%
ACGGTATAATCAGAATAGTA+AGG - chr2.2:8528661-8528680 None:intergenic 30.0%
CTCCATTATGTATTATGTGT+TGG - chr2.2:8527918-8527937 None:intergenic 30.0%
TAATATATTAAGGGTCTGTC+TGG - chr2.2:8527634-8527653 None:intergenic 30.0%
TAGTAAGATCAAATTGAGCA+TGG - chr2.2:8527889-8527908 None:intergenic 30.0%
TCAGAAATATCACCATCTTT+CGG + chr2.2:8528687-8528706 MS.gene043476:intron 30.0%
TCCCAACACATAATACATAA+TGG + chr2.2:8527913-8527932 MS.gene043476:intron 30.0%
TTAGTACTTGGGTATATCTA+TGG - chr2.2:8528553-8528572 None:intergenic 30.0%
TTGTTTGTAATGGTTCTTCA+GGG + chr2.2:8529517-8529536 MS.gene043476:intron 30.0%
! ATATTTTCTTCCTCATGCTA+AGG + chr2.2:8529663-8529682 MS.gene043476:intron 30.0%
! CTATCATTCTTTGTTGTCTT+TGG + chr2.2:8527951-8527970 MS.gene043476:intron 30.0%
! TAGCTTGACATTTCGTTTTA+CGG + chr2.2:8528210-8528229 MS.gene043476:intron 30.0%
! TGAGTATCTGAAGTTGAAAT+CGG + chr2.2:8529562-8529581 MS.gene043476:CDS 30.0%
!! ATGGCTTGTTCTTAATTTGA+CGG - chr2.2:8528930-8528949 None:intergenic 30.0%
!!! TTGATTTTTTATTGCGGTAG+TGG + chr2.2:8527823-8527842 MS.gene043476:intron 30.0%
AAAACAGAATTATGCCCGAT+GGG + chr2.2:8529585-8529604 MS.gene043476:CDS 35.0%
AAGAGAGATCAGAAATTGGA+AGG - chr2.2:8527351-8527370 None:intergenic 35.0%
AAGATCAGGATTTACGATGT+TGG + chr2.2:8528988-8529007 MS.gene043476:CDS 35.0%
AAGTACATGTCAAAGTTTGC+AGG + chr2.2:8529120-8529139 MS.gene043476:CDS 35.0%
ACATCGTAAATCCTGATCTT+AGG - chr2.2:8528988-8529007 None:intergenic 35.0%
AGAGAGATCAGAAATTGGAA+GGG - chr2.2:8527350-8527369 None:intergenic 35.0%
CGATTGTAGATTTGTAGTCT+GGG + chr2.2:8529447-8529466 MS.gene043476:intron 35.0%
CTGAAAGAGAGATCAGAAAT+TGG - chr2.2:8527355-8527374 None:intergenic 35.0%
GTTGTTTGTAATGGTTCTTC+AGG + chr2.2:8529516-8529535 MS.gene043476:intron 35.0%
TAAATCCTGATCTTAGGATC+AGG - chr2.2:8528982-8529001 None:intergenic 35.0%
TACAATGGCCACACAATAAT+AGG - chr2.2:8527706-8527725 None:intergenic 35.0%
TCATCAAAAACAGAACCGTA+TGG - chr2.2:8528017-8528036 None:intergenic 35.0%
TCCAAAACATCACCATCTTT+CGG + chr2.2:8528455-8528474 MS.gene043476:intron 35.0%
TCGATTGTAGATTTGTAGTC+TGG + chr2.2:8529446-8529465 MS.gene043476:intron 35.0%
TGTTTGTAATGGTTCTTCAG+GGG + chr2.2:8529518-8529537 MS.gene043476:intron 35.0%
TTCTAGACATCAGATACCAT+AGG - chr2.2:8528173-8528192 None:intergenic 35.0%
! AGCTTGACATTTCGTTTTAC+GGG + chr2.2:8528211-8528230 MS.gene043476:intron 35.0%
! CAGAATCGTGATTTTGGATA+TGG - chr2.2:8528949-8528968 None:intergenic 35.0%
! GGGATAGATCAAACTTTTAC+AGG + chr2.2:8528232-8528251 MS.gene043476:intron 35.0%
! TGCTAACGATGATCTTTTGA+CGG - chr2.2:8529386-8529405 None:intergenic 35.0%
! TTTGTGATTTTGCAGCTTCT+TGG + chr2.2:8529837-8529856 MS.gene043476:intron 35.0%
!! TTTTATTTATAGGGCCTGCT+AGG + chr2.2:8528899-8528918 MS.gene043476:intron 35.0%
!!! TGTTCGTCAACTTTTTTACC+AGG + chr2.2:8527854-8527873 MS.gene043476:intron 35.0%
AAACAGAATTATGCCCGATG+GGG + chr2.2:8529586-8529605 MS.gene043476:CDS 40.0%
AACAAGATGCTTTCATGTGC+TGG + chr2.2:8529198-8529217 MS.gene043476:CDS 40.0%
AAGTACATGTCCTTAGCATG+AGG - chr2.2:8529676-8529695 None:intergenic 40.0%
CATGTCAAAGTTTGCAGGAA+AGG + chr2.2:8529125-8529144 MS.gene043476:CDS 40.0%
GAAAACAGAATTATGCCCGA+TGG + chr2.2:8529584-8529603 MS.gene043476:CDS 40.0%
GAAGGAAAATGTCTCAAGTG+AGG + chr2.2:8529068-8529087 MS.gene043476:CDS 40.0%
GAGAGATCAGAAATTGGAAG+GGG - chr2.2:8527349-8527368 None:intergenic 40.0%
GATGTTGGCATGAAGAAGAA+GGG + chr2.2:8529003-8529022 MS.gene043476:CDS 40.0%
GCATTCAAAAAAGCTCTTCC+AGG - chr2.2:8529891-8529910 None:intergenic 40.0%
GTCAAAAGATCATCGTTAGC+AGG + chr2.2:8529385-8529404 MS.gene043476:CDS 40.0%
GTCACGGTGAATAAGAAACA+CGG - chr2.2:8527320-8527339 None:intergenic 40.0%
GTTTCCTTTCTGTGTTCATC+TGG + chr2.2:8529035-8529054 MS.gene043476:CDS 40.0%
TATTAAGGGTCTGTCTGGAT+TGG - chr2.2:8527629-8527648 None:intergenic 40.0%
TCAACTTCAGATACTCAGCT+CGG - chr2.2:8529559-8529578 None:intergenic 40.0%
TCTTGTTGATCCTGAGTACA+TGG - chr2.2:8529185-8529204 None:intergenic 40.0%
TGCGTCATGTATATTGCCTA+TGG + chr2.2:8528154-8528173 MS.gene043476:intron 40.0%
TTCATCCATTCCATGTACTC+AGG + chr2.2:8529172-8529191 MS.gene043476:CDS 40.0%
TTGATCCTGAGTACATGGAA+TGG - chr2.2:8529180-8529199 None:intergenic 40.0%
TTTGAATGCCAGCTGTAATG+AGG + chr2.2:8529902-8529921 MS.gene043476:CDS 40.0%
TTTGTGCTAGAGTGGCTATT+GGG + chr2.2:8529274-8529293 MS.gene043476:CDS 40.0%
! AAAAAGCTCTTCCAGGTTGA+CGG - chr2.2:8529884-8529903 None:intergenic 40.0%
! ATAGCTTGCAAGAGATCAGA+TGG - chr2.2:8528058-8528077 None:intergenic 40.0%
! CTTTGACATGTACTTGTTGC+AGG - chr2.2:8529115-8529134 None:intergenic 40.0%
! GCTTGACATTTCGTTTTACG+GGG + chr2.2:8528212-8528231 MS.gene043476:intron 40.0%
! TTTGTAGTCTGGGATTACTC+TGG + chr2.2:8529457-8529476 MS.gene043476:intron 40.0%
! TTTTGAATCAAGTGCCCGTT+TGG + chr2.2:8527561-8527580 MS.gene043476:intron 40.0%
AGATCATCGTTAGCAGGAAG+TGG + chr2.2:8529391-8529410 MS.gene043476:CDS 45.0%
AGGCTTCAAACTGGTATGAG+AGG + chr2.2:8529228-8529247 MS.gene043476:CDS 45.0%
ATGCAATCTCAGCCGAAAGA+TGG - chr2.2:8528702-8528721 None:intergenic 45.0%
ATGCATTCCACTTGAGAGTC+AGG + chr2.2:8529148-8529167 MS.gene043476:CDS 45.0%
ATTTGACGGTAACACCTAGC+AGG - chr2.2:8528916-8528935 None:intergenic 45.0%
CCAAAATCACGATTCTGTCG+TGG + chr2.2:8528952-8528971 MS.gene043476:CDS 45.0%
CGATGTTGGCATGAAGAAGA+AGG + chr2.2:8529002-8529021 MS.gene043476:CDS 45.0%
CTGACCAGATGAACACAGAA+AGG - chr2.2:8529042-8529061 None:intergenic 45.0%
CTGTGTTCATCTGGTCAGTT+GGG + chr2.2:8529044-8529063 MS.gene043476:CDS 45.0%
GAGTTCCTGATCCTAAGATC+AGG + chr2.2:8528974-8528993 MS.gene043476:CDS 45.0%
GGAACTTAAACTTGGCACGA+CGG - chr2.2:8529359-8529378 None:intergenic 45.0%
GTTTGTGCTAGAGTGGCTAT+TGG + chr2.2:8529273-8529292 MS.gene043476:CDS 45.0%
TACCTTAGCATTCACCCCAT+CGG - chr2.2:8529603-8529622 None:intergenic 45.0%
TCTGTGTTCATCTGGTCAGT+TGG + chr2.2:8529043-8529062 MS.gene043476:CDS 45.0%
TGCATTCCACTTGAGAGTCA+GGG + chr2.2:8529149-8529168 MS.gene043476:CDS 45.0%
! CCACGACAGAATCGTGATTT+TGG - chr2.2:8528955-8528974 None:intergenic 45.0%
! TTTTACAGGTCTTGCGACAC+AGG + chr2.2:8528246-8528265 MS.gene043476:intron 45.0%
!! GCCGAAAGATGGTGATGTTT+TGG - chr2.2:8528459-8528478 None:intergenic 45.0%
!! TTTCATGTGCTGGTGCTGAT+AGG + chr2.2:8529208-8529227 MS.gene043476:CDS 45.0%
!!! ACTGGTATGAGAGGTGCTTT+TGG + chr2.2:8529237-8529256 MS.gene043476:CDS 45.0%
ACCTTAGCATTCACCCCATC+GGG - chr2.2:8529602-8529621 None:intergenic 50.0%
ACGGCCAGGGAACTTAAACT+TGG - chr2.2:8529367-8529386 None:intergenic 50.0%
ACTCTAGCACAAACACCCTG+AGG - chr2.2:8529267-8529286 None:intergenic 50.0%
CTGCAATCTCAGCCGAAAGA+TGG - chr2.2:8528470-8528489 None:intergenic 50.0%
GATGAACCCTGACTCTCAAG+TGG - chr2.2:8529158-8529177 None:intergenic 50.0%
GGCTGTTGTTGTCCTTGCAA+CGG - chr2.2:8529317-8529336 None:intergenic 50.0%
TCATCTGGTCAGTTGGGAGA+AGG + chr2.2:8529050-8529069 MS.gene043476:CDS 50.0%
TCTTGGATGCCATGGACCAT+TGG + chr2.2:8529854-8529873 MS.gene043476:CDS 50.0%
TGTCCTTGCAACGGACAGAA+AGG - chr2.2:8529308-8529327 None:intergenic 50.0%
TTGCAGCTTCTTGGATGCCA+TGG + chr2.2:8529846-8529865 MS.gene043476:intron 50.0%
TTGGAAGGGGTTGTCTGTCA+CGG - chr2.2:8527336-8527355 None:intergenic 50.0%
! ACGATGATCTTTTGACGGCC+AGG - chr2.2:8529381-8529400 None:intergenic 50.0%
! AGGTGCTTTTGGTAAGCCTC+AGG + chr2.2:8529248-8529267 MS.gene043476:CDS 50.0%
! CGATGATCTTTTGACGGCCA+GGG - chr2.2:8529380-8529399 None:intergenic 50.0%
! CGTGCCAAGTTTAAGTTCCC+TGG + chr2.2:8529360-8529379 MS.gene043476:CDS 50.0%
! GGTGCTTTTGGTAAGCCTCA+GGG + chr2.2:8529249-8529268 MS.gene043476:CDS 50.0%
! TCAGGGTGTTTGTGCTAGAG+TGG + chr2.2:8529266-8529285 MS.gene043476:CDS 50.0%
!! GGTGCTGATAGGCTTCAAAC+TGG + chr2.2:8529219-8529238 MS.gene043476:CDS 50.0%
CAACAACAGCCACCATGCTC+AGG + chr2.2:8529326-8529345 MS.gene043476:CDS 55.0%
GCTCCTTTCTGTCCGTTGCA+AGG + chr2.2:8529302-8529321 MS.gene043476:CDS 55.0%
TGAGCATGGCAAACAGTGCC+TGG - chr2.2:8527875-8527894 None:intergenic 55.0%
TGCTAGAGTGGCTATTGGGC+AGG + chr2.2:8529278-8529297 MS.gene043476:CDS 55.0%
! CCAGGTTGACGGTTAGCCAA+TGG - chr2.2:8529873-8529892 None:intergenic 55.0%
! CCATTGGCTAACCGTCAACC+TGG + chr2.2:8529870-8529889 MS.gene043476:CDS 55.0%
! GACGGTTAGCCAATGGTCCA+TGG - chr2.2:8529866-8529885 None:intergenic 55.0%
! GTGAGGCACTTGAAGCTGCT+AGG + chr2.2:8529085-8529104 MS.gene043476:CDS 55.0%
CAACAGCCACCATGCTCAGG+AGG + chr2.2:8529329-8529348 MS.gene043476:CDS 60.0%
GCCCGATGGGGTGAATGCTA+AGG + chr2.2:8529598-8529617 MS.gene043476:CDS 60.0%
GACGGAGAGCCTCCTGAGCA+TGG - chr2.2:8529341-8529360 None:intergenic 65.0%
GGAGAGCCTCCTGAGCATGG+TGG - chr2.2:8529338-8529357 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 8527166 8529929 8527166 ID=MS.gene043476
chr2.2 mRNA 8527166 8529929 8527166 ID=MS.gene043476.t1;Parent=MS.gene043476
chr2.2 exon 8527166 8527175 8527166 ID=MS.gene043476.t1.exon1;Parent=MS.gene043476.t1
chr2.2 CDS 8527166 8527175 8527166 ID=cds.MS.gene043476.t1;Parent=MS.gene043476.t1
chr2.2 exon 8528911 8529412 8528911 ID=MS.gene043476.t1.exon2;Parent=MS.gene043476.t1
chr2.2 CDS 8528911 8529412 8528911 ID=cds.MS.gene043476.t1;Parent=MS.gene043476.t1
chr2.2 exon 8529538 8529619 8529538 ID=MS.gene043476.t1.exon3;Parent=MS.gene043476.t1
chr2.2 CDS 8529538 8529619 8529538 ID=cds.MS.gene043476.t1;Parent=MS.gene043476.t1
chr2.2 exon 8529852 8529929 8529852 ID=MS.gene043476.t1.exon4;Parent=MS.gene043476.t1
chr2.2 CDS 8529852 8529929 8529852 ID=cds.MS.gene043476.t1;Parent=MS.gene043476.t1
Gene Sequence

>MS.gene043476

ATGGGAAGAAGTAAGACCTAATTCCCTTCTAATTTTCATTCATTTCTCTGTGTTTGTTTCGTTTCTGATTATTCCAGTTGTTTCTTCATTTCATTTCCATGAATTCGTTTTATTTCAATTTTTAAAGAGATATGATGATGTTTATATTGGTCCGTGTTTCTTATTCACCGTGACAGACAACCCCTTCCAATTTCTGATCTCTCTTTCAGACATTTTTTTTTAAAATTTTGATTTGTGATTTAAAATTTAGGGTTTATAAATCATTATATATACGAAATCTATTGCAGATTTATTTTATGTGTTGATTTGATGCTGCTGTAAGTTGAATCTGAATAATGTTTTAGCTGTTTTAAATTAATTGAGAATTTGAGGAGTTTATTAGAATGAATGATTTGTTTTGAATCAAGTGCCCGTTTGGATTAGCTTATTTTTGAGCTTAGTCAGAAATAATAATTATAAACCAATCCAGACAGACCCTTAATATATTATTTGTTACAGATATGAAATTTGTTTTATGCTTTTGAATTTCATGATTATCCTATTATTGTGTGGCCATTGTAATTGTTTGTTAGTTCTTGTGCATGAACTGTATTGTTTGTTATTGATGAATTCAGGATCTTAAGTTCTTTTCAAACCTTTATGTTTTAGTAAACCTTTTTGATTTTTTATTGCGGTAGTGGCTGCTTTTTGTTCGTCAACTTTTTTACCAGGCACTGTTTGCCATGCTCAATTTGATCTTACTAGTATTCCCAACACATAATACATAATGGAGTCTATTTTAATGTCTATCATTCTTTGTTGTCTTTGGTACTACTATTATGAAATGCAAGAGAAATGATGATATTTATCCATACGGTTCTGTTTTTGATGATGAAACAAACATGCACTACCATCTGATCTCTTGCAAGCTATAATATTTTCTAAATTTTAATTCTATAATTAAAAAAAATTAAGTCTCTTATATTGTATTATTTGTTTTCATTGTTTATGCGTCATGTATATTGCCTATGGTATCTGATGTCTAGAATTCTGACTTTTGTCTAGTAGCTTGACATTTCGTTTTACGGGGATAGATCAAACTTTTACAGGTCTTGCGACACAGGAGTTAACTTAAATATAAACACCTATTGTTACTAATTCCCTGTTTATATTTTGAATGTAACCAAGTACTAATTCTAATTATAGTTATTCAAATCGTTATATTTTTTTTTTGTTTTGTTATTGTTTATTGTTCTGCAGTCTATGATGCAATTTTTATATCCTTACTATTCTGATTATACTGTGATTATTCCAAAACATCACCATCTTTCGGCTGAGATTGCAGTTCTATTTTTTTATTTACTTAATAATATTAATTATATCAATGTCGTTATTAAGCATAATGCCATAGATATACCCAAGTACTAATTTTTATGAAATTTATTCAAATGATTATATGTTTTTTGTTTTGTTATTGTGTATTGTTCTGCAATCTATGATGCAATTTTTATATCCTTACTATTCTGATTATACCGTGATTATTCAGAAATATCACCATCTTTCGGCTGAGATTGCATTTCTAAATTTTTTTTCACTTATTAATATTAATTAGATGTTAATCTTTATTAAGCATGAAACATAACAAAAATCTTTGAATGTCATGATATTTTAATGGATGAAACTGATGCATAGTGCATTTTTGTATCTTGATCATATGATGTATTGTACTATTTATGTGATTGTAATCATGTTTGTTTTATTTATAGGGCCTGCTAGGTGTTACCGTCAAATTAAGAACAAGCCATATCCAAAATCACGATTCTGTCGTGGAGTTCCTGATCCTAAGATCAGGATTTACGATGTTGGCATGAAGAAGAAGGGTGTTGATGAGTTTCCTTTCTGTGTTCATCTGGTCAGTTGGGAGAAGGAAAATGTCTCAAGTGAGGCACTTGAAGCTGCTAGGATTGCCTGCAACAAGTACATGTCAAAGTTTGCAGGAAAGGATGCATTCCACTTGAGAGTCAGGGTTCATCCATTCCATGTACTCAGGATCAACAAGATGCTTTCATGTGCTGGTGCTGATAGGCTTCAAACTGGTATGAGAGGTGCTTTTGGTAAGCCTCAGGGTGTTTGTGCTAGAGTGGCTATTGGGCAGGTGCTCCTTTCTGTCCGTTGCAAGGACAACAACAGCCACCATGCTCAGGAGGCTCTCCGTCGTGCCAAGTTTAAGTTCCCTGGCCGTCAAAAGATCATCGTTAGCAGGAAGTGGTAAGTATTTGAAAAATGAAATATCACCTTACTTCGATTGTAGATTTGTAGTCTGGGATTACTCTGGCGTTTTACTTTAATCCATGTATTAACTCTAATTATTGTTGTTTGTAATGGTTCTTCAGGGGATTCACAAAGATCAACCGAGCTGAGTATCTGAAGTTGAAATCGGAAAACAGAATTATGCCCGATGGGGTGAATGCTAAGGTAAATGTGATGCTGCATGTGAATTCATTGTACTGCATGTTTCATATTTTCTTCCTCATGCTAAGGACATGTACTTTTTGCTAACTTGAATCATGTTAGTTTGGAATATATTAGGGCATGTGTCTGATTTGTGAATATAATCTGTTTGAACACTCAAATGGATACTTTTCTTCAGTTAGTTTTGTTGAAGTAATTAACCTTTCAACTTATAAGTTCTTTTGTGATTTTGCAGCTTCTTGGATGCCATGGACCATTGGCTAACCGTCAACCTGGAAGAGCTTTTTTGAATGCCAGCTGTAATGAGGCTTAG
Protein sequence

>MS.gene043476.t1

MGRRPARCYRQIKNKPYPKSRFCRGVPDPKIRIYDVGMKKKGVDEFPFCVHLVSWEKENVSSEALEAARIACNKYMSKFAGKDAFHLRVRVHPFHVLRINKMLSCAGADRLQTGMRGAFGKPQGVCARVAIGQVLLSVRCKDNNSHHAQEALRRAKFKFPGRQKIIVSRKWGFTKINRAEYLKLKSENRIMPDGVNAKLLGCHGPLANRQPGRAFLNASCNEA