Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044565.t1 | XP_024629315.1 | 82.6 | 470 | 74 | 4 | 1 | 470 | 1 | 462 | 3.00E-213 | 751.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044565.t1 | Q557M8 | 22.4 | 393 | 222 | 10 | 8 | 382 | 14 | 341 | 1.5e-11 | 72.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044565.t1 | A0A396IJ19 | 89.8 | 470 | 48 | 0 | 1 | 470 | 1 | 470 | 3.2e-212 | 747.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene044565 | MS.gene39443 | PPI |
| MS.gene007951 | MS.gene044565 | PPI |
| MS.gene007950 | MS.gene044565 | PPI |
| MS.gene31944 | MS.gene044565 | PPI |
| MS.gene031871 | MS.gene044565 | PPI |
| MS.gene052488 | MS.gene044565 | PPI |
| MS.gene33782 | MS.gene044565 | PPI |
| MS.gene08242 | MS.gene044565 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044565.t1 | MTR_8g077000 | 84.394 | 487 | 51 | 6 | 1 | 470 | 1 | 479 | 0.0 | 780 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene044565.t1 | AT1G03280 | 59.227 | 466 | 156 | 14 | 1 | 451 | 12 | 458 | 2.21e-178 | 508 |
| MS.gene044565.t1 | AT1G03280 | 59.227 | 466 | 156 | 14 | 1 | 451 | 12 | 458 | 2.21e-178 | 508 |
| MS.gene044565.t1 | AT1G03280 | 59.227 | 466 | 156 | 14 | 1 | 451 | 12 | 458 | 2.21e-178 | 508 |
| MS.gene044565.t1 | AT4G20340 | 52.174 | 460 | 192 | 12 | 1 | 451 | 13 | 453 | 1.85e-145 | 424 |
| MS.gene044565.t1 | AT4G20340 | 52.174 | 460 | 192 | 12 | 1 | 451 | 13 | 453 | 1.85e-145 | 424 |
| MS.gene044565.t1 | AT4G20810 | 47.548 | 469 | 185 | 16 | 8 | 469 | 1 | 415 | 1.79e-130 | 384 |
| MS.gene044565.t1 | AT4G20340 | 52.542 | 413 | 169 | 12 | 48 | 451 | 4 | 398 | 8.29e-128 | 377 |
Find 100 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTATCACTTCTTCCAGTTT+TGG | 0.162141 | 8.1:-14274763 | None:intergenic |
| AACTGACGGTACATCTTTAA+AGG | 0.182546 | 8.1:+14280293 | MS.gene044565:CDS |
| GACTTGCCTGTTCCAGAATT+TGG | 0.189652 | 8.1:+14278059 | MS.gene044565:CDS |
| ATCTGCTGCGTTCGCATCTT+GGG | 0.226916 | 8.1:-14280687 | None:intergenic |
| CATCTGCTGCGTTCGCATCT+TGG | 0.249410 | 8.1:-14280688 | None:intergenic |
| TCGTCCTCGTCGTCTTCTTC+TGG | 0.291126 | 8.1:-14282562 | None:intergenic |
| GGGCTGATTATCTCCTTTGC+TGG | 0.323516 | 8.1:-14274742 | None:intergenic |
| GCTTGAAGACTACCAAATTC+TGG | 0.326095 | 8.1:-14278071 | None:intergenic |
| AAATCTCATCTGGGATATAA+TGG | 0.332016 | 8.1:+14278155 | MS.gene044565:CDS |
| GAGAAATTGGTACTTGTTGT+AGG | 0.340168 | 8.1:-14274588 | None:intergenic |
| GGAGATAATCAGCCCAAAAC+TGG | 0.349455 | 8.1:+14274750 | MS.gene044565:CDS |
| ACGACGGCGTGAAAAGTTAA+AGG | 0.370019 | 8.1:+14276573 | MS.gene044565:CDS |
| TGGTGATTCTAAATCTCATC+TGG | 0.375711 | 8.1:+14278145 | MS.gene044565:CDS |
| TGGGAAGAGGCTCCTGTTAG+AGG | 0.378747 | 8.1:+14280785 | MS.gene044565:CDS |
| GAAGCCCGAGCTAGTGCAGC+TGG | 0.384987 | 8.1:+14278098 | MS.gene044565:CDS |
| AGAGAACAAGAACACCATTC+AGG | 0.401351 | 8.1:+14276257 | MS.gene044565:CDS |
| CACGCCCAGCTGCACTAGCT+CGG | 0.403153 | 8.1:-14278103 | None:intergenic |
| GCTTAAGAAGCAACGCGAAT+TGG | 0.412214 | 8.1:+14280646 | MS.gene044565:CDS |
| TTATTTCAGATACAATGCTT+TGG | 0.415674 | 8.1:+14276414 | MS.gene044565:intron |
| GGCTGAAGTGTACCTCTAAC+AGG | 0.418995 | 8.1:-14280797 | None:intergenic |
| GCAGCTGGGCGTGCAGCTAA+TGG | 0.419366 | 8.1:+14278113 | MS.gene044565:CDS |
| ATAAATTAGCTGCTCAAGAC+GGG | 0.426008 | 8.1:+14276515 | MS.gene044565:CDS |
| GTTGCTTTGTATGCAATTTG+AGG | 0.441479 | 8.1:-14274957 | None:intergenic |
| ATTTGGTAGTCTTCAAGCTT+GGG | 0.444477 | 8.1:+14278076 | MS.gene044565:CDS |
| ACGCCCAGCTGCACTAGCTC+GGG | 0.452951 | 8.1:-14278102 | None:intergenic |
| CGGTTGGAAGTACTGCTAGA+AGG | 0.463810 | 8.1:-14280713 | None:intergenic |
| AGGAAAAGAGGGAGAAGAAA+AGG | 0.464405 | 8.1:+14275953 | MS.gene044565:CDS |
| AGATTATCACTAGAGATCAT+AGG | 0.477747 | 8.1:+14275013 | MS.gene044565:CDS |
| AATTTGGTAGTCTTCAAGCT+TGG | 0.481156 | 8.1:+14278075 | MS.gene044565:CDS |
| TTCCACTGTGAACGTTGTAA+TGG | 0.482559 | 8.1:+14276472 | MS.gene044565:CDS |
| AGACTACCAAATTCTGGAAC+AGG | 0.482675 | 8.1:-14278065 | None:intergenic |
| AAAGGACATGCTTCAGAAGA+TGG | 0.491664 | 8.1:+14276591 | MS.gene044565:CDS |
| CCTCCATGGATGATTACATC+AGG | 0.493168 | 8.1:+14280321 | MS.gene044565:CDS |
| AAATGCAATTGGAGCTTACA+AGG | 0.494474 | 8.1:+14282510 | MS.gene044565:CDS |
| TGTACATATATATTCCTGAA+TGG | 0.494787 | 8.1:-14276271 | None:intergenic |
| TTGTTCTTGACGCGCTCACC+AGG | 0.495221 | 8.1:+14274802 | MS.gene044565:CDS |
| ACAGCGTGGAGAGGTGAAGC+AGG | 0.499690 | 8.1:+14280362 | MS.gene044565:CDS |
| TATGTACAAACTGTGGCAAA+AGG | 0.503511 | 8.1:+14276286 | MS.gene044565:CDS |
| TGAAGCTACGACAGGAAAAG+AGG | 0.510943 | 8.1:+14275941 | MS.gene044565:CDS |
| AGAAATTGGTACTTGTTGTA+GGG | 0.511459 | 8.1:-14274587 | None:intergenic |
| ACCTTGGTATCTCCGCTGTA+TGG | 0.515063 | 8.1:-14278188 | None:intergenic |
| GTCTTCTTCTGGCTGAGCAT+CGG | 0.526811 | 8.1:-14282551 | None:intergenic |
| GACGATATCGACTGGGAGGA+AGG | 0.528222 | 8.1:+14282580 | MS.gene044565:CDS |
| TCTGCTGCGTTCGCATCTTG+GGG | 0.534141 | 8.1:-14280686 | None:intergenic |
| CGACTGACTTGATCTACTAA+AGG | 0.542394 | 8.1:-14278029 | None:intergenic |
| TGATGGTACCGAGTGGGAAG+AGG | 0.544924 | 8.1:+14280772 | MS.gene044565:CDS |
| GATAAATTAGCTGCTCAAGA+CGG | 0.549791 | 8.1:+14276514 | MS.gene044565:CDS |
| ACGACGAGGACGATATCGAC+TGG | 0.550692 | 8.1:+14282572 | MS.gene044565:CDS |
| ATACCTGATGTAATCATCCA+TGG | 0.551552 | 8.1:-14280324 | None:intergenic |
| TTGATTTCAAGCCAACTTGG+CGG | 0.551942 | 8.1:-14280733 | None:intergenic |
| TTTCAAGCCAACTTGGCGGT+TGG | 0.553993 | 8.1:-14280729 | None:intergenic |
| TTTAGTAGATCAAGTCAGTC+GGG | 0.559290 | 8.1:+14278031 | MS.gene044565:CDS |
| CAGGTGGTAGTTGACTTCAA+TGG | 0.563361 | 8.1:+14280237 | MS.gene044565:intron |
| TTTATAGGATGAGTATGTCA+AGG | 0.564885 | 8.1:+14280607 | MS.gene044565:intron |
| TATGACGATCTTACCAGCAA+AGG | 0.565230 | 8.1:+14274729 | MS.gene044565:CDS |
| AGTACTTCCAACCGCCAAGT+TGG | 0.569471 | 8.1:+14280722 | MS.gene044565:CDS |
| TGCAGATATATGATGTGGTT+AGG | 0.570661 | 8.1:+14276193 | MS.gene044565:intron |
| AACAGGAGCCTCTTCCCACT+CGG | 0.576584 | 8.1:-14280780 | None:intergenic |
| GTATCTCCGCTGTATGGCAA+TGG | 0.577878 | 8.1:-14278182 | None:intergenic |
| AAGCCCGAGCTAGTGCAGCT+GGG | 0.582139 | 8.1:+14278099 | MS.gene044565:CDS |
| AAACGTGAGGAAGATGATGA+TGG | 0.590028 | 8.1:+14280755 | MS.gene044565:CDS |
| CTTTAGTAGATCAAGTCAGT+CGG | 0.591765 | 8.1:+14278030 | MS.gene044565:CDS |
| GTTGACTTCAATGGTACTGA+AGG | 0.599548 | 8.1:+14280246 | MS.gene044565:CDS |
| GGTACTGAAGGCAAAGGAGA+GGG | 0.602003 | 8.1:+14280258 | MS.gene044565:CDS |
| GTCTGTGAATTGCACTGATG+TGG | 0.605194 | 8.1:-14280412 | None:intergenic |
| GGTGATTCTAAATCTCATCT+GGG | 0.606736 | 8.1:+14278146 | MS.gene044565:CDS |
| GAAGCTACGACAGGAAAAGA+GGG | 0.609436 | 8.1:+14275942 | MS.gene044565:CDS |
| ATGGGTTAGAGAAGAAGACT+TGG | 0.611007 | 8.1:+14274927 | MS.gene044565:CDS |
| GCAGCTAATGGAGATAATGC+TGG | 0.617199 | 8.1:+14278125 | MS.gene044565:CDS |
| GCCATACAGCGGAGATACCA+AGG | 0.618161 | 8.1:+14278187 | MS.gene044565:CDS |
| CGACGAGGACGATATCGACT+GGG | 0.621959 | 8.1:+14282573 | MS.gene044565:CDS |
| CACTAGAGATCATAGGAGAG+AGG | 0.622575 | 8.1:+14275020 | MS.gene044565:CDS |
| CTGCTGTCTAGATTATGCAC+AGG | 0.622608 | 8.1:+14275998 | MS.gene044565:CDS |
| GCTACAGCTGAAGCTACGAC+AGG | 0.624340 | 8.1:+14275933 | MS.gene044565:CDS |
| TAAATTAGCTGCTCAAGACG+GGG | 0.624663 | 8.1:+14276516 | MS.gene044565:CDS |
| AGATGATGATGGTACCGAGT+GGG | 0.630560 | 8.1:+14280766 | MS.gene044565:CDS |
| TTCAATGGTACTGAAGGCAA+AGG | 0.631073 | 8.1:+14280252 | MS.gene044565:CDS |
| GAGCGCGTCAAGAACAACTA+CGG | 0.640458 | 8.1:-14274796 | None:intergenic |
| TGGTACTGAAGGCAAAGGAG+AGG | 0.643856 | 8.1:+14280257 | MS.gene044565:CDS |
| ATGAGAATGCACGAAGACGA+CGG | 0.644965 | 8.1:+14276557 | MS.gene044565:CDS |
| TACGAAAGAACAGCGTGGAG+AGG | 0.645484 | 8.1:+14280353 | MS.gene044565:CDS |
| TTTGTTGCAGATATATGATG+TGG | 0.649334 | 8.1:+14276188 | MS.gene044565:intron |
| AGCAACTTCGTCGAACTCTG+CGG | 0.650459 | 8.1:+14274974 | MS.gene044565:CDS |
| GAATATATATGTACAAACTG+TGG | 0.654824 | 8.1:+14276279 | MS.gene044565:CDS |
| TGGCTTGAAATCAAAACGTG+AGG | 0.659414 | 8.1:+14280742 | MS.gene044565:CDS |
| TAGAGAAGAAGACTTGGCAA+AGG | 0.661782 | 8.1:+14274933 | MS.gene044565:CDS |
| GGTGTTAAATCTGAAACTGA+CGG | 0.664295 | 8.1:+14280279 | MS.gene044565:CDS |
| AAATTAGCTGCTCAAGACGG+GGG | 0.670137 | 8.1:+14276517 | MS.gene044565:CDS |
| TGCAATTGGAGCTTACAAGG+TGG | 0.682571 | 8.1:+14282513 | MS.gene044565:CDS |
| GGACATGCTTCAGAAGATGG+AGG | 0.689485 | 8.1:+14276594 | MS.gene044565:CDS |
| ACGTTGTAATGGAAAACTAG+AGG | 0.692315 | 8.1:+14276483 | MS.gene044565:CDS |
| GGAGCACCATTGCCATACAG+CGG | 0.718354 | 8.1:+14278176 | MS.gene044565:CDS |
| AAGATGATGATGGTACCGAG+TGG | 0.721118 | 8.1:+14280765 | MS.gene044565:CDS |
| ACTGGAAGAAGTGATAACAG+AGG | 0.728511 | 8.1:+14274768 | MS.gene044565:CDS |
| TTCCATTACAACGTTCACAG+TGG | 0.729270 | 8.1:-14276474 | None:intergenic |
| CGAGGACGATATCGACTGGG+AGG | 0.742122 | 8.1:+14282576 | MS.gene044565:CDS |
| CCTGATGTAATCATCCATGG+AGG | 0.742710 | 8.1:-14280321 | None:intergenic |
| AATCTTACGAAAGAACAGCG+TGG | 0.751641 | 8.1:+14280348 | MS.gene044565:CDS |
| TCAGCCAGAAGAAGACGACG+AGG | 0.751885 | 8.1:+14282558 | MS.gene044565:CDS |
| ACAATGCTTTGGATGCACTG+CGG | 0.757429 | 8.1:+14276425 | MS.gene044565:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 14274578 | 14282606 | 14274578 | ID=MS.gene044565 |
| chr8.1 | mRNA | 14274578 | 14282606 | 14274578 | ID=MS.gene044565.t1;Parent=MS.gene044565 |
| chr8.1 | exon | 14274578 | 14274597 | 14274578 | ID=MS.gene044565.t1.exon1;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14274578 | 14274597 | 14274578 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| chr8.1 | exon | 14274701 | 14274823 | 14274701 | ID=MS.gene044565.t1.exon2;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14274701 | 14274823 | 14274701 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| chr8.1 | exon | 14274921 | 14275041 | 14274921 | ID=MS.gene044565.t1.exon3;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14274921 | 14275041 | 14274921 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| chr8.1 | exon | 14275894 | 14276019 | 14275894 | ID=MS.gene044565.t1.exon4;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14275894 | 14276019 | 14275894 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| chr8.1 | exon | 14276198 | 14276307 | 14276198 | ID=MS.gene044565.t1.exon5;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14276198 | 14276307 | 14276198 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| chr8.1 | exon | 14276423 | 14276615 | 14276423 | ID=MS.gene044565.t1.exon6;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14276423 | 14276615 | 14276423 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| chr8.1 | exon | 14278017 | 14278208 | 14278017 | ID=MS.gene044565.t1.exon7;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14278017 | 14278208 | 14278017 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| chr8.1 | exon | 14280240 | 14280476 | 14280240 | ID=MS.gene044565.t1.exon8;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14280240 | 14280476 | 14280240 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| chr8.1 | exon | 14280614 | 14280806 | 14280614 | ID=MS.gene044565.t1.exon9;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14280614 | 14280806 | 14280614 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| chr8.1 | exon | 14282509 | 14282606 | 14282509 | ID=MS.gene044565.t1.exon10;Parent=MS.gene044565.t1 |
| chr8.1 | CDS | 14282509 | 14282606 | 14282509 | ID=cds.MS.gene044565.t1;Parent=MS.gene044565.t1 |
| Gene Sequence |
| Protein sequence |
