Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045848.t1 | XP_013463904.1 | 98.4 | 377 | 6 | 0 | 1 | 377 | 1 | 377 | 3.60E-217 | 763.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045848.t1 | O81024 | 62.6 | 372 | 137 | 2 | 1 | 372 | 1 | 370 | 3.5e-136 | 486.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045848.t1 | A0A072V7D3 | 98.4 | 377 | 6 | 0 | 1 | 377 | 1 | 377 | 2.6e-217 | 763.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene045848 | MS.gene046260 | PPI |
| MS.gene045848 | MS.gene063832 | PPI |
| MS.gene016513 | MS.gene045848 | PPI |
| MS.gene045848 | MS.gene87205 | PPI |
| MS.gene045848 | MS.gene039577 | PPI |
| MS.gene069299 | MS.gene045848 | PPI |
| MS.gene045848 | MS.gene069952 | PPI |
| MS.gene045848 | MS.gene88406 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045848.t1 | MTR_2g054370 | 98.408 | 377 | 6 | 0 | 1 | 377 | 1 | 377 | 0.0 | 773 |
| MS.gene045848.t1 | MTR_3g013430 | 34.688 | 320 | 194 | 5 | 56 | 366 | 140 | 453 | 3.13e-55 | 189 |
| MS.gene045848.t1 | MTR_6g039820 | 34.056 | 323 | 190 | 6 | 59 | 366 | 29 | 343 | 3.39e-54 | 182 |
| MS.gene045848.t1 | MTR_8g041330 | 33.537 | 328 | 203 | 6 | 48 | 366 | 41 | 362 | 4.49e-54 | 184 |
| MS.gene045848.t1 | MTR_1g097850 | 32.923 | 325 | 179 | 8 | 57 | 365 | 27 | 328 | 2.79e-48 | 167 |
| MS.gene045848.t1 | MTR_6g040230 | 35.470 | 234 | 134 | 4 | 142 | 366 | 271 | 496 | 4.41e-39 | 145 |
| MS.gene045848.t1 | MTR_1g097850 | 31.276 | 243 | 133 | 6 | 57 | 285 | 27 | 249 | 3.86e-30 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene045848.t1 | AT2G26830 | 63.897 | 349 | 126 | 0 | 24 | 372 | 22 | 370 | 4.12e-171 | 482 |
| MS.gene045848.t1 | AT4G09760 | 35.577 | 312 | 185 | 6 | 64 | 366 | 38 | 342 | 4.18e-56 | 187 |
| MS.gene045848.t1 | AT4G09760 | 35.577 | 312 | 185 | 6 | 64 | 366 | 38 | 342 | 4.18e-56 | 187 |
| MS.gene045848.t1 | AT1G74320 | 32.209 | 326 | 198 | 9 | 56 | 368 | 32 | 347 | 4.34e-53 | 179 |
| MS.gene045848.t1 | AT1G71697 | 29.769 | 346 | 223 | 7 | 31 | 365 | 8 | 344 | 4.69e-48 | 166 |
| MS.gene045848.t1 | AT4G09760 | 30.208 | 192 | 126 | 4 | 64 | 253 | 38 | 223 | 5.47e-22 | 94.4 |
| MS.gene045848.t1 | AT4G09760 | 29.143 | 175 | 116 | 4 | 64 | 236 | 38 | 206 | 6.33e-18 | 82.0 |
Find 72 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAGATATCATTCCATAAT+TGG | 0.099867 | 2.4:-34927009 | None:intergenic |
| TGATATTGTTCGTTCATATT+TGG | 0.100547 | 2.4:-34932791 | None:intergenic |
| TCTCACAATGACTTGCTTTC+TGG | 0.116891 | 2.4:+34931619 | MS.gene045848:CDS |
| TTGCAGTTGTTTGGCTATTT+TGG | 0.127146 | 2.4:-34926948 | None:intergenic |
| GCAGCATCTGTTCTAGAATT+TGG | 0.157391 | 2.4:+34928352 | MS.gene045848:intron |
| TTTAAAGTTGAGAAAATTTC+TGG | 0.169623 | 2.4:+34924407 | MS.gene045848:CDS |
| GCTAAGTGGCTTGGAATATT+TGG | 0.191747 | 2.4:+34925801 | MS.gene045848:CDS |
| CCCAATTATGGAATGATATC+TGG | 0.258364 | 2.4:+34927008 | MS.gene045848:CDS |
| TCTACTCATTCCTTTCTAAA+AGG | 0.267387 | 2.4:-34934133 | None:intergenic |
| GATATTGTTCGTTCATATTT+GGG | 0.276266 | 2.4:-34932790 | None:intergenic |
| CCAGATATCATTCCATAATT+GGG | 0.278940 | 2.4:-34927008 | None:intergenic |
| TACATCACTGCTGCAGGATT+TGG | 0.299530 | 2.4:+34925777 | MS.gene045848:CDS |
| CCAATAGAATTTGATTATCT+TGG | 0.305896 | 2.4:+34934041 | MS.gene045848:CDS |
| CAAGATAATCAAATTCTATT+GGG | 0.308596 | 2.4:-34934040 | None:intergenic |
| GCTCTAAGGAACCCCAATTA+TGG | 0.311734 | 2.4:+34926996 | MS.gene045848:CDS |
| CCAAGATAATCAAATTCTAT+TGG | 0.314209 | 2.4:-34934041 | None:intergenic |
| TCAATGATATGCTCGGTGTT+GGG | 0.351100 | 2.4:-34924717 | None:intergenic |
| TTCAAGACCTGGTCAAGTTT+AGG | 0.351629 | 2.4:+34924374 | MS.gene045848:CDS |
| ACGAACAATATCATTTCTTC+AGG | 0.383658 | 2.4:+34932801 | MS.gene045848:CDS |
| GCTTGTTCTGCGATTTCAAC+GGG | 0.403905 | 2.4:-34923744 | None:intergenic |
| TGATCGCTTTCGAGAATTGC+AGG | 0.404652 | 2.4:+34924737 | MS.gene045848:CDS |
| TGTGGAAATTCCAGGCTCTA+AGG | 0.418229 | 2.4:+34926982 | MS.gene045848:CDS |
| ATTACCTCTTGAGGTCTGTC+TGG | 0.424572 | 2.4:-34932836 | None:intergenic |
| TGGCTTGGAATATTTGGAAA+TGG | 0.427165 | 2.4:+34925807 | MS.gene045848:CDS |
| ATCAATGATATGCTCGGTGT+TGG | 0.428005 | 2.4:-34924718 | None:intergenic |
| ATTATAACCATCAGATTGTA+TGG | 0.431135 | 2.4:+34924693 | MS.gene045848:CDS |
| AAGGGAAAGAGAAGGATCAA+TGG | 0.457811 | 2.4:-34923808 | None:intergenic |
| ATCAAATACATCACTGCTGC+AGG | 0.459460 | 2.4:+34925771 | MS.gene045848:CDS |
| TGGGTGGAAAAGTTAAGATC+TGG | 0.470594 | 2.4:+34923718 | MS.gene045848:CDS |
| GCGCATACTCTTGCTCCATC+AGG | 0.478124 | 2.4:+34925852 | MS.gene045848:CDS |
| AGATATGCGAGAACCAAAAC+TGG | 0.481384 | 2.4:+34926922 | MS.gene045848:intron |
| AATTGGGGTTCCTTAGAGCC+TGG | 0.484263 | 2.4:-34926992 | None:intergenic |
| TTCGATACAACGAGTACAAA+AGG | 0.487153 | 2.4:+34934075 | MS.gene045848:CDS |
| AGCTTGTTCTGCGATTTCAA+CGG | 0.491199 | 2.4:-34923745 | None:intergenic |
| TTACAAGACAGTTTCATTCA+AGG | 0.491822 | 2.4:+34928396 | MS.gene045848:CDS |
| AACTACAGAGGCTATGACAT+AGG | 0.495161 | 2.4:+34932605 | MS.gene045848:CDS |
| TCTTAAAACTTTGTATGTTG+AGG | 0.497216 | 2.4:+34933863 | MS.gene045848:CDS |
| TGATGAAATTTATGAGCTCC+AGG | 0.497219 | 2.4:+34928426 | MS.gene045848:CDS |
| TTTCACGGTGTGGAAATTCC+AGG | 0.499815 | 2.4:+34926974 | MS.gene045848:CDS |
| TGCAGCAGTGATGTATTTGA+TGG | 0.502189 | 2.4:-34925769 | None:intergenic |
| TTGAAATCGCAGAACAAGCT+CGG | 0.518498 | 2.4:+34923748 | MS.gene045848:CDS |
| GGATCAATGGAGAGAAGAGA+AGG | 0.518927 | 2.4:-34923795 | None:intergenic |
| CAATGATATGCTCGGTGTTG+GGG | 0.521995 | 2.4:-34924716 | None:intergenic |
| ATGACGAGAGGTGTCATTTG+AGG | 0.522794 | 2.4:-34923831 | None:intergenic |
| GGATTTGGTGCTAAGTGGCT+TGG | 0.523784 | 2.4:+34925792 | MS.gene045848:CDS |
| CTGCAGGATTTGGTGCTAAG+TGG | 0.526710 | 2.4:+34925787 | MS.gene045848:CDS |
| GAAAGAAAAGCTTGTATCGA+TGG | 0.527863 | 2.4:+34934100 | MS.gene045848:CDS |
| AATCACTTTGCAGAATATGC+TGG | 0.528361 | 2.4:+34932629 | MS.gene045848:CDS |
| CTGAAGGTTTCAGTTAAACA+AGG | 0.534256 | 2.4:+34924654 | MS.gene045848:CDS |
| CAGATATCATTCCATAATTG+GGG | 0.535171 | 2.4:-34927007 | None:intergenic |
| ATAATGATTAATCATGAAGA+AGG | 0.535615 | 2.4:+34931646 | MS.gene045848:CDS |
| CGAGAGGTGTCATTTGAGGA+AGG | 0.544148 | 2.4:-34923827 | None:intergenic |
| ACTACAGAGGCTATGACATA+GGG | 0.549695 | 2.4:+34932606 | MS.gene045848:CDS |
| ACAGCCAGACAGACCTCAAG+AGG | 0.575109 | 2.4:+34932832 | MS.gene045848:CDS |
| TGAATGCAGTAATTTGTGCA+AGG | 0.578273 | 2.4:+34924346 | MS.gene045848:intron |
| TGAGGTAAATACATATGCAC+TGG | 0.582931 | 2.4:+34933881 | MS.gene045848:CDS |
| ACTGTGATTATTACCTCTTG+AGG | 0.585155 | 2.4:-34932845 | None:intergenic |
| CTCATTCCTTTCTAAAAGGT+AGG | 0.596387 | 2.4:-34934129 | None:intergenic |
| AAGATAATCAAATTCTATTG+GGG | 0.603378 | 2.4:-34934039 | None:intergenic |
| AAAGCGATCAATGATATGCT+CGG | 0.607018 | 2.4:-34924724 | None:intergenic |
| GAGAGGTGTCATTTGAGGAA+GGG | 0.608013 | 2.4:-34923826 | None:intergenic |
| ATTTGAGGAAGGGAAAGAGA+AGG | 0.609170 | 2.4:-34923816 | None:intergenic |
| AAAGTTGAGAAAATTTCTGG+AGG | 0.619890 | 2.4:+34924410 | MS.gene045848:CDS |
| GCAAGGACATGTTCAAGACC+TGG | 0.621936 | 2.4:+34924363 | MS.gene045848:CDS |
| GTTGGGGCCATACAATCTGA+TGG | 0.622325 | 2.4:-34924700 | None:intergenic |
| AGAATCACCTAAACTTGACC+AGG | 0.623881 | 2.4:-34924381 | None:intergenic |
| GAAGGTTTCAGTTAAACAAG+GGG | 0.631491 | 2.4:+34924656 | MS.gene045848:CDS |
| AATATAATACTAATAATGGG+TGG | 0.636817 | 2.4:+34923702 | None:intergenic |
| TGAAGGTTTCAGTTAAACAA+GGG | 0.637167 | 2.4:+34924655 | MS.gene045848:CDS |
| AGAGTTACTGACATGACGAG+AGG | 0.684298 | 2.4:-34923843 | None:intergenic |
| CAGAGACCAGAAAAGGTGTG+AGG | 0.696052 | 2.4:-34933904 | None:intergenic |
| TATGCATCATACAACTACAG+AGG | 0.713439 | 2.4:+34932593 | MS.gene045848:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 34923717 | 34934154 | 34923717 | ID=MS.gene045848 |
| chr2.4 | mRNA | 34923717 | 34934154 | 34923717 | ID=MS.gene045848.t1;Parent=MS.gene045848 |
| chr2.4 | exon | 34923717 | 34923853 | 34923717 | ID=MS.gene045848.t1.exon1;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34923717 | 34923853 | 34923717 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34924355 | 34924446 | 34924355 | ID=MS.gene045848.t1.exon2;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34924355 | 34924446 | 34924355 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34924652 | 34924758 | 34924652 | ID=MS.gene045848.t1.exon3;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34924652 | 34924758 | 34924652 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34925768 | 34925873 | 34925768 | ID=MS.gene045848.t1.exon4;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34925768 | 34925873 | 34925768 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34926924 | 34927046 | 34926924 | ID=MS.gene045848.t1.exon5;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34926924 | 34927046 | 34926924 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34928356 | 34928447 | 34928356 | ID=MS.gene045848.t1.exon6;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34928356 | 34928447 | 34928356 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34931583 | 34931667 | 34931583 | ID=MS.gene045848.t1.exon7;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34931583 | 34931667 | 34931583 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34932567 | 34932672 | 34932567 | ID=MS.gene045848.t1.exon8;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34932567 | 34932672 | 34932567 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34932787 | 34932853 | 34932787 | ID=MS.gene045848.t1.exon9;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34932787 | 34932853 | 34932787 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34933849 | 34933941 | 34933849 | ID=MS.gene045848.t1.exon10;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34933849 | 34933941 | 34933849 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| chr2.4 | exon | 34934029 | 34934154 | 34934029 | ID=MS.gene045848.t1.exon11;Parent=MS.gene045848.t1 |
| chr2.4 | CDS | 34934029 | 34934154 | 34934029 | ID=cds.MS.gene045848.t1;Parent=MS.gene045848.t1 |
| Gene Sequence |
| Protein sequence |
